                         SEQUENCE LISTING

<110>  Naval Medical Research Center
       Casares, Sofia A.
       Thomas , Richie  L.
       Brumeanu, Teodor D.
 
<120>  Th1/Th2 polarizing vaccine

<130>  NC99154

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  2334
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of PyBTh1 heavy chain

<400>  1
gcactgaagc ttgtcctgat tgcctcagcc ttcagttttc aaatccagtc agtggtcatc       60

cctgaaaaca gacctttcac catgaagttg tggctgaact ggattttcct tctaacactt      120

ttaaaagata tccagtgtga ggtgaagctg ttggaatctg gaggaggttt ggtacagccg      180

gggggttctc tgagactctc ctgtgcagct tctggattca ccttcaatga tttctacatg      240

aactggatcc gccagcctcc agggcaggca cctgagtggt tgggtgttat tagaaacaaa      300

ggtaatggtt acacaacaga ggtcaataca tctgtgaagg ggcggttcac catctccaga      360

gataataccc aaaacatcct ctatcttcaa atgaacagcc tgagagctga ggacaccgcc      420

atttactact gtgcaagagg cggtccttat tactacagtg gtgacgacgc cccttactgg      480

ggccaaggag tcatggtcac agtctcctca gccaaaacaa cagccccatc ggtataccca      540

ctggcccctg tgtgtggaga tacaactggc tcctcggtga ctctaggatg cctggtcaag      600

ggttatttcc ctgagccagt gaccttgacc tggaactctg gatccctgtc cagtggtgtg      660

cacaccttcc cagctgtcct gcagtctgac ctctacaccc tcagcagctc agtgactgta      720

acctcgagca cctggcccag ccagtccatc acctgcaatg tggcccaccc ggcaagcagc      780

accaaggtgg acaagaaaat tgagcccaga gggcccacaa tcaagccctg tcctccatgc      840

aaatgcccag cacctaacct cttgggtgga ccatccgtct tcatcttccc tccaaagatc      900

aaggatgtac tcatgatctc cctgagcccc atagtcacat gtgtggtggt ggatgtgagc      960

gaggatgacc cagatgtcca gatcagctgg tttgtgaaca acgtggaagt acacacagct     1020

cagacacaaa cccatagaga ggattacaac agtactctcc gggtggtcag tgccctcccc     1080

atccagcacc aggactggat gagtggcaag gagttcaaat gcaaggtcaa caacaaagac     1140

ctcccagcgc ccatcgagag aaccatctca aaacccaaag ggtcagtaag agctccacag     1200

gtatatgtct tgcctccacc agaagaagag atgactaaga aacaggtcac tctgacctgc     1260

atggtcacag acttcatgcc tgaagacatt tacgtggagt ggaccaacaa cgggaaaaca     1320

gagctaaact acaagaacac tgaaccagtc ctggactctg atggttctta cttcatgtac     1380

agcaagctga gagtggaaaa gaagaactgg gtggaaagaa atagctactc ctgttcagtg     1440

gtccacgagg gtctgcacaa tcaccacacg actaagagct tctcccaaac tccgggtaaa     1500

ggaggtggtg gagggggagg cggtcaaggc ccaggggcac cacaggggcc tggtgcccca     1560

caaggaccgg gtgctcctca gggacccggg gcgccccaac agccgccaca gcaaccccca     1620

caacagcctc cgcaacaacc accgcagcag ccccctggag gtggtggatc cggtggaggg     1680

ggaagtggag gtggagggtc tgttgatgaa caactgtcca agtcagtgaa agataaggta     1740

ttgctgcctt gccgttacaa ctctcctcat gaagatgagt gtgaagaccg aatctactgg     1800

caaaaacatg acaaagtggt gctgtctgtc attgctggga aactaaaagt gtggcccgag     1860

tataagaacc ggactttata tgacaacact acctactctc ttatcatcct gggcctggtc     1920

ctttcagacc ggggcacata cagctgtgtc gttcaaaaga aggaaagagg aacgtatgaa     1980

gttaaacact tggctttagt aaagttgtcc atcaaagctg acttctctac ccccaacata     2040

actgagtctg gaaacccatc tgcagacact aaaaggatta cctgctttgc ttccgggggt     2100

ttcccaaagc ctcgcttctc ttggttggaa aatggaagag aattacctgg catcaatacg     2160

acaatttccc aggatcctga atctgaattg tacaccatta gtagccaact agatttcaat     2220

acgactcgca accacaccat taagtgtctc attaaatatg gagatgctca cgtgtcagag     2280

gacttcacct gggaaaaacc cccagaagac cctcctgata gcaagtgatc taga           2334


<210>  2
<211>  2178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of PyTh1 heavy chain

<400>  2
gcactgaagc ttgtcctgat tgcctcagcc ttcagttttc aaatccagtc agtggtcatc       60

