                                SEQUENCE LISTING

<110> Pavlakis, George N.
      Felber, Barbara K.
      Valentin, Antonio
      Bergamaschi, Cristina
      The Government of the United States of America
         as represented by The Secretary of the
         Department of Health and Human Services

<120> Use of IL-15 to Increase Thymic Output
  and to Treat Lymphopenia
  

<130> 015280-581100PC

<140> WO Not yet assigned 
<141> Not yet assigned  

<150> US 61/234,152       
<151> 2009-08-14  

<150> US 61/234,155       
<151> 2009-08-14  

<160> 20

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 489
<212> DNA
<213> Homo sapiens

<220> 
<223> human wild-type interleukin-15 (IL-15)

<400> 1
atgagaattt cgaaaccaca tttgagaagt atttccatcc agtgctactt gtgtttactt 60
ctaaacagtc attttctaac tgaagctggc attcatgtct tcattttggg ctgtttcagt 120
gcagggcttc ctaaaacaga agccaactgg gtgaatgtaa taagtgattt gaaaaaaatt 180
gaagatctta ttcaatctat gcatattgat gctactttat atacggaaag tgatgttcac 240
cccagttgca aagtaacagc aatgaagtgc tttctcttgg agttacaagt tatttcactt 300
gagtctggag atgcaagtat tcatgataca gtagaaaatc tgatcatcct agcaaacaac 360
agtttgtctt ctaatgggaa tgtaacagaa tctggatgca aagaatgtga ggaactggag 420
gaaaaaaata ttaaagaatt tttgcagagt tttgtacata ttgtccaaat gttcatcaac 480
acttcttga                                                         489

<210> 2
<211> 162
<212> PRT
<213> Homo sapiens

<220> 
<223> human wild-type interleukin-15 (IL-15)

<400> 2
Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr
 1               5                  10                  15      
Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His
            20                  25                  30          
Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala
        35                  40                  45              
Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile
    50                  55                  60                  
Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His
65                  70                  75                  80  
Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln
                85                  90                  95      
Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu
            100                 105                 110         
Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val
        115                 120                 125             
Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile
    130                 135                 140                 
Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn
145                 150                 155                 160 
Thr Ser
        


<210> 3
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic improved human interleukin-15 (IL-15) (opt1)

<400> 3
atgcggatct cgaagccgca cctgcggtcg atatcgatcc agtgctacct gtgcctgctc 60
ctgaactcgc acttcctcac ggaggccggt atacacgtct tcatcctggg ctgcttctcg 120
gcggggctgc cgaagacgga ggcgaactgg gtgaacgtga tctcggacct gaagaagatc 180
gaggacctca tccagtcgat gcacatcgac gcgacgctgt acacggagtc ggacgtccac 240
ccgtcgtgca aggtcacggc gatgaagtgc ttcctcctgg agctccaagt catctcgctc 300
gagtcggggg acgcgtcgat ccacgacacg gtggagaacc tgatcatcct ggcgaacaac 360
tcgctgtcgt cgaacgggaa cgtcacggag tcgggctgca aggagtgcga ggagctggag 420
gagaagaaca tcaaggagtt cctgcagtcg ttcgtgcaca tcgtccagat gttcatcaac 480
acgtcgtga                                                         489

<210> 4
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic improved human interleukin-15 (IL-15) (opt2)

<400> 4
atgaggatca gcaagcccca cctgaggagc atcagcatcc agtgctacct gtgcctgctg 60
ctgaacagcc acttcctgac cgaggccggt atacacgtgt tcatcctggg ctgctttagc 120
gccggactgc ccaagaccga ggccaattgg gtgaacgtga tcagcgacct gaagaagatc 180
gaggacctca tccagagcat gcacatcgac gccaccctgt acaccgagag cgatgtgcac 240
cccagctgta aggtgaccgc catgaagtgc tttctgctgg agctgcaagt gatcagcctg 300
gagagcggcg acgccagcat ccacgacacc gtggagaacc tgatcatcct ggccaacaac 360
agcctgagca gcaacggcaa tgtgaccgag agcggctgta aggagtgtga ggagctggag 420
gagaagaaca tcaaggagtt tctgcagagc ttcgtgcaca tcgtgcagat gttcatcaac 480
accagctga                                                         489

<210> 5
<211> 1610
<212> DNA
<213> Homo sapiens

<220> 
<223> human interleukin-15 receptor, alpha (IL15RA),
      transcript variant 1 cDNA

<220> 
<221> CDS
<222> (83)..(886)
<223> interleukin-15 receptor, alpha (IL15RA), isoform 1 precursor

<400> 5
cccagagcag cgctcgccac ctccccccgg cctgggcagc gctcgcccgg ggagtccagc 60
ggtgtcctgt ggagctgccg ccatggcccc gcggcgggcg cgcggctgcc ggaccctcgg 120
tctcccggcg ctgctactgc tgctgctgct ccggccgccg gcgacgcggg gcatcacgtg 180
ccctcccccc atgtccgtgg aacacgcaga catctgggtc aagagctaca gcttgtactc 240
cagggagcgg tacatttgta actctggttt caagcgtaaa gccggcacgt ccagcctgac 300
ggagtgcgtg ttgaacaagg ccacgaatgt cgcccactgg acaaccccca gtctcaaatg 360
cattagagac cctgccctgg ttcaccaaag gccagcgcca ccctccacag taacgacggc 420
aggggtgacc ccacagccag agagcctctc cccttctgga aaagagcccg cagcttcatc 480
tcccagctca aacaacacag cggccacaac agcagctatt gtcccgggct cccagctgat 540
gccttcaaaa tcaccttcca caggaaccac agagataagc agtcatgagt cctcccacgg 600
caccccctct cagacaacag ccaagaactg ggaactcaca gcatccgcct cccaccagcc 660
gccaggtgtg tatccacagg gccacagcga caccactgtg gctatctcca cgtccactgt 720
cctgctgtgt gggctgagcg ctgtgtctct cctggcatgc tacctcaagt caaggcaaac 780
tcccccgctg gccagcgttg aaatggaagc catggaggct ctgccggtga cttgggggac 840
cagcagcaga gatgaagact tggaaaactg ctctcaccac ctatgaaact cggggaaacc 900
agcccagcta agtccggagt gaaggagcct ctctgcttta gctaaagacg actgagaaga 960
ggtgcaagga agcgggctcc aggagcaagc tcaccaggcc tctcagaagt cccagcagga 1020
tctcacggac tgccgggtcg gcgcctcctg cgcgagggag caggttctcc gcattcccat 1080
gggcaccacc tgcctgcctg tcgtgccttg gacccagggc ccagcttccc aggagagacc 1140
aaaggcttct gagcaggatt tttatttcat tacagtgtga gctgcctgga atacatgtgg 1200
taatgaaata aaaaccctgc cccgaatctt ccgtccctca tcctaacttt cagttcacag 1260
agaaaagtga catacccaaa gctctctgtc aattacaagg cttctcctgg cgtgggagac 1320
gtctacaggg aagacaccag cgtttgggct tctaaccacc ctgtctccag ctgctctgca 1380
cacatggaca gggacctggg aaaggtggga gagatgctga gcccagcgaa tcctctccat 1440
tgaaggattc aggaagaaga aaactcaact cagtgccatt ttacgaatat atgcgtttat 1500
atttatactt ccttgtctat tatatctata cattatatat tatttgtatt ttgacattgt 1560
accttgtata aacaaaataa aacatctatt ttcaatattt ttaaaatgca            1610

