                               SEQUENCE LISTING                               

<110> Transposagen Biopharmaceuticals, Inc.
      Ostertag, Eric
      Madison, Blair

<120> Hyperactive PiggyBac Transposases

<130> 128578.00402

<140> PCT/US2011/061054
<141> 2011-11-16

<150> 61/414,399
<151> 2010-11-16

<160> 108 

<170> PatentIn version 3.5

<210> 1
<211> 1782
<212> DNA
<213> Trichoplusia ni 

<400> 1
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 2
<211> 594
<212> PRT
<213> Trichoplusia ni 

<400> 2

Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 3
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223>  A46S PiggyBac


<400> 3
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa tcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ser Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240


ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480


agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 4
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> A46S PiggyBac 

<400> 4
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ser Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 5
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> A46T PiggyBac 

<400> 5
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa acc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Thr Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560


tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575


tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 6
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> A46T PiggyBac 

<400> 6
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Thr Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 7
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> R119P PiggyBac 

<400> 7
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag ccc ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Pro Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 8
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> R119P PiggyBac 

<400> 8
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Pro Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 9
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> C125A PiggyBac

<400> 9
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg gcc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Ala Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495


cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 10
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> C125A PiggyBac 

<400> 10
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Ala Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 11
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> C125L PiggyBac

<400> 11
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg ctg aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Leu Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590


tgt ttc                                                            1782
Cys Phe


<210> 12
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> C125L PiggyBac

<400> 12
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Leu Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 13
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> Y177K PiggyBac

<400> 13
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160


gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

aag gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Lys Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 14
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> Y177K PiggyBac

<400> 14
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Lys Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 15
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> Y177H PiggyBac

<400> 15
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

cac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
His Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 16
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> Y177H PiggyBac 

<400> 16
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

His Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 17
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> F180L PiggyBac 

<400> 17
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ctg ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Leu Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 18
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> F180L PiggyBac 

<400> 18
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Leu Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 19
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> F180I PiggyBac 


<400> 19
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc atc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Ile Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 20
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> F180I PiggyBac 


<400> 20
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Ile Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 21
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> F180V PiggyBac

<400> 21
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc gtg ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Val Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 22
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> F180V PiggyBac

<400> 22
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Val Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 23
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M185L PiggyBac 

<400> 23
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg ctg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Leu Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 24
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M185L PiggyBac 

<400> 24
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Leu Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 25
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> A187G PiggyBac

<400> 25
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc ggc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Gly Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 26
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> A187G PiggyBac

<400> 26
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Gly Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 27
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> F200W PiggyBac 

<400> 27
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg tgg gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Trp Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320


ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 28
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> F200W PiggyBac

<400> 28
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Trp Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 29
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> V207P PiggyBac

<400> 29
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg ccc tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Pro Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 30
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> V207P PiggyBac

<400> 30
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Pro Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 31
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  V209F PiggyBac

<400> 31
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

ttc tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Phe Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480


agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 32
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> V209F PiggyBac

<400> 32
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Phe Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 33
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  M226F PiggyBac 

<400> 33
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160


gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga ttc gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Phe Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560


tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 34
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M226F PiggyBac

<400> 34
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Phe Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540


Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 35
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  L235R PiggyBac

<400> 35
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160



gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc aga cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Arg Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400


ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 36
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> L235R PiggyBac

<400> 36
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Arg Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335


His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 37
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223>  V240K PiggyBac

<400> 37
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac aag     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Lys 
225                 230                 235                 240


ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 38
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> V240K PiggyBac 

<400> 38
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Lys 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 39
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> F241L PiggyBac 

<400> 39
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ctg acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Leu Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560


tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 40
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> F241L PiggyBac 


<400> 40
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Leu Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 41
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> P243K PiggyBac

<400> 41
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160


gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc aag gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Lys Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 42
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> P243K PiggyBac

<400> 42
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Lys Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 43
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M282Q PiggyBac

<400> 43
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga cag tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Gln Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 44
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M282Q PiggyBac 

<400> 44
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Gln Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 45
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> L296W PiggyBac

<400> 45
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc tgg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Trp Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 46
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> L296W PiggyBac

<400> 46
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Trp Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 47
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> L296Y PiggyBac

<400> 47
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc tac atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Tyr Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 48
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> L296Y PiggyBac 

<400> 48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Tyr Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 49
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> L296F PiggyBac 

<400> 49
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ttc atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Phe Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 50
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> L296F PiggyBac

<400> 50
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Phe Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 51
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M298L PiggyBac

<400> 51
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg ctg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Leu Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320


ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 52
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M298L PiggyBac

<400> 52
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Leu Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 53
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M298A PiggyBac

<400> 53
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg gcc tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Ala Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400


ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 54
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M298A PiggyBac 

<400> 54
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Ala Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 55
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M298V PiggyBac

