                         SEQUENCE LISTING

<110>  Universit degli studi di Milano
 
<120>  A SEQUENCE SPECIFIC FOR BOIS NOIR PHYTOPLASMA 
       (BN), USES THEREOF AND BN DIAGNOSTIC KITS

<130>  BW570R

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  1122
<212>  DNA
<213>  Phytoplasma sp.


<220>
<221>  allele
<222>  (64)..(64)
<223>  when nucleotide 64 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (66)..(66)
<223>  when nucleotide 66 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (75)..(75)
<223>  when nucleotide 75 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (81)..(81)
<223>  when nucleotide 81 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (105)..(105)
<223>  when nucleotide 105 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (208)..(208)
<223>  when nucleotide 208 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (252)..(253)
<223>  when nucleotide 252 is C and nucleotide 253 is A the sequence is 
       specific for the phytoplasmas sp. associated with vine Bois Noir 
       (Legno Nero)

<220>
<221>  allele
<222>  (264)..(264)
<223>  when nucleotide 264 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (288)..(288)
<223>  when nucleotide 288 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (368)..(368)
<223>  when nucleotide 368 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (370)..(370)
<223>  when nucleotide 370 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (372)..(372)
<223>  when nucleotide 372 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (412)..(412)
<223>  when nucleotide 412 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (445)..(445)
<223>  when nucleotide 445 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (481)..(481)
<223>  when nucleotide 481 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (493)..(493)
<223>  when nucleotide 493 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (497)..(497)
<223>  when nucleotide 497 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (506)..(506)
<223>  when nucleotide 506 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (512)..(512)
<223>  when nucleotide 512 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (544)..(544)
<223>  when nucleotide 544 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (547)..(547)
<223>  when nucleotide 547 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (565)..(565)
<223>  when nucleotide 565 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (571)..(571)
<223>  when nucleotide 571 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (574)..(574)
<223>  when nucleotide 574 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (589)..(589)
<223>  when nucleotide 589 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (610)..(610)
<223>  when nucleotide 610 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (616)..(616)
<223>  when nucleotide 616 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (649)..(649)
<223>  when nucleotide 649 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (652)..(652)
<223>  when nucleotide 652 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (663)..(663)
<223>  when nucleotide 663 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (671)..(673)
<223>  when nucleotide 671 is G, nucleotide 672 is G, nucleotide 673 is 
       C the sequence is specific for the phytoplasmas sp. associated 
       with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (685)..(685)
<223>  when nucleotide 685 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (695)..(695)
<223>  when nucleotide 695 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (708)..(708)
<223>  when nucleotide 708 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (712)..(712)
<223>  when nucleotide 712 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (721)..(721)
<223>  when nucleotide 721 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (733)..(733)
<223>  when nucleotide 733 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (736)..(736)
<223>  when nucleotide 736 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (745)..(745)
<223>  when nucleotide 745 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (804)..(804)
<223>  when nucleotide 804 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (815)..(815)
<223>  when nucleotide 815 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (829)..(829)
<223>  when nucleotide 829 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (863)..(863)
<223>  when nucleotide 863 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (874)..(874)
<223>  when nucleotide 874 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (898)..(898)
<223>  when nucleotide 888 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (937)..(937)
<223>  when nucleotide 937is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (946)..(946)
<223>  when nucleotide 946 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (952)..(952)
<223>  when nucleotide 952 is A the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (976)..(976)
<223>  when nucleotide 976 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (979)..(979)
<223>  when nucleotide 979 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (985)..(985)
<223>  when nucleotide 985 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (1010)..(1010)
<223>  when nucleotide 1010 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (1012)..(1012)
<223>  when nucleotide 1012 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (1034)..(1034)
<223>  when nucleotide 1034 is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (1036)..(1036)
<223>  when nucleotide 1036 is T the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (1051)..(1051)
<223>  when nucleotide 1051 is G the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<220>
<221>  allele
<222>  (1064)..(1065)
<223>  when nucleotide 1064 is G, nucleotide 1065 is C the sequence is 
       specific for the phytoplasmas sp. associated with vine Bois Noir 
       (Legno Nero)

<220>
<221>  allele
<222>  (1081)..(1081)
<223>  when nucleotide 1081  is C the sequence is specific for the 
       phytoplasmas sp. associated with vine Bois Noir (Legno Nero)