<210> 6
<211> 267
<212> PRT
<213> Homo sapiens

<220> 
<223> human interleukin-15 receptor, alpha (IL15RA),
      isoform 1 precursor

<400> 6
Met Ala Pro Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala
 1               5                  10                  15      
Leu Leu Leu Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr
            20                  25                  30          
Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser
        35                  40                  45              
Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys
    50                  55                  60                  
Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala
65                  70                  75                  80  
Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp
                85                  90                  95      
Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr
            100                 105                 110         
Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu
        115                 120                 125             
Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala
    130                 135                 140                 
Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr
145                 150                 155                 160 
Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser
                165                 170                 175     
Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln
            180                 185                 190         
Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr Val Ala Ile
        195                 200                 205             
Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser Leu Leu
    210                 215                 220                 
Ala Cys Tyr Leu Lys Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu
225                 230                 235                 240 
Met Glu Ala Met Glu Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg
                245                 250                 255     
Asp Glu Asp Leu Glu Asn Cys Ser His His Leu
            260                 265         


<210> 7
<211> 1843
<212> DNA
<213> Homo sapiens

<220> 
<223> human interleukin-15 receptor, alpha (IL15RA),
      transcript variant 2 cDNA

<220> 
<221> CDS
<222> (424)..(1119)
<223> interleukin-15 receptor, alpha (IL15RA), isoform 2

<400> 7
caggaattcg gcgaagtggc ggagctgggg ccccagcggg cgccgggggc cgcgggagcc 60
agcaggtggc gggggctgcg ctccgcccgg gccagagcgc accaggcagg tgcccgcgcc 120
tccgcaccgc ggcgacacct ccgcgggcac tcacccaggc cggccgctca caaccgagcg 180
cagggccgcg gagggagacc aggaaagccg aaggcggagc agctggaggc gaccagcgcc 240
gggcgaggtc aagtggatcc gagccgcaga gagggctgga gagagtctgc tctccgatga 300
ctttgcccac tctcttcgca gtggggacac cggaccgagt gcacactgga ggtcccagag 360
cacgacgagc gcggaggacc gggaggctcc cgggcttgcg tgggcatcac gtgccctccc 420
cccatgtccg tggaacacgc agacatctgg gtcaagagct acagcttgta ctccagggag 480
cggtacattt gtaactctgg tttcaagcgt aaagccggca cgtccagcct gacggagtgc 540
gtgttgaaca aggccacgaa tgtcgcccac tggacaaccc ccagtctcaa atgcattaga 600
gaccctgccc tggttcacca aaggccagcg ccaccctcca cagtaacgac ggcaggggtg 660
accccacagc cagagagcct ctccccttct ggaaaagagc ccgcagcttc atctcccagc 720
tcaaacaaca cagcggccac aacagcagct attgtcccgg gctcccagct gatgccttca 780
aaatcacctt ccacaggaac cacagagata agcagtcatg agtcctccca cggcaccccc 840
tctcagacaa cagccaagaa ctgggaactc acagcatccg cctcccacca gccgccaggt 900
gtgtatccac agggccacag cgacaccact gtggctatct ccacgtccac tgtcctgctg 960
tgtgggctga gcgctgtgtc tctcctggca tgctacctca agtcaaggca aactcccccg 1020
ctggccagcg ttgaaatgga agccatggag gctctgccgg tgacttgggg gaccagcagc 1080
agagatgaag acttggaaaa ctgctctcac cacctatgaa actcggggaa accagcccag 1140
ctaagtccgg agtgaaggag cctctctgct ttagctaaag acgactgaga agaggtgcaa 1200
ggaagcgggc tccaggagca agctcaccag gcctctcaga agtcccagca ggatctcacg 1260
gactgccggg tcggcgcctc ctgcgcgagg gagcaggttc tccgcattcc catgggcacc 1320
acctgcctgc ctgtcgtgcc ttggacccag ggcccagctt cccaggagag accaaaggct 1380
tctgagcagg atttttattt cattacagtg tgagctgcct ggaatacatg tggtaatgaa 1440
ataaaaaccc tgccccgaat cttccgtccc tcatcctaac tttcagttca cagagaaaag 1500
tgacataccc aaagctctct gtcaattaca aggcttctcc tggcgtggga gacgtctaca 1560
gggaagacac cagcgtttgg gcttctaacc accctgtctc cagctgctct gcacacatgg 1620
acagggacct gggaaaggtg ggagagatgc tgagcccagc gaatcctctc cattgaagga 1680
ttcaggaaga agaaaactca actcagtgcc attttacgaa tatatgcgtt tatatttata 1740
cttccttgtc tattatatct atacattata tattatttgt attttgacat tgtaccttgt 1800
ataaacaaaa taaaacatct attttcaata tttttaaaat gca                   1843

