                         SEQUENCE LISTING

<110>  Texas A&M University Systems
       Mirkov, T. Erik
       Park, Jong Won
       Gao, San-Ji
 
<120>  Compositions, Organisms, Systems, and Methods for Expressing a 
       Gene Product in Plants

<130>  16603.105035

<140>  Not Yet Assigned
<141>  Filed Herewith

<150>  US 61/395,197
<151>  2010-05-10

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  1816
<212>  DNA
<213>  Sugarcane bacilliform virus

<220>
<221>  promoter
<222>  (1)..(1786)
<223>  SCBV21 Promoter

<220>
<221>  misc_signal
<222>  (520)..(524)
<223>  Possible W-box WBOXATNPR1

<220>
<221>  misc_signal
<222>  (1055)..(1104)
<223>  Transcription start site TSSI

<220>
<221>  misc_signal
<222>  (1162)..(1166)
<223>  Possible W-box WBOXATNPR1

<220>
<221>  misc_signal
<222>  (1309)..(1313)
<223>  Possible W-box WBOXATNPR1

<220>
<221>  CAAT_signal
<222>  (1475)..(1478)
<223>  Possible CAAT signal

<220>
<221>  misc_signal
<222>  (1502)..(1509)
<223>  Possible auxin responsive element AUXRETGA1GMGH3

<220>
<221>  CAAT_signal
<222>  (1511)..(1514)
<223>  Possible CAAT signal

<220>
<221>  CAAT_signal
<222>  (1540)..(1543)
<223>  Possible CAAT signal

<220>
<221>  CAAT_signal
<222>  (1558)..(1561)
<223>  Possible CAAT signal

<220>
<221>  misc_signal
<222>  (1737)..(1786)
<223>  Transcription start site TSS2

<220>
<221>  TATA_signal
<222>  (1747)..(1753)
<223>  Possible TATA signal

<220>
<221>  misc_signal
<222>  (1787)..(1787)
<223>  Transcription start site

<400>  1
gaagaacagc atgctgaaca tctgtggaag atgctacaga tatgcaagaa gaatgggtta       60

atcttaagcc cttccaagta taaattggag taaaaagagt tgactttctt ggttcaacaa      120

ttggagataa tcagttagct gttcaagaac atatagtctc caagatagct gattttgatg      180

aagaacgtct caagaccaag gaaggactga aaagctggct ggcaacactc aattatgcca      240

gaaatcacat caaggatatg ggaaaactcc ttggaccctt atatcctaaa acttcagaaa      300

agggagcaaa aggattaaat tctgaagatt ggaaattaat cagcagaatc aagacaatgg      360

tcagaaatct gccaaatctg actattccac cagaggatgc atatattatc attgaaacag      420

atgcttgtgc aactggttgg ggtgcagttt gcaaatggaa gaaatccaag gcagacccaa      480

gaagctccga gctcatatgt cgatatgcaa gtgggaaatt tgacaaacca aaagggacat      540

gtgatgcaga aatctatgga gtaatgaatg ggctggagaa aatgagactc ttttatcttg      600

ataaaaggga aatcactgtg aggacagata gtgccgcaat agagaggttc tacaacaaga      660

gtgttgaaca taaaccctca gaaatccgtt ggataaggtt tatggactat atcactggag      720

caggaccaaa gattgtgatt gagcatatca aaggaaaaca caatggtctg gcagatatcc      780

tctcaagatt gaaagcaaaa ctggcagaat caccttcaga agaagtggtt ttacttgcga      840

aagctttaaa ggaagttgca tactatcctg aacacccgca agtgccaaaa ctaattgaat      900

ggggaaagca aattcttgat ccatttccca agttcaagaa ggacatgttt gaaaaaactg      960

aacacatcat gatggctagt caagagccta cactgctttg tggatgtaga aggcctgcat     1020

atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa tgtgcaatga     1080

acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa gaacgaattg     1140

aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta ccaagtacat     1200

caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag gataaagaag     1260

ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt gacagaccaa     1320

ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg cagaagatca     1380

aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt gagttctggt     1440

tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca gttgatctta     1500

ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac ggaaggacaa     1560

ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt tcggcgccag     1620

caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct tacctttgtc     1680

ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt gtgtgttccc     1740

tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc accacatgat     1800

catttgagca agtttg                                                     1816

<210>  2
<211>  4035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SCBV21-GUS-NOS

<220>
<221>  promoter
<222>  (30)..(1845)
<223>  SCBV21 Promoter

<220>
<221>  misc_feature
<222>  (1893)..(3704)
<223>  GUS coding sequence

<220>
<221>  terminator
<222>  (3777)..(4030)
<223>  NOS

<400>  2
aagcttgggc cgcgaattca ctagtgattg aagaacagca tgctgaacat ctgtggaaga       60

tgctacagat atgcaagaag aatgggttaa tcttaagccc ttccaagtat aaattggagt      120

aaaaagagtt gactttcttg gttcaacaat tggagataat cagttagctg ttcaagaaca      180

tatagtctcc aagatagctg attttgatga agaacgtctc aagaccaagg aaggactgaa      240

aagctggctg gcaacactca attatgccag aaatcacatc aaggatatgg gaaaactcct      300

tggaccctta tatcctaaaa cttcagaaaa gggagcaaaa ggattaaatt ctgaagattg      360

gaaattaatc agcagaatca agacaatggt cagaaatctg ccaaatctga ctattccacc      420

agaggatgca tatattatca ttgaaacaga tgcttgtgca actggttggg gtgcagtttg      480

caaatggaag aaatccaagg cagacccaag aagctccgag ctcatatgtc gatatgcaag      540

tgggaaattt gacaaaccaa aagggacatg tgatgcagaa atctatggag taatgaatgg      600

gctggagaaa atgagactct tttatcttga taaaagggaa atcactgtga ggacagatag      660

tgccgcaata gagaggttct acaacaagag tgttgaacat aaaccctcag aaatccgttg      720

gataaggttt atggactata tcactggagc aggaccaaag attgtgattg agcatatcaa      780

aggaaaacac aatggtctgg cagatatcct ctcaagattg aaagcaaaac tggcagaatc      840

accttcagaa gaagtggttt tacttgcgaa agctttaaag gaagttgcat actatcctga      900

acacccgcaa gtgccaaaac taattgaatg gggaaagcaa attcttgatc catttcccaa      960

gttcaagaag gacatgtttg aaaaaactga acacatcatg atggctagtc aagagcctac     1020

actgctttgt ggatgtagaa ggcctgcata tcagttcaca tctggcacaa aactcaaccc     1080

aagcaggaag ttctataaat gtgcaatgaa catgtgccac tgctggtatt gggcagatct     1140

tttagaagaa tatgtccaag aacgaattga agtgttcatg attgagaact ttgacaagaa     1200

aatgggaatt caagatgtac caagtacatc aaatgctaac attccaggaa attttaaatc     1260

tcttgcagat ttgaagaagg ataaagaagc taaagctgaa tatcaagaca tgcttgataa     1320

tcatcgttca agcattattg acagaccaag gccaacagat gaacacttca agcctggata     1380

catgtacacc gattccctgc agaagatcaa ggaggactac gcaagcccaa gacaggagga     1440

accaccatga gaagacattg agttctggtt atgcaaggaa gaagactacc acacagaaga     1500

cctcaataca gaagatgcag ttgatcttac tgacgtaagc aatgacgatc agtggaggcg     1560

atcgtaagca atgatgcacg gaaggacaat tatggagcgt ggaggaccca tcaagcactc     1620

agaacgcgaa cctcaacttt cggcgccagc accttgtatc tttagttggt gtgtgtcttt     1680

ttcggcatct gtgccacctt acctttgtcg gccacgttgc ctatgcttag cacctacgca     1740

agcatagcgc tcggctggtg tgtgttccct ctgcctatat aaggcatggt tgtaagactc     1800

ttacactcat cggtagttca ccacatgatc atttgagcaa gtttgaatcg aattcccgcg     1860

gccctagagg atccccgggt ggtcagtccc ttatgttacg tcctgtagaa accccaaccc     1920

gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa     1980

ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct gtgccaggca     2040

gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac gtctggtatc     2100

agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg     2160

cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg     2220

gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta     2280

tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta     2340

ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa     2400

tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg     2460

tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg     2520

gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca     2580

ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct     2640

atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg     2700

gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact     2760

ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc     2820

tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc     2880

attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg     2940

atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca     3000

agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac     3060

aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta     3120

ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg     3180

aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg     3240

acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg     3300

gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc     3360

tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt     3420

tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc     3480

tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga     3540

atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga     3600

tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg     3660

gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac aactctcctg     3720

gcgcaccatc gtcggctaca gcctcgggaa ttgctaccga gctcgaattt ccccgatcgt     3780

tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt     3840

atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg     3900

ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata     3960

gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta     4020

ctagatcggg aattc                                                      4035

<210>  3
<211>  2877
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SCBV21-EYFP-NOS

<220>
<221>  promoter
<222>  (37)..(1852)
<223>  SCBV21 Promoter

<220>
<221>  misc_feature
<222>  (1874)..(2602)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2612)..(2865)
<223>  NOS

<400>  3
gtcgacctgc aggcggccgc gaattcacta gtgattgaag aacagcatgc tgaacatctg       60

tggaagatgc tacagatatg caagaagaat gggttaatct taagcccttc caagtataaa      120

ttggagtaaa aagagttgac tttcttggtt caacaattgg agataatcag ttagctgttc      180

aagaacatat agtctccaag atagctgatt ttgatgaaga acgtctcaag accaaggaag      240

gactgaaaag ctggctggca acactcaatt atgccagaaa tcacatcaag gatatgggaa      300

aactccttgg acccttatat cctaaaactt cagaaaaggg agcaaaagga ttaaattctg      360

aagattggaa attaatcagc agaatcaaga caatggtcag aaatctgcca aatctgacta      420

ttccaccaga ggatgcatat attatcattg aaacagatgc ttgtgcaact ggttggggtg      480

cagtttgcaa atggaagaaa tccaaggcag acccaagaag ctccgagctc atatgtcgat      540

atgcaagtgg gaaatttgac aaaccaaaag ggacatgtga tgcagaaatc tatggagtaa      600

tgaatgggct ggagaaaatg agactctttt atcttgataa aagggaaatc actgtgagga      660

cagatagtgc cgcaatagag aggttctaca acaagagtgt tgaacataaa ccctcagaaa      720

tccgttggat aaggtttatg gactatatca ctggagcagg accaaagatt gtgattgagc      780

atatcaaagg aaaacacaat ggtctggcag atatcctctc aagattgaaa gcaaaactgg      840

cagaatcacc ttcagaagaa gtggttttac ttgcgaaagc tttaaaggaa gttgcatact      900

atcctgaaca cccgcaagtg ccaaaactaa ttgaatgggg aaagcaaatt cttgatccat      960

ttcccaagtt caagaaggac atgtttgaaa aaactgaaca catcatgatg gctagtcaag     1020

agcctacact gctttgtgga tgtagaaggc ctgcatatca gttcacatct ggcacaaaac     1080

tcaacccaag caggaagttc tataaatgtg caatgaacat gtgccactgc tggtattggg     1140

cagatctttt agaagaatat gtccaagaac gaattgaagt gttcatgatt gagaactttg     1200

acaagaaaat gggaattcaa gatgtaccaa gtacatcaaa tgctaacatt ccaggaaatt     1260

ttaaatctct tgcagatttg aagaaggata aagaagctaa agctgaatat caagacatgc     1320

ttgataatca tcgttcaagc attattgaca gaccaaggcc aacagatgaa cacttcaagc     1380

ctggatacat gtacaccgat tccctgcaga agatcaagga ggactacgca agcccaagac     1440

aggaggaacc accatgagaa gacattgagt tctggttatg caaggaagaa gactaccaca     1500

cagaagacct caatacagaa gatgcagttg atcttactga cgtaagcaat gacgatcagt     1560

ggaggcgatc gtaagcaatg atgcacggaa ggacaattat ggagcgtgga ggacccatca     1620

agcactcaga acgcgaacct caactttcgg cgccagcacc ttgtatcttt agttggtgtg     1680

tgtctttttc ggcatctgtg ccaccttacc tttgtcggcc acgttgccta tgcttagcac     1740

ctacgcaagc atagcgctcg gctggtgtgt gttccctctg cctatataag gcatggttgt     1800

aagactctta cactcatcgg tagttcacca catgatcatt tgagcaagtt tgaatcgaat     1860

tcccgcggcc gccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct     1920

ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg     1980

cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt     2040

gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc     2100

cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga     2160

gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga     2220

gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa     2280

catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga     2340

caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag     2400

cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct     2460

gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg     2520

cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga     2580

gctgtacaag agatctatct agcgagctcg atcgttcaaa catttggcaa taaagtttct     2640

taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg     2700

ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga     2760

ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact     2820

aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcggggatgg gggatcc        2877

<210>  4
<211>  3038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette 35S-GUS-NOS

<220>
<221>  promoter
<222>  (25)..(859)
<223>  35S Promoter

<220>
<221>  misc_feature
<222>  (896)..(2707)
<223>  GUS coding sequence

<220>
<221>  terminator
<222>  (2780)..(3033)
<223>  NOS

<300>
<308>  AF502128
<309>  2002-06-05
<313>  (1)..(3038)

<400>  4
aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc       60

cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata      120

atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga      180

ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag      240

tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta      300

aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa      360

cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca      420

agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca      480

aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg      540

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa      600

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg      660

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag      720

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa      780

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat      840

ttcatttgga gagaacacgg gggactctag aggatccccg ggtggtcagt cccttatgtt      900

acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag      960

tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag     1020

ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa     1080

ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca     1140

gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca     1200

ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt     1260

tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac     1320

tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca     1380

tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac     1440

ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt     1500

tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca     1560

ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg     1620

gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg     1680

tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct     1740

gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg     1800

tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg     1860

ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga     1920

tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg     1980

cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg     2040

ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca     2100

cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg     2160

ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac     2220

ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt     2280

gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga     2340

gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat     2400

caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag     2460

tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc     2520

cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg     2580

cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt     2640

tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa     2700

acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac     2760

cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc     2820

ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa     2880

taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc     2940

aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat     3000

cgcgcgcggt gtcatctatg ttactagatc gggaattc                             3038

<210>  5
<211>  1893
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette 35S-EYFP-NOS

<220>
<221>  promoter
<222>  (25)..(859)
<223>  35S Promoter

<220>
<221>  misc_feature
<222>  (879)..(1607)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (1617)..(1870)
<223>  NOS

<400>  5
aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc       60

cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata      120

atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga      180

ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag      240

tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta      300

aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa      360

cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca      420

agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca      480

aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg      540

gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa      600

aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg      660

cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag      720

aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa      780

gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat      840

ttcatttgga gagaacacgg gggactctag aggatcccat ggtgagcaag ggcgaggagc      900

tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt      960

tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca     1020

tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg     1080

gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg     1140

ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca     1200

agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg     1260

gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca     1320

gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga     1380

tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc     1440

ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc     1500

tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg     1560

ccgggatcac tctcggcatg gacgagctgt acaagagatc tatctagcga gctcgatcgt     1620

tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt     1680

atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg     1740

ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata     1800

gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta     1860

ctagatcggg gaattcctgc agcccgggga tcc                                  1893

<210>  6
<211>  1890
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette E35S-EYFP-NOS

<220>
<221>  promoter
<222>  (73)..(829)
<223>  E35S Promoter

<220>
<221>  misc_feature
<222>  (887)..(1615)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (1625)..(1878)
<223>  NOS

