                         SEQUENCE LISTING

<110>  Protox Therqpeutics Corporation
 
<120>  USE OF HUMAN SERUM ALBUMIN TO DECREASE ANTIGENICITY OF 
       THERAPEUTIC PROTEINS

<130>  PTX0001-401-PC

<150>  61/406,052
<151>  2010-10-22

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  1410
<212>  DNA
<213>  Aeromonas hydrophilia

<220>
<221>  CDS
<222>  (1)..(1410)
<223>  


<400>  1
gca gag ccc gtc tat cca gac cag ctt cgc ttg ttt tca ttg ggc caa       48
Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln         
1               5                   10                  15              

ggg gtc tgt ggc gac aag tat cgc ccc gtc aat cga gaa gaa gcc caa       96
Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln         
            20                  25                  30                  

agc gtt aaa agc aat att gtc ggc atg atg ggg caa tgg caa ata agc      144
Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser         
        35                  40                  45                      

ggg ctg gcc aac ggc tgg gtc att atg ggg ccg ggt tat aac ggt gaa      192
Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu         
    50                  55                  60                          

ata aaa cca ggg aca gcg tcc aat acc tgg tgt tat ccg acc aat cct      240
Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro         
65                  70                  75                  80          

gtt acc ggt gaa ata ccg aca ctg tct gcc ctg gat att cca gat ggt      288
Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly         
                85                  90                  95              

gac gaa gtc gat gtg cag tgg cga ctg gta cat gac agt gcg aat ttc      336
Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe         
            100                 105                 110                 

atc aaa cca acc agc tat ctg gcc cat tac ctc ggt tat gcc tgg gtg      384
Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val         
        115                 120                 125                     

ggc ggc aat cac agc caa tat gtc ggc gaa gac atg gat gtg acc cgt      432
Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg         
    130                 135                 140                         

gat ggc gac ggc tgg gtg atc cgt ggc aac aat gac ggc ggc tgt gac      480
Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp         
145                 150                 155                 160         

ggc tat cgc tgt ggt gac aag acg gcc atc aag gtc agc aac ttc gcc      528
Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala         
                165                 170                 175             

tat aac ctg gat ccc gac agc ttc aag cat ggc gat gtc acc cag tcc      576
Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser         
            180                 185                 190                 

gac cgc cag ctg gtc aag act gtg gtg ggc tgg gcg gtc aac gac agc      624
Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser         
        195                 200                 205                     

gac acc ccc caa tcc ggc tat gac gtc acc ctg cgc tac gac aca gcc      672
Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala         
    210                 215                 220                         

acc aac tgg tcc aag acc aac acc tat ggc ctg agc gag aag gtg acc      720
Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr         
225                 230                 235                 240         

acc aag aac aag ttc aag tgg cca ctg gtg ggg gaa acc caa ctc tcc      768
Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser         
                245                 250                 255             

atc gag att gct gcc aat cag tcc tgg gcg tcc cag aac ggg ggc tcg      816
Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser         
            260                 265                 270                 

acc acc acc tcc ctg tct cag tcc gtg cga ccg act gtg ccg gcc cgc      864
Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg         
        275                 280                 285                     

tcc aag atc ccg gtg aag ata gag ctc tac aag gcc gac atc tcc tat      912
Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr         
    290                 295                 300                         

ccc tat gag ttc aag gcc gat gtc agc tat gac ctg acc ctg agc ggc      960
Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly         
305                 310                 315                 320         

ttc ctg cgc tgg ggc ggc aac gcc tgg tat acc cac ccg gac aac cgt     1008
Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg         
                325                 330                 335             

ccg aac tgg aac cac acc ttc gtc ata ggt ccg tac aag gac aag gcg     1056
Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala         
            340                 345                 350                 

agc agc att cgg tac cag tgg gac aag cgt tac atc ccg ggt gaa gtg     1104
Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val         
        355                 360                 365                     

aag tgg tgg gac tgg aac tgg acc ata cag cag aac ggt ctg tct acc     1152
Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr         
    370                 375                 380                         

atg cag aac aac ctg gcc aga gtg ctg cgc ccg gtg cgg gcg ggg atc     1200
Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile         
385                 390                 395                 400         

acc ggt gat ttc agt gcc gag agc cag ttt gcc ggc aac ata gag atc     1248
Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile         
                405                 410                 415             

ggt gct ccc gtg ccg ctc gcg gct gac agc aag gtg cgt cgt gct cgc     1296
Gly Ala Pro Val Pro Leu Ala Ala Asp Ser Lys Val Arg Arg Ala Arg         
            420                 425                 430                 

agt gtg gac ggc gct ggt caa ggc ctg agg ctg gag atc ccg ctc gat     1344
Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp         
        435                 440                 445                     

gcg caa gag ctc tcc ggg ctt ggc ttc aac aac gtc agc ctc agc gtg     1392
Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val         
    450                 455                 460                         

acc cct gct gcc aat caa                                             1410
Thr Pro Ala Ala Asn Gln                                                 
465                 470                                                 


<210>  2
<211>  470
<212>  PRT
<213>  Aeromonas hydrophilia

<400>  2

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Gln Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser Lys Val Arg Arg Ala Arg 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 


<210>  3
<211>  1410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<220>
<221>  CDS
<222>  (1)..(1410)
<223>  


<400>  3
gca gag ccc gtc tat cca gac cag ctt cgc ttg ttt tca ttg ggc caa       48
Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln         
1               5                   10                  15              

ggg gtc tgt ggc gac aag tat cgc ccc gtc aat cga gaa gaa gcc caa       96
Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln         
            20                  25                  30                  

agc gtt aaa agc aat att gtc ggc atg atg ggg caa tgg caa ata agc      144
Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser         
        35                  40                  45                      

ggg ctg gcc aac ggc tgg gtc att atg ggg ccg ggt tat aac ggt gaa      192
Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu         
    50                  55                  60                          

ata aaa cca ggg aca gcg tcc aat acc tgg tgt tat ccg acc aat cct      240
Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro         
65                  70                  75                  80          

gtt acc ggt gaa ata ccg aca ctg tct gcc ctg gat att cca gat ggt      288
Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly         
                85                  90                  95              

gac gaa gtc gat gtg cag tgg cga ctg gta cat gac agt gcg aat ttc      336
Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe         
            100                 105                 110                 

atc aaa cca acc agc tat ctg gcc cat tac ctc ggt tat gcc tgg gtg      384
Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val         
        115                 120                 125                     

ggc ggc aat cac agc caa tat gtc ggc gaa gac atg gat gtg acc cgt      432
Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg         
    130                 135                 140                         

gat ggc gac ggc tgg gtg atc cgt ggc aac aat gac ggc ggc tgt gac      480
Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp         
145                 150                 155                 160         

ggc tat cgc tgt ggt gac aag acg gcc atc aag gtc agc aac ttc gcc      528
Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala         
                165                 170                 175             

tat aac ctg gat ccc gac agc ttc aag cat ggc gat gtc acc cag tcc      576
Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser         
            180                 185                 190                 

gac cgc cag ctg gtc aag act gtg gtg ggc tgg gcg gtc aac gac agc      624
Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser         
        195                 200                 205                     

gac acc ccc caa tcc ggc tat gac gtc acc ctg cgc tac gac aca gcc      672
Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala         
    210                 215                 220                         

acc aac tgg tcc aag acc aac acc tat ggc ctg agc gag aag gtg acc      720
Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr         
225                 230                 235                 240         

acc aag aac aag ttc aag tgg cca ctg gtg ggg gaa acc caa ctc tcc      768
Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser         
                245                 250                 255             

atc gag att gct gcc aat cag tcc tgg gcg tcc cag aac ggg ggc tcg      816
Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser         
            260                 265                 270                 