cctgaaaaca gacctttcac catgaagttg tggctgaact ggattttcct tctaacactt      120

ttaaaagata tccagtgtga ggtgaagctg ttggaatctg gaggaggttt ggtacagccg      180

gggggttctc tgagactctc ctgtgcagct tctggattca ccttcaatga tttctacatg      240

aactggatcc gccagcctcc agggcaggca cctgagtggt tgggtgttat tagaaacaaa      300

ggtaatggtt acacaacaga ggtcaataca tctgtgaagg ggcggttcac catctccaga      360

gataataccc aaaacatcct ctatcttcaa atgaacagcc tgagagctga ggacaccgcc      420

atttactact gtgcaagagg cggtccttat tactacagtg gtgacgacgc cccttactgg      480

ggccaaggag tcatggtcac agtctcctca gccaaaacaa cagccccatc ggtataccca      540

ctggcccctg tgtgtggaga tacaactggc tcctcggtga ctctaggatg cctggtcaag      600

ggttatttcc ctgagccagt gaccttgacc tggaactctg gatccctgtc cagtggtgtg      660

cacaccttcc cagctgtcct gcagtctgac ctctacaccc tcagcagctc agtgactgta      720

acctcgagca cctggcccag ccagtccatc acctgcaatg tggcccaccc ggcaagcagc      780

accaaggtgg acaagaaaat tgagcccaga gggcccacaa tcaagccctg tcctccatgc      840

aaatgcccag cacctaacct cttgggtgga ccatccgtct tcatcttccc tccaaagatc      900

aaggatgtac tcatgatctc cctgagcccc atagtcacat gtgtggtggt ggatgtgagc      960

gaggatgacc cagatgtcca gatcagctgg tttgtgaaca acgtggaagt acacacagct     1020

cagacacaaa cccatagaga ggattacaac agtactctcc gggtggtcag tgccctcccc     1080

atccagcacc aggactggat gagtggcaag gagttcaaat gcaaggtcaa caacaaagac     1140

ctcccagcgc ccatcgagag aaccatctca aaacccaaag ggtcagtaag agctccacag     1200

gtatatgtct tgcctccacc agaagaagag atgactaaga aacaggtcac tctgacctgc     1260

atggtcacag acttcatgcc tgaagacatt tacgtggagt ggaccaacaa cgggaaaaca     1320

gagctaaact acaagaacac tgaaccagtc ctggactctg atggttctta cttcatgtac     1380

agcaagctga gagtggaaaa gaagaactgg gtggaaagaa atagctactc ctgttcagtg     1440

gtccacgagg gtctgcacaa tcaccacacg actaagagct tctcccaaac tccgggtaaa     1500

ggaggtggtg gatccggtgg agggggaagt ggaggtggag ggtctgttga tgaacaactg     1560

tccaagtcag tgaaagataa ggtattgctg ccttgccgtt acaactctcc tcatgaagat     1620

gagtgtgaag accgaatcta ctggcaaaaa catgacaaag tggtgctgtc tgtcattgct     1680

gggaaactaa aagtgtggcc cgagtataag aaccggactt tatatgacaa cactacctac     1740

tctcttatca tcctgggcct ggtcctttca gaccggggca catacagctg tgtcgttcaa     1800

aagaaggaaa gaggaacgta tgaagttaaa cacttggctt tagtaaagtt gtccatcaaa     1860

gctgacttct ctacccccaa cataactgag tctggaaacc catctgcaga cactaaaagg     1920

attacctgct ttgcttccgg gggtttccca aagcctcgct tctcttggtt ggaaaatgga     1980

agagaattac ctggcatcaa tacgacaatt tcccaggatc ctgaatctga attgtacacc     2040

attagtagcc aactagattt caatacgact cgcaaccaca ccattaagtg tctcattaaa     2100

tatggagatg ctcacgtgtc agaggacttc acctgggaaa aacccccaga agaccctcct     2160

gatagcaagt gatctaga                                                   2178


<210>  3
<211>  2070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of PyBTh2 heavy chain

<400>  3
gcactgaagc ttgtcctgat tgcctcagcc ttcagttttc aaatccagtc agtggtcatc       60