<400>  6
aagcttgatc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc       60

tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag      120

atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa      180

acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg      240

aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct      300

ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag      360

acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca      420

ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga      480

cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc      540

acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata      600

aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac      660

ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt      720

gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc      780

cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc      840

tctctacaaa tctatctctc tcgattcgca gatctgtcga tcgaccatgg tgagcaaggg      900

cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg      960

ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct     1020

gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt     1080

cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt     1140

caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg     1200

caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga     1260

gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa     1320

ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa     1380

cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca     1440

gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca     1500

gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt     1560

gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagagatcta tctagcgagc     1620

tcgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg     1680

cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat     1740

gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat     1800

acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat     1860

ctatgttact agatcgggga tgggggatcc                                      1890

<210>  7
<211>  4178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette Pr4-GUS-NOS

<220>
<221>  promoter
<222>  (1)..(966)
<223>  Pr4 Promoter

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (671)..(671)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (967)..(1977)
<223>  First exon (5'UTR) and first intron

<220>
<221>  misc_feature
<222>  (2036)..(3847)
<223>  GUS coding sequence

<220>
<221>  terminator
<222>  (3920)..(4173)
<223>  NOS

<400>  7
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc       60

attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt      120

gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata      180

gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta      240

aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt      300

gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta      360

catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt      420

ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta      480

ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta      540

agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt      600

aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc      660

aagcgaanca nacggcacgg natctctgtc gctgcctcca ccgttggact tgctccgctg      720

tcggcatcca gaaattgcgt ggcggcaggc agacgtgagc cggcacgagg cggcctcctc      780

ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct      840

tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt      900

gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc      960

ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt     1020

ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt     1080

ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt     1140

tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc     1200

cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggntt ggtttgccct     1260

tttcctttat ttcaatatat gccgtgccac ttgtttgtcg ggtcatcttt tcatngcttt     1320

tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa     1380

ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca     1440

tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat     1500

gttgatgcgg gttttactga tgcatataca gagatgcttt tnttcgcttg gttgtgatga     1560

tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac     1620

tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac     1680

gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta     1740

ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc     1800

tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga     1860

tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt     1920

gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc     1980

gactctagag gatctgatat ctgatcagaa gacaccatgg ggtggtcagt cccttatgtt     2040

acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag     2100

tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag     2160

ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa     2220

ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca     2280

gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca     2340

ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt     2400

tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac     2460

tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca     2520

tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac     2580

ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt     2640

tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca     2700

ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg     2760

gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg     2820

tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct     2880

gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg     2940

tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg     3000

ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga     3060

tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg     3120

cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg     3180

ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca     3240

cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg     3300

ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac     3360

ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt     3420

gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga     3480

gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat     3540

caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag     3600

tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc     3660

cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg     3720

cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt     3780

tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa     3840

acaatgaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac     3900

cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc     3960

ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa     4020

taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc     4080

aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat     4140

cgcgcgcggt gtcatctatg ttactagatc gggaattc                             4178

<210>  8
<211>  3020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette Pr4-EYFP-NOS

<220>
<221>  promoter
<222>  (1)..(966)
<223>  Pr4 Promoter

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (671)..(671)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (967)..(1977)
<223>  First exon (5'UTR) and first intron

<220>
<221>  misc_feature
<222>  (2017)..(2745)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2755)..(3008)
<223>  NOS

<400>  8
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc       60

attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt      120

gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata      180

gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta      240

aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt      300

gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta      360

catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt      420

ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta      480

ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta      540

agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt      600

aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc      660

aagcgaanca nacggcacgg natctctgtc gctgcctcca ccgttggact tgctccgctg      720

tcggcatcca gaaattgcgt ggcggcaggc agacgtgagc cggcacgagg cggcctcctc      780

ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct      840

tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt      900

gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc      960

ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt     1020

ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt     1080

ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt     1140

tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc     1200

cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggntt ggtttgccct     1260

tttcctttat ttcaatatat gccgtgccac ttgtttgtcg ggtcatcttt tcatngcttt     1320

tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa     1380

ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca     1440

tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat     1500

gttgatgcgg gttttactga tgcatataca gagatgcttt tnttcgcttg gttgtgatga     1560

tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac     1620

tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac     1680

gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta     1740

ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc     1800

tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga     1860

tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt     1920

gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc     1980

gactctagag gatctgatat ctgatcagaa gacaccatgg tgagcaaggg cgaggagctg     2040

ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc     2100

agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc     2160

tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc     2220

ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc     2280

atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag     2340

acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc     2400

atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc     2460

cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc     2520

cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc     2580

atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg     2640

agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc     2700

gggatcactc tcggcatgga cgagctgtac aagagatcta tctagcgagc tcgatcgttc     2760

aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat     2820

catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt     2880

atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga     2940

aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact     3000

agatcgggga tgggggatcc                                                 3020

<210>  9
<211>  4102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette Ubi-GUS-NOS

<220>
<221>  promoter
<222>  (1)..(995)
<223>  Ubi Promoter

<220>
<221>  misc_feature
<222>  (996)..(2005)
<223>  First exon (5'UTR) and first intron

<220>
<221>  misc_feature
<222>  (2022)..(3833)
<223>  GUS coding sequence

<220>
<221>  terminator
<222>  (3843)..(4096)
<223>  NOS

<400>  9
aagcttgcat gccctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag       60

cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag      120

tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa tataatctat      180

agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct      240

aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg      300

tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt      360

acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat      420

tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt      480

aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt      540

aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt      600

taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc      660

caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc      720

gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga      780

cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcacggcagc tacgggggat      840

tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc      900

tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct      960

cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc     1020

cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac     1080

ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta     1140

cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt     1200

ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt     1260

gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt     1320

gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt     1380

gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg atttattaat     1440

tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg     1500

aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag     1560

atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc     1620

tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta     1680

tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg     1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat     1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa     1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt     1920

tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc     1980

ctgttgtttg gtgttacttc tgcaggtcga ctctagagga tatgttacgt cctgtagaaa     2040

ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa     2100

actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg     2160

tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg     2220

tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc     2280

gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc     2340

atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa     2400

gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa     2460

tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact     2520

atgccggaat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata     2580

tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg     2640

tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg     2700

gacaaggcac tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag     2760

gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc     2820

ttcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac     2880

cgttctactt tactggcttt ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg     2940

ataacgtgct gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc     3000

gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg     3060

tggtgattga tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag     3120

cgggcaacaa gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag     3180

cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga     3240

tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc     3300

cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa     3360

tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc     3420

gttattacgg atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa     3480

aagaacttct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg     3540

tggatacgtt agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt     3600

gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac     3660

aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca     3720

agaaagggat cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac     3780

gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgacgagctc     3840

gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg     3900

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc     3960

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac     4020

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct     4080

atgttactag atcggggaat tc                                              4102

<210>  10
<211>  3027
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette Ubi-EYFP-NOS

<220>
<221>  promoter
<222>  (1)..(995)
<223>  Ubi Promoter

<220>
<221>  misc_feature
<222>  (996)..(2005)
<223>  First exon (5'UTR) and first intron

<220>
<221>  misc_feature
<222>  (2030)..(2758)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2768)..(3021)
<223>  NOS

<400>  10
aagcttgcat gccctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag       60

cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag      120

tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa tataatctat      180

agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct      240

aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg      300

tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt      360

acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat      420

tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt      480

aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt      540

aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt      600

taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc      660

caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc      720

gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga      780

cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcacggcagc tacgggggat      840

tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc      900

tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct      960

cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc     1020

cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac     1080

ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta     1140

cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt     1200

ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt     1260

gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt     1320

gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt     1380

gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg atttattaat     1440

tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg     1500

aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag     1560

atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc     1620

tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta     1680

tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg     1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat     1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa     1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt     1920

tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc     1980

ctgttgtttg gtgttacttc tgcagtgcag gtcgactcta gaggatccca tggtgagcaa     2040

gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa     2100

cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac     2160

cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac     2220

cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt     2280

cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga     2340

cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat     2400

cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta     2460

caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt     2520

gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca     2580

gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta     2640

ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt     2700

cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagagat ctatctagcg     2760

agctcgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc     2820

ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt     2880

aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt     2940

aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt     3000

catctatgtt actagatcgg ggaattc                                         3027

<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P-2 PCR primer

<400>  11
acgcggtaac acgtagtcct aaggt                                             25

<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P-W3F PCR primer

<400>  12
gacatcaaat ggttgtatcc                                                   20

<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P-W4F PCR primer

<400>  13
acaccgcatt cagagtgaag                                                   20

<210>  14
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P-W1R PCR primer

<400>  14
ccgcattaac gttctcc                                                      17

<210>  15
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCBV/Prom/F PCR primer

<400>  15
gaagaacagc atgctgaaca tctgtggaag atgc                                   34

<210>  16
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCBV/Prom/R PCR pr

<400>  16
caaacttgct caaatgatca tgtggtgaac taccgatg                               38

<210>  17
<211>  1816
<212>  DNA
<213>  Sugarcane bacilliform virus

<220>
<221>  promoter
<222>  (1)..(1786)
<223>  SCBV21 Promoter

<220>
<221>  misc_signal
<222>  (1787)..(1787)
<223>  Transcription start site

<220>
<221>  misc_feature
<222>  (1813)..(1816)
<223>  n is a, c, t, or g

<400>  17
gaagaacagc atgctgaaca tctgtggaag atgctacaga tatgcaagaa gaatgggtta       60

atcttaagcc cttccaagta taaattggag taaaaagagt tgactttctt ggttcaacaa      120

ttggagataa tcagttagct gttcaagaac atatagtctc caagatagct gattttgatg      180

aagaacgtct caagaccaag gaaggactga aaagctggct ggcaacactc aattatgcca      240

gaaatcacat caaggatatg ggaaaactcc ttggaccctt atatcctaaa acttcagaaa      300

agggagcaaa aggattaaat tctgaagatt ggaaattaat cagcagaatc aagacaatgg      360

tcagaaatct gccaaatctg actattccac cagaggatgc atatattatc attgaaacag      420

atgcttgtgc aactggttgg ggtgcagttt gcaaatggaa gaaatccaag gcagacccaa      480

gaagctccga gctcatatgt cgatatgcaa gtgggaaatt tgacaaacca aaagggacat      540

gtgatgcaga aatctatgga gtaatgaatg ggctggagaa aatgagactc ttttatcttg      600

ataaaaggga aatcactgtg aggacagata gtgccgcaat agagaggttc tacaacaaga      660

gtgttgaaca taaaccctca gaaatccgtt ggataaggtt tatggactat atcactggag      720

caggaccaaa gattgtgatt gagcatatca aaggaaaaca caatggtctg gcagatatcc      780

tctcaagatt gaaagcaaaa ctggcagaat caccttcaga agaagtggtt ttacttgcga      840

aagctttaaa ggaagttgca tactatcctg aacacccgca agtgccaaaa ctaattgaat      900

ggggaaagca aattcttgat ccatttccca agttcaagaa ggacatgttt gaaaaaactg      960

aacacatcat gatggctagt caagagccta cactgctttg tggatgtaga aggcctgcat     1020

atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa tgtgcaatga     1080

acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa gaacgaattg     1140

aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta ccaagtacat     1200

caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag gataaagaag     1260

ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt gacagaccaa     1320

ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg cagaagatca     1380

aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt gagttctggt     1440

tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca gttgatctta     1500

ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac ggaaggacaa     1560

ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt tcggcgccag     1620

caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct tacctttgtc     1680

ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt gtgtgttccc     1740

tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc accacatgat     1800

catttgagca agnnnn                                                     1816

<210>  18
<211>  1825
<212>  DNA
<213>  Sugarcane bacilliform virus

<220>
<221>  promoter
<222>  (1)..(1786)
<223>  SCBV21 Promoter

<220>
<221>  misc_signal
<222>  (1787)..(1787)
<223>  Transcription start site

<220>
<221>  variation
<222>  (1787)..(1787)
<223>  from 1 to 40 nucleotides, each of which may be c or a or g or t

<220>
<221>  misc_feature
<222>  (1817)..(1821)
<223>  n is a, c, t, or g

<220>
<221>  misc_feature
<222>  (1822)..(1824)
<223>  Start codon

<220>
<221>  misc_feature
<222>  (1825)..(1825)
<223>  n is g or t

<400>  18
gaagaacagc atgctgaaca tctgtggaag atgctacaga tatgcaagaa gaatgggtta       60

atcttaagcc cttccaagta taaattggag taaaaagagt tgactttctt ggttcaacaa      120

ttggagataa tcagttagct gttcaagaac atatagtctc caagatagct gattttgatg      180

aagaacgtct caagaccaag gaaggactga aaagctggct ggcaacactc aattatgcca      240

gaaatcacat caaggatatg ggaaaactcc ttggaccctt atatcctaaa acttcagaaa      300

agggagcaaa aggattaaat tctgaagatt ggaaattaat cagcagaatc aagacaatgg      360

tcagaaatct gccaaatctg actattccac cagaggatgc atatattatc attgaaacag      420

atgcttgtgc aactggttgg ggtgcagttt gcaaatggaa gaaatccaag gcagacccaa      480

gaagctccga gctcatatgt cgatatgcaa gtgggaaatt tgacaaacca aaagggacat      540

gtgatgcaga aatctatgga gtaatgaatg ggctggagaa aatgagactc ttttatcttg      600

ataaaaggga aatcactgtg aggacagata gtgccgcaat agagaggttc tacaacaaga      660

gtgttgaaca taaaccctca gaaatccgtt ggataaggtt tatggactat atcactggag      720

caggaccaaa gattgtgatt gagcatatca aaggaaaaca caatggtctg gcagatatcc      780

tctcaagatt gaaagcaaaa ctggcagaat caccttcaga agaagtggtt ttacttgcga      840

aagctttaaa ggaagttgca tactatcctg aacacccgca agtgccaaaa ctaattgaat      900

ggggaaagca aattcttgat ccatttccca agttcaagaa ggacatgttt gaaaaaactg      960

aacacatcat gatggctagt caagagccta cactgctttg tggatgtaga aggcctgcat     1020

atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa tgtgcaatga     1080

acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa gaacgaattg     1140

aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta ccaagtacat     1200

caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag gataaagaag     1260

ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt gacagaccaa     1320

ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg cagaagatca     1380

aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt gagttctggt     1440

tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca gttgatctta     1500

ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac ggaaggacaa     1560

ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt tcggcgccag     1620

caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct tacctttgtc     1680

ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt gtgtgttccc     1740

tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc accacatgat     1800

catttgagca agtttgnnnn natgn                                           1825

<210>  19
<211>  5787
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSK-SCBV21-EYFP-NOS Vector

<220>
<221>  promoter
<222>  (710)..(2525)
<223>  SCBV21 Promoter

<220>
<221>  misc_signal
<222>  (1764)..(1813)
<223>  Transcription start site TSS1

<220>
<221>  misc_signal
<222>  (2446)..(2495)
<223>  Transcription start site TSS2

<220>
<221>  misc_feature
<222>  (2547)..(3275)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (3285)..(3538)
<223>  NOS