<210> 8
<211> 231
<212> PRT
<213> Homo sapiens

<220> 
<223> human interleukin-15 receptor, alpha (IL15RA),
      isoform 2

<400> 8
Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser Tyr Ser Leu Tyr
 1               5                  10                  15      
Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys Arg Lys Ala Gly
            20                  25                  30          
Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala Thr Asn Val Ala
        35                  40                  45              
His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp Pro Ala Leu Val
    50                  55                  60                  
His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr Ala Gly Val Thr
65                  70                  75                  80  
Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu Pro Ala Ala Ser
                85                  90                  95      
Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala Ala Ile Val Pro
            100                 105                 110         
Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr Gly Thr Thr Glu
        115                 120                 125             
Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser Gln Thr Thr Ala
    130                 135                 140                 
Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln Pro Pro Gly Val
145                 150                 155                 160 
Tyr Pro Gln Gly His Ser Asp Thr Thr Val Ala Ile Ser Thr Ser Thr
                165                 170                 175     
Val Leu Leu Cys Gly Leu Ser Ala Val Ser Leu Leu Ala Cys Tyr Leu
            180                 185                 190         
Lys Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu Met Glu Ala Met
        195                 200                 205             
Glu Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg Asp Glu Asp Leu
    210                 215                 220                 
Glu Asn Cys Ser His His Leu
225                 230     


<210> 9
<211> 807
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic improved human interleukin-15 (IL-15)
      receptor alpha (IL15Ra), transcript variant 1 (OPT)

<400> 9
atggccccga ggcgggcgcg aggctgccgg accctcggtc tcccggcgct gctactgctc 60
ctgctgctcc ggccgccggc gacgcggggc atcacgtgcc cgccccccat gtccgtggag 120
cacgcagaca tctgggtcaa gagctacagc ttgtactccc gggagcggta catctgcaac 180
tcgggtttca agcggaaggc cggcacgtcc agcctgacgg agtgcgtgtt gaacaaggcc 240
acgaatgtcg cccactggac gaccccctcg ctcaagtgca tccgcgaccc ggccctggtt 300
caccagcggc ccgcgccacc ctccaccgta acgacggcgg gggtgacccc gcagccggag 360
agcctctccc cgtcgggaaa ggagcccgcc gcgtcgtcgc ccagctcgaa caacacggcg 420
gccacaactg cagcgatcgt cccgggctcc cagctgatgc cgtcgaagtc gccgtccacg 480
ggaaccacgg agatcagcag tcatgagtcc tcccacggca ccccctcgca aacgacggcc 540
aagaactggg aactcacggc gtccgcctcc caccagccgc cgggggtgta tccgcaaggc 600
cacagcgaca ccacggtggc gatctccacg tccacggtcc tgctgtgtgg gctgagcgcg 660
gtgtcgctcc tggcgtgcta cctcaagtcg aggcagactc ccccgctggc cagcgttgag 720
atggaggcca tggaggctct gccggtgacg tgggggacca gcagcaggga tgaggacttg 780
gagaactgct cgcaccacct ataatga                                     807

<210> 10
<211> 267
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic improved human interleukin-15 (IL-15)
      receptor alpha (IL15Ra), isoform 1 (OPT)

<400> 10
Met Ala Pro Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala
 1               5                  10                  15      
Leu Leu Leu Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr
            20                  25                  30          
Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser
        35                  40                  45              
Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys
    50                  55                  60                  
Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala
65                  70                  75                  80  
Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp
                85                  90                  95      
Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr
            100                 105                 110         
Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu
        115                 120                 125             
Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala
    130                 135                 140                 
Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr
145                 150                 155                 160 
Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser
                165                 170                 175     
Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln
            180                 185                 190         
Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr Val Ala Ile
        195                 200                 205             
Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser Leu Leu
    210                 215                 220                 
Ala Cys Tyr Leu Lys Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu
225                 230                 235                 240 
Met Glu Ala Met Glu Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg
                245                 250                 255     
Asp Glu Asp Leu Glu Asn Cys Ser His His Leu
            260                 265         


<210> 11
<211> 621
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic improved human soluble interleukin-15
      (IL-15) receptor alpha (IL-15sRa) (OPT)

<400> 11
atggccccga ggcgggcgcg aggctgccgg accctcggtc tcccggcgct gctactgctc 60
ctgctgctcc ggccgccggc gacgcggggc atcacgtgcc cgccccccat gtccgtggag 120
cacgcagaca tctgggtcaa gagctacagc ttgtactccc gggagcggta catctgcaac 180
tcgggtttca agcggaaggc cggcacgtcc agcctgacgg agtgcgtgtt gaacaaggcc 240
acgaatgtcg cccactggac gaccccctcg ctcaagtgca tccgcgaccc ggccctggtt 300
caccagcggc ccgcgccacc ctccaccgta acgacggcgg gggtgacccc gcagccggag 360
agcctctccc cgtcgggaaa ggagcccgcc gcgtcgtcgc ccagctcgaa caacacggcg 420
gccacaactg cagcgatcgt cccgggctcc cagctgatgc cgtcgaagtc gccgtccacg 480
ggaaccacgg agatcagcag tcatgagtcc tcccacggca ccccctcgca aacgacggcc 540
aagaactggg aactcacggc gtccgcctcc caccagccgc cgggggtgta tccgcaaggc 600
cacagcgaca ccacgtaatg a                                           621

<210> 12
<211> 205
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic improved human soluble interleukin-15
      (IL-15) receptor alpha (IL-15sRa) (OPT)

<400> 12
Met Ala Pro Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala
 1               5                  10                  15      
Leu Leu Leu Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr
            20                  25                  30          
Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser
        35                  40                  45              
Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys
    50                  55                  60                  
Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala
65                  70                  75                  80  
Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp
                85                  90                  95      
Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr
            100                 105                 110         
Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu
        115                 120                 125             
Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala
    130                 135                 140                 
Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr
145                 150                 155                 160 
Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser
                165                 170                 175     
Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln
            180                 185                 190         
Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr
        195                 200                 205 


<210> 13
<211> 5958
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic dual expression plasmid vector human
      IL15Ra+IL15

