                         SEQUENCE LISTING

<110>  Consejo Superior de Investigaciones Cientificas
 
<120>  NUCLEIC ACIDS ENCODING PRRSV GP5-ECTO AND M PROTEIN

<130>  P86098

<160>  11    

<170>  PatentIn version 3.3

<210>  1
<211>  68
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  Gp5 ecto domain sequence of PRRSV strain Olot91.

<400>  1

Met Arg Cys Ser His Lys Leu Gly Arg Phe Leu Thr Pro His Ser Cys 
1               5                   10                  15      


Phe Trp Trp Leu Phe Leu Leu Cys Thr Gly Leu Ser Trp Ser Phe Val 
            20                  25                  30          


Ala Gly Ser Gly Ser Ser Ser Thr Tyr Gln Tyr Ile Tyr Asn Leu Thr 
        35                  40                  45              


Ile Cys Glu Leu Asn Gly Thr Asp Trp Leu Ser Asn His Phe Asp Trp 
    50                  55                  60                  


Ala Val Glu Thr 
65              


<210>  2
<211>  173
<212>  PRT
<213>  porcine reproductive and respiratory syndrome virus 


<220>
<221>  misc_feature
<223>  M protein sequence of PRRSV strain Olot91.

<400>  2

Met Gly Ser Leu Asp Asp Phe Cys Asn Asp Ser Thr Ala Ala Gln Lys 
1               5                   10                  15      


Leu Val Leu Ala Phe Ser Ile Thr Tyr Thr Pro Ile Met Ile Tyr Ala 
            20                  25                  30          


Leu Lys Val Ser Arg Gly Arg Leu Leu Gly Leu Leu His Ile Leu Ile 
        35                  40                  45              


Phe Leu Asn Cys Ser Phe Thr Phe Gly Tyr Met Thr Tyr Val Arg Phe 
    50                  55                  60                  


Gln Ser Thr Asn Arg Val Ala Leu Thr Leu Gly Ala Val Val Ala Leu 
65                  70                  75                  80  


Leu Trp Gly Val Tyr Ser Phe Thr Glu Ser Trp Lys Phe Val Thr Ser 
                85                  90                  95      


Arg Cys Arg Leu Cys Cys Leu Gly Arg Arg Tyr Ile Leu Ala Pro Ala 
            100                 105                 110         


His His Val Glu Ser Ala Ala Gly Leu His Ser Ile Pro Ala Ser Gly 
        115                 120                 125             


Asn Arg Ala Tyr Ala Val Arg Lys Pro Gly Leu Thr Ser Val Asn Gly 
    130                 135                 140                 


Thr Leu Val Pro Gly Leu Arg Ser Leu Val Leu Gly Gly Lys Arg Ala 
145                 150                 155                 160 


Val Lys Arg Gly Val Val Asn Leu Val Lys Tyr Gly Arg 
                165                 170             


<210>  3
<211>  133
<212>  PRT
<213>  porcine reproductive and respiratory syndrome virus 


<220>
<221>  misc_feature
<223>  Gp5 protein sequence lacking the ecto domain sequence of PRRSV strain Olot91.

<400>  3

Phe Val Leu Tyr Pro Val Ala Thr His Ile Leu Ser Leu Gly Phe Leu 
1               5                   10                  15      


Thr Thr Ser His Phe Phe Asp Ala Leu Gly Leu Gly Ala Val Ser Thr 
            20                  25                  30          


Thr Gly Phe Val Gly Gly Arg Tyr Val Leu Ser Ser Val Tyr Gly Ala 
        35                  40                  45              


Cys Ala Phe Ala Ala Leu Val Cys Phe Val Ile Arg Ala Val Lys Asn 
    50                  55                  60                  


Cys Met Ala Cys Arg Tyr Ala His Thr Arg Phe Thr Asn Phe Ile Val 
65                  70                  75                  80  


Asp Asp Arg Gly Arg Ile His Arg Trp Lys Ser Pro Ile Val Val Glu 
                85                  90                  95      


Lys Leu Gly Lys Ala Glu Val Gly Gly Asp Leu Val Thr Ile Lys His 
            100                 105                 110         


Val Val Leu Glu Gly Val Lys Ala Gln Pro Leu Thr Arg Thr Ser Ala 
        115                 120                 125             


Glu Gln Trp Glu Ala 
    130             


<210>  4
<211>  66
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  Gp5 ecto domain sequence of PRRSV strain VR2332.