<400> 55
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg gtg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Val Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 56
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M298V PiggyBac


<400> 56
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Val Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 57
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> P311I PiggyBac

<400> 57
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg atc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Ile Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 58
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> P311I PiggyBac

<400> 58
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Ile Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 59
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> P311V PiggyBac 

<400> 59
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg gtg tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Val Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 60
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> P311V PiggyBac

<400> 60
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Val Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 61
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> R315K PiggyBac

<400> 61
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240


ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aag ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Lys Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480


agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 62
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> R315K PiggyBac 

<400> 62
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Lys Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 63
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> T319G PiggyBac

<400> 63
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag ggc aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Gly Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 64
<211> 594
<212> PRT
<213>  Artificial Sequence

<220>
<223>  T319G PiggyBac

<400> 64
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Gly Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 65
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  Y327R PiggyBac

<400> 65
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160


gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag aga tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Arg Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400


ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 66
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> Y327R PiggyBac

<400> 66
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Arg Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335


His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 67
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> Y328V PiggyBac

<400> 67
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac gtg gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Val Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480


agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 68
<211> 594
<212> PRT
<213>  Artificial Sequence

<220>
<223>  Y328V PiggyBac

<400> 68
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Val Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 69
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  C340G PiggyBac

<400> 69
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc ggc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Gly Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 70
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> C340G PiggyBac

<400> 70
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Gly Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 71
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> C340L PiggyBac

<400> 71
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc ctg agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Leu Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 72
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> C340L PiggyBac

<400> 72
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Leu Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 73
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223>  D421H PiggyBac

<400> 73
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc cat gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys His Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 74
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> D421H PiggyBac

<400> 74
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys His Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 75
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  V436I PiggyBac

<400> 75
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg atc atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Ile Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 76
<211> 594
<212> PRT
<213>  Artificial Sequence

<220>
<223>  V436I PiggyBac

<400> 76
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 77
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M456Y PiggyBac

<400> 77
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg tac acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Tyr Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 78
<211> 594
<212> PRT
<213>  Artificial Sequence

<220>
<223>  M456Y PiggyBac

<400> 78
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Tyr Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 79
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> L470F PiggyBac

<400> 79
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ttc tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Phe Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480


agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 80
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> L470F PiggyBac 

<400> 80
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Phe Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 81
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> S486K PiggyBac

<400> 81
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac aag cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Lys His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 82
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> S486K PiggyBac

<400> 82
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Lys His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 83
<211> 1782
<212> DNA
<213>  Artificial Sequence

<220>
<223>  M503L PiggyBac

<400> 83
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc ctg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Leu Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 84
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M503L PiggyBac

<400> 84
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Leu Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 85
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> M503I PiggyBac

<400> 85
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atc cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Ile Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 86
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> M503I PiggyBac

<400> 86
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Ile Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 87
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> V552K PiggyBac

<400> 87
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc aag atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Lys Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 88
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> V552K PiggyBac

<400> 88
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Lys Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 89
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> A570T PiggyBac

<400> 89
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag acc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Thr Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 90
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> A570T PiggyBac

<400> 90
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Thr Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe

<210> 91
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> PiggyBac 5' inverted repeat

<400> 91
ccctagaaag atagtctgcg taaaattgac gcatg                35

<210> 92
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> PiggyBac 3' inverted repeat

<400> 92
ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc   60

atg                                                                 63

<210> 93
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> S3N PiggyBac

<400> 93
atg ggc aac agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Asn Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 94
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> S3N PiggyBac

<400> 94
Met Gly Asn Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe

<210> 95
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> I30V PiggyBac

<400> 95 
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag gtg agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe

<210> 96
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> I30V PiggyBac

<400> 96
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 97
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> I82W PiggyBac

<400> 97
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga tgg ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Trp Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 98
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> I82W PiggyBac

<400> 98
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Trp Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 99
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> S103P PiggyBac

<400> 99
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag cct acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 100
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> S103P PiggyBac

<400> 100
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 101
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> G165S PiggyBac

<400> 101
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc agc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 102
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> G165S PiggyBac

<400> 102
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 103
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> N258S PiggyBac

<400> 103
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag agc tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Ser Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cag agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 104
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> N258S PiggyBac

<400> 104
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Ser Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
            580                 585                 590

Cys Phe


<210> 105
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> Q591P PiggyBac

<400> 105
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc ccc agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Pro Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 106
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> Q591P PiggyBac

<400> 106
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Pro Ser
            580                 585                 590

