                         SEQUENCE LISTING

<110>  Dow AgroSciences LLC
       Ainley, William Michael
       Blue, Ryan C.
       Murray, Michael G.
       Corbin, David Richard
       Miles, Rebecca Ruth
       Webb, Steven R.
 
<120>  ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS

<130>  2971-9880.1PC (69559)

<150>  US 61/297,641
<151>  2010-01-22

<160>  38    

<170>  PatentIn version 3.4

<210>  1
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  1
ttcatctttg gacaagggaa taaagactcc ccacttgcta ctaagaacaa tacctaagtt     60

gcccagacat gactgtaccc attcagagac ctaccaccca ttagggctat gacactaaca    120

ctagcccctg gaggttgacc atgctaggca gtgggggtct cacctatgac ccactcagat    180

aggggattgg tccagtgggt gggatctcag cctcatatag gtgtttgtgg tgagctttct    240

cctagacaag agaaccctga agaacagcaa gaaccagcta atatgatatg tagacatagt    300

gggttgctca aattttgtgt ttagtcatat tagaattgac ctcagtgacc actcagaaag    360

tgcccaagcc catctatagg ggccaaagtg ctattgactg gtgtgtctgt gaattgttcc    420

tccctacaga gttggtgctg atatatccta gcattctttg gaaaacctag ctagggactg    480

tcaagtgtaa gatacctcct gaattggagg gaacactagc tgccctgtac cttctggcta    540

gtaccttaca ccctgaatgg gttagggggt ctattatttg ctggaaatat accagtttca    600

gtagggctgc tgccttaggt cccacaaggt gtaacatgtg ctcaatagtt gcactaccac    660

atgcacgtga acttaatgat gttatagcca caacaccaac cttggtttgc agtttgacat    720

ccctctggaa tgggtgtagt catcttgctc tggatctgcc tgaatcattg gggctgtatg    780

cagcctgggc ttaaagtgaa gaatgggatg tcccagaaat attttgggtg agaagaatcc    840

tggagtagat ggtgacctga ctatccctgt cctatgggca caatctatca tcagatattg    900

cattcaaagg gctatcatgg gatcaagtcc taagtcaact gttgtttacc tggcagacat    960

tcatctagga gttctctttt atgccacccc acagtgatcc                         1000


<210>  2
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  2
taaaatatag agtatagggg ttatcatcac agagaagcta ttgctggagg gcctctgtta     60

tttcctctcc atgccactcc catttttaac ctaccaactg aaatcccaag ggagactcca    120

ccctgtaact agagtcctca gaggtgagcc atcccatatt aacaaatggg cattagggct    180

aggatgccaa gggatacctg aaatgggaag ttgtggggct gagtcctcct gggaatcaga    240

gataatatgt aaacagtttg ttgagagatt gatgagagct gactttgaga ggtggccatg    300

ctccctggtc ctcaataggg aaggcactac acaagaaacc tgggtttgat caactgcact    360

gtgtcctact cacacattgt gtgcctggaa aaatgttact tagtatttgg agggcctcca    420

gaacccccct gggtgcaaga ctgggtgcta gtgactgggt gaatgagtct tggacacagt    480

ggccttgtct aggttgtgtg aggtggctag gcatcatggc aatacctcat aattgatgag    540

tgaggaaaca agactaagtc cttgactcct cttattacat gacctggtgg atattatgct    600

ctcaatctgc aagctggaat gagtactggg tgcagatccc ctgggattct ggctacaaag    660

gtgaatgata gctagtctgt ttattagtag ccaaaaaagt cagtgagggg tgagtgccct    720

gggatgttgt taagttcaca ttgcacactt ggagaccctc tccatccagt aacataccag    780

agaaaactga ccaagccctc atgggtgtat gggaacaaca aacctcctgg ctacttcaag    840

ggcacataac accagcaagg agcctgtcat aaccaccatc tcaaacaata gaacttccta    900

agtgaagcaa tgacttcaaa tctacttgaa ggcatggagt ataagccatg ttcctttcag    960

aggggactgt acttctgtag attactttcc ctcattaacc                         1000


<210>  3
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  3
tgcactcatg ttcatatcca tcacacccac tgaggcagat attgcagtga gagggttgca     60

tgtagggtcc tgctacctgg ccatgtaggt tttgggctgg gaaacaagga aaataatcca    120

aggtcccacc acacacaaga gcttctaaag gtcatggtgt gctggatgag aaggccatta    180

agaatctgga cctgttagag gaaccagtac cttacaaggg gatggtccac cctttaatca    240

tcagcttgac caaccccttt agtattcacc tctggcagac accagctttg ggattcctat    300

ctaaccattt ggccagtacc tggcactcac accagtggag actacagagc tacatgaatg    360

ggtcagatcc ccactccata gtgcatagct ggccacatga tactttaccc tagatatttg    420

ccataccgga gagtctgttc cctggtttcg accgggagtg ggccaccttg tctttggggc    480

ctaagctgtg gagtgatggt tctggcacca cctgtgggtc tgatagcaca tgccccctcc    540

tgcttatcca gtggtctgtt ctcatggata ttcaaggctt gtatagatgg aaaaggatga    600

ggtgggtgaa ggctgttggg aagtgtgcct atgaagcccc aacaaagaga ttagacagta    660

cagctgatta cctaactcct tgggatgtct caaacaaatg tttcaaggcc cttttccctt    720

ggagcatgaa cctggcaata taaagtattt catctaaggg tcttatacaa attacccttt    780

tacaccatta tggaccctga ggcaagctgt ctggcccact ctagctacct ggcaaatgtc    840

cacctgtagc tcataccaat ctttaagctc agagcaaagt tcagaagccc ttcagtgaca    900

gtggcaatgg ctatccagtg aacctgaccc aattagtggc ccattggatt attgaaaact    960

ggcagtgctg agtccatcac cccaaatact gtccataggc                         1000


<210>  4
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  4
tcagaccacc tcaccaccac aaagccttgc cctgaccacc ttgtaggctg gcctagacaa     60

