                         SEQUENCE LISTING

<110>  Universitaet des Saarlandes
 
<120>  Means and methods applying single nucleotide primer extension 
       with ion pair-, reversed-phase HPLC (SIRPH) for the diagnosis of 
       SNPs

<130>  SAR13695PCT


<150>  EP 10187539.1
<151>  2010-10-14

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  5312
<212>  DNA
<213>  Homo Sapiens


<220>
<221>  CDS
<222>  (182)..(748)

<400>  1
ggccgcggcg gcggaggcag cagcggcggc ggcagtggcg gcggcgaagg tggcggcggc       60

tcggccagta ctcccggccc ccgccatttc ggactgggag cgagcgcggc gcaggcactg      120

aaggcggcgg cggggccaga ggctcagcgg ctcccaggtg cgggagagag gcctgctgaa      180

a atg act gaa tat aaa ctt gtg gta gtt gga gct ggt ggc gta ggc aag      229
  Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys         
  1               5                   10                  15              

agt gcc ttg acg ata cag cta att cag aat cat ttt gtg gac gaa tat        277
Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr           
            20                  25                  30                    

gat cca aca ata gag gat tcc tac agg aag caa gta gta att gat gga        325
Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly           
        35                  40                  45                        

gaa acc tgt ctc ttg gat att ctc gac aca gca ggt caa gag gag tac        373
Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr           
    50                  55                  60                            

agt gca atg agg gac cag tac atg agg act ggg gag ggc ttt ctt tgt        421
Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys           
65                  70                  75                  80            

gta ttt gcc ata aat aat act aaa tca ttt gaa gat att cac cat tat        469
Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His Tyr           
                85                  90                  95                

aga gaa caa att aaa aga gtt aag gac tct gaa gat gta cct atg gtc        517
Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met Val           
            100                 105                 110                   

cta gta gga aat aaa tgt gat ttg cct tct aga aca gta gac aca aaa        565
Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr Lys           
        115                 120                 125                       

cag gct cag gac tta gca aga agt tat gga att cct ttt att gaa aca        613
Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr           
    130                 135                 140                           

tca gca aag aca aga cag ggt gtt gat gat gcc ttc tat aca tta gtt        661
Ser Ala Lys Thr Arg Gln Gly Val Asp Asp Ala Phe Tyr Thr Leu Val           
145                 150                 155                 160           

cga gaa att cga aaa cat aaa gaa aag atg agc aaa gat ggt aaa aag        709
Arg Glu Ile Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys           
                165                 170                 175               

aag aaa aag aag tca aag aca aag tgt gta att atg taa atacaatttg         758
Lys Lys Lys Lys Ser Lys Thr Lys Cys Val Ile Met                           
            180                 185                                       

