                         SEQUENCE LISTING

<110>  Cargill, Incorporated
       Finley, Kenneth R
       Huryta, Jeanette M
       Mastel, Beth M
       McMullin, Thomas W
       Poynter, Gregory M
       Rush, Brian J
       Watts, Kevin T
       Fosmer, Arlene M
 
<120>  Compositions and Methods for Malate and Fumarate Production

<130>  33449.8058.WO00

<150>  61/436,186
<151>  2011-01-25

<160>  142   

<170>  PatentIn version 3.5

<210>  1
<211>  1458
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1458)

<400>  1
atg tta gct gct aga tca tta aag gca aga atg tca aca aga gct ttc         48
Met Leu Ala Ala Arg Ser Leu Lys Ala Arg Met Ser Thr Arg Ala Phe           
1               5                   10                  15                

tca act acc tca att gca aaa aga atc gaa aaa gat gca ttt ggt gac         96
Ser Thr Thr Ser Ile Ala Lys Arg Ile Glu Lys Asp Ala Phe Gly Asp           
            20                  25                  30                    

att gaa gtc cca aat gag aaa tat tgg ggt gct caa act caa aga tct        144
Ile Glu Val Pro Asn Glu Lys Tyr Trp Gly Ala Gln Thr Gln Arg Ser           
        35                  40                  45                        

tta caa aat ttc aaa att ggt ggt aag aga gaa gtt atg cca gaa cca        192
Leu Gln Asn Phe Lys Ile Gly Gly Lys Arg Glu Val Met Pro Glu Pro           
    50                  55                  60                            

atc atc aaa tct ttt ggt att tta aag aag gct act gct aag atc aat        240
Ile Ile Lys Ser Phe Gly Ile Leu Lys Lys Ala Thr Ala Lys Ile Asn           
65                  70                  75                  80            

gct gag tct ggt gct tta gac cca aag tta tct gaa gcc atc caa caa        288
Ala Glu Ser Gly Ala Leu Asp Pro Lys Leu Ser Glu Ala Ile Gln Gln           
                85                  90                  95                

gct gca acc gaa gtt tat gaa ggt aaa cta atg gac cat ttc cca tta        336
Ala Ala Thr Glu Val Tyr Glu Gly Lys Leu Met Asp His Phe Pro Leu           
            100                 105                 110                   

gtt gtc ttt caa acc ggt tct ggt act caa tct aac atg aat gcc aat        384
Val Val Phe Gln Thr Gly Ser Gly Thr Gln Ser Asn Met Asn Ala Asn           
        115                 120                 125                       

gaa gtc atc tct aat aga gca att gaa atc ttg ggt ggt gaa tta ggc        432
Glu Val Ile Ser Asn Arg Ala Ile Glu Ile Leu Gly Gly Glu Leu Gly           
    130                 135                 140                           

tct aaa act cca gtc cat cct aat gat cat gtt aat atg tcc caa tct        480
Ser Lys Thr Pro Val His Pro Asn Asp His Val Asn Met Ser Gln Ser           
145                 150                 155                 160           

tct aat gat act ttc cct act gtc atg cat att gca gca gtt aca gaa        528
Ser Asn Asp Thr Phe Pro Thr Val Met His Ile Ala Ala Val Thr Glu           
                165                 170                 175               

gtt tca tcc cat tta tta cca gaa tta act gca cta aga gat gca ttg        576
Val Ser Ser His Leu Leu Pro Glu Leu Thr Ala Leu Arg Asp Ala Leu           
            180                 185                 190                   

caa aag aaa tcc gat gaa ttt aag aat att atc aaa atc ggt aga acc        624
Gln Lys Lys Ser Asp Glu Phe Lys Asn Ile Ile Lys Ile Gly Arg Thr           
        195                 200                 205                       

cat tta caa gat gca act cct tta act tta ggt caa gaa ttt tct ggt        672
His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu Phe Ser Gly           
    210                 215                 220                           

tat gtt caa caa tgt act aat ggt atc aaa aga atc gaa att gct ctt        720
Tyr Val Gln Gln Cys Thr Asn Gly Ile Lys Arg Ile Glu Ile Ala Leu           
225                 230                 235                 240           

gaa cat ttg aga tac tta gct caa ggt ggt act gcc gtt ggt act ggt        768
Glu His Leu Arg Tyr Leu Ala Gln Gly Gly Thr Ala Val Gly Thr Gly           
                245                 250                 255               

ctt aac acc aag aaa ggt ttt gct gaa aag gtt gca aat gaa gtc act        816
Leu Asn Thr Lys Lys Gly Phe Ala Glu Lys Val Ala Asn Glu Val Thr           
            260                 265                 270                   

aaa ttg act ggt tta caa ttc tat acc gct cca aat aaa ttc gaa gcc        864
Lys Leu Thr Gly Leu Gln Phe Tyr Thr Ala Pro Asn Lys Phe Glu Ala           
        275                 280                 285                       

ctt gca gct cac gat gct gtt gtt gaa atg tct ggt gct ttg aat acc        912
Leu Ala Ala His Asp Ala Val Val Glu Met Ser Gly Ala Leu Asn Thr           
    290                 295                 300                           

gtt gca gtc tca tta ttc aaa atc gct caa gat atc aga tat ttg ggt        960
Val Ala Val Ser Leu Phe Lys Ile Ala Gln Asp Ile Arg Tyr Leu Gly           
305                 310                 315                 320           

tcc ggc cca aga tgt ggt tat ggt gaa ttg gct tta cca gaa aat gaa       1008
Ser Gly Pro Arg Cys Gly Tyr Gly Glu Leu Ala Leu Pro Glu Asn Glu           
                325                 330                 335               

cca ggt tct tcc atc atg ccg ggt aaa gtt aac cca act caa aac gaa       1056
Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr Gln Asn Glu           
            340                 345                 350                   

gct ttg act atg ctt tgt acc caa gtc ttt ggt aac cac tct tgt att       1104
Ala Leu Thr Met Leu Cys Thr Gln Val Phe Gly Asn His Ser Cys Ile           
        355                 360                 365                       

acc ttt gca ggt gct tca ggt caa ttc gaa ttg aat gtc ttt aag cca       1152
Thr Phe Ala Gly Ala Ser Gly Gln Phe Glu Leu Asn Val Phe Lys Pro           
    370                 375                 380                           

gtt atg atc tcc aac ttg tta tct tct att agg tta tta ggt gat ggt       1200
Val Met Ile Ser Asn Leu Leu Ser Ser Ile Arg Leu Leu Gly Asp Gly           
385                 390                 395                 400           

tgt aat tct ttt aga atc cac tgt gtt gaa ggt atc att gca aat acc       1248
Cys Asn Ser Phe Arg Ile His Cys Val Glu Gly Ile Ile Ala Asn Thr           
                405                 410                 415               

gac aag att gat aaa tta cta cat gaa tct ctc atg tta gtt act gct       1296
Asp Lys Ile Asp Lys Leu Leu His Glu Ser Leu Met Leu Val Thr Ala           
            420                 425                 430                   

ttg aac cca cac att ggt tac gat aag gct tcc aag att gca aag aat       1344
Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ser Lys Ile Ala Lys Asn           
        435                 440                 445                       

gca cac aag aag ggc ttg aca ttg aaa caa tct gca ttg gaa tta ggt       1392
Ala His Lys Lys Gly Leu Thr Leu Lys Gln Ser Ala Leu Glu Leu Gly           
    450                 455                 460                           

tac ttg acc gaa gaa caa ttc aat gaa tgg gtt aga cca gaa aac atg       1440
Tyr Leu Thr Glu Glu Gln Phe Asn Glu Trp Val Arg Pro Glu Asn Met           
465                 470                 475                 480           

att ggt cca aag gat taa                                               1458
Ile Gly Pro Lys Asp                                                       
                485                                                       


<210>  2
<211>  485
<212>  PRT
<213>  Issatchenkia orientalis

<400>  2

Met Leu Ala Ala Arg Ser Leu Lys Ala Arg Met Ser Thr Arg Ala Phe 
1               5                   10                  15      


Ser Thr Thr Ser Ile Ala Lys Arg Ile Glu Lys Asp Ala Phe Gly Asp 
            20                  25                  30          


Ile Glu Val Pro Asn Glu Lys Tyr Trp Gly Ala Gln Thr Gln Arg Ser 
        35                  40                  45              


Leu Gln Asn Phe Lys Ile Gly Gly Lys Arg Glu Val Met Pro Glu Pro 
    50                  55                  60                  


Ile Ile Lys Ser Phe Gly Ile Leu Lys Lys Ala Thr Ala Lys Ile Asn 
65                  70                  75                  80  


Ala Glu Ser Gly Ala Leu Asp Pro Lys Leu Ser Glu Ala Ile Gln Gln 
                85                  90                  95      


Ala Ala Thr Glu Val Tyr Glu Gly Lys Leu Met Asp His Phe Pro Leu 
            100                 105                 110         


Val Val Phe Gln Thr Gly Ser Gly Thr Gln Ser Asn Met Asn Ala Asn 
        115                 120                 125             


Glu Val Ile Ser Asn Arg Ala Ile Glu Ile Leu Gly Gly Glu Leu Gly 
    130                 135                 140                 


Ser Lys Thr Pro Val His Pro Asn Asp His Val Asn Met Ser Gln Ser 
145                 150                 155                 160 


Ser Asn Asp Thr Phe Pro Thr Val Met His Ile Ala Ala Val Thr Glu 
                165                 170                 175     


Val Ser Ser His Leu Leu Pro Glu Leu Thr Ala Leu Arg Asp Ala Leu 
            180                 185                 190         


Gln Lys Lys Ser Asp Glu Phe Lys Asn Ile Ile Lys Ile Gly Arg Thr 
        195                 200                 205             


His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu Phe Ser Gly 
    210                 215                 220                 


Tyr Val Gln Gln Cys Thr Asn Gly Ile Lys Arg Ile Glu Ile Ala Leu 
225                 230                 235                 240 


Glu His Leu Arg Tyr Leu Ala Gln Gly Gly Thr Ala Val Gly Thr Gly 
                245                 250                 255     


Leu Asn Thr Lys Lys Gly Phe Ala Glu Lys Val Ala Asn Glu Val Thr 
            260                 265                 270         


Lys Leu Thr Gly Leu Gln Phe Tyr Thr Ala Pro Asn Lys Phe Glu Ala 
        275                 280                 285             


Leu Ala Ala His Asp Ala Val Val Glu Met Ser Gly Ala Leu Asn Thr 
    290                 295                 300                 


Val Ala Val Ser Leu Phe Lys Ile Ala Gln Asp Ile Arg Tyr Leu Gly 
305                 310                 315                 320 


Ser Gly Pro Arg Cys Gly Tyr Gly Glu Leu Ala Leu Pro Glu Asn Glu 
                325                 330                 335     


Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr Gln Asn Glu 
            340                 345                 350         


Ala Leu Thr Met Leu Cys Thr Gln Val Phe Gly Asn His Ser Cys Ile 
        355                 360                 365             


Thr Phe Ala Gly Ala Ser Gly Gln Phe Glu Leu Asn Val Phe Lys Pro 
    370                 375                 380                 


Val Met Ile Ser Asn Leu Leu Ser Ser Ile Arg Leu Leu Gly Asp Gly 
385                 390                 395                 400 


Cys Asn Ser Phe Arg Ile His Cys Val Glu Gly Ile Ile Ala Asn Thr 
                405                 410                 415     


Asp Lys Ile Asp Lys Leu Leu His Glu Ser Leu Met Leu Val Thr Ala 
            420                 425                 430         


Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ser Lys Ile Ala Lys Asn 
        435                 440                 445             


Ala His Lys Lys Gly Leu Thr Leu Lys Gln Ser Ala Leu Glu Leu Gly 
    450                 455                 460                 


Tyr Leu Thr Glu Glu Gln Phe Asn Glu Trp Val Arg Pro Glu Asn Met 
465                 470                 475                 480 


Ile Gly Pro Lys Asp 
                485 


<210>  3
<211>  2652
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(2652)

<400>  3
atg aac gaa caa tat tcc gca ttg cgt agt aat gtc agt atg ctc ggc         48
Met Asn Glu Gln Tyr Ser Ala Leu Arg Ser Asn Val Ser Met Leu Gly           
1               5                   10                  15                

aaa gtg ctg gga gaa acc atc aag gat gcg ttg gga gaa cac att ctt         96
Lys Val Leu Gly Glu Thr Ile Lys Asp Ala Leu Gly Glu His Ile Leu           
            20                  25                  30                    

gaa cgc gta gaa act atc cgt aag ttg tcg aaa tct tca cgc gct ggc        144
Glu Arg Val Glu Thr Ile Arg Lys Leu Ser Lys Ser Ser Arg Ala Gly           
        35                  40                  45                        

aat gat gct aac cgc cag gag ttg ctc acc acc tta caa aat ttg tcg        192
Asn Asp Ala Asn Arg Gln Glu Leu Leu Thr Thr Leu Gln Asn Leu Ser           
    50                  55                  60                            

aac gac gag ctg ctg ccc gtt gcg cgt gcg ttt agt cag ttc ctg aac        240
Asn Asp Glu Leu Leu Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn           
65                  70                  75                  80            

ctg gcc aac acc gcc gag caa tac cac agc att tcg ccg aaa ggc gaa        288
Leu Ala Asn Thr Ala Glu Gln Tyr His Ser Ile Ser Pro Lys Gly Glu           
                85                  90                  95                

gct gcc agc aac ccg gaa gtg atc gcc cgc acc ctg cgt aaa ctg aaa        336
Ala Ala Ser Asn Pro Glu Val Ile Ala Arg Thr Leu Arg Lys Leu Lys           
            100                 105                 110                   

aac cag ccg gaa ctg agc gaa gac acc atc aaa aaa gca gtg gaa tcg        384
Asn Gln Pro Glu Leu Ser Glu Asp Thr Ile Lys Lys Ala Val Glu Ser           
        115                 120                 125                       

ctg tcg ctg gaa ctg gtc ctc acg gct cac cca acc gaa att acc cgt        432
Leu Ser Leu Glu Leu Val Leu Thr Ala His Pro Thr Glu Ile Thr Arg           
    130                 135                 140                           

cgt aca ctg atc cac aaa atg gtg gaa gtg aac gcc tgt tta aaa cag        480
Arg Thr Leu Ile His Lys Met Val Glu Val Asn Ala Cys Leu Lys Gln           
145                 150                 155                 160           