acc acc acc tcc ctg tct cag tcc gtg cga ccg act gtg ccg gcc cgc      864
Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg         
        275                 280                 285                     

tcc aag atc ccg gtg aag ata gag ctc tac aag gcc gac atc tcc tat      912
Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr         
    290                 295                 300                         

ccc tat gag ttc aag gcc gat gtc agc tat gac ctg acc ctg agc ggc      960
Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly         
305                 310                 315                 320         

ttc ctg cgc tgg ggc ggc aac gcc tgg tat acc cac ccg gac aac cgt     1008
Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg         
                325                 330                 335             

ccg aac tgg aac cac acc ttc gtc ata ggt ccg tac aag gac aag gcg     1056
Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala         
            340                 345                 350                 

agc agc att cgg tac cag tgg gac aag cgt tac atc ccg ggt gaa gtg     1104
Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val         
        355                 360                 365                     

aag tgg tgg gac tgg aac tgg acc ata cag cag aac ggt ctg tct acc     1152
Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr         
    370                 375                 380                         

atg cag aac aac ctg gcc aga gtg ctg cgc ccg gtg cgg gcg ggg atc     1200
Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile         
385                 390                 395                 400         

acc ggt gat ttc agt gcc gag agc cag ttt gcc ggc aac ata gag atc     1248
Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile         
                405                 410                 415             

ggt gct ccc gtg ccg ctc gcg gct gac agc cat tcc tcc aag ctg cag     1296
Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln         
            420                 425                 430                 

agt gtg gac ggc gct ggt caa ggc ctg agg ctg gag atc ccg ctc gat     1344
Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp         
        435                 440                 445                     

gcg caa gag ctc tcc ggg ctt ggc ttc aac aac gtc agc ctc agc gtg     1392
Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val         
    450                 455                 460                         

acc cct gct gcc aat caa                                             1410
Thr Pro Ala Ala Asn Gln                                                 
465                 470                                                 


<210>  4
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site.

<400>  4

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln 
465                 470 

<210>  5
<211>  6
<212>  PRT
<213>  Homo Sapiens

<400>  5

His Ser Ser Lys Leu Gln 
1               5       

<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA cleavage site

<400>  6

His Ser Ser Lys Leu Gln Ser Ala 
1               5               
<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223> PSA cleavage site 

<400>  7

Gln Phe Tyr Ser Ser Asn 
1               5       


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223> PSA cleavage site 

<400>  8

Gly Ile Ser Ser Phe Gln Ser 
1               5           


<210>  9
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  9

Lys Gly Ile Ser Ser Gln Tyr 
1               5           


<210>  10
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  10

Ser Arg Lys Ser Gln Gln Tyr 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  11

Ala Thr Lys Ser Lys Gln His 
1               5           


<210>  12
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  12

Lys Gly Leu Ser Ser Gln Cys 
1               5           


<210>  13
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  13

Leu Gly Gly Ser Ser Gln Leu 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  14

Glu His Ser Ser Lys Leu Gln 
1               5           


<210>  15
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  15

Ser Lys Leu Gln 
1               

<210>  16
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  16

Gln His Trp Ser Tyr Gly Leu Arg Pro Gly 
1               5                   10  


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LHRH variant sequence.

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Glu is a pyroglutamate


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Lys is a D-Lys


<400>  17

Glu His Trp Ser Tyr Lys Leu Arg Pro Gly 
1               5                   10  


<210> 18
<211> 1236
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: genetic fusion

<220> 
<221> CDS
<222> (1)..(1236)

<400> 18
atg ggc cat cat cat cat cat cat cat cat cat cac agc agc ggc cat   48    
Met Gly His His His His His His His His His His Ser Ser Gly His
  1               5                  10                  15    

atc gaa ggt cgt atg tct cag agc aac cgg gag ctg gtg gtt gac ttt   96    
Ile Glu Gly Arg Met Ser Gln Ser Asn Arg Glu Leu Val Val Asp Phe
             20                  25                  30        

ctc tcc tac aag ctt tcc cag aaa gga tac agc tgg agt cag ttt agt   144   
Leu Ser Tyr Lys Leu Ser Gln Lys Gly Tyr Ser Trp Ser Gln Phe Ser
         35                  40                  45            

gat gtg gaa gag aac agg act gag gcc cca gaa ggg act gaa tcg gag   192   
Asp Val Glu Glu Asn Arg Thr Glu Ala Pro Glu Gly Thr Glu Ser Glu
     50                  55                  60                

atg gag acc ccc agt gcc atc aat ggc aac cca tcc tgg cac ctg gca   240   
Met Glu Thr Pro Ser Ala Ile Asn Gly Asn Pro Ser Trp His Leu Ala
 65                  70                  75                  80

gac agc ccc gcg gtg aat gga gcc act gcg cac agc agc agt ttg gat   288   
Asp Ser Pro Ala Val Asn Gly Ala Thr Ala His Ser Ser Ser Leu Asp
                 85                  90                  95    

gcc cgg gag gtg atc ccc atg gca gca gta aag caa gcg ctg agg gag   336   
Ala Arg Glu Val Ile Pro Met Ala Ala Val Lys Gln Ala Leu Arg Glu
            100                 105                 110        

gca ggc gac gag ttt gaa ctg cgg tac cgg cgg gca ttc agt gac ctg   384   
Ala Gly Asp Glu Phe Glu Leu Arg Tyr Arg Arg Ala Phe Ser Asp Leu
        115                 120                 125            

aca tcc cag ctc cac atc acc cca ggg aca gca tat cag agc ttt gaa   432   
Thr Ser Gln Leu His Ile Thr Pro Gly Thr Ala Tyr Gln Ser Phe Glu
    130                 135                 140                

cag gta gtg aat gaa ctc ttc cgg gat ggg gta aac tgg ggt cgc att   480   
Gln Val Val Asn Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile
145                 150                 155                 160

gtg gcc ttt ttc tcc ttc ggc ggg gca ctg tgc gtg gaa agc gta gac   528   
Val Ala Phe Phe Ser Phe Gly Gly Ala Leu Cys Val Glu Ser Val Asp
                165                 170                 175    

aag gag atg cag gta ttg gtg agt cgg atc gca gct tgg atg gcc act   576   
Lys Glu Met Gln Val Leu Val Ser Arg Ile Ala Ala Trp Met Ala Thr
            180                 185                 190        

tac ctg aat gac cac cta gag cct tgg atc cag gag aac ggc ggc tgg   624   
Tyr Leu Asn Asp His Leu Glu Pro Trp Ile Gln Glu Asn Gly Gly Trp
        195                 200                 205            

gat act ttt gtg gaa ctc tat ggg aac aat gca gca gcc gag agc cga   672   
Asp Thr Phe Val Glu Leu Tyr Gly Asn Asn Ala Ala Ala Glu Ser Arg
    210                 215                 220                

aag ggc cag gaa cgc ttc aac cgc tgg ttc ctg acg ggc atg act gtg   720   
Lys Gly Gln Glu Arg Phe Asn Arg Trp Phe Leu Thr Gly Met Thr Val
225                 230                 235                 240

gcc ggc gtg gtt ctg ctg ggc tca ctc ttc agt cgg aaa gcg tat tct   768   
Ala Gly Val Val Leu Leu Gly Ser Leu Phe Ser Arg Lys Ala Tyr Ser
                245                 250                 255    

gcg gcc gcg cat aaa acg caa cca ttt ctt cat gac ggg tat gct gtc   816   
Ala Ala Ala His Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val
            260                 265                 270        

agt tgg aac act gtt gaa gat tcg ata atc cga act ggt ttt caa ggg   864   
Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly
        275                 280                 285            

gag agt ggg cac gac ata aaa att act gct gaa aat acc ccg ctt cca   912   
Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro
    290                 295                 300                

atc gcg ggt gtc cta cta ccg act att cct gga aag ctg gac gtt aat   960   
Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn
305                 310                 315                 320

aag tcc aag act cat att tcc gta aat ggt cgg aaa ata agg atg cgt   1008  
Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg
                325                 330                 335    

tgc aga gct ata gac ggt gat gta act ttt tgt cgc cct aaa tct cct   1056  
Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro
            340                 345                 350        

gtt tat gtt ggt aat ggt gtg cat gcg aat ctt cac gtg gca ttt cac   1104  
Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala Phe His
        355                 360                 365            

aga agc agc tcg gag aaa att cat tct aat gaa att tcg tcg gat tcc   1152  
Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser
    370                 375                 380                

ata ggc gtt ctt ggg tac cag aaa aca gta gat cac acc aag gtt aat   1200  
Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn
385                 390                 395                 400

tct aag cta tcg cta ttt ttt gaa atc aaa agc tga                   1236  
Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser    
                405                 410        