<400> 13
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720
gaagacaccg ggaccgatcc agcctccgcg ggcgcgcgtc gaggaattcg ctagcaagaa 780
atggccccga ggcgggcgcg aggctgccgg accctcggtc tcccggcgct gctactgctc 840
ctgctgctcc ggccgccggc gacgcggggc atcacgtgcc cgccccccat gtccgtggag 900
cacgcagaca tctgggtcaa gagctacagc ttgtactccc gggagcggta catctgcaac 960
tcgggtttca agcggaaggc cggcacgtcc agcctgacgg agtgcgtgtt gaacaaggcc 1020
acgaatgtcg cccactggac gaccccctcg ctcaagtgca tccgcgaccc ggccctggtt 1080
caccagcggc ccgcgccacc ctccaccgta acgacggcgg gggtgacccc gcagccggag 1140
agcctctccc cgtcgggaaa ggagcccgcc gcgtcgtcgc ccagctcgaa caacacggcg 1200
gccacaactg cagcgatcgt cccgggctcc cagctgatgc cgtcgaagtc gccgtccacg 1260
ggaaccacgg agatcagcag tcatgagtcc tcccacggca ccccctcgca aacgacggcc 1320
aagaactggg aactcacggc gtccgcctcc caccagccgc cgggggtgta tccgcaaggc 1380
cacagcgaca ccacggtggc gatctccacg tccacggtcc tgctgtgtgg gctgagcgcg 1440
gtgtcgctcc tggcgtgcta cctcaagtcg aggcagactc ccccgctggc cagcgttgag 1500
atggaggcca tggaggctct gccggtgacg tgggggacca gcagcaggga tgaggacttg 1560
gagaactgct cgcaccacct ataatgagaa ttcacgcgtg gatctgatat cggatctgct 1620
gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 1680
gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 1740
agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 1800
gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtaccca ggtgctgaag 1860
aattgacccg gttcctcctg ggccagaaag aagcaggcac atccccttct ctgtgacaca 1920
ccctgtccac gcccctggtt cttagttcca gccccactca taggacactc atagctcagg 1980
agggctccgc cttcaatccc acccgctaaa gtacttggag cggtctctcc ctccctcatc 2040
agcccaccaa accaaaccta gcctccaaga gtgggaagaa attaaagcaa gataggctat 2100
taagtgcaga gggagagaaa atgcctccaa catgtgagga agtaatgaga gaaatcatag 2160
aatttcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2220
ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2280
aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2340
ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2400
gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2460
cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2520
gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2580
tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2640
cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2700
cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2760
gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 2820
agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2880
cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2940
tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3000
tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3060
ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3120
cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcgg 3180
gggggggggg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg 3240
aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta 3300
ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg 3360
aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg 3420
tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag 3480
aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca 3540
tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg 3600
atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt 3660
aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa 3720
tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca 3780
ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc 3840
tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc 3900
aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct 3960
tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca 4020
ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt 4080
ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac 4140
tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta 4200
tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc 4260
gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa 4320
gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga 4380
ttttgagaca caacgtggat catccagaca tgataagata cattgatgag tttggacaaa 4440
ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 4500
tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 4560
tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat 4620
gtggtatggc tgattatgat cgtcgaggat ctggatccgt taaccgatat ccgcgaattc 4680
ggcgcgccgg gccctcacga cgtgttgatg aacatctgga cgatgtgcac gaacgactgc 4740
aggaactcct tgatgttctt ctcctccagc tcctcgcact ccttgcagcc cgactccgtg 4800
acgttcccgt tcgacgacag cgagttgttc gccaggatga tcaggttctc caccgtgtcg 4860
tggatcgacg cgtcccccga ctcgagcgag atgacttgga gctccaggag gaagcacttc 4920
atcgccgtga ccttgcacga cgggtggacg tccgactccg tgtacagcgt cgcgtcgatg 4980
tgcatcgact ggatgaggtc ctcgatcttc ttcaggtccg agatcacgtt cacccagttc 5040
gcctccgtct tcggcagccc cgccgagaag cagcccagga tgaagacgtg tataccggcc 5100
tccgtgagga agtgcgagtt caggagcagg cacaggtagc actggatcga tatcgaccgc 5160
aggtgcggct tcgagatccg catttcttgt cgacactcga cagatccaaa cgctcctccg 5220
acgtccccag gcagaatggc ggttccctaa acgagcattg cttatataga cctcccatta 5280
ggcacgccta ccgcccattt acgtcaatgg aacgcccatt tgcgtcattg cccctcccca 5340
ttgacgtcaa tggggatgta cttggcagcc atcgcgggcc atttaccgcc attgacgtca 5400
atgggagtac tgccaatgta ccctggcgta cttccaatag taatgtactt gccaagttac 5460
tattaataga tattgatgta ctgccaagtg ggccatttac cgtcattgac gtcaataggg 5520
ggcgtgagaa cggatatgaa tgggcaatga gccatcccat tgacgtcaat ggtgggtggt 5580
cctattgacg tcaatgggca ttgagccagg cgggccattt accgtaattg acgtcaatgg 5640
gggaggcgcc atatacgtca ataggaccgc ccatatgacg tcaataggaa agaccatgag 5700
gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 5760
ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 5820
gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 5880
actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 5940
catcagattg gctattgg                                               5958

<210> 14
<211> 5918
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic dual expression plasmid vector human
      IL15Ra+IL15tPA6

