                         SEQUENCE LISTING

<110>  INSERM
 
<120>  APTAMERS DIRECTED AGAINST THE MATRIX PROTEIN-1 OF TYPE A 
       INFLUENZA VIRUSES AND USES THEREOF

<130>  BIO09230 TOULME / MC

<160>  42    

<170>  PatentIn version 3.3

<210>  1
<211>  252
<212>  PRT
<213>  Influenza virus

<400>  1

Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Ile Pro 
1               5                   10                  15      


Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe 
            20                  25                  30          


Ala Gly Lys Asn Thr Asp Leu Glu Val Leu Met Glu Trp Leu Lys Thr 
        35                  40                  45              


Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 
    50                  55                  60                  


Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln His Arg Arg Phe Val 
65                  70                  75                  80  


Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Ile Asp Lys Ala 
                85                  90                  95      


Ala Lys Leu Tyr Arg Lys Leu Lys Arg Glu Val Thr Phe His Gly Ala 
            100                 105                 110         


Lys Glu Ile Ser Leu Ser Tyr Ser Ala Gly Ala Leu Ala Ser Cys Met 
        115                 120                 125             


Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Val Ala Phe 
    130                 135                 140                 


Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 
145                 150                 155                 160 


Ser His Gly Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 
                165                 170                 175     


Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 
            180                 185                 190         


Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Glu Val Ala Ser Gln 
        195                 200                 205             


Ala Arg Gln Met Val Gln Ala Met Arg Thr Ile Gly Thr His Pro Ser 
    210                 215                 220                 


Ser Ser Ala Gly Leu Lys Asn Asp Leu Leu Glu Asn Leu Gln Ala Tyr 
225                 230                 235                 240 


Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 
                245                 250         


<210>  2
<211>  121
<212>  PRT
<213>  Influenza virus

<400>  2

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Asp Ile Leu Leu Arg Met 
1               5                   10                  15      


Ser Lys Met Gln Leu Glu Ser Ser Ser Gly Asp Leu Asn Gly Met Ile 
            20                  25                  30          


Thr Gln Phe Glu Ser Leu Lys Leu Tyr Arg Asp Ser Leu Gly Glu Ala 
        35                  40                  45              


Val Met Arg Met Gly Asp Leu His Ser Leu Gln Asn Arg Asn Glu Lys 
    50                  55                  60                  


Trp Arg Glu Gln Leu Gly Gln Lys Phe Glu Glu Ile Arg Trp Leu Ile 
65                  70                  75                  80  


Glu Glu Val Arg His Lys Leu Lys Ile Thr Glu Asn Ser Phe Glu Gln 
                85                  90                  95      


Ile Thr Phe Met Gln Ala Leu His Leu Leu Leu Glu Val Glu Gln Glu 
            100                 105                 110         


Ile Arg Thr Phe Ser Phe Gln Leu Ile 
        115                 120     


<210>  3
<211>  498
<212>  PRT
<213>  Influenza virus

<400>  3

Met Ala Ser Gln Gly Thr Lys Arg Ser Tyr Glu Gln Met Glu Thr Asp 
1               5                   10                  15      


Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val Gly Lys Met 
            20                  25                  30          


Ile Gly Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr Glu Leu Lys 
        35                  40                  45              


Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu Thr Ile Glu 
    50                  55                  60                  


Arg Met Val Leu Ser Ala Phe Asp Glu Arg Arg Asn Lys Tyr Leu Glu 
65                  70                  75                  80  


Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly Gly Pro Ile 
                85                  90                  95      


Tyr Arg Arg Val Asn Gly Lys Trp Met Arg Glu Leu Ile Leu Tyr Asp 
            100                 105                 110         


Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn Gly Asp Asp 
        115                 120                 125             


Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser Asn Leu Asn 
    130                 135                 140                 


Asp Ala Thr Tyr Gln Arg Thr Arg Ala Leu Val Arg Thr Gly Met Asp 
145                 150                 155                 160 


Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro Arg Arg Ser 
                165                 170                 175     


Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met Val Met Glu 
            180                 185                 190         


Leu Val Arg Met Ile Lys Arg Gly Ile Asn Asp Arg Asn Phe Trp Arg 
        195                 200                 205             