<210> 19
<211> 411        
<212> PRT
<213> Artificial Sequence
<223> Description of Artificial Sequence: genetic fusion

<400> 19
Met Gly His His His His His His His His His His Ser Ser Gly His  
  1               5                  10                  15      
Ile Glu Gly Arg Met Ser Gln Ser Asn Arg Glu Leu Val Val Asp Phe  
             20                  25                  30          
Leu Ser Tyr Lys Leu Ser Gln Lys Gly Tyr Ser Trp Ser Gln Phe Ser  
         35                  40                  45              
Asp Val Glu Glu Asn Arg Thr Glu Ala Pro Glu Gly Thr Glu Ser Glu  
     50                  55                  60                  
Met Glu Thr Pro Ser Ala Ile Asn Gly Asn Pro Ser Trp His Leu Ala  
 65                  70                  75                  80  
Asp Ser Pro Ala Val Asn Gly Ala Thr Ala His Ser Ser Ser Leu Asp  
                 85                  90                  95      
Ala Arg Glu Val Ile Pro Met Ala Ala Val Lys Gln Ala Leu Arg Glu  
            100                 105                 110          
Ala Gly Asp Glu Phe Glu Leu Arg Tyr Arg Arg Ala Phe Ser Asp Leu  
        115                 120                 125              
Thr Ser Gln Leu His Ile Thr Pro Gly Thr Ala Tyr Gln Ser Phe Glu  
    130                 135                 140                  
Gln Val Val Asn Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile  
145                 150                 155                 160  
Val Ala Phe Phe Ser Phe Gly Gly Ala Leu Cys Val Glu Ser Val Asp  
                165                 170                 175      
Lys Glu Met Gln Val Leu Val Ser Arg Ile Ala Ala Trp Met Ala Thr  
            180                 185                 190          
Tyr Leu Asn Asp His Leu Glu Pro Trp Ile Gln Glu Asn Gly Gly Trp  
        195                 200                 205              
Asp Thr Phe Val Glu Leu Tyr Gly Asn Asn Ala Ala Ala Glu Ser Arg  
    210                 215                 220                  
Lys Gly Gln Glu Arg Phe Asn Arg Trp Phe Leu Thr Gly Met Thr Val  
225                 230                 235                 240  
Ala Gly Val Val Leu Leu Gly Ser Leu Phe Ser Arg Lys Ala Tyr Ser  
                245                 250                 255      
Ala Ala Ala His Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val  
            260                 265                 270          
Ser Trp Asn Thr Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly  
        275                 280                 285              
Glu Ser Gly His Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro  
    290                 295                 300                  
Ile Ala Gly Val Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn  
305                 310                 315                 320  
Lys Ser Lys Thr His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg  
                325                 330                 335      
Cys Arg Ala Ile Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro  
            340                 345                 350          
Val Tyr Val Gly Asn Gly Val His Ala Asn Leu His Val Ala Phe His  
        355                 360                 365              
Arg Ser Ser Ser Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser  
    370                 375                 380                  
Ile Gly Val Leu Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn  
385                 390                 395                 400  
Ser Lys Leu Ser Leu Phe Phe Glu Ile Lys Ser                      
                405                 410                          



<210> 20
<211> 1704
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: genetic fusion

<220> 
<221> CDS
<222> (1)..(1704)

<400> 20
atg ggc cat cat cat cat cat cat cat cat cat cac agc agc ggc cat   48    
Met Gly His His His His His His His His His His Ser Ser Gly His
  1               5                  10                  15    

atc gaa ggt cgt cat atg gga acc cca aag cag ccc tcg ctg gct cct   96    
Ile Glu Gly Arg His Met Gly Thr Pro Lys Gln Pro Ser Leu Ala Pro
             20                  25                  30        

gca cac gcc cta ggc ttg agg aag tcc gat ccc gga atc cgg agc ctg   144   
Ala His Ala Leu Gly Leu Arg Lys Ser Asp Pro Gly Ile Arg Ser Leu
         35                  40                  45            

ggg agc gac gcg gga gga agg cgg tgg aga cca gca gcc cag agt atg   192   
Gly Ser Asp Ala Gly Gly Arg Arg Trp Arg Pro Ala Ala Gln Ser Met
     50                  55                  60                

ttc cag atc cca gag ttt gag ccg agt gag cag gaa gac gct agt gct   240   
Phe Gln Ile Pro Glu Phe Glu Pro Ser Glu Gln Glu Asp Ala Ser Ala
 65                  70                  75                  80

aca gat agg ggc ctg ggc cct agc ctc act gag gac cag cca ggt ccc   288   
Thr Asp Arg Gly Leu Gly Pro Ser Leu Thr Glu Asp Gln Pro Gly Pro
                 85                  90                  95    

tac ctg gcc cca ggt ctc ctg ggg agc aac att cat cag cag gga cgg   336   
Tyr Leu Ala Pro Gly Leu Leu Gly Ser Asn Ile His Gln Gln Gly Arg
            100                 105                 110        

gca gcc acc aac agt cat cat gga ggc gca ggg gct atg gag act cgg   384   
Ala Ala Thr Asn Ser His His Gly Gly Ala Gly Ala Met Glu Thr Arg
        115                 120                 125            

agt cgc cac agt gcg tac cca gcg ggg acc gag gag gat gaa ggg atg   432   
Ser Arg His Ser Ala Tyr Pro Ala Gly Thr Glu Glu Asp Glu Gly Met
    130                 135                 140                

gag gag gag ctt agc cct ttt cga gga cgc tcg cgt gcg gct ccc ccc   480   
Glu Glu Glu Leu Ser Pro Phe Arg Gly Arg Ser Arg Ala Ala Pro Pro
145                 150                 155                 160

aat ctc tgg gca gcg cag cgc tac ggc cgt gag ctc cga agg atg agc   528   
Asn Leu Trp Ala Ala Gln Arg Tyr Gly Arg Glu Leu Arg Arg Met Ser
                165                 170                 175    

gat gag ttt gag ggt tcc ttc aag gga ctt cct cgc cca aag agc gca   576   
Asp Glu Phe Glu Gly Ser Phe Lys Gly Leu Pro Arg Pro Lys Ser Ala
            180                 185                 190        

ggc act gca aca cag atg cga caa agc gcc ggc tgg acg cgc att atc   624   
Gly Thr Ala Thr Gln Met Arg Gln Ser Ala Gly Trp Thr Arg Ile Ile
        195                 200                 205            

cag tcc tgg tgg gat cga aac ttg ggc aaa gga ggc tcc acc ccc tcc   672   
Gln Ser Trp Trp Asp Arg Asn Leu Gly Lys Gly Gly Ser Thr Pro Ser
    210                 215                 220                

