                         SEQUENCE LISTING

<110>  Laboratorios del Dr. Esteve S.A.
       Fundaci Privada Institut de Recerca de la SIDA-Caixa
 
<120>  VACCINE COMPOSITIONS BASED ON MODIFIED gp41 IMMUNOGENS

<130>  P6150PC00

<150>  EP10382284.7
<151>  2010-10-28


<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  PRT
<213>  Human immunodeficiency virus

<400>  1

Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala 
            20                  25                  30  


<210>  2
<211>  52
<212>  PRT
<213>  Human immunodeficiency virus

<400>  2

Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg 
1               5                   10                  15      


Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile 
            20                  25                  30          


Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp 
        35                  40                  45              


Gln Gln Leu Leu 
    50          


<210>  3
<211>  28
<212>  PRT
<213>  Human immunodeficiency virus

<400>  3

Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro 
1               5                   10                  15      


Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln 
            20                  25              


<210>  4
<211>  29
<212>  PRT
<213>  Human immunodeficiency virus

<400>  4

Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro 
1               5                   10                  15      


Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile 
            20                  25                  


<210>  5
<211>  29
<212>  PRT
<213>  Human immunodeficiency virus

<400>  5

Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro 
1               5                   10                  15      


Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile 
            20                  25                  


<210>  6
<211>  861
<212>  PRT
<213>  Human immunodeficiency virus

<400>  6

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Lys 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          



Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              



Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Gly Asn Ala Thr Asn Thr Asn Ser Ser Asn 
    130                 135                 140                 


Thr Asn Ser Ser Ser Gly Glu Met Met Met Glu Lys Gly Glu Ile Lys 
145                 150                 155                 160 


Asn Cys Ser Phe Asn Ile Ser Thr Ser Ile Arg Gly Lys Val Gln Lys 
                165                 170                 175     


Glu Tyr Ala Phe Phe Tyr Lys Leu Asp Ile Ile Pro Ile Asp Asn Asp 
            180                 185                 190         


Thr Thr Ser Tyr Thr Leu Thr Ser Cys Asn Thr Ser Val Ile Thr Gln 
        195                 200                 205             


Ala Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala 
    210                 215                 220                 


Pro Ala Gly Phe Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly 
225                 230                 235                 240 


Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
                245                 250                 255     



Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu 
            260                 265                 270         


Glu Glu Val Val Ile Arg Ser Ala Asn Phe Thr Asp Asn Ala Lys Thr 
        275                 280                 285             


Ile Ile Val Gln Leu Asn Gln Ser Val Glu Ile Asn Cys Thr Arg Pro 
    290                 295                 300                 


Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gln Arg Gly Pro Gly Arg 
305                 310                 315                 320 


Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met Arg Gln Ala His Cys 
                325                 330                 335     


Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu Lys Gln Ile Ala Ser 
            340                 345                 350         


Lys Leu Arg Glu Gln Phe Gly Asn Asn Lys Thr Ile Ile Phe Lys Gln 
        355                 360                 365             


Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly 
    370                 375                 380                 


Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp 
385                 390                 395                 400 


Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn Asn Thr Glu Gly Ser 
                405                 410                 415     


Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Phe Ile Asn Met Trp 
            420                 425                 430         


Gln Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile 
        435                 440                 445             


Arg Cys Ser Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly 
    450                 455                 460                 


Asn Asn Asn Asn Gly Ser Glu Ile Phe Arg Pro Gly Gly Gly Asp Met 
465                 470                 475                 480 


Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile 
                485                 490                 495     


Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln 
            500                 505                 510         


Arg Glu Lys Arg Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu 
        515                 520                 525             


Gly Ala Ala Gly Ser Thr Met Gly Ala Arg Ser Met Thr Leu Thr Val 
    530                 535                 540                 


Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu 
545                 550                 555                 560 


Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp 
                565                 570                 575     


Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu 
            580                 585                 590         


Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile 
        595                 600                 605             


Cys Thr Thr Ala Val Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu 
    610                 615                 620                 


Glu Gln Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile 
625                 630                 635                 640 


Asn Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn 
                645                 650                 655     


Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala 
            660                 665                 670         


Ser Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys 
        675                 680                 685             


Ile Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe 
    690                 695                 700                 


Ala Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu 
705                 710                 715                 720 


Ser Phe Gln Thr His Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu 
                725                 730                 735     


Gly Ile Glu Glu Glu Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg 
            740                 745                 750         