ctc gat aac aaa gat atc gct gac tac gaa cac aac cag ctg atg cgt        528
Leu Asp Asn Lys Asp Ile Ala Asp Tyr Glu His Asn Gln Leu Met Arg           
                165                 170                 175               

cgc ctg cgc cag ttg atc gcc cag tca tgg cat acc gat gaa atc cgt        576
Arg Leu Arg Gln Leu Ile Ala Gln Ser Trp His Thr Asp Glu Ile Arg           
            180                 185                 190                   

aag ctg cgt cca agc ccg gta gat gaa gcc aaa tgg ggc ttt gcc gta        624
Lys Leu Arg Pro Ser Pro Val Asp Glu Ala Lys Trp Gly Phe Ala Val           
        195                 200                 205                       

gtg gaa aac agc ctg tgg caa ggc gta cca aat tac ctg cgc gaa ctg        672
Val Glu Asn Ser Leu Trp Gln Gly Val Pro Asn Tyr Leu Arg Glu Leu           
    210                 215                 220                           

aac gaa caa ctg gaa gag aac ctc ggc tac aaa ctg ccc gtc gaa ttt        720
Asn Glu Gln Leu Glu Glu Asn Leu Gly Tyr Lys Leu Pro Val Glu Phe           
225                 230                 235                 240           

gtt ccg gtc cgt ttt act tcg tgg atg ggc ggc gac cgc gac ggc aac        768
Val Pro Val Arg Phe Thr Ser Trp Met Gly Gly Asp Arg Asp Gly Asn           
                245                 250                 255               

ccg aac gtc act gcc gat atc acc cgc cac gtc ctg cta ctc agc cgc        816
Pro Asn Val Thr Ala Asp Ile Thr Arg His Val Leu Leu Leu Ser Arg           
            260                 265                 270                   

tgg aaa gcc acc gat ttg ttc ctg aaa gat att cag gtg ctg gtt tct        864
Trp Lys Ala Thr Asp Leu Phe Leu Lys Asp Ile Gln Val Leu Val Ser           
        275                 280                 285                       

gaa ctg tcg atg gtt gaa gcg acc cct gaa ctg ctg gcg ctg gtt ggc        912
Glu Leu Ser Met Val Glu Ala Thr Pro Glu Leu Leu Ala Leu Val Gly           
    290                 295                 300                           

gaa gaa ggt gcc gca gaa ccg tat cgc tat ctg atg aaa aac ctg cgt        960
Glu Glu Gly Ala Ala Glu Pro Tyr Arg Tyr Leu Met Lys Asn Leu Arg           
305                 310                 315                 320           

tct cgc ctg atg gcg aca cag gca tgg ctg gaa gcg cgc ctg aaa ggc       1008
Ser Arg Leu Met Ala Thr Gln Ala Trp Leu Glu Ala Arg Leu Lys Gly           
                325                 330                 335               

gaa gaa ctg cca aaa cca gaa ggc ctg ctg aca caa aac gaa gaa ctg       1056
Glu Glu Leu Pro Lys Pro Glu Gly Leu Leu Thr Gln Asn Glu Glu Leu           
            340                 345                 350                   

tgg gaa ccg ctc tac gct tgc tac cag tca ctt cag gcg tgt ggc atg       1104
Trp Glu Pro Leu Tyr Ala Cys Tyr Gln Ser Leu Gln Ala Cys Gly Met           
        355                 360                 365                       

ggt att atc gcc aac ggc gat ctg ctc gac acc ctg cgc cgc gtg aaa       1152
Gly Ile Ile Ala Asn Gly Asp Leu Leu Asp Thr Leu Arg Arg Val Lys           
    370                 375                 380                           

tgt ttc ggc gta ccg ctg gtc cgt att gat atc cgt cag gag agc acg       1200
Cys Phe Gly Val Pro Leu Val Arg Ile Asp Ile Arg Gln Glu Ser Thr           
385                 390                 395                 400           

cgt cat acc gaa gcg ctg ggc gag ctg acc cgc tac ctc ggt atc ggc       1248
Arg His Thr Glu Ala Leu Gly Glu Leu Thr Arg Tyr Leu Gly Ile Gly           
                405                 410                 415               

gac tac gaa agc tgg tca gag gcc gac aaa cag gcg ttc ctg atc cgc       1296
Asp Tyr Glu Ser Trp Ser Glu Ala Asp Lys Gln Ala Phe Leu Ile Arg           
            420                 425                 430                   

gaa ctg aac tcc aaa cgt ccg ctt ctg ccg cgc aac tgg caa cca agc       1344
Glu Leu Asn Ser Lys Arg Pro Leu Leu Pro Arg Asn Trp Gln Pro Ser           
        435                 440                 445                       

gcc gaa acg cgc gaa gtg ctc gat acc tgc cag gtg att gcc gaa gca       1392
Ala Glu Thr Arg Glu Val Leu Asp Thr Cys Gln Val Ile Ala Glu Ala           
    450                 455                 460                           

ccg caa ggc tcc att gcc gcc tac gtg atc tcg atg gcg aaa acg ccg       1440
Pro Gln Gly Ser Ile Ala Ala Tyr Val Ile Ser Met Ala Lys Thr Pro           
465                 470                 475                 480           

tcc gac gta ctg gct gtc cac ctg ctg ctg aaa gaa gcg ggt atc ggg       1488
Ser Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Gly           
                485                 490                 495               

ttt gcg atg ccg gtt gct ccg ctg ttt gaa acc ctc gat gat ctg aac       1536
Phe Ala Met Pro Val Ala Pro Leu Phe Glu Thr Leu Asp Asp Leu Asn           
            500                 505                 510                   

aac gcc aac gat gtc atg acc cag ctg ctc aat att gac tgg tat cgt       1584
Asn Ala Asn Asp Val Met Thr Gln Leu Leu Asn Ile Asp Trp Tyr Arg           
        515                 520                 525                       

ggc ctg att cag ggc aaa cag atg gtg atg att ggc tat tcc gac tca       1632
Gly Leu Ile Gln Gly Lys Gln Met Val Met Ile Gly Tyr Ser Asp Ser           
    530                 535                 540                           

gca aaa gat gcg gga gtg atg gca gct tcc tgg gcg caa tat cag gca       1680
Ala Lys Asp Ala Gly Val Met Ala Ala Ser Trp Ala Gln Tyr Gln Ala           
545                 550                 555                 560           

cag gat gca tta atc aaa acc tgc gaa aaa gcg ggt att gag ctg acg       1728
Gln Asp Ala Leu Ile Lys Thr Cys Glu Lys Ala Gly Ile Glu Leu Thr           
                565                 570                 575               

ttg ttc cac ggt cgc ggc ggt tcc att ggt cgc ggc ggc gca cct gct       1776
Leu Phe His Gly Arg Gly Gly Ser Ile Gly Arg Gly Gly Ala Pro Ala           
            580                 585                 590                   

cat gcg gcg ctg ctg tca caa ccg cca gga agc ctg aaa ggc ggc ctg       1824
His Ala Ala Leu Leu Ser Gln Pro Pro Gly Ser Leu Lys Gly Gly Leu           
        595                 600                 605                       

cgc gta acc gaa cag ggc gag atg atc cgc ttt aaa tat ggt ctg cca       1872
Arg Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Tyr Gly Leu Pro           
    610                 615                 620                           

gaa atc acc gtc agc agc ctg tcg ctt tat acc ggg gcg att ctg gaa       1920
Glu Ile Thr Val Ser Ser Leu Ser Leu Tyr Thr Gly Ala Ile Leu Glu           
625                 630                 635                 640           

gcc aac ctg ctg cca ccg ccg gag ccg aaa gag agc tgg cgt cgc att       1968
Ala Asn Leu Leu Pro Pro Pro Glu Pro Lys Glu Ser Trp Arg Arg Ile           
                645                 650                 655               

atg gat gaa ctg tca gtc atc tcc tgc gat gtc tac cgc ggc tac gta       2016
Met Asp Glu Leu Ser Val Ile Ser Cys Asp Val Tyr Arg Gly Tyr Val           
            660                 665                 670                   

cgt gaa aac aaa gat ttt gtg cct tac ttc cgc tcc gct acg ccg gaa       2064
Arg Glu Asn Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu           
        675                 680                 685                       

caa gaa ctg ggc aaa ctg ccg ttg ggt tca cgt ccg gcg aaa cgt cgc       2112
Gln Glu Leu Gly Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Arg           
    690                 695                 700                           

cca acc ggc ggc gtc gag tca cta cgc gcc att ccg tgg atc ttc gcc       2160
Pro Thr Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala           
705                 710                 715                 720           

tgg acg caa aac cgt ctg atg ctc ccc gcc tgg ctg ggt gca ggt acg       2208
Trp Thr Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Thr           
                725                 730                 735               

gcg ctg caa aaa gtg gtc gaa gac ggc aaa cag agc gag ctg gag gct       2256
Ala Leu Gln Lys Val Val Glu Asp Gly Lys Gln Ser Glu Leu Glu Ala           
            740                 745                 750                   

atg tgc cgc gat tgg cca ttc ttc tcg acg cgt ctc ggc atg ctg gag       2304
Met Cys Arg Asp Trp Pro Phe Phe Ser Thr Arg Leu Gly Met Leu Glu           
        755                 760                 765                       

atg gtc ttc gcc aaa gca gac ctg tgg ctg gcg gaa tac tat gac caa       2352
Met Val Phe Ala Lys Ala Asp Leu Trp Leu Ala Glu Tyr Tyr Asp Gln           
    770                 775                 780                           

cgc ctg gta gac aaa gca ctg tgg ccg tta ggt aaa gag tta cgc aac       2400
Arg Leu Val Asp Lys Ala Leu Trp Pro Leu Gly Lys Glu Leu Arg Asn           
785                 790                 795                 800           

ctg caa gaa gaa gac atc aaa gtg gtg ctg gcg att gcc aac gat tcc       2448
Leu Gln Glu Glu Asp Ile Lys Val Val Leu Ala Ile Ala Asn Asp Ser           
                805                 810                 815               

cat ctg atg gcc gat ctg ccg tgg att gca gag tct att cag cta cgg       2496
His Leu Met Ala Asp Leu Pro Trp Ile Ala Glu Ser Ile Gln Leu Arg           
            820                 825                 830                   

aat att tac acc gac ccg ctg aac gta ttg cag gcc gag ttg ctg cac       2544
Asn Ile Tyr Thr Asp Pro Leu Asn Val Leu Gln Ala Glu Leu Leu His           
        835                 840                 845                       

cgc tcc cgc cag gca gaa aaa gaa ggc cag gaa ccg gat cct cgc gtc       2592
Arg Ser Arg Gln Ala Glu Lys Glu Gly Gln Glu Pro Asp Pro Arg Val           
    850                 855                 860                           

gaa caa gcg tta atg gtc act att gcc ggg att gcg gca ggt atg cgt       2640
Glu Gln Ala Leu Met Val Thr Ile Ala Gly Ile Ala Ala Gly Met Arg           
865                 870                 875                 880           

aat acc ggc taa                                                       2652
Asn Thr Gly                                                               
                                                                          


<210>  4
<211>  883
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Asn Glu Gln Tyr Ser Ala Leu Arg Ser Asn Val Ser Met Leu Gly 
1               5                   10                  15      


Lys Val Leu Gly Glu Thr Ile Lys Asp Ala Leu Gly Glu His Ile Leu 
            20                  25                  30          


Glu Arg Val Glu Thr Ile Arg Lys Leu Ser Lys Ser Ser Arg Ala Gly 
        35                  40                  45              


Asn Asp Ala Asn Arg Gln Glu Leu Leu Thr Thr Leu Gln Asn Leu Ser 
    50                  55                  60                  


Asn Asp Glu Leu Leu Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn 
65                  70                  75                  80  


Leu Ala Asn Thr Ala Glu Gln Tyr His Ser Ile Ser Pro Lys Gly Glu 
                85                  90                  95      


Ala Ala Ser Asn Pro Glu Val Ile Ala Arg Thr Leu Arg Lys Leu Lys 
            100                 105                 110         


Asn Gln Pro Glu Leu Ser Glu Asp Thr Ile Lys Lys Ala Val Glu Ser 
        115                 120                 125             


Leu Ser Leu Glu Leu Val Leu Thr Ala His Pro Thr Glu Ile Thr Arg 
    130                 135                 140                 


Arg Thr Leu Ile His Lys Met Val Glu Val Asn Ala Cys Leu Lys Gln 
145                 150                 155                 160 


Leu Asp Asn Lys Asp Ile Ala Asp Tyr Glu His Asn Gln Leu Met Arg 
                165                 170                 175     


Arg Leu Arg Gln Leu Ile Ala Gln Ser Trp His Thr Asp Glu Ile Arg 
            180                 185                 190         


Lys Leu Arg Pro Ser Pro Val Asp Glu Ala Lys Trp Gly Phe Ala Val 
        195                 200                 205             


Val Glu Asn Ser Leu Trp Gln Gly Val Pro Asn Tyr Leu Arg Glu Leu 
    210                 215                 220                 


Asn Glu Gln Leu Glu Glu Asn Leu Gly Tyr Lys Leu Pro Val Glu Phe 
225                 230                 235                 240 


Val Pro Val Arg Phe Thr Ser Trp Met Gly Gly Asp Arg Asp Gly Asn 
                245                 250                 255     


Pro Asn Val Thr Ala Asp Ile Thr Arg His Val Leu Leu Leu Ser Arg 
            260                 265                 270         


Trp Lys Ala Thr Asp Leu Phe Leu Lys Asp Ile Gln Val Leu Val Ser 
        275                 280                 285             


Glu Leu Ser Met Val Glu Ala Thr Pro Glu Leu Leu Ala Leu Val Gly 
    290                 295                 300                 


Glu Glu Gly Ala Ala Glu Pro Tyr Arg Tyr Leu Met Lys Asn Leu Arg 
305                 310                 315                 320 


Ser Arg Leu Met Ala Thr Gln Ala Trp Leu Glu Ala Arg Leu Lys Gly 
                325                 330                 335     


Glu Glu Leu Pro Lys Pro Glu Gly Leu Leu Thr Gln Asn Glu Glu Leu 
            340                 345                 350         


Trp Glu Pro Leu Tyr Ala Cys Tyr Gln Ser Leu Gln Ala Cys Gly Met 
        355                 360                 365             


Gly Ile Ile Ala Asn Gly Asp Leu Leu Asp Thr Leu Arg Arg Val Lys 
    370                 375                 380                 


Cys Phe Gly Val Pro Leu Val Arg Ile Asp Ile Arg Gln Glu Ser Thr 
385                 390                 395                 400 