<400>  1
atgactatgg aaactaaaaa cgctaaagcg attgttcgta aagtttcaat cgcccctcga     60

aaatcccgtt tagtgattga cttaattaga ggaaaaaata tcgatcaagc tcaagccatt    120

ttaactttta ctcctaaagt agctgctccc gttattttaa aacttttaaa tagtgctgtt    180

gcaaatgctt taaataattt aaaattacaa cgtgaacaac tttttgtcaa agaagttttt    240

gtcaacgaag gcatacgttt aaagcgtatg tttccgagag ctaaagggtc tggtgatatg    300

attaaaaaaa gaactagtca catcacttta gtagtggctg ctaaaaactt gcaaacatca    360

aaggaggcga acagtgggtc aaaaaactaa tcctaatggt ttaagattag gtattattag    420

aacttgggat tctcaatggt gtgtcaatga caaagaaata ccagctttaa ttaaagaaga    480

atttttaatt cgcaaaataa ttaatcaatt tggtaaaaaa agcgctatta gtcaaattga    540

aatacagcgt ttaaaagaaa aaaccaaaaa tcggatcaca atttcaattc ataccgccaa    600

accaggaatg attatcggta aagagggtga aactcgcaat aaaatagtag cgcaattaaa    660

aacattaact ggcaaagaca ttaacttaaa tattatagaa gttaaaatcc cagataaaat    720

agctttatta gtagcacaaa atattgctga acaattagaa aaccgaatga aatttcgtcg    780

tgttcaaaaa atggcgatcc aaagagccct taaatctggc gctaaaggga ttaaaatttt    840

aatttctggt cgtcttaatg gcaatgaaat agcgcgtagc gaaggaaatg ctgaaggtcg    900

tgttcctttg cacactttaa gagcagatat tgattatgct gcctacgaag cacacaccac    960

ttatggtgtt ttagggatca aagtctggat ttttcacggt gaagttttat ccggtcaaac   1020

catcctagac actcgtaaac cttttgtttc gcaaaataaa tttgccaaac accctcgtca   1080

ctttaaagga ggtaaataat tatgttaatg ccaaaaagaa ca                      1122


<210>  2
<211>  21
<212>  DNA
<213>  Phytoplasma sp.

<400>  2
tcaattcata ccgccaaacc a                                               21


<210>  3
<211>  18
<212>  DNA
<213>  Phytoplasma sp.

<400>  3
atgattatcg gtaaagag                                                   18


<210>  4
<211>  24
<212>  DNA
<213>  Phytoplasma sp.

<400>  4
tgcgctacta ttttattgcg agtt                                            24


<210>  5
<211>  390
<212>  DNA
<213>  Phytoplasma sp.


<220>
<221>  CDS
<222>  (1)..(390)

<400>  5
atg act atg gaa act aaa aac gct aaa gcg att gtt cgt aaa gtt tca       48
Met Thr Met Glu Thr Lys Asn Ala Lys Ala Ile Val Arg Lys Val Ser         
1               5                   10                  15              

atc gcc cct cga aaa tcc cgt tta gtg att gac tta att aga gga aaa       96
Ile Ala Pro Arg Lys Ser Arg Leu Val Ile Asp Leu Ile Arg Gly Lys         
            20                  25                  30                  

aat atc gat caa gct caa gcc att tta act ttt act cct aaa gta gct      144
Asn Ile Asp Gln Ala Gln Ala Ile Leu Thr Phe Thr Pro Lys Val Ala         
        35                  40                  45                      

gct ccc gtt att tta aaa ctt tta aat agt gct gtt gca aat gct tta      192
Ala Pro Val Ile Leu Lys Leu Leu Asn Ser Ala Val Ala Asn Ala Leu         
    50                  55                  60                          

aat aat tta aaa tta caa cgt gaa caa ctt ttt gtc aaa gaa gtt ttt      240
Asn Asn Leu Lys Leu Gln Arg Glu Gln Leu Phe Val Lys Glu Val Phe         
65                  70                  75                  80          

gtc aac gaa ggc ata cgt tta aag cgt atg ttt ccg aga gct aaa ggg      288
Val Asn Glu Gly Ile Arg Leu Lys Arg Met Phe Pro Arg Ala Lys Gly         
                85                  90                  95              