tggtcatctt tggtgtcctc ctacccatta gagagagcca ctttcatacc agcatccagt    120

acctgaggaa taatggcctc aatggcatcc acaagtctca ctgggttggc ctgaggagca    180

gcccatgcct gtgccctagt ttaaagagga gtgatggtgc ttcctgcctc ttgagtggaa    240

ccccttgttc catagtgaga atgggaggtg tgggccattc tgacaaagga ttttggacaa    300

gtccagctat taacacctgg ctagggatta acacagtgat acctggttga aggtgggagg    360

gcaagtgatc taacaaatct gttttgcaaa ggacatcaga aatctgaggt gcctatagag    420

aacatcccca aggtttagca gaaagaactc tttaggctga ctcctggata atctttccaa    480

ggtggagcca ttaggcacac aactgggata cttgtcagta gggattaaag acctttataa    540

gtctcaaggt ctgggtgtta aaggacagag tttattccaa gagggaatag agcaaaggca    600

tatatgggag tgtgggactg atccctgacc tctataatgg gagaggacat taggacctaa    660

ggtgcagaat agtggccttc cttagcctct ggaagtaatg gtgggattcc ccttaacata    720

ccctcctaaa aactgagaag tgggggaccc tggggtggga tcaaaagatt agggactggt    780

gtgtaaggtg aacatgtgct gcaaggatca tcctggctgt ggttgcctca tcagctagag    840

aaagcccctg ccactaagcc atctgcaatg cctaatcttg gaacaaggaa gctgggactg    900

atccattatt gtcaaaacaa ctggtgaggg tgtgccctaa ggttggtgtt tactgattgt    960

ggtcttgggt ctagggactt ccagatatgg aaaaaattag                         1000


<210>  5
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  5
tgtaccaatg ttgtctccct taactaggga atatctggcc agccctcatg tataggtgtt     60

ttagtacact ctggtgagtg cattgctcag tcaactggtg gaacctgtcc ccccatccta    120

agggcaaccc cttgtaccca atttgaagat agggttgcta gagcagtgca atgaggtccc    180

ttgagtaagc agatggcctg ctaggcttca aacctcagac ctggtttttt gtatggcctg    240

ggatcagttg attatctacc ccatcatcaa taacttgccc ttaatactta aacatagggc    300

caatcattgt ggggcctcta gggaaactgt ttggtcctgg cactgtttct aaaaggggaa    360

cacatcataa tcagtaggaa acagaaaagc aagagtggag cctaccctag ttgcccagtg    420

tgggagcaaa attgatgtaa ggctacatag aacccctata ccctagtagg tttacctgat    480

cattaggctg gctccccctg tcccatagag cacactcaat ctcatggcca agacttgaag    540

gttaaaatct catggggctg ctgatcagat tgcccacaaa aggtgttacc tgatacagtc    600

taacttctta cctgccagca aaaattttga tggaagtatc ctggcaactg taatcctcta    660

ccacttaaag attctagtat tgattaactt ctctccatac ctaaacagca gaatgtagca    720

taggttcata ggcttccttt ggaggttcac tatggaactt accactggat gacaaccctc    780

acctgtgcta ctctccctac cttggaggac cattgttgac ctagagactt atctgagtgc    840

taacccatga cattaacctc tgggggcttc cccatccctg gacatgtggc tggagaaaat    900

ataggtcaaa aacattgagc acattaggtt gccaatggtg aggctctcta agggtctggg    960

ttacaccaga ttagcagttc ttgacatggg agagtgtgat                         1000


<210>  6
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  6
tcgtctcacg cttgctgaat ctgatcagac tattaggccc ctcatcttga acatctttgc     60

catcctttta gcttggttgt cagtggaaac tagggtccaa tggtttagag tccagccaga    120

tggaggttgg gcacaatgcc cctgcactat ccaccctagc tgtagaagtt atgcttgtcc    180

aaaggttatg caactgctgt ctgttgggct tgagccaaga gattggtggg aaatcttgcc    240

agagttggcc agttgtctcc tgtccaccaa catgatccta tatatgggtc cccaccctgg    300

ttcccaagcc actcttgtag gctgaagagg aaacacctca gaagctgagt agtccaagac    360

tgcttgcacc aagtttccac cccttacctg tcctgaaatt aaaggcacca gtgttccggt    420

tcccttggac tttctacttg tagtgtagca ctgggtggac ctactccctt tatcctggga    480

tcatggcact ttctattaca gtgggtcaca acttgaggca gccctgtagt tgagtatata    540

gtggagagca ccagatgatg attttacttg gtgtaggttg tccctggccc ttagctgtgg    600

tgaccccatt gtcatcctcc agttccaact ttccatttcc tgcaatgagt ggtccacatt    660

gctggctggg ggtggatcta tccctccatt tgggaagaaa cagtgatatg gacactaacc    720

accataagtg gatggattct tcatagagtg gttgcttttg cccaagtctg tgcagcagag    780

gtgtcaacct ggtccccaat tagtgaaata atctcacagc ctgggggcta ctcgtattag    840

gaccagtacc cattccagcc tggcccaaaa ctttagttga gcctagacct tcccacttac    900

atggactgat tgtggcggtt ccagatgact gttgacttcc tatccatttg gacttttgga    960

ggggccagaa acctattcct ccagtatagt cctccacatt                         1000


<210>  7
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  7
tgtcacccag aggttccctc tgtacagctg aaaatccatt agtaaagcca tataactccc     60