<400>  19
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg      660

gccccccctc gaggtcgacc tgcaggcggc cgcgaattca ctagtgattg aagaacagca      720

tgctgaacat ctgtggaaga tgctacagat atgcaagaag aatgggttaa tcttaagccc      780

ttccaagtat aaattggagt aaaaagagtt gactttcttg gttcaacaat tggagataat      840

cagttagctg ttcaagaaca tatagtctcc aagatagctg attttgatga agaacgtctc      900

aagaccaagg aaggactgaa aagctggctg gcaacactca attatgccag aaatcacatc      960

aaggatatgg gaaaactcct tggaccctta tatcctaaaa cttcagaaaa gggagcaaaa     1020

ggattaaatt ctgaagattg gaaattaatc agcagaatca agacaatggt cagaaatctg     1080

ccaaatctga ctattccacc agaggatgca tatattatca ttgaaacaga tgcttgtgca     1140

actggttggg gtgcagtttg caaatggaag aaatccaagg cagacccaag aagctccgag     1200

ctcatatgtc gatatgcaag tgggaaattt gacaaaccaa aagggacatg tgatgcagaa     1260

atctatggag taatgaatgg gctggagaaa atgagactct tttatcttga taaaagggaa     1320

atcactgtga ggacagatag tgccgcaata gagaggttct acaacaagag tgttgaacat     1380

aaaccctcag aaatccgttg gataaggttt atggactata tcactggagc aggaccaaag     1440

attgtgattg agcatatcaa aggaaaacac aatggtctgg cagatatcct ctcaagattg     1500

aaagcaaaac tggcagaatc accttcagaa gaagtggttt tacttgcgaa agctttaaag     1560

gaagttgcat actatcctga acacccgcaa gtgccaaaac taattgaatg gggaaagcaa     1620

attcttgatc catttcccaa gttcaagaag gacatgtttg aaaaaactga acacatcatg     1680

atggctagtc aagagcctac actgctttgt ggatgtagaa ggcctgcata tcagttcaca     1740

tctggcacaa aactcaaccc aagcaggaag ttctataaat gtgcaatgaa catgtgccac     1800

tgctggtatt gggcagatct tttagaagaa tatgtccaag aacgaattga agtgttcatg     1860

attgagaact ttgacaagaa aatgggaatt caagatgtac caagtacatc aaatgctaac     1920

attccaggaa attttaaatc tcttgcagat ttgaagaagg ataaagaagc taaagctgaa     1980

tatcaagaca tgcttgataa tcatcgttca agcattattg acagaccaag gccaacagat     2040

gaacacttca agcctggata catgtacacc gattccctgc agaagatcaa ggaggactac     2100

gcaagcccaa gacaggagga accaccatga gaagacattg agttctggtt atgcaaggaa     2160

gaagactacc acacagaaga cctcaataca gaagatgcag ttgatcttac tgacgtaagc     2220

aatgacgatc agtggaggcg atcgtaagca atgatgcacg gaaggacaat tatggagcgt     2280

ggaggaccca tcaagcactc agaacgcgaa cctcaacttt cggcgccagc accttgtatc     2340

tttagttggt gtgtgtcttt ttcggcatct gtgccacctt acctttgtcg gccacgttgc     2400

ctatgcttag cacctacgca agcatagcgc tcggctggtg tgtgttccct ctgcctatat     2460

aaggcatggt tgtaagactc ttacactcat cggtagttca ccacatgatc atttgagcaa     2520

gtttgaatcg aattcccgcg gccgccatgg tgagcaaggg cgaggagctg ttcaccgggg     2580

tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg     2640

gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg     2700

gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct     2760

tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag     2820

gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg     2880

aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca     2940

aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct     3000

atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca     3060

tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg     3120

gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc     3180

ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc     3240

tcggcatgga cgagctgtac aagagatcta tctagcgagc tcgatcgttc aaacatttgg     3300

caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt     3360

ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga     3420

tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata     3480

tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggga     3540

tgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc     3600

tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa     3660

attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct     3720

ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc     3780

agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg     3840

gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     3900

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     3960

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     4020

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     4080

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     4140

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     4200

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt     4260

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     4320

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     4380

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     4440

agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg     4500

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     4560

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     4620

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact     4680

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa     4740

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt     4800

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     4860

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     4920

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc     4980

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     5040

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     5100

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     5160

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     5220

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     5280

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     5340

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     5400

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     5460

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     5520

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     5580

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     5640

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     5700

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     5760

cgcgcacatt tccccgaaaa gtgccac                                         5787

<210>  20
<211>  4964
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSK-SCBV21-EYFP-NOS Vector - deletion B

<220>
<221>  promoter
<222>  (710)..(1722)
<223>  SCBV21 Promoter deletion B

<220>
<221>  misc_feature
<222>  (1724)..(2452)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2462)..(2715)
<223>  NOS

<400>  20
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg      660

gccccccctc gaggtcgacc tgcaggcggc cgcgaattca ctagtgattg aagaacagca      720

tgctgaacat ctgtggaaga tgctacagat atgcaagaag aatgggttaa tcttaagccc      780

ttccaagtat aaattggagt aaaaagagtt gactttcttg gttcaacaat tggagataat      840

cagttagctg ttcaagaaca tatagtctcc aagatagctg attttgatga agaacgtctc      900

aagaccaagg aaggactgaa aagctggctg gcaacactca attatgccag aaatcacatc      960

aaggatatgg gaaaactcct tggaccctta tatcctaaaa cttcagaaaa gggagcaaaa     1020

ggattaaatt ctgaagattg gaaattaatc agcagaatca agacaatggt cagaaatctg     1080

ccaaatctga ctattccacc agaggatgca tatattatca ttgaaacaga tgcttgtgca     1140

actggttggg gtgcagtttg caaatggaag aaatccaagg cagacccaag aagctccgag     1200

ctcatatgtc gatatgcaag tgggaaattt gacaaaccaa aagggacatg tgatgcagaa     1260

atctatggag taatgaatgg gctggagaaa atgagactct tttatcttga taaaagggaa     1320

atcactgtga ggacagatag tgccgcaata gagaggttct acaacaagag tgttgaacat     1380

aaaccctcag aaatccgttg gataaggttt atggactata tcactggagc aggaccaaag     1440

attgtgattg agcatatcaa aggaaaacac aatggtctgg cagatatcct ctcaagattg     1500

aaagcaaaac tggcagaatc accttcagaa gaagtggttt tacttgcgaa agctttaaag     1560

gaagttgcat actatcctga acacccgcaa gtgccaaaac taattgaatg gggaaagcaa     1620

attcttgatc catttcccaa gttcaagaag gacatgtttg aaaaaactga acacatcatg     1680

atggctagtc aagagcctac actgctttgt ggatgtagaa ggcatggtga gcaagggcga     1740

ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca     1800

caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa     1860

gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg     1920

ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa     1980

gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa     2040

ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct     2100

gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta     2160

caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt     2220

caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa     2280

cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc     2340

cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac     2400

cgccgccggg atcactctcg gcatggacga gctgtacaag agatctatct agcgagctcg     2460

atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga     2520

tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca     2580

tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg     2640

cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta     2700

tgttactaga tcggggatgg gggatccact agttctagag cggccgccac cgcggtggag     2760

ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata     2820

gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag     2880

cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg     2940

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca     3000

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     3060

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     3120

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     3180

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3240

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3300

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3360

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     3420

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     3480

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     3540

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     3600

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     3660

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     3720

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     3780

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     3840

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     3900

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     3960

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4020

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     4080

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     4140

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     4200

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     4260

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     4320

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     4380

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     4440

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     4500

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     4560

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     4620

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     4680

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     4740

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     4800

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     4860

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     4920

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                      4964

<210>  21
<211>  4721
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSK-SCBV21-EYFP-NOS Vector - deletion C

<220>
<221>  promoter
<222>  (674)..(1478)
<223>  SCBV21 Promoter deletion C

<220>
<221>  misc_signal
<222>  (718)..(767)
<223>  Transcription start site TSS1

<220>
<221>  misc_signal
<222>  (1400)..(1449)
<223>  Transcription start site TSS2

<220>
<221>  misc_feature
<222>  (1481)..(2209)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2219)..(2472)
<223>  NOS

<400>  21
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg      660

gccccccctc gagaggcctg catatcagtt cacatctggc acaaaactca acccaagcag      720

gaagttctat aaatgtgcaa tgaacatgtg ccactgctgg tattgggcag atcttttaga      780

agaatatgtc caagaacgaa ttgaagtgtt catgattgag aactttgaca agaaaatggg      840

aattcaagat gtaccaagta catcaaatgc taacattcca ggaaatttta aatctcttgc      900

agatttgaag aaggataaag aagctaaagc tgaatatcaa gacatgcttg ataatcatcg      960

ttcaagcatt attgacagac caaggccaac agatgaacac ttcaagcctg gatacatgta     1020

caccgattcc ctgcagaaga tcaaggagga ctacgcaagc ccaagacagg aggaaccacc     1080

atgagaagac attgagttct ggttatgcaa ggaagaagac taccacacag aagacctcaa     1140

tacagaagat gcagttgatc ttactgacgt aagcaatgac gatcagtgga ggcgatcgta     1200

agcaatgatg cacggaagga caattatgga gcgtggagga cccatcaagc actcagaacg     1260

cgaacctcaa ctttcggcgc cagcaccttg tatctttagt tggtgtgtgt ctttttcggc     1320

atctgtgcca ccttaccttt gtcggccacg ttgcctatgc ttagcaccta cgcaagcata     1380

gcgctcggct ggtgtgtgtt ccctctgcct atataaggca tggttgtaag actcttacac     1440

tcatcggtag ttcaccacat gatcatttga gcaagtttcc atggtgagca agggcgagga     1500

gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa     1560

gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt     1620

catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta     1680

cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc     1740

cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta     1800

caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa     1860

gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa     1920

cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa     1980

gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac     2040

ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc     2100

cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc     2160

cgccgggatc actctcggca tggacgagct gtacaagaga tctatctagc gagctcgatc     2220

gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga     2280

ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga     2340

cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga     2400

tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt     2460

tactagatcg gggatggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc     2520

cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct     2580

gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat     2640

aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc     2700

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg     2760

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct     2820

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     2880

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     2940

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     3000

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     3060

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     3120

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     3180

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     3240

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     3300

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     3360

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     3420

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     3480

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     3540

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     3600

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     3660

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     3720

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     3780

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     3840

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     3900

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     3960

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     4020

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     4080

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     4140

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     4200

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     4260

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     4320

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     4380

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     4440

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     4500

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     4560

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     4620

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa     4680

acaaataggg gttccgcgca catttccccg aaaagtgcca c                         4721

<210>  22
<211>  4626
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSK-SCBV21-EYFP-NOS Vector - deletion D

<220>
<221>  promoter
<222>  (674)..(1383)
<223>  SCBV21 Promoter deletion D

<220>
<221>  misc_signal
<222>  (1305)..(1354)
<223>  Transcription start site TSS2

<220>
<221>  misc_feature
<222>  (1386)..(2114)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2124)..(2377)
<223>  NOS

<400>  22
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg      660

gccccccctc gagagatctt ttagaagaat atgtccaaga acgaattgaa gtgttcatga      720

ttgagaactt tgacaagaaa atgggaattc aagatgtacc aagtacatca aatgctaaca      780

ttccaggaaa ttttaaatct cttgcagatt tgaagaagga taaagaagct aaagctgaat      840

atcaagacat gcttgataat catcgttcaa gcattattga cagaccaagg ccaacagatg      900

aacacttcaa gcctggatac atgtacaccg attccctgca gaagatcaag gaggactacg      960

caagcccaag acaggaggaa ccaccatgag aagacattga gttctggtta tgcaaggaag     1020

aagactacca cacagaagac ctcaatacag aagatgcagt tgatcttact gacgtaagca     1080

atgacgatca gtggaggcga tcgtaagcaa tgatgcacgg aaggacaatt atggagcgtg     1140

gaggacccat caagcactca gaacgcgaac ctcaactttc ggcgccagca ccttgtatct     1200

ttagttggtg tgtgtctttt tcggcatctg tgccacctta cctttgtcgg ccacgttgcc     1260

tatgcttagc acctacgcaa gcatagcgct cggctggtgt gtgttccctc tgcctatata     1320

aggcatggtt gtaagactct tacactcatc ggtagttcac cacatgatca tttgagcaag     1380

tttccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc     1440

tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca     1500

cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc     1560

ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca     1620

tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca     1680

tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca     1740

ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg     1800

ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga     1860

agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc     1920

tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca     1980

accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca     2040

tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca     2100

agagatctat ctagcgagct cgatcgttca aacatttggc aataaagttt cttaagattg     2160

aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat     2220

gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc     2280

ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa     2340

ttatcgcgcg cggtgtcatc tatgttacta gatcggggat gggggatcca ctagttctag     2400

agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc     2460

gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc     2520

cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct     2580

aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc     2640

agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt     2700

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     2760

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     2820

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     2880

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     2940

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     3000

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     3060

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     3120

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     3180

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     3240

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     3300

actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct     3360

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     3420

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     3480

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     3540

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     3600

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     3660

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     3720

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     3780

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     3840

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     3900

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca     3960

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     4020

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     4080

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     4140

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     4200

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg     4260

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     4320

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     4380

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     4440

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     4500

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     4560

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     4620

tgccac                                                                4626

<210>  23
<211>  4637
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSK-SCBV21-EYFP-NOS Vector - deletion E

<220>
<221>  promoter
<222>  (674)..(1394)
<223>  SCBV21 Promoter deletion E

<220>
<221>  misc_signal
<222>  (718)..(767)
<223>  Transcription start site TSS1

<220>
<221>  misc_feature
<222>  (1397)..(2125)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2135)..(2388)
<223>  NOS

<400>  23
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg      660

gccccccctc gagaggcctg catatcagtt cacatctggc acaaaactca acccaagcag      720

gaagttctat aaatgtgcaa tgaacatgtg ccactgctgg tattgggcag atcttttaga      780

agaatatgtc caagaacgaa ttgaagtgtt catgattgag aactttgaca agaaaatggg      840

aattcaagat gtaccaagta catcaaatgc taacattcca ggaaatttta aatctcttgc      900

agatttgaag aaggataaag aagctaaagc tgaatatcaa gacatgcttg ataatcatcg      960

ttcaagcatt attgacagac caaggccaac agatgaacac ttcaagcctg gatacatgta     1020

caccgattcc ctgcagaaga tcaaggagga ctacgcaagc ccaagacagg aggaaccacc     1080

atgagaagac attgagttct ggttatgcaa ggaagaagac taccacacag aagacctcaa     1140

tacagaagat gcagttgatc ttactgacgt aagcaatgac gatcagtgga ggcgatcgta     1200

agcaatgatg cacggaagga caattatgga gcgtggagga cccatcaagc actcagaacg     1260

cgaacctcaa ctttcggcgc cagcaccttg tatctttagt tggtgtgtgt ctttttcggc     1320

atctgtgcca ccttaccttt gtcggccacg ttgcctatgc ttagcaccta cgcaagcata     1380

gcgctcggct ggtgccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat     1440

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga     1500

gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc     1560

cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta     1620

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca     1680

ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     1740

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     1800

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     1860

cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg     1920

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     1980

gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa     2040

gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga     2100

cgagctgtac aagagatcta tctagcgagc tcgatcgttc aaacatttgg caataaagtt     2160

tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt     2220

acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta     2280

tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa     2340

actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggga tgggggatcc     2400

actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg     2460

gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc     2520

gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta     2580

atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa     2640

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     2700

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     2760

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     2820

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     2880

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     2940

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     3000

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     3060

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt     3120

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     3180

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     3240

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     3300

gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa     3360

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     3420

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     3480

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     3540

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     3600

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt     3660

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact     3720

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat     3780

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg     3840

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg     3900

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat     3960

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc     4020

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt     4080

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc     4140

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga     4200

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc     4260

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa     4320

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta     4380

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg     4440

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg     4500

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat     4560

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt     4620

tccccgaaaa gtgccac                                                    4637

<210>  24
<211>  4542
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSK-SCBV21-EYFP-NOS Vector - deletion F