Arg His Thr Glu Ala Leu Gly Glu Leu Thr Arg Tyr Leu Gly Ile Gly 
                405                 410                 415     


Asp Tyr Glu Ser Trp Ser Glu Ala Asp Lys Gln Ala Phe Leu Ile Arg 
            420                 425                 430         


Glu Leu Asn Ser Lys Arg Pro Leu Leu Pro Arg Asn Trp Gln Pro Ser 
        435                 440                 445             


Ala Glu Thr Arg Glu Val Leu Asp Thr Cys Gln Val Ile Ala Glu Ala 
    450                 455                 460                 


Pro Gln Gly Ser Ile Ala Ala Tyr Val Ile Ser Met Ala Lys Thr Pro 
465                 470                 475                 480 


Ser Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Gly 
                485                 490                 495     


Phe Ala Met Pro Val Ala Pro Leu Phe Glu Thr Leu Asp Asp Leu Asn 
            500                 505                 510         


Asn Ala Asn Asp Val Met Thr Gln Leu Leu Asn Ile Asp Trp Tyr Arg 
        515                 520                 525             


Gly Leu Ile Gln Gly Lys Gln Met Val Met Ile Gly Tyr Ser Asp Ser 
    530                 535                 540                 


Ala Lys Asp Ala Gly Val Met Ala Ala Ser Trp Ala Gln Tyr Gln Ala 
545                 550                 555                 560 


Gln Asp Ala Leu Ile Lys Thr Cys Glu Lys Ala Gly Ile Glu Leu Thr 
                565                 570                 575     


Leu Phe His Gly Arg Gly Gly Ser Ile Gly Arg Gly Gly Ala Pro Ala 
            580                 585                 590         


His Ala Ala Leu Leu Ser Gln Pro Pro Gly Ser Leu Lys Gly Gly Leu 
        595                 600                 605             


Arg Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Tyr Gly Leu Pro 
    610                 615                 620                 


Glu Ile Thr Val Ser Ser Leu Ser Leu Tyr Thr Gly Ala Ile Leu Glu 
625                 630                 635                 640 


Ala Asn Leu Leu Pro Pro Pro Glu Pro Lys Glu Ser Trp Arg Arg Ile 
                645                 650                 655     


Met Asp Glu Leu Ser Val Ile Ser Cys Asp Val Tyr Arg Gly Tyr Val 
            660                 665                 670         


Arg Glu Asn Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu 
        675                 680                 685             


Gln Glu Leu Gly Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Arg 
    690                 695                 700                 


Pro Thr Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala 
705                 710                 715                 720 


Trp Thr Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Thr 
                725                 730                 735     


Ala Leu Gln Lys Val Val Glu Asp Gly Lys Gln Ser Glu Leu Glu Ala 
            740                 745                 750         


Met Cys Arg Asp Trp Pro Phe Phe Ser Thr Arg Leu Gly Met Leu Glu 
        755                 760                 765             


Met Val Phe Ala Lys Ala Asp Leu Trp Leu Ala Glu Tyr Tyr Asp Gln 
    770                 775                 780                 


Arg Leu Val Asp Lys Ala Leu Trp Pro Leu Gly Lys Glu Leu Arg Asn 
785                 790                 795                 800 


Leu Gln Glu Glu Asp Ile Lys Val Val Leu Ala Ile Ala Asn Asp Ser 
                805                 810                 815     


His Leu Met Ala Asp Leu Pro Trp Ile Ala Glu Ser Ile Gln Leu Arg 
            820                 825                 830         


Asn Ile Tyr Thr Asp Pro Leu Asn Val Leu Gln Ala Glu Leu Leu His 
        835                 840                 845             


Arg Ser Arg Gln Ala Glu Lys Glu Gly Gln Glu Pro Asp Pro Arg Val 
    850                 855                 860                 


Glu Gln Ala Leu Met Val Thr Ile Ala Gly Ile Ala Ala Gly Met Arg 
865                 870                 875                 880 


Asn Thr Gly 
            


<210>  5
<211>  2643
<212>  DNA
<213>  Mannheimia succiniciproducens


<220>
<221>  CDS
<222>  (1)..(2643)

<400>  5
atg aca gaa gaa tat tta atg atg cgt aat aac atc aat atg ctg ggg         48
Met Thr Glu Glu Tyr Leu Met Met Arg Asn Asn Ile Asn Met Leu Gly           
1               5                   10                  15                

cgc ttt ttg ggc gaa act att cag gag gcg caa ggt gac gat att ctc         96
Arg Phe Leu Gly Glu Thr Ile Gln Glu Ala Gln Gly Asp Asp Ile Leu           
            20                  25                  30                    

gaa ctg att gaa aat atc cgc gta ctg tcc cgc aat tcc cgt agc ggc        144
Glu Leu Ile Glu Asn Ile Arg Val Leu Ser Arg Asn Ser Arg Ser Gly           
        35                  40                  45                        

gat gac aaa gcc cgg gcg gca tta tta gac acc ctt tcc act att tcg        192
Asp Asp Lys Ala Arg Ala Ala Leu Leu Asp Thr Leu Ser Thr Ile Ser           
    50                  55                  60                            

gcg gat aat att att ccg gtt gcc cgc gct ttc agc cag ttt ctg aac        240
Ala Asp Asn Ile Ile Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn           
65                  70                  75                  80            

ctg aca aat gtg gcg gaa caa tat caa acc atg tct cgc tcc cat gaa        288
Leu Thr Asn Val Ala Glu Gln Tyr Gln Thr Met Ser Arg Ser His Glu           
                85                  90                  95                

gat aag gtt tct gcg gaa cgt tcc act gct gcg ctg ttc gcc cgc ctg        336
Asp Lys Val Ser Ala Glu Arg Ser Thr Ala Ala Leu Phe Ala Arg Leu           
            100                 105                 110                   

aaa gaa caa cat gtt tct cag gaa gaa atc att aaa acc gta cag aaa        384
Lys Glu Gln His Val Ser Gln Glu Glu Ile Ile Lys Thr Val Gln Lys           
        115                 120                 125                       

ctg ttg att gaa atc gtc ctt acc gct cac ccg acg gaa gtt acc cgc        432
Leu Leu Ile Glu Ile Val Leu Thr Ala His Pro Thr Glu Val Thr Arg           
    130                 135                 140                           

cgt tca tta atg cac aaa cag gtt gaa atc aac aaa tgt ctg gct cag        480
Arg Ser Leu Met His Lys Gln Val Glu Ile Asn Lys Cys Leu Ala Gln           
145                 150                 155                 160           

ctg gat cat acg gat tta acc gcc gaa gaa caa aaa aat att gag tat        528
Leu Asp His Thr Asp Leu Thr Ala Glu Glu Gln Lys Asn Ile Glu Tyr           
                165                 170                 175               

aaa tta ctt cgt ctt atc gcc gaa gcc tgg cat acc aat gaa atc cgt        576
Lys Leu Leu Arg Leu Ile Ala Glu Ala Trp His Thr Asn Glu Ile Arg           
            180                 185                 190                   

acc aat cgg ccg aca cct ctg gaa gaa gcc aaa tgg ggt ttt gcc gtt        624
Thr Asn Arg Pro Thr Pro Leu Glu Glu Ala Lys Trp Gly Phe Ala Val           
        195                 200                 205                       

atc gaa aac agt tta tgg gaa ggt ttg ccc gcc ttt atc cgc aaa ctt        672
Ile Glu Asn Ser Leu Trp Glu Gly Leu Pro Ala Phe Ile Arg Lys Leu           
    210                 215                 220                           

aac gat gcc gcc gtc gaa cat tta aat tat gct ttg ccg gta gac ctc        720
Asn Asp Ala Ala Val Glu His Leu Asn Tyr Ala Leu Pro Val Asp Leu           
225                 230                 235                 240           

aca ccg gta cgc ttc tct tcc tgg atg ggc ggt gac cgt gac ggc aac        768
Thr Pro Val Arg Phe Ser Ser Trp Met Gly Gly Asp Arg Asp Gly Asn           
                245                 250                 255               

ccc ttc gtt acc gca aaa att acc cgg gaa gcg ctg caa ctt gcg cgc        816
Pro Phe Val Thr Ala Lys Ile Thr Arg Glu Ala Leu Gln Leu Ala Arg           
            260                 265                 270                   

tgg aaa gcg gcg gat tta ttt tta acc gat att cag gaa ctc tgc gac        864
Trp Lys Ala Ala Asp Leu Phe Leu Thr Asp Ile Gln Glu Leu Cys Asp           
        275                 280                 285                       

gag ttg tca atg aca caa tgc act gcg gaa ttc cga gaa aaa tac ggt        912
Glu Leu Ser Met Thr Gln Cys Thr Ala Glu Phe Arg Glu Lys Tyr Gly           
    290                 295                 300                           

gat cat tta gaa ccc tat cgt gta gtt gtg aag gat tta cgc agc aaa        960
Asp His Leu Glu Pro Tyr Arg Val Val Val Lys Asp Leu Arg Ser Lys           
305                 310                 315                 320           

tta aaa aat acg ctg gat tat tac aac gat ata ctt gcg ggt cgc att       1008
Leu Lys Asn Thr Leu Asp Tyr Tyr Asn Asp Ile Leu Ala Gly Arg Ile           
                325                 330                 335               

ccg ccg ttt aaa caa gat gaa atc atc agt gaa gac caa caa ctc tgg       1056
Pro Pro Phe Lys Gln Asp Glu Ile Ile Ser Glu Asp Gln Gln Leu Trp           
            340                 345                 350                   

caa ccg ctt tat gac tgt tat caa tcc cta acc gcc tgc ggt atg cgt       1104
Gln Pro Leu Tyr Asp Cys Tyr Gln Ser Leu Thr Ala Cys Gly Met Arg           
        355                 360                 365                       

att att gcc aat gga tta ttg ctg gat acc tta cgc cgc gtt cgt tgt       1152
Ile Ile Ala Asn Gly Leu Leu Leu Asp Thr Leu Arg Arg Val Arg Cys           
    370                 375                 380                           

ttc ggc gtc aca tta ctg cgt tta gat atc cgt cag gaa agc acc cgc       1200
Phe Gly Val Thr Leu Leu Arg Leu Asp Ile Arg Gln Glu Ser Thr Arg           
385                 390                 395                 400           

cat agc gac gcc atc ggc gaa att acc cgc tac atc ggt tta ggc gat       1248
His Ser Asp Ala Ile Gly Glu Ile Thr Arg Tyr Ile Gly Leu Gly Asp           
                405                 410                 415               

tac agc caa tgg aca gaa gat gac aaa caa gcc ttc ctg atc cgg gaa       1296
Tyr Ser Gln Trp Thr Glu Asp Asp Lys Gln Ala Phe Leu Ile Arg Glu           
            420                 425                 430                   

tta agt tcc cgt cgt ccg cta att ccc cat aac tgg acg cct tcg gaa       1344
Leu Ser Ser Arg Arg Pro Leu Ile Pro His Asn Trp Thr Pro Ser Glu           
        435                 440                 445                       

cac act cgg gaa att tta gac acc tgt aaa gtc att gca aaa cag ccg       1392
His Thr Arg Glu Ile Leu Asp Thr Cys Lys Val Ile Ala Lys Gln Pro           
    450                 455                 460                           

gaa ggc gtt att tcc tgc tat atc att tcc atg gcg cgc acc gct tcc       1440
Glu Gly Val Ile Ser Cys Tyr Ile Ile Ser Met Ala Arg Thr Ala Ser           
465                 470                 475                 480           

gat gtt ttg gcg gtg cat tta tta ttg aaa gaa gcg ggc att tca tac       1488
Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Ser Tyr           
                485                 490                 495               

cat ctg ccg gta gtt cct cta ttt gaa aca ttg gac gac ctg gac gct       1536
His Leu Pro Val Val Pro Leu Phe Glu Thr Leu Asp Asp Leu Asp Ala           
            500                 505                 510                   

tct aaa gaa gtg atg acg caa ctg ttt aac gta ggc tgg tat cgc ggc       1584
Ser Lys Glu Val Met Thr Gln Leu Phe Asn Val Gly Trp Tyr Arg Gly           
        515                 520                 525                       

gta atc aaa aac cgc caa atg atc atg atc ggc tat tcc gat agc gcc       1632
Val Ile Lys Asn Arg Gln Met Ile Met Ile Gly Tyr Ser Asp Ser Ala           
    530                 535                 540                           

aaa gat gcg ggc atg atg gcg gcc tca tgg gcg caa tac cgg gcg cag       1680
Lys Asp Ala Gly Met Met Ala Ala Ser Trp Ala Gln Tyr Arg Ala Gln           
545                 550                 555                 560           

gac gct tta gtc aaa ctt tgc gaa caa acc ggc atc gaa ctt acc ctc       1728
Asp Ala Leu Val Lys Leu Cys Glu Gln Thr Gly Ile Glu Leu Thr Leu           
                565                 570                 575               

ttc cac ggc cgc ggc ggc acc gta gga cgt ggc ggt gca ccg gct cac       1776
Phe His Gly Arg Gly Gly Thr Val Gly Arg Gly Gly Ala Pro Ala His           
            580                 585                 590                   

gcc gca tta tta tcc caa ccg cca cgt tct ctg aaa aac ggc tta cgg       1824
Ala Ala Leu Leu Ser Gln Pro Pro Arg Ser Leu Lys Asn Gly Leu Arg           
        595                 600                 605                       

gta acc gaa caa ggg gaa atg atc cgc ttc aaa ctg gga tta ccg gct       1872
Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Leu Gly Leu Pro Ala           
    610                 615                 620                           

atc gcc gca gaa agt ctg gat ctc tac gcc agc gcc att ctt gag gcc       1920
Ile Ala Ala Glu Ser Leu Asp Leu Tyr Ala Ser Ala Ile Leu Glu Ala           
625                 630                 635                 640           

aac ctc ctg ccg ccg ccg gaa ccg aaa gcc agc tgg tgc cgg gta atg       1968
Asn Leu Leu Pro Pro Pro Glu Pro Lys Ala Ser Trp Cys Arg Val Met           
                645                 650                 655               

gac gaa ctt gcc gtc gct tct tgc gaa atc tat cgc aat gtg gtg cgc       2016
Asp Glu Leu Ala Val Ala Ser Cys Glu Ile Tyr Arg Asn Val Val Arg           
            660                 665                 670                   

ggc gat aaa gat ttt gtg cct tac ttc cgc agc gcc aca ccg gaa cag       2064
Gly Asp Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu Gln           
        675                 680                 685                       