<400>  4

Met Leu Glu Lys Cys Leu Thr Ala Gly Cys Cys Ser Arg Leu Leu Ser 
1               5                   10                  15      


Leu Trp Cys Ile Val Pro Phe Cys Phe Ala Val Leu Ala Asn Ala Ser 
            20                  25                  30          


Asn Asp Ser Ser Ser His Leu Gln Leu Ile Tyr Asn Leu Thr Leu Cys 
        35                  40                  45              


Glu Leu Asn Gly Thr Asp Trp Leu Ala Asn Lys Phe Asp Trp Ala Val 
    50                  55                  60                  


Glu Ser 
65      


<210>  5
<211>  174
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  M protein sequence of PRRSV strain VR2332.

<400>  5

Met Gly Ser Ser Leu Asp Asp Phe Cys His Asp Ser Thr Ala Pro Gln 
1               5                   10                  15      


Lys Val Leu Leu Ala Phe Ser Ile Thr Tyr Thr Pro Val Met Ile Tyr 
            20                  25                  30          


Ala Leu Lys Val Ser Arg Gly Arg Leu Leu Gly Leu Leu His Leu Leu 
        35                  40                  45              


Ile Phe Leu Asn Cys Ala Phe Thr Phe Gly Tyr Met Thr Phe Ala His 
    50                  55                  60                  


Phe Gln Ser Thr Asn Lys Val Ala Leu Thr Met Gly Ala Val Val Ala 
65                  70                  75                  80  


Leu Leu Trp Gly Val Tyr Ser Ala Ile Glu Thr Trp Lys Phe Ile Thr 
                85                  90                  95      


Ser Arg Cys Arg Leu Cys Leu Leu Gly Arg Lys Tyr Ile Leu Ala Pro 
            100                 105                 110         


Ala His His Val Glu Ser Ala Ala Arg Phe His Pro Ile Ala Ala Asn 
        115                 120                 125             


Asp Asn His Ala Phe Val Val Arg Arg Pro Gly Ser Thr Thr Val Asn 
    130                 135                 140                 


Gly Thr Leu Val Pro Gly Leu Lys Ser Leu Val Leu Gly Gly Arg Lys 
145                 150                 155                 160 


Ala Val Lys Gln Gly Val Val Asn Leu Val Lys Tyr Ala Lys 
                165                 170                 


<210>  6
<211>  134
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  Gp5 protein sequence lacking the ecto domain sequence of PRRSV strain VR2332.

<400>  6

Phe Val Ile Phe Pro Val Leu Thr His Ile Val Ser Tyr Gly Ala Leu 
1               5                   10                  15      


Thr Thr Ser His Phe Leu Asp Thr Val Ala Leu Val Thr Val Ser Thr 
            20                  25                  30          


Ala Gly Phe Val His Gly Arg Tyr Val Leu Ser Ser Ile Tyr Ala Val 
        35                  40                  45              


Cys Ala Leu Ala Ala Leu Thr Cys Phe Val Ile Arg Phe Ala Lys Asn 
    50                  55                  60                  


Cys Met Ser Trp Arg Tyr Ala Cys Thr Arg Tyr Thr Asn Phe Leu Leu 
65                  70                  75                  80  


Asp Thr Lys Gly Arg Leu Tyr Arg Trp Arg Ser Pro Val Ile Ile Glu 
                85                  90                  95      


Lys Arg Gly Lys Val Glu Val Glu Gly His Leu Ile Asp Leu Lys Arg 
            100                 105                 110         


Val Val Leu Asp Gly Ser Val Ala Thr Pro Ile Thr Arg Val Ser Ala 
        115                 120                 125             


Glu Gln Trp Gly Arg Pro 
    130                 


<210>  7
<211>  66
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  Gp5 ecto domain sequence of  PRRSV strain HB-2(sh).

<400>  7

Met Leu Gly Lys Cys Leu Thr Ala Gly Cys Tyr Ser Gln Leu Leu Ser 
1               5                   10                  15      


Leu Trp Cys Ile Val Pro Phe Cys Phe Ala Val Leu Val Asn Ala Ser 
            20                  25                  30          


Ser Asn Ser Ser Pro His Phe Gln Leu Ile Tyr Asn Leu Thr Leu Cys 
        35                  40                  45              


Glu Leu Asn Gly Thr Asp Trp Leu Ala Glu Arg Phe Asp Trp Ala Val 
    50                  55                  60                  


Glu Thr 
65      


<210>  8
<211>  174
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  M protein sequence of  PRRSV strain HB-2(sh).