tacttttttc ttaaggcata ctagtacaag tggtaatttt tgtacattac actaaattat      818

tagcatttgt tttagcatta cctaattttt ttcctgctcc atgcagactg ttagctttta      878

ccttaaatgc ttattttaaa atgacagtgg aagttttttt ttcctctaag tgccagtatt      938

cccagagttt tggtttttga actagcaatg cctgtgaaaa agaaactgaa tacctaagat      998

ttctgtcttg gggtttttgg tgcatgcagt tgattacttc ttatttttct taccaattgt     1058

gaatgttggt gtgaaacaaa ttaatgaagc ttttgaatca tccctattct gtgttttatc     1118

tagtcacata aatggattaa ttactaattt cagttgagac cttctaattg gtttttactg     1178

aaacattgag ggaacacaaa tttatgggct tcctgatgat gattcttcta ggcatcatgt     1238

cctatagttt gtcatccctg atgaatgtaa agttacactg ttcacaaagg ttttgtctcc     1298

tttccactgc tattagtcat ggtcactctc cccaaaatat tatatttttt ctataaaaag     1358

aaaaaaatgg aaaaaaatta caaggcaatg gaaactatta taaggccatt tccttttcac     1418

attagataaa ttactataaa gactcctaat agcttttcct gttaaggcag acccagtatg     1478

aaatggggat tattatagca accattttgg ggctatattt acatgctact aaatttttat     1538

aataattgaa aagattttaa caagtataaa aaattctcat aggaattaaa tgtagtctcc     1598

ctgtgtcaga ctgctctttc atagtataac tttaaatctt ttcttcaact tgagtctttg     1658

aagatagttt taattctgct tgtgacatta aaagattatt tgggccagtt atagcttatt     1718

aggtgttgaa gagaccaagg ttgcaaggcc aggccctgtg tgaacctttg agctttcata     1778

gagagtttca cagcatggac tgtgtcccca cggtcatcca gtgttgtcat gcattggtta     1838

gtcaaaatgg ggagggacta gggcagtttg gatagctcaa caagatacaa tctcactctg     1898

tggtggtcct gctgacaaat caagagcatt gcttttgttt cttaagaaaa caaactcttt     1958

tttaaaaatt acttttaaat attaactcaa aagttgagat tttggggtgg tggtgtgcca     2018

agacattaat ttttttttta aacaatgaag tgaaaaagtt ttacaatctc taggtttggc     2078

tagttctctt aacactggtt aaattaacat tgcataaaca cttttcaagt ctgatccata     2138

tttaataatg ctttaaaata aaaataaaaa caatcctttt gataaattta aaatgttact     2198

tattttaaaa taaatgaagt gagatggcat ggtgaggtga aagtatcact ggactaggaa     2258

gaaggtgact taggttctag ataggtgtct tttaggactc tgattttgag gacatcactt     2318

actatccatt tcttcatgtt aaaagaagtc atctcaaact cttagttttt tttttttaca     2378

actatgtaat ttatattcca tttacataag gatacactta tttgtcaagc tcagcacaat     2438

ctgtaaattt ttaacctatg ttacaccatc ttcagtgcca gtcttgggca aaattgtgca     2498

agaggtgaag tttatatttg aatatccatt ctcgttttag gactcttctt ccatattagt     2558

gtcatcttgc ctccctacct tccacatgcc ccatgacttg atgcagtttt aatacttgta     2618

attcccctaa ccataagatt tactgctgct gtggatatct ccatgaagtt ttcccactga     2678

gtcacatcag aaatgcccta catcttattt cctcagggct caagagaatc tgacagatac     2738

cataaaggga tttgacctaa tcactaattt tcaggtggtg gctgatgctt tgaacatctc     2798

tttgctgccc aatccattag cgacagtagg atttttcaaa cctggtatga atagacagaa     2858

ccctatccag tggaaggaga atttaataaa gatagtgctg aaagaattcc ttaggtaatc     2918

tataactagg actactcctg gtaacagtaa tacattccat tgttttagta accagaaatc     2978

ttcatgcaat gaaaaatact ttaattcatg aagcttactt tttttttttg gtgtcagagt     3038

ctcgctcttg tcacccaggc tggaatgcag tggcgccatc tcagctcact gcaacctcca     3098

tctcccaggt tcaagcgatt ctcgtgcctc ggcctcctga gtagctggga ttacaggcgt     3158

gtgccactac actcaactaa tttttgtatt tttaggagag acggggtttc accctgttgg     3218

ccaggctggt ctcgaactcc tgacctcaag tgattcaccc accttggcct cataaacctg     3278

ttttgcagaa ctcatttatt cagcaaatat ttattgagtg cctaccagat gccagtcacc     3338

gcacaaggca ctgggtatat ggtatcccca aacaagagac ataatcccgg tccttaggta     3398

gtgctagtgt ggtctgtaat atcttactaa ggcctttggt atacgaccca gagataacac     3458

gatgcgtatt ttagttttgc aaagaagggg tttggtctct gtgccagctc tataattgtt     3518

ttgctacgat tccactgaaa ctcttcgatc aagctacttt atgtaaatca cttcattgtt     3578