acccagaggg tggcagtatc ctatctcaat accactgctg ggtgatagct tctcatccct    120

tattactgag gcccaaggct atttgaccaa tagagctggt ctcccccact tcatcaaccc    180

tcactattaa ccctgtatct actagccact tgaaaccagt gccattctta aaatgtagga    240

tggttttagc catagagaac tggcatagtg gagggagtgt aggggcctct gcctggtggg    300

aaggaaccct gattcagagc tttaagcctg tagggcctta aaagtacctt gatgacttgg    360

gtccctaaag tcccttgggc caatgctgat ggatacctct tggtccccaa aggattccct    420

tgaattacat ctgataggga ctccattgga cagcagcaca aaagtctgat ctaaactcac    480

ctgagatagt tactctgcat ccattgctgg ccattacaag tccatcacaa tgtggaaggt    540

tttggagggt gaacagagcc aaggacagct taatgacttt tcccaaggag ggctaggcac    600

tcacaagtgt tgtttgtaca ccatctggct atggcaaaac ccaaaacact ttatgcctgg    660

tggaaaagaa gtggccatga tactttgttg atgggcagtt caagaaagag tgggtgatat    720

gaccctcttg ttaggcagag tttcaaggat tacctcctcc agcagatggg accatgttcc    780

ctcaaggtga gtagggccta aatcatgcct ggagttggag tttgtagggt ctatgagaca    840

ttttcatact gtagaaacat cctattgtag gaggcaagcc acaagtggat tgtttaaagt    900

ggtgtcagtt gaattgaata gaactccatg ttagagggtc cctagtgttg tccctgcatg    960

tattttagtg ccatccatct agtctataat atcacctagt                         1000


<210>  8
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  8
ttccaccctg acagagttaa tcctagctta cccaagggca gtaacatgca gtggatggca     60

gtagggcaca actctgctgt ttatccacct ttgagtgtct ggcatcctag cataactggc    120

tacatgagag ggttaatggt ccctggcagc cctggtggtt gcagcctgtc ctggctccaa    180

gttaataccc ctatattggg atctattgga cttgctgggc actgcctgat ggttcaccag    240

ctatgtcaca tggttgggag tggacaccca agtgatacct cctaagtgag aggagcaaat    300

tacaatgcca ctttatgggc cactgggtgc atttgaggag cctagtgttg ctttttcttg    360

ggacccattg gtgctgggaa agggattcca cacagtggga gggtgcctta aactcctcat    420

gtgggggttg gagctggaac taggagtggc tgactacaag cctggcttat tgacatcatt    480

agtggtgaat ggtttccaga gcttgacaaa gctgaactgt taggaggacc tgggaccaat    540

cagagataat cttctaactg gcattggtgc tattaagtaa accatctcac cagtcagcca    600

aggggttctt tcactgtcta cttgtgggac cttggccaca ctggtggacc catctattgg    660

tgttcaactg atcctgacat ttctcagctt aacagatgga cctacttggc accacattgg    720

ggggcttctt gacagttaag gtgacacctt agttggctga catgtaaact ccagtgatcc    780

ttgggcactt gtgattccca ccttactgtc cacattgcca ccagtttgct accaaggctt    840

tactgtccca taaggttgtg gcctaacctt tgggctgcaa attaacatga atatccatag    900

aagatcaaga acctagtcac ttctgtagtg agtatggcag tgataagcag cttgactcct    960

agatggcagt tgggcttatg gaggatctac tgcctacagt                         1000


<210>  9
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  9
tgcttgactc ttgcatagtg tacttccacc cacacactca aaccttaata taggccatcc     60

ctgaggcaag aactccagtg gcacttattc tatgatccca aactaggcaa gggactagca    120

caagccacca gcactggcta accctgaagg caaggatatt acatccagac acctcggacg    180

tagcacacct cttactgttc taatggactt aatctacgat ctcacgatca tgggggggtc    240

ttttggagtc ctacaatcct cttagaacaa cctatgggca tatatccatt cttctctcaa    300

cctgaggaga ctgggccaca acttccttat agggcattgt gagacctgct atgagggagt    360

agctggtggg ttggttccct ggcagtttag tagggccaaa tagcagatac gtcctttcat    420

ggaagtggga tatagggaag agaaatgtag cattggagca tgttaaacta aaaagacttt    480

ttccagcatc ttgacccaaa agattacagt atttgaacta agtgtggagc accaaaaaac    540

aaccagtacc tcttacaatt ccccactctt tctgctactg tgccttatgc cctgcgttga    600

aagatattgc cattctgctc actgattatt ttggtcagct ggattggcat gggaaaacaa    660

tcagtgtcca ccttacagag gccatgtaga tagtcactat attactcagc tcatagacct    720

gttaatcaag tttatctggt cactagggac tgcaaacaca atgcccatca ttcccacgct    780

agtgtaaacc tcactatttc tgtcaaaccc tgacatcatg ctagaggagc aacactgcct    840

ggatggtatc agaagaggat cgcccaagcc cccttccact gacttctgga gttgtgaagg    900

caacagctga taatggaagt cacctccttg accacgctac taaatgctta tcaattcacc    960

tacccttgga ttcaaggagc acttaccaaa gcccagaccc                         1000


<210>  10
<211>  1001
<212>  DNA
<213>  Artificial

<220>
<223>  Example sequence flanking at least one targeting endonuclease 
       binding site