Cys Phe



<210> 107
<211> 1782
<212> DNA
<213> Artificial Sequence

<220>
<223> Q591R PiggyBac

<400> 107
atg ggc tct agc ctg gac gac gag cac atc ctg agc gcc ctg ctg cag      48
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

agc gac gac gaa ctg gtg ggc gag gac agc gac agc gag atc agc gac      96
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

cac gtg tcc gag gac gac gtg cag tcc gac acc gag gaa gcc ttc atc     144
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

gac gag gtg cac gaa gtg cag cct acc agc agc ggc tcc gag atc ctg     192
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

gac gag cag aac gtg atc gag cag cct ggc agc tcc ctg gcc agc aac     240
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

aga atc ctg acc ctg ccc cag aga acc atc aga ggc aag aac aag cac     288
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

tgc tgg tcc acc tcc aag agc acc agg cgg agc aga gtg tcc gcc ctg     336
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

aac atc gtg cgg agc cag agg ggc ccc acc aga atg tgc aga aac atc     384
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

tac gac ccc ctg ctg tgc ttc aag ctg ttc ttc acc gac gag atc atc     432
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

agc gag atc gtg aag tgg acc aac gcc gag atc agc ctg aag agg cgg     480
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

gag agc atg acc ggc gcc acc ttc aga gac acc aac gag gac gag atc     528
Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

tac gcc ttc ttc ggc atc ctg gtg atg acc gcc gtg aga aag gac aac     576
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

cac atg agc acc gac gac ctg ttc gac aga tcc ctg agc atg gtg tac     624
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

gtg tcc gtg atg agc aga gac aga ttc gac ttc ctg atc aga tgc ctg     672
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

aga atg gac gac aag agc atc aga ccc acc ctg cgg gag aac gac gtg     720
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

ttc acc ccc gtg cgg aag atc tgg gac ctg ttc atc cac cag tgc atc     768
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

cag aac tac acc cct ggc gcc cac ctg acc atc gat gag cag ctg ctg     816
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

ggc ttc aga ggc aga tgc ccc ttc aga atg tac atc ccc aac aag ccc     864
Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

agc aag tac ggc atc aag atc ctg atg atg tgc gac agc ggc acc aag     912
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

tac atg atc aac ggc atg ccc tac ctg ggc aga ggc acc cag aca aac     960
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

ggc gtg ccc ctg ggc gag tac tac gtg aaa gaa ctg agc aag cct gtg    1008
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

cat ggc agc tgc agg aac atc acc tgc gac aac tgg ttc acc agc atc    1056
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

ccc ctg gcc aag aac ctg ctg cag gaa ccc tac aag ctg acc atc gtg    1104
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

ggc acc gtg cgg agc aac aag cgg gag atc cca gag gtg ctg aag aac    1152
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

agc aga tcc aga cct gtg gga aca agc atg ttc tgc ttc gac ggc ccc    1200
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

ctg acc ctg gtg tcc tac aag ccc aag ccc gcc aag atg gtg tac ctg    1248
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

ctg tcc agc tgc gac gag gac gcc agc atc aac gag agc acc ggc aag    1296
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

ccc cag atg gtg atg tac tac aac cag acc aag ggc ggc gtg gac acc    1344
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

ctg gac cag atg tgc agc gtg atg acc tgc agc aga aag acc aac aga    1392
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

tgg ccc atg gcc ctg ctg tac ggc atg atc aat atc gcc tgc atc aac    1440
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

agc ttc atc atc tac agc cac aac gtg tcc agc aag ggc gag aag gtg    1488
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

cag agc cgg aag aaa ttc atg cgg aac ctg tac atg agc ctg acc tcc    1536
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

agc ttc atg aga aag aga ctg gaa gcc ccc acc ctg aag aga tac ctg    1584
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

cgg gac aac atc agc aac atc ctg ccc aac gaa gtg cca gga aca agc    1632
Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

gac gac agc acc gag gaa ccc gtg atg aag aag agg acc tac tgc acc    1680
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

tac tgt ccc agc aag atc aga aga aag gcc aac gcc agc tgc aag aaa    1728
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

tgc aaa aaa gtg atc tgc cgg gag cac aac atc gac atg tgc cgg agc    1776
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Arg Ser
            580                 585                 590

tgt ttc                                                            1782
Cys Phe


<210> 108
<211> 594
<212> PRT
<213> Artificial Sequence

<220>
<223> Q591R PiggyBac

<400> 108
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
                  5                  10                  15

Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
             20                  25                  30

His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
         35                  40                  45

Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
     50                  55                  60

Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80

Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                 85                  90                  95

Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110

Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125

Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140

Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145                 150                 155                 160

Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175

Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190

His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
        195                 200                 205

Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220

Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240

Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255

Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270

Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285

Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300

Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320

Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335

His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350

Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365

Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380

Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400

Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415

Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430

Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445

Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460

Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480

Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495

Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510

Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525

Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540

Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560

Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575

Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Arg Ser
            580                 585                 590

Cys Phe


