tct ggt gat atg att aaa aaa aga act agt cac atc act tta gta gtg      336
Ser Gly Asp Met Ile Lys Lys Arg Thr Ser His Ile Thr Leu Val Val         
            100                 105                 110                 

gct gct aaa aac ttg caa aca tca aag gag gcg aac agt ggg tca aaa      384
Ala Ala Lys Asn Leu Gln Thr Ser Lys Glu Ala Asn Ser Gly Ser Lys         
        115                 120                 125                     

aac taa                                                              390
Asn                                                                     
                                                                        


<210>  6
<211>  129
<212>  PRT
<213>  Phytoplasma sp.

<400>  6

Met Thr Met Glu Thr Lys Asn Ala Lys Ala Ile Val Arg Lys Val Ser 
1               5                   10                  15      


Ile Ala Pro Arg Lys Ser Arg Leu Val Ile Asp Leu Ile Arg Gly Lys 
            20                  25                  30          


Asn Ile Asp Gln Ala Gln Ala Ile Leu Thr Phe Thr Pro Lys Val Ala 
        35                  40                  45              


Ala Pro Val Ile Leu Lys Leu Leu Asn Ser Ala Val Ala Asn Ala Leu 
    50                  55                  60                  


Asn Asn Leu Lys Leu Gln Arg Glu Gln Leu Phe Val Lys Glu Val Phe 
65                  70                  75                  80  


Val Asn Glu Gly Ile Arg Leu Lys Arg Met Phe Pro Arg Ala Lys Gly 
                85                  90                  95      


Ser Gly Asp Met Ile Lys Lys Arg Thr Ser His Ile Thr Leu Val Val 
            100                 105                 110         


Ala Ala Lys Asn Leu Gln Thr Ser Lys Glu Ala Asn Ser Gly Ser Lys 
        115                 120                 125             


Asn 
    


<210>  7
<211>  726
<212>  DNA
<213>  Phytoplasma sp.


<220>
<221>  CDS
<222>  (1)..(726)

<400>  7
gtg ggt caa aaa act aat cct aat ggt tta aga tta ggt att att aga       48
Val Gly Gln Lys Thr Asn Pro Asn Gly Leu Arg Leu Gly Ile Ile Arg         
1               5                   10                  15              

act tgg gat tct caa tgg tgt gtc aat gac aaa gaa ata cca gct tta       96
Thr Trp Asp Ser Gln Trp Cys Val Asn Asp Lys Glu Ile Pro Ala Leu         
            20                  25                  30                  

att aaa gaa gaa ttt tta att cgc aaa ata att aat caa ttt ggt aaa      144
Ile Lys Glu Glu Phe Leu Ile Arg Lys Ile Ile Asn Gln Phe Gly Lys         
        35                  40                  45                      

aaa agc gct att agt caa att gaa ata cag cgt tta aaa gaa aaa acc      192
Lys Ser Ala Ile Ser Gln Ile Glu Ile Gln Arg Leu Lys Glu Lys Thr         
    50                  55                  60                          

aaa aat cgg atc aca att tca att cat acc gcc aaa cca gga atg att      240
Lys Asn Arg Ile Thr Ile Ser Ile His Thr Ala Lys Pro Gly Met Ile         
65                  70                  75                  80          

atc ggt aaa gag ggt gaa act cgc aat aaa ata gta gcg caa tta aaa      288
Ile Gly Lys Glu Gly Glu Thr Arg Asn Lys Ile Val Ala Gln Leu Lys         
                85                  90                  95              

aca tta act ggc aaa gac att aac tta aat att ata gaa gtt aaa atc      336
Thr Leu Thr Gly Lys Asp Ile Asn Leu Asn Ile Ile Glu Val Lys Ile         
            100                 105                 110                 

cca gat aaa ata gct tta tta gta gca caa aat att gct gaa caa tta      384
Pro Asp Lys Ile Ala Leu Leu Val Ala Gln Asn Ile Ala Glu Gln Leu         
        115                 120                 125                     

gaa aac cga atg aaa ttt cgt cgt gtt caa aaa atg gcg atc caa aga      432
Glu Asn Arg Met Lys Phe Arg Arg Val Gln Lys Met Ala Ile Gln Arg         
    130                 135                 140                         