<400> 14
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720
gaagacaccg ggaccgatcc agcctccgcg ggcgcgcgtc gaggaattcg ctagcaagaa 780
atggccccga ggcgggcgcg aggctgccgg accctcggtc tcccggcgct gctactgctc 840
ctgctgctcc ggccgccggc gacgcggggc atcacgtgcc cgccccccat gtccgtggag 900
cacgcagaca tctgggtcaa gagctacagc ttgtactccc gggagcggta catctgcaac 960
tcgggtttca agcggaaggc cggcacgtcc agcctgacgg agtgcgtgtt gaacaaggcc 1020
acgaatgtcg cccactggac gaccccctcg ctcaagtgca tccgcgaccc ggccctggtt 1080
caccagcggc ccgcgccacc ctccaccgta acgacggcgg gggtgacccc gcagccggag 1140
agcctctccc cgtcgggaaa ggagcccgcc gcgtcgtcgc ccagctcgaa caacacggcg 1200
gccacaactg cagcgatcgt cccgggctcc cagctgatgc cgtcgaagtc gccgtccacg 1260
ggaaccacgg agatcagcag tcatgagtcc tcccacggca ccccctcgca aacgacggcc 1320
aagaactggg aactcacggc gtccgcctcc caccagccgc cgggggtgta tccgcaaggc 1380
cacagcgaca ccacggtggc gatctccacg tccacggtcc tgctgtgtgg gctgagcgcg 1440
gtgtcgctcc tggcgtgcta cctcaagtcg aggcagactc ccccgctggc cagcgttgag 1500
atggaggcca tggaggctct gccggtgacg tgggggacca gcagcaggga tgaggacttg 1560
gagaactgct cgcaccacct ataatgagaa ttcacgcgtg gatctgatat cggatctgct 1620
gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 1680
gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 1740
agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 1800
gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtaccca ggtgctgaag 1860
aattgacccg gttcctcctg ggccagaaag aagcaggcac atccccttct ctgtgacaca 1920
ccctgtccac gcccctggtt cttagttcca gccccactca taggacactc atagctcagg 1980
agggctccgc cttcaatccc acccgctaaa gtacttggag cggtctctcc ctccctcatc 2040
agcccaccaa accaaaccta gcctccaaga gtgggaagaa attaaagcaa gataggctat 2100
taagtgcaga gggagagaaa atgcctccaa catgtgagga agtaatgaga gaaatcatag 2160
aatttcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2220
ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2280
aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2340
ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2400
gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2460
cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2520
gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2580
tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2640
cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2700
cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2760
gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 2820
agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2880
cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2940
tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3000
tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3060
ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3120
cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcgg 3180
gggggggggg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg 3240
aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta 3300
ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg 3360
aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg 3420
tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag 3480
aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca 3540
tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg 3600
atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt 3660
aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa 3720
tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca 3780
ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc 3840
tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc 3900
aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct 3960
tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca 4020
ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt 4080
ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac 4140
tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta 4200
tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc 4260
gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa 4320
gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga 4380
ttttgagaca caacgtggat catccagaca tgataagata cattgatgag tttggacaaa 4440
ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 4500
tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 4560
tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat 4620
gtggtatggc tgattatgat cgtcgaggat ctggatctgg atccgttaac cgatatccgc 4680
gaattcggcg cgccgggccc tcacgacgtg ttgatgaaca tctggacgat gtgcacgaac 4740
gactgcagga actccttgat gttcttctcc tccagctcct cgcactcctt gcagcccgac 4800
tccgtgacgt tcccgttcga cgacagcgag ttgttcgcca ggatgatcag gttctccacc 4860
gtgtcgtgga tcgacgcgtc ccccgactcg agcgagatga cttggagctc caggaggaag 4920
cacttcatcg ccgtgacctt gcacgacggg tggacgtccg actccgtgta cagcgtcgcg 4980
tcgatgtgca tcgactggat gaggtcctcg atcttcttca ggtccgagat cacgttcacc 5040
cagtttctgg ctcctcttct gaatcgggca tggatttcct ggctgggcga aacgaagact 5100
gctccacaca gcagcagcac acagcagagc cctctcttca ttgcatccat ttcttgtcga 5160
cagatccaaa cgctcctccg acgtccccag gcagaatggc ggttccctaa acgagcattg 5220
cttatataga cctcccatta ggcacgccta ccgcccattt acgtcaatgg aacgcccatt 5280
tgcgtcattg cccctcccca ttgacgtcaa tggggatgta cttggcagcc atcgcgggcc 5340
atttaccgcc attgacgtca atgggagtac tgccaatgta ccctggcgta cttccaatag 5400
taatgtactt gccaagttac tattaataga tattgatgta ctgccaagtg ggccatttac 5460
cgtcattgac gtcaataggg ggcgtgagaa cggatatgaa tgggcaatga gccatcccat 5520
tgacgtcaat ggtgggtggt cctattgacg tcaatgggca ttgagccagg cgggccattt 5580
accgtaattg acgtcaatgg gggaggcgcc atatacgtca ataggaccgc ccatatgacg 5640
tcaataggaa agaccatgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 5700
ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 5760
agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 5820
gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 5880
tgcgtaagga gaaaataccg catcagattg gctattgg                         5918

<210> 15
<211> 5736
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic dual expression plasmid vector human
      IL15sRa(soluble)+IL15tPA6