gaa ctg gca aaa ctg cct tta ggt tcc cga ccg gca aaa cgc aat ccg       2112
Glu Leu Ala Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Asn Pro           
    690                 695                 700                           

aac ggc ggc gtt gaa agc ctg cgt gcc att ccc tgg atc ttc gcc tgg       2160
Asn Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala Trp           
705                 710                 715                 720           

atg caa aac cgc ctg atg ctg ccc gcc tgg ctc ggt gcc ggc gcc tca       2208
Met Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Ala Ser           
                725                 730                 735               

atc cgt cag gcg atg gaa agc ggc aaa gcg gcg gtg att gaa gaa atg       2256
Ile Arg Gln Ala Met Glu Ser Gly Lys Ala Ala Val Ile Glu Glu Met           
            740                 745                 750                   

tgc aac cat tgg ccg ttt ttc aat acc cga atc ggc atg ctt gaa atg       2304
Cys Asn His Trp Pro Phe Phe Asn Thr Arg Ile Gly Met Leu Glu Met           
        755                 760                 765                       

gta ttc agt aaa acc gat agc tgg ctg tcc gaa tat tac gac cag cgt       2352
Val Phe Ser Lys Thr Asp Ser Trp Leu Ser Glu Tyr Tyr Asp Gln Arg           
    770                 775                 780                           

tta gtg aaa aaa gag ctt tgg tat tta ggc gaa tcg ctg cgc aaa cag       2400
Leu Val Lys Lys Glu Leu Trp Tyr Leu Gly Glu Ser Leu Arg Lys Gln           
785                 790                 795                 800           

tta agc gaa gat atc gct acc gtg tta cgg ctt tcc ggc aaa ggc gat       2448
Leu Ser Glu Asp Ile Ala Thr Val Leu Arg Leu Ser Gly Lys Gly Asp           
                805                 810                 815               

caa tta atg tcg gat ttg cct tgg gtg gcg gaa tct att gca ctg cgt       2496
Gln Leu Met Ser Asp Leu Pro Trp Val Ala Glu Ser Ile Ala Leu Arg           
            820                 825                 830                   

aac gtt tac acc gac ccg tta aac tta ttg caa gtg gaa tta ttg cgt       2544
Asn Val Tyr Thr Asp Pro Leu Asn Leu Leu Gln Val Glu Leu Leu Arg           
        835                 840                 845                       

cgt ttg cga gcg gat ccc gaa cat ccg aat ccg gat atc gag caa gcg       2592
Arg Leu Arg Ala Asp Pro Glu His Pro Asn Pro Asp Ile Glu Gln Ala           
    850                 855                 860                           

ctg atg atc acc att acc ggt atc gcc gcg ggt atg cgt aat acg ggt       2640
Leu Met Ile Thr Ile Thr Gly Ile Ala Ala Gly Met Arg Asn Thr Gly           
865                 870                 875                 880           

tag                                                                   2643


<210>  6
<211>  880
<212>  PRT
<213>  Mannheimia succiniciproducens

<400>  6

Met Thr Glu Glu Tyr Leu Met Met Arg Asn Asn Ile Asn Met Leu Gly 
1               5                   10                  15      


Arg Phe Leu Gly Glu Thr Ile Gln Glu Ala Gln Gly Asp Asp Ile Leu 
            20                  25                  30          


Glu Leu Ile Glu Asn Ile Arg Val Leu Ser Arg Asn Ser Arg Ser Gly 
        35                  40                  45              


Asp Asp Lys Ala Arg Ala Ala Leu Leu Asp Thr Leu Ser Thr Ile Ser 
    50                  55                  60                  


Ala Asp Asn Ile Ile Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn 
65                  70                  75                  80  


Leu Thr Asn Val Ala Glu Gln Tyr Gln Thr Met Ser Arg Ser His Glu 
                85                  90                  95      


Asp Lys Val Ser Ala Glu Arg Ser Thr Ala Ala Leu Phe Ala Arg Leu 
            100                 105                 110         


Lys Glu Gln His Val Ser Gln Glu Glu Ile Ile Lys Thr Val Gln Lys 
        115                 120                 125             


Leu Leu Ile Glu Ile Val Leu Thr Ala His Pro Thr Glu Val Thr Arg 
    130                 135                 140                 


Arg Ser Leu Met His Lys Gln Val Glu Ile Asn Lys Cys Leu Ala Gln 
145                 150                 155                 160 


Leu Asp His Thr Asp Leu Thr Ala Glu Glu Gln Lys Asn Ile Glu Tyr 
                165                 170                 175     


Lys Leu Leu Arg Leu Ile Ala Glu Ala Trp His Thr Asn Glu Ile Arg 
            180                 185                 190         


Thr Asn Arg Pro Thr Pro Leu Glu Glu Ala Lys Trp Gly Phe Ala Val 
        195                 200                 205             


Ile Glu Asn Ser Leu Trp Glu Gly Leu Pro Ala Phe Ile Arg Lys Leu 
    210                 215                 220                 


Asn Asp Ala Ala Val Glu His Leu Asn Tyr Ala Leu Pro Val Asp Leu 
225                 230                 235                 240 


Thr Pro Val Arg Phe Ser Ser Trp Met Gly Gly Asp Arg Asp Gly Asn 
                245                 250                 255     


Pro Phe Val Thr Ala Lys Ile Thr Arg Glu Ala Leu Gln Leu Ala Arg 
            260                 265                 270         


Trp Lys Ala Ala Asp Leu Phe Leu Thr Asp Ile Gln Glu Leu Cys Asp 
        275                 280                 285             


Glu Leu Ser Met Thr Gln Cys Thr Ala Glu Phe Arg Glu Lys Tyr Gly 
    290                 295                 300                 


Asp His Leu Glu Pro Tyr Arg Val Val Val Lys Asp Leu Arg Ser Lys 
305                 310                 315                 320 


Leu Lys Asn Thr Leu Asp Tyr Tyr Asn Asp Ile Leu Ala Gly Arg Ile 
                325                 330                 335     


Pro Pro Phe Lys Gln Asp Glu Ile Ile Ser Glu Asp Gln Gln Leu Trp 
            340                 345                 350         


Gln Pro Leu Tyr Asp Cys Tyr Gln Ser Leu Thr Ala Cys Gly Met Arg 
        355                 360                 365             


Ile Ile Ala Asn Gly Leu Leu Leu Asp Thr Leu Arg Arg Val Arg Cys 
    370                 375                 380                 


Phe Gly Val Thr Leu Leu Arg Leu Asp Ile Arg Gln Glu Ser Thr Arg 
385                 390                 395                 400 


His Ser Asp Ala Ile Gly Glu Ile Thr Arg Tyr Ile Gly Leu Gly Asp 
                405                 410                 415     


Tyr Ser Gln Trp Thr Glu Asp Asp Lys Gln Ala Phe Leu Ile Arg Glu 
            420                 425                 430         


Leu Ser Ser Arg Arg Pro Leu Ile Pro His Asn Trp Thr Pro Ser Glu 
        435                 440                 445             


His Thr Arg Glu Ile Leu Asp Thr Cys Lys Val Ile Ala Lys Gln Pro 
    450                 455                 460                 


Glu Gly Val Ile Ser Cys Tyr Ile Ile Ser Met Ala Arg Thr Ala Ser 
465                 470                 475                 480 


Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Ser Tyr 
                485                 490                 495     


His Leu Pro Val Val Pro Leu Phe Glu Thr Leu Asp Asp Leu Asp Ala 
            500                 505                 510         


Ser Lys Glu Val Met Thr Gln Leu Phe Asn Val Gly Trp Tyr Arg Gly 
        515                 520                 525             


Val Ile Lys Asn Arg Gln Met Ile Met Ile Gly Tyr Ser Asp Ser Ala 
    530                 535                 540                 


Lys Asp Ala Gly Met Met Ala Ala Ser Trp Ala Gln Tyr Arg Ala Gln 
545                 550                 555                 560 


Asp Ala Leu Val Lys Leu Cys Glu Gln Thr Gly Ile Glu Leu Thr Leu 
                565                 570                 575     


Phe His Gly Arg Gly Gly Thr Val Gly Arg Gly Gly Ala Pro Ala His 
            580                 585                 590         


Ala Ala Leu Leu Ser Gln Pro Pro Arg Ser Leu Lys Asn Gly Leu Arg 
        595                 600                 605             


Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Leu Gly Leu Pro Ala 
    610                 615                 620                 


Ile Ala Ala Glu Ser Leu Asp Leu Tyr Ala Ser Ala Ile Leu Glu Ala 
625                 630                 635                 640 


Asn Leu Leu Pro Pro Pro Glu Pro Lys Ala Ser Trp Cys Arg Val Met 
                645                 650                 655     


Asp Glu Leu Ala Val Ala Ser Cys Glu Ile Tyr Arg Asn Val Val Arg 
            660                 665                 670         


Gly Asp Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu Gln 
        675                 680                 685             


Glu Leu Ala Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Asn Pro 
    690                 695                 700                 


Asn Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala Trp 
705                 710                 715                 720 


Met Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Ala Ser 
                725                 730                 735     


Ile Arg Gln Ala Met Glu Ser Gly Lys Ala Ala Val Ile Glu Glu Met 
            740                 745                 750         


Cys Asn His Trp Pro Phe Phe Asn Thr Arg Ile Gly Met Leu Glu Met 
        755                 760                 765             


Val Phe Ser Lys Thr Asp Ser Trp Leu Ser Glu Tyr Tyr Asp Gln Arg 
    770                 775                 780                 


Leu Val Lys Lys Glu Leu Trp Tyr Leu Gly Glu Ser Leu Arg Lys Gln 
785                 790                 795                 800 


Leu Ser Glu Asp Ile Ala Thr Val Leu Arg Leu Ser Gly Lys Gly Asp 
                805                 810                 815     


Gln Leu Met Ser Asp Leu Pro Trp Val Ala Glu Ser Ile Ala Leu Arg 
            820                 825                 830         


Asn Val Tyr Thr Asp Pro Leu Asn Leu Leu Gln Val Glu Leu Leu Arg 
        835                 840                 845             


Arg Leu Arg Ala Asp Pro Glu His Pro Asn Pro Asp Ile Glu Gln Ala 
    850                 855                 860                 


Leu Met Ile Thr Ile Thr Gly Ile Ala Ala Gly Met Arg Asn Thr Gly 
865                 870                 875                 880 


<210>  7
<211>  3543
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(3543)

<400>  7
atg tca act gtg gaa gat cac tcc tcc cta cat aaa ttg aga aag gaa         48
Met Ser Thr Val Glu Asp His Ser Ser Leu His Lys Leu Arg Lys Glu           
1               5                   10                  15                

tct gag att ctt tcc aat gca aac aaa atc tta gtg gct aat aga ggt         96
Ser Glu Ile Leu Ser Asn Ala Asn Lys Ile Leu Val Ala Asn Arg Gly           
            20                  25                  30                    

gaa att cca att aga att ttc agg tca gcc cat gaa ttg tca atg cat        144
Glu Ile Pro Ile Arg Ile Phe Arg Ser Ala His Glu Leu Ser Met His           
        35                  40                  45                        

act gtg gcg atc tat tcc cat gaa gat cgg ttg tcc atg cat agg ttg        192
Thr Val Ala Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu           
    50                  55                  60                            

aag gcc gac gag gct tat gca atc ggt aag act ggt caa tat tcg cca        240
Lys Ala Asp Glu Ala Tyr Ala Ile Gly Lys Thr Gly Gln Tyr Ser Pro           
65                  70                  75                  80            

gtt caa gct tat cta caa att gac gaa att atc aaa ata gca aag gaa        288
Val Gln Ala Tyr Leu Gln Ile Asp Glu Ile Ile Lys Ile Ala Lys Glu           
                85                  90                  95                

cat gat gtt tcc atg atc cat cca ggt tat ggt ttc tta tct gaa aac        336
His Asp Val Ser Met Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn           
            100                 105                 110                   

tcc gaa ttc gca aag aag gtt gaa gaa tcc ggt atg att tgg gtt ggg        384
Ser Glu Phe Ala Lys Lys Val Glu Glu Ser Gly Met Ile Trp Val Gly           
        115                 120                 125                       

cct cct gct gaa gtt att gat tct gtt ggt gac aag gtt tct gca aga        432
Pro Pro Ala Glu Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg           
    130                 135                 140                           

aat ttg gca att aaa tgt gac gtt cct gtt gtt cct ggt acc gat ggt        480
Asn Leu Ala Ile Lys Cys Asp Val Pro Val Val Pro Gly Thr Asp Gly           
145                 150                 155                 160           

cca att gaa gac att gaa cag gct aaa cag ttt gtg gaa caa tat ggt        528
Pro Ile Glu Asp Ile Glu Gln Ala Lys Gln Phe Val Glu Gln Tyr Gly           
                165                 170                 175               

tat cct gtc att ata aag gct gca ttt ggt ggt ggt ggt aga ggt atg        576
Tyr Pro Val Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met           
            180                 185                 190                   

aga gtt gtt aga gaa ggt gat gat ata gtt gat gct ttc caa aga gcg        624
Arg Val Val Arg Glu Gly Asp Asp Ile Val Asp Ala Phe Gln Arg Ala           
        195                 200                 205                       

tca tct gaa gca aag tct gcc ttt ggt aat ggt act tgt ttt att gaa        672
Ser Ser Glu Ala Lys Ser Ala Phe Gly Asn Gly Thr Cys Phe Ile Glu           
    210                 215                 220                           

aga ttt ttg gat aag cca aaa cat att gag gtt caa tta ttg gct gat        720
Arg Phe Leu Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp           
225                 230                 235                 240           

aat tat ggt aac aca atc cat ctc ttt gaa aga gat tgt tct gtt caa        768
Asn Tyr Gly Asn Thr Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln           
                245                 250                 255               

aga aga cat caa aag gtt gtt gaa att gca cct gcc aaa act tta cct        816
Arg Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Lys Thr Leu Pro           
            260                 265                 270                   

gtt gaa gtt aga aat gct ata tta aag gat gct gta acg tta gct aaa        864
Val Glu Val Arg Asn Ala Ile Leu Lys Asp Ala Val Thr Leu Ala Lys           
        275                 280                 285                       

acc gct aac tat aga aat gct ggt act gca gaa ttt tta gtt gat tcc        912
Thr Ala Asn Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Ser           
    290                 295                 300                           

caa aac aga cat tat ttt att gaa att aat cca aga att caa gtt gaa        960
Gln Asn Arg His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu           
305                 310                 315                 320           