gcc ctt aaa tct ggc gct aaa ggg att aaa att tta att tct ggt cgt      480
Ala Leu Lys Ser Gly Ala Lys Gly Ile Lys Ile Leu Ile Ser Gly Arg         
145                 150                 155                 160         

ctt aat ggc aat gaa ata gcg cgt agc gaa gga aat gct gaa ggt cgt      528
Leu Asn Gly Asn Glu Ile Ala Arg Ser Glu Gly Asn Ala Glu Gly Arg         
                165                 170                 175             

gtt cct ttg cac act tta aga gca gat att gat tat gct gcc tac gaa      576
Val Pro Leu His Thr Leu Arg Ala Asp Ile Asp Tyr Ala Ala Tyr Glu         
            180                 185                 190                 

gca cac acc act tat ggt gtt tta ggg atc aaa gtc tgg att ttt cac      624
Ala His Thr Thr Tyr Gly Val Leu Gly Ile Lys Val Trp Ile Phe His         
        195                 200                 205                     

ggt gaa gtt tta tcc ggt caa acc atc cta gac act cgt aaa cct ttt      672
Gly Glu Val Leu Ser Gly Gln Thr Ile Leu Asp Thr Arg Lys Pro Phe         
    210                 215                 220                         

gtt tcg caa aat aaa ttt gcc aaa cac cct cgt cac ttt aaa gga ggt      720
Val Ser Gln Asn Lys Phe Ala Lys His Pro Arg His Phe Lys Gly Gly         
225                 230                 235                 240         

aaa taa                                                              726
Lys                                                                     
                                                                        


<210>  8
<211>  241
<212>  PRT
<213>  Phytoplasma sp.

<400>  8

Val Gly Gln Lys Thr Asn Pro Asn Gly Leu Arg Leu Gly Ile Ile Arg 
1               5                   10                  15      


Thr Trp Asp Ser Gln Trp Cys Val Asn Asp Lys Glu Ile Pro Ala Leu 
            20                  25                  30          


Ile Lys Glu Glu Phe Leu Ile Arg Lys Ile Ile Asn Gln Phe Gly Lys 
        35                  40                  45              


Lys Ser Ala Ile Ser Gln Ile Glu Ile Gln Arg Leu Lys Glu Lys Thr 
    50                  55                  60                  


Lys Asn Arg Ile Thr Ile Ser Ile His Thr Ala Lys Pro Gly Met Ile 
65                  70                  75                  80  


Ile Gly Lys Glu Gly Glu Thr Arg Asn Lys Ile Val Ala Gln Leu Lys 
                85                  90                  95      


Thr Leu Thr Gly Lys Asp Ile Asn Leu Asn Ile Ile Glu Val Lys Ile 
            100                 105                 110         


Pro Asp Lys Ile Ala Leu Leu Val Ala Gln Asn Ile Ala Glu Gln Leu 
        115                 120                 125             


Glu Asn Arg Met Lys Phe Arg Arg Val Gln Lys Met Ala Ile Gln Arg 
    130                 135                 140                 


Ala Leu Lys Ser Gly Ala Lys Gly Ile Lys Ile Leu Ile Ser Gly Arg 
145                 150                 155                 160 


Leu Asn Gly Asn Glu Ile Ala Arg Ser Glu Gly Asn Ala Glu Gly Arg 
                165                 170                 175     


Val Pro Leu His Thr Leu Arg Ala Asp Ile Asp Tyr Ala Ala Tyr Glu 
            180                 185                 190         


Ala His Thr Thr Tyr Gly Val Leu Gly Ile Lys Val Trp Ile Phe His 
        195                 200                 205             


Gly Glu Val Leu Ser Gly Gln Thr Ile Leu Asp Thr Arg Lys Pro Phe 
    210                 215                 220                 


Val Ser Gln Asn Lys Phe Ala Lys His Pro Arg His Phe Lys Gly Gly 
225                 230                 235                 240 


Lys 
    


<210>  9
<211>  1077
<212>  DNA
<213>  Phytoplasma sp.

