                         SEQUENCE LISTING

<110>  GE, Wayne
       NG, Yue Ling
 
<120>  QUANTITATIVE REAL-TIME PCR ASSAY USING FRET DUAL-LABELED PRIMERS

<130>  LT00288

<140>  Not Yet Assigned
<141>  2011-08-19

<150>  US 61/375318
<151>  2010-08-20

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  54
<212>  DNA
<213>  Equine herpesvirus 1

<400>  1
atctggccgg gcttcaacca tccgtcaact actcgacgtt cgaggtgggt gacc             54


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  2
actcgacgtt cgaggtgggt                                                   20


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  3
tgcaagctcc acccactgg                                                    19


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Quencher molecule attached

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  FAM fluorophore attached

<400>  4
ggtcacccac ctcgaacgt                                                    19


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Quencher molecule attached

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  FAM fluorophore attached

<400>  5
ggtcacccac ctcgaacgt                                                    19


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher molecule attached

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  FAM fluorophore attached

<400>  6
ggtcacccac ctcgaacgt                                                    19


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  7
atctggccgg gcttcaac                                                     18


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Iowa Black FQ quencher attached

<220>
<221>  misc_feature
<222>  (2)..(27)
<223>  Internal fluorophore molecule attached

<400>  8
tgatgcagtg caagctccac ccactgg                                           27


<210>  9
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe sequence

<400>  9
atccgtcaac tactc                                                        15


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  FAM fluorophore attached

<400>  10
tggtcccgtc ctcatctcag                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  FAM fluorophore attached

<400>  11
tggtcccgtc ctcatctcag                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  FAM fluorophore attached

<400>  12
tggtcccgtc ctcatctcag                                                   20


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  FAM fluorophore attached

<400>  13
tggtcccgtc tcatctcag                                                    19


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  14
ccagttcaga atcggacgtt                                                   20


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  15
gctgccttgc cggaaatta                                                    19


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  FAM fluorophore attached

<400>  16
aaagacgcct accctgttga                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  FAM fluorophore attached

<400>  17
aaagacgcct accctgttga                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  FAM fluorophore attached

<400>  18
aaagacgcct accctgttga                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  BHQ1 quencher attached

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  FAM fluorophore attached

<400>  19
aaagacgcct accctgttga                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  20
tggtcccgtc ctcatctcag                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  21
ccagttcaga atcggacgtt                                                   20


<210>  22
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  22
gctgccttgc cggaaatta                                                    19


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  23
aaagacgcct accctgttga                                                   20


<210>  24
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM fluorophore attached

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  NFQ quencher attached

<400>  24
agccatgaag aatgccgtct atcacaca                                          28


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM fluorophore attached

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  NFQ quencher attached

<400>  25
tccatagttc ctggtttgta atgtgccg                                          28


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM fluorophore attached

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  BHQ1 quencher attached

<400>  26
cgctcgttac tcgtcaggca ctcggtgagc g                                      31


<210>  27
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  27
ccaccctggc gctcg                                                        15


<210>  28
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  28
gccgggcttc aaccatcc                                                     18


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  29
gcttcaacca tccgtcaact actcgac                                           27


<210>  30
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  30
aaccatccgt caactactcg acgttc                                            26


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  31
tccgtcaact actcgacgtt cgag                                              24


<210>  32
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  32
caactactcg acgttcgagg tg                                                22


<210>  33
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  33
gccagtgaat tattaatacg actcactata gggagaaga                              39


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence

<400>  34
tcgcgcgttt cggtgatgac                                                   20


<210>  35
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM fluorophore attached

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  MGB fluorophore attached

<400>  35
atccgtcgac tactcg                                                       16