Leu Val Asn Gly Ser Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu 
        755                 760                 765             


Cys Leu Phe Ser Tyr His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr 
    770                 775                 780                 


Arg Ile Val Glu Leu Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr 
785                 790                 795                 800 


Trp Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala 
                805                 810                 815     


Val Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp 
            820                 825                 830         


Arg Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile 
        835                 840                 845             


Pro Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu Leu 
    850                 855                 860     


<210>  7
<211>  80
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN

<400>  7

Met Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
1               5                   10                  15      


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
            20                  25                  30          


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
        35                  40                  45              


Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu 
    50                  55                  60                  


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
65                  70                  75                  80  


<210>  8
<211>  133
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN with a tetrad glycine linker and a V5 vector epitope tag
       at the C-terminus

<400>  8

Met Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
1               5                   10                  15      


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
            20                  25                  30          


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
        35                  40                  45              


Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu 
    50                  55                  60                  


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
65                  70                  75                  80  


Val Leu Ser Ile Val Asn Arg Ala Gly Gly Gly Gly Lys Gly Gln Asp 
                85                  90                  95      


Asn Ser Ala Asp Ile Gln His Ser Gly Gly Arg Ser Ser Leu Glu Gly 
            100                 105                 110         


Pro Arg Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp 
        115                 120                 125             


Ser Thr Arg Thr Gly 
    130             


<210>  9
<211>  139
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN with a hexahistidine tag at the C-terminus

<400>  9

Met Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
1               5                   10                  15      


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
            20                  25                  30          


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
        35                  40                  45              


Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu 
    50                  55                  60                  


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
65                  70                  75                  80  


Val Leu Ser Ile Val Asn Arg Ala Gly Gly Gly Gly Lys Gly Gln Asp 
                85                  90                  95      


Asn Ser Ala Asp Ile Gln His Ser Gly Gly Arg Ser Ser Leu Glu Gly 
            100                 105                 110         


Pro Arg Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp 
        115                 120                 125             


Ser Thr Arg Thr Gly His His His His His His 
    130                 135                 


<210>  10
<211>  236
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MINFULL

<400>  10

Met Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
1               5                   10                  15      



Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
            20                  25                  30          


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
        35                  40                  45              


Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu 
    50                  55                  60                  


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
65                  70                  75                  80  


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
                85                  90                  95      


Phe Gln Thr His Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly 
            100                 105                 110         


Ile Glu Glu Glu Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu 
        115                 120                 125             


Val Asn Gly Ser Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys 
    130                 135                 140                 


Leu Phe Ser Tyr His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
145                 150                 155                 160 


Ile Val Glu Leu Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp 
                165                 170                 175     


Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val 
            180                 185                 190         


Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg 
        195                 200                 205             


Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro 
    210                 215                 220                 


Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu Leu 
225                 230                 235     


<210>  11
<211>  281
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MINFULL with a tetrad glycine linker and a V5 vector epitope
       tag at the C-terminus

<400>  11

Met Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
1               5                   10                  15      


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
            20                  25                  30          


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
        35                  40                  45              


Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu 
    50                  55                  60                  


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
65                  70                  75                  80  


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
                85                  90                  95      


Phe Gln Thr His Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly 
            100                 105                 110         


Ile Glu Glu Glu Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu 
        115                 120                 125             


Val Asn Gly Ser Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys 
    130                 135                 140                 


Leu Phe Ser Tyr His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
145                 150                 155                 160 


Ile Val Glu Leu Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp 
                165                 170                 175     


Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val 
            180                 185                 190         



Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg 
        195                 200                 205             


Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro 
    210                 215                 220                 


Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu Leu Gly Gly Gly Gly 
225                 230                 235                 240 


Lys Gly Gln Asp Asn Ser Ala Asp Ile Gln His Ser Gly Gly Arg Ser 
                245                 250                 255     


Ser Leu Glu Gly Pro Arg Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu 
            260                 265                 270         


Leu Gly Leu Asp Ser Thr Arg Thr Gly 
        275                 280     


<210>  12
<211>  287
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MINFULL with a hexahistidine tag at the C-terminus

<400>  12

Met Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asn 
1               5                   10                  15      


Asn Tyr Thr Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln 
            20                  25                  30          


Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
        35                  40                  45              


Leu Trp Asn Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu 
    50                  55                  60                  


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
65                  70                  75                  80  


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
                85                  90                  95      


Phe Gln Thr His Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly 
            100                 105                 110         