ttaaaggaat aaacttgatt atattgtttt tttatttggc ataactgtga ttcttttagg     3638

acaattactg tacacattaa ggtgtatgtc agatattcat attgacccaa atgtgtaata     3698

ttccagtttt ctctgcataa gtaattaaaa tatacttaaa aattaatagt tttatctggg     3758

tacaaataaa caggtgcctg aactagttca cagacaagga aacttctatg taaaaatcac     3818

tatgatttct gaattgctat gtgaaactac agatctttgg aacactgttt aggtagggtg     3878

ttaagactta cacagtacct cgtttctaca cagagaaaga aatggccata cttcaggaac     3938

tgcagtgctt atgaggggat atttaggcct cttgaatttt tgatgtagat gggcattttt     3998

ttaaggtagt ggttaattac ctttatgtga actttgaatg gtttaacaaa agatttgttt     4058

ttgtagagat tttaaagggg gagaattcta gaaataaatg ttacctaatt attacagcct     4118

taaagacaaa aatccttgtt gaagtttttt taaaaaaagc taaattacat agacttaggc     4178

attaacatgt ttgtggaaga atatagcaga cgtatattgt atcatttgag tgaatgttcc     4238

caagtaggca ttctaggctc tatttaactg agtcacactg cataggaatt tagaacctaa     4298

cttttatagg ttatcaaaac tgttgtcacc attgcacaat tttgtcctaa tatatacata     4358

gaaactttgt ggggcatgtt aagttacagt ttgcacaagt tcatctcatt tgtattccat     4418

tgattttttt tttcttctaa acattttttc ttcaaacagt atataacttt ttttagggga     4478

ttttttttta gacagcaaaa actatctgaa gatttccatt tgtcaaaaag taatgatttc     4538

ttgataattg tgtagtaatg ttttttagaa cccagcagtt accttaaagc tgaatttata     4598

tttagtaact tctgtgttaa tactggatag catgaattct gcattgagaa actgaatagc     4658

tgtcataaaa tgaaactttc tttctaaaga aagatactca catgagttct tgaagaatag     4718

tcataactag attaagatct gtgttttagt ttaatagttt gaagtgcctg tttgggataa     4778

tgataggtaa tttagatgaa tttaggggaa aaaaaagtta tctgcagata tgttgagggc     4838

ccatctctcc ccccacaccc ccacagagct aactgggtta cagtgtttta tccgaaagtt     4898

tccaattcca ctgtcttgtg ttttcatgtt gaaaatactt ttgcattttt cctttgagtg     4958

ccaatttctt actagtacta tttcttaatg taacatgttt acctggaatg tattttaact     5018

atttttgtat agtgtaaact gaaacatgca cattttgtac attgtgcttt cttttgtggg     5078

acatatgcag tgtgatccag ttgttttcca tcatttggtt gcgctgacct aggaatgttg     5138

gtcatatcaa acattaaaaa tgaccactct tttaattgaa attaactttt aaatgtttat     5198

aggagtatgt gctgtgaagt gatctaaaat ttgtaatatt tttgtcatga actgtactac     5258

tcctaattat tgtaatgtaa taaaaatagt tacagtgaca aaaaaaaaaa aaaa           5312


<210>  2
<211>  188
<212>  PRT
<213>  Homo Sapiens

<400>  2

Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 
            20                  25                  30          


Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 
        35                  40                  45              


Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 
    50                  55                  60                  


Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 
65                  70                  75                  80  


Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His Tyr 
                85                  90                  95      


Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met Val 
            100                 105                 110         


Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr Lys 
        115                 120                 125             


Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr 
    130                 135                 140                 


Ser Ala Lys Thr Arg Gln Gly Val Asp Asp Ala Phe Tyr Thr Leu Val 
145                 150                 155                 160 


Arg Glu Ile Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 
                165                 170                 175     


Lys Lys Lys Lys Ser Lys Thr Lys Cys Val Ile Met 
            180                 185             