<400> 15
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360
acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720
gaagacaccg ggaccgatcc agcctccgcg ggcgcgcgtc gaggaattcg ctagcaagaa 780
atggccccga ggcgggcgcg aggctgccgg accctcggtc tcccggcgct gctactgctc 840
ctgctgctcc ggccgccggc gacgcggggc atcacgtgcc cgccccccat gtccgtggag 900
cacgcagaca tctgggtcaa gagctacagc ttgtactccc gggagcggta catctgcaac 960
tcgggtttca agcggaaggc cggcacgtcc agcctgacgg agtgcgtgtt gaacaaggcc 1020
acgaatgtcg cccactggac gaccccctcg ctcaagtgca tccgcgaccc ggccctggtt 1080
caccagcggc ccgcgccacc ctccaccgta acgacggcgg gggtgacccc gcagccggag 1140
agcctctccc cgtcgggaaa ggagcccgcc gcgtcgtcgc ccagctcgaa caacacggcg 1200
gccacaactg cagcgatcgt cccgggctcc cagctgatgc cgtcgaagtc gccgtccacg 1260
ggaaccacgg agatcagcag tcatgagtcc tcccacggca ccccctcgca aacgacggcc 1320
aagaactggg aactcacggc gtccgcctcc caccagccgc cgggggtgta tccgcaaggc 1380
cacagcgaca ccacgtaatg agaattcgcg gatatcggtt aacggatcca gatctgctgt 1440
gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 1500
aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 1560
taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 1620
agacaatagc aggcatgctg gggatgcggt gggctctatg ggtacccagg tgctgaagaa 1680
ttgacccggt tcctcctggg ccagaaagaa gcaggcacat ccccttctct gtgacacacc 1740
ctgtccacgc ccctggttct tagttccagc cccactcata ggacactcat agctcaggag 1800
ggctccgcct tcaatcccac ccgctaaagt acttggagcg gtctctccct ccctcatcag 1860
cccaccaaac caaacctagc ctccaagagt gggaagaaat taaagcaaga taggctatta 1920
agtgcagagg gagagaaaat gcctccaaca tgtgaggaag taatgagaga aatcatagaa 1980
tttcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 2040
tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 2100
agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 2160
cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 2220
ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 2280
tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 2340
gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc 2400
gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 2460
gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 2520
ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 2580
ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 2640
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 2700
gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 2760
ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 2820
tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 2880
ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 2940
gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactcgggg 3000
ggggggggcg ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa 3060
tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg 3120
tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa 3180
gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccgccgtc 3240
ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa 3300
aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata 3360
tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat 3420
ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa 3480
tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc 3540
cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt 3600
acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg 3660
agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa 3720
ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc 3780
taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg 3840
agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct 3900
gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc 3960
tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc 4020
gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga 4080
gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc 4140
agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt 4200
ttgagacaca acgtggatca tccagacatg ataagataca ttgatgagtt tggacaaacc 4260
acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 4320
tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 4380
tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 4440
ggtatggctg attatgatcg tcgaggatct ggatctggat ccgttaaccg atatccgcga 4500
attcggcgcg ccgggccctc acgacgtgtt gatgaacatc tggacgatgt gcacgaacga 4560
ctgcaggaac tccttgatgt tcttctcctc cagctcctcg cactccttgc agcccgactc 4620
cgtgacgttc ccgttcgacg acagcgagtt gttcgccagg atgatcaggt tctccaccgt 4680
gtcgtggatc gacgcgtccc ccgactcgag cgagatgact tggagctcca ggaggaagca 4740
cttcatcgcc gtgaccttgc acgacgggtg gacgtccgac tccgtgtaca gcgtcgcgtc 4800
gatgtgcatc gactggatga ggtcctcgat cttcttcagg tccgagatca cgttcaccca 4860
gtttctggct cctcttctga atcgggcatg gatttcctgg ctgggcgaaa cgaagactgc 4920
tccacacagc agcagcacac agcagagccc tctcttcatt gcatccattt cttgtcgaca 4980
gatccaaacg ctcctccgac gtccccaggc agaatggcgg ttccctaaac gagcattgct 5040
tatatagacc tcccattagg cacgcctacc gcccatttac gtcaatggaa cgcccatttg 5100
cgtcattgcc cctccccatt gacgtcaatg gggatgtact tggcagccat cgcgggccat 5160
ttaccgccat tgacgtcaat gggagtactg ccaatgtacc ctggcgtact tccaatagta 5220
atgtacttgc caagttacta ttaatagata ttgatgtact gccaagtggg ccatttaccg 5280
tcattgacgt caataggggg cgtgagaacg gatatgaatg ggcaatgagc catcccattg 5340
acgtcaatgg tgggtggtcc tattgacgtc aatgggcatt gagccaggcg ggccatttac 5400
cgtaattgac gtcaatgggg gaggcgccat atacgtcaat aggaccgccc atatgacgtc 5460
aataggaaag accatgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 5520
ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 5580
acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 5640
ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 5700
cgtaaggaga aaataccgca tcagattggc tattgg                           5736

<210> 16
<211> 6369
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sIL-15Ralpha-Fc fusion protein plasmid
      vector DPhuIL15sRa205FC+huGMIL15