cctgaaaaca gacctttcac catgaagttg tggctgaact ggattttcct tctaacactt      120

ttaaaagata tccagtgtga ggtgaagctg ttggaatctg gaggaggttt ggtacagccg      180

gggggttctc tgagactctc ctgtgcagct tctggattca ccttcaatga tttctacatg      240

aactggatcc gccagcctcc agggcaggca cctgagtggt tgggtgttat tagaaacaaa      300

ggtaatggtt acacaacaga ggtcaataca tctgtgaagg ggcggttcac catctccaga      360

gataataccc aaaacatcct ctatcttcaa atgaacagcc tgagagctga ggacaccgcc      420

atttactact gtgcaagagg cggtccttat tactacagtg gtgacgacgc cccttactgg      480

ggccaaggag tcatggtcac agtctcctca gccaaaacaa cagccccatc ggtataccca      540

ctggcccctg tgtgtggaga tacaactggc tcctcggtga ctctaggatg cctggtcaag      600

ggttatttcc ctgagccagt gaccttgacc tggaactctg gatccctgtc cagtggtgtg      660

cacaccttcc cagctgtcct gcagtctgac ctctacaccc tcagcagctc agtgactgta      720

acctcgagca cctggcccag ccagtccatc acctgcaatg tggcccaccc ggcaagcagc      780

accaaggtgg acaagaaaat tgagcccaga gggcccacaa tcaagccctg tcctccatgc      840

aaatgcccag cacctaacct cttgggtgga ccatccgtct tcatcttccc tccaaagatc      900

aaggatgtac tcatgatctc cctgagcccc atagtcacat gtgtggtggt ggatgtgagc      960

gaggatgacc cagatgtcca gatcagctgg tttgtgaaca acgtggaagt acacacagct     1020

cagacacaaa cccatagaga ggattacaac agtactctcc gggtggtcag tgccctcccc     1080

atccagcacc aggactggat gagtggcaag gagttcaaat gcaaggtcaa caacaaagac     1140

ctcccagcgc ccatcgagag aaccatctca aaacccaaag ggtcagtaag agctccacag     1200

gtatatgtct tgcctccacc agaagaagag atgactaaga aacaggtcac tctgacctgc     1260

atggtcacag acttcatgcc tgaagacatt tacgtggagt ggaccaacaa cgggaaaaca     1320

gagctaaact acaagaacac tgaaccagtc ctggactctg atggttctta cttcatgtac     1380

agcaagctga gagtggaaaa gaagaactgg gtggaaagaa atagctactc ctgttcagtg     1440

gtccacgagg gtctgcacaa tcaccacacg actaagagct tctcccaaac tccgggtaaa     1500

ggaggtggtg gagggggagg cggtcaaggc ccaggggcac cacaggggcc tggtgcccca     1560

caaggaccgg gtgctcctca gggacccggg gcgccccaac agccgccaca gcaaccccca     1620

caacagcctc cgcaacaacc accgcagcag ccccctggag gtggtggatc cggtggaggg     1680

ggaagtggag gtggagggtc tcatatccac ggatgcgaca aaaatcactt gagagagatc     1740

atcggcattt tgaacgaggt cacaggagaa gggacgccat gcacggagat ggatgtgcca     1800

aacgtcctca cagcaacgaa gaacaccaca gagagtgagc tcgtctgtag ggcttccaag     1860

gtgcttctca tattttattt aaaacatggg aaaactccat gcttgaagaa gaactctagt     1920

gttctcatgg agctgcagag actctttcgg gcttttcgat gcctggattc atcgataagc     1980

tgcaccatga atgagtccaa gtccacatca ctgaaagact tcctggaaag cctaaagagc     2040

atcatgcaaa tggattactc gtagtctaga                                      2070


<210>  4
<211>  1914
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of PyTh2 heavy chain

<400>  4
gcactgaagc ttgtcctgat tgcctcagcc ttcagttttc aaatccagtc agtggtcatc       60