<210>  3
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the human KRAS gene

<400>  3
ggcctgctga aaatgactga ata                                               23


<210>  4
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the human KRAS gene

<400>  4
ctcatgaaaa tggtcagaga aacct                                             25


<210>  5
<211>  11
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the human KRAS gene

<400>  5
tagttggagc t                                                            11


<210>  6
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the human KRAS gene

<400>  6
gcactcttgc ctacgc                                                       16


<210>  7
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the human KRAS gene

<400>  7
gttggagctg                                                              10


<210>  8
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the human KRAS gene

<400>  8
actcttgcct acg                                                          13


<210>  9
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  N-terminal fragment of the human KRAS gene


<220>
<221>  CDS
<222>  (1)..(60)

<400>  9
atg act gaa tat aaa ctt gtg gta gtt gga gct ggt ggc gta ggc aag         48
Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys           
1               5                   10                  15                

agt gcc ttg acg                                                         60
Ser Ala Leu Thr                                                           
            20                                                            


<210>  10
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  10

Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr 
            20  

<210>  11
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the KRAS gene

<400>  11
aaggcctgct gaaaatgact g                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the KRAS gene

<400>  12
agaatggtcc tgcaccagta a                                                 21


<210>  13
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the BRAF gene

<400>  13
tcataatgct tgctctgata gga                                               23


<210>  14
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the BRAF gene

<400>  14
ggccaaaaat ttaatcagtg g                                                 21


<210>  15
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  primer derived from the BRAF gene

<400>  15
actccatcga gatttc                                                       16


<210>  16
<211>  2301
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(2298)

<400>  16
atg gcg gcg ctg agc ggt ggc ggt ggt ggc ggc gcg gag ccg ggc cag         48
Met Ala Ala Leu Ser Gly Gly Gly Gly Gly Gly Ala Glu Pro Gly Gln           
1               5                   10                  15                

gct ctg ttc aac ggg gac atg gag ccc gag gcc ggc gcc ggc gcc ggc         96
Ala Leu Phe Asn Gly Asp Met Glu Pro Glu Ala Gly Ala Gly Ala Gly           
            20                  25                  30                    

gcc gcg gcc tct tcg gct gcg gac cct gcc att ccg gag gag gtg tgg        144
Ala Ala Ala Ser Ser Ala Ala Asp Pro Ala Ile Pro Glu Glu Val Trp           
        35                  40                  45                        

aat atc aaa caa atg att aag ttg aca cag gaa cat ata gag gcc cta        192
Asn Ile Lys Gln Met Ile Lys Leu Thr Gln Glu His Ile Glu Ala Leu           
    50                  55                  60                            

ttg gac aaa ttt ggt ggg gag cat aat cca cca tca ata tat ctg gag        240
Leu Asp Lys Phe Gly Gly Glu His Asn Pro Pro Ser Ile Tyr Leu Glu           
65                  70                  75                  80            

gcc tat gaa gaa tac acc agc aag cta gat gca ctc caa caa aga gaa        288
Ala Tyr Glu Glu Tyr Thr Ser Lys Leu Asp Ala Leu Gln Gln Arg Glu           
                85                  90                  95                

caa cag tta ttg gaa tct ctg ggg aac gga act gat ttt tct gtt tct        336
Gln Gln Leu Leu Glu Ser Leu Gly Asn Gly Thr Asp Phe Ser Val Ser           
            100                 105                 110                   

agc tct gca tca atg gat acc gtt aca tct tct tcc tct tct agc ctt        384
Ser Ser Ala Ser Met Asp Thr Val Thr Ser Ser Ser Ser Ser Ser Leu           
        115                 120                 125                       

tca gtg cta cct tca tct ctt tca gtt ttt caa aat ccc aca gat gtg        432
Ser Val Leu Pro Ser Ser Leu Ser Val Phe Gln Asn Pro Thr Asp Val           
    130                 135                 140                           