<400>  9
ggaaattaaa aatgctaaag caattgttcg caaagttcca attgctcctc gaaaagttcg     60

tttagttatt gatttaatta ggggtaaaaa aatcgaccaa gcgcaagcaa ttttaacttt    120

tactcctaac gcttctgcac ttattgtttt aaaactttta aatagtgctg ttgctaatgc    180

tttaaataat ttaaaattaa aacgtgaaca actttacgtc aaagaagttt ttgttaacga    240

aggattaaga ttaaaacgaa tgtttccaag agctaaaggt tctggcgata tgatcaaaaa    300

aagaactagc cacatcactt tagtagtgca aacattaaag gaggaggaaa gtgggtcaaa    360

aaactaatcc taatggctta agattaggaa ttattcgaac ttgggattcc caatggtgcg    420

ttaacgacaa agaaatacca gctttaatta aagaagattt tttaattcgt caattaatta    480

ataattttag caaaagaaac tctattagtc aaattgaaat tcaaagatta aaagaaaaaa    540

ctaaaaaccg cattacaatt acgatccaca ctgccaaacc aggaattatt attggcaaag    600

atggtgaaac tcgtaataaa attcaggccc aattaaaaaa attaactcaa aaagacatta    660

atttaaacat tttggaagtt aaaaatcctg acaaaaccgc tgtattagtg gctcaaaaca    720

tggccgaaca attagaaaat cgtatgaaat tccgtcgcgt tcaaaaaatg gccatccaaa    780

aagcttttaa agcaggcgct aaaggaatta aagtgttgat ttctggtcgt cttaatggcg    840

atgaaatagc acgaagcgaa agacatgatg aaggacgtgt tccgttacat actttgagag    900

cagatattga ttacgccgct ttagaagctc acaccactta cggagtttta ggaattaaag    960

tttggatttt tcatggagaa gttttaccag gtcaaactat tttagacact agaaaacctt   1020

ttgtttctca aaataaattt attaaacgtc ctcgttattt taaaggaggt aaaaata      1077


<210>  10
<211>  1114
<212>  DNA
<213>  Phytoplasma sp.


<220>
<221>  misc_feature
<222>  (558)..(558)
<223>  n is a, c, g, or t

<400>  10
aataactatg gaaaccaaaa acgccaaagc gattgctaga aaagtttcaa tcgcccctcg     60

aaaagcacgt ttagttgttg atttaattcg aggaaaaaat attgcacaag ctcaagccat    120

tttaactttt acccctaaag tagctgctcc cgttatttta aaacttttaa acagtgctgt    180

ttccaatgct gttaataatt taaaattaaa ccgcgaacaa ctttatgtta aagaagtttt    240

tgtcaacgaa ggtttgcgtt taaaacgtat gtttccaaga gctaaaggtt ctggtgatat    300

gattaaaaaa agaaccagcc acattacttt agtaataact tctagcacaa acttgcaaac    360

atcaaaggag gaagaacaaa gtgggtcaaa aaactaatcc taacggctta agattaggca    420

ttattagaac ttgggaatct caatggtgtg ttaatgataa agaaattcct aatttaatta    480

aagaagattt tttaattcgt aaactaatca ataattttac taaaaaaagt gctatcagtc    540

aaattgacat tgaacgcnta aaagaaaaaa ataaaaaccg tatcactatt tctgtccaca    600

ccgctaaacc aggcgttatt attggaaaag atggcgatac acgcaacaaa ttagttgcca    660

aactcaaaga acttacccaa aaagacgtta atcttaacgt gttagaagtt aaaaactctg    720

ataaaatcgc tttattaatt gctcaaaata tggctgaaca actagaaaat cgtatgtttt    780

tccgccgtgt tcaaaaaatg gcaatccaaa aagccctaaa agctggtgcc aaaggagtaa    840

aaactttaat ttctggtcgt ttgggtggtg ctgaaatagc tcgtagcgaa ggacatgccg    900

aaggcagagt tcctctacac actctaagag cagacatcga ttacgctgct gttgaagctc    960

acactactta tggagtttta ggaattaaag tatggatttt ccacggtgaa gttttaccag   1020

gacaaaccat tctagacact agaaaaccgt ttgcttccca atcttctaac actcctaaca   1080

gacgccctcg caatttcaaa ggaggcaaca ataa                               1114


<210>  11
<211>  1170
<212>  DNA
<213>  Phytoplasma sp

<400>  11
tttaaagaag gtatttatat gaaagttaaa gctgtaacaa gtccaatacc tattactcct     60