<400>  10
tttcataggg atatgtgagg actaaccttg gccaaaggag ctggaactgc ttatgtaagg     60

gccttagtcc aaattgctcc accctctggg aagctaatgg tccctccttg ggccttatag    120

tgaagcggaa tgggcatacc tagttgtctg ttctgggttg accctgctgg actcctcagt    180

tttagttcca ctaccaaaga ccttgagtct acagatgtgt tgctaaatcc tcacaaacct    240

ccaacaattt ttatgccctc tatcttctcc aaagttgctg ctgatccctg ccctcttatt    300

aagagccata gcaacaatac ctgaggtgag ggtttcacca ccaccatcta cctggaaaca    360

tcagccctcc tacatcttac cactcccaat agggggctta gccactcttg aggtggtcat    420

cctttagcac ctttggttct aggataggct gggcaccctg gcagatgccc actgacagcc    480

ctagaggaac ccaaggggaa acaagccact acatacaact tggcaagcaa gtcaaggcaa    540

ggcttgacta atctatagat caaggttagt accacttaat tggggaagtc cagaaagtga    600

gcactaagta tgagcaaccc tctaatgact cagactaaag ggtgacaagc caagtaaatg    660

ttttctacat tagaggttcc tgggggcttg atttctactt ccttaggatt ccctggtctt    720

agggagaata tgtcctctta ggtgtcctag ccaaggacca gtaacaattc ttcctggacc    780

tctattttgc tttataccac actcaatcac tttgggattc attccatgcc tcaagagaca    840

ccaagataga gggaaattgg ggacccaagt ggggagcatt ttttgtctct ccttgagggc    900

aactctggac agtgaggaac taggagggtg tgatctctat atagatggag tgttgtacct    960

aagggtgaaa gctggcacat ccattggttc aagtttgccc t                       1001


<210>  11
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Modified example sequence flanking at least one targeting 
       endonuclease binding site

<400>  11
ttcatctttg gacaagggaa taaagactcc ccacttgcta ctaagaacaa tacctaagtt     60

gcccagacat gactgtaccc attcagagac ctaccaccca ttagggctat gacactaaca    120

ctagcccctg gaggttgacc atgctaggca gtgggggtct cacctatgac ccactcagat    180

aggggtttaa accagtgggt gggatctcag cctcatatag gtgtttgtgg tgagctttct    240

cctagacaag agaaccctga agaacagcaa gaaccagcta atatgatatg tagacatagt    300

gggttgctca aattttgtgt ttagtcatat tagaattgac ctcagtgacc actcagaaag    360

tgcccaagcc catctatagg ggccaaagtg ctattgactg gtgtgtctgt gaattgttcc    420

tccctacaga gttggtgctg atatatccta gcattctttg gaaaacctag ctagggactg    480

tcaagtgtaa gatacctcct gaattggagg gaacactagc tgccctgtac cttctggcta    540

gtaccttaca ccctgaatgg gttagggggt ctattatttg ctggaaatat accagtttca    600

gtagggctgc tgccttaggt cccacaaggt gtaacatgtg ctcaatagtt gcactaccac    660

atgcacgtga acttaatgat gttatagcca caacaccaac cttggtttgc agtttgacat    720

ccctctggaa tgggtgtagt catcttgctc tggatctgcc tgaatcattg gggctgtatg    780

cagcctgggc ttaaagtgaa gaatgggatg tcccagaaat attttgggtg agaagaatcc    840

tggagtagat ggtgacctga ctatccctgt cctatgggca caatctatca tcagatattg    900

cattcaaagg gctatcatgg gatcaagtcc taagtcaact gttgtttacc tggcagacat    960

tcatctagga gttctctttt atgccacccc acagtgatcc                         1000


<210>  12
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Modified example sequence flanking at least one targeting 
       endonuclease binding site

<400>  12
tgcactcatg ttcatatcca tcacacccac tgaggcagat attgcagtga gagggttgca     60

tgtagggtcc tgctacctgg ccatgtaggt tttgggctgg gaaacaagga aaataatcca    120

aggtcccacc acacacaaga gcttctaaag gtcatggtgt gctggatgag aaggccatta    180

agtttaaaca cctgttagag gaaccagtac cttacaaggg gatggtccac cctttaatca    240

tcagcttgac caaccccttt agtattcacc tctggcagac accagctttg ggattcctat    300

ctaaccattt ggccagtacc tggcactcac accagtggag actacagagc tacatgaatg    360

ggtcagatcc ccactccata gtgcatagct ggccacatga tactttaccc tagatatttg    420

ccataccgga gagtctgttc cctggtttcg accgggagtg ggccaccttg tctttggggc    480

ctaagctgtg gagtgatggt tctggcacca cctgtgggtc tgatagcaca tgccccctcc    540

tgcttatcca gtggtctgtt ctcatggata ttcaaggctt gtatagatgg aaaaggatga    600

ggtgggtgaa ggctgttggg aagtgtgcct atgaagcccc aacaaagaga ttagacagta    660

cagctgatta cctaactcct tgggatgtct caaacaaatg tttcaaggcc cttttccctt    720

ggagcatgaa cctggcaata taaagtattt catctaaggg tcttatacaa attacccttt    780

tacaccatta tggaccctga ggcaagctgt ctggcccact ctagctacct ggcaaatgtc    840

cacctgtagc tcataccaat ctttaagctc agagcaaagt tcagaagccc ttcagtgaca    900

gtggcaatgg ctatccagtg aacctgaccc aattagtggc ccattggatt attgaaaact    960

ggcagtgctg agtccatcac cccaaatact gtccataggc                         1000


<210>  13
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Modified example sequence flanking at least one targeting 
       endonuclease binding site

