                         SEQUENCE LISTING


<110>  DiMarchi, Richard

       Kou, Binbin

       Cheng, Shujiang



<120>  AMIDE-BASED INSULIN PRODRUGS



<130>  32993-216461



<140>  61358192

<141>  2010-06-24



<160>  71


<170>  PatentIn version 3.3



<210>  1
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  1

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  2
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  2

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is threonine, histidine, arginine or lysine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenyalanine, 4-amino 
       phenylalanine or a modified 4-amino phenylalanine (having a 
       dipeptide linked via an amide bond at the aromatic ring amine)

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is asparagine, glycine, alanine glutamine, 
       glutamate, threonine, or serine

<400>  3

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Xaa 
            20      


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  4

Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  5
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine, threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa at position 16 is tyrosine or 4-hydroxymethyl phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa at position 25 is tyrosine, phenylalanine or 4-hydroxymethyl 
       phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is aspartate, a lysine-proline dipeptide, or a
       proline-lysine dipeptide

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is threonine or an arginine-arginine dipeptide

<400>  5

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Xaa 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Xaa Tyr Thr Xaa Xaa 
            20                  25                  


<210>  6
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin a chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is 4-amino phenylalanine

<400>  6

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Asn 
            20      


<210>  7
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  7

His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  8
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  8

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  9
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  9

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  10
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  10

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


<210>  11
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminus of the B chain of an insulin analog

<400>  11

Phe Val Asn Gln 
1               


<210>  12
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glucine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  12

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  13
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  13

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  14
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine, threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is tyrosine, 4-hydroxymethyl phenylalanine,  
       4-amino phenylalanine or a modified 4-amino phenylalanine (having
       a dipeptide linked via an amide bond at the aromatic ring amine)

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is tyrosine, phenylalanine, 4-hydroxymethyl 
       phenylalanine,  4-amino phenyalanine or a modified 4-amino 
       phenylalanine (having a dipeptide linked via an amide bond at the
       aromatic ring amine)

<400>  14

Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Xaa Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Xaa 
            20      


<210>  15
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine, threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa at position 16 is tyrosine, 4-hydroxymethyl phenylalanine,  
       4-amino phenyalanine or a modified 4-amino phenylalanine (having 
       a dipeptide linked via an amide bond at the aromatic amine)

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa at position 25 is tyrosine, phenylalanine, 4-hydroxymethyl 
       phenylalanine,  4-amino phenyalanine or a modified 4-amino 
       phenylalanine (having a dipeptide linked via an amide bond at the
       aromatic ring amine)

<400>  15

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Xaa 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Xaa Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  16
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine, threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa at position 16  is tyrosine, 4-hydroxymethyl phenylalanine,  
       4-amino phenyalanine or a modified 4-amino phenylalanine (having 
       a dipeptide linked via an amide bond at the aromatic ring amine)

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa at position 25 is tyrosine, phenylalanine, 4-hydroxymethyl 
       phenylalanine,  4-amino phenyalanine or a modified 4-amino 
       phenylalanine (having a dipeptide linked via an amide bond at the
       aromatic ring amine)

<400>  16

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Xaa 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Xaa Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is threonine, histidine, arginine or lysine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is asparagine, glycine, alanine glutamine, 
       glutamate, threonine, or serine

<400>  17

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Xaa 
            20      


<210>  18
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenyalanine, 4-amino 
       phenylalanine or a modified 4-amino phenylalanine (having a 
       dipeptide linked via an amide bond at the aromatic ring amine)

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is asparagine, glycine, alanine glutamine, 
       glutamate, threonine, or serine

<400>  18

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Xaa 
            20      


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine, threonine or serine

<400>  19

Xaa Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  20
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine, threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa at position 16 is tyrosine or 4-hydroxymethyl phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa at position 25 is tyrosine, phenylalanine or 4-hydroxymethyl 
       phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is aspartate, a lysine-proline dipeptide, or a
       proline-lysine dipeptide

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is threonine or an arginine-arginine dipeptide

<400>  20

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Xaa 
            20                  25                  


<210>  21
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N-terminus of the B chain of an insulin analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine or desamino-phenylalanine.

<400>  21

Xaa Val Asn Gln 
1               


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  22

Gly Gly Gly Pro Gly Lys Arg 
1               5           


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  23

Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  24

Gly Gly Gly Gly Gly Lys Arg 
1               5           


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  25

Arg Arg Gly Gly Gly Gly Gly 
1               5           


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  26

Gly Gly Ala Pro Gly Asp Val Lys Arg 
1               5                   


<210>  27
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  27

Arg Arg Ala Pro Gly Asp Val Gly Gly 
1               5                   


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  28

Gly Gly Tyr Pro Gly Asp Val Lys Arg 
1               5                   


<210>  29
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  29

Arg Arg Tyr Pro Gly Asp Val Gly Gly 
1               5                   


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  30

Gly Gly His Pro Gly Asp Val Lys Arg 
1               5                   


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  31

Arg Arg His Pro Gly Asp Val Gly Gly 
1               5                   


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  32

Arg Arg Gly Pro Gly Gly Gly 
1               5           


<210>  33
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single chain human insulin analog