agaaaagcac gtttagttgc tgatttaata cgtggtaagc atgttaaaga agcagaagct    120

attttaatgt ttacttctaa atcgtctgct cctgttattt ttaaattatt aaaaagtgct    180

gttgcaaatg ctgttcataa ttttaatttt aataaagatg atttatttgt taaagaaata    240

tttgtcgatg aaggtttgcg tttacctcgt ttatttccga gagctaaagg taaaacagat    300

aaaagaaaaa aaagaatgag tcgtgtaaaa atatttcttt cttcatttaa aaaagaaatt    360

caggagatgc aagtaaatgg gtcaaaagag taatcctaat ggtttgagat taggaataat    420

taggacttgg gaatctaaat ggtatgatgt tgataaaaaa gttccttttt tagtcggtga    480

agattttaaa attagaactt taattaaaaa tcattatcct aaatcaacta tttctcaaat    540

agaaatcaaa cgtttaaaaa aatcaaatga tgaatttatt gaaatagatt tatatacttc    600

aaaaataggt atcattcaag gtccggaaaa taagaataaa aatagtttaa ttaataaaat    660

agaaaaatta attaataaaa aagttcaaat taatattttc gaagtaaaag caattaataa    720

aattgctgtt ttagttgctc aaaatatcgc tatgcaattg caacaaagag ctttttataa    780

agctgtttta aaatcagcta ttcaaaaagc tttaaaaagc ggtattaaag gtattaagat    840

tattattaca ggtcgtttag gtggagctga aaaagctaga agagattcta tttctatggg    900

agttgttcct ttgaatactt taagagctga tattgattac gcttttgaag aagcccatac    960

tacttatggt gttttaggtg ttaaagtaat tattaatcat ggtgaggttt tacctaataa   1020

aaccatcgca gatactagac aaatattttc ttctcaatat gaaaataaaa aaaataataa   1080

taaaagacat tttgttgata agaaaaattt taaaaaaagc acgtcttaat ataatcaaaa   1140

gaggggtaca taatatatta tgttaatgcc                                    1170


<210>  12
<211>  1122
<212>  DNA
<213>  phytoplasma sp


<220>
<221>  misc_feature
<222>  (64)..(64)
<223>  when nucleotide 64 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  when nucleotide 66 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (75)..(75)
<223>  when nucleotide 75 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  when nucleotide 81 is  C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (105)..(105)
<223>  when nucleotide 105 is  T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (208)..(208)
<223>  when nucleotide 208 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (252)..(253)
<223>  when nucleotide 252 is C and nucleotide 253 is A the sequence is 
       specific for the phytoplasma sp associated with vine Bois Noir 
       (Legno Nero)

