                         SEQUENCE LISTING

<110>  The Chinese University of Hong Kong
           SUNG, Jao Yiu Joseph
           YU, Jun
           CHEUNG, Kin Fai
 
<120>  MARKER FOR COLON CANCER AND METHOD FOR DETECTING COLON CANCER

<130>  11C50178

<150>  US12/764,337
<151>  2010-04-21

<160>  29    

<170>  PatentIn version 3.3

<210>  1
<211>  298
<212>  DNA
<213>  Homo sapiens

<400>  1
cactgcagcc tggctgagag aaaggacgcg ggttgtgctc tctggaagca aaggggtctg     60

cggcccagct ggcctgggag cttgtggccg gcgctggaag ctgcccgctc tccccgcggg    120

cctgaccttg gctcccgccg cagctctgcc ggccgactgc ctccctgcac attttgctgc    180

cgttccagtc cttacaggac cgggcctgga gccaggccat gcttcggaaa gccctggggg    240

ttggggacgc gcaaaaccca gaatcgaacc ccgaagctgg gggcaagtcc agattcag      298


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward transcript primer

<400>  2
ctcaagctca ccaacaacca                                                 20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse transcript primer

<400>  3
caggaaagac gtgagtgcag                                                 20


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter forward primer

<400>  4
tattgtagtt tggttgagag aaagga                                          26


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter reverse primer

<400>  5
ctaaatctaa acttaccccc aacttc                                          26


<210>  6
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BstUI cleavage sequence

<400>  6
cgcg                                                                   4


<210>  7
<211>  151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  151bp amplified portion of human TBX5 transcript variant No. 1

<400>  7
ctcaagctca ccaacaacca cctggaccca tttgggcata ttattctaaa ttccatgcac     60

aaataccagc ctagattaca catcgtgaaa gcggatgaaa ataatggatt tggctcaaaa    120

aatacagcgt tctgcactca cgtctttcct g                                   151


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  8
gggttgtgtt ttttggaagt aaa                                             23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  9
gtttagttgg tttgggagtt tgt                                             23


<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  10
gattgttttt ttgtatattt tgttgt                                          26


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Antisense primer

<400>  11
acaacaaaat atacaaaaaa acaatc                                          26


<210>  12
<211>  518
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Ala Asp Ala Asp Glu Gly Phe Gly Leu Ala His Thr Pro Leu Glu 
1               5                   10                  15      


Pro Asp Ala Lys Asp Leu Pro Cys Asp Ser Lys Pro Glu Ser Ala Leu 
            20                  25                  30          


Gly Ala Pro Ser Lys Ser Pro Ser Ser Pro Gln Ala Ala Phe Thr Gln 
        35                  40                  45              


Gln Gly Met Glu Gly Ile Lys Val Phe Leu His Glu Arg Glu Leu Trp 
    50                  55                  60                  


Leu Lys Phe His Glu Val Gly Thr Glu Met Ile Ile Thr Lys Ala Gly 
65                  70                  75                  80  


Arg Arg Met Phe Pro Ser Tyr Lys Val Lys Val Thr Gly Leu Asn Pro 
                85                  90                  95      


Lys Thr Lys Tyr Ile Leu Leu Met Asp Ile Val Pro Ala Asp Asp His 
            100                 105                 110         


Arg Tyr Lys Phe Ala Asp Asn Lys Trp Ser Val Thr Gly Lys Ala Glu 
        115                 120                 125             


Pro Ala Met Pro Gly Arg Leu Tyr Val His Pro Asp Ser Pro Ala Thr 
    130                 135                 140                 


Gly Ala His Trp Met Arg Gln Leu Val Ser Phe Gln Lys Leu Lys Leu 
145                 150                 155                 160 


Thr Asn Asn His Leu Asp Pro Phe Gly His Ile Ile Leu Asn Ser Met 
                165                 170                 175     


His Lys Tyr Gln Pro Arg Leu His Ile Val Lys Ala Asp Glu Asn Asn 
            180                 185                 190         


Gly Phe Gly Ser Lys Asn Thr Ala Phe Cys Thr His Val Phe Pro Glu 
        195                 200                 205             


Thr Ala Phe Ile Ala Val Thr Ser Tyr Gln Asn His Lys Ile Thr Gln 
    210                 215                 220                 


Leu Lys Ile Glu Asn Asn Pro Phe Ala Lys Gly Phe Arg Gly Ser Asp 
225                 230                 235                 240 


Asp Met Glu Leu His Arg Met Ser Arg Met Gln Ser Lys Glu Tyr Pro 
                245                 250                 255     


Val Val Pro Arg Ser Thr Val Arg Gln Lys Val Ala Ser Asn His Ser 
            260                 265                 270         


Pro Phe Ser Ser Glu Ser Arg Ala Leu Ser Thr Ser Ser Asn Leu Gly 
        275                 280                 285             


Ser Gln Tyr Gln Cys Glu Asn Gly Val Ser Gly Pro Ser Gln Asp Leu 
    290                 295                 300                 