<400>  13
taaaatatag agtatagggg ttatcatcac agagaagcta ttgctggagg gcctctgtta     60

tttcctctcc atgccactcc catttttaac ctaccaactg aaatcccaag ggagactcca    120

ccctgtaact agagtcctca gaggtgagcc atcccatatt aacaaatggg cattagggct    180

aggatgccaa gggatacctg aaatgggaag ttgtggggct gagtcctcct gggaatcaga    240

gataatatgt aaacagtttg ttgagagatt gatgagagct gactttgaga ggtggccatg    300

ctccctggtc ctcaataggg aaggcactac acaagaaacc tgggtttgat caactgcact    360

gtgtcctact cacacattgt gtgcctggaa aaatgttact tagtatttgg agggcctcca    420

gaacccccct gggtgcaaga ctgggtgcta gtgactgggt gaatgagtct tggacacagt    480

ggccttgtct aggttgtgtg aggtggctag gcatcatggc aatacctcat aattgatgag    540

tgaggaaaca agactaagtc cttgactcct cttattacat gacctggtgg atattatgtt    600

taaactctgc aagctggaat gagtactggg tgcagatccc ctgggattct ggctacaaag    660

gtgaatgata gctagtctgt ttattagtag ccaaaaaagt cagtgagggg tgagtgccct    720

gggatgttgt taagttcaca ttgcacactt ggagaccctc tccatccagt aacataccag    780

agaaaactga ccaagccctc atgggtgtat gggaacaaca aacctcctgg ctacttcaag    840

ggcacataac accagcaagg agcctgtcat aaccaccatc tcaaacaata gaacttccta    900

agtgaagcaa tgacttcaaa tctacttgaa ggcatggagt ataagccatg ttcctttcag    960

aggggactgt acttctgtag attactttcc ctcattaacc                         1000


<210>  14
<211>  1000
<212>  DNA
<213>  Artificial

<220>
<223>  Modified example sequence flanking at least one targeting 
       endonuclease binding site

<400>  14
tcagaccacc tcaccaccac aaagccttgc cctgaccacc ttgtaggctg gcctagacaa     60

tggtcatctt tggtgtcctc ctacccatta gagagagcca ctttcatacc agcatccagt    120

acctgaggaa taatggcctc aatggcatcc acaagtctca ctgggttggc ctgaggagca    180

gcccatgcct gtgccctagt ttaaagagga gtgatggtgc ttcctgcctc ttgagtggaa    240

ccccttgttc catagtgaga atgggaggtg tgggccattc tgacaaagga ttttggacaa    300

gtccagctat taacacctgg ctagggatta acacagtgat acctggttga aggtgggagg    360

gcaagtgatc taacaaatct gttttgcaaa ggacatcaga aatctgaggt gcctatagag    420

aacatcccca aggtttagca gaaagaactc tttaggctga ctcctggata atctttccaa    480

ggtggagcca ttaggcacac aactgggata cttgtcagta gggattaaag acctttataa    540

gtctcaaggg tttaaactta aaggacagag tttattccaa gagggaatag agcaaaggca    600

tatatgggag tgtgggactg atccctgacc tctataatgg gagaggacat taggacctaa    660

ggtgcagaat agtggccttc cttagcctct ggaagtaatg gtgggattcc ccttaacata    720

ccctcctaaa aactgagaag tgggggaccc tggggtggga tcaaaagatt agggactggt    780

gtgtaaggtg aacatgtgct gcaaggatca tcctggctgt ggttgcctca tcagctagag    840

aaagcccctg ccactaagcc atctgcaatg cctaatcttg gaacaaggaa gctgggactg    900

atccattatt gtcaaaacaa ctggtgaggg tgtgccctaa ggttggtgtt tactgattgt    960

ggtcttgggt ctagggactt ccagatatgg aaaaaattag                         1000


<210>  15
<211>  2099
<212>  DNA
<213>  Artificial

<220>
<223>  Example engineered landing pad

<400>  15
ggccggccgg atccttcatc tttggacaag ggaataaaga ctccccactt gctactaaga     60