<220>
<221>  promoter
<222>  (674)..(1299)
<223>  SCBV21 Promoter deletion F

<220>
<221>  misc_feature
<222>  (1302)..(2030)
<223>  EYFP coding sequence

<220>
<221>  terminator
<222>  (2040)..(2293)
<223>  NOS

<400>  24
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg      660

gccccccctc gagagatctt ttagaagaat atgtccaaga acgaattgaa gtgttcatga      720

ttgagaactt tgacaagaaa atgggaattc aagatgtacc aagtacatca aatgctaaca      780

ttccaggaaa ttttaaatct cttgcagatt tgaagaagga taaagaagct aaagctgaat      840

atcaagacat gcttgataat catcgttcaa gcattattga cagaccaagg ccaacagatg      900

aacacttcaa gcctggatac atgtacaccg attccctgca gaagatcaag gaggactacg      960

caagcccaag acaggaggaa ccaccatgag aagacattga gttctggtta tgcaaggaag     1020

aagactacca cacagaagac ctcaatacag aagatgcagt tgatcttact gacgtaagca     1080

atgacgatca gtggaggcga tcgtaagcaa tgatgcacgg aaggacaatt atggagcgtg     1140

gaggacccat caagcactca gaacgcgaac ctcaactttc ggcgccagca ccttgtatct     1200

ttagttggtg tgtgtctttt tcggcatctg tgccacctta cctttgtcgg ccacgttgcc     1260

tatgcttagc acctacgcaa gcatagcgct cggctggtgc catggtgagc aagggcgagg     1320

agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca     1380

agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt     1440

tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct     1500

acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt     1560

ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact     1620

acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga     1680

agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca     1740

acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca     1800

agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca     1860

cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg     1920

ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg     1980

ccgccgggat cactctcggc atggacgagc tgtacaagag atctatctag cgagctcgat     2040

cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg     2100

attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg     2160

acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg     2220

atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg     2280

ttactagatc ggggatgggg gatccactag ttctagagcg gccgccaccg cggtggagct     2340

ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc     2400

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca     2460

taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct     2520

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac     2580

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc     2640

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt     2700

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg     2760

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     2820

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     2880

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     2940

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     3000

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     3060

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     3120

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     3180

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag     3240

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     3300

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     3360

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc     3420

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca     3480

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa     3540

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat     3600

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct     3660

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt     3720

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat     3780

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta     3840

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg     3900

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt     3960

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg     4020

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg     4080

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc     4140

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa     4200

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac     4260

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt     4320

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg     4380

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa     4440

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata     4500

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac                        4542

<210>  25
<211>  1018
<212>  DNA
<213>  Sugarcane bacilliform virus

<400>  25
gaagaacagc atgctgaaca tctgtggaag atgctacaga tatgcaagaa gaatgggtta       60

atcttaagcc cttccaagta taaattggag taaaaagagt tgactttctt ggttcaacaa      120

ttggagataa tcagttagct gttcaagaac atatagtctc caagatagct gattttgatg      180

aagaacgtct caagaccaag gaaggactga aaagctggct ggcaacactc aattatgcca      240

gaaatcacat caaggatatg ggaaaactcc ttggaccctt atatcctaaa acttcagaaa      300

agggagcaaa aggattaaat tctgaagatt ggaaattaat cagcagaatc aagacaatgg      360

tcagaaatct gccaaatctg actattccac cagaggatgc atatattatc attgaaacag      420

atgcttgtgc aactggttgg ggtgcagttt gcaaatggaa gaaatccaag gcagacccaa      480

gaagctccga gctcatatgt cgatatgcaa gtgggaaatt tgacaaacca aaagggacat      540

gtgatgcaga aatctatgga gtaatgaatg ggctggagaa aatgagactc ttttatcttg      600

ataaaaggga aatcactgtg aggacagata gtgccgcaat agagaggttc tacaacaaga      660

gtgttgaaca taaaccctca gaaatccgtt ggataaggtt tatggactat atcactggag      720

caggaccaaa gattgtgatt gagcatatca aaggaaaaca caatggtctg gcagatatcc      780

tctcaagatt gaaagcaaaa ctggcagaat caccttcaga agaagtggtt ttacttgcga      840

aagctttaaa ggaagttgca tactatcctg aacacccgca agtgccaaaa ctaattgaat      900

ggggaaagca aattcttgat ccatttccca agttcaagaa ggacatgttt gaaaaaactg      960

aacacatcat gatggctagt caagagccta cactgctttg tggatgtaga aggcatgg       1018

<210>  26
<211>  811
<212>  DNA
<213>  Sugarcane bacilliform virus

<400>  26
aggcctgcat atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa       60

tgtgcaatga acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa      120

gaacgaattg aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta      180

ccaagtacat caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag      240

gataaagaag ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt      300

gacagaccaa ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg      360

cagaagatca aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt      420

gagttctggt tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca      480

gttgatctta ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac      540

ggaaggacaa ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt      600

tcggcgccag caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct      660

tacctttgtc ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt      720

gtgtgttccc tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc      780

accacatgat catttgagca agtttccatg g                                     811

<210>  27
<211>  716
<212>  DNA
<213>  Sugarcane bacilliform virus

<400>  27
agatctttta gaagaatatg tccaagaacg aattgaagtg ttcatgattg agaactttga       60

caagaaaatg ggaattcaag atgtaccaag tacatcaaat gctaacattc caggaaattt      120

taaatctctt gcagatttga agaaggataa agaagctaaa gctgaatatc aagacatgct      180

tgataatcat cgttcaagca ttattgacag accaaggcca acagatgaac acttcaagcc      240

tggatacatg tacaccgatt ccctgcagaa gatcaaggag gactacgcaa gcccaagaca      300

ggaggaacca ccatgagaag acattgagtt ctggttatgc aaggaagaag actaccacac      360

agaagacctc aatacagaag atgcagttga tcttactgac gtaagcaatg acgatcagtg      420

gaggcgatcg taagcaatga tgcacggaag gacaattatg gagcgtggag gacccatcaa      480

gcactcagaa cgcgaacctc aactttcggc gccagcacct tgtatcttta gttggtgtgt      540

gtctttttcg gcatctgtgc caccttacct ttgtcggcca cgttgcctat gcttagcacc      600

tacgcaagca tagcgctcgg ctggtgtgtg ttccctctgc ctatataagg catggttgta      660

agactcttac actcatcggt agttcaccac atgatcattt gagcaagttt ccatgg          716

<210>  28
<211>  727
<212>  DNA
<213>  Sugarcane bacilliform virus

<400>  28
aggcctgcat atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa       60

tgtgcaatga acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa      120

gaacgaattg aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta      180

ccaagtacat caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag      240

gataaagaag ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt      300

gacagaccaa ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg      360

cagaagatca aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt      420

gagttctggt tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca      480

gttgatctta ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac      540

ggaaggacaa ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt      600

tcggcgccag caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct      660

tacctttgtc ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt      720

gccatgg                                                                727

<210>  29
<211>  632
<212>  DNA
<213>  Sugarcane bacilliform virus

<400>  29
agatctttta gaagaatatg tccaagaacg aattgaagtg ttcatgattg agaactttga       60

caagaaaatg ggaattcaag atgtaccaag tacatcaaat gctaacattc caggaaattt      120

taaatctctt gcagatttga agaaggataa agaagctaaa gctgaatatc aagacatgct      180

tgataatcat cgttcaagca ttattgacag accaaggcca acagatgaac acttcaagcc      240

tggatacatg tacaccgatt ccctgcagaa gatcaaggag gactacgcaa gcccaagaca      300

ggaggaacca ccatgagaag acattgagtt ctggttatgc aaggaagaag actaccacac      360

agaagacctc aatacagaag atgcagttga tcttactgac gtaagcaatg acgatcagtg      420

gaggcgatcg taagcaatga tgcacggaag gacaattatg gagcgtggag gacccatcaa      480

gcactcagaa cgcgaacctc aactttcggc gccagcacct tgtatcttta gttggtgtgt      540

gtctttttcg gcatctgtgc caccttacct ttgtcggcca cgttgcctat gcttagcacc      600

tacgcaagca tagcgctcgg ctggtgccat gg                                    632

<210>  30
<211>  50
<212>  DNA
<213>  Sugarcane bacilliform virus

<220>
<221>  misc_signal
<222>  (41)..(41)
<223>  Transcription start site

<400>  30
caggaagttc tataaatgtg caatgaacat gtgccactgc tggtattggg                  50

<210>  31
<211>  50
<212>  DNA
<213>  Sugarcane bacilliform virus

<220>
<221>  misc_signal
<222>  (41)..(41)
<223>  Transcription start site

<400>  31
tccctctgcc tatataaggc atggttgtaa gactcttaca ctcatcggta                  50

<210>  32
<211>  814
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCBV21 Promoter with TSS1 and TSS2

<220>
<221>  misc_signal
<222>  (45)..(94)
<223>  Transcription start site TSS1

<220>
<221>  misc_signal
<222>  (727)..(776)
<223>  Transcription start site TSS2

<220>
<221>  variation
<222>  (806)..(806)
<223>  from 1 to 40 nucleotides, each of which may be c or a or g or t

<220>
<221>  misc_feature
<222>  (806)..(806)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (807)..(810)
<223>  n is a, t, c, or g

<220>
<221>  misc_feature
<222>  (811)..(813)
<223>  Start codon

<220>
<221>  misc_feature
<222>  (814)..(814)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1814)..(1814)
<223>  n is g or t

<400>  32
aggcctgcat atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa       60

tgtgcaatga acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa      120

gaacgaattg aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta      180

ccaagtacat caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag      240

gataaagaag ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt      300

gacagaccaa ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg      360

cagaagatca aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt      420

gagttctggt tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca      480

gttgatctta ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac      540

ggaaggacaa ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt      600

tcggcgccag caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct      660

tacctttgtc ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt      720

gtgtgttccc tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc      780

accacatgat catttgagca agtttnnnnn atgn                                  814

<210>  33
<211>  719
<212>  DNA
<213>  Sugarcane bacilliform virus

<220>
<221>  misc_signal
<222>  (632)..(681)
<223>  Transcription start site TSS2

<220>
<221>  misc_feature
<222>  (711)..(715)
<223>  n is a, t, c, or g

<220>
<221>  misc_feature
<222>  (716)..(718)
<223>  Start codon

<220>
<221>  variation
<222>  (719)..(719)
<223>  from 1 to 40 nucleotides, each of which may be c or a or g or t

<220>
<221>  misc_feature
<222>  (719)..(719)
<223>  n is g or t

<400>  33
agatctttta gaagaatatg tccaagaacg aattgaagtg ttcatgattg agaactttga       60

caagaaaatg ggaattcaag atgtaccaag tacatcaaat gctaacattc caggaaattt      120

taaatctctt gcagatttga agaaggataa agaagctaaa gctgaatatc aagacatgct      180

tgataatcat cgttcaagca ttattgacag accaaggcca acagatgaac acttcaagcc      240

tggatacatg tacaccgatt ccctgcagaa gatcaaggag gactacgcaa gcccaagaca      300

ggaggaacca ccatgagaag acattgagtt ctggttatgc aaggaagaag actaccacac      360

agaagacctc aatacagaag atgcagttga tcttactgac gtaagcaatg acgatcagtg      420

gaggcgatcg taagcaatga tgcacggaag gacaattatg gagcgtggag gacccatcaa      480

gcactcagaa cgcgaacctc aactttcggc gccagcacct tgtatcttta gttggtgtgt      540

gtctttttcg gcatctgtgc caccttacct ttgtcggcca cgttgcctat gcttagcacc      600

tacgcaagca tagcgctcgg ctggtgtgtg ttccctctgc ctatataagg catggttgta      660

agactcttac actcatcggt agttcaccac atgatcattt gagcaagttt nnnnnatgn       719

<210>  34
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer F1

<400>  34
ttactcgagg cctgcatatc agttcacatc tgg                                    33

<210>  35
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR primer F2

<400>  35
ttactcgaga tcttttagaa gaatatgtcc aagaacg                                37

<210>  36
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer R1

<400>  36
ttaccatgga aacttgctca aatgatcatg tggtgaacta cc                          42

<210>  37
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer R2

<400>  37
ttaccatggc accagccgag cgctatgctt gcgtag                                 36

<210>  38
<211>  2855
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SCBV21-BvLz(m)-35S NOS

<220>
<221>  promoter
<222>  (1)..(1816)
<223>  SCBV21 Promoter

<220>
<221>  misc_feature
<222>  (1857)..(2300)
<223>  BvLz(m) coding sequence

<220>
<221>  terminator
<222>  (2375)..(2580)
<223>  35S

<220>
<221>  terminator
<222>  (2603)..(2855)
<223>  NOS

<400>  38
gaagaacagc atgctgaaca tctgtggaag atgctacaga tatgcaagaa gaatgggtta       60

atcttaagcc cttccaagta taaattggag taaaaagagt tgactttctt ggttcaacaa      120

ttggagataa tcagttagct gttcaagaac atatagtctc caagatagct gattttgatg      180

aagaacgtct caagaccaag gaaggactga aaagctggct ggcaacactc aattatgcca      240

gaaatcacat caaggatatg ggaaaactcc ttggaccctt atatcctaaa acttcagaaa      300

agggagcaaa aggattaaat tctgaagatt ggaaattaat cagcagaatc aagacaatgg      360

tcagaaatct gccaaatctg actattccac cagaggatgc atatattatc attgaaacag      420

atgcttgtgc aactggttgg ggtgcagttt gcaaatggaa gaaatccaag gcagacccaa      480

gaagctccga gctcatatgt cgatatgcaa gtgggaaatt tgacaaacca aaagggacat      540

gtgatgcaga aatctatgga gtaatgaatg ggctggagaa aatgagactc ttttatcttg      600

ataaaaggga aatcactgtg aggacagata gtgccgcaat agagaggttc tacaacaaga      660

gtgttgaaca taaaccctca gaaatccgtt ggataaggtt tatggactat atcactggag      720

caggaccaaa gattgtgatt gagcatatca aaggaaaaca caatggtctg gcagatatcc      780

tctcaagatt gaaagcaaaa ctggcagaat caccttcaga agaagtggtt ttacttgcga      840

aagctttaaa ggaagttgca tactatcctg aacacccgca agtgccaaaa ctaattgaat      900

ggggaaagca aattcttgat ccatttccca agttcaagaa ggacatgttt gaaaaaactg      960

aacacatcat gatggctagt caagagccta cactgctttg tggatgtaga aggcctgcat     1020

atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa tgtgcaatga     1080

acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa gaacgaattg     1140

aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta ccaagtacat     1200

caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag gataaagaag     1260

ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt gacagaccaa     1320

ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg cagaagatca     1380

aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt gagttctggt     1440

tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca gttgatctta     1500

ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac ggaaggacaa     1560

ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt tcggcgccag     1620

caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct tacctttgtc     1680

ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt gtgtgttccc     1740

tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc accacatgat     1800

catttgagca agtttgaatc gaattcccgc ggccgccatg catctcggat ccaaacatgg     1860

cggccctggt gatcctgggc ttcctgttcc tgtccgtggc tgtgcagggc aaggtgttcg     1920

aaaggtgcga actggctagg accctgaaga agctgggcct ggatggctac aagggcgtgt     1980

ccctggctaa ctggctgtgc ctgaccaagt gggaatcctc ctacaacacc aaggctacca     2040

actacaaccc atcctccgaa tccaccgact acggcatctt ccagatcaac tccaagtggt     2100

ggtgcaacga tggcaagacc ccaaacgctg tggatggctg ccacgtgtcc tgctccgagc     2160

tgatggaaaa cgatatcgct aaggctgtgg cttgcgctaa gcacatcgtg tccgaacagg     2220

gcatcaccgc ctgggtggct tggaagtccc actgcaggga tcacgatgtg tcctcctacg     2280

tggaaggctg caccctgtga ttcgaattcg gatccccccg ggctgcagga attcgatatc     2340

aagcttatcg ataccgtcga ggggtccgca aaaatcacca gtctctctct acaaatctat     2400

ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt     2460

cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt     2520

gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag     2580

gggccgctcg acgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt     2640

gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca     2700

tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt     2760

cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa     2820

attatcgcgc gcggtgtcat ctatgttact agatc                                2855

<210>  39
<211>  2745
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette MUbi(no hse)-BvLz(m)-35S

<220>
<221>  promoter
<222>  (1)..(1977)
<223>  MUbi (no hse) Promoter

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (671)..(671)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1248)..(1248)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1315)..(1315)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1542)..(1542)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (2032)..(2475)
<223>  BvLz (m) coding sequence