Ile Glu Glu Glu Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu 
        115                 120                 125             


Val Asn Gly Ser Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys 
    130                 135                 140                 


Leu Phe Ser Tyr His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
145                 150                 155                 160 


Ile Val Glu Leu Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp 
                165                 170                 175     


Trp Asn Leu Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val 
            180                 185                 190         


Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg 
        195                 200                 205             


Val Ile Glu Val Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro 
    210                 215                 220                 


Arg Arg Ile Arg Gln Gly Leu Glu Arg Ile Leu Leu Gly Gly Gly Gly 
225                 230                 235                 240 


Lys Gly Gln Asp Asn Ser Ala Asp Ile Gln His Ser Gly Gly Arg Ser 
                245                 250                 255     


Ser Leu Glu Gly Pro Arg Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu 
            260                 265                 270         


Leu Gly Leu Asp Ser Thr Arg Thr Gly His His His His His His 
        275                 280                 285         


<210>  13
<211>  116
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLE

<400>  13

Met Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Ile Trp Asn 
            20                  25                  30          


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
        35                  40                  45              


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
    50                  55                  60                  


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
65                  70                  75                  80  


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
                85                  90                  95      


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
            100                 105                 110         


Val Asn Arg Ala 
        115     


<210>  14
<211>  161
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN with a tetrad glycine linker and a V5 vector epitope tag
       at the C-terminus

<400>  14

Met Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Ile Trp Asn 
            20                  25                  30          


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
        35                  40                  45              


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
    50                  55                  60                  


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
65                  70                  75                  80  


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
                85                  90                  95      


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
            100                 105                 110         


Val Asn Arg Ala Gly Gly Gly Gly Lys Gly Gln Asp Asn Ser Ala Asp 
        115                 120                 125             


Ile Gln His Ser Gly Gly Arg Ser Ser Leu Glu Gly Pro Arg Phe Glu 
    130                 135                 140                 


Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Arg Thr 
145                 150                 155                 160 


Gly 
    


<210>  15
<211>  167
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLE with an additional hexahistidine tag at the 
       C-terminus

<400>  15

Met Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Ile Trp Asn 
            20                  25                  30          


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
        35                  40                  45              


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
    50                  55                  60                  


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
65                  70                  75                  80  


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
                85                  90                  95      


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
            100                 105                 110         


Val Asn Arg Ala Gly Gly Gly Gly Lys Gly Gln Asp Asn Ser Ala Asp 
        115                 120                 125             


Ile Gln His Ser Gly Gly Arg Ser Ser Leu Glu Gly Pro Arg Phe Glu 
    130                 135                 140                 


Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Arg Thr 
145                 150                 155                 160 


Gly His His His His His His 
                165         


<210>  16
<211>  264
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLEFULL

<400>  16

Met Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Ile Trp Asn 
            20                  25                  30          


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
        35                  40                  45              


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
    50                  55                  60                  


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
65                  70                  75                  80  


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
                85                  90                  95      


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
            100                 105                 110         


Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His 
        115                 120                 125             


Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu 
    130                 135                 140                 


Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser 
145                 150                 155                 160 


Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr 
                165                 170                 175     


His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu 
            180                 185                 190         


Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu 
        195                 200                 205             


Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn 
    210                 215                 220                 


Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val 
225                 230                 235                 240 


Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 
                245                 250                 255     


Gln Gly Leu Glu Arg Ile Leu Leu 
            260                 


<210>  17
<211>  309
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLEFULL with a tetrad glycine linker and a V5 vector 
       epitope tag at the C-terminus

<400>  17


Met Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Ile Trp Asn 
            20                  25                  30          



His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
        35                  40                  45              


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
    50                  55                  60                  


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
65                  70                  75                  80  


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
                85                  90                  95      


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
            100                 105                 110         


Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His 
        115                 120                 125             


Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu 
    130                 135                 140                 


Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser 
145                 150                 155                 160 


Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr 
                165                 170                 175     


His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu 
            180                 185                 190         


Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu 
        195                 200                 205             


Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn 
    210                 215                 220                 



Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val 
225                 230                 235                 240 


Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 
                245                 250                 255     


Gln Gly Leu Glu Arg Ile Leu Leu Gly Gly Gly Gly Lys Gly Gln Asp 
            260                 265                 270         


Asn Ser Ala Asp Ile Gln His Ser Gly Gly Arg Ser Ser Leu Glu Gly 
        275                 280                 285             