cag tca gta ggt agc tca ttg tca tgc ata aat ctt gat tgg gat gtc   720   
Gln Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val
225                 230                 235                 240

ata agg gat aaa act aag aca aag ata gag tct ttg aaa gag cat ggc   768   
Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly
                245                 250                 255    

cct atc aaa aat aaa atg agc gaa agt ccc aat aaa aca gta tct gag   816   
Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu
            260                 265                 270        

gaa aaa gct aaa caa tac cta gaa gaa ttt cat caa acg gca tta gag   864   
Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu
        275                 280                 285            

cat cct gaa ttg tca gaa ctt aaa acc gtt act ggg acc aat cct gta   912   
His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val
    290                 295                 300                

ttc gct ggg gct aac tat gcg gcg tgg gca gta aac gtt gcg caa gtt   960   
Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val
305                 310                 315                 320

atc gat agc gaa aca gct gat aat ttg gaa aag aca act gct gct ctt   1008  
Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu
                325                 330                 335    

tcg ata ctt cct ggt atc ggt agc gta atg ggc att gca gac ggt gcc   1056  
Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala
            340                 345                 350        

gtt cac cac aat aca gaa gag ata gtg gca caa tca ata gct tta tcg   1104  
Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser
        355                 360                 365            

tct tta atg gtt gct caa gct att cca ttg gta gga gag cta gtt gat   1152  
Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp
    370                 375                 380                

att ggt ttc gct gca tat aat ttt gta gag agt att atc aat tta ttt   1200  
Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe
385                 390                 395                 400

caa gta gtt cat aat tcg tat aat cgt ccc gcg tat tct ccg ggg cat   1248  
Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His
                405                 410                 415    

aaa acg caa cca ttt ctt cat gac ggg tat gct gtc agt tgg aac act   1296  
Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr
            420                 425                 430        

gtt gaa gat tcg ata atc cga act ggt ttt caa ggg gag agt ggg cac   1344  
Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His
        435                 440                 445            

gac ata aaa att act gct gaa aat acc ccg ctt cca atc gcg ggt gtc   1392  
Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val
    450                 455                 460                

cta cta ccg act att cct gga aag ctg gac gtt aat aag tcc aag act   1440  
Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr
465                 470                 475                 480

cat att tcc gta aat ggt cgg aaa ata agg atg cgt tgc aga gct ata   1488  
His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile
                485                 490                 495    

gac ggt gat gta act ttt tgt cgc cct aaa tct cct gtt tat gtt ggt   1536  
Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly
            500                 505                 510        

aat ggt gtg cat gcg aat ctt cac gtg gca ttt cac aga agc agc tcg   1584  
Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser
        515                 520                 525            

gag aaa att cat tct aat gaa att tcg tcg gat tcc ata ggc gtt ctt   1632  
Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu
    530                 535                 540                

ggg tac cag aaa aca gta gat cac acc aag gtt aat tct aag cta tcg   1680  
Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser
545                 550                 555                 560

cta ttt ttt gaa atc aaa agc tga                                   1704  
Leu Phe Phe Glu Ile Lys Ser    
                565            


<210> 21
<211> 567        
<212> PRT
<213> Artificial Sequence
<223> Description of Artificial Sequence: genetic fusion

<400> 21
Met Gly His His His His His His His His His His Ser Ser Gly His  
  1               5                  10                  15      
Ile Glu Gly Arg His Met Gly Thr Pro Lys Gln Pro Ser Leu Ala Pro  
             20                  25                  30          
Ala His Ala Leu Gly Leu Arg Lys Ser Asp Pro Gly Ile Arg Ser Leu  
         35                  40                  45              
Gly Ser Asp Ala Gly Gly Arg Arg Trp Arg Pro Ala Ala Gln Ser Met  
     50                  55                  60                  
Phe Gln Ile Pro Glu Phe Glu Pro Ser Glu Gln Glu Asp Ala Ser Ala  
 65                  70                  75                  80  
Thr Asp Arg Gly Leu Gly Pro Ser Leu Thr Glu Asp Gln Pro Gly Pro  
                 85                  90                  95      
Tyr Leu Ala Pro Gly Leu Leu Gly Ser Asn Ile His Gln Gln Gly Arg  
            100                 105                 110          
Ala Ala Thr Asn Ser His His Gly Gly Ala Gly Ala Met Glu Thr Arg  
        115                 120                 125              
Ser Arg His Ser Ala Tyr Pro Ala Gly Thr Glu Glu Asp Glu Gly Met  
    130                 135                 140                  
Glu Glu Glu Leu Ser Pro Phe Arg Gly Arg Ser Arg Ala Ala Pro Pro  
145                 150                 155                 160  
Asn Leu Trp Ala Ala Gln Arg Tyr Gly Arg Glu Leu Arg Arg Met Ser  
                165                 170                 175      
Asp Glu Phe Glu Gly Ser Phe Lys Gly Leu Pro Arg Pro Lys Ser Ala  
            180                 185                 190          
Gly Thr Ala Thr Gln Met Arg Gln Ser Ala Gly Trp Thr Arg Ile Ile  
        195                 200                 205              
Gln Ser Trp Trp Asp Arg Asn Leu Gly Lys Gly Gly Ser Thr Pro Ser  
    210                 215                 220                  
Gln Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val  
225                 230                 235                 240  
Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly  
                245                 250                 255      
Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu  
            260                 265                 270          
Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu  
        275                 280                 285              
His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val  
    290                 295                 300                  
Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val  
305                 310                 315                 320  
Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu  
                325                 330                 335      
Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala  
            340                 345                 350          
Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser  
        355                 360                 365              
Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp  
    370                 375                 380                  
Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe  
385                 390                 395                 400  
Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His  
                405                 410                 415      
Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr  
            420                 425                 430          
Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His  
        435                 440                 445              
Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val  
    450                 455                 460                  
Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr  
465                 470                 475                 480  
His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile  
                485                 490                 495      
Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly  
            500                 505                 510          
Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser  
        515                 520                 525              
Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu  
    530                 535                 540                  
Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser  
545                 550                 555                 560  
Leu Phe Phe Glu Ile Lys Ser                                      
                565                                              



<210> 22
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence:
      oligonucleotide linker

<400> 22
gcgtattctg cggccgcg                                               18    


<210> 23
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: oligopeptide
      linker

<400> 23
Ala Tyr Ser Ala Ala Ala                                          
  1               5    


<210> 24
<211> 1455
<212> DNA
<213> Artificial Sequence

<220> 
<223> Description of Artificial Sequence: genetic fusion

<220> 
<221> CDS
<222> (1)..(1455)