<220>
<221>  terminator
<222>  (2549)..(2745)
<223>  35S

<400>  39
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc       60

attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt      120

gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata      180

gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta      240

aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt      300

gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta      360

catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt      420

ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta      480

ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta      540

agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt      600

aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc      660

aagcgaanca nacggcacgg natctctgtc gctgcctcca ccgttggact tgctccgctg      720

tcggcatcca gaaattgcgt ggcggcaggc agacgtgagc cggcacgagg cggcctcctc      780

ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct      840

tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt      900

gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc      960

ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt     1020

ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt     1080

ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt     1140

tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc     1200

cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggntt ggtttgccct     1260

tttcctttat ttcaatatat gccgtgccac ttgtttgtcg ggtcatcttt tcatngcttt     1320

tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa     1380

ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca     1440

tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat     1500

gttgatgcgg gttttactga tgcatataca gagatgcttt tnttcgcttg gttgtgatga     1560

tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac     1620

tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac     1680

gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta     1740

ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc     1800

tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga     1860

tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt     1920

gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc     1980

gactctagag gatctgatat ctgatcagaa gacaccatct cggatccaaa catggcggcc     2040

ctggtgatcc tgggcttcct gttcctgtcc gtggctgtgc agggcaaggt gttcgaaagg     2100

tgcgaactgg ctaggaccct gaagaagctg ggcctggatg gctacaaggg cgtgtccctg     2160

gctaactggc tgtgcctgac caagtgggaa tcctcctaca acaccaaggc taccaactac     2220

aacccatcct ccgaatccac cgactacggc atcttccaga tcaactccaa gtggtggtgc     2280

aacgatggca agaccccaaa cgctgtggat ggctgccacg tgtcctgctc cgagctgatg     2340

gaaaacgata tcgctaaggc tgtggcttgc gctaagcaca tcgtgtccga acagggcatc     2400

accgcctggg tggcttggaa gtcccactgc agggatcacg atgtgtcctc ctacgtggaa     2460

ggctgcaccc tgtgattcga attcggatcc cccgggctgc aggaattcga tatcaagctt     2520

atcgataccg tcgaggggtc cgcaaaaatc accagtctct ctctacaaat ctatctctct     2580

ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata     2640

gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa     2700

tacttctatc aataaaattt ctaattccta aaaccaaaat ccagt                     2745

<210>  40
<211>  2977
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette MUbi(no hse)-BvLz(m)-3'SrMV UTR-35S

<220>
<221>  promoter
<222>  (1)..(1977)
<223>  MUbi (no hse) Promoter

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (671)..(671)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1248)..(1248)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1315)..(1315)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1542)..(1542)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (2032)..(2475)
<223>  BvLz (m) coding sequence

<220>
<221>  misc_feature
<222>  (2494)..(2720)
<223>  3' Untranslated Region of SrMV 

<220>
<221>  terminator
<222>  (2781)..(2977)
<223>  35S terminator

<400>  40
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc       60

attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt      120

gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata      180

gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta      240

aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt      300

gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta      360

catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt      420

ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta      480

ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta      540

agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt      600

aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc      660

aagcgaanca nacggcacgg natctctgtc gctgcctcca ccgttggact tgctccgctg      720

tcggcatcca gaaattgcgt ggcggcaggc agacgtgagc cggcacgagg cggcctcctc      780

ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct      840

tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt      900

gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc      960

ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt     1020

ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt     1080

ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt     1140

tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc     1200

cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggntt ggtttgccct     1260

tttcctttat ttcaatatat gccgtgccac ttgtttgtcg ggtcatcttt tcatngcttt     1320

tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa     1380

ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca     1440

tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat     1500

gttgatgcgg gttttactga tgcatataca gagatgcttt tnttcgcttg gttgtgatga     1560

tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac     1620

tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac     1680

gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta     1740

ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc     1800

tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga     1860

tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt     1920

gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc     1980

gactctagag gatctgatat ctgatcagaa gacaccatct cggatccaaa catggcggcc     2040

ctggtgatcc tgggcttcct gttcctgtcc gtggctgtgc agggcaaggt gttcgaaagg     2100

tgcgaactgg ctaggaccct gaagaagctg ggcctggatg gctacaaggg cgtgtccctg     2160

gctaactggc tgtgcctgac caagtgggaa tcctcctaca acaccaaggc taccaactac     2220

aacccatcct ccgaatccac cgactacggc atcttccaga tcaactccaa gtggtggtgc     2280

aacgatggca agaccccaaa cgctgtggat ggctgccacg tgtcctgctc cgagctgatg     2340

gaaaacgata tcgctaaggc tgtggcttgc gctaagcaca tcgtgtccga acagggcatc     2400

accgcctggg tggcttggaa gtcccactgc agggatcacg atgtgtcctc ctacgtggaa     2460

ggctgcaccc tgtgattcga attcggatcc cccgatcttc attgcagttt ttaaagtatt     2520

ttatatattt actatttcag tgagggtctc cctccttagt attatatatg tacttcagaa     2580

atagtagtca ttctgcaggg gagtgaggtt cacctccaac cctatggtta ctatttctta     2640

ctagcgtcga actacattac ggacaccctg ttgtgtggtt ctaccacgag tcaggagctg     2700

cgagtattgt agcaagagaa gaattgggct gcaggaattc gatatcaagc ttatcgatac     2760

cgtcgagggg tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt     2820

ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg     2880

ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta     2940

tcaataaaat ttctaattcc taaaaccaaa atccagt                              2977

<210>  41
<211>  3114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette MUbi(no hse)-5'SrMV UTR-BvLz(sc)-3'SrMV 
       UTR-35S

<220>
<221>  promoter
<222>  (1)..(1977)
<223>  MUbi(no hse) Promoter

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (671)..(671)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1248)..(1248)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1315)..(1315)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1542)..(1542)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (2028)..(2168)
<223>  5' Untranslated Region of SrMV

<220>
<221>  misc_feature
<222>  (2169)..(2612)
<223>  BvLz (sc) coding sequence

<220>
<221>  misc_feature
<222>  (2613)..(2847)
<223>  3' Untranslated Region of SrMV

<220>
<221>  terminator
<222>  (2918)..(3114)
<223>  35S

<400>  41
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc       60

attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt      120

gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata      180

gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta      240

aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt      300

gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta      360

catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt      420

ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta      480

ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta      540

agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt      600

aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc      660

aagcgaanca nacggcacgg natctctgtc gctgcctcca ccgttggact tgctccgctg      720

tcggcatcca gaaattgcgt ggcggcaggc agacgtgagc cggcacgagg cggcctcctc      780

ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct      840

tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt      900

gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc      960

ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt     1020

ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt     1080

ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt     1140

tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc     1200

cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggntt ggtttgccct     1260

tttcctttat ttcaatatat gccgtgccac ttgtttgtcg ggtcatcttt tcatngcttt     1320

tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa     1380

ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca     1440

tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat     1500

gttgatgcgg gttttactga tgcatataca gagatgcttt tnttcgcttg gttgtgatga     1560

tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac     1620

tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac     1680

gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta     1740

ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc     1800

tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga     1860

tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt     1920

gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc     1980

gactctagag gatctgatat ctgatcagaa gacaccatct cggatccaaa acaacaagac     2040

tcaacacaac acaacaagac acagcaaagc aacttatatt gcaacgcatc gtcagcacat     2100

tccaaatcga agttcacggt tcaagagcaa ggtgccttga tcgaactctt tggagaattt     2160

cagcaaacat ggcggctctc gtcatcctgg gattcctttt cctgtcggtt gcggtgcaag     2220

gaaaggtttt cgagcgctgc gagcttgccc ggacgctgaa gaaactgggg ctggacggtt     2280

acaagggtgt ttcccttgct aactggctgt gccttaccaa gtgggagtcc tcctacaaca     2340

ccaaggccac gaactataac ccctcctccg agtctaccga ttacgggatc ttccagatta     2400

actccaagtg gtggtgcaac gacggaaaga cccctaacgc ggtggacggt tgccacgtgt     2460

cctgctccga gcttatggag aacgatattg ccaaggcggt tgcgtgcgcc aagcatattg     2520

tgtccgagca gggtatcacc gcgtgggtcg cctggaagag ccactgcagg gaccacgatg     2580

tgagcagcta cgtggagggc tgcaccctct gatgtactga gatcttcatt gcagttttta     2640

aagtatttta tatatttact atttcagtga gggtctccct ccttagtatt atatatgtac     2700

ttcagaaata gtagtcattc tgcaggggag tgaggttcac ctccaaccct atggttacta     2760

tttcttacta gcgtcgaact acattacgga caccctgttg tgtggttcta ccacgagtca     2820

ggagctgcga gtattgtagc aagagaagaa ttcggatccc ccgggctgca ggaattcgat     2880

atcaagctta tcgataccgt cgaggggtcc gcaaaaatca ccagtctctc tctacaaatc     2940

tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg     3000

gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta     3060

tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagt           3114

<210>  42
<211>  3020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette MUbi(no hse)-BvLz(m)-35S NOS

<220>
<221>  promoter
<222>  (1)..(1977)
<223>  MUbi(no hse) Promoter

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (671)..(671)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1248)..(1248)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1315)..(1315)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1542)..(1542)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (2032)..(2475)
<223>  BvLz (m) coding sequence

<220>
<221>  terminator
<222>  (2549)..(2745)
<223>  35S

<220>
<221>  terminator
<222>  (2768)..(3020)
<223>  NOS

<400>  42
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc       60

attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt      120

gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata      180

gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta      240

aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt      300

gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta      360

catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt      420

ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta      480

ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta      540

agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt      600

aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc      660

aagcgaanca nacggcacgg natctctgtc gctgcctcca ccgttggact tgctccgctg      720

tcggcatcca gaaattgcgt ggcggcaggc agacgtgagc cggcacgagg cggcctcctc      780

ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct      840

tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt      900

gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc      960

ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt     1020

ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt     1080

ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt     1140

tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc     1200

cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggntt ggtttgccct     1260

tttcctttat ttcaatatat gccgtgccac ttgtttgtcg ggtcatcttt tcatngcttt     1320

tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa     1380

ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca     1440

tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat     1500

gttgatgcgg gttttactga tgcatataca gagatgcttt tnttcgcttg gttgtgatga     1560

tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac     1620

tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac     1680

gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta     1740

ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc     1800

tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga     1860

tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt     1920

gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc     1980

gactctagag gatctgatat ctgatcagaa gacaccatct cggatccaaa catggcggcc     2040

ctggtgatcc tgggcttcct gttcctgtcc gtggctgtgc agggcaaggt gttcgaaagg     2100

tgcgaactgg ctaggaccct gaagaagctg ggcctggatg gctacaaggg cgtgtccctg     2160

gctaactggc tgtgcctgac caagtgggaa tcctcctaca acaccaaggc taccaactac     2220

aacccatcct ccgaatccac cgactacggc atcttccaga tcaactccaa gtggtggtgc     2280

aacgatggca agaccccaaa cgctgtggat ggctgccacg tgtcctgctc cgagctgatg     2340

gaaaacgata tcgctaaggc tgtggcttgc gctaagcaca tcgtgtccga acagggcatc     2400

accgcctggg tggcttggaa gtcccactgc agggatcacg atgtgtcctc ctacgtggaa     2460

ggctgcaccc tgtgattcga attcggatcc cccgggctgc aggaattcga tatcaagctt     2520

atcgataccg tcgaggggtc cgcaaaaatc accagtctct ctctacaaat ctatctctct     2580

ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata     2640

gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa     2700

tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgggcc gctcgacgaa     2760

tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg     2820

tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat     2880

gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat     2940

ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt     3000

gtcatctatg ttactagatc                                                 3020

<210>  43
<211>  4020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SPRP(no 5'UTR)-5'SrMV UTR-BvLz(sc)-3'SrMV 
       UTR-35S

<220>
<221>  promoter
<222>  (1)..(2900)
<223>  SPRP(no 5'UTR) promoter

<220>
<221>  misc_feature
<222>  (2934)..(3074)
<223>  5' Untranslated Region of SrMV

<220>
<221>  misc_feature
<222>  (3075)..(3518)
<223>  BvLz (sc) coding sequence

<220>
<221>  misc_feature
<222>  (3519)..(3753)
<223>  3' Untranslated Region of SrMV

<220>
<221>  terminator
<222>  (3824)..(4020)
<223>  35S

<400>  43
atatttgcta gagatgttca aagtgatcat ctcagagcaa tatacggaaa gatgtttgaa       60