Pro Arg Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp 
    290                 295                 300                 


Ser Thr Arg Thr Gly 
305                 


<210>  18
<211>  315
<212>  PRT
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLEFULL with a hexahistidine tag at the C-terminus

<400>  18

Met Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala 
1               5                   10                  15      


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Ile Trp Asn 
            20                  25                  30          


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
        35                  40                  45              


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
    50                  55                  60                  


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
65                  70                  75                  80  


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
                85                  90                  95      


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
            100                 105                 110         


Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His 
        115                 120                 125             


Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu 
    130                 135                 140                 


Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser 
145                 150                 155                 160 


Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr 
                165                 170                 175     


His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu 
            180                 185                 190         


Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu 
        195                 200                 205             


Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn 
    210                 215                 220                 


Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val 
225                 230                 235                 240 


Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 
                245                 250                 255     


Gln Gly Leu Glu Arg Ile Leu Leu Gly Gly Gly Gly Lys Gly Gln Asp 
            260                 265                 270         


Asn Ser Ala Asp Ile Gln His Ser Gly Gly Arg Ser Ser Leu Glu Gly 
        275                 280                 285             


Pro Arg Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp 
    290                 295                 300                 


Ser Thr Arg Thr Gly His His His His His His 
305                 310                 315 


<210>  19
<211>  276
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN

<400>  19
atgatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc       60

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta      120

ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg      180

tacataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct      240

gtactttcta tagtgaatag agctggtggc ggtggc                                276


<210>  20
<211>  399
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN with a tetrad glycine linker and a V5 vector epitope tag

<400>  20
atgatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc       60

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta      120

ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg      180

tacataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct      240

gtactttcta tagtgaatag agctggtggc ggtggcaagg gtcaagacaa ttctgcagat      300

atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc      360

cctaaccctc tcctcggtct cgattctacg cgtaccggt                             399


<210>  21
<211>  417
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MIN with a hexahistidine tag

<400>  21
atgatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc       60

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta      120

ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg      180

tacataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct      240

gtactttcta tagtgaatag agctggtggc ggtggcaagg gtcaagacaa ttctgcagat      300

atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc      360

cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccat         417


<210>  22
<211>  708
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MINFULL

<400>  22
atgatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc       60

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta      120

ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg      180

tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct      240

gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac      300

ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga      360

gacagagaca gatccattcg attagtgaac ggatccttgg cacttatctg ggacgatctg      420

cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg      480

attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta      540

cagtattgga gtcaggaact aaagaatagt gctgttagct tgctcaatgc cacagccata      600

gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt      660

cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgcta                   708


<210>  23
<211>  843
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MINFULL with a tetrad glycine linker and a V5 vector epitope
       tag

<400>  23
atgatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc       60

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta      120

ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg      180

tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct      240

gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac      300

ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga      360

gacagagaca gatccattcg attagtgaac ggatccttgg cacttatctg ggacgatctg      420

cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg      480

attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta      540

cagtattgga gtcaggaact aaagaatagt gctgttagct tgctcaatgc cacagccata      600

gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt      660

cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgctagg tggcggtggc      720

aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc      780

ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc      840

ggt                                                                    843


<210>  24
<211>  861
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-MINFULL with a hexahistidine tag

<400>  24
atgatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc       60

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta      120

ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg      180

tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct      240

gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac      300

ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga      360

gacagagaca gatccattcg attagtgaac ggatccttgg cacttatctg ggacgatctg      420

cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg      480

attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta      540

cagtattgga gtcaggaact aaagaatagt gctgttagct tgctcaatgc cacagccata      600

gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt      660

cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgctagg tggcggtggc      720

aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc      780

ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc      840

ggtcatcatc accatcacca t                                                861


<210>  25
<211>  360
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLE

<400>  25
atggcagtgg gaataggagc tatgttcctt gggttcttgg gagcagcagg aagcactatg       60

ggcgcagcct caatgacgct gacggtaatt tggaatcaca cgacctggat ggagtgggac      120

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag      180

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg      240

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg      300

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagctgg tggcggtggc      360


<210>  26
<211>  483
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLE with a tetrad glycine linker and a V5 vector epitope 
       tag