<400> 24
atg ggc agc agc cat cat cat cat cat cac agc agc ggc ctg gtg ccg   48    
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro
  1               5                  10                  15    

cgc ggc agc cat atg gcg ggc ggt cat ggt gat gta ggt atg cac gta   96    
Arg Gly Ser His Met Ala Gly Gly His Gly Asp Val Gly Met His Val
             20                  25                  30        

aaa gag aaa gag aaa aat aaa gat gag aat aag aga aaa gat gaa gaa   144   
Lys Glu Lys Glu Lys Asn Lys Asp Glu Asn Lys Arg Lys Asp Glu Glu
         35                  40                  45            

cga aat aaa aca cag gaa gag cat tta aag gaa atc atg aaa cac att   192   
Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys His Ile
     50                  55                  60                

gta aaa ata gaa gta aaa ggg gag gaa gct gtt aaa aaa gag gca gca   240   
Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu Ala Ala
 65                  70                  75                  80

gaa aag cta ctt gag aaa gta cca tct gat gtt tta gag atg tat aaa   288   
Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met Tyr Lys
                 85                  90                  95    

gca att gga gga aag ata tat att gtg gat ggt gat att aca aaa cat   336   
Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr Lys His
            100                 105                 110        

ata tct tta gaa gca tta tct gaa gat aag aaa aaa ata aaa gac att   384   
Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys Asp Ile
        115                 120                 125            

tat ggg aaa gat gct tta tta cat gaa cat tat gta tat gca aaa gaa   432   
Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala Lys Glu
    130                 135                 140                

gga tat gaa ccc gta ctt gta atc caa tct tcg gaa gat tat gta gaa   480   
Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr Val Glu
145                 150                 155                 160

aat act gaa aag gca ctg aac gtt tat tat gaa ata ggt aag ata tta   528   
Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys Ile Leu
                165                 170                 175    

tca agg gat att tta agt aaa att aat caa cca tat cag aaa ttt tta   576   
Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys Phe Leu
            180                 185                 190        

gat gta tta aat acc att aaa aat gca tct gat tca gat gga caa gat   624   
Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly Gln Asp
        195                 200                 205            

ctt tta ttt act aat cag ctt aag gaa cat ccc aca gac ttt tct gta   672   
Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe Ser Val
    210                 215                 220                

gaa ttc ttg gaa caa aat agc aat gag gta caa gaa gta ttt gcg aaa   720   
Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe Ala Lys
225                 230                 235                 240

gct ttt gca tat tat atc gag cca cag cat cgt gat gtt tta cag ctt   768   
Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu Gln Leu
                245                 250                 255    

tat gca ccg gaa gct ttt aat tac atg gat aaa ttt aac gaa caa gaa   816   
Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu Gln Glu
            260                 265                 270        

ata aat cta tcc atg tct cag agc aac cgg gag ctg gtg gtt gac ttt   864   
Ile Asn Leu Ser Met Ser Gln Ser Asn Arg Glu Leu Val Val Asp Phe
        275                 280                 285            

ctc tcc tac aag ctt tcc cag aaa gga tac agc tgg agt cag ttt agt   912   
Leu Ser Tyr Lys Leu Ser Gln Lys Gly Tyr Ser Trp Ser Gln Phe Ser
    290                 295                 300                

gat gtg gaa gag aac agg act gag gcc cca gaa ggg act gaa tcg gag   960   
Asp Val Glu Glu Asn Arg Thr Glu Ala Pro Glu Gly Thr Glu Ser Glu
305                 310                 315                 320

atg gag acc ccc agt gcc atc aat ggc aac cca tcc tgg cac ctg gca   1008  
Met Glu Thr Pro Ser Ala Ile Asn Gly Asn Pro Ser Trp His Leu Ala
                325                 330                 335    

gac agc ccc gcg gtg aat gga gcc act gcg cac agc agc agt ttg gat   1056  
Asp Ser Pro Ala Val Asn Gly Ala Thr Ala His Ser Ser Ser Leu Asp
            340                 345                 350        

gcc cgg gag gtg atc ccc atg gca gca gta aag caa gcg ctg agg gag   1104  
Ala Arg Glu Val Ile Pro Met Ala Ala Val Lys Gln Ala Leu Arg Glu
        355                 360                 365            

gca ggc gac gag ttt gaa ctg cgg tac cgg cgg gca ttc agt gac ctg   1152  
Ala Gly Asp Glu Phe Glu Leu Arg Tyr Arg Arg Ala Phe Ser Asp Leu
    370                 375                 380                

aca tcc cag ctc cac atc acc cca ggg aca gca tat cag agc ttt gaa   1200  
Thr Ser Gln Leu His Ile Thr Pro Gly Thr Ala Tyr Gln Ser Phe Glu
385                 390                 395                 400

cag gta gtg aat gaa ctc ttc cgg gat ggg gta aac tgg ggt cgc att   1248  
Gln Val Val Asn Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile
                405                 410                 415    

gtg gcc ttt ttc tcc ttc ggc ggg gca ctg tgc gtg gaa agc gta gac   1296  
Val Ala Phe Phe Ser Phe Gly Gly Ala Leu Cys Val Glu Ser Val Asp
            420                 425                 430        

aag gag atg cag gta ttg gtg agt cgg atc gca gct tgg atg gcc act   1344  
Lys Glu Met Gln Val Leu Val Ser Arg Ile Ala Ala Trp Met Ala Thr
        435                 440                 445            

tac ctg aat gac cac cta gag cct tgg atc cag gag aac ggc ggc tgg   1392  
Tyr Leu Asn Asp His Leu Glu Pro Trp Ile Gln Glu Asn Gly Gly Trp
    450                 455                 460                

gat act ttt gtg gaa ctc tat ggg aac aat gca gca gcc gag agc cga   1440  
Asp Thr Phe Val Glu Leu Tyr Gly Asn Asn Ala Ala Ala Glu Ser Arg
465                 470                 475                 480

aag ggc cag gaa cgc                                               1455  
Lys Gly Gln Glu Arg
                485


<210> 25
<211> 485
<212> PRT
<213> Artificial Sequence
<223> Description of Artificial Sequence: genetic fusion

<400> 25
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro  
  1               5                  10                  15      

Arg Gly Ser His Met Ala Gly Gly His Gly Asp Val Gly Met His Val  
             20                  25                  30          

Lys Glu Lys Glu Lys Asn Lys Asp Glu Asn Lys Arg Lys Asp Glu Glu  
         35                  40                  45              

Arg Asn Lys Thr Gln Glu Glu His Leu Lys Glu Ile Met Lys His Ile  
     50                  55                  60                  

Val Lys Ile Glu Val Lys Gly Glu Glu Ala Val Lys Lys Glu Ala Ala  
 65                  70                  75                  80  

Glu Lys Leu Leu Glu Lys Val Pro Ser Asp Val Leu Glu Met Tyr Lys  
                 85                  90                  95      

Ala Ile Gly Gly Lys Ile Tyr Ile Val Asp Gly Asp Ile Thr Lys His  
            100                 105                 110          

Ile Ser Leu Glu Ala Leu Ser Glu Asp Lys Lys Lys Ile Lys Asp Ile  
        115                 120                 125              

Tyr Gly Lys Asp Ala Leu Leu His Glu His Tyr Val Tyr Ala Lys Glu  
    130                 135                 140                  

Gly Tyr Glu Pro Val Leu Val Ile Gln Ser Ser Glu Asp Tyr Val Glu  
145                 150                 155                 160  

Asn Thr Glu Lys Ala Leu Asn Val Tyr Tyr Glu Ile Gly Lys Ile Leu  
                165                 170                 175      

Ser Arg Asp Ile Leu Ser Lys Ile Asn Gln Pro Tyr Gln Lys Phe Leu  
            180                 185                 190          

Asp Val Leu Asn Thr Ile Lys Asn Ala Ser Asp Ser Asp Gly Gln Asp  
        195                 200                 205              

Leu Leu Phe Thr Asn Gln Leu Lys Glu His Pro Thr Asp Phe Ser Val  
    210                 215                 220                  

Glu Phe Leu Glu Gln Asn Ser Asn Glu Val Gln Glu Val Phe Ala Lys  
225                 230                 235                 240  

Ala Phe Ala Tyr Tyr Ile Glu Pro Gln His Arg Asp Val Leu Gln Leu  
                245                 250                 255      

Tyr Ala Pro Glu Ala Phe Asn Tyr Met Asp Lys Phe Asn Glu Gln Glu  
            260                 265                 270          

Ile Asn Leu Ser Met Ser Gln Ser Asn Arg Glu Leu Val Val Asp Phe  
        275                 280                 285              

Leu Ser Tyr Lys Leu Ser Gln Lys Gly Tyr Ser Trp Ser Gln Phe Ser  
    290                 295                 300                  