acaataccta agttgcccag acatgactgt acccattcag agacctacca cccattaggg    120

ctatgacact aacactagcc cctggaggtt gaccatgcta ggcagtgggg gtctcaccta    180

tgacccactc agataggggt ttaaaccagt gggtgggatc tcagcctcat ataggtgttt    240

gtggtgagct ttctcctaga caagagaacc ctgaagaaca gcaagaacca gctaatatga    300

tatgtagaca tagtgggttg ctcaaatttt gtgtttagtc atattagaat tgacctcagt    360

gaccactcag aaagtgccca agcccatcta taggggccaa agtgctattg actggtgtgt    420

ctgtgaattg ttcctcccta cagagttggt gctgatatat cctagcattc tttggaaaac    480

ctagctaggg actgtcaagt gtaagatacc tcctgaattg gagggaacac tagctgccct    540

gtaccttctg gctagtacct tacaccctga atgggttagg gggtctatta tttgctggaa    600

atataccagt ttcagtaggg ctgctgcctt aggtcccaca aggtgtaaca tgtgctcaat    660

agttgcacta ccacatgcac gtgaacttaa tgatgttata gccacaacac caaccttggt    720

ttgcagtttg acatccctct ggaatgggtg tagtcatctt gctctggatc tgcctgaatc    780

attggggctg tatgcagcct gggcttaaag tgaagaatgg gatgtcccag aaatattttg    840

ggtgagaaga atcctggagt agatggtgac ctgactatcc ctgtcctatg ggcacaatct    900

atcatcagat attgcattca aagggctatc atgggatcaa gtcctaagtc aactgttgtt    960

tacctggcag acattcatct aggagttctc ttttatgcca ccccacagtg atccgccttt   1020

tgcagtttat ccactaggga caggattgcc accccacagt ggggcctcta tgcccgggac   1080

aagtgtaaaa tatagagtat aggggttatc atcacagaga agctattgct ggagggcctc   1140

tgttatttcc tctccatgcc actcccattt ttaacctacc aactgaaatc ccaagggaga   1200

ctccaccctg taactagagt cctcagaggt gagccatccc atattaacaa atgggcatta   1260

gggctaggat gccaagggat acctgaaatg ggaagttgtg gggctgagtc ctcctgggaa   1320

tcagagataa tatgtaaaca gtttgttgag agattgatga gagctgactt tgagaggtgg   1380

ccatgctccc tggtcctcaa tagggaaggc actacacaag aaacctgggt ttgatcaact   1440

gcactgtgtc ctactcacac attgtgtgcc tggaaaaatg ttacttagta tttggagggc   1500

ctccagaacc cccctgggtg caagactggg tgctagtgac tgggtgaatg agtcttggac   1560

acagtggcct tgtctaggtt gtgtgaggtg gctaggcatc atggcaatac ctcataattg   1620

atgagtgagg aaacaagact aagtccttga ctcctcttat tacatgacct ggtggatatt   1680

atgtttaaac tctgcaagct ggaatgagta ctgggtgcag atcccctggg attctggcta   1740

caaaggtgaa tgatagctag tctgtttatt agtagccaaa aaagtcagtg aggggtgagt   1800

gccctgggat gttgttaagt tcacattgca cacttggaga ccctctccat ccagtaacat   1860

accagagaaa actgaccaag ccctcatggg tgtatgggaa caacaaacct cctggctact   1920

tcaagggcac ataacaccag caaggagcct gtcataacca ccatctcaaa caatagaact   1980

tcctaagtga agcaatgact tcaaatctac ttgaaggcat ggagtataag ccatgttcct   2040

ttcagagggg actgtacttc tgtagattac tttccctcat taaccagatc tggccggcc    2099


<210>  16
<211>  2103
<212>  DNA
<213>  Artificial

<220>
<223>  Example engineered landing pad

<400>  16
ggccggccgg atcctgcact catgttcata tccatcacac ccactgaggc agatattgca     60

gtgagagggt tgcatgtagg gtcctgctac ctggccatgt aggttttggg ctgggaaaca    120

aggaaaataa tccaaggtcc caccacacac aagagcttct aaaggtcatg gtgtgctgga    180

tgagaaggcc attaagttta aacacctgtt agaggaacca gtaccttaca aggggatggt    240

ccacccttta atcatcagct tgaccaaccc ctttagtatt cacctctggc agacaccagc    300

tttgggattc ctatctaacc atttggccag tacctggcac tcacaccagt ggagactaca    360

gagctacatg aatgggtcag atccccactc catagtgcat agctggccac atgatacttt    420

accctagata tttgccatac cggagagtct gttccctggt ttcgaccggg agtgggccac    480

cttgtctttg gggcctaagc tgtggagtga tggttctggc accacctgtg ggtctgatag    540

cacatgcccc ctcctgctta tccagtggtc tgttctcatg gatattcaag gcttgtatag    600

atggaaaagg atgaggtggg tgaaggctgt tgggaagtgt gcctatgaag ccccaacaaa    660

gagattagac agtacagctg attacctaac tccttgggat gtctcaaaca aatgtttcaa    720

ggcccttttc ccttggagca tgaacctggc aatataaagt atttcatcta agggtcttat    780

acaaattacc cttttacacc attatggacc ctgaggcaag ctgtctggcc cactctagct    840

acctggcaaa tgtccacctg tagctcatac caatctttaa gctcagagca aagttcagaa    900

gcccttcagt gacagtggca atggctatcc agtgaacctg acccaattag tggcccattg    960

gattattgaa aactggcagt gctgagtcca tcaccccaaa tactgtccat aggcaagact   1020

cccgcccatc tctctatgcc cgggacaagt ggagtccatg ctcaacaccg tgcactaggg   1080

acaggattgt cagaccacct caccaccaca aagccttgcc ctgaccacct tgtaggctgg   1140

cctagacaat ggtcatcttt ggtgtcctcc tacccattag agagagccac tttcatacca   1200

gcatccagta cctgaggaat aatggcctca atggcatcca caagtctcac tgggttggcc   1260

tgaggagcag cccatgcctg tgccctagtt taaagaggag tgatggtgct tcctgcctct   1320

tgagtggaac cccttgttcc atagtgagaa tgggaggtgt gggccattct gacaaaggat   1380

tttggacaag tccagctatt aacacctggc tagggattaa cacagtgata cctggttgaa   1440

ggtgggaggg caagtgatct aacaaatctg ttttgcaaag gacatcagaa atctgaggtg   1500

cctatagaga acatccccaa ggtttagcag aaagaactct ttaggctgac tcctggataa   1560

tctttccaag gtggagccat taggcacaca actgggatac ttgtcagtag ggattaaaga   1620

cctttataag tctcaagggt ttaaacttaa aggacagagt ttattccaag agggaataga   1680

gcaaaggcat atatgggagt gtgggactga tccctgacct ctataatggg agaggacatt   1740

aggacctaag gtgcagaata gtggccttcc ttagcctctg gaagtaatgg tgggattccc   1800

cttaacatac cctcctaaaa actgagaagt gggggaccct ggggtgggat caaaagatta   1860

gggactggtg tgtaaggtga acatgtgctg caaggatcat cctggctgtg gttgcctcat   1920

cagctagaga aagcccctgc cactaagcca tctgcaatgc ctaatcttgg aacaaggaag   1980

ctgggactga tccattattg tcaaaacaac tggtgagggt gtgccctaag gttggtgttt   2040

actgattgtg gtcttgggtc tagggacttc cagatatgga aaaaattaga gatctggccg   2100

gcc                                                                 2103


<210>  17
<211>  990
<212>  DNA
<213>  Artificial

<220>
<223>  Modified example sequence flanking at least one targeting 
       endonuclease binding site