cctgaaaaca gacctttcac catgaagttg tggctgaact ggattttcct tctaacactt      120

ttaaaagata tccagtgtga ggtgaagctg ttggaatctg gaggaggttt ggtacagccg      180

gggggttctc tgagactctc ctgtgcagct tctggattca ccttcaatga tttctacatg      240

aactggatcc gccagcctcc agggcaggca cctgagtggt tgggtgttat tagaaacaaa      300

ggtaatggtt acacaacaga ggtcaataca tctgtgaagg ggcggttcac catctccaga      360

gataataccc aaaacatcct ctatcttcaa atgaacagcc tgagagctga ggacaccgcc      420

atttactact gtgcaagagg cggtccttat tactacagtg gtgacgacgc cccttactgg      480

ggccaaggag tcatggtcac agtctcctca gccaaaacaa cagccccatc ggtataccca      540

ctggcccctg tgtgtggaga tacaactggc tcctcggtga ctctaggatg cctggtcaag      600

ggttatttcc ctgagccagt gaccttgacc tggaactctg gatccctgtc cagtggtgtg      660

cacaccttcc cagctgtcct gcagtctgac ctctacaccc tcagcagctc agtgactgta      720

acctcgagca cctggcccag ccagtccatc acctgcaatg tggcccaccc ggcaagcagc      780

accaaggtgg acaagaaaat tgagcccaga gggcccacaa tcaagccctg tcctccatgc      840

aaatgcccag cacctaacct cttgggtgga ccatccgtct tcatcttccc tccaaagatc      900

aaggatgtac tcatgatctc cctgagcccc atagtcacat gtgtggtggt ggatgtgagc      960

gaggatgacc cagatgtcca gatcagctgg tttgtgaaca acgtggaagt acacacagct     1020

cagacacaaa cccatagaga ggattacaac agtactctcc gggtggtcag tgccctcccc     1080

atccagcacc aggactggat gagtggcaag gagttcaaat gcaaggtcaa caacaaagac     1140

ctcccagcgc ccatcgagag aaccatctca aaacccaaag ggtcagtaag agctccacag     1200

gtatatgtct tgcctccacc agaagaagag atgactaaga aacaggtcac tctgacctgc     1260

atggtcacag acttcatgcc tgaagacatt tacgtggagt ggaccaacaa cgggaaaaca     1320

gagctaaact acaagaacac tgaaccagtc ctggactctg atggttctta cttcatgtac     1380

agcaagctga gagtggaaaa gaagaactgg gtggaaagaa atagctactc ctgttcagtg     1440

gtccacgagg gtctgcacaa tcaccacacg actaagagct tctcccaaac tccgggtaaa     1500

ggaggtggtg gatccggtgg agggggaagt ggaggtggag ggtctcatat ccacggatgc     1560

gacaaaaatc acttgagaga gatcatcggc attttgaacg aggtcacagg agaagggacg     1620

ccatgcacgg agatggatgt gccaaacgtc ctcacagcaa cgaagaacac cacagagagt     1680

gagctcgtct gtagggcttc caaggtgctt ctcatatttt atttaaaaca tgggaaaact     1740

ccatgcttga agaagaactc tagtgttctc atggagctgc agagactctt tcgggctttt     1800

cgatgcctgg attcatcgat aagctgcacc atgaatgagt ccaagtccac atcactgaaa     1860

gacttcctgg aaagcctaaa gagcatcatg caaatggatt actcgtagtc taga           1914


<210>  5
<211>  849
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of the chimeric Ig-kappa

<400>  5
agaagttact ctcagtgaag atatagcatc aacatcagag ttctccctga gcttctgggg       60

ctgctgttac tctggttttc tggtgtgaga tgtgacatcc agatgacaca gtctccgtca      120

tttctgtcta catctcttgg aaacagcatc accatcactt gccatgccag tcagaacatc      180

aagggttggt tagcctggta ccaacaaaag tcagggaatg ctcctcaact gttgatttat      240

aaggcatcta gcctgcaatc aggggttcca tcaagattca gtggcagtgg atctggaaca      300