gca cgg agc aac ccc aag tca cca caa aaa cct atc gtt aga gtc ttc        480
Ala Arg Ser Asn Pro Lys Ser Pro Gln Lys Pro Ile Val Arg Val Phe           
145                 150                 155                 160           

ctg ccc aac aaa cag agg aca gtg gta cct gca agg tgt gga gtt aca        528
Leu Pro Asn Lys Gln Arg Thr Val Val Pro Ala Arg Cys Gly Val Thr           
                165                 170                 175               

gtc cga gac agt cta aag aaa gca ctg atg atg aga ggt cta atc cca        576
Val Arg Asp Ser Leu Lys Lys Ala Leu Met Met Arg Gly Leu Ile Pro           
            180                 185                 190                   

gag tgc tgt gct gtt tac aga att cag gat gga gag aag aaa cca att        624
Glu Cys Cys Ala Val Tyr Arg Ile Gln Asp Gly Glu Lys Lys Pro Ile           
        195                 200                 205                       

ggt tgg gac act gat att tcc tgg ctt act gga gaa gaa ttg cat gtg        672
Gly Trp Asp Thr Asp Ile Ser Trp Leu Thr Gly Glu Glu Leu His Val           
    210                 215                 220                           

gaa gtg ttg gag aat gtt cca ctt aca aca cac aac ttt gta cga aaa        720
Glu Val Leu Glu Asn Val Pro Leu Thr Thr His Asn Phe Val Arg Lys           
225                 230                 235                 240           

acg ttt ttc acc tta gca ttt tgt gac ttt tgt cga aag ctg ctt ttc        768
Thr Phe Phe Thr Leu Ala Phe Cys Asp Phe Cys Arg Lys Leu Leu Phe           
                245                 250                 255               

cag ggt ttc cgc tgt caa aca tgt ggt tat aaa ttt cac cag cgt tgt        816
Gln Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Gln Arg Cys           
            260                 265                 270                   

agt aca gaa gtt cca ctg atg tgt gtt aat tat gac caa ctt gat ttg        864
Ser Thr Glu Val Pro Leu Met Cys Val Asn Tyr Asp Gln Leu Asp Leu           
        275                 280                 285                       

ctg ttt gtc tcc aag ttc ttt gaa cac cac cca ata cca cag gaa gag        912
Leu Phe Val Ser Lys Phe Phe Glu His His Pro Ile Pro Gln Glu Glu           
    290                 295                 300                           

gcg tcc tta gca gag act gcc cta aca tct gga tca tcc cct tcc gca        960
Ala Ser Leu Ala Glu Thr Ala Leu Thr Ser Gly Ser Ser Pro Ser Ala           
305                 310                 315                 320           

ccc gcc tcg gac tct att ggg ccc caa att ctc acc agt ccg tct cct       1008
Pro Ala Ser Asp Ser Ile Gly Pro Gln Ile Leu Thr Ser Pro Ser Pro           
                325                 330                 335               

tca aaa tcc att cca att cca cag ccc ttc cga cca gca gat gaa gat       1056
Ser Lys Ser Ile Pro Ile Pro Gln Pro Phe Arg Pro Ala Asp Glu Asp           
            340                 345                 350                   

cat cga aat caa ttt ggg caa cga gac cga tcc tca tca gct ccc aat       1104
His Arg Asn Gln Phe Gly Gln Arg Asp Arg Ser Ser Ser Ala Pro Asn           
        355                 360                 365                       

gtg cat ata aac aca ata gaa cct gtc aat att gat gac ttg att aga       1152
Val His Ile Asn Thr Ile Glu Pro Val Asn Ile Asp Asp Leu Ile Arg           
    370                 375                 380                           

gac caa gga ttt cgt ggt gat gga gga tca acc aca ggt ttg tct gct       1200
Asp Gln Gly Phe Arg Gly Asp Gly Gly Ser Thr Thr Gly Leu Ser Ala           
385                 390                 395                 400           

