                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
       Guan, Shengxi
       Zhu, Zhenyu
       Blanchard, Aine
 
<120>  Restriction Endonuclease Variants with Altered Cleavage 
       Specificity and/or Reduction of Star Activity

<130>  NEB-317-PCT

<150>  61/239,875
<151>  2009-09-04

<160>  4     

<170>  PatentIn version 3.4

<210>  1
<211>  987
<212>  DNA
<213>  Bacillus thermoglucosidasius

<400>  1
atgcagatcg aacaattaat gaaaagtctc acaatctatt ttgacgacat acaagagggt     60

ttatggttta aaaacttgca tcctctatta gaatccgcgt ctcttgaggc gattactgga    120

tccctaaaaa gaaatccaaa cttagctgat gttttaaaat atgatagacc cgatatcatt    180

cttaccttga atcaaacacc catattagta atagagcgaa caattgaggt tccaagtggg    240

cataatgtcg gacaaagata tgggagatta gccgcagcat cggaagcagg agttccttta    300

gtctactttg gtccttacgc tgccagaaaa catggtgggg ctactgaagg accacgatac    360

atgaacttgc gtttatttta tgccctggat gtaatgcaaa aggtaaacgg ttctgctatt    420

accactataa attggcctgt agatcagaat ttcgaaatac tccaagatcc atctaaagat    480

aagagaatga aggagtattt agaaatgttc ttcgataatc ttttgaaata cggaatagcc    540

ggtataaatt tagcgattag aaattcctct tttcaagctg agcaattagc tgaaagagaa    600

aaatttgtgg aaactatgat aactaaccct gaacaatacg atgtcccgcc cgattcggtc    660

caaattctta atgctgaaag gttcttcaat gaattaggta tatcagaaaa taagagaata    720

atctgtgatg aggttgtttt atatcaagta ggaatgacat acgtcagatc agacccatat    780

actggaatgg cccttttata taagtatctt tatatacttg ggagcgaacg aaatagatgt    840

cttattttaa agttccctaa tattacaact gatatgtgga aaaaggtggc ttttggaagt    900

agagagcgga aagacgtaag aatctaccga agtgtctcag atggaatatt gtttgcagat    960

ggttatttat caaaagaaga gttgtaa                                        987


<210>  2
<211>  328
<212>  PRT
<213>  Bacillus thermoglucosidasius

<400>  2

Met Gln Ile Glu Gln Leu Met Lys Ser Leu Thr Ile Tyr Phe Asp Asp 
1               5                   10                  15      


Ile Gln Glu Gly Leu Trp Phe Lys Asn Leu His Pro Leu Leu Glu Ser 
            20                  25                  30          


Ala Ser Leu Glu Ala Ile Thr Gly Ser Leu Lys Arg Asn Pro Asn Leu 
        35                  40                  45              


Ala Asp Val Leu Lys Tyr Asp Arg Pro Asp Ile Ile Leu Thr Leu Asn 
    50                  55                  60                  


Gln Thr Pro Ile Leu Val Ile Glu Arg Thr Ile Glu Val Pro Ser Gly 
65                  70                  75                  80  


His Asn Val Gly Gln Arg Tyr Gly Arg Leu Ala Ala Ala Ser Glu Ala 
                85                  90                  95      


Gly Val Pro Leu Val Tyr Phe Gly Pro Tyr Ala Ala Arg Lys His Gly 
            100                 105                 110         


Gly Ala Thr Glu Gly Pro Arg Tyr Met Asn Leu Arg Leu Phe Tyr Ala 
        115                 120                 125             


Leu Asp Val Met Gln Lys Val Asn Gly Ser Ala Ile Thr Thr Ile Asn 
    130                 135                 140                 


Trp Pro Val Asp Gln Asn Phe Glu Ile Leu Gln Asp Pro Ser Lys Asp 
145                 150                 155                 160 


Lys Arg Met Lys Glu Tyr Leu Glu Met Phe Phe Asp Asn Leu Leu Lys 
                165                 170                 175     


Tyr Gly Ile Ala Gly Ile Asn Leu Ala Ile Arg Asn Ser Ser Phe Gln 
            180                 185                 190         


Ala Glu Gln Leu Ala Glu Arg Glu Lys Phe Val Glu Thr Met Ile Thr 
        195                 200                 205             


Asn Pro Glu Gln Tyr Asp Val Pro Pro Asp Ser Val Gln Ile Leu Asn 
    210                 215                 220                 


Ala Glu Arg Phe Phe Asn Glu Leu Gly Ile Ser Glu Asn Lys Arg Ile 
225                 230                 235                 240 


Ile Cys Asp Glu Val Val Leu Tyr Gln Val Gly Met Thr Tyr Val Arg 
                245                 250                 255     


Ser Asp Pro Tyr Thr Gly Met Ala Leu Leu Tyr Lys Tyr Leu Tyr Ile 
            260                 265                 270         


Leu Gly Ser Glu Arg Asn Arg Cys Leu Ile Leu Lys Phe Pro Asn Ile 
        275                 280                 285             


Thr Thr Asp Met Trp Lys Lys Val Ala Phe Gly Ser Arg Glu Arg Lys 
    290                 295                 300                 


Asp Val Arg Ile Tyr Arg Ser Val Ser Asp Gly Ile Leu Phe Ala Asp 
305                 310                 315                 320 


Gly Tyr Leu Ser Lys Glu Glu Leu 
                325             


<210>  3
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide


<220>
<221>  misc_feature
<222>  (12)..(14)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<400>  3
ggccccagtg annngtatca ttgcann                                         27


<210>  4
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide

<400>  4
ggccccagtg ctgcaatgat ac                                              22