cat aca att act gaa gaa atc acg ggt gtt gat att gtt gcc gct caa       1008
His Thr Ile Thr Glu Glu Ile Thr Gly Val Asp Ile Val Ala Ala Gln           
                325                 330                 335               

att caa att gct gca ggt gca tca ttg gaa caa ttg ggt cta tta caa       1056
Ile Gln Ile Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln           
            340                 345                 350                   

aac aaa att aca act aga ggt ttt gca att caa tgt aga att aca acc       1104
Asn Lys Ile Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr           
        355                 360                 365                       

gag gat cct gct aag aat ttt gcc cca gat aca ggt aaa att gag gtt       1152
Glu Asp Pro Ala Lys Asn Phe Ala Pro Asp Thr Gly Lys Ile Glu Val           
    370                 375                 380                           

tat aga tct gca ggt ggt aac ggt gtc aga tta gat ggt ggt aat ggg       1200
Tyr Arg Ser Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Gly           
385                 390                 395                 400           

ttt gcc ggt gct gtt ata tct cct cat tat gac tcg atg ttg gtt aaa       1248
Phe Ala Gly Ala Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys           
                405                 410                 415               

tgt tca aca tct ggt tct aac tat gaa att gcc aga aga aag atg att       1296
Cys Ser Thr Ser Gly Ser Asn Tyr Glu Ile Ala Arg Arg Lys Met Ile           
            420                 425                 430                   

aga gct tta gtt gaa ttt aga atc aga ggt gtc aag acc aat att cct       1344
Arg Ala Leu Val Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro           
        435                 440                 445                       

ttc tta ttg gca ttg cta act cat cca gtt ttc att tcg ggt gat tgt       1392
Phe Leu Leu Ala Leu Leu Thr His Pro Val Phe Ile Ser Gly Asp Cys           
    450                 455                 460                           

tgg aca act ttt att gat gat acc cct tcg tta ttc gaa atg gtt tct       1440
Trp Thr Thr Phe Ile Asp Asp Thr Pro Ser Leu Phe Glu Met Val Ser           
465                 470                 475                 480           

tca aag aat aga gcc caa aaa tta ttg gca tat att ggt gac ttg tgt       1488
Ser Lys Asn Arg Ala Gln Lys Leu Leu Ala Tyr Ile Gly Asp Leu Cys           
                485                 490                 495               

gtc aat ggt tct tca att aaa ggt caa att ggt ttc cct aaa ttg aac       1536
Val Asn Gly Ser Ser Ile Lys Gly Gln Ile Gly Phe Pro Lys Leu Asn           
            500                 505                 510                   

aag gaa gca gaa atc cca gat ttg ttg gat cca aat gat gag gtt att       1584
Lys Glu Ala Glu Ile Pro Asp Leu Leu Asp Pro Asn Asp Glu Val Ile           
        515                 520                 525                       

gat gtt tct aaa cct tct acc aat ggt cta aga ccg tat cta tta aag       1632
Asp Val Ser Lys Pro Ser Thr Asn Gly Leu Arg Pro Tyr Leu Leu Lys           
    530                 535                 540                           

tat gga cca gat gcg ttt tcc aaa aaa gtt cgt gaa ttc gat ggt tgt       1680
Tyr Gly Pro Asp Ala Phe Ser Lys Lys Val Arg Glu Phe Asp Gly Cys           
545                 550                 555                 560           

atg att atg gat acc acc tgg aga gat gca cat caa tca tta ttg gct       1728
Met Ile Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala           
                565                 570                 575               

aca aga gtt aga act att gat tta ctg aga att gct cca acg act agt       1776
Thr Arg Val Arg Thr Ile Asp Leu Leu Arg Ile Ala Pro Thr Thr Ser           
            580                 585                 590                   

cat gcc tta caa aat gca ttt gca tta gaa tgt tgg ggt ggc gca aca       1824
His Ala Leu Gln Asn Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr           
        595                 600                 605                       

ttt gat gtt gcg atg agg ttc ctc tat gaa gat cct tgg gag aga tta       1872
Phe Asp Val Ala Met Arg Phe Leu Tyr Glu Asp Pro Trp Glu Arg Leu           
    610                 615                 620                           

aga caa ctt aga aag gca gtt cca aat att cct ttc caa atg tta ttg       1920
Arg Gln Leu Arg Lys Ala Val Pro Asn Ile Pro Phe Gln Met Leu Leu           
625                 630                 635                 640           

aga ggt gct aat ggt gtt gct tat tcg tca tta cct gat aat gca att       1968
Arg Gly Ala Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile           
                645                 650                 655               

gat cat ttt gtt aag caa gca aag gat aat ggt gtt gat att ttc aga       2016
Asp His Phe Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg           
            660                 665                 670                   

gtc ttt gat gct ttg aac gat ttg gaa caa ttg aag gtt ggt gtt gat       2064
Val Phe Asp Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp           
        675                 680                 685                       

gct gtc aag aaa gcc gga ggt gtt gtt gaa gct aca gtt tgt tac tca       2112
Ala Val Lys Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Tyr Ser           
    690                 695                 700                           

ggt gat atg tta att cca ggt aaa aag tat aac ttg gat tat tat tta       2160
Gly Asp Met Leu Ile Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu           
705                 710                 715                 720           

gag act gtt gga aag att gtg gaa atg ggt acc cat att tta ggt att       2208
Glu Thr Val Gly Lys Ile Val Glu Met Gly Thr His Ile Leu Gly Ile           
                725                 730                 735               

aag gat atg gct ggc acg tta aag cca aag gct gct aag ttg ttg att       2256
Lys Asp Met Ala Gly Thr Leu Lys Pro Lys Ala Ala Lys Leu Leu Ile           
            740                 745                 750                   

ggc tcg atc aga tca aaa tac cct gac ttg gtt atc cat gtc cat acc       2304
Gly Ser Ile Arg Ser Lys Tyr Pro Asp Leu Val Ile His Val His Thr           
        755                 760                 765                       

cat gac tct gct ggt acc ggt att tca act tat gtt gca tgc gca ttg       2352
His Asp Ser Ala Gly Thr Gly Ile Ser Thr Tyr Val Ala Cys Ala Leu           
    770                 775                 780                           

gca ggt gcc gac att gtc gat tgt gca atc aat tcg atg tct ggt tta       2400
Ala Gly Ala Asp Ile Val Asp Cys Ala Ile Asn Ser Met Ser Gly Leu           
785                 790                 795                 800           

acc tct caa cct tca atg agt gct ttt att gct gct tta gat ggt gat       2448
Thr Ser Gln Pro Ser Met Ser Ala Phe Ile Ala Ala Leu Asp Gly Asp           
                805                 810                 815               

atc gaa act ggt gtt cca gaa cat ttt gca aga caa tta gat gca tac       2496
Ile Glu Thr Gly Val Pro Glu His Phe Ala Arg Gln Leu Asp Ala Tyr           
            820                 825                 830                   

tgg gca gaa atg aga ttg tta tac tca tgt ttc gaa gcc gac ttg aag       2544
Trp Ala Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys           
        835                 840                 845                       

gga cca gac cca gaa gtt tat aaa cat gaa att cca ggt gga cag ttg       2592
Gly Pro Asp Pro Glu Val Tyr Lys His Glu Ile Pro Gly Gly Gln Leu           
    850                 855                 860                           

act aac cta atc ttc caa gcc caa caa gtt ggt ttg ggt gaa caa tgg       2640
Thr Asn Leu Ile Phe Gln Ala Gln Gln Val Gly Leu Gly Glu Gln Trp           
865                 870                 875                 880           

gaa gaa act aag aag aag tat gaa gat gct aac atg ttg ttg ggt gat       2688
Glu Glu Thr Lys Lys Lys Tyr Glu Asp Ala Asn Met Leu Leu Gly Asp           
                885                 890                 895               

att gtc aag gtt acc cca acc tcc aag gtt gtt ggt gat tta gcc caa       2736
Ile Val Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln           
            900                 905                 910                   

ttt atg gtt tct aat aaa tta gaa aaa gaa gat gtt gaa aaa ctt gct       2784
Phe Met Val Ser Asn Lys Leu Glu Lys Glu Asp Val Glu Lys Leu Ala           
        915                 920                 925                       

aat gaa tta gat ttc cca gat tca gtt ctt gat ttc ttt gaa gga tta       2832
Asn Glu Leu Asp Phe Pro Asp Ser Val Leu Asp Phe Phe Glu Gly Leu           
    930                 935                 940                           

atg ggt aca cca tat ggt gga ttc cca gag cct ttg aga aca aat gtc       2880
Met Gly Thr Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Thr Asn Val           
945                 950                 955                 960           

att tcc ggc aag aga aga aaa tta aag ggt aga cca ggt tta gaa tta       2928
Ile Ser Gly Lys Arg Arg Lys Leu Lys Gly Arg Pro Gly Leu Glu Leu           
                965                 970                 975               

gaa cct ttc aac ctc gag gaa atc aga gaa aat ttg gtt tcc aga ttt       2976
Glu Pro Phe Asn Leu Glu Glu Ile Arg Glu Asn Leu Val Ser Arg Phe           
            980                 985                 990                   

ggt cca ggt att act gaa tgt gat  gtt gca tct tat aac  atg tat cca     3024
Gly Pro Gly Ile Thr Glu Cys Asp  Val Ala Ser Tyr Asn  Met Tyr Pro         
        995                 1000                 1005                     

aag gtt  tac gag caa tat cgt  aag gtg gtt gaa aaa  tat ggt gat        3069
Lys Val  Tyr Glu Gln Tyr Arg  Lys Val Val Glu Lys  Tyr Gly Asp            
    1010                 1015                 1020                        

tta tct  gtt tta cca aca aaa  gca ttt ttg gct cct  cca act att        3114
Leu Ser  Val Leu Pro Thr Lys  Ala Phe Leu Ala Pro  Pro Thr Ile            
    1025                 1030                 1035                        

ggt gaa  gaa gtt cat gtg gaa  att gag caa ggt aag  act ttg att        3159
Gly Glu  Glu Val His Val Glu  Ile Glu Gln Gly Lys  Thr Leu Ile            
    1040                 1045                 1050                        

att aag  tta tta gcc att tct  gac ttg tct aaa tct  cat ggt aca        3204
Ile Lys  Leu Leu Ala Ile Ser  Asp Leu Ser Lys Ser  His Gly Thr            
    1055                 1060                 1065                        

aga gaa  gta tac ttt gaa ttg  aat ggt gaa atg aga  aag gtt aca        3249
Arg Glu  Val Tyr Phe Glu Leu  Asn Gly Glu Met Arg  Lys Val Thr            
    1070                 1075                 1080                        

att gaa  gat aaa aca gct gca  att gag act gtt aca  aga gca aag        3294
Ile Glu  Asp Lys Thr Ala Ala  Ile Glu Thr Val Thr  Arg Ala Lys            
    1085                 1090                 1095                        

gct gac  gga cac aat cca aat  gaa gtt ggt gcg cca  atg gct ggt        3339
Ala Asp  Gly His Asn Pro Asn  Glu Val Gly Ala Pro  Met Ala Gly            
    1100                 1105                 1110                        

gtc gtt  gtt gaa gtt aga gtg  aag cat gga aca gaa  gtt aag aag        3384
Val Val  Val Glu Val Arg Val  Lys His Gly Thr Glu  Val Lys Lys            
    1115                 1120                 1125                        

ggt gat  cca tta gcc gtt ttg  agt gca atg aaa atg  gaa atg gtt        3429
Gly Asp  Pro Leu Ala Val Leu  Ser Ala Met Lys Met  Glu Met Val            
    1130                 1135                 1140                        

att tct  gct cct gtt agt ggt  agg gtc ggt gaa gtt  ttt gtc aac        3474
Ile Ser  Ala Pro Val Ser Gly  Arg Val Gly Glu Val  Phe Val Asn            
    1145                 1150                 1155                        

gaa ggc  gat tcc gtt gat atg  ggt gat ttg ctt gtg  aaa att gcc        3519
Glu Gly  Asp Ser Val Asp Met  Gly Asp Leu Leu Val  Lys Ile Ala            
    1160                 1165                 1170                        

aaa gat  gaa gcg cca gca gct  taa                                     3543
Lys Asp  Glu Ala Pro Ala Ala                                              
    1175                 1180                                             


<210>  8
<211>  1180
<212>  PRT
<213>  Issatchenkia orientalis

<400>  8

Met Ser Thr Val Glu Asp His Ser Ser Leu His Lys Leu Arg Lys Glu 
1               5                   10                  15      


Ser Glu Ile Leu Ser Asn Ala Asn Lys Ile Leu Val Ala Asn Arg Gly 
            20                  25                  30          


Glu Ile Pro Ile Arg Ile Phe Arg Ser Ala His Glu Leu Ser Met His 
        35                  40                  45              


Thr Val Ala Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu 
    50                  55                  60                  


Lys Ala Asp Glu Ala Tyr Ala Ile Gly Lys Thr Gly Gln Tyr Ser Pro 
65                  70                  75                  80  


Val Gln Ala Tyr Leu Gln Ile Asp Glu Ile Ile Lys Ile Ala Lys Glu 
                85                  90                  95      


His Asp Val Ser Met Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn 
            100                 105                 110         


Ser Glu Phe Ala Lys Lys Val Glu Glu Ser Gly Met Ile Trp Val Gly 
        115                 120                 125             


Pro Pro Ala Glu Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg 
    130                 135                 140                 


Asn Leu Ala Ile Lys Cys Asp Val Pro Val Val Pro Gly Thr Asp Gly 
145                 150                 155                 160 


Pro Ile Glu Asp Ile Glu Gln Ala Lys Gln Phe Val Glu Gln Tyr Gly 
                165                 170                 175     


Tyr Pro Val Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met 
            180                 185                 190         


Arg Val Val Arg Glu Gly Asp Asp Ile Val Asp Ala Phe Gln Arg Ala 
        195                 200                 205             


Ser Ser Glu Ala Lys Ser Ala Phe Gly Asn Gly Thr Cys Phe Ile Glu 
    210                 215                 220                 


Arg Phe Leu Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp 
225                 230                 235                 240 


Asn Tyr Gly Asn Thr Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln 
                245                 250                 255     


Arg Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Lys Thr Leu Pro 
            260                 265                 270         


Val Glu Val Arg Asn Ala Ile Leu Lys Asp Ala Val Thr Leu Ala Lys 
        275                 280                 285             


Thr Ala Asn Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Ser 
    290                 295                 300                 


Gln Asn Arg His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu 
305                 310                 315                 320 