Gly Glu Asn Gly Arg Lys Thr Arg Ile Ala Tyr Glu Arg Met Cys Asn 
    210                 215                 220                 


Ile Leu Lys Gly Lys Phe Gln Thr Ala Ala Gln Lys Ala Met Met Asp 
225                 230                 235                 240 


Gln Val Arg Glu Ser Arg Asn Pro Gly Asn Ala Glu Phe Glu Asp Leu 
                245                 250                 255     


Thr Phe Leu Ala Arg Ser Ala Leu Ile Leu Arg Gly Ser Val Ala His 
            260                 265                 270         


Lys Ser Cys Leu Pro Ala Cys Val Tyr Gly Pro Ala Val Ala Ser Gly 
        275                 280                 285             


Tyr Asp Phe Glu Arg Glu Gly Tyr Ser Leu Val Gly Ile Asp Pro Phe 
    290                 295                 300                 


Arg Leu Leu Gln Asn Ser Gln Val Tyr Ser Leu Ile Arg Pro Asn Glu 
305                 310                 315                 320 


Asn Pro Ala His Lys Ser Gln Leu Val Trp Met Ala Cys His Ser Ala 
                325                 330                 335     


Ala Phe Glu Asp Leu Arg Val Leu Ser Phe Ile Lys Gly Thr Lys Val 
            340                 345                 350         


Leu Pro Arg Gly Lys Leu Ser Thr Arg Gly Val Gln Ile Ala Ser Asn 
        355                 360                 365             


Glu Asn Met Glu Thr Met Glu Ser Ser Thr Leu Glu Leu Arg Ser Arg 
    370                 375                 380                 


Tyr Trp Ala Ile Arg Thr Arg Ser Gly Gly Asn Thr Asn Gln Gln Arg 
385                 390                 395                 400 


Ala Ser Ala Gly Gln Ile Ser Ile Gln Pro Thr Phe Ser Val Gln Arg 
                405                 410                 415     


Asn Leu Pro Phe Asp Arg Thr Thr Ile Met Ala Ala Phe Asn Gly Asn 
            420                 425                 430         


Thr Glu Gly Arg Thr Ser Asp Met Arg Thr Glu Ile Ile Arg Met Met 
        435                 440                 445             


Glu Ser Ala Arg Pro Glu Asp Val Ser Phe Gln Gly Arg Gly Val Phe 
    450                 455                 460                 


Glu Leu Ser Asp Glu Lys Ala Ala Ser Pro Ile Val Pro Ser Phe Asp 
465                 470                 475                 480 


Met Ser Asn Glu Gly Ser Tyr Phe Phe Gly Asp Asn Ala Glu Glu Tyr 
                485                 490                 495     


Asp Asn 
        


<210>  4
<211>  68
<212>  RNA
<213>  Artificial

<220>
<223>  aptamer M1R9C1

<400>  4
gguuaccagc cuucacugcc ugaccacuca gaaucgagcg cauuggccgg caccacgguc     60

ggucacac                                                              68


<210>  5
<211>  68
<212>  RNA
<213>  Artificial

<220>
<223>  aptamer M1R9C6

<400>  5
gguuaccagc cuucacugcc cugaccaucc ugagggacgc auuggccggg caccacgguc     60

ggucacac                                                              68


<210>  6
<211>  68
<212>  RNA
<213>  Artificial

<220>
<223>  aptamer M1R9C9

<400>  6
gguuaccagc cuucacugcc ugaccauccu auuccggacg cauuggccgg caccacgguc     60

ggucacac                                                              68


<210>  7
<211>  68
<212>  RNA
<213>  Artificial

<220>
<223>  aptamer M1R9C20

<400>  7
gguuaccagc cuucacugcc cugaccaccu agauaggcgc auuggccggg caccacgguc     60

ggucacac                                                              68


<210>  8
<211>  36
<212>  RNA
<213>  Artificial

<220>
<223>  aptamer M1R9C1 36 bases length

<400>  8
ugccugacca cucagaaucg agcgcauugg ccggca                               36