aaaatacttt cataagtgtt tggtattgac caagcaatga cccgatacat gtatcgccct      120

tagaacaaat atgaccgatg tatacatcgc ccttagttgc tgattagccg atgagttggg      180

tattgttgct tttcagtttt gaattgtgtt tttagattca tgcattattc acttgaaaac      240

agggagcata tattgacact caaattgggc ctctgcctat ttaaataggc ccatgacagt      300

tctggcacaa tctaaggaag actctataaa gcaacatgat aatagtacca tattggtttt      360

cctataggat aagaccaccc ctactcctta taaaataagg ggtatatgac tgattgagtt      420

tccaactatc taatcgataa aaatagatct actacctagt tttacctttt tccaactctc      480

ttgctgtttg ttgcatctct ttgcgagatt catcggcgtt ctaggcggcc ttgctggtcc      540

tagaataaca ctaggcgtgc tcctcgacgg gtcctctcga gaggcattcc caagcttttg      600

cggcgttgaa tgagttcaag atcggcctaa ccgttctcta gatcggttta gtcggtctta      660

gagttgtttg ctacgtgttt aaagttgtgt gcagcctagg gcgagctagc ccttgctgac      720

gtgtgactta gatcacaatc taacgtctaa cagctggctt gactttgcat taaaaaaaaa      780

gagtagtgtg caagtgtgga agcgtcgttt ttttatttga aaaaacaaaa aaatgcgcag      840

tatattaagg gacatcctaa ttaagaggct aagagcaaat gcacaacagt gtactccacg      900

agagattggt cctgtaagtt gtggatgcat gttagcacag gcacaacagg ccagtagttg      960

gggcgtcagt gtcaacgatg tcgggtcggt ggcaggagag ggtgggaaaa catccacgag     1020

cagaaaagga catcgccgtt ggaacaaggg acgagtgcac cactccggcc acgccgtacc     1080

gtacgcctca gaccccgcca cagcgcgttc gctagctgct gctgagcctg atgcctgaac     1140

acggtcgggt cgccaccaca ctgtccatca tctatccgtt cattcatcca cgactgtgct     1200

tgcacagcca tacactgccg aacctagctc gtgtttagat gcaatttttt tttacaaaat     1260

gctcctgtag cactttttta ttgttatttg gaaattagtg tctaatcgtg aactaattag     1320

gcttaaaaga ttcatctcgt gaatttcatc taaactgtgt aattagtttt attttttatc     1380

tatatttaat gcttcatgca tgtgtctaaa gattcgatgt gatgggaaat attgaaaatt     1440

tttgcaaaat tttttgcatc taaactgagc cctacacctc ggtcccagac cgttcgtcga     1500

tgaatctgga caactacctg tccagatttt tattcctata ccaacagttt tttgaatgga     1560

gggagtacga agtcgccagt acgtgtccct tttaacctgt agtgataaaa ctgtgaattt     1620

ctagataaac ttttgggatg aggccctatt tatctggctt ataatccgtc ttatttagct     1680

tgttttttca gccggaacag tatttttctc tcacaaaaaa tcagccaaca gtgtttttca     1740

gccggcttat aaacttttgg gatgcaacca tttttgaacc acttttcttt ttttaacgta     1800

ttttctacca cttttcaacc acgcactgac gcacgctatg catgtaattc aacaagtact     1860

cttattactt actttgtcta attgactggt ttaccatcac cctggacctg caggcaaagc     1920

aagatgtgga cactgccgtg tcggtcacgc aggaaatcaa agttctacga cgacatttgt     1980

acggcgcgcg gtacgcatct tagcgtcctc actctcatct tctccggcca gcacagccgc     2040

aatacacgca cacacgtact ctcggaacgg tcactacaca gtctgatgtg ctgcaccgta     2100

ccggcctgca atgcaaccat gcatatcatc gatcatgtgt ctcacagtgc cgtctgtgtc     2160

ctttccctta ggcgatcctg atcttgagct tcacgagctg agtgcccgcc agccatgcat     2220

gcatgatgtc caccagacat gcatgcatgg cacacctagc agctcgccat gcataggact     2280

agctagctat aggacgatga tgatctgagc tccatccagg accatgtgca tgcaacagcg     2340

cgcgacagat gaagatgaca attgctagcc tggtcatcca tcgtccacac aaaaatatct     2400

ttgctacctc aaagcaagga ggaaacctac acagataaca actgactagc ctgcagggga     2460

tgaatcttca tacatactcc agtacatagc tcgctcgctg gtcatttggt caacagcggc     2520

agcatgcgtc gtcaaacaca agctaaatgc cttttacccg tcccgtgtat catcaaaagt     2580

taacaaacct acctgtcagg cagcagcgta tatgtgaaac aagaaatgga tggaagagtc     2640

cgtgagaaag taaaggtgaa agatacgtgc tactgctatc cgttgaatag caataaacac     2700

gggcttagct gttacctacc cgttgatacg gcggaggcca aacgtgtaaa gcagcttatt     2760

ttttttaatg agagagtgta aagcagctac ttagctgggc agacagccca tccacgcgtc     2820

caaagctgct tggctctcgc gcgctataaa tccgacccat ggccacaccc cgtcatccac     2880

atccacacac acaacagaga gaagacgaat tcaatcacta gcatctcgga tccaaaacaa     2940

caagactcaa cacaacacaa caagacacag caaagcaact tatattgcaa cgcatcgtca     3000

gcacattcca aatcgaagtt cacggttcaa gagcaaggtg ccttgatcga actctttgga     3060

gaatttcagc aaacatggcg gctctcgtca tcctgggatt ccttttcctg tcggttgcgg     3120

tgcaaggaaa ggttttcgag cgctgcgagc ttgcccggac gctgaagaaa ctggggctgg     3180

acggttacaa gggtgtttcc cttgctaact ggctgtgcct taccaagtgg gagtcctcct     3240

acaacaccaa ggccacgaac tataacccct cctccgagtc taccgattac gggatcttcc     3300

agattaactc caagtggtgg tgcaacgacg gaaagacccc taacgcggtg gacggttgcc     3360

acgtgtcctg ctccgagctt atggagaacg atattgccaa ggcggttgcg tgcgccaagc     3420

atattgtgtc cgagcagggt atcaccgcgt gggtcgcctg gaagagccac tgcagggacc     3480

acgatgtgag cagctacgtg gagggctgca ccctctgatg tactgagatc ttcattgcag     3540

tttttaaagt attttatata tttactattt cagtgagggt ctccctcctt agtattatat     3600

atgtacttca gaaatagtag tcattctgca ggggagtgag gttcacctcc aaccctatgg     3660

ttactatttc ttactagcgt cgaactacat tacggacacc ctgttgtgtg gttctaccac     3720

gagtcaggag ctgcgagtat tgtagcaaga gaagaattcg gatcccccgg gctgcaggaa     3780

ttcgatatca agcttatcga taccgtcgag gggtccgcaa aaatcaccag tctctctcta     3840

caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga     3900

attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta     3960

tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt     4020

<210>  44
<211>  3993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SPRP-BvLz(m)-3'SrMV UTR-35S

<220>
<221>  promoter
<222>  (1)..(3016)
<223>  SPRP Promoter

<220>
<221>  misc_feature
<222>  (3048)..(3491)
<223>  BvLz (m) coding sequence

<220>
<221>  misc_feature
<222>  (3510)..(3736)
<223>  3' Untranslated Region of SrMV

<220>
<221>  terminator
<222>  (3797)..(3993)
<223>  35S

<400>  44
atatttgcta gagatgttca aagtgatcat ctcagagcaa tatacggaaa gatgtttgaa       60

aaaatacttt cataagtgtt tggtattgac caagcaatga cccgatacat gtatcgccct      120

tagaacaaat atgaccgatg tatacatcgc ccttagttgc tgattagccg atgagttggg      180

tattgttgct tttcagtttt gaattgtgtt tttagattca tgcattattc acttgaaaac      240

agggagcata tattgacact caaattgggc ctctgcctat ttaaataggc ccatgacagt      300

tctggcacaa tctaaggaag actctataaa gcaacatgat aatagtacca tattggtttt      360

cctataggat aagaccaccc ctactcctta taaaataagg ggtatatgac tgattgagtt      420

tccaactatc taatcgataa aaatagatct actacctagt tttacctttt tccaactctc      480

ttgctgtttg ttgcatctct ttgcgagatt catcggcgtt ctaggcggcc ttgctggtcc      540

tagaataaca ctaggcgtgc tcctcgacgg gtcctctcga gaggcattcc caagcttttg      600

cggcgttgaa tgagttcaag atcggcctaa ccgttctcta gatcggttta gtcggtctta      660

gagttgtttg ctacgtgttt aaagttgtgt gcagcctagg gcgagctagc ccttgctgac      720

gtgtgactta gatcacaatc taacgtctaa cagctggctt gactttgcat taaaaaaaaa      780

gagtagtgtg caagtgtgga agcgtcgttt ttttatttga aaaaacaaaa aaatgcgcag      840

tatattaagg gacatcctaa ttaagaggct aagagcaaat gcacaacagt gtactccacg      900

agagattggt cctgtaagtt gtggatgcat gttagcacag gcacaacagg ccagtagttg      960

gggcgtcagt gtcaacgatg tcgggtcggt ggcaggagag ggtgggaaaa catccacgag     1020

cagaaaagga catcgccgtt ggaacaaggg acgagtgcac cactccggcc acgccgtacc     1080

gtacgcctca gaccccgcca cagcgcgttc gctagctgct gctgagcctg atgcctgaac     1140

acggtcgggt cgccaccaca ctgtccatca tctatccgtt cattcatcca cgactgtgct     1200

tgcacagcca tacactgccg aacctagctc gtgtttagat gcaatttttt tttacaaaat     1260

gctcctgtag cactttttta ttgttatttg gaaattagtg tctaatcgtg aactaattag     1320

gcttaaaaga ttcatctcgt gaatttcatc taaactgtgt aattagtttt attttttatc     1380

tatatttaat gcttcatgca tgtgtctaaa gattcgatgt gatgggaaat attgaaaatt     1440

tttgcaaaat tttttgcatc taaactgagc cctacacctc ggtcccagac cgttcgtcga     1500

tgaatctgga caactacctg tccagatttt tattcctata ccaacagttt tttgaatgga     1560

gggagtacga agtcgccagt acgtgtccct tttaacctgt agtgataaaa ctgtgaattt     1620

ctagataaac ttttgggatg aggccctatt tatctggctt ataatccgtc ttatttagct     1680

tgttttttca gccggaacag tatttttctc tcacaaaaaa tcagccaaca gtgtttttca     1740

gccggcttat aaacttttgg gatgcaacca tttttgaacc acttttcttt ttttaacgta     1800

ttttctacca cttttcaacc acgcactgac gcacgctatg catgtaattc aacaagtact     1860

cttattactt actttgtcta attgactggt ttaccatcac cctggacctg caggcaaagc     1920

aagatgtgga cactgccgtg tcggtcacgc aggaaatcaa agttctacga cgacatttgt     1980

acggcgcgcg gtacgcatct tagcgtcctc actctcatct tctccggcca gcacagccgc     2040

aatacacgca cacacgtact ctcggaacgg tcactacaca gtctgatgtg ctgcaccgta     2100

ccggcctgca atgcaaccat gcatatcatc gatcatgtgt ctcacagtgc cgtctgtgtc     2160

ctttccctta ggcgatcctg atcttgagct tcacgagctg agtgcccgcc agccatgcat     2220

gcatgatgtc caccagacat gcatgcatgg cacacctagc agctcgccat gcataggact     2280

agctagctat aggacgatga tgatctgagc tccatccagg accatgtgca tgcaacagcg     2340

cgcgacagat gaagatgaca attgctagcc tggtcatcca tcgtccacac aaaaatatct     2400

ttgctacctc aaagcaagga ggaaacctac acagataaca actgactagc ctgcagggga     2460

tgaatcttca tacatactcc agtacatagc tcgctcgctg gtcatttggt caacagcggc     2520

agcatgcgtc gtcaaacaca agctaaatgc cttttacccg tcccgtgtat catcaaaagt     2580

taacaaacct acctgtcagg cagcagcgta tatgtgaaac aagaaatgga tggaagagtc     2640

cgtgagaaag taaaggtgaa agatacgtgc tactgctatc cgttgaatag caataaacac     2700

gggcttagct gttacctacc cgttgatacg gcggaggcca aacgtgtaaa gcagcttatt     2760

ttttttaatg agagagtgta aagcagctac ttagctgggc agacagccca tccacgcgtc     2820

caaagctgct tggctctcgc gcgctataaa tccgacccat ggccacaccc cgtcatccac     2880

atccacacac acaacagaga ctactcgggc actaccaaca gctgctctag agaaagagag     2940

agagaggcag agagctagca acacacagca gagagagaac tagcaggcga acttgttgga     3000

ggagcagcgg ctagccgaat tcctgcagcc ccatctcgga tccaaacatg gcggccctgg     3060

tgatcctggg cttcctgttc ctgtccgtgg ctgtgcaggg caaggtgttc gaaaggtgcg     3120

aactggctag gaccctgaag aagctgggcc tggatggcta caagggcgtg tccctggcta     3180

actggctgtg cctgaccaag tgggaatcct cctacaacac caaggctacc aactacaacc     3240

catcctccga atccaccgac tacggcatct tccagatcaa ctccaagtgg tggtgcaacg     3300

atggcaagac cccaaacgct gtggatggct gccacgtgtc ctgctccgag ctgatggaaa     3360

acgatatcgc taaggctgtg gcttgcgcta agcacatcgt gtccgaacag ggcatcaccg     3420

cctgggtggc ttggaagtcc cactgcaggg atcacgatgt gtcctcctac gtggaaggct     3480

gcaccctgtg attcgaattc ggatcccccg atcttcattg cagtttttaa agtattttat     3540

atatttacta tttcagtgag ggtctccctc cttagtatta tatatgtact tcagaaatag     3600

tagtcattct gcaggggagt gaggttcacc tccaacccta tggttactat ttcttactag     3660

cgtcgaacta cattacggac accctgttgt gtggttctac cacgagtcag gagctgcgag     3720

tattgtagca agagaagaat tgggctgcag gaattcgata tcaagcttat cgataccgtc     3780

gaggggtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc     3840

agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca     3900

tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa     3960

taaaatttct aattcctaaa accaaaatcc agt                                  3993

<210>  45
<211>  4045
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SPRP-BvLz(m)-35S NOS

<220>
<221>  promoter
<222>  (1)..(3016)
<223>  SPRP Promoter

<220>
<221>  misc_feature
<222>  (3048)..(3491)
<223>  BvLz (m) coding sequence

<220>
<221>  terminator
<222>  (3565)..(3761)
<223>  35SExp

<220>
<221>  terminator
<222>  (3793)..(4045)
<223>  NOS

<400>  45
atatttgcta gagatgttca aagtgatcat ctcagagcaa tatacggaaa gatgtttgaa       60

aaaatacttt cataagtgtt tggtattgac caagcaatga cccgatacat gtatcgccct      120

tagaacaaat atgaccgatg tatacatcgc ccttagttgc tgattagccg atgagttggg      180

tattgttgct tttcagtttt gaattgtgtt tttagattca tgcattattc acttgaaaac      240

agggagcata tattgacact caaattgggc ctctgcctat ttaaataggc ccatgacagt      300

tctggcacaa tctaaggaag actctataaa gcaacatgat aatagtacca tattggtttt      360

cctataggat aagaccaccc ctactcctta taaaataagg ggtatatgac tgattgagtt      420

tccaactatc taatcgataa aaatagatct actacctagt tttacctttt tccaactctc      480

ttgctgtttg ttgcatctct ttgcgagatt catcggcgtt ctaggcggcc ttgctggtcc      540

tagaataaca ctaggcgtgc tcctcgacgg gtcctctcga gaggcattcc caagcttttg      600

cggcgttgaa tgagttcaag atcggcctaa ccgttctcta gatcggttta gtcggtctta      660

gagttgtttg ctacgtgttt aaagttgtgt gcagcctagg gcgagctagc ccttgctgac      720

gtgtgactta gatcacaatc taacgtctaa cagctggctt gactttgcat taaaaaaaaa      780

gagtagtgtg caagtgtgga agcgtcgttt ttttatttga aaaaacaaaa aaatgcgcag      840

tatattaagg gacatcctaa ttaagaggct aagagcaaat gcacaacagt gtactccacg      900

agagattggt cctgtaagtt gtggatgcat gttagcacag gcacaacagg ccagtagttg      960

gggcgtcagt gtcaacgatg tcgggtcggt ggcaggagag ggtgggaaaa catccacgag     1020

cagaaaagga catcgccgtt ggaacaaggg acgagtgcac cactccggcc acgccgtacc     1080

gtacgcctca gaccccgcca cagcgcgttc gctagctgct gctgagcctg atgcctgaac     1140

acggtcgggt cgccaccaca ctgtccatca tctatccgtt cattcatcca cgactgtgct     1200

tgcacagcca tacactgccg aacctagctc gtgtttagat gcaatttttt tttacaaaat     1260

gctcctgtag cactttttta ttgttatttg gaaattagtg tctaatcgtg aactaattag     1320

gcttaaaaga ttcatctcgt gaatttcatc taaactgtgt aattagtttt attttttatc     1380

tatatttaat gcttcatgca tgtgtctaaa gattcgatgt gatgggaaat attgaaaatt     1440

tttgcaaaat tttttgcatc taaactgagc cctacacctc ggtcccagac cgttcgtcga     1500

tgaatctgga caactacctg tccagatttt tattcctata ccaacagttt tttgaatgga     1560

gggagtacga agtcgccagt acgtgtccct tttaacctgt agtgataaaa ctgtgaattt     1620

ctagataaac ttttgggatg aggccctatt tatctggctt ataatccgtc ttatttagct     1680

tgttttttca gccggaacag tatttttctc tcacaaaaaa tcagccaaca gtgtttttca     1740

gccggcttat aaacttttgg gatgcaacca tttttgaacc acttttcttt ttttaacgta     1800

ttttctacca cttttcaacc acgcactgac gcacgctatg catgtaattc aacaagtact     1860

cttattactt actttgtcta attgactggt ttaccatcac cctggacctg caggcaaagc     1920

aagatgtgga cactgccgtg tcggtcacgc aggaaatcaa agttctacga cgacatttgt     1980

acggcgcgcg gtacgcatct tagcgtcctc actctcatct tctccggcca gcacagccgc     2040

aatacacgca cacacgtact ctcggaacgg tcactacaca gtctgatgtg ctgcaccgta     2100

ccggcctgca atgcaaccat gcatatcatc gatcatgtgt ctcacagtgc cgtctgtgtc     2160

ctttccctta ggcgatcctg atcttgagct tcacgagctg agtgcccgcc agccatgcat     2220

gcatgatgtc caccagacat gcatgcatgg cacacctagc agctcgccat gcataggact     2280

agctagctat aggacgatga tgatctgagc tccatccagg accatgtgca tgcaacagcg     2340

cgcgacagat gaagatgaca attgctagcc tggtcatcca tcgtccacac aaaaatatct     2400

ttgctacctc aaagcaagga ggaaacctac acagataaca actgactagc ctgcagggga     2460

tgaatcttca tacatactcc agtacatagc tcgctcgctg gtcatttggt caacagcggc     2520

agcatgcgtc gtcaaacaca agctaaatgc cttttacccg tcccgtgtat catcaaaagt     2580

taacaaacct acctgtcagg cagcagcgta tatgtgaaac aagaaatgga tggaagagtc     2640

cgtgagaaag taaaggtgaa agatacgtgc tactgctatc cgttgaatag caataaacac     2700

gggcttagct gttacctacc cgttgatacg gcggaggcca aacgtgtaaa gcagcttatt     2760

ttttttaatg agagagtgta aagcagctac ttagctgggc agacagccca tccacgcgtc     2820

caaagctgct tggctctcgc gcgctataaa tccgacccat ggccacaccc cgtcatccac     2880

atccacacac acaacagaga ctactcgggc actaccaaca gctgctctag agaaagagag     2940

agagaggcag agagctagca acacacagca gagagagaac tagcaggcga acttgttgga     3000

ggagcagcgg ctagccgaat tcctgcagcc ccatctcgga tccaaacatg gcggccctgg     3060

tgatcctggg cttcctgttc ctgtccgtgg ctgtgcaggg caaggtgttc gaaaggtgcg     3120

aactggctag gaccctgaag aagctgggcc tggatggcta caagggcgtg tccctggcta     3180

actggctgtg cctgaccaag tgggaatcct cctacaacac caaggctacc aactacaacc     3240

catcctccga atccaccgac tacggcatct tccagatcaa ctccaagtgg tggtgcaacg     3300

atggcaagac cccaaacgct gtggatggct gccacgtgtc ctgctccgag ctgatggaaa     3360

acgatatcgc taaggctgtg gcttgcgcta agcacatcgt gtccgaacag ggcatcaccg     3420

cctgggtggc ttggaagtcc cactgcaggg atcacgatgt gtcctcctac gtggaaggct     3480

gcaccctgtg attcgaattc ggatcccccg ggctgcagga attcgatatc aagcttatcg     3540

ataccgtcga ggggtccgca aaaatcacca gtctctctct acaaatctat ctctctctat     3600

ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt     3660

ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact     3720

tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gggccgctcg     3780

acgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt     3840

gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt     3900

aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta     3960

tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc     4020

gcggtgtcat ctatgttact agatc                                           4045

<210>  46
<211>  2936
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SEF1alpha-BvLz(m)-3'SrMV UTR-35S