Leu Pro Pro Pro Asn Pro Tyr Pro Leu Pro Gln Glu His Ser Gln Ile 
305                 310                 315                 320 


Tyr His Cys Thr Lys Arg Lys Glu Glu Glu Cys Ser Thr Thr Asp His 
                325                 330                 335     


Pro Tyr Lys Lys Pro Tyr Met Glu Thr Ser Pro Ser Glu Glu Asp Ser 
            340                 345                 350         


Phe Tyr Arg Ser Ser Tyr Pro Gln Gln Gln Gly Leu Gly Ala Ser Tyr 
        355                 360                 365             


Arg Thr Glu Ser Ala Gln Arg Gln Ala Cys Met Tyr Ala Ser Ser Ala 
    370                 375                 380                 


Pro Pro Ser Glu Pro Val Pro Ser Leu Glu Asp Ile Ser Cys Asn Thr 
385                 390                 395                 400 


Trp Pro Ser Met Pro Ser Tyr Ser Ser Cys Thr Val Thr Thr Val Gln 
                405                 410                 415     


Pro Met Asp Arg Leu Pro Tyr Gln His Phe Ser Ala His Phe Thr Ser 
            420                 425                 430         


Gly Pro Leu Val Pro Arg Leu Ala Gly Met Ala Asn His Gly Ser Pro 
        435                 440                 445             


Gln Leu Gly Glu Gly Met Phe Gln His Gln Thr Ser Val Ala His Gln 
    450                 455                 460                 


Pro Val Val Arg Gln Cys Gly Pro Gln Thr Gly Leu Gln Ser Pro Gly 
465                 470                 475                 480 


Thr Leu Gln Pro Pro Glu Phe Leu Tyr Ser His Gly Val Pro Arg Thr 
                485                 490                 495     


Leu Ser Pro His Gln Tyr His Ser Val His Gly Val Gly Met Val Pro 
            500                 505                 510         


Glu Trp Ser Asp Asn Ser 
        515             




<210>  15
<211>  3921
<212>  DNA
<213>  Homo sapiens

<400>  15
catgccttat gcaagagacc tcagtccccc ggaacaactc gatttccttc caatagaggt     60