<400> 16
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360
atggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720
gaagacaccg ggaccgatcc agcctccgcg ggcgcgcgtc gacgctagca agaaatggcc 780
ccgaggcggg cgcgaggctg ccggaccctc ggtctcccgg cgctgctact gctcctgctg 840
ctccggccgc cggcgacgcg gggcatcacg tgcccgcccc ccatgtccgt ggagcacgca 900
gacatctggg tcaagagcta cagcttgtac tcccgggagc ggtacatctg caactcgggt 960
ttcaagcgga aggccggcac gtccagcctg acggagtgcg tgttgaacaa ggccacgaat 1020
gtcgcccact ggacgacccc ctcgctcaag tgcatccgcg acccggccct ggttcaccag 1080
cggcccgcgc caccctccac cgtaacgacg gcgggggtga ccccgcagcc ggagagcctc 1140
tccccgtcgg gaaaggagcc cgccgcgtcg tcgcccagct cgaacaacac ggcggccaca 1200
actgcagcga tcgtcccggg ctcccagctg atgccgtcga agtcgccgtc cacgggaacc 1260
acggagatca gcagtcatga gtcctcccac ggcaccccct cgcaaacgac ggccaagaac 1320
tgggaactca cggcgtccgc ctcccaccag ccgccggggg tgtatccgca aggccacagc 1380
gacaccacgc cgaagtcctg cgacaagacg cacacgtgcc ctccctgccc ggcgcccgag 1440
ctgctgggag gtccgagcgt gttcctcttc ccgcccaagc cgaaggacac gctcatgatc 1500
tcgcggactc ccgaggtcac ctgcgtcgtg gtagacgtca gccacgagga cccggaggtc 1560
aagttcaact ggtacgttga cggcgtagag gtgcacaacg cgaagacgaa gccgcgggag 1620
gagcagtaca actcgacgta ccgagtcgtg tcggtcctga ccgtcctgca ccaggactgg 1680
ctcaacggga aggagtacaa gtgcaaggtg tcgaacaagg cgctccctgc cccgatcgag 1740
aagacgatct cgaaggcgaa gggccagccc agggagcccc aggtctacac gctcccgcca 1800
tcgcgggacg agctgacgaa gaaccaggtt tccctgacgt gcctcgtcaa gggcttctac 1860
ccatcggaca tcgcggtgga gtgggagagc aacgggcagc cggagaacaa ctacaagacc 1920
acgcctccgg tgctcgactc ggacgggtcg ttcttcctct actcgaagct gaccgtcgac 1980
aagagccggt ggcagcaggg caacgtgttc tcctgctcgg tgatgcacga ggccctccac 2040
aaccactaca cccagaagtc gctcagtctg agcccgggga agtaatgagg atccgaattc 2100
gcggatatcg gttaacggat ccagatctgc tgtgccttct agttgccagc catctgttgt 2160
ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 2220
ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 2280
ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 2340
ggtgggctct atgggtaccc aggtgctgaa gaattgaccc ggttcctcct gggccagaaa 2400
gaagcaggca catccccttc tctgtgacac accctgtcca cgcccctggt tcttagttcc 2460
agccccactc ataggacact catagctcag gagggctccg ccttcaatcc cacccgctaa 2520
agtacttgga gcggtctctc cctccctcat cagcccacca aaccaaacct agcctccaag 2580
agtgggaaga aattaaagca agataggcta ttaagtgcag agggagagaa aatgcctcca 2640
acatgtgagg aagtaatgag agaaatcata gaatttcttc cgcttcctcg ctcactgact 2700
cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 2760
ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 2820
aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 2880
acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 2940
gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3000
ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3060
gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3120
cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3180
taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3240
atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 3300
cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3360
cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 3420
ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3480
ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 3540
tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 3600
aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 3660
tatttcgttc atccatagtt gcctgactcg gggggggggg gcgctgaggt ctgcctcgtg 3720
aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga 3780
gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt 3840
gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag 3900
caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca 3960
gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg 4020
caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 4080
aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 4140
tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 4200
aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat 4260
ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc 4320
aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt 4380
aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc 4440
aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg 4500
gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg 4560
aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc 4620
aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg 4680
atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc 4740
agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct 4800
cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat 4860
atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtgga tcatccagac 4920
atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 4980
tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 5040
caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 5100
gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcgtcgagga 5160
tctggatccg ttaaccgata tccgcgaatt cggcgcgccg ggccctcacg acgtgttgat 5220
gaacatctgg acgatgtgca cgaacgactg caggaactcc ttgatgttct tctcctccag 5280
ctcctcgcac tccttgcagc ccgactccgt gacgttcccg ttcgacgaca gcgagttgtt 5340
cgccaggatg atcaggttct ccaccgtgtc gtggatcgac gcgtcccccg actcgagcga 5400
gatgacttgg agctccagga ggaagcactt catcgccgtg accttgcacg acgggtggac 5460
gtccgactcc gtgtacagcg tcgcgtcgat gtgcatcgac tggatgaggt cctcgatctt 5520
cttcaggtcc gagatcacgt tcacccagtt cgagatgctg caggccaccg tccccaggag 5580
tagcaggctc tggagccaca tttcttgtcg acagatccaa acgctcctcc gacgtcccca 5640
ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt aggcacgcct 5700
accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gcccctcccc attgacgtca 5760
atggggatgt acttggcagc catcgcgggc catttaccgc cattgacgtc aatgggagta 5820
ctgccaatgt accctggcgt acttccaata gtaatgtact tgccaagtta ctattaatag 5880
atattgatgt actgccaagt gggccattta ccgtcattga cgtcaatagg gggcgtgaga 5940
acggatatga atgggcaatg agccatccca ttgacgtcaa tggtgggtgg tcctattgac 6000
gtcaatgggc attgagccag gcgggccatt taccgtaatt gacgtcaatg ggggaggcgc 6060
catatacgtc aataggaccg cccatatgac gtcaataggt aagaccatga ggccctttcg 6120
tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 6180
cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 6240
tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 6300
gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcagatt 6360
ggctattgg                                                         6369

<210> 17
<211> 436
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic sIL-15Ralpha-Fc fusion protein
      huIL15sRa205-Fc

<400> 17
Met Ala Pro Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala
 1               5                  10                  15      
Leu Leu Leu Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr
            20                  25                  30          
Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser
        35                  40                  45              
Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys
    50                  55                  60                  
Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala
65                  70                  75                  80  
Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp
                85                  90                  95      
Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr
            100                 105                 110         
Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu
        115                 120                 125             
Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala
    130                 135                 140                 
Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr
145                 150                 155                 160 
Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser
                165                 170                 175     
Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln
            180                 185                 190         
Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr Pro Lys Ser
        195                 200                 205             
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu
    210                 215                 220                 
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
225                 230                 235                 240 
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
                245                 250                 255     
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
            260                 265                 270         
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
        275                 280                 285             
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
    290                 295                 300                 
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
305                 310                 315                 320 
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
                325                 330                 335     
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
            340                 345                 350         
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
        355                 360                 365             
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
    370                 375                 380                 
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
385                 390                 395                 400 
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
                405                 410                 415     
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
            420                 425                 430         
Ser Pro Gly Lys
        435     


<210> 18
<211> 131
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic human GMCSF-IL15 fusion protein
      huGMCSF-IL15

<400> 18
Met Trp Leu Gln Ser Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile
 1               5                  10                  15      
Ser Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu
            20                  25                  30          
Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val
        35                  40                  45              
His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu
    50                  55                  60                  
Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val
65                  70                  75                  80  
Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn
                85                  90                  95      
Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn
            100                 105                 110         
Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile
        115                 120                 125             
Asn Thr Ser
    130     


<210> 19
<211> 6354
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic IL-15Ralpha-Fc fusion protein plasmid
      vector AG256DPhuIL15sRA200FC+huGMIL15