<220>
<221>  misc_feature
<222>  (264)..(264)
<223>  when nucleotide 264 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (288)..(288)
<223>  when nucleotide 288 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (368)..(368)
<223>  when nucleotide 368 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (370)..(370)
<223>  when nucleotide 370 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (372)..(372)
<223>  when nucleotide 372 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (412)..(412)
<223>  when nucleotide 412 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (445)..(445)
<223>  when nucleotide 445 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (481)..(481)
<223>  when nucleotide 481 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (493)..(493)
<223>  when nucleotide 493 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (497)..(497)
<223>  when nucleotide 497 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (506)..(506)
<223>  when nucleotide 506 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (512)..(512)
<223>  when nucleotide 512 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (544)..(544)
<223>  when nucleotide 544 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (547)..(547)
<223>  when nucleotide 547 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (565)..(565)
<223>  when nucleotide 565 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (571)..(571)
<223>  when nucleotide 571 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (574)..(574)
<223>  when nucleotide 574 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (589)..(589)
<223>  when nucleotide 589 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (610)..(610)
<223>  when nucleotide 610 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (616)..(616)
<223>  when nucleotide 616 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (649)..(649)
<223>  when nucleotide 649 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (652)..(652)
<223>  when nucleotide 652 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (663)..(663)
<223>  when nucleotide 663 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (671)..(673)
<223>  when nucleotide 671 is G, nucleotide 672 is G, nucleotide 673 is 
       C the sequence is specific for the phytoplasma sp associated with
       vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (685)..(685)
<223>  when nucleotide 685 is  C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (695)..(695)
<223>  when nucleotide 685 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (708)..(708)
<223>  when nucleotide 708 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (712)..(712)
<223>  when nucleotide 712 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (721)..(721)
<223>  when nucleotide 721 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (733)..(733)
<223>  when nucleotide 733 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (736)..(736)
<223>  when nucleotide 736 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (745)..(745)
<223>  when nucleotide 745 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (804)..(804)
<223>  when nucleotide 804 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (815)..(815)
<223>  when nucleotide 815 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (829)..(829)
<223>  when nucleotide 829 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (863)..(863)
<223>  when nucleotide 863 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (874)..(874)
<223>  when nucleotide 874 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (888)..(888)
<223>  when nucleotide 888 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (898)..(898)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (937)..(937)
<223>  when nucleotide 937 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (943)..(943)
<223>  when nucleotide 943 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (946)..(946)
<223>  when nucleotide 946 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (952)..(952)
<223>  when nucleotide 952 is A the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (976)..(976)
<223>  when nucleotide 976 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (979)..(979)
<223>  when nucleotide 979 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (985)..(985)
<223>  when nucleotide 985 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (1010)..(1010)
<223>  when nucleotide 1010 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (1012)..(1012)
<223>  when nucleotide 1012 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (1034)..(1034)
<223>  when nucleotide 1034 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (1036)..(1036)
<223>  when nucleotide 1036 is T the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (1051)..(1051)
<223>  when nucleotide 1051 is G the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<220>
<221>  misc_feature
<222>  (1064)..(1065)
<223>  when nucleotide 1064 is G, nucleotide 1065is C the sequence is 
       specific for the phytoplasma sp associated with vine Bois Noir 
       (Legno Nero)

<220>
<221>  misc_feature
<222>  (1081)..(1081)
<223>  when nucleotide 1081 is C the sequence is specific for the 
       phytoplasma sp associated with vine Bois Noir (Legno Nero)

<400>  12
atgactatgg aaactaaaaa cgctaaagcg attgttcgta aagtttcaat cgcccctcga     60

aaancccgtt tagtnattga nttaattaga ggaaaaaata tcgancaagc tcaagccatt    120

ttaactttta ctcctaaagt agctgctccc gttattttaa aacttttaaa tagtgctgtt    180

gcaaatgctt taaataattt aaaattanaa cgtgaacaac tttttgtcaa agaagttttt    240

gtcaacgaag gnntacgttt aaancgtatg tttccgagag ctaaaggntc tggtgatatg    300

attaaaaaaa gaactagtca catcacttta gtagtggctg ctaaaaactt gcaaacatca    360

aaggaggngn acagtgggtc aaaaaactaa tcctaatggt ttaagattag gnattattag    420

aacttgggat tctcaatggt gtgtnaatga caaagaaata ccagctttaa ttaaagaaga    480

ntttttaatt cgcaaantaa ttaatnaatt tggtaaaaaa agcgctatta gtcaaattga    540

aatncagcgt ttaaaagaaa aaacnaaaaa ncgnatcaca atttcaatnc ataccgccaa    600

accaggaatn attatnggta aagagggtga aactcgcaat aaaatagtng cncaattaaa    660

aanattaact nnnaaagaca ttaanttaaa tattntagaa gttaaaancc cngataaaat    720

ngctttatta gtngcncaaa atatngctga acaattagaa aaccgaatga aatttcgtcg    780

tgttcaaaaa atggcgatcc aaanagccct taaanctggc gctaaaggna ttaaaatttt    840

aatttctggt cgtcttaatg gcnatgaaat agcncgtagc gaaggaaatg ctgaaggncg    900

tgttcctttg cacactttaa gagcagatat tgattangct gcctangaag cncacaccac    960

ttatggtgtt ttaggnatna aagtntggat ttttcacggt gaagttttan cnggtcaaac   1020

catcctagac actngnaaac cttttgtttc ncaaaataaa tttnncaaac accctcgtca   1080

ntttaaagga ggtaaataat tatgttaatg ccaaaaagaa ca                      1122