<400>  8

Met Gly Ser Ser Leu Asp Asp Phe Cys His Asp Ser Thr Ala Pro Gln 
1               5                   10                  15      


Lys Val Leu Leu Ala Phe Ser Ile Thr Tyr Thr Pro Val Met Ile Tyr 
            20                  25                  30          


Ala Leu Lys Val Ser Arg Gly Arg Leu Leu Gly Leu Leu His Leu Leu 
        35                  40                  45              


Ile Phe Leu Asn Cys Ala Phe Thr Phe Gly Tyr Met Thr Phe Val His 
    50                  55                  60                  


Phe Gln Ser Thr Asn Arg Val Ala Leu Thr Met Gly Ala Val Val Ala 
65                  70                  75                  80  


Leu Leu Trp Gly Val Tyr Ser Ala Ile Glu Thr Trp Lys Phe Ile Thr 
                85                  90                  95      


Ser Arg Cys Arg Leu Cys Leu Leu Gly Arg Lys Tyr Ile Leu Ala Pro 
            100                 105                 110         


Ala His His Val Glu Ser Ala Ala Gly Phe His Pro Ile Ala Ala Asn 
        115                 120                 125             


Asp Asn His Ala Phe Val Val Arg Arg Pro Gly Ser Thr Thr Val Asn 
    130                 135                 140                 


Gly Ile Leu Val Pro Gly Leu Lys Ser Leu Val Leu Gly Gly Arg Lys 
145                 150                 155                 160 


Ala Val Lys Gln Gly Val Val Asn Leu Val Lys Tyr Ala Lys 
                165                 170                 


<210>  9
<211>  134
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  Gp5 protein sequence lacking the ecto domain sequence of PRRSV strain HB-2(sh).

<400>  9

Phe Val Ile Phe Pro Val Leu Thr His Ile Val Ser Tyr Gly Ala Leu 
1               5                   10                  15      


Thr Thr Ser His Phe Leu Asp Thr Val Gly Leu Ile Thr Val Ser Thr 
            20                  25                  30          


Ala Gly Phe Tyr His Arg Arg Tyr Val Leu Ser Ser Ile Tyr Ala Val 
        35                  40                  45              


Cys Ala Leu Ala Ala Leu Ile Cys Phe Val Ile Arg Leu Ala Lys Asn 
    50                  55                  60                  


Cys Met Ser Trp Arg Tyr Ser Cys Thr Arg Tyr Thr Asn Phe Leu Leu 
65                  70                  75                  80  


Asp Thr Lys Gly Arg Leu Tyr Arg Trp Arg Pro Pro Val Ile Val Glu 
                85                  90                  95      


Lys Gly Gly Lys Val Glu Val Glu Gly His Leu Ile Asp Leu Lys Arg 
            100                 105                 110         


Val Val Leu Asp Gly Ser Val Ala Thr Pro Leu Thr Arg Val Ser Ala 
        115                 120                 125             


Glu Gln Trp Gly Arg Leu 
    130                 


<210>  10
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  flexible linker sequence

<400>  10

Gly Gly Pro Gly Gly 
1               5   


<210>  11
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Epitope tag

<400>  11

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  12
<211>  63
<212>  PRT
<213>  Porcine reproductive and respiratory syndrome virus


<220>
<221>  misc_feature
<223>  Gp5 ecto domain sequence of European PRRSV strain Olot91 with 
       N46S mutation and deletion of amino acids 30-34

<400>  12

Met Arg Cys Ser His Lys Leu Gly Arg Phe Leu Thr Pro His Ser Cys 
1               5                   10                  15      


Phe Trp Trp Leu Phe Leu Leu Cys Thr Gly Leu Ser Trp Ser Gly Ser 
            20                  25                  30          


Ser Ser Thr Tyr Gln Tyr Ile Tyr Ser Leu Thr Ile Cys Glu Leu Asn 
        35                  40                  45              


Gly Thr Asp Trp Leu Ser Asn His Phe Asp Trp Ala Val Glu Thr 
    50                  55                  60              






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