Asp Val Glu Glu Asn Arg Thr Glu Ala Pro Glu Gly Thr Glu Ser Glu  
305                 310                 315                 320  

Met Glu Thr Pro Ser Ala Ile Asn Gly Asn Pro Ser Trp His Leu Ala  
                325                 330                 335      

Asp Ser Pro Ala Val Asn Gly Ala Thr Ala His Ser Ser Ser Leu Asp  
            340                 345                 350          

Ala Arg Glu Val Ile Pro Met Ala Ala Val Lys Gln Ala Leu Arg Glu  
        355                 360                 365              

Ala Gly Asp Glu Phe Glu Leu Arg Tyr Arg Arg Ala Phe Ser Asp Leu  
    370                 375                 380                  

Thr Ser Gln Leu His Ile Thr Pro Gly Thr Ala Tyr Gln Ser Phe Glu  
385                 390                 395                 400  

Gln Val Val Asn Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile  
                405                 410                 415      

Val Ala Phe Phe Ser Phe Gly Gly Ala Leu Cys Val Glu Ser Val Asp  
            420                 425                 430          

Lys Glu Met Gln Val Leu Val Ser Arg Ile Ala Ala Trp Met Ala Thr  
        435                 440                 445              

Tyr Leu Asn Asp His Leu Glu Pro Trp Ile Gln Glu Asn Gly Gly Trp  
    450                 455                 460                  

Asp Thr Phe Val Glu Leu Tyr Gly Asn Asn Ala Ala Ala Glu Ser Arg  
465                 470                 475                 480  

Lys Gly Gln Glu Arg                                              
                485 


<210>  26
<211>  1757
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-4 linked to Pseudomonas exotoxin

<400>  26
gcttgcatgc ctgcagaaga aggagatata catatgcaaa aaataaaact aactggcttg       60

tcattaatca tatccggcct gctgatggca caggcgcaag cggcagagcc cgtctatcca      120

gaccagcttc gcttgttttc attgggccaa ggggtctgtg gcgacaagta tcgccccgtc      180

aatcgagaag aagcccaaag cgttaaaagc aatattgtcg gcatgatggg gcaatggcaa      240

ataagcgggc tggccaacgg ctgggtcatt atggggccgg gttataacgg tgaaataaaa      300

ccagggacag cgtccaatac ctggtgttat ccgaccaatc ctgttaccgg tgaaataccg      360

acactgtctg ccctggatat tccagatggt gacgaagtcg atgtgcagtg gcgactggta      420

catgacagtg cgaatttcat caaaccaacc agctatctgg cccattacct cggttatgcc      480

tgggtgggcg gcaatcacag ccaatatgtc ggcgaagaca tggatgtgac ccgtgatggc      540

gacggctggg tgatccgtgg caacaatgac ggcggctgtg acggctatcg ctgtggtgac      600

aagacggcca tcaaggtcag caacttcgcc tataacctag atcccgacag cttcaagcat      660

ggcgatgtca cccagtccga ccgccagctg gtcaagactg tggtgggctg ggcggtcaac      720

gacagcgaca ccccccaatc cggctatgac gtcaccctgc gctacgacac agccaccaac      780

tggtccaaga ccaacaccta tggcctgagc gagaaggtga ccaccaagaa caagttcaag      840

tggccactgg tgggggaaac cgaactctcc atcgagattg ctgccaatca gtcctgggcg      900

tcccagaacg ggggctcgac caccacctcc ctgtctcagt ccgtgcgacc gactgtgccg      960

gcccgctcca agatcccggt gaagatagag ctctacaagg ccgacatctc ctatccctat     1020

gagttcaagg ccgatgtcag ctatgacctg accctgagcg gcttcctgcg ctggggcggc     1080

aacgcctggt atacccaccc ggacaaccgt ccgaactgga accacacctt cgtcataggt     1140

ccgtacaagg acaaggcgag cagcattcgg taccagtggg acaagcgtta catcccgggt     1200

gaagtgaagt ggtgggactg gaactggacc atacagcaga acggtctgtc taccatgcag     1260

aacaacctgg ccagagtgct gcgcccggtg cgggcgggga tcaccggtga tttcagtgcc     1320

gagagccagt ttgccggcaa catagagatc ggtgctcccg tgccgctcgc ggctgacagc     1380

cattcctcca agctgcagag tgtggacggc gctggtcaag gcctgaggct ggagatcccg     1440

ctcgatgcgc aagagctctc cgggcttggc ttcaacaacg tcagcctcag cgtgacccct     1500

gctgccaatc aacatcatca tcatcatcat taacggcagc gctaacaaca tcatcatcat     1560

catcattaac ggcagcgcgt tgtagtgatg gaaccgggcc tctggcccgg tttttgtttg     1620

cactggtcgg gcttgttaaa ggcttgtgct ttccatttcc ccacttatac tggcgccatc     1680

ttgtcggagt gccaaccgtc gaacgacgcg aggctgagac cgttaattcg ggatccgtgg     1740

aacctgatcc ccgggaa                                                    1757


<210>  27
<211>  1757
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-4 linked to Pseudomonas exotoxin

<400>  27

Gly Cys Thr Thr Gly Cys Ala Thr Gly Cys Cys Thr Gly Cys Ala Gly 
1               5                   10                  15      


Ala Ala Gly Ala Ala Gly Gly Ala Gly Ala Thr Ala Thr Ala Cys Ala 
            20                  25                  30          


Thr Ala Thr Gly Cys Ala Ala Ala Ala Ala Ala Thr Ala Ala Ala Ala 
        35                  40                  45              


Cys Thr Ala Ala Cys Thr Gly Gly Cys Thr Thr Gly Thr Cys Ala Thr 
    50                  55                  60                  


Thr Ala Ala Thr Cys Ala Thr Ala Thr Cys Cys Gly Gly Cys Cys Thr 
65                  70                  75                  80  


Gly Cys Thr Gly Ala Thr Gly Gly Cys Ala Cys Ala Gly Gly Cys Gly 
                85                  90                  95      


Cys Ala Ala Gly Cys Gly Gly Cys Ala Gly Ala Gly Cys Cys Cys Gly 
            100                 105                 110         


Thr Cys Thr Ala Thr Cys Cys Ala Gly Ala Cys Cys Ala Gly Cys Thr 
        115                 120                 125             


Thr Cys Gly Cys Thr Thr Gly Thr Thr Thr Thr Cys Ala Thr Thr Gly 
    130                 135                 140                 


Gly Gly Cys Cys Ala Ala Gly Gly Gly Gly Thr Cys Thr Gly Thr Gly 
145                 150                 155                 160 


Gly Cys Gly Ala Cys Ala Ala Gly Thr Ala Thr Cys Gly Cys Cys Cys 
                165                 170                 175     


Cys Gly Thr Cys Ala Ala Thr Cys Gly Ala Gly Ala Ala Gly Ala Ala 
            180                 185                 190         


Gly Cys Cys Cys Ala Ala Ala Gly Cys Gly Thr Thr Ala Ala Ala Ala 
        195                 200                 205             


Gly Cys Ala Ala Thr Ala Thr Thr Gly Thr Cys Gly Gly Cys Ala Thr 
    210                 215                 220                 


Gly Ala Thr Gly Gly Gly Gly Cys Ala Ala Thr Gly Gly Cys Ala Ala 
225                 230                 235                 240 


Ala Thr Ala Ala Gly Cys Gly Gly Gly Cys Thr Gly Gly Cys Cys Ala 
                245                 250                 255     


Ala Cys Gly Gly Cys Thr Gly Gly Gly Thr Cys Ala Thr Thr Ala Thr 
            260                 265                 270         