His Thr Ile Thr Glu Glu Ile Thr Gly Val Asp Ile Val Ala Ala Gln 
                325                 330                 335     


Ile Gln Ile Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln 
            340                 345                 350         


Asn Lys Ile Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr 
        355                 360                 365             


Glu Asp Pro Ala Lys Asn Phe Ala Pro Asp Thr Gly Lys Ile Glu Val 
    370                 375                 380                 


Tyr Arg Ser Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Gly 
385                 390                 395                 400 


Phe Ala Gly Ala Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys 
                405                 410                 415     


Cys Ser Thr Ser Gly Ser Asn Tyr Glu Ile Ala Arg Arg Lys Met Ile 
            420                 425                 430         


Arg Ala Leu Val Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro 
        435                 440                 445             


Phe Leu Leu Ala Leu Leu Thr His Pro Val Phe Ile Ser Gly Asp Cys 
    450                 455                 460                 


Trp Thr Thr Phe Ile Asp Asp Thr Pro Ser Leu Phe Glu Met Val Ser 
465                 470                 475                 480 


Ser Lys Asn Arg Ala Gln Lys Leu Leu Ala Tyr Ile Gly Asp Leu Cys 
                485                 490                 495     


Val Asn Gly Ser Ser Ile Lys Gly Gln Ile Gly Phe Pro Lys Leu Asn 
            500                 505                 510         


Lys Glu Ala Glu Ile Pro Asp Leu Leu Asp Pro Asn Asp Glu Val Ile 
        515                 520                 525             


Asp Val Ser Lys Pro Ser Thr Asn Gly Leu Arg Pro Tyr Leu Leu Lys 
    530                 535                 540                 


Tyr Gly Pro Asp Ala Phe Ser Lys Lys Val Arg Glu Phe Asp Gly Cys 
545                 550                 555                 560 


Met Ile Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala 
                565                 570                 575     


Thr Arg Val Arg Thr Ile Asp Leu Leu Arg Ile Ala Pro Thr Thr Ser 
            580                 585                 590         


His Ala Leu Gln Asn Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr 
        595                 600                 605             


Phe Asp Val Ala Met Arg Phe Leu Tyr Glu Asp Pro Trp Glu Arg Leu 
    610                 615                 620                 


Arg Gln Leu Arg Lys Ala Val Pro Asn Ile Pro Phe Gln Met Leu Leu 
625                 630                 635                 640 


Arg Gly Ala Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile 
                645                 650                 655     


Asp His Phe Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg 
            660                 665                 670         


Val Phe Asp Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp 
        675                 680                 685             


Ala Val Lys Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Tyr Ser 
    690                 695                 700                 


Gly Asp Met Leu Ile Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu 
705                 710                 715                 720 


Glu Thr Val Gly Lys Ile Val Glu Met Gly Thr His Ile Leu Gly Ile 
                725                 730                 735     


Lys Asp Met Ala Gly Thr Leu Lys Pro Lys Ala Ala Lys Leu Leu Ile 
            740                 745                 750         


Gly Ser Ile Arg Ser Lys Tyr Pro Asp Leu Val Ile His Val His Thr 
        755                 760                 765             


His Asp Ser Ala Gly Thr Gly Ile Ser Thr Tyr Val Ala Cys Ala Leu 
    770                 775                 780                 


Ala Gly Ala Asp Ile Val Asp Cys Ala Ile Asn Ser Met Ser Gly Leu 
785                 790                 795                 800 


Thr Ser Gln Pro Ser Met Ser Ala Phe Ile Ala Ala Leu Asp Gly Asp 
                805                 810                 815     


Ile Glu Thr Gly Val Pro Glu His Phe Ala Arg Gln Leu Asp Ala Tyr 
            820                 825                 830         


Trp Ala Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys 
        835                 840                 845             


Gly Pro Asp Pro Glu Val Tyr Lys His Glu Ile Pro Gly Gly Gln Leu 
    850                 855                 860                 


Thr Asn Leu Ile Phe Gln Ala Gln Gln Val Gly Leu Gly Glu Gln Trp 
865                 870                 875                 880 


Glu Glu Thr Lys Lys Lys Tyr Glu Asp Ala Asn Met Leu Leu Gly Asp 
                885                 890                 895     


Ile Val Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln 
            900                 905                 910         


Phe Met Val Ser Asn Lys Leu Glu Lys Glu Asp Val Glu Lys Leu Ala 
        915                 920                 925             


Asn Glu Leu Asp Phe Pro Asp Ser Val Leu Asp Phe Phe Glu Gly Leu 
    930                 935                 940                 


Met Gly Thr Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Thr Asn Val 
945                 950                 955                 960 


Ile Ser Gly Lys Arg Arg Lys Leu Lys Gly Arg Pro Gly Leu Glu Leu 
                965                 970                 975     


Glu Pro Phe Asn Leu Glu Glu Ile Arg Glu Asn Leu Val Ser Arg Phe 
            980                 985                 990         


Gly Pro Gly Ile Thr Glu Cys Asp  Val Ala Ser Tyr Asn  Met Tyr Pro 
        995                 1000                 1005             


Lys Val  Tyr Glu Gln Tyr Arg  Lys Val Val Glu Lys  Tyr Gly Asp 
    1010                 1015                 1020             


Leu Ser  Val Leu Pro Thr Lys  Ala Phe Leu Ala Pro  Pro Thr Ile 
    1025                 1030                 1035             


Gly Glu  Glu Val His Val Glu  Ile Glu Gln Gly Lys  Thr Leu Ile 
    1040                 1045                 1050             


Ile Lys  Leu Leu Ala Ile Ser  Asp Leu Ser Lys Ser  His Gly Thr 
    1055                 1060                 1065             


Arg Glu  Val Tyr Phe Glu Leu  Asn Gly Glu Met Arg  Lys Val Thr 
    1070                 1075                 1080             


Ile Glu  Asp Lys Thr Ala Ala  Ile Glu Thr Val Thr  Arg Ala Lys 
    1085                 1090                 1095             


Ala Asp  Gly His Asn Pro Asn  Glu Val Gly Ala Pro  Met Ala Gly 
    1100                 1105                 1110             


Val Val  Val Glu Val Arg Val  Lys His Gly Thr Glu  Val Lys Lys 
    1115                 1120                 1125             


Gly Asp  Pro Leu Ala Val Leu  Ser Ala Met Lys Met  Glu Met Val 
    1130                 1135                 1140             


Ile Ser  Ala Pro Val Ser Gly  Arg Val Gly Glu Val  Phe Val Asn 
    1145                 1150                 1155             


Glu Gly  Asp Ser Val Asp Met  Gly Asp Leu Leu Val  Lys Ile Ala 
    1160                 1165                 1170             


Lys Asp  Glu Ala Pro Ala Ala  
    1175                 1180 


<210>  9
<211>  3537
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(3537)

<400>  9
atg tcg caa aga aaa ttc gcc ggc ttg aga gat aac ttc aat ctc ttg         48
Met Ser Gln Arg Lys Phe Ala Gly Leu Arg Asp Asn Phe Asn Leu Leu           
1               5                   10                  15                

ggt gaa aag aac aaa ata ttg gtg gct aat aga gga gaa att cca atc         96
Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro Ile           
            20                  25                  30                    

aga att ttt cgt acc gct cat gaa ctg tct atg cag acg gta gct ata        144
Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Gln Thr Val Ala Ile           
        35                  40                  45                        

tat tct cat gaa gat cgt ctt tca acg cac aaa caa aag gct gac gaa        192
Tyr Ser His Glu Asp Arg Leu Ser Thr His Lys Gln Lys Ala Asp Glu           
    50                  55                  60                            

gca tac gtc ata ggt gaa gta ggc caa tat acc ccc gtc ggc gct tat        240
Ala Tyr Val Ile Gly Glu Val Gly Gln Tyr Thr Pro Val Gly Ala Tyr           
65                  70                  75                  80            

ttg gcc att gac gaa atc att tcc att gcc caa aaa cac cag gta gat        288
Leu Ala Ile Asp Glu Ile Ile Ser Ile Ala Gln Lys His Gln Val Asp           
                85                  90                  95                

ttc atc cat cca ggt tat ggg ttc ttg tct gaa aat tcg gaa ttt gcc        336
Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe Ala           
            100                 105                 110                   

gac aaa gta gtg aag gcc ggt atc act tgg att ggc cct cca gct gaa        384
Asp Lys Val Val Lys Ala Gly Ile Thr Trp Ile Gly Pro Pro Ala Glu           
        115                 120                 125                       

gtt att gac tcc gtg ggt gat aag gtc tca gct aga aac ctg gca gca        432
Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala Ala           
    130                 135                 140                           

aaa gct aat gtg ccc acc gtt cct ggt aca cca ggt cct ata gaa act        480
Lys Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro Ile Glu Thr           
145                 150                 155                 160           

gta gag gaa gca ctt gac ttc gtc aat gaa tac ggc tac ccg gtg atc        528
Val Glu Glu Ala Leu Asp Phe Val Asn Glu Tyr Gly Tyr Pro Val Ile           
                165                 170                 175               

att aag gcc gcc ttt ggt ggt ggt ggt aga ggt atg aga gtc gtt aga        576
Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val Arg           
            180                 185                 190                   

gaa ggt gac gac gtg gca gat gcc ttt caa cgt gct acc tcc gaa gcc        624
Glu Gly Asp Asp Val Ala Asp Ala Phe Gln Arg Ala Thr Ser Glu Ala           
        195                 200                 205                       

cgt act gcc ttc ggt aat ggt acc tgc ttt gtg gaa aga ttc ttg gac        672
Arg Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu Asp           
    210                 215                 220                           

aag cca aag cat att gaa gtt caa ttg ttg gcc gat aac cac gga aac        720
Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn His Gly Asn           
225                 230                 235                 240           

gtg gtt cat ctt ttc gaa aga gac tgt tcc gtg cag aga aga cac caa        768
Val Val His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His Gln           
                245                 250                 255               

aag gtt gtc gaa gtg gcc cca gca aag act tta ccc cgt gaa gtc cgt        816
Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Arg Glu Val Arg           
            260                 265                 270                   

gac gcc att ttg aca gat gca gtt aaa ttg gcc aaa gag tgt ggc tac        864
Asp Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Cys Gly Tyr           
        275                 280                 285                       

aga aat gcg ggt act gct gaa ttc ttg gtt gat aac caa aat aga cac        912
Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg His           
    290                 295                 300                           

tat ttc att gaa att aat cca aga atc caa gtg gaa cat acc atc aca        960
Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile Thr           
305                 310                 315                 320           

gaa gaa att acc ggt ata gat att gtg gcg gct cag atc caa att gcg       1008
Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile Ala           
                325                 330                 335               

gca ggt gcc tct cta ccc cag ctg ggc cta ttc cag gac aaa att acg       1056
Ala Gly Ala Ser Leu Pro Gln Leu Gly Leu Phe Gln Asp Lys Ile Thr           
            340                 345                 350                   

act cgt ggc ttt gcc att cag tgc cgt att acc acg gaa gac cct gct       1104
Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro Ala           
        355                 360                 365                       

aag aac ttc caa cca gat acc ggt aga ata gaa gtg tac cgt tct gca       1152
Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Glu Val Tyr Arg Ser Ala           
    370                 375                 380                           

ggt ggt aat ggt gtt aga ctg gat ggt ggt aac gcc tat gca gga aca       1200
Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Tyr Ala Gly Thr           
385                 390                 395                 400           

ata atc tca cct cat tac gac tca atg ctg gtc aaa tgc tca tgc tcc       1248
Ile Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys Ser           
                405                 410                 415               

ggt tcc acc tac gaa atc gtt cgt aga aaa atg att cgt gca tta atc       1296
Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Ile Arg Ala Leu Ile           
            420                 425                 430                   

gag ttc aga att aga ggt gtc aag acc aac att ccc ttc cta ttg act       1344
Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu Thr           
        435                 440                 445                       

ctt ttg acc aat cca gta ttt att gag ggt aca tac tgg acg act ttt       1392
Leu Leu Thr Asn Pro Val Phe Ile Glu Gly Thr Tyr Trp Thr Thr Phe           
    450                 455                 460                           

att gac gac acc cca caa ctg ttc caa atg gtt tca tca caa aac aga       1440
Ile Asp Asp Thr Pro Gln Leu Phe Gln Met Val Ser Ser Gln Asn Arg           
465                 470                 475                 480           

gcc caa aaa ctt tta cat tac ctc gcc gac gtg gca gtc aat ggt tca       1488
Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Val Ala Val Asn Gly Ser           
                485                 490                 495               

tct atc aag ggt caa att ggc ttg cca aaa tta aaa tca aat cca agt       1536
Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Ser Asn Pro Ser           
            500                 505                 510                   

gtc ccc cat ttg cac gat gct cag ggc aat gtc atc aac gtt aca aag       1584
Val Pro His Leu His Asp Ala Gln Gly Asn Val Ile Asn Val Thr Lys           
        515                 520                 525                       

tct gca cca cca tcc gga tgg agg caa gtg cta cta gaa aag ggg cca       1632
Ser Ala Pro Pro Ser Gly Trp Arg Gln Val Leu Leu Glu Lys Gly Pro           
    530                 535                 540                           

gct gaa ttt gcc aga caa gtt aga cag ttc aat ggt act tta ttg atg       1680
Ala Glu Phe Ala Arg Gln Val Arg Gln Phe Asn Gly Thr Leu Leu Met           
545                 550                 555                 560           

gac acc acc tgg aga gac gct cat caa tct cta ctt gca aca aga gtc       1728
Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val           
                565                 570                 575               

aga acc cac gat ttg gct aca atc gct cca aca acc gca cat gcc ctt       1776
Arg Thr His Asp Leu Ala Thr Ile Ala Pro Thr Thr Ala His Ala Leu           
            580                 585                 590                   

gca ggt cgt ttc gcc tta gaa tgt tgg ggt ggt gcc aca ttc gat gtt       1824
Ala Gly Arg Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val           
        595                 600                 605                       

gca atg aga ttt ttg cat gag gat cca tgg gaa cgt ttg aga aaa tta       1872
Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Lys Leu           
    610                 615                 620                           

aga tct ctg gtg cct aat att cca ttc caa atg tta ttg cgt ggt gcc       1920
Arg Ser Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala           
625                 630                 635                 640           

aat ggt gtg gct tat tct tca ttg cct gac aat gct att gac cat ttc       1968
Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe           
                645                 650                 655               

gtc aag caa gcc aag gat aat ggt gtt gat ata ttt aga gtc ttt gat       2016
Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp           
            660                 665                 670                   