acc ccc cct gcc tca tta cct ggc tca cta act aac gtg aaa gcc tta       1248
Thr Pro Pro Ala Ser Leu Pro Gly Ser Leu Thr Asn Val Lys Ala Leu           
                405                 410                 415               

cag aaa tct cca gga cct cag cga gaa agg aag tca tct tca tcc tca       1296
Gln Lys Ser Pro Gly Pro Gln Arg Glu Arg Lys Ser Ser Ser Ser Ser           
            420                 425                 430                   

gaa gac agg aat cga atg aaa aca ctt ggt aga cgg gac tcg agt gat       1344
Glu Asp Arg Asn Arg Met Lys Thr Leu Gly Arg Arg Asp Ser Ser Asp           
        435                 440                 445                       

gat tgg gag att cct gat ggg cag att aca gtg gga caa aga att gga       1392
Asp Trp Glu Ile Pro Asp Gly Gln Ile Thr Val Gly Gln Arg Ile Gly           
    450                 455                 460                           

tct gga tca ttt gga aca gtc tac aag gga aag tgg cat ggt gat gtg       1440
Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp Val           
465                 470                 475                 480           

gca gtg aaa atg ttg aat gtg aca gca cct aca cct cag cag tta caa       1488
Ala Val Lys Met Leu Asn Val Thr Ala Pro Thr Pro Gln Gln Leu Gln           
                485                 490                 495               

gcc ttc aaa aat gaa gta gga gta ctc agg aaa aca cga cat gtg aat       1536
Ala Phe Lys Asn Glu Val Gly Val Leu Arg Lys Thr Arg His Val Asn           
            500                 505                 510                   

atc cta ctc ttc atg ggc tat tcc aca aag cca caa ctg gct att gtt       1584
Ile Leu Leu Phe Met Gly Tyr Ser Thr Lys Pro Gln Leu Ala Ile Val           
        515                 520                 525                       

acc cag tgg tgt gag ggc tcc agc ttg tat cac cat ctc cat atc att       1632
Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr His His Leu His Ile Ile           
    530                 535                 540                           

gag acc aaa ttt gag atg atc aaa ctt ata gat att gca cga cag act       1680
Glu Thr Lys Phe Glu Met Ile Lys Leu Ile Asp Ile Ala Arg Gln Thr           
545                 550                 555                 560           

gca cag ggc atg gat tac tta cac gcc aag tca atc atc cac aga gac       1728
Ala Gln Gly Met Asp Tyr Leu His Ala Lys Ser Ile Ile His Arg Asp           
                565                 570                 575               

ctc aag agt aat aat ata ttt ctt cat gaa gac ctc aca gta aaa ata       1776
Leu Lys Ser Asn Asn Ile Phe Leu His Glu Asp Leu Thr Val Lys Ile           
            580                 585                 590                   

ggt gat ttt ggt cta gct aca gtg aaa tct cga tgg agt ggg tcc cat       1824
Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser His           
        595                 600                 605                       

cag ttt gaa cag ttg tct gga tcc att ttg tgg atg gca cca gaa gtc       1872
Gln Phe Glu Gln Leu Ser Gly Ser Ile Leu Trp Met Ala Pro Glu Val           
    610                 615                 620                           

atc aga atg caa gat aaa aat cca tac agc ttt cag tca gat gta tat       1920
Ile Arg Met Gln Asp Lys Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr           
625                 630                 635                 640           

gca ttt gga att gtt ctg tat gaa ttg atg act gga cag tta cct tat       1968
Ala Phe Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Gln Leu Pro Tyr           
                645                 650                 655               

tca aac atc aac aac agg gac cag ata att ttt atg gtg gga cga gga       2016
Ser Asn Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly           
            660                 665                 670                   

tac ctg tct cca gat ctc agt aag gta cgg agt aac tgt cca aaa gcc       2064
Tyr Leu Ser Pro Asp Leu Ser Lys Val Arg Ser Asn Cys Pro Lys Ala           
        675                 680                 685                       

