SEQUENCE LISTING

<110>  Abbott Laboratories
 
<120>  ANTI-IL-12/IL-23 ANTIBODIES AND USES THEREOF

<130>  117813-48220

<140>  New Application
<141>  2012-01-06

<150>  61/460,780
<151>  2011-01-07

<150>  12/121,615
<151>  2008-05-15

<150>  10/884,830
<151>  2004-07-01

<150>  09/534,717
<151>  2000-03-24

<150>  60/126,603
<151>  1999-03-25

<160>  26     

<170>  PatentIn version 3.5

<210> 1                                                                        
<211> 115                                                                       
<212> PRT                                                                       
<213> Homo sapiens   

<220>
<221>  MISC_FEATURE
<222>  (1)..(115)
<223>  ABT-874 Heavy Chain Variable Region
                                                           
                                                                                
<400> 1
   
                                                                     
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg                 
  1               5                  10                  15                     
                                                                                
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr                 
             20                  25                  30                         
                                                                                
Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val                 
         35                  40                  45                             
                                                                                
Ala Phe Ile Arg Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val                 
     50                  55                  60                                 
                                                                                
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr                 
 65                  70                  75                  80                 
                                                                                
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys                 
                 85                  90                  95                     
                                                                                
Lys Thr His Gly Ser His Asp Asn Trp Gly Gln Gly Thr Met Val Thr                 
            100                 105                 110                         
                                                                                
Val Ser Ser                                                                     
        115                                  


<210> 2                                                                        
<211> 112                                                                       
<212> PRT                                                                       
<213> Homo sapiens                                                              
                      
<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  ABT-874 Light Chain Variable Region
                                                          
<400> 2
                                                                       
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ala Pro Gly Gln                 
  1               5                  10                  15                     
                                                                                
Arg Val Thr Ile Ser Cys Ser Gly Ser Arg Ser Asn Ile Gly Ser Asn                 
             20                  25                  30                         
                                                                                
Thr Val Lys Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu                 
         35                  40                  45                             
                                                                                
Ile Tyr Tyr Asn Asp Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser                 
     50                  55                  60                                 
                                                                                
Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln                 
 65                  70                  75                  80                 
                                                                                
Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Arg Tyr Thr                 
                 85                  90                  95                     
                                                                                
His Pro Ala Leu Leu Phe Gly Thr Gly Thr Lys Val Thr Val Leu Gly                 
            100                 105                 110             



<210>  3
<211>  306
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(306)
<223>  mature form, human IL-12 P40 subunit.  Swiss-Prot P29460

<400>  3

Ile Trp Glu Leu Lys Lys Asp Val Tyr Val Val Glu Leu Asp Trp Tyr 
1               5                   10                  15      


Pro Asp Ala Pro Gly Glu Met Val Val Leu Thr Cys Asp Thr Pro Glu 
            20                  25                  30          


Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln Ser Ser Glu Val Leu Gly 
        35                  40                  45              


Ser Gly Lys Thr Leu Thr Ile Gln Val Lys Glu Phe Gly Asp Ala Gly 
    50                  55                  60                  


Gln Tyr Thr Cys His Lys Gly Gly Glu Val Leu Ser His Ser Leu Leu 
65                  70                  75                  80  


Leu Leu His Lys Lys Glu Asp Gly Ile Trp Ser Thr Asp Ile Leu Lys 
                85                  90                  95      


Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe Leu Arg Cys Glu Ala Lys 
            100                 105                 110         


Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp Leu Thr Thr Ile Ser Thr 
        115                 120                 125             


Asp Leu Thr Phe Ser Val Lys Ser Ser Arg Gly Ser Ser Asp Pro Gln 
    130                 135                 140                 


Gly Val Thr Cys Gly Ala Ala Thr Leu Ser Ala Glu Arg Val Arg Gly 
145                 150                 155                 160 


Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu Cys Gln Glu Asp Ser Ala 
                165                 170                 175     


Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile Glu Val Met Val Asp Ala 
            180                 185                 190         


Val His Lys Leu Lys Tyr Glu Asn Tyr Thr Ser Ser Phe Phe Ile Arg 
        195                 200                 205             


Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn Leu Gln Leu Lys Pro Leu 
    210                 215                 220                 


Lys Asn Ser Arg Gln Val Glu Val Ser Trp Glu Tyr Pro Asp Thr Trp 
225                 230                 235                 240 


Ser Thr Pro His Ser Tyr Phe Ser Leu Thr Phe Cys Val Gln Val Gln 
                245                 250                 255     


Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg Val Phe Thr Asp Lys Thr 
            260                 265                 270         


Ser Ala Thr Val Ile Cys Arg Lys Asn Ala Ser Ile Ser Val Arg Ala 
        275                 280                 285             


Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser Glu Trp Ala Ser Val Pro 
    290                 295                 300                 


Cys Ser 
305     


<210>  4
<211>  197
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(197)
<223>  mature form, human IL-12 P35 subunit.  Swiss-Prot P29459

<400>  4

Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly Met Phe Pro Cys Leu 
1               5                   10                  15      


His His Ser Gln Asn Leu Leu Arg Ala Val Ser Asn Met Leu Gln Lys 
            20                  25                  30          


Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr Ser Glu Glu Ile Asp 
        35                  40                  45              


His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr Val Glu Ala Cys Leu 
    50                  55                  60                  


Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu Asn Ser Arg Glu Thr 
65                  70                  75                  80  


Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser Arg Lys Thr Ser Phe 
                85                  90                  95      


Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu Asp Leu Lys Met Tyr 
            100                 105                 110         


Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu Leu Met Asp Pro Lys 
        115                 120                 125             


Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala Val Ile Asp Glu Leu 
    130                 135                 140                 


Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val Pro Gln Lys Ser Ser 
145                 150                 155                 160 


Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile Lys Leu Cys Ile Leu 
                165                 170                 175     


Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile Asp Arg Val Met Ser 
            180                 185                 190         


Tyr Leu Asn Ala Ser 
        195         


<210> 5                                                                        
<211> 328                                                                       
<212> PRT                                                                       
<213> Homo sapiens   

<220>
<221>  MISC_FEATURE
<222>  (1)..(328)
<223>  Precursor p40 Peptide - sp|P29460|IL12B_HUMAN Interleukin-12 subunit beta OS=Homo sapiens GN=IL12B PE=1 SV=1
                                                           
                                                                                
<400> 5
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser 
                325


<210> 6                                                                        
<211> 219                                                                       
<212> PRT                                                                       
<213> Homo sapiens   

<220>
<221>  MISC_FEATURE
<222>  (1)..(219)
<223>  Precursor p35 Peptide - sp|P29459|IL12A_HUMAN Interleukin-12 subunit alpha OS=Homo sapiens GN=IL12A PE=1 SV=2
                                                           
                                                                                
<400> 6

Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val Leu Leu 
1               5                   10                  15      


Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro Asp Pro 
            20                  25                  30          


Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val 
        35                  40                  45              


Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys 
    50                  55                  60                  


Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser 
65                  70                  75                  80  


Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys 
                85                  90                  95      


Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala 
            100                 105                 110         


Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr 
        115                 120                 125             


Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys 
    130                 135                 140                 


Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu 
145                 150                 155                 160 


Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr 
                165                 170                 175     


Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys 
            180                 185                 190         


Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr 
        195                 200                 205             


Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    210                 215                 


<210>  7
<211>  25
<212>  DNA
<213>  Synthetic

<400>  7
caccatgggt caccagcagt tggtc                                             25


<210>  8
<211>  47
<212>  DNA
<213>  Synthetic

<400>  8
accctggaag tacaggtttt cactgcaggg cacagatgcc cattcgc                     47


<210>  9
<211>  35
<212>  DNA
<213>  Synthetic

<400>  9
cgtagaattg gattggcgtc cggatgcccc tggag                                  35


<210>  10
<211>  35
<212>  DNA
<213>  Synthetic

<400>  10
ctccaggggc atccggacgc caatccaatt ctacg                                  35


<210>  11
<211>  37
<212>  DNA
<213>  Synthetic

<400>  11
ctgcttcaca aaaaggaaaa cggaatttgg tccactg                                37


<210>  12
<211>  37
<212>  DNA
<213>  Synthetic

<400>  12
cagtggacca aattccgttt tcctttttgt gaagcag                                37


<210>  13
<211>  40
<212>  DNA
<213>  Synthetic

<400>  13
gatggaattt ggtccactga gattttaaag gaccagaaag                             40


<210>  14
<211>  40
<212>  DNA
<213>  Synthetic

<400>  14
ctttctggtc ctttaaaatc tcagtggacc aaattccatc                             40


<210>  15
<211>  58
<212>  DNA
<213>  Synthetic

<400>  15
ggtccactga tattttaaag aaccagaaag aattcaaaaa taagaccttt ctaagatg         58


<210>  16
<211>  58
<212>  DNA
<213>  Synthetic

<400>  16
catcttagaa aggtcttatt tttgaattct ttctggttct ttaaaatatc agtggacc         58


<210>  17
<211>  40
<212>  DNA
<213>  Synthetic

<400>  17
gacacccctg aagaagatga catcacctgg accttggacc                             40


<210>  18
<211>  40
<212>  DNA
<213>  Synthetic

<400>  18
ggtccaaggt ccaggtgatg tcatcttctt caggggtgtc                             40


<210>  19
<211>  60
<212>  DNA
<213>  Synthetic

<400>  19
gatggtatca cctggacctc cgaccagcgc cggggggtca tcggctctgg caaaaccctg       60


<210>  20
<211>  60
<212>  DNA
<213>  Synthetic

<400>  20
ggtcagggtt ttgccagagc cgatgacccc ccggcgctgg tcggaggtcc aggtgatacc       60


<210>  21
<211>  56
<212>  DNA
<213>  Synthetic

<400>  21
gctgctacac tctctgcaga gaaggtcacc ctgaaccagc gtgactatga gtactc           56


<210>  22
<211>  54
<212>  DNA
<213>  Synthetic

<400>  22
ggcactccac tgagtactca tagcacgctg gttcagggtg accttctctg caga             54


<210>  23
<211>  49
<212>  DNA
<213>  Synthetic

<400>  23
ggtccactga tattttaaag aacttcaaaa ataagacctt tctaagatg                   49


<210>  24
<211>  49
<212>  DNA
<213>  Synthetic

<400>  24
catcttagaa aggtcttatt tttgaagttc tttaaaatat cagtggacc                   49


<210>  25
<211>  45
<212>  DNA
<213>  Synthetic

<400>  25
gtccactgat attttaaagg accccaaaaa taagaccttt ctaag                       45


<210>  26
<211>  45
<212>  DNA
<213>  Synthetic

<400>  26
cttagaaagg tcttattttt ggggtccttt aaaatatcag tggac                       45