gcc tta aat gac ttg gaa caa ttg aag gtc ggt gta gat gct gtg aag       2064
Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp Ala Val Lys           
        675                 680                 685                       

aag gca ggt ggt gtt gta gaa gcc act gtt tgt ttc tct ggg gat atg       2112
Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Phe Ser Gly Asp Met           
    690                 695                 700                           

ctt cag cca ggc aag aaa tac aat ttg gat tac tac ttg gaa att gct       2160
Leu Gln Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Glu Ile Ala           
705                 710                 715                 720           

gaa aaa att gtc caa atg ggc act cat atc ctg ggt atc aaa gat atg       2208
Glu Lys Ile Val Gln Met Gly Thr His Ile Leu Gly Ile Lys Asp Met           
                725                 730                 735               

gca ggt acc atg aag cca gca gct gcc aaa cta ctg att gga tct ttg       2256
Ala Gly Thr Met Lys Pro Ala Ala Ala Lys Leu Leu Ile Gly Ser Leu           
            740                 745                 750                   

agg gct aag tac cct gat ctc cca ata cat gtt cac act cac gat tct       2304
Arg Ala Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser           
        755                 760                 765                       

gca ggt act gct gtt gca tca atg act gcg tgt gct ctg gcg ggc gcc       2352
Ala Gly Thr Ala Val Ala Ser Met Thr Ala Cys Ala Leu Ala Gly Ala           
    770                 775                 780                           

gat gtc gtt gat gtt gcc atc aac tca atg tct ggt tta act tca caa       2400
Asp Val Val Asp Val Ala Ile Asn Ser Met Ser Gly Leu Thr Ser Gln           
785                 790                 795                 800           

cca tca atc aat gct ctg ttg gct tca tta gaa ggt aat att gac act       2448
Pro Ser Ile Asn Ala Leu Leu Ala Ser Leu Glu Gly Asn Ile Asp Thr           
                805                 810                 815               

ggt att aac gtt gag cat gtc cgt gaa cta gat gca tat tgg gca gag       2496
Gly Ile Asn Val Glu His Val Arg Glu Leu Asp Ala Tyr Trp Ala Glu           
            820                 825                 830                   

atg aga ttg tta tac tct tgt ttc gag gct gac ttg aag ggc cca gat       2544
Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp           
        835                 840                 845                       

cca gaa gtt tat caa cat gaa atc cca ggt ggt caa ttg aca aac ttg       2592
Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu           
    850                 855                 860                           

ttg ttt caa gcc caa caa ttg ggt ctt gga gaa caa tgg gcc gaa aca       2640
Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr           
865                 870                 875                 880           

aaa aga gct tac aga gaa gcc aat tat tta ttg ggt gat att gtc aaa       2688
Lys Arg Ala Tyr Arg Glu Ala Asn Tyr Leu Leu Gly Asp Ile Val Lys           
                885                 890                 895               

gtt acc cca act tcg aag gtc gtt ggt gat ctg gca caa ttt atg gtc       2736
Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val           
            900                 905                 910                   

tcc aat aaa tta act tcc gat gat gtg aga cgc ctg gct aat tct ttg       2784
Ser Asn Lys Leu Thr Ser Asp Asp Val Arg Arg Leu Ala Asn Ser Leu           
        915                 920                 925                       

gat ttc cct gac tct gtt atg gat ttc ttc gaa ggc tta atc ggc caa       2832
Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln           
    930                 935                 940                           

cca tac ggt ggg ttc cca gaa cca ttt aga tca gac gtt tta agg aac       2880
Pro Tyr Gly Gly Phe Pro Glu Pro Phe Arg Ser Asp Val Leu Arg Asn           
945                 950                 955                 960           

aag aga aga aag ttg act tgt cgt cca ggc ctg gaa cta gag cca ttt       2928
Lys Arg Arg Lys Leu Thr Cys Arg Pro Gly Leu Glu Leu Glu Pro Phe           
                965                 970                 975               

gat ctc gaa aaa att aga gaa gac ttg cag aat aga ttt ggt gat gtt       2976
Asp Leu Glu Lys Ile Arg Glu Asp Leu Gln Asn Arg Phe Gly Asp Val           
            980                 985                 990                   

gat gag tgc gac gtt gct tct tat  aac atg tac cca aga  gtt tat gaa     3024
Asp Glu Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Arg  Val Tyr Glu         
        995                 1000                 1005                     

gac ttc  caa aag atg aga gaa  acg tat ggt gat tta  tct gta ttg        3069
Asp Phe  Gln Lys Met Arg Glu  Thr Tyr Gly Asp Leu  Ser Val Leu            
    1010                 1015                 1020                        

cca aca  aga agc ttt ttg tct  cca cta gag act gac  gaa gaa att        3114
Pro Thr  Arg Ser Phe Leu Ser  Pro Leu Glu Thr Asp  Glu Glu Ile            
    1025                 1030                 1035                        

gaa gtt  gta atc gaa caa ggt  aaa acg cta att atc  aag cta cag        3159
Glu Val  Val Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Leu Gln            
    1040                 1045                 1050                        

gct gtg  ggt gat ttg aac aaa  aag acc ggt gaa aga  gaa gtt tac        3204
Ala Val  Gly Asp Leu Asn Lys  Lys Thr Gly Glu Arg  Glu Val Tyr            
    1055                 1060                 1065                        

ttt gat  ttg aat ggt gaa atg  aga aaa att cgt gtt  gct gac aga        3249
Phe Asp  Leu Asn Gly Glu Met  Arg Lys Ile Arg Val  Ala Asp Arg            
    1070                 1075                 1080                        

tca caa  aaa gtg gaa act gtt  act aaa tcc aaa gca  gac atg cat        3294
Ser Gln  Lys Val Glu Thr Val  Thr Lys Ser Lys Ala  Asp Met His            
    1085                 1090                 1095                        

gat cca  tta cac att ggt gca  cca atg gca ggt gtc  att gtt gaa        3339
Asp Pro  Leu His Ile Gly Ala  Pro Met Ala Gly Val  Ile Val Glu            
    1100                 1105                 1110                        

gtt aaa  gtt cat aaa gga tca  cta ata aag aag ggc  caa cct gta        3384
Val Lys  Val His Lys Gly Ser  Leu Ile Lys Lys Gly  Gln Pro Val            
    1115                 1120                 1125                        

gcc gta  tta agc gcc atg aaa  atg gaa atg att ata  tct tct cca        3429
Ala Val  Leu Ser Ala Met Lys  Met Glu Met Ile Ile  Ser Ser Pro            
    1130                 1135                 1140                        

tcc gat  gga caa gtt aaa gaa  gtg ttt gtc tct gat  ggt gaa aat        3474
Ser Asp  Gly Gln Val Lys Glu  Val Phe Val Ser Asp  Gly Glu Asn            
    1145                 1150                 1155                        

gtg gac  tct tct gat tta tta  gtt cta tta gaa gac  caa gtt cct        3519
Val Asp  Ser Ser Asp Leu Leu  Val Leu Leu Glu Asp  Gln Val Pro            
    1160                 1165                 1170                        

gtt gaa  act aag gca taa                                              3537
Val Glu  Thr Lys Ala                                                      
    1175                                                                  


<210>  10
<211>  1178
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  10

Met Ser Gln Arg Lys Phe Ala Gly Leu Arg Asp Asn Phe Asn Leu Leu 
1               5                   10                  15      


Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro Ile 
            20                  25                  30          


Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Gln Thr Val Ala Ile 
        35                  40                  45              


Tyr Ser His Glu Asp Arg Leu Ser Thr His Lys Gln Lys Ala Asp Glu 
    50                  55                  60                  


Ala Tyr Val Ile Gly Glu Val Gly Gln Tyr Thr Pro Val Gly Ala Tyr 
65                  70                  75                  80  


Leu Ala Ile Asp Glu Ile Ile Ser Ile Ala Gln Lys His Gln Val Asp 
                85                  90                  95      


Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe Ala 
            100                 105                 110         


Asp Lys Val Val Lys Ala Gly Ile Thr Trp Ile Gly Pro Pro Ala Glu 
        115                 120                 125             


Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala Ala 
    130                 135                 140                 


Lys Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro Ile Glu Thr 
145                 150                 155                 160 


Val Glu Glu Ala Leu Asp Phe Val Asn Glu Tyr Gly Tyr Pro Val Ile 
                165                 170                 175     


Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val Arg 
            180                 185                 190         


Glu Gly Asp Asp Val Ala Asp Ala Phe Gln Arg Ala Thr Ser Glu Ala 
        195                 200                 205             


Arg Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu Asp 
    210                 215                 220                 


Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn His Gly Asn 
225                 230                 235                 240 


Val Val His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His Gln 
                245                 250                 255     


Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Arg Glu Val Arg 
            260                 265                 270         


Asp Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Cys Gly Tyr 
        275                 280                 285             


Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg His 
    290                 295                 300                 


Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile Thr 
305                 310                 315                 320 


Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile Ala 
                325                 330                 335     


Ala Gly Ala Ser Leu Pro Gln Leu Gly Leu Phe Gln Asp Lys Ile Thr 
            340                 345                 350         


Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro Ala 
        355                 360                 365             


Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Glu Val Tyr Arg Ser Ala 
    370                 375                 380                 


Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Tyr Ala Gly Thr 
385                 390                 395                 400 


Ile Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys Ser 
                405                 410                 415     


Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Ile Arg Ala Leu Ile 
            420                 425                 430         


Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu Thr 
        435                 440                 445             


Leu Leu Thr Asn Pro Val Phe Ile Glu Gly Thr Tyr Trp Thr Thr Phe 
    450                 455                 460                 


Ile Asp Asp Thr Pro Gln Leu Phe Gln Met Val Ser Ser Gln Asn Arg 
465                 470                 475                 480 


Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Val Ala Val Asn Gly Ser 
                485                 490                 495     


Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Ser Asn Pro Ser 
            500                 505                 510         


Val Pro His Leu His Asp Ala Gln Gly Asn Val Ile Asn Val Thr Lys 
        515                 520                 525             


Ser Ala Pro Pro Ser Gly Trp Arg Gln Val Leu Leu Glu Lys Gly Pro 
    530                 535                 540                 


Ala Glu Phe Ala Arg Gln Val Arg Gln Phe Asn Gly Thr Leu Leu Met 
545                 550                 555                 560 


Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val 
                565                 570                 575     


Arg Thr His Asp Leu Ala Thr Ile Ala Pro Thr Thr Ala His Ala Leu 
            580                 585                 590         


Ala Gly Arg Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val 
        595                 600                 605             


Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Lys Leu 
    610                 615                 620                 


Arg Ser Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala 
625                 630                 635                 640 


Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe 
                645                 650                 655     


Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp 
            660                 665                 670         


Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp Ala Val Lys 
        675                 680                 685             


Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Phe Ser Gly Asp Met 
    690                 695                 700                 


Leu Gln Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Glu Ile Ala 
705                 710                 715                 720 


Glu Lys Ile Val Gln Met Gly Thr His Ile Leu Gly Ile Lys Asp Met 
                725                 730                 735     


Ala Gly Thr Met Lys Pro Ala Ala Ala Lys Leu Leu Ile Gly Ser Leu 
            740                 745                 750         


Arg Ala Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser 
        755                 760                 765             


Ala Gly Thr Ala Val Ala Ser Met Thr Ala Cys Ala Leu Ala Gly Ala 
    770                 775                 780                 


Asp Val Val Asp Val Ala Ile Asn Ser Met Ser Gly Leu Thr Ser Gln 
785                 790                 795                 800 


Pro Ser Ile Asn Ala Leu Leu Ala Ser Leu Glu Gly Asn Ile Asp Thr 
                805                 810                 815     


Gly Ile Asn Val Glu His Val Arg Glu Leu Asp Ala Tyr Trp Ala Glu 
            820                 825                 830         


Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp 
        835                 840                 845             


Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu 
    850                 855                 860                 


Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr 
865                 870                 875                 880 


Lys Arg Ala Tyr Arg Glu Ala Asn Tyr Leu Leu Gly Asp Ile Val Lys 
                885                 890                 895     


Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val 
            900                 905                 910         


Ser Asn Lys Leu Thr Ser Asp Asp Val Arg Arg Leu Ala Asn Ser Leu 
        915                 920                 925             


Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln 
    930                 935                 940                 


Pro Tyr Gly Gly Phe Pro Glu Pro Phe Arg Ser Asp Val Leu Arg Asn 
945                 950                 955                 960 


Lys Arg Arg Lys Leu Thr Cys Arg Pro Gly Leu Glu Leu Glu Pro Phe 
                965                 970                 975     


Asp Leu Glu Lys Ile Arg Glu Asp Leu Gln Asn Arg Phe Gly Asp Val 
            980                 985                 990         


Asp Glu Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Arg  Val Tyr Glu 
        995                 1000                 1005             


Asp Phe  Gln Lys Met Arg Glu  Thr Tyr Gly Asp Leu  Ser Val Leu 
    1010                 1015                 1020             


Pro Thr  Arg Ser Phe Leu Ser  Pro Leu Glu Thr Asp  Glu Glu Ile 
    1025                 1030                 1035             


Glu Val  Val Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Leu Gln 
    1040                 1045                 1050             


Ala Val  Gly Asp Leu Asn Lys  Lys Thr Gly Glu Arg  Glu Val Tyr 
    1055                 1060                 1065             


Phe Asp  Leu Asn Gly Glu Met  Arg Lys Ile Arg Val  Ala Asp Arg 
    1070                 1075                 1080             


Ser Gln  Lys Val Glu Thr Val  Thr Lys Ser Lys Ala  Asp Met His 
    1085                 1090                 1095             


Asp Pro  Leu His Ile Gly Ala  Pro Met Ala Gly Val  Ile Val Glu 
    1100                 1105                 1110             


Val Lys  Val His Lys Gly Ser  Leu Ile Lys Lys Gly  Gln Pro Val 
    1115                 1120                 1125             


Ala Val  Leu Ser Ala Met Lys  Met Glu Met Ile Ile  Ser Ser Pro 
    1130                 1135                 1140             


Ser Asp  Gly Gln Val Lys Glu  Val Phe Val Ser Asp  Gly Glu Asn 
    1145                 1150                 1155             


Val Asp  Ser Ser Asp Leu Leu  Val Leu Leu Glu Asp  Gln Val Pro 
    1160                 1165                 1170             


Val Glu  Thr Lys Ala 
    1175             


<210>  11
<211>  3528
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(3528)