ctgaggtgga ctcccacctc ccttcgtgaa gagttccctc ctctccccct tcctaagaaa    120

gtcgatcttg gctctatttg tgtcttatgt tcatcaccct cattcctccg gagaaagccg    180

ggttggttta tgtctttatt tattcccggg gccaagacgt ccggaacctg tggctgcgca    240

gacccggcac tgataggcga agacggagag aaatttacct cccgccgctg ccccccagcc    300

aaacgtgaca gcgcgcgggc cggttgcgtg actcgtgacg tctccaagtc ctataggtgc    360

agcggctggt gagatagtcg ctatcgcctg gttgcctctt tattttactg gggtatgcct    420

ggtaataaac agtaatattt aatttgtcgg agaccacaaa ccaaccttga gctgggaggt    480

acgtgctctt cttgacagac gttggaagaa gacctggcct aaagaggtct cttttggtgg    540

tccttttcaa agtcttcacc tgagccctgc tctccagcga ggcgcactcc tggcttttgc    600

gctccaaaga agaggtggga tagttggaga gcagaacctt gcgcgggcac agggccctgg    660

gcgcaccatg gccgacgcag acgagggctt tggcctggcg cacacgcctc tggagcctga    720

cgcaaaagac ctgccctgcg attcgaaacc cgagagcgcg ctcggggccc ccagcaagtc    780

cccgtcgtcc ccgcaggccg ccttcaccca gcagggcatg gagggaatca aagtgtttct    840

ccatgaaaga gaactgtggc taaaattcca cgaagtgggc acggaaatga tcataaccaa    900

ggctggaagg cggatgtttc ccagttacaa agtgaaggtg acgggcctta atcccaaaac    960

gaagtacatt cttctcatgg acattgtacc tgccgacgat cacagataca aattcgcaga   1020

taataaatgg tctgtgacgg gcaaagctga gcccgccatg cctggccgcc tgtacgtgca   1080

cccagactcc cccgccaccg gggcgcattg gatgaggcag ctcgtctcct tccagaaact   1140

caagctcacc aacaaccacc tggacccatt tgggcatatt attctaaatt ccatgcacaa   1200

ataccagcct agattacaca tcgtgaaagc ggatgaaaat aatggatttg gctcaaaaaa   1260

tacagcgttc tgcactcacg tctttcctga gactgcgttt atagcagtga cttcctacca   1320

gaaccacaag atcacgcaat taaagattga gaataatccc tttgccaaag gatttcgggg   1380

cagtgatgac atggagctgc acagaatgtc aagaatgcaa agtaaagaat atcccgtggt   1440

ccccaggagc accgtgaggc aaaaagtggc ctccaaccac agtcctttca gcagcgagtc   1500

tcgagctctc tccacctcat ccaatttggg gtcccaatac cagtgtgaga atggtgtttc   1560

cggcccctcc caggacctcc tgcctccacc caacccatac ccactgcccc aggagcatag   1620

ccaaatttac cattgtacca agaggaaaga ggaagaatgt tccaccacag accatcccta   1680

taagaagccc tacatggaga catcacccag tgaagaagat tccttctacc gctctagcta   1740

tccacagcag cagggcctgg gtgcctccta caggacagag tcggcacagc ggcaagcttg   1800

catgtatgcc agctctgcgc cccccagcga gcctgtgccc agcctagagg acatcagctg   1860

caacacgtgg ccaagcatgc cttcctacag cagctgcacc gtcaccaccg tgcagcccat   1920

ggacaggcta ccctaccagc acttctccgc tcacttcacc tcggggcccc tggtccctcg   1980

gctggctggc atggccaacc atggctcccc acagctggga gagggaatgt tccagcacca   2040

gacctccgtg gcccaccagc ctgtggtcag gcagtgtggg cctcagactg gcctgcagtc   2100

ccctggcacc cttcagcccc ctgagttcct ctactctcat ggcgtgccaa ggactctatc   2160

ccctcatcag taccactctg tgcacggagt tggcatggtg ccagagtgga gcgacaatag   2220

ctaaagtgag gcctgcttca caacagacat ttcctagaga aagagagaga gagaggagaa   2280

agagagagaa ggagagagac agtagccaag agaaccccac ggacaagatt tttcatttca   2340

cccaatgttc acatctgcac tcaaggtcgc tggatgctga tctaatcagt agcttgaaac   2400

cacaatttta aaaatgtgac tttcttgttt tgtctcaaaa cttaaaaaaa caaacacaaa   2460

aagatgagtc ccacccccca ctaccaccac acccatcaac cagccacatt cacgctactc   2520

cccagatctc ttcccccatt ccttcttttg ggctctagaa agtcttgcct cattgagtgt   2580

ttttccctag tgcgtagttg gagtctgtcc ctgtcttggt gttaatgttg acattgttat   2640

ataataaatg ataatatatt tttttctttc aattttctta atgggaccca gtcccttatt   2700

tggggggagg tctgaggcaa gtatatttca aaatatgtac ttgcgggatt cccttcaagt   2760

aaaccatccc tgaaacctaa attcacgttt ccccttgact aagaaaagca cctacctctg   2820

ccatgtgatg tttctgaaaa gcctctgtat gtccccattt gctttggttt tgtcctgcct   2880

tctccaatat cacgtgctca gttttgcctc tacttaccca tggagtcagg ataacactga   2940

cgctccctgg catcctatct tattcagccc taccatcttg ccagctctgt ctttccagct   3000

gtctgtcgct aaaacgtggc ctatagcttc ccttccggaa agcttgcttt gaaaaactta   3060

aaaagccccc gtttacatgt aggcaggact gtgataacag tgcaagctct gtgttgacaa   3120

gagttgtgga caaaaagcca aaataaatat tcttcctgat taaaaaaatt ttttttgaaa   3180

aaaacaaggc cagccccaac cttccaaacc tccatcacca acaacccaaa ctggatgtca   3240

agcaaaatgc acaattccta cagaagaggc aagacacagt caccaatgat atctcgccaa   3300

agaaaccacg cccacaccaa tgccaacaca aaactgtgtt tactgaaagc cgaaaacagt   3360

attaaaaaaa gtgtgtaagt aaagtgttat ggtagggttc ttcagatgta atattttact   3420

ggtactattt atttataaat aggaattcta attaagtaat aacatgaaat gaaacccagc   3480

ataggagctg gccaagagct tttaatttta ttgatactca aaaccaagtt tgtgtttttt   3540

tgtttttttt tgtttttttc ctctttcgaa tgtgctttgc tttttttgat taaaaagaat   3600

tttttttttc cttttttata aacagaccct aataaagaga acagggtaag atgtgaggct   3660

gagtgtgttt aagtacgtga gagagtgtga gtgtgtttgt aagtgagtgt ccctatgcga   3720

ttatgtctct ttacgttgct aaggggggag ggtgaggatt aagtactcgt gccttatatt   3780

tgtgtgccaa ttaatgccta ataaatacca tgtgcttaaa caagtaaaaa aaaaaaaaaa   3840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa   3900

aaaaaaaaaa aaaaaaaaaa a                                             3921


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  16
taggcgaaga cggagagaaa                                                 20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  17
gctcaaggtt ggtttgtggt                                                 20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  18
accacaaacc aaccttgagc                                                 20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  19
gcaaggttct gctctccaac                                                 20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  20
tcataaccaa ggctggaagg                                                 20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  21
gcccgtcaca gaccatttat                                                 20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  22
agctctctcc acctcatcca                                                 20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  23
ttcactgggt gatgtctcca                                                 20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  24
catcaaccag ccacattcac                                                 20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  25
agggactggg tcccattaag                                                 20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  26
tttggttttg tcctgccttc                                                 20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  27
ccacgtttta gcgacagaca                                                 20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense primer

<400>  28
ccaatgccaa cacaaaactg                                                 20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-sense primer

<400>  29
agctcctatg ctgggtttca                                                 20