atg aag aga tta atg gca gag tgc ctc aaa aag aaa aga gat gag aga       2112
Met Lys Arg Leu Met Ala Glu Cys Leu Lys Lys Lys Arg Asp Glu Arg           
    690                 695                 700                           

cca ctc ttt ccc caa att ctc gcc tct att gag ctg ctg gcc cgc tca       2160
Pro Leu Phe Pro Gln Ile Leu Ala Ser Ile Glu Leu Leu Ala Arg Ser           
705                 710                 715                 720           

ttg cca aaa att cac cgc agt gca tca gaa ccc tcc ttg aat cgg gct       2208
Leu Pro Lys Ile His Arg Ser Ala Ser Glu Pro Ser Leu Asn Arg Ala           
                725                 730                 735               

ggt ttc caa aca gag gat ttt agt cta tat gct tgt gct tct cca aaa       2256
Gly Phe Gln Thr Glu Asp Phe Ser Leu Tyr Ala Cys Ala Ser Pro Lys           
            740                 745                 750                   

aca ccc atc cag gca ggg gga tat ggt gcg ttt cct gtc cac tga           2301
Thr Pro Ile Gln Ala Gly Gly Tyr Gly Ala Phe Pro Val His                   
        755                 760                 765                       


<210>  17
<211>  766
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Ala Ala Leu Ser Gly Gly Gly Gly Gly Gly Ala Glu Pro Gly Gln 
1               5                   10                  15      


Ala Leu Phe Asn Gly Asp Met Glu Pro Glu Ala Gly Ala Gly Ala Gly 
            20                  25                  30          


Ala Ala Ala Ser Ser Ala Ala Asp Pro Ala Ile Pro Glu Glu Val Trp 
        35                  40                  45              


Asn Ile Lys Gln Met Ile Lys Leu Thr Gln Glu His Ile Glu Ala Leu 
    50                  55                  60                  


Leu Asp Lys Phe Gly Gly Glu His Asn Pro Pro Ser Ile Tyr Leu Glu 
65                  70                  75                  80  


Ala Tyr Glu Glu Tyr Thr Ser Lys Leu Asp Ala Leu Gln Gln Arg Glu 
                85                  90                  95      


Gln Gln Leu Leu Glu Ser Leu Gly Asn Gly Thr Asp Phe Ser Val Ser 
            100                 105                 110         


Ser Ser Ala Ser Met Asp Thr Val Thr Ser Ser Ser Ser Ser Ser Leu 
        115                 120                 125             


Ser Val Leu Pro Ser Ser Leu Ser Val Phe Gln Asn Pro Thr Asp Val 
    130                 135                 140                 


Ala Arg Ser Asn Pro Lys Ser Pro Gln Lys Pro Ile Val Arg Val Phe 
145                 150                 155                 160 


Leu Pro Asn Lys Gln Arg Thr Val Val Pro Ala Arg Cys Gly Val Thr 
                165                 170                 175     


Val Arg Asp Ser Leu Lys Lys Ala Leu Met Met Arg Gly Leu Ile Pro 
            180                 185                 190         


Glu Cys Cys Ala Val Tyr Arg Ile Gln Asp Gly Glu Lys Lys Pro Ile 
        195                 200                 205             


Gly Trp Asp Thr Asp Ile Ser Trp Leu Thr Gly Glu Glu Leu His Val 
    210                 215                 220                 


Glu Val Leu Glu Asn Val Pro Leu Thr Thr His Asn Phe Val Arg Lys 
225                 230                 235                 240 


Thr Phe Phe Thr Leu Ala Phe Cys Asp Phe Cys Arg Lys Leu Leu Phe 
                245                 250                 255     


Gln Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Gln Arg Cys 
            260                 265                 270         


Ser Thr Glu Val Pro Leu Met Cys Val Asn Tyr Asp Gln Leu Asp Leu 
        275                 280                 285             