<400>  17
tgtaccaatg ttgtctccct taactaggga atatctggcc agccctcatg tataggtgtt     60

ttagtacact ctggtgagtg cattgctcag tcaactggtg gaacctgtcc ccccatccta    120

agggcaaccc cttgtaccca atttgaagat agggttgcta gagcagtgca atgaggtccc    180

ttgagtaagc agatggcctg ctaggcttca aacctcagac ctggtttttt gtatggcctg    240

ggatcagttg attatctacc ccatcatcaa taacttgccc ttaatactta aacatagggc    300

caatcattgt ggggcctcta gggaaactgt ttggtcctgg cactgtttct aaaaggggaa    360

cacatcataa tcagtaggaa acagaaaagc aagagtggag cctaccctag ttgcccagtg    420

tgggagcaaa attgatgtaa ggctacatag aacccctata ccctagtagg gtttaaacct    480

gatcattagg ctggctcccc ctgtcccata gagcacactc aatctcatgg ccaagacttg    540

aaggttaaaa tctcatgggg ctgctgatca gattgcccac aaaaggtgtt acctgataca    600

gtctaacttc ttacctgcca gcaaaaattt tgatggaagt atcctggcaa ctgtaatcct    660

ctaccactta aagattctag tattgattaa cttctctcca tacctaaaca gcagaatgta    720

gcataggttc ataggcttcc tttggaggtt cactatggaa cttaccactg gatgacaacc    780

ctcacctgtg ctactctccc taccttggag gaccattgtt gacctagaga cttatctgag    840

tgctaaccca tgacattaac ctctgggggc ttccccatcc ctggacatgt ggctggagaa    900

aatataggtc aaaaacattg agcacattag gtcgactgcc aatggtgagg ctctctaagg    960

gtctgggtta caccagatta gcagttcttg                                     990


<210>  18
<211>  1022
<212>  DNA
<213>  Artificial

<220>
<223>  Modified example sequence flanking at least one targeting 
       endonuclease binding site

<400>  18
catgggagag tgtgattcgt ctcacgcttg ctgaatctcg agtcagacta ttaggcccct     60

catcttgaac atctttgcca tccttttagc ttggttgtca gtggaaacta gggtccaatg    120

gtttagagtc cagccagatg gaggttgggc acaatgcccc tgcactatcc accctagctg    180

tagaagttat gcttgtccaa aggttatgca actgctgtct gttgggcttg agccaagaga    240

ttggtgggaa atcttgccag agttggccag ttgtctcctg tccaccaaca tgatcctata    300

tatgggtccc caccctggtt cccaagccac tcttgtaggc tgaagaggaa acacctcaga    360

agctgagtag tccaagactg cttgcaccaa gtttccaccc cttacctgtc ctgaaattaa    420

aggcaccagt gttccggttc ccttggactt tctacttgta gtgtagcact gggtggacct    480

actcccgttt aaactcctgg gatcatggca ctttctatta cagtgggtca caacttgagg    540

cagccctgta gttgagtata tagtggagag caccagatga tgattttact tggtgtaggt    600

tgtccctggc ccttagctgt ggtgacccca ttgtcatcct ccagttccaa ctttccattt    660

cctgcaatga gtggtccaca ttgctggctg ggggtggatc tatccctcca tttgggaaga    720

aacagtgata tggacactaa ccaccataag tggatggatt cttcatagag tggttgcttt    780

tgcccaagtc tgtgcagcag aggtgtcaac ctggtcccca attagtgaaa taatctcaca    840

gcctgggggc tactcgtatt aggaccagta cccattccag cctggcccaa aactttagtt    900

gagcctagac cttcccactt acatggactg attgtggcgg ttccagatga ctgttgactt    960

cctatccatt tggacttttg gaggggccag aaacctattc ctccagtata gtcctccaca   1020

tt                                                                  1022


<210>  19
<211>  2082
<212>  DNA
<213>  Artificial

<220>
<223>  Example engineered landing pad

<400>  19
tgtaccaatg ttgtctccct taactaggga atatctggcc agccctcatg tataggtgtt     60