<220>
<221>  promoter
<222>  (1)..(1959)
<223>  SEF1alpha Promoter

<220>
<221>  misc_feature
<222>  (1991)..(2434)
<223>  BvLz (m) coding sequence

<220>
<221>  misc_feature
<222>  (2453)..(2679)
<223>  3' Untranslated Region of SrMV

<220>
<221>  terminator
<222>  (2740)..(2936)
<223>  35S

<400>  46
ataaactcaa gctttcacca atactttctc cgtttcaaat tataagatgt tttagttttt       60

ttagatacat tatttttact atgtatcaag acattgtata tttttaagtg cgtaccaaaa      120

gtcataaatc taaaaaagtc aaacgtctta taatttaaaa tggagggagg actttctaac      180

tcataccact tgttcccttg ctggttatta attactacat acaaagacca aaattgaata      240

gccaaacttg attctcaaac caactatttt atcaaaatct atgcttttgt ccatttccaa      300

gcaagggaaa ttagttgtga acgtgcaaag tagtaaagga cccctttcca aaagggagac      360

gagcccacat tgttaggaca aaaaaatctt tagtatatta gttctttatt taaagtctat      420

ataattctac tccatatatg acattaaagt gtaactggta tctaaagatc taagagcata      480

acaagcataa aattcaaact tattaaatct aggatccccg tacctcccaa ctctttctgc      540

agtttaattc gctcacaacg cctccttctt tgatgttttt ttccgctgta cctgtgccag      600

tataccaaaa ttttaatttt tttgagcgac caaaatacct tttcgaattt aattttcatg      660

tttcatttta gttttactac gtggtatcca ccatatacta cgtatacaag agcaactcca      720

agagatttgg taaaattaga tgctaaattg tgagatttag ccattatgta aaatagaaag      780

tctatctaaa atgtagaatt ttaaaaccag cctaactaaa ttggaaaaca caaatagcaa      840

gtaggactcg ctagggaaat atggccagcg agaatggtag gatagccagc tagaaaaaat      900

aaaaaccaat atagacagct gttgtaatgt ttttttaaag aacattagct gtaaatcgct      960

ttactaatac attttgctca tggccacgag gcaggggtcg ggtcttgggt cttttttttt     1020

ttgaaacttg gtaaaacttc attactcggc taacaaatcg ttagcaacgg agtctatcca     1080

tataaaaaac aatagtatgt gtaggtcgaa tgctgttttt gttcatttgt ggcccatgaa     1140

gtgttttttt tgggcccaat agcccattca tccatgcctg aaccctaggg cgtcttcctt     1200

ataaaaacct agctccattc tgttctcaaa ccccaacacg cagtcggccg ccgcagaccg     1260

ggagtagccg acgcgccgtc accgtatcct cagatcagcg gcgagccgta accaagcaac     1320

tctgctgacg cccgacgagg tactccgccg cacgcgcgcg cgcttctctt ccttttcttt     1380

tcgctgtgct ttgagcctgt ttgtttgatg actagatcta ctgggtttgt cgtctatgtg     1440

tgatgagacg agccgattca tgcactggat ttctaatcaa gtgttgtttc cgccgctgct     1500

acctctattt agtgtctatg tatgaatttg gttgcagttt acaactgatt tgtcgagcca     1560

taaattataa ccgtttggtg gttctagact agatccagtt tccgatctat gatattacgt     1620

ggctgaggca cttaactctg ttttgtgtgt aagaactgag ccgattcatg tgctggagta     1680

ctaatccaag ttttcctttc gcctcgtcta actctagtat gtactccgta tataaacttg     1740

attgcagttt gcaactgatt ctcggccata gattattact gttgcgttgt tcattagatc     1800

cagtttccga tctctgattt acctgcgtag ggtacttcgt ctttggattt tcctgtcctt     1860

gttgattgtt tgattactgg tttatttcca tatatttatt tctaactgtt tttatctgct     1920

attttgatgt aagcagcagt gtagcgtttc ccttcagccg aattcctgca gccccatctc     1980

ggatccaaac atggcggccc tggtgatcct gggcttcctg ttcctgtccg tggctgtgca     2040

gggcaaggtg ttcgaaaggt gcgaactggc taggaccctg aagaagctgg gcctggatgg     2100

ctacaagggc gtgtccctgg ctaactggct gtgcctgacc aagtgggaat cctcctacaa     2160

caccaaggct accaactaca acccatcctc cgaatccacc gactacggca tcttccagat     2220

caactccaag tggtggtgca acgatggcaa gaccccaaac gctgtggatg gctgccacgt     2280

gtcctgctcc gagctgatgg aaaacgatat cgctaaggct gtggcttgcg ctaagcacat     2340

cgtgtccgaa cagggcatca ccgcctgggt ggcttggaag tcccactgca gggatcacga     2400

tgtgtcctcc tacgtggaag gctgcaccct gtgattcgaa ttcggatccc ccgatcttca     2460

ttgcagtttt taaagtattt tatatattta ctatttcagt gagggtctcc ctccttagta     2520

ttatatatgt acttcagaaa tagtagtcat tctgcagggg agtgaggttc acctccaacc     2580

ctatggttac tatttcttac tagcgtcgaa ctacattacg gacaccctgt tgtgtggttc     2640

taccacgagt caggagctgc gagtattgta gcaagagaag aattgggctg caggaattcg     2700

atatcaagct tatcgatacc gtcgaggggt ccgcaaaaat caccagtctc tctctacaaa     2760

tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta     2820

gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg     2880

tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagt         2936

<210>  47
<211>  2988
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SEF1alpha-BvLz(m)-35S NOS

<220>
<221>  promoter
<222>  (1)..(1959)
<223>  SEF1alpha Promoter

<220>
<221>  misc_feature
<222>  (1991)..(2434)
<223>  BvLz (m) coding sequence

<220>
<221>  terminator
<222>  (2508)..(2704)
<223>  35S

<220>
<221>  terminator
<222>  (2736)..(2988)
<223>  NOS

<400>  47
ataaactcaa gctttcacca atactttctc cgtttcaaat tataagatgt tttagttttt       60

ttagatacat tatttttact atgtatcaag acattgtata tttttaagtg cgtaccaaaa      120

gtcataaatc taaaaaagtc aaacgtctta taatttaaaa tggagggagg actttctaac      180

tcataccact tgttcccttg ctggttatta attactacat acaaagacca aaattgaata      240

gccaaacttg attctcaaac caactatttt atcaaaatct atgcttttgt ccatttccaa      300

gcaagggaaa ttagttgtga acgtgcaaag tagtaaagga cccctttcca aaagggagac      360

gagcccacat tgttaggaca aaaaaatctt tagtatatta gttctttatt taaagtctat      420

ataattctac tccatatatg acattaaagt gtaactggta tctaaagatc taagagcata      480

acaagcataa aattcaaact tattaaatct aggatccccg tacctcccaa ctctttctgc      540

agtttaattc gctcacaacg cctccttctt tgatgttttt ttccgctgta cctgtgccag      600

tataccaaaa ttttaatttt tttgagcgac caaaatacct tttcgaattt aattttcatg      660

tttcatttta gttttactac gtggtatcca ccatatacta cgtatacaag agcaactcca      720

agagatttgg taaaattaga tgctaaattg tgagatttag ccattatgta aaatagaaag      780

tctatctaaa atgtagaatt ttaaaaccag cctaactaaa ttggaaaaca caaatagcaa      840

gtaggactcg ctagggaaat atggccagcg agaatggtag gatagccagc tagaaaaaat      900

aaaaaccaat atagacagct gttgtaatgt ttttttaaag aacattagct gtaaatcgct      960

ttactaatac attttgctca tggccacgag gcaggggtcg ggtcttgggt cttttttttt     1020

ttgaaacttg gtaaaacttc attactcggc taacaaatcg ttagcaacgg agtctatcca     1080

tataaaaaac aatagtatgt gtaggtcgaa tgctgttttt gttcatttgt ggcccatgaa     1140

gtgttttttt tgggcccaat agcccattca tccatgcctg aaccctaggg cgtcttcctt     1200

ataaaaacct agctccattc tgttctcaaa ccccaacacg cagtcggccg ccgcagaccg     1260

ggagtagccg acgcgccgtc accgtatcct cagatcagcg gcgagccgta accaagcaac     1320

tctgctgacg cccgacgagg tactccgccg cacgcgcgcg cgcttctctt ccttttcttt     1380

tcgctgtgct ttgagcctgt ttgtttgatg actagatcta ctgggtttgt cgtctatgtg     1440

tgatgagacg agccgattca tgcactggat ttctaatcaa gtgttgtttc cgccgctgct     1500

acctctattt agtgtctatg tatgaatttg gttgcagttt acaactgatt tgtcgagcca     1560

taaattataa ccgtttggtg gttctagact agatccagtt tccgatctat gatattacgt     1620

ggctgaggca cttaactctg ttttgtgtgt aagaactgag ccgattcatg tgctggagta     1680

ctaatccaag ttttcctttc gcctcgtcta actctagtat gtactccgta tataaacttg     1740

attgcagttt gcaactgatt ctcggccata gattattact gttgcgttgt tcattagatc     1800

cagtttccga tctctgattt acctgcgtag ggtacttcgt ctttggattt tcctgtcctt     1860

gttgattgtt tgattactgg tttatttcca tatatttatt tctaactgtt tttatctgct     1920

attttgatgt aagcagcagt gtagcgtttc ccttcagccg aattcctgca gccccatctc     1980

ggatccaaac atggcggccc tggtgatcct gggcttcctg ttcctgtccg tggctgtgca     2040

gggcaaggtg ttcgaaaggt gcgaactggc taggaccctg aagaagctgg gcctggatgg     2100

ctacaagggc gtgtccctgg ctaactggct gtgcctgacc aagtgggaat cctcctacaa     2160

caccaaggct accaactaca acccatcctc cgaatccacc gactacggca tcttccagat     2220

caactccaag tggtggtgca acgatggcaa gaccccaaac gctgtggatg gctgccacgt     2280

gtcctgctcc gagctgatgg aaaacgatat cgctaaggct gtggcttgcg ctaagcacat     2340

cgtgtccgaa cagggcatca ccgcctgggt ggcttggaag tcccactgca gggatcacga     2400

tgtgtcctcc tacgtggaag gctgcaccct gtgattcgaa ttcggatccc ccgggctgca     2460

ggaattcgat atcaagctta tcgataccgt cgaggggtcc gcaaaaatca ccagtctctc     2520

tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa     2580

gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta     2640

tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc     2700

cagtgacctg caggggccgc tcgacgaatt tccccgatcg ttcaaacatt tggcaataaa     2760

gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga     2820

attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt     2880

ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg     2940

caaactagga taaattatcg cgcgcggtgt catctatgtt actagatc                  2988

<210>  48
<211>  3621
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette JAS-BvLz(m)-35S

<220>
<221>  promoter
<222>  (54)..(2681)
<223>  JAS Promoter

<220>
<221>  misc_feature
<222>  (2692)..(3135)
<223>  BvLz (m) coding sequence

<220>
<221>  terminator
<222>  (3425)..(3621)
<223>  35S

<400>  48
tctagataat acgactcact atagggcacg cgtggtcgac ggcccgggct ggtctgcgac       60