Leu Phe Val Ser Lys Phe Phe Glu His His Pro Ile Pro Gln Glu Glu 
    290                 295                 300                 


Ala Ser Leu Ala Glu Thr Ala Leu Thr Ser Gly Ser Ser Pro Ser Ala 
305                 310                 315                 320 


Pro Ala Ser Asp Ser Ile Gly Pro Gln Ile Leu Thr Ser Pro Ser Pro 
                325                 330                 335     


Ser Lys Ser Ile Pro Ile Pro Gln Pro Phe Arg Pro Ala Asp Glu Asp 
            340                 345                 350         


His Arg Asn Gln Phe Gly Gln Arg Asp Arg Ser Ser Ser Ala Pro Asn 
        355                 360                 365             


Val His Ile Asn Thr Ile Glu Pro Val Asn Ile Asp Asp Leu Ile Arg 
    370                 375                 380                 


Asp Gln Gly Phe Arg Gly Asp Gly Gly Ser Thr Thr Gly Leu Ser Ala 
385                 390                 395                 400 


Thr Pro Pro Ala Ser Leu Pro Gly Ser Leu Thr Asn Val Lys Ala Leu 
                405                 410                 415     


Gln Lys Ser Pro Gly Pro Gln Arg Glu Arg Lys Ser Ser Ser Ser Ser 
            420                 425                 430         


Glu Asp Arg Asn Arg Met Lys Thr Leu Gly Arg Arg Asp Ser Ser Asp 
        435                 440                 445             


Asp Trp Glu Ile Pro Asp Gly Gln Ile Thr Val Gly Gln Arg Ile Gly 
    450                 455                 460                 


Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp Val 
465                 470                 475                 480 


Ala Val Lys Met Leu Asn Val Thr Ala Pro Thr Pro Gln Gln Leu Gln 
                485                 490                 495     


Ala Phe Lys Asn Glu Val Gly Val Leu Arg Lys Thr Arg His Val Asn 
            500                 505                 510         


Ile Leu Leu Phe Met Gly Tyr Ser Thr Lys Pro Gln Leu Ala Ile Val 
        515                 520                 525             


Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr His His Leu His Ile Ile 
    530                 535                 540                 


Glu Thr Lys Phe Glu Met Ile Lys Leu Ile Asp Ile Ala Arg Gln Thr 
545                 550                 555                 560 


Ala Gln Gly Met Asp Tyr Leu His Ala Lys Ser Ile Ile His Arg Asp 
                565                 570                 575     


Leu Lys Ser Asn Asn Ile Phe Leu His Glu Asp Leu Thr Val Lys Ile 
            580                 585                 590         


Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser His 
        595                 600                 605             


Gln Phe Glu Gln Leu Ser Gly Ser Ile Leu Trp Met Ala Pro Glu Val 
    610                 615                 620                 


Ile Arg Met Gln Asp Lys Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr 
625                 630                 635                 640 


Ala Phe Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Gln Leu Pro Tyr 
                645                 650                 655     


Ser Asn Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly 
            660                 665                 670         


Tyr Leu Ser Pro Asp Leu Ser Lys Val Arg Ser Asn Cys Pro Lys Ala 
        675                 680                 685             


Met Lys Arg Leu Met Ala Glu Cys Leu Lys Lys Lys Arg Asp Glu Arg 
    690                 695                 700                 


Pro Leu Phe Pro Gln Ile Leu Ala Ser Ile Glu Leu Leu Ala Arg Ser 
705                 710                 715                 720 


Leu Pro Lys Ile His Arg Ser Ala Ser Glu Pro Ser Leu Asn Arg Ala 
                725                 730                 735     


Gly Phe Gln Thr Glu Asp Phe Ser Leu Tyr Ala Cys Ala Ser Pro Lys 
            740                 745                 750         


Thr Pro Ile Gln Ala Gly Gly Tyr Gly Ala Phe Pro Val His 
        755                 760                 765     