<210>  9
<211>  36
<212>  RNA
<213>  Artificial

<220>
<223>  aptamer M1R9C6 36 bases lenght

<400>  9
ugcccugacc auccugaggg acgcauuggc cgggca                               36


<210>  10
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  aptamer M1R9C1 ARN/DNA

<400>  10
ugccugacca cucagaatcg agcgcauugg ccggca                               36


<210>  11
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  aptamer M1R9C6 ARN/DNA

<400>  11
ugcccugacc aucctgaggg acgcauuggc cgggca                               36


<210>  12
<211>  6
<212>  RNA
<213>  Artificial

<220>
<223>  NS7

<400>  12
agaauc                                                                 6


<210>  13
<211>  4
<212>  RNA
<213>  Artificial

<220>
<223>  NS7

<400>  13
ugag                                                                   4


<210>  14
<211>  6
<212>  RNA
<213>  Artificial

<220>
<223>  NS7

<400>  14
uauucc                                                                 6


<210>  15
<211>  4
<212>  RNA
<213>  Artificial

<220>
<223>  NS7

<400>  15
agau                                                                   4


<210>  16
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  NS7

<400>  16
agaatc                                                                 6


<210>  17
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  NS7

<400>  17
tgag                                                                   4


<210>  18
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS1

<400>  18
gcc                                                                    3


<210>  19
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS2

<400>  19
ggc                                                                    3


<210>  20
<211>  4
<212>  RNA
<213>  Artificial

<220>
<223>  NS1

<400>  20
gccc                                                                   4


<210>  21
<211>  4
<212>  RNA
<213>  Artificial

<220>
<223>  NS2

<400>  21
gggc                                                                   4


<210>  22
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS3

<400>  22
cca                                                                    3


<210>  23
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS4

<400>  23
ugg                                                                    3


<210>  24
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS5

<400>  24
cuc                                                                    3


<210>  25
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS6

<400>  25
gag                                                                    3


<210>  26
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS5

<400>  26
ucc                                                                    3


<210>  27
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS6

<400>  27
ucc                                                                    3


<210>  28
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS5

<400>  28
ccu                                                                    3


<210>  29
<211>  3
<212>  RNA
<213>  Artificial

<220>
<223>  NS6

<400>  29
agg                                                                    3


<210>  30
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  ssDNA


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  randomized sequence

<400>  30
gtgtgaccga ccgtggtgcn gcagtgaagg ctggtaacc                            39


<210>  31
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer p20

<400>  31
gtgtgaccga ccgtggtgc                                                  19


<210>  32
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  32
taatacgact cactataggt taccagcctt cactgc                               36


<210>  33
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  probe

<400>  33
tgccggccaa                                                            10


<210>  34
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  probe

<400>  34
tgcccggcca                                                            10


<210>  35
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  aptamer M1R9C1 26 bases-length

<400>  35
ugccugacca cucagaaucg agcgca                                          26


<210>  36
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  M1R9C1 RNA/DNA aptamer 26 bases-length

<400>  36
ugccugacca cucagaatcg agcgca                                          26


<210>  37
<211>  26
<212>  RNA
<213>  Artificial

<220>
<223>  M1R9C6 aptamer 26 bases-length

<400>  37
ugcccugacc auccugaggg acgcau                                          26


<210>  38
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  M1R9C6 RNA/DNA aptamer 26 bases-length

<400>  38
ugcccugacc aucctgaggg acgcau                                          26


<210>  39
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  M1R9C1 DNA

<400>  39
tgcctgacca ctcagaatcg agcgcattgg ccggca                               36


<210>  40
<211>  31
<212>  RNA
<213>  Artificial

<220>
<223>  anti-VEGF

<400>  40
ggcgaaccga uggaauuuuu ggacgcucgc c                                    31


<210>  41
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  anti-PDGF

<400>  41
caggctacgg cacgtagagc atcaccatga tcct                                 34


<210>  42
<211>  37
<212>  RNA
<213>  Artificial

<220>
<223>  MIR9C6 bulge less

<400>  42
ugcccuggcc auccugaggg acgcauuggc cagggca                              37