Gly Gly Gly Gly Cys Cys Gly Gly Gly Thr Thr Ala Thr Ala Ala Cys 
        275                 280                 285             


Gly Gly Thr Gly Ala Ala Ala Thr Ala Ala Ala Ala Cys Cys Ala Gly 
    290                 295                 300                 


Gly Gly Ala Cys Ala Gly Cys Gly Thr Cys Cys Ala Ala Thr Ala Cys 
305                 310                 315                 320 


Cys Thr Gly Gly Thr Gly Thr Thr Ala Thr Cys Cys Gly Ala Cys Cys 
                325                 330                 335     


Ala Ala Thr Cys Cys Thr Gly Thr Thr Ala Cys Cys Gly Gly Thr Gly 
            340                 345                 350         


Ala Ala Ala Thr Ala Cys Cys Gly Ala Cys Ala Cys Thr Gly Thr Cys 
        355                 360                 365             


Thr Gly Cys Cys Cys Thr Gly Gly Ala Thr Ala Thr Thr Cys Cys Ala 
    370                 375                 380                 


Gly Ala Thr Gly Gly Thr Gly Ala Cys Gly Ala Ala Gly Thr Cys Gly 
385                 390                 395                 400 


Ala Thr Gly Thr Gly Cys Ala Gly Thr Gly Gly Cys Gly Ala Cys Thr 
                405                 410                 415     


Gly Gly Thr Ala Cys Ala Thr Gly Ala Cys Ala Gly Thr Gly Cys Gly 
            420                 425                 430         


Ala Ala Thr Thr Thr Cys Ala Thr Cys Ala Ala Ala Cys Cys Ala Ala 
        435                 440                 445             


Cys Cys Ala Gly Cys Thr Ala Thr Cys Thr Gly Gly Cys Cys Cys Ala 
    450                 455                 460                 


Thr Thr Ala Cys Cys Thr Cys Gly Gly Thr Thr Ala Thr Gly Cys Cys 
465                 470                 475                 480 


Thr Gly Gly Gly Thr Gly Gly Gly Cys Gly Gly Cys Ala Ala Thr Cys 
                485                 490                 495     


Ala Cys Ala Gly Cys Cys Ala Ala Thr Ala Thr Gly Thr Cys Gly Gly 
            500                 505                 510         


Cys Gly Ala Ala Gly Ala Cys Ala Thr Gly Gly Ala Thr Gly Thr Gly 
        515                 520                 525             


Ala Cys Cys Cys Gly Thr Gly Ala Thr Gly Gly Cys Gly Ala Cys Gly 
    530                 535                 540                 


Gly Cys Thr Gly Gly Gly Thr Gly Ala Thr Cys Cys Gly Thr Gly Gly 
545                 550                 555                 560 


Cys Ala Ala Cys Ala Ala Thr Gly Ala Cys Gly Gly Cys Gly Gly Cys 
                565                 570                 575     


Thr Gly Thr Gly Ala Cys Gly Gly Cys Thr Ala Thr Cys Gly Cys Thr 
            580                 585                 590         


Gly Thr Gly Gly Thr Gly Ala Cys Ala Ala Gly Ala Cys Gly Gly Cys 
        595                 600                 605             


Cys Ala Thr Cys Ala Ala Gly Gly Thr Cys Ala Gly Cys Ala Ala Cys 
    610                 615                 620                 


Thr Thr Cys Gly Cys Cys Thr Ala Thr Ala Ala Cys Cys Thr Ala Gly 
625                 630                 635                 640 


Ala Thr Cys Cys Cys Gly Ala Cys Ala Gly Cys Thr Thr Cys Ala Ala 
                645                 650                 655     


Gly Cys Ala Thr Gly Gly Cys Gly Ala Thr Gly Thr Cys Ala Cys Cys 
            660                 665                 670         


Cys Ala Gly Thr Cys Cys Gly Ala Cys Cys Gly Cys Cys Ala Gly Cys 
        675                 680                 685             


Thr Gly Gly Thr Cys Ala Ala Gly Ala Cys Thr Gly Thr Gly Gly Thr 
    690                 695                 700                 


Gly Gly Gly Cys Thr Gly Gly Gly Cys Gly Gly Thr Cys Ala Ala Cys 
705                 710                 715                 720 


Gly Ala Cys Ala Gly Cys Gly Ala Cys Ala Cys Cys Cys Cys Cys Cys 
                725                 730                 735     


Ala Ala Thr Cys Cys Gly Gly Cys Thr Ala Thr Gly Ala Cys Gly Thr 
            740                 745                 750         


Cys Ala Cys Cys Cys Thr Gly Cys Gly Cys Thr Ala Cys Gly Ala Cys 
        755                 760                 765             


Ala Cys Ala Gly Cys Cys Ala Cys Cys Ala Ala Cys Thr Gly Gly Thr 
    770                 775                 780                 


Cys Cys Ala Ala Gly Ala Cys Cys Ala Ala Cys Ala Cys Cys Thr Ala 
785                 790                 795                 800 


Thr Gly Gly Cys Cys Thr Gly Ala Gly Cys Gly Ala Gly Ala Ala Gly 
                805                 810                 815     


Gly Thr Gly Ala Cys Cys Ala Cys Cys Ala Ala Gly Ala Ala Cys Ala 
            820                 825                 830         


Ala Gly Thr Thr Cys Ala Ala Gly Thr Gly Gly Cys Cys Ala Cys Thr 
        835                 840                 845             


Gly Gly Thr Gly Gly Gly Gly Gly Ala Ala Ala Cys Cys Gly Ala Ala 
    850                 855                 860                 


Cys Thr Cys Thr Cys Cys Ala Thr Cys Gly Ala Gly Ala Thr Thr Gly 
865                 870                 875                 880 


Cys Thr Gly Cys Cys Ala Ala Thr Cys Ala Gly Thr Cys Cys Thr Gly 
                885                 890                 895     


Gly Gly Cys Gly Thr Cys Cys Cys Ala Gly Ala Ala Cys Gly Gly Gly 
            900                 905                 910         


Gly Gly Cys Thr Cys Gly Ala Cys Cys Ala Cys Cys Ala Cys Cys Thr 
        915                 920                 925             


Cys Cys Cys Thr Gly Thr Cys Thr Cys Ala Gly Thr Cys Cys Gly Thr 
    930                 935                 940                 


Gly Cys Gly Ala Cys Cys Gly Ala Cys Thr Gly Thr Gly Cys Cys Gly 
945                 950                 955                 960 


Gly Cys Cys Cys Gly Cys Thr Cys Cys Ala Ala Gly Ala Thr Cys Cys 
                965                 970                 975     


Cys Gly Gly Thr Gly Ala Ala Gly Ala Thr Ala Gly Ala Gly Cys Thr 
            980                 985                 990         


Cys Thr Ala Cys Ala Ala Gly Gly  Cys Cys Gly Ala Cys  Ala Thr Cys 
        995                 1000                 1005             


Thr Cys  Cys Thr Ala Thr Cys  Cys Cys Thr Ala Thr  Gly Ala Gly 
    1010                 1015                 1020             


Thr Thr  Cys Ala Ala Gly Gly  Cys Cys Gly Ala Thr  Gly Thr Cys 
    1025                 1030                 1035             


Ala Gly  Cys Thr Ala Thr Gly  Ala Cys Cys Thr Gly  Ala Cys Cys 
    1040                 1045                 1050             


Cys Thr  Gly Ala Gly Cys Gly  Gly Cys Thr Thr Cys  Cys Thr Gly 
    1055                 1060                 1065             


Cys Gly  Cys Thr Gly Gly Gly  Gly Cys Gly Gly Cys  Ala Ala Cys 
    1070                 1075                 1080             