<400> 19
cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60
caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120
ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180
cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240
tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300
cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360
atggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420
ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480
tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540
tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600
ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660
ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720
gaagacaccg ggaccgatcc agcctccgcg ggcgcgcgtc gacgctagca agaaatggcc 780
ccgaggcggg cgcgaggctg ccggaccctc ggtctcccgg cgctgctact gctcctgctg 840
ctccggccgc cggcgacgcg gggcatcacg tgcccgcccc ccatgtccgt ggagcacgca 900
gacatctggg tcaagagcta cagcttgtac tcccgggagc ggtacatctg caactcgggt 960
ttcaagcgga aggccggcac gtccagcctg acggagtgcg tgttgaacaa ggccacgaat 1020
gtcgcccact ggacgacccc ctcgctcaag tgcatccgcg acccggccct ggttcaccag 1080
cggcccgcgc caccctccac cgtaacgacg gcgggggtga ccccgcagcc ggagagcctc 1140
tccccgtcgg gaaaggagcc cgccgcgtcg tcgcccagct cgaacaacac ggcggccaca 1200
actgcagcga tcgtcccggg ctcccagctg atgccgtcga agtcgccgtc cacgggaacc 1260
acggagatca gcagtcatga gtcctcccac ggcaccccct cgcaaacgac ggccaagaac 1320
tgggaactca cggcgtccgc ctcccaccag ccgccggggg tgtatccgca aggcccgaag 1380
tcctgcgaca agacgcacac gtgccctccc tgcccggcgc ccgagctgct gggaggtccg 1440
agcgtgttcc tcttcccgcc caagccgaag gacacgctca tgatctcgcg gactcccgag 1500
gtcacctgcg tcgtggtaga cgtcagccac gaggacccgg aggtcaagtt caactggtac 1560
gttgacggcg tagaggtgca caacgcgaag acgaagccgc gggaggagca gtacaactcg 1620
acgtaccgag tcgtgtcggt cctgaccgtc ctgcaccagg actggctcaa cgggaaggag 1680
tacaagtgca aggtgtcgaa caaggcgctc cctgccccga tcgagaagac gatctcgaag 1740
gcgaagggcc agcccaggga gccccaggtc tacacgctcc cgccatcgcg ggacgagctg 1800
acgaagaacc aggtttccct gacgtgcctc gtcaagggct tctacccatc ggacatcgcg 1860
gtggagtggg agagcaacgg gcagccggag aacaactaca agaccacgcc tccggtgctc 1920
gactcggacg ggtcgttctt cctctactcg aagctgaccg tcgacaagag ccggtggcag 1980
cagggcaacg tgttctcctg ctcggtgatg cacgaggccc tccacaacca ctacacccag 2040
aagtcgctca gtctgagccc ggggaagtaa tgaggatccg aattcgcgga tatcggttaa 2100
cggatccaga tctgctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2160
gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2220
tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2280
caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg 2340
tacccaggtg ctgaagaatt gacccggttc ctcctgggcc agaaagaagc aggcacatcc 2400
ccttctctgt gacacaccct gtccacgccc ctggttctta gttccagccc cactcatagg 2460
acactcatag ctcaggaggg ctccgccttc aatcccaccc gctaaagtac ttggagcggt 2520
ctctccctcc ctcatcagcc caccaaacca aacctagcct ccaagagtgg gaagaaatta 2580
aagcaagata ggctattaag tgcagaggga gagaaaatgc ctccaacatg tgaggaagta 2640
atgagagaaa tcatagaatt tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 2700
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 2760
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 2820
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 2880
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 2940
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 3000
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 3060
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 3120
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 3180
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 3240
cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 3300
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 3360
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 3420
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 3480
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 3540
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 3600
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 3660
tagttgcctg actcgggggg ggggggcgct gaggtctgcc tcgtgaagaa ggtgttgctg 3720
actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga 3780
tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac 3840
ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta 3900
ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt 3960
aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc 4020
aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc 4080
gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac 4140
atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc 4200
atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg 4260
ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt 4320
cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca 4380
aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc 4440
tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag 4500
taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc 4560
cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc 4620
atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc 4680
tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga 4740
atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt 4800
attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc 4860
aatgtaacat cagagatttt gagacacaac gtggatcatc cagacatgat aagatacatt 4920
gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 4980
tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 5040
aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag 5100
taaaacctct acaaatgtgg tatggctgat tatgatcgtc gaggatctgg atccgttaac 5160
cgatatccgc gaattcggcg cgccgggccc tcacgacgtg ttgatgaaca tctggacgat 5220
gtgcacgaac gactgcagga actccttgat gttcttctcc tccagctcct cgcactcctt 5280
gcagcccgac tccgtgacgt tcccgttcga cgacagcgag ttgttcgcca ggatgatcag 5340
gttctccacc gtgtcgtgga tcgacgcgtc ccccgactcg agcgagatga cttggagctc 5400
caggaggaag cacttcatcg ccgtgacctt gcacgacggg tggacgtccg actccgtgta 5460
cagcgtcgcg tcgatgtgca tcgactggat gaggtcctcg atcttcttca ggtccgagat 5520
cacgttcacc cagttcgaga tgctgcaggc caccgtcccc aggagtagca ggctctggag 5580
ccacatttct tgtcgacaga tccaaacgct cctccgacgt ccccaggcag aatggcggtt 5640
ccctaaacga gcattgctta tatagacctc ccattaggca cgcctaccgc ccatttacgt 5700
caatggaacg cccatttgcg tcattgcccc tccccattga cgtcaatggg gatgtacttg 5760
gcagccatcg cgggccattt accgccattg acgtcaatgg gagtactgcc aatgtaccct 5820
ggcgtacttc caatagtaat gtacttgcca agttactatt aatagatatt gatgtactgc 5880
caagtgggcc atttaccgtc attgacgtca atagggggcg tgagaacgga tatgaatggg 5940
caatgagcca tcccattgac gtcaatggtg ggtggtccta ttgacgtcaa tgggcattga 6000
gccaggcggg ccatttaccg taattgacgt caatggggga ggcgccatat acgtcaatag 6060
gaccgcccat atgacgtcaa taggtaagac catgaggccc tttcgtctcg cgcgtttcgg 6120
tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 6180
agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 6240
gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 6300
tgaaataccg cacagatgcg taaggagaaa ataccgcatc agattggcta ttgg       6354

<210> 20
<211> 431
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic sIL-15Ralpha-Fc fusion protein
      huIL15sRa200-Fc

<400> 20
Met Ala Pro Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala
 1               5                  10                  15      
Leu Leu Leu Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr
            20                  25                  30          
Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser
        35                  40                  45              
Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys
    50                  55                  60                  
Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala
65                  70                  75                  80  
Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp
                85                  90                  95      
Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr
            100                 105                 110         
Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu
        115                 120                 125             
Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala
    130                 135                 140                 
Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr
145                 150                 155                 160 
Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser
                165                 170                 175     
Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln
            180                 185                 190         
Pro Pro Gly Val Tyr Pro Gln Gly Pro Lys Ser Cys Asp Lys Thr His
        195                 200                 205             
Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val
    210                 215                 220                 
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr
225                 230                 235                 240 
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu
                245                 250                 255     
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys
            260                 265                 270         
Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser
        275                 280                 285             
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys
    290                 295                 300                 
Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile
305                 310                 315                 320 
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
                325                 330                 335     
Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu
            340                 345                 350         
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
        355                 360                 365             
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser
    370                 375                 380                 
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg
385                 390                 395                 400 
Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu
                405                 410                 415     
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            420                 425                 430     