ttagtacact ctggtgagtg cattgctcag tcaactggtg gaacctgtcc ccccatccta    120

agggcaaccc cttgtaccca atttgaagat agggttgcta gagcagtgca atgaggtccc    180

ttgagtaagc agatggcctg ctaggcttca aacctcagac ctggtttttt gtatggcctg    240

ggatcagttg attatctacc ccatcatcaa taacttgccc ttaatactta aacatagggc    300

caatcattgt ggggcctcta gggaaactgt ttggtcctgg cactgtttct aaaaggggaa    360

cacatcataa tcagtaggaa acagaaaagc aagagtggag cctaccctag ttgcccagtg    420

tgggagcaaa attgatgtaa ggctacatag aacccctata ccctagtagg gtttaaacct    480

gatcattagg ctggctcccc ctgtcccata gagcacactc aatctcatgg ccaagacttg    540

aaggttaaaa tctcatgggg ctgctgatca gattgcccac aaaaggtgtt acctgataca    600

gtctaacttc ttacctgcca gcaaaaattt tgatggaagt atcctggcaa ctgtaatcct    660

ctaccactta aagattctag tattgattaa cttctctcca tacctaaaca gcagaatgta    720

gcataggttc ataggcttcc tttggaggtt cactatggaa cttaccactg gatgacaacc    780

ctcacctgtg ctactctccc taccttggag gaccattgtt gacctagaga cttatctgag    840

tgctaaccca tgacattaac ctctgggggc ttccccatcc ctggacatgt ggctggagaa    900

aatataggtc aaaaacattg agcacattag gtcgactgcc aatggtgagg ctctctaagg    960

gtctgggtta caccagatta gcagttcttg gccttttgca gtttatctct atgcccggga   1020

caagtgaaga ctcccgccca tccaggatga ggatgaccaa catgggagag tgtgattcgt   1080

ctcacgcttg ctgaatctcg agtcagacta ttaggcccct catcttgaac atctttgcca   1140

tccttttagc ttggttgtca gtggaaacta gggtccaatg gtttagagtc cagccagatg   1200

gaggttgggc acaatgcccc tgcactatcc accctagctg tagaagttat gcttgtccaa   1260

aggttatgca actgctgtct gttgggcttg agccaagaga ttggtgggaa atcttgccag   1320

agttggccag ttgtctcctg tccaccaaca tgatcctata tatgggtccc caccctggtt   1380

cccaagccac tcttgtaggc tgaagaggaa acacctcaga agctgagtag tccaagactg   1440

cttgcaccaa gtttccaccc cttacctgtc ctgaaattaa aggcaccagt gttccggttc   1500

ccttggactt tctacttgta gtgtagcact gggtggacct actcccgttt aaactcctgg   1560

gatcatggca ctttctatta cagtgggtca caacttgagg cagccctgta gttgagtata   1620

tagtggagag caccagatga tgattttact tggtgtaggt tgtccctggc ccttagctgt   1680

ggtgacccca ttgtcatcct ccagttccaa ctttccattt cctgcaatga gtggtccaca   1740

ttgctggctg ggggtggatc tatccctcca tttgggaaga aacagtgata tggacactaa   1800

ccaccataag tggatggatt cttcatagag tggttgcttt tgcccaagtc tgtgcagcag   1860

aggtgtcaac ctggtcccca attagtgaaa taatctcaca gcctgggggc tactcgtatt   1920

aggaccagta cccattccag cctggcccaa aactttagtt gagcctagac cttcccactt   1980

acatggactg attgtggcgg ttccagatga ctgttgactt cctatccatt tggacttttg   2040

gaggggccag aaacctattc ctccagtata gtcctccaca tt                      2082


<210>  20
<211>  530
<212>  DNA
<213>  Artificial

<220>
<223>  ZFN module 2:4-MCS-2:4

<400>  20
actagtctcg agtgtccgat actggatcgt tctttcatgt agcagagcgg ttggcccaaa     60

tgtttattgg gatatcgtga gtcagatgtt cacttggtca atgggtacag tccgccgcca    120

gtgtctattt gagcctctct gcaagactcc cgcccatctc tctatgcccg ggacaagtgt    180

ggacgacaag cgagtgcagc cggcttacag ccttttgcag tttatctcta tgcccgggac    240

aagtgattaa taagctttta attaacctgc agggcatgcg tcgacaagac tcccgcccat    300

ctctctatgc ccgggacaag tgtcgagagg aatggccatg agtatttggt ttgccttttg    360

cagtttatct ctatgcccgg gacaagtgca gagcggttgg cccaaatgtt tattgggata    420

tcgtgagtca gatgttcact tggtcaatgg gtacagtccg ccgccagtgt ctatttgagc    480

ctctctgcgt acatgataac ctcagttggc gagcgttgct cgagactagt               530


<210>  21
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide 610F

<400>  21
gctactaaga acaataccta agttgc                                          26


<210>  22
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide 611F

<400>  22
tgcactcatg ttcatatcc                                                  19


<210>  23
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide 611R

<400>  23
tgtacaagaa agctgggtg                                                  19


<210>  24
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide YFPF_3302

<400>  24
tatggtccag agttgaagg                                                  19


<210>  25
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide YFPR_4577

<400>  25
tcatctgcac aactggtga                                                  19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide YFPF_4302

<400>  26
tctttcccaa cacatgacc                                                  19


<210>  27
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer TQPATS

<400>  27
acaagagtgg attgatgatc tagagaggt                                       29


<210>  28
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer TQPATA

<400>  28
ctttgatgcc tatgtgacac gtaaacagt                                       29


<210>  29
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer TQPATFQ

<400>  29
ggtgttgtgg ctggtattgc ttacgctgg                                       29


<210>  30
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer ZGP3S

<400>  30
cctgctccac taccagtaca a                                               21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer ZGP3A

<400>  31
gtccaagaag gtgaccttct c                                               21


<210>  32
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Primer TQZGP3

<400>  32
agatcaccga ctttgcgctc ttt                                             23


<210>  33
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer GAAD1F

<400>  33
tgttcggttc cctctaccaa                                                 20


<210>  34
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer GAAD1R

<400>  34
caacatccat caccttgact ga                                              22


<210>  35
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Primer GAAD1P

<400>  35
cacagaaccg tcgcttcagc aaca                                            24


<210>  36
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Primer IVF-Taq

<400>  36
tggcggacga cgacttgt                                                   18


<210>  37
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Primer INR-Taq

<400>  37
aaagtttgga ggctgccgt                                                  19


<210>  38
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer IV-Probe

<400>  38
cgagcagacc gccgtgtact tctacc                                          26