Gly Cys  Cys Thr Gly Gly Thr  Ala Thr Ala Cys Cys  Cys Ala Cys 
    1085                 1090                 1095             


Cys Cys  Gly Gly Ala Cys Ala  Ala Cys Cys Gly Thr  Cys Cys Gly 
    1100                 1105                 1110             


Ala Ala  Cys Thr Gly Gly Ala  Ala Cys Cys Ala Cys  Ala Cys Cys 
    1115                 1120                 1125             


Thr Thr  Cys Gly Thr Cys Ala  Thr Ala Gly Gly Thr  Cys Cys Gly 
    1130                 1135                 1140             


Thr Ala  Cys Ala Ala Gly Gly  Ala Cys Ala Ala Gly  Gly Cys Gly 
    1145                 1150                 1155             


Ala Gly  Cys Ala Gly Cys Ala  Thr Thr Cys Gly Gly  Thr Ala Cys 
    1160                 1165                 1170             


Cys Ala  Gly Thr Gly Gly Gly  Ala Cys Ala Ala Gly  Cys Gly Thr 
    1175                 1180                 1185             


Thr Ala  Cys Ala Thr Cys Cys  Cys Gly Gly Gly Thr  Gly Ala Ala 
    1190                 1195                 1200             


Gly Thr  Gly Ala Ala Gly Thr  Gly Gly Thr Gly Gly  Gly Ala Cys 
    1205                 1210                 1215             


Thr Gly  Gly Ala Ala Cys Thr  Gly Gly Ala Cys Cys  Ala Thr Ala 
    1220                 1225                 1230             


Cys Ala  Gly Cys Ala Gly Ala  Ala Cys Gly Gly Thr  Cys Thr Gly 
    1235                 1240                 1245             


Thr Cys  Thr Ala Cys Cys Ala  Thr Gly Cys Ala Gly  Ala Ala Cys 
    1250                 1255                 1260             


Ala Ala  Cys Cys Thr Gly Gly  Cys Cys Ala Gly Ala  Gly Thr Gly 
    1265                 1270                 1275             


Cys Thr  Gly Cys Gly Cys Cys  Cys Gly Gly Thr Gly  Cys Gly Gly 
    1280                 1285                 1290             


Gly Cys  Gly Gly Gly Gly Ala  Thr Cys Ala Cys Cys  Gly Gly Thr 
    1295                 1300                 1305             


Gly Ala  Thr Thr Thr Cys Ala  Gly Thr Gly Cys Cys  Gly Ala Gly 
    1310                 1315                 1320             


Ala Gly  Cys Cys Ala Gly Thr  Thr Thr Gly Cys Cys  Gly Gly Cys 
    1325                 1330                 1335             


Ala Ala  Cys Ala Thr Ala Gly  Ala Gly Ala Thr Cys  Gly Gly Thr 
    1340                 1345                 1350             


Gly Cys  Thr Cys Cys Cys Gly  Thr Gly Cys Cys Gly  Cys Thr Cys 
    1355                 1360                 1365             


Gly Cys  Gly Gly Cys Thr Gly  Ala Cys Ala Gly Cys  Cys Ala Thr 
    1370                 1375                 1380             


Thr Cys  Cys Thr Cys Cys Ala  Ala Gly Cys Thr Gly  Cys Ala Gly 
    1385                 1390                 1395             


Ala Gly  Thr Gly Thr Gly Gly  Ala Cys Gly Gly Cys  Gly Cys Thr 
    1400                 1405                 1410             


Gly Gly  Thr Cys Ala Ala Gly  Gly Cys Cys Thr Gly  Ala Gly Gly 
    1415                 1420                 1425             


Cys Thr  Gly Gly Ala Gly Ala  Thr Cys Cys Cys Gly  Cys Thr Cys 
    1430                 1435                 1440             


Gly Ala  Thr Gly Cys Gly Cys  Ala Ala Gly Ala Gly  Cys Thr Cys 
    1445                 1450                 1455             


Thr Cys  Cys Gly Gly Gly Cys  Thr Thr Gly Gly Cys  Thr Thr Cys 
    1460                 1465                 1470             


Ala Ala  Cys Ala Ala Cys Gly  Thr Cys Ala Gly Cys  Cys Thr Cys 
    1475                 1480                 1485             


Ala Gly  Cys Gly Thr Gly Ala  Cys Cys Cys Cys Thr  Gly Cys Thr 
    1490                 1495                 1500             


Gly Cys  Cys Ala Ala Thr Cys  Ala Ala Cys Ala Thr  Cys Ala Thr 
    1505                 1510                 1515             


Cys Ala  Thr Cys Ala Thr Cys  Ala Thr Cys Ala Thr  Thr Ala Ala 
    1520                 1525                 1530             


Cys Gly  Gly Cys Ala Gly Cys  Gly Cys Thr Ala Ala  Cys Ala Ala 
    1535                 1540                 1545             


Cys Ala  Thr Cys Ala Thr Cys  Ala Thr Cys Ala Thr  Cys Ala Thr 
    1550                 1555                 1560             


Cys Ala  Thr Thr Ala Ala Cys  Gly Gly Cys Ala Gly  Cys Gly Cys 
    1565                 1570                 1575             


Gly Thr  Thr Gly Thr Ala Gly  Thr Gly Ala Thr Gly  Gly Ala Ala 
    1580                 1585                 1590             


Cys Cys  Gly Gly Gly Cys Cys  Thr Cys Thr Gly Gly  Cys Cys Cys 
    1595                 1600                 1605             


Gly Gly  Thr Thr Thr Thr Thr  Gly Thr Thr Thr Gly  Cys Ala Cys 
    1610                 1615                 1620             


Thr Gly  Gly Thr Cys Gly Gly  Gly Cys Thr Thr Gly  Thr Thr Ala 
    1625                 1630                 1635             


Ala Ala  Gly Gly Cys Thr Thr  Gly Thr Gly Cys Thr  Thr Thr Cys 
    1640                 1645                 1650             


Cys Ala  Thr Thr Thr Cys Cys  Cys Cys Ala Cys Thr  Thr Ala Thr 
    1655                 1660                 1665             


Ala Cys  Thr Gly Gly Cys Gly  Cys Cys Ala Thr Cys  Thr Thr Gly 
    1670                 1675                 1680             


Thr Cys  Gly Gly Ala Gly Thr  Gly Cys Cys Ala Ala  Cys Cys Gly 
    1685                 1690                 1695             


Thr Cys  Gly Ala Ala Cys Gly  Ala Cys Gly Cys Gly  Ala Gly Gly 
    1700                 1705                 1710             


Cys Thr  Gly Ala Gly Ala Cys  Cys Gly Thr Thr Ala  Ala Thr Thr 
    1715                 1720                 1725             


Cys Gly  Gly Gly Ala Thr Cys  Cys Gly Thr Gly Gly  Ala Ala Cys 
    1730                 1735                 1740             


Cys Thr  Gly Ala Thr Cys Cys  Cys Cys Gly Gly Gly  Ala Ala 
    1745                 1750                 1755         


<210>  28
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Proaerolysin with a PSA sequence substituted for the furin site 
       and a polyhistidine tag

<400>  28

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala Asp Ser His Ser Ser Lys Leu Gln 
            420                 425                 430         


Ser Val Asp Gly Ala Gly Gln Gly Leu Arg Leu Glu Ile Pro Leu Asp 
        435                 440                 445             


Ala Gln Glu Leu Ser Gly Leu Gly Phe Asn Asn Val Ser Leu Ser Val 
    450                 455                 460                 


Thr Pro Ala Ala Asn Gln His His His His His His 
465                 470                 475     