<220>
<221>  MISC_FEATURE
<222>  (49)..(49)
<223>  Xaa at position 49 is 4-amino phenyalanine or a modified 4-amino 
       phenylalanine (having a dipeptide linked via an amide bond at the
       aromatic ring amine)

<400>  33

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Gly Ile 
            20                  25                  30          


Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 
        35                  40                  45              


Xaa Cys Asn 
    50      


<210>  34
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single-chain human insulin analog


<220>
<221>  MISC_FEATURE
<222>  (61)..(61)
<223>  Xaa at position 61 is 4-amino phenyalanine or a modified 4-amino 
       phenylalanine (having a dipeptide linked via an amide bond at the
       aromatic ring amine)

<400>  34

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Gln Pro 
            20                  25                  30          


Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys 
        35                  40                  45              


Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Xaa Cys Asn 
    50                  55                  60              


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  35

Ala Gly Arg Gly Ser Gly Lys 
1               5           


<210>  36
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  36

Ala Gly Leu Gly Ser Gly Lys 
1               5           


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  37

Ala Gly Met Gly Ser Gly Lys 
1               5           


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  38

Ala Ser Trp Gly Ser Gly Lys 
1               5           


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  39

Thr Gly Leu Gly Ser Gly Gln 
1               5           


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  40

Thr Gly Leu Gly Arg Gly Lys 
1               5           


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  41

Thr Gly Leu Gly Ser Gly Lys 
1               5           


<210>  42
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  42

His Gly Leu Tyr Ser Gly Lys 
1               5           


<210>  43
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  43

Lys Gly Leu Gly Ser Gly Gln 
1               5           


<210>  44
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  44

Val Gly Leu Met Ser Gly Lys 
1               5           


<210>  45
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  45

Val Gly Leu Ser Ser Gly Gln 
1               5           


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  46

Val Gly Leu Tyr Ser Gly Lys 
1               5           


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  47

Val Gly Leu Ser Ser Gly Lys 
1               5           


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  48

Val Gly Met Ser Ser Gly Lys 
1               5           


<210>  49
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  49

Val Trp Ser Ser Ser Gly Lys 
1               5           


<210>  50
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  50

Val Gly Ser Ser Ser Gly Lys 
1               5           


<210>  51
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  51

Val Gly Met Ser Ser Gly Lys 
1               5           


<210>  52
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  52

Thr Gly Leu Gly Ser Gly Arg 
1               5           


<210>  53
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  53

Thr Gly Leu Gly Lys Gly Gln 
1               5           


<210>  54
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  54

Lys Gly Leu Ser Ser Gly Gln 
1               5           


<210>  55
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  55

Val Lys Leu Ser Ser Gly Gln 
1               5           


<210>  56
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  56

Val Gly Leu Lys Ser Gly Gln 
1               5           


<210>  57
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  57

Thr Gly Leu Gly Lys Gly Gln 
1               5           


<210>  58
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  58

Val Gly Leu Ser Lys Gly Gln 
1               5           


<210>  59
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide for testing cleavage of dipeptide prodrug 
       element

<400>  59

His Ser Arg Gly Thr Phe 
1               5       


<210>  60
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide for testing cleavage of dipeptide prodrug 
       element


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Sarcosine

<400>  60

Lys Xaa His Ser Arg Gly Thr Phe 
1               5               


<210>  61
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  61

Gly Ile Val Asp Glu Cys Cys Phe Arg Ser Cys Asp Leu Arg Arg Leu 
1               5                   10                  15      


Glu Met Tyr Cys Ala 
            20      


<210>  62
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  62

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Gln Phe 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr 
            20                  25                  


<210>  63
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glucagon analogue


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  d-histidine

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  d-threonine

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  d-arginine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  d-threonine

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  d-phenylalanine

<400>  63

Xaa Xaa Xaa Gly Xaa Xaa 
1               5       


<210>  64
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glucagon analogue


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  d-lysine

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  d-sarcosine

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  d-histidine

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  d-threonine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  d-arginine

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  d-threonine

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  d-phenylalanine

<400>  64

Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa 
1               5               


<210>  65
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  65

Ser Arg Val Ser Arg Arg Ser Arg 
1               5               


<210>  66
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  66

Gly Tyr Gly Ser Ser Ser Arg Arg  
1               5                           


<210>  67
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  67

Gly Ser Ser Ser Arg Arg Ala Pro 
1               5               


<210>  68
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF1 B and A chain analogs


<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa at position 22 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (36)..(36)
<223>  Xaa at position 36 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (41)..(41)
<223>  Xaa at position 41 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (42)..(42)
<223>  Xaa at position 42 is ornithine

<400>  68

Cys Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Gly Ile Val Asp Glu 
            20                  25                  30          


Cys Cys Phe Xaa Ser Cys Asp Leu Xaa Xaa Leu Glu Asn Tyr Cys Asn 
        35                  40                  45              


<210>  69
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  The Xaa at position 7 is ornithine

<400>  69

Gly Tyr Gly Ser Ser Ser Xaa Arg 
1               5               


<210>  70
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  70

Gly Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10


<210>  71
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  The Xaa at position 7 is ornithine, arginine or lysine


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  The Xaa at position 8 is ornithine, arginine or lysine


<400>  71

Gly Tyr Gly Ser Ser Ser Xaa Xaa 
1               5                             