gattatattt tcactatcag caacctacag cctgaagata ttgccactta ttactgtcag      360

cattatcaaa gctttccgtg gacgttcggt ggaggcacca agctggaatt gaaacgggat      420

gctgcaccaa ctgtatccat cttcccacca tccagtgagc agttaacatc tggaggtgcc      480

tcagtcgtgt gcttcttgaa caacttctac cccaaagaca tcaatgtcaa gtggaagatt      540

gatggcagtg aacgacaaaa tggcgtcctg aacagttgga ctgatcagga cagcaaagac      600

agcacctaca gcatgagcag caccctcacg ttgaccaagg acgagtatga acgacataac      660

agctatacct gtgaggccac tcacaagaca tcaacttcac ccattgtcaa gagcttcaac      720

aggaatgagt gtggaggtgg tggatccggt ggagggggaa tgggaggtgg agggtcttac      780

aatcgaaata tagtcaacag attacttggc gatgctctca acggaaaacc agaagaaaaa      840

tgatctaga                                                              849


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Th1-Fg1-F primer

<400>  6
gcactgaagc ttgtcctgat tgcctcagcc ttc                                    33


<210>  7
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Th1-Fg1-F primer

<400>  7
cagtgggtat accgatgggg ctgttgtttt ggctgaggag actgtgacca t                51


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Th1-Fg2-F primer

<400>  8
ccatcggtat acccactggc ccctg                                             25


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ch1-R primer

<400>  9
gattgtgggc cctctgggct caattttc                                          28


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG2aFc-F primer

<400>  10
cagagggccc acaatcaagc cctgtcctcc a                                      31


<210>  11
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PyB-F primer

<400>  11
agggccccgg ggcgccccaa gagccgccac agcaaccccc acaacagcct ccgcaacaac       60

caccgcagca gccccctgga ggtggtggat ccggtggag                              99


<210>  12
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PyB-1R primer

<400>  12
cccctgtggt gcccctgggc cttgaccgcc tccccctcca ccacctcctt tacccggagt       60

ccgggag                                                                 67


<210>  13
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PyB-2R primer

<400>  13
gcgccccggg tccctgagga gcacccggtc cttgtggggc accaggcccc tgtggtgccc       60

ctgggccttg accgcctcc                                                    79


<210>  14
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B7.1-F primer

<400>  14
ggtggtggat ccggtggagg gggaagtgga ggtggagggt ctgttgatga acaactgtc        59


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B7.1-R primer

<400>  15
tgcatctaga tcacttgcta tcaggagggt c                                      31


<210>  16
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-4-F primer

<400>  16
ggtggtggat ccggtggagg gggaagtgga ggtggagggt ctcatatcca cggatgcgac       60


<210>  17
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-4-R primer

<400>  17
cctcctctag actacgagta atccatttgc atg                                    33


