                         SEQUENCE LISTING

<110>  RWTH Aachen University
       Jacobs University Bremen
 
<120>  Directed evolution of arginine deiminase for increased activity 
       at physiological pH

<130>  R30344PCT

<150>  EP09011729.2
<151>  2009-09-14

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  417
<212>  PRT
<213>  Pseudomonas plecoglossicida

<400>  1

Met Ser Ala Glu Lys Gln Lys Tyr Gly Val His Ser Glu Ala Gly Lys 
1               5                   10                  15      


Leu Arg Lys Val Met Val Cys Ala Pro Gly Leu Ala His Lys Arg Leu 
            20                  25                  30          


Thr Pro Ser Asn Cys Asp Glu Leu Leu Phe Asp Asp Val Ile Trp Val 
        35                  40                  45              


Asp Gln Ala Lys Arg Asp His Phe Asp Phe Val Thr Lys Met Arg Glu 
    50                  55                  60                  


Arg Gly Val Asp Val Leu Glu Met His Asn Leu Leu Thr Asp Ile Val 
65                  70                  75                  80  


Gln Asn Pro Glu Ala Leu Lys Trp Ile Leu Asp Arg Lys Ile Thr Pro 
                85                  90                  95      


Asp Thr Val Gly Val Gly Leu Thr Asn Glu Val Arg Ser Trp Leu Glu 
            100                 105                 110         


Gly Gln Glu Pro Arg His Leu Ala Glu Phe Leu Ile Gly Gly Val Ala 
        115                 120                 125             


Gly Gln Asp Leu Pro Glu Ser Glu Gly Ala Ser Val Val Lys Met Tyr 
    130                 135                 140                 


Asn Asp Tyr Leu Gly His Ser Ser Phe Ile Leu Pro Pro Leu Pro Asn 
145                 150                 155                 160 


Thr Gln Phe Thr Arg Asp Thr Thr Cys Trp Ile Tyr Gly Gly Val Thr 
                165                 170                 175     


Leu Asn Pro Met Tyr Trp Pro Ala Arg Arg Gln Glu Thr Leu Leu Thr 
            180                 185                 190         


Thr Ala Ile Tyr Lys Phe His Pro Glu Phe Thr Lys Ala Asp Phe Gln 
        195                 200                 205             


Val Trp Tyr Gly Asp Pro Asp Gln Glu His Gly Gln Ala Thr Leu Glu 
    210                 215                 220                 


Gly Gly Asp Val Met Pro Ile Gly Lys Gly Ile Val Leu Ile Gly Met 
225                 230                 235                 240 


Gly Glu Arg Thr Ser Arg Gln Ala Ile Gly Gln Leu Ala Gln Asn Leu 
                245                 250                 255     


Phe Ala Lys Gly Ala Val Glu Gln Val Ile Val Ala Gly Leu Pro Lys 
            260                 265                 270         


Ser Arg Ala Ala Met His Leu Asp Thr Val Phe Ser Phe Cys Asp Arg 
        275                 280                 285             


Asp Leu Val Thr Val Phe Pro Glu Val Val Arg Glu Ile Val Pro Phe 
    290                 295                 300                 


Ile Ile Arg Pro Asp Glu Ser Lys Pro Tyr Gly Met Asp Val Arg Arg 
305                 310                 315                 320 


Glu Asn Lys Ser Phe Ile Glu Val Val Gly Glu Gln Leu Gly Val Lys 
                325                 330                 335     


Leu Arg Val Val Glu Thr Gly Gly Asn Ser Phe Ala Ala Glu Arg Glu 
            340                 345                 350         


Gln Trp Asp Asp Gly Asn Asn Val Val Ala Leu Glu Pro Gly Val Val 
        355                 360                 365             


Ile Gly Tyr Asp Arg Asn Thr Tyr Thr Asn Thr Leu Leu Arg Lys Ala 
    370                 375                 380                 


Gly Ile Glu Val Ile Thr Ile Ser Ala Gly Glu Leu Gly Arg Gly Arg 
385                 390                 395                 400 


Gly Gly Gly His Cys Met Thr Cys Pro Ile Val Arg Asp Pro Ile Asn 
                405                 410                 415     


Tyr 
    


<210>  2
<211>  1254
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  2
atgtccgctg aaaaacagaa gtacggtgtc cactccgaag caggcaagct gcgcaaggta       60

atggtctgcg ctccgggact ggcgcacaag cgcctgaccc cgagcaactg cgacgagctg      120

ctgttcgacg atgtgatctg ggtcgaccag gccaagcgcg accacttcga cttcgtcacc      180

aagatgcgcg agcgcggcgt ggatgtgctg gaaatgcata acctgctcac cgacatcgtg      240

cagaaccccg aggccctgaa gtggatcctc gaccgcaaga tcacccctga caccgtcggg      300

gtgggcctga ccaacgaagt gcgcagctgg ctggagggcc aggagccacg ccacctcgcc      360

gagttcctga tcggcggcgt ggccggccag gacctgccgg agagcgaagg tgccagcgtg      420

gtcaagatgt acaacgacta cctgggccac tccagcttca tcctgccgcc gctgcccaac      480

acccagttca cccgcgacac cacctgctgg atctacggcg gcgtgaccct caacccgatg      540

tactggccgg cgcgacgcca ggaaaccctg ctgaccaccg ccatctacaa gttccacccc      600

gagttcacca aggccgactt ccaggtctgg tacggcgacc cggaccaaga gcacggccag      660

gccaccctcg aaggcggcga cgtcatgccg atcggcaagg gcatcgtgct gatcggcatg      720

ggtgagcgca cctcgcgcca ggccatcggc caactggcac agaacctctt cgccaagggc      780

gcagtggagc aagtgatcgt cgccgggctg ccgaagtccc gtgcggccat gcacctggac      840

accgtgttca gcttctgcga ccgcgacctg gtcacggttt tcccggaagt ggtgcgcgag      900

atcgtgccgt tcatcatccg cccggacgaa agcaagccct acggcatgga cgtacgccgc      960

gagaacaagt cgttcatcga ggtggtcggc gagcagctgg gcgtcaagct gcgtgtggtc     1020

gagaccggcg gcaacagctt cgccgccgag cgcgagcagt gggatgacgg caacaacgtg     1080

gtggcgctgg agccaggtgt ggtcatcggc tacgaccgca acacctacac caataccttg     1140

ctgcgcaagg ccgggataga ggtcatcacc atcagtgccg gcgaactggg ccggggccgt     1200

ggcggcggcc actgcatgac ctgcccgatc gtgcgcgacc cgatcaacta ctaa           1254


<210>  3
<211>  29
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  3
ccgcattccg ctgaaaaaca gaagtacgg                                         29


<210>  4
<211>  34
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  4
cttctcgagt tagtagttga tcgggtcgcg cacg                                   34


<210>  5
<211>  33
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  5
ggccgtggcg gcggccgttg catgacctgc ccg                                    33


<210>  6
<211>  33
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  6
cgggcaggtc atgcaacggc cgccgccacg gcc                                    33


<210>  7
<211>  26
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  7
tacatatgtc cgctgaaaaa cagaag                                            26


<210>  8
<211>  24
<212>  DNA
<213>  Pseudomonas plecoglossicida

<400>  8
gtgctcgagt tagtagttga tcgg                                              24