agctagaggc gccaccgcgt cctagcttcc tccaacttct cgtcggagat cccttcaggg      120

atgcccaatg ccaccgcccc taagtcaacc tgcgggagct ggagcttcgc cagggtcaga      180

gctgcggcag caccctggta gaccgcattc ctgatgaccc gcggggtgcg ctccatgaag      240

aagtgcattc gcccaaccaa gtcgagtggg tcgcctggag ggggcgggga agcaaaacgt      300

tgcatgcacc tagcgccctg gcagcgagct cctgtagtat cacctgcgtc gcctccagct      360

catgctcgca agcctccagg gcggcccggc agtgctccaa cactttcgcc tcctcctaca      420

gctccttcca catgcagtcg tgctccgcac gcaccttctc caccttttta ctcttttctt      480

tctcttttct tggcccatct ttggtatttt cacaaatgtc cccctacaaa tgataaatca      540

ccaaaactca tggagcttgc tagttataaa ctctaattct aagtttggtg tttatttgag      600

tggattttgt gtgaaagttg gtggttagaa ataggagtta aggaccgcca acaagatccc      660

ccacacttag ccctttgctc atcctcgagt aaagttcaag gactaaggtg gaacatctcc      720

tcaaatggta cgatgcctgc atataagtta ttccaagcct cacctataca tgtgaacttt      780

gaagtgtcta ccacgccatc ttgggtggtt gagaaatgga acagatcaga atccagtcat      840

ctttacctct cttgcttaga taacttgggt ttttgtaagg ttttcaaatt taaaacatag      900

tcttgctcct caaatgattc tctcatatag ctcaatgtgt atggtttctc accaaggcaa      960

tgttttgcct cttttcatcc tacttctaat atttcttttg tggagcttag ggtagggaat     1020

gaaaaggaag catacttgca ttgcatatgt tactaagtca aaaaccaaat ctgaggagaa     1080

gcaagtcata caatctgatc aagatgtgca agtgtgtgga tatgtggatt aagataactc     1140

ctgtttattc atgctctcct ccttaataaa ctttagaggg catggcaatc tttgcatggg     1200

ccttcatgag ctcatcgtat gtctaagcat ggagctcatc atttatataa gcatggtgat     1260

accaaaatta ctccttttga gcatgtttat atttaggagg acgttttacc tgttgaggta     1320

aatctgaacg ctaataaatc ggctaagcaa aataatttat cacctgttga ttctaacaat     1380

ttgatgatgg acaatattga tgaggtgact gacaaatgat tgaaggcttt aaaggagatt     1440

gagaaggata aatctacaat aaaaatgtaa agaagaaagc attcaaagtg tgagatctgg     1500

tgtggaagac tattttgcct cttgggggta aaagacaaca agtttagtaa gtggcctcaa     1560

aattgggagg gcccatgcaa gattgttaaa gtaattgttt tggattgacg gaggcatttc     1620

aaggtgatca tctacctaga gctctcaatg ggaggtgctc gaagacatat tacccatgtg     1680

tatggcaaga tgtttagcta gtaactgact gatagtgtaa acgatctcca atggggcaag     1740

acatattacc taaggccagg ctggtttttg caagttcgag taggatatag agattctcgt     1800

gcgagttgta aacgatctcc aatggggcaa gacatcctac cctatatata gtgaaggggc     1860

agtagctgat tgagaatcaa tcaatcaagc acaatataat ttattaattt tttattcaaa     1920

cccaattttt ttccttttcc aaccctaatt atagttttcc ttttgcctct aggacaaatt     1980

gacgtgttcc gggtatcctg ctgaattaag aacaacccta ggtgcacctg tcccgataga     2040

gtcccacctg ggtaggcatt catagggatt cgtgtatttc ctgcaaaaaa gcgattaagc     2100

tggcttctaa aactggctag gccggattct gtggccttca ctaccaggtg attttcatgt     2160

gatccgtgca ttctagcact ttgctatgta acccaaactt aagtcgacaa ctataaatat     2220

gctacttgca ggatgttatc acgacacaac tcctaatcta cggaagccta agtttagttt     2280

tgctcggaga caagcaattg tggccagtca ctatagctac gtcagagggt agtgggagca     2340

gttgcgtcgt tggattgaaa acaggtggat cgtatcagat attatgcatt cacatggaca     2400

gtaaatgtgg tacagtaact tcgcaaacaa taaaatctgt cacaatttat tagtgcactc     2460

ctctgacgta aatgcttcta cgtcagagga tttgattccg agggccgctg cacccatcac     2520

taatgacggt ctttacccat catcatggac cattgttcac atccatgcta tcactgtcgt     2580

cctgtccatg cactgcagcc ctctataaat actggcatcc ctcccccgtt cacagatcac     2640

acaacacaag caagaaataa acggtagctg ccataactag tggatccaaa catggcggcc     2700

ctggtgatcc tgggcttcct gttcctgtcc gtggctgtgc agggcaaggt gttcgaaagg     2760

tgcgaactgg ctaggaccct gaagaagctg ggcctggatg gctacaaggg cgtgtccctg     2820

gctaactggc tgtgcctgac caagtgggaa tcctcctaca acaccaaggc taccaactac     2880

aacccatcct ccgaatccac cgactacggc atcttccaga tcaactccaa gtggtggtgc     2940

aacgatggca agaccccaaa cgctgtggat ggctgccacg tgtcctgctc cgagctgatg     3000

gaaaacgata tcgctaaggc tgtggcttgc gctaagcaca tcgtgtccga acagggcatc     3060

accgcctggg tggcttggaa gtcccactgc agggatcacg atgtgtcctc ctacgtggaa     3120

ggctgcaccc tgtgattcga attcggatcc gaattgatct tcattgcagt ttttaaagta     3180

ttttatatat ttactatttc agtgagggtc tccctcctta gtattatata tgtacttcag     3240

aaatagtagt cattctgcag gggagtgagg ttcacctcca accctatggt tactatttct     3300

tactagcgtc gaactacatt acggacaccc tgttgtgtgg ttctaccacg agtcaggagc     3360

tgcgagtatt gtagcaagag aagaattatc aagcttatcg ataccgtcga ggggtccgca     3420

aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt     3480

gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat     3540

ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa     3600

ttcctaaaac caaaatccag t                                               3621

<210>  49
<211>  3855
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette JAS-BvLz(m)-3'SrMV UTR-35S

<220>
<221>  promoter
<222>  (54)..(2681)
<223>  JAS Promoter

<220>
<221>  misc_feature
<222>  (2694)..(3137)
<223>  BvLz (m) coding sequence

<220>
<221>  misc_feature
<222>  (3158)..(3384)
<223>  3' Untranslated Region of SrMV

<220>
<221>  terminator
<222>  (3659)..(3855)
<223>  35S

<400>  49
tctagataat acgactcact atagggcacg cgtggtcgac ggcccgggct ggtctgcgac       60

agctagaggc gccaccgcgt cctagcttcc tccaacttct cgtcggagat cccttcaggg      120

atgcccaatg ccaccgcccc taagtcaacc tgcgggagct ggagcttcgc cagggtcaga      180

gctgcggcag caccctggta gaccgcattc ctgatgaccc gcggggtgcg ctccatgaag      240

aagtgcattc gcccaaccaa gtcgagtggg tcgcctggag ggggcgggga agcaaaacgt      300

tgcatgcacc tagcgccctg gcagcgagct cctgtagtat cacctgcgtc gcctccagct      360

catgctcgca agcctccagg gcggcccggc agtgctccaa cactttcgcc tcctcctaca      420

gctccttcca catgcagtcg tgctccgcac gcaccttctc caccttttta ctcttttctt      480

tctcttttct tggcccatct ttggtatttt cacaaatgtc cccctacaaa tgataaatca      540

ccaaaactca tggagcttgc tagttataaa ctctaattct aagtttggtg tttatttgag      600

tggattttgt gtgaaagttg gtggttagaa ataggagtta aggaccgcca acaagatccc      660

ccacacttag ccctttgctc atcctcgagt aaagttcaag gactaaggtg gaacatctcc      720

tcaaatggta cgatgcctgc atataagtta ttccaagcct cacctataca tgtgaacttt      780

gaagtgtcta ccacgccatc ttgggtggtt gagaaatgga acagatcaga atccagtcat      840

ctttacctct cttgcttaga taacttgggt ttttgtaagg ttttcaaatt taaaacatag      900

tcttgctcct caaatgattc tctcatatag ctcaatgtgt atggtttctc accaaggcaa      960

tgttttgcct cttttcatcc tacttctaat atttcttttg tggagcttag ggtagggaat     1020

gaaaaggaag catacttgca ttgcatatgt tactaagtca aaaaccaaat ctgaggagaa     1080

gcaagtcata caatctgatc aagatgtgca agtgtgtgga tatgtggatt aagataactc     1140

ctgtttattc atgctctcct ccttaataaa ctttagaggg catggcaatc tttgcatggg     1200

ccttcatgag ctcatcgtat gtctaagcat ggagctcatc atttatataa gcatggtgat     1260

accaaaatta ctccttttga gcatgtttat atttaggagg acgttttacc tgttgaggta     1320

aatctgaacg ctaataaatc ggctaagcaa aataatttat cacctgttga ttctaacaat     1380

ttgatgatgg acaatattga tgaggtgact gacaaatgat tgaaggcttt aaaggagatt     1440

gagaaggata aatctacaat aaaaatgtaa agaagaaagc attcaaagtg tgagatctgg     1500

tgtggaagac tattttgcct cttgggggta aaagacaaca agtttagtaa gtggcctcaa     1560

aattgggagg gcccatgcaa gattgttaaa gtaattgttt tggattgacg gaggcatttc     1620

aaggtgatca tctacctaga gctctcaatg ggaggtgctc gaagacatat tacccatgtg     1680

tatggcaaga tgtttagcta gtaactgact gatagtgtaa acgatctcca atggggcaag     1740

acatattacc taaggccagg ctggtttttg caagttcgag taggatatag agattctcgt     1800

gcgagttgta aacgatctcc aatggggcaa gacatcctac cctatatata gtgaaggggc     1860

agtagctgat tgagaatcaa tcaatcaagc acaatataat ttattaattt tttattcaaa     1920

cccaattttt ttccttttcc aaccctaatt atagttttcc ttttgcctct aggacaaatt     1980

gacgtgttcc gggtatcctg ctgaattaag aacaacccta ggtgcacctg tcccgataga     2040

gtcccacctg ggtaggcatt catagggatt cgtgtatttc ctgcaaaaaa gcgattaagc     2100

tggcttctaa aactggctag gccggattct gtggccttca ctaccaggtg attttcatgt     2160

gatccgtgca ttctagcact ttgctatgta acccaaactt aagtcgacaa ctataaatat     2220

gctacttgca ggatgttatc acgacacaac tcctaatcta cggaagccta agtttagttt     2280

tgctcggaga caagcaattg tggccagtca ctatagctac gtcagagggt agtgggagca     2340

gttgcgtcgt tggattgaaa acaggtggat cgtatcagat attatgcatt cacatggaca     2400

gtaaatgtgg tacagtaact tcgcaaacaa taaaatctgt cacaatttat tagtgcactc     2460

ctctgacgta aatgcttcta cgtcagagga tttgattccg agggccgctg cacccatcac     2520

taatgacggt ctttacccat catcatggac cattgttcac atccatgcta tcactgtcgt     2580

cctgtccatg cactgcagcc ctctataaat actggcatcc ctcccccgtt cacagatcac     2640

acaacacaag caagaaataa acggtagctg ccataactag tccggatcca aacatggcgg     2700

ccctggtgat cctgggcttc ctgttcctgt ccgtggctgt gcagggcaag gtgttcgaaa     2760

ggtgcgaact ggctaggacc ctgaagaagc tgggcctgga tggctacaag ggcgtgtccc     2820

tggctaactg gctgtgcctg accaagtggg aatcctccta caacaccaag gctaccaact     2880

acaacccatc ctccgaatcc accgactacg gcatcttcca gatcaactcc aagtggtggt     2940

gcaacgatgg caagacccca aacgctgtgg atggctgcca cgtgtcctgc tccgagctga     3000

tggaaaacga tatcgctaag gctgtggctt gcgctaagca catcgtgtcc gaacagggca     3060

tcaccgcctg ggtggcttgg aagtcccact gcagggatca cgatgtgtcc tcctacgtgg     3120

aaggctgcac cctgtgattc gaaaattcgg atccgatgat cttcattgca gtttttaaag     3180

tattttatat atttactatt tcagtgaggg tctccctcct tagtattata tatgtacttc     3240

agaaatagta gtcattctgc aggggagtga ggttcacctc caaccctatg gttactattt     3300

cttactagcg tcgaactaca ttacggacac cctgttgtgt ggttctacca cgagtcagga     3360

gctgcgagta ttgtagcaag agaagaattg atcttcattg cagtttttaa agtattttat     3420

atatttacta tttcagtgag ggtctccctc cttagtatta tatatgtact tcagaaatag     3480

tagtcattct gcaggggagt gaggttcacc tccaacccta tggttactat ttcttactag     3540

cgtcgaacta cattacggac accctgttgt gtggttctac cacgagtcag gagctgcgag     3600

tattgtagca agagaagaat tatcaagctt atcgataccg tcgaggggtc cgcaaaaatc     3660

accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag     3720

ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga     3780

aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta     3840

aaaccaaaat ccagt                                                      3855

<210>  50
<211>  2854
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette SCBV21-BvLz(m)-35S NOS

<220>
<221>  promoter
<222>  (1)..(1816)
<223>  SCBV21 Promoter

<220>
<221>  misc_feature
<222>  (1857)..(2300)
<223>  BvLz (m) coding sequence

<220>
<221>  terminator
<222>  (2374)..(2570)
<223>  35S

<220>
<221>  terminator
<222>  (2602)..(2854)
<223>  NOS

<400>  50
gaagaacagc atgctgaaca tctgtggaag atgctacaga tatgcaagaa gaatgggtta       60

atcttaagcc cttccaagta taaattggag taaaaagagt tgactttctt ggttcaacaa      120

ttggagataa tcagttagct gttcaagaac atatagtctc caagatagct gattttgatg      180

aagaacgtct caagaccaag gaaggactga aaagctggct ggcaacactc aattatgcca      240

gaaatcacat caaggatatg ggaaaactcc ttggaccctt atatcctaaa acttcagaaa      300

agggagcaaa aggattaaat tctgaagatt ggaaattaat cagcagaatc aagacaatgg      360

tcagaaatct gccaaatctg actattccac cagaggatgc atatattatc attgaaacag      420

atgcttgtgc aactggttgg ggtgcagttt gcaaatggaa gaaatccaag gcagacccaa      480

gaagctccga gctcatatgt cgatatgcaa gtgggaaatt tgacaaacca aaagggacat      540

gtgatgcaga aatctatgga gtaatgaatg ggctggagaa aatgagactc ttttatcttg      600

ataaaaggga aatcactgtg aggacagata gtgccgcaat agagaggttc tacaacaaga      660

gtgttgaaca taaaccctca gaaatccgtt ggataaggtt tatggactat atcactggag      720

caggaccaaa gattgtgatt gagcatatca aaggaaaaca caatggtctg gcagatatcc      780

tctcaagatt gaaagcaaaa ctggcagaat caccttcaga agaagtggtt ttacttgcga      840

aagctttaaa ggaagttgca tactatcctg aacacccgca agtgccaaaa ctaattgaat      900

ggggaaagca aattcttgat ccatttccca agttcaagaa ggacatgttt gaaaaaactg      960

aacacatcat gatggctagt caagagccta cactgctttg tggatgtaga aggcctgcat     1020

atcagttcac atctggcaca aaactcaacc caagcaggaa gttctataaa tgtgcaatga     1080

acatgtgcca ctgctggtat tgggcagatc ttttagaaga atatgtccaa gaacgaattg     1140

aagtgttcat gattgagaac tttgacaaga aaatgggaat tcaagatgta ccaagtacat     1200

caaatgctaa cattccagga aattttaaat ctcttgcaga tttgaagaag gataaagaag     1260

ctaaagctga atatcaagac atgcttgata atcatcgttc aagcattatt gacagaccaa     1320

ggccaacaga tgaacacttc aagcctggat acatgtacac cgattccctg cagaagatca     1380

aggaggacta cgcaagccca agacaggagg aaccaccatg agaagacatt gagttctggt     1440

tatgcaagga agaagactac cacacagaag acctcaatac agaagatgca gttgatctta     1500

ctgacgtaag caatgacgat cagtggaggc gatcgtaagc aatgatgcac ggaaggacaa     1560

ttatggagcg tggaggaccc atcaagcact cagaacgcga acctcaactt tcggcgccag     1620

caccttgtat ctttagttgg tgtgtgtctt tttcggcatc tgtgccacct tacctttgtc     1680

ggccacgttg cctatgctta gcacctacgc aagcatagcg ctcggctggt gtgtgttccc     1740

tctgcctata taaggcatgg ttgtaagact cttacactca tcggtagttc accacatgat     1800

catttgagca agtttgaatc gaattcccgc ggccgccatg catctcggat ccaaacatgg     1860

cggccctggt gatcctgggc ttcctgttcc tgtccgtggc tgtgcagggc aaggtgttcg     1920

aaaggtgcga actggctagg accctgaaga agctgggcct ggatggctac aagggcgtgt     1980

ccctggctaa ctggctgtgc ctgaccaagt gggaatcctc ctacaacacc aaggctacca     2040

actacaaccc atcctccgaa tccaccgact acggcatctt ccagatcaac tccaagtggt     2100

ggtgcaacga tggcaagacc ccaaacgctg tggatggctg ccacgtgtcc tgctccgagc     2160

tgatggaaaa cgatatcgct aaggctgtgg cttgcgctaa gcacatcgtg tccgaacagg     2220

gcatcaccgc ctgggtggct tggaagtccc actgcaggga tcacgatgtg tcctcctacg     2280

tggaaggctg caccctgtga ttcgaattcg gatcccccgg gctgcaggaa ttcgatatca     2340

agcttatcga taccgtcgag gggtccgcaa aaatcaccag tctctctcta caaatctatc     2400

tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc     2460

ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg     2520

taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg     2580

ggccgctcga cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg     2640

aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat     2700

gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc     2760

ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa     2820

ttatcgcgcg cggtgtcatc tatgttacta gatc                                 2854