<400>  26
atggcagtgg gaataggagc tatgttcctt gggttcttgg gagcagcagg aagcactatg       60

ggcgcagcct caatgacgct gacggtaatt tggaatcaca cgacctggat ggagtgggac      120

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag      180

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg      240

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg      300

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagctgg tggcggtggc      360

aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc      420

ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc      480

ggt                                                                    483


<210>  27
<211>  501
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLE with a hexahistidine tag

<400>  27
atggcagtgg gaataggagc tatgttcctt gggttcttgg gagcagcagg aagcactatg       60

ggcgcagcct caatgacgct gacggtaatt tggaatcaca cgacctggat ggagtgggac      120

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag      180

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg      240

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg      300

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagctgg tggcggtggc      360

aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc      420

ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc      480

ggtcatcatc accatcacca t                                                501


<210>  28
<211>  792
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLEFULL

<400>  28
atggcagtgg gaataggagc tatgttcctt gggttcttgg gagcagcagg aagcactatg       60

ggcgcagcct caatgacgct gacggtaatt tggaatcaca cgacctggat ggagtgggac      120

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag      180

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg      240

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg      300

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat      360

tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga      420

atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcc      480

ttggcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca ccgcttgaga      540

gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg gtgggaagcc      600

ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa tagtgctgtt      660

agcttgctca atgccacagc catagcagta gctgagggga cagatagggt tatagaagta      720

gtacaaggag cttgtagagc tattcgccac atacctagaa gaataagaca gggcttggaa      780

aggattttgc ta                                                          792


<210>  29
<211>  927
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLEFULL with a tetrad glycine linker and a V5 vector 
       epitope tag

<400>  29
atggcagtgg gaataggagc tatgttcctt gggttcttgg gagcagcagg aagcactatg       60

ggcgcagcct caatgacgct gacggtaatt tggaatcaca cgacctggat ggagtgggac      120

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag      180

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg      240

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg      300

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat      360

tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga      420

atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcc      480

ttggcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca ccgcttgaga      540

gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg gtgggaagcc      600

ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa tagtgctgtt      660

agcttgctca atgccacagc catagcagta gctgagggga cagatagggt tatagaagta      720

gtacaaggag cttgtagagc tattcgccac atacctagaa gaataagaca gggcttggaa      780

aggattttgc taggtggcgg tggcaagggt caagacaatt ctgcagatat ccagcacagt      840

ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc      900

ctcggtctcg attctacgcg taccggt                                          927


<210>  30
<211>  945
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GP41-STAPLEFULL with a hexahistidine tag

<400>  30
atggcagtgg gaataggagc tatgttcctt gggttcttgg gagcagcagg aagcactatg       60

ggcgcagcct caatgacgct gacggtaatt tggaatcaca cgacctggat ggagtgggac      120

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag      180

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg      240

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg      300

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat      360

tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga      420

atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcc      480

ttggcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca ccgcttgaga      540

gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg gtgggaagcc      600

ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa tagtgctgtt      660

agcttgctca atgccacagc catagcagta gctgagggga cagatagggt tatagaagta      720

gtacaaggag cttgtagagc tattcgccac atacctagaa gaataagaca gggcttggaa      780

aggattttgc taggtggcgg tggcaagggt caagacaatt ctgcagatat ccagcacagt      840

ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc      900

ctcggtctcg attctacgcg taccggtcat catcaccatc accat                      945


<210>  31
<211>  28
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  primer GP41-EC-f forward amplifying the fusion peptide

<400>  31
caccatggca gtgggaatag gagctatg                                          28


<210>  32
<211>  23
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  Primer FP reverse amplifying the fusion peptide

<400>  32
taccgtcagc gtcattgagg ctg                                               23


<210>  33
<211>  29
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  Primer GP41-2L forward amplifying the HR1 region

<400>  33
caccatgcag gccagacaat tattgtctg                                         29


<210>  34
<211>  28
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  Primer GP41-MAX forward amplifying the HR2 region

<400>  34
caccatgatt tggaataaca tgacctgg                                          28


<210>  35
<211>  31
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  Primer HR-2 f-2 forward amplifying the HR2 region

<400>  35
gctgacggta atttggaatc acacgacctg g                                      31


<210>  36
<211>  31
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  Primer GP41 reverse amplifying the transmembrane region

<400>  36
gccaccgcca cctagctcta ttcactatag a                                      31


<210>  37
<211>  29
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  Primer GP41-MAX reverse amplifying the Intracellular region

<400>  37
gccaccgcca cctagcaaaa tcctttcca                                         29