<400>  11
atg tct acc caa aac gat ctg gcc ggg ttg cgt gat aac tcg aac cta         48
Met Ser Thr Gln Asn Asp Leu Ala Gly Leu Arg Asp Asn Ser Asn Leu           
1               5                   10                  15                

tta ggt gaa aag aac aag att ctt gtt gcc aac cgt ggt gaa att cca         96
Leu Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro           
            20                  25                  30                    

att aga atc ttt aga acg gct cat gaa ctt tcg atg aag act gtt gcg        144
Ile Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Lys Thr Val Ala           
        35                  40                  45                        

atc tat tcg cac gag gat aga cta tct atg cac aga ttg aag gca gac        192
Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys Ala Asp           
    50                  55                  60                            

gaa gct tac gtt att ggt gag cca gga aaa tac act cca gtt ggt gcg        240
Glu Ala Tyr Val Ile Gly Glu Pro Gly Lys Tyr Thr Pro Val Gly Ala           
65                  70                  75                  80            

tat ttg gcg atc gat gag att atc aag att gct caa ttg cac gga gtg        288
Tyr Leu Ala Ile Asp Glu Ile Ile Lys Ile Ala Gln Leu His Gly Val           
                85                  90                  95                

agc ttc atc cac cct ggt tat ggg ttc tta tcg gaa aac tct gag ttt        336
Ser Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe           
            100                 105                 110                   

gcc aag aag gtg gcc gac tct ggt atc acg tgg gtt ggt cct cca gcc        384
Ala Lys Lys Val Ala Asp Ser Gly Ile Thr Trp Val Gly Pro Pro Ala           
        115                 120                 125                       

gat gtg atc gat gct gtt ggt gac aag gtt tct gcc aga aac ttg gcc        432
Asp Val Ile Asp Ala Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala           
    130                 135                 140                           

gag aga gcg gat gtt cca gtg gtt cca ggt acg cct ggt cca ata gag        480
Glu Arg Ala Asp Val Pro Val Val Pro Gly Thr Pro Gly Pro Ile Glu           
145                 150                 155                 160           

aca gtt gaa gaa gca gtt gaa ttt gtg gag aag tac gga tac cca gtc        528
Thr Val Glu Glu Ala Val Glu Phe Val Glu Lys Tyr Gly Tyr Pro Val           
                165                 170                 175               

atc atc aag gct gcc ttc ggt ggt ggt ggt cgt ggt atg aga gtt gtt        576
Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val           
            180                 185                 190                   

cgt gaa ggt gat gat atc gcc gat gct ttc caa aga gcc aag tcc gaa        624
Arg Glu Gly Asp Asp Ile Ala Asp Ala Phe Gln Arg Ala Lys Ser Glu           
        195                 200                 205                       

gct gtt act gct ttc ggt aac ggt act tgt ttc gtt gaa aga ttc ttg        672
Ala Val Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu           
    210                 215                 220                           

gac aag cca aag cac atc gaa gtt cag ttg ttg gct gat cac tac ggt        720
Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp His Tyr Gly           
225                 230                 235                 240           

aat gtc atc cat cta ttc gaa aga gac tgt tct gtg caa aga aga cat        768
Asn Val Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His           
                245                 250                 255               

caa aag gtc gtt gaa gta gcg cca gcc aag act ttg cca gag agc gtg        816
Gln Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Glu Ser Val           
            260                 265                 270                   

cgt aat gca atc ttg act gac gct gtc aag ttg gct aag gag gca gga        864
Arg Asn Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Ala Gly           
        275                 280                 285                       

tac aga aat gct ggt acc gct gaa ttt ttg gtc gac aac caa aac aga        912
Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg           
    290                 295                 300                           

cac tac ttt att gaa atc aac cca aga att caa gtc gaa cat acc atc        960
His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile           
305                 310                 315                 320           

acc gaa gaa att acc ggt atc gac att gtc gcc gca caa att caa atc       1008
Thr Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile           
                325                 330                 335               

gca gca ggt gct tcc ttg gaa caa ttg gga cta ttg caa gat aga atc       1056
Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln Asp Arg Ile           
            340                 345                 350                   

acc acc cgt ggt ttc gct att caa tgt cgt atc act act gaa gat cct       1104
Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro           
        355                 360                 365                       

tcc aag aac ttc cag cca gat act ggt cgt atc gat gtt tac cgt tcc       1152
Ser Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Asp Val Tyr Arg Ser           
    370                 375                 380                           

gct ggt ggt aac ggt gtc aga ttg gat ggt ggt aac gca ttc gct ggt       1200
Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Phe Ala Gly           
385                 390                 395                 400           

tcg gtc att tca cct cat tat gat tcc atg ttg gtc aaa tgt tct tgt       1248
Ser Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys           
                405                 410                 415               

tcc ggt tcc act tac gaa atc gtt cgt cgt aag atg ttg cgt gcc ttg       1296
Ser Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Leu Arg Ala Leu           
            420                 425                 430                   

atc gaa ttc aga atc aga ggt gtg aag aca aac att cca ttc ttg cta       1344
Ile Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu           
        435                 440                 445                       

acg ttg ttg act cat cct gtg ttc aag tcc ggt gac tac tgg act acc       1392
Thr Leu Leu Thr His Pro Val Phe Lys Ser Gly Asp Tyr Trp Thr Thr           
    450                 455                 460                           

ttc atc gat gac act cca caa ttg ttc gaa atg gtt tct tct caa aac       1440
Phe Ile Asp Asp Thr Pro Gln Leu Phe Glu Met Val Ser Ser Gln Asn           
465                 470                 475                 480           

aga gca caa aaa cta ttg cac tac ttg gcc gat ctt gcc gtt aac ggt       1488
Arg Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Leu Ala Val Asn Gly           
                485                 490                 495               

tca tcg atc aag ggt caa att ggt cta cca aag tta aag act cat cct       1536
Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Thr His Pro           
            500                 505                 510                   

act atc cca cat ttg cat aag gcc gat ggc tcc att cta gat gtg tct       1584
Thr Ile Pro His Leu His Lys Ala Asp Gly Ser Ile Leu Asp Val Ser           
        515                 520                 525                       

gcc aag cct cct gcc ggg tgg aga gat gtt cta ttg caa cac ggc cca       1632
Ala Lys Pro Pro Ala Gly Trp Arg Asp Val Leu Leu Gln His Gly Pro           
    530                 535                 540                           

gaa gaa ttt gca aag caa gtt aga aag ttc aag ggt act ttg cta atg       1680
Glu Glu Phe Ala Lys Gln Val Arg Lys Phe Lys Gly Thr Leu Leu Met           
545                 550                 555                 560           

gac acc acc tgg aga gat gct cat caa tct cta ttg gcc act aga gtc       1728
Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val           
                565                 570                 575               

aga act tac gat ttg gct gcc atc gct cca act act gct cat gct ttg       1776
Arg Thr Tyr Asp Leu Ala Ala Ile Ala Pro Thr Thr Ala His Ala Leu           
            580                 585                 590                   

agc ggt gct ttc gct ttg gaa tgt tgg ggt ggt gcc act ttc gat gtc       1824
Ser Gly Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val           
        595                 600                 605                       

tcc atg aga ttc ttg cac gaa gat cca tgg gaa cgt ttg aga act ttg       1872
Ser Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Thr Leu           
    610                 615                 620                           

aga aag ttg gtt cct aac att cca ttc caa atg ttg cta cgt ggt gcc       1920
Arg Lys Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala           
625                 630                 635                 640           

aac ggt gtt gca tac tct tct cta cca gat aac gct atc gac cac ttt       1968
Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe           
                645                 650                 655               

gtc aag caa gca aag gat aac ggt gtt gac att ttc aga gtc ttc gat       2016
Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp           
            660                 665                 670                   

gct cta aac gat ttg gag caa ttg act gtc ggt gtt gac gct gtc aag       2064
Ala Leu Asn Asp Leu Glu Gln Leu Thr Val Gly Val Asp Ala Val Lys           
        675                 680                 685                       

aag gct ggt ggt gtt gtc gaa gct acc att tgt tac tcc ggt gac atg       2112
Lys Ala Gly Gly Val Val Glu Ala Thr Ile Cys Tyr Ser Gly Asp Met           
    690                 695                 700                           

cta gca cca ggt aag aag tac aac ctt gac tac tac ttg gac att gtt       2160
Leu Ala Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Asp Ile Val           
705                 710                 715                 720           

gaa caa gtg gtt aag aga ggt acc cat att ctt ggt atc aag gat atg       2208
Glu Gln Val Val Lys Arg Gly Thr His Ile Leu Gly Ile Lys Asp Met           
                725                 730                 735               

gca ggt act ttg aag cca tct gct gct aag ctc ttg atc ggt tct atc       2256
Ala Gly Thr Leu Lys Pro Ser Ala Ala Lys Leu Leu Ile Gly Ser Ile           
            740                 745                 750                   

aga aca aag tac cct gac ttg cca att cac gtc cat acc cat gac tcc       2304
Arg Thr Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser           
        755                 760                 765                       

gcc ggt acc ggt gtt gct tcc atg gct gca tgt gct ttc gct ggt gct       2352
Ala Gly Thr Gly Val Ala Ser Met Ala Ala Cys Ala Phe Ala Gly Ala           
    770                 775                 780                           

gat gtt gtt gat gtt gca acc aac tct atg tct ggt atg act tct caa       2400
Asp Val Val Asp Val Ala Thr Asn Ser Met Ser Gly Met Thr Ser Gln           
785                 790                 795                 800           

cca tct gtc aat gca cta ttg gct gct ctt gat ggt gaa atc gac tgt       2448
Pro Ser Val Asn Ala Leu Leu Ala Ala Leu Asp Gly Glu Ile Asp Cys           
                805                 810                 815               

aat gtc aac gtc agc tac atc agt cag cta gat gct tac tgg gct gaa       2496
Asn Val Asn Val Ser Tyr Ile Ser Gln Leu Asp Ala Tyr Trp Ala Glu           
            820                 825                 830                   

atg aga cta ttg tac tca tgt ttc gaa gcc gac ttg aag ggt cct gat       2544
Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp           
        835                 840                 845                       

cca gaa gtt tac gtc cat gaa att cca ggt ggt caa ttg acc aac ttg       2592
Pro Glu Val Tyr Val His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu           
    850                 855                 860                           

ctc ttc caa gcc caa caa ttg ggt ctt ggt gag caa tgg gct gaa acc       2640
Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr           
865                 870                 875                 880           

aag aga gct tac cgt gaa gca aac ctg ttg ttg ggt gat gtt gtt aag       2688
Lys Arg Ala Tyr Arg Glu Ala Asn Leu Leu Leu Gly Asp Val Val Lys           
                885                 890                 895               

gtc act cca aca tcc aag gtt gtc ggt gat ttg gct caa ttc atg gtc       2736
Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val           
            900                 905                 910                   

act aac aag ttg acc tcg gat gat gtt aag aga tta gct tca tct ttg       2784
Thr Asn Lys Leu Thr Ser Asp Asp Val Lys Arg Leu Ala Ser Ser Leu           
        915                 920                 925                       

gat ttc cca gac tcc gtc atg gac ttc ttt gaa ggt tta atc ggt caa       2832
Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln           
    930                 935                 940                           

cca tac ggt ggt ttc cca gaa cct cta aga tct gat gtt ttg aag aac       2880
Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Ser Asp Val Leu Lys Asn           
945                 950                 955                 960           

aag aga aga aag ttg acc aag aga cca ggt ttg gaa ttg gct cca ttc       2928
Lys Arg Arg Lys Leu Thr Lys Arg Pro Gly Leu Glu Leu Ala Pro Phe           
                965                 970                 975               

gat ttg gaa ggc att aag gaa gat ttg act aac aga ttt ggt gac att       2976
Asp Leu Glu Gly Ile Lys Glu Asp Leu Thr Asn Arg Phe Gly Asp Ile           
            980                 985                 990                   

gac gac tgt gat gtt gct tct tac  aac atg tat cca aag  gtc tac gaa     3024
Asp Asp Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Lys  Val Tyr Glu         
        995                 1000                 1005                     

gat ttc  cgt aag atc aga gaa  aag tac ggt gat cta  tct gtt ttg        3069
Asp Phe  Arg Lys Ile Arg Glu  Lys Tyr Gly Asp Leu  Ser Val Leu            
    1010                 1015                 1020                        

cca acc  aag aac ttc ttg tct  cca cct tca atc ggt  gaa gaa atc        3114
Pro Thr  Lys Asn Phe Leu Ser  Pro Pro Ser Ile Gly  Glu Glu Ile            
    1025                 1030                 1035                        

gtc gtt  aca att gaa caa ggt  aag act ttg atc att  aag cca caa        3159
Val Val  Thr Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Pro Gln            
    1040                 1045                 1050                        

gct att  ggt gat ttg aac aag  gag act ggt atc aga  gaa gtt tac        3204
Ala Ile  Gly Asp Leu Asn Lys  Glu Thr Gly Ile Arg  Glu Val Tyr            
    1055                 1060                 1065                        

ttc gaa  ttg aac ggt gaa ttg  aga aag gtc tct gtt  gct gac aga        3249
Phe Glu  Leu Asn Gly Glu Leu  Arg Lys Val Ser Val  Ala Asp Arg            
    1070                 1075                 1080                        

tct caa  aag gtt gaa acg atc  tcc aag cca aag gct  gac gcc cac        3294
Ser Gln  Lys Val Glu Thr Ile  Ser Lys Pro Lys Ala  Asp Ala His            
    1085                 1090                 1095                        

gat cca  ttc caa gtt ggt tct  cca atg gca ggt gtt  gtt gtc gaa        3339
Asp Pro  Phe Gln Val Gly Ser  Pro Met Ala Gly Val  Val Val Glu            
    1100                 1105                 1110                        

gtc aag  gta cac aag ggt tct  ttg atc tcc aag ggc  caa cca gtc        3384
Val Lys  Val His Lys Gly Ser  Leu Ile Ser Lys Gly  Gln Pro Val            
    1115                 1120                 1125                        

gct gtc  cta agt gcc atg aag  atg gaa atg gtt atc  tcc tcc cca        3429
Ala Val  Leu Ser Ala Met Lys  Met Glu Met Val Ile  Ser Ser Pro            
    1130                 1135                 1140                        

tct gat  ggt caa gtc aag gaa  gtg ctt gtc aag gat  ggt gaa aac        3474
Ser Asp  Gly Gln Val Lys Glu  Val Leu Val Lys Asp  Gly Glu Asn            
    1145                 1150                 1155                        

gtt gac  gct tct gac ttg ctc  gtt gtt ttg gaa gaa  gct cca gct        3519
Val Asp  Ala Ser Asp Leu Leu  Val Val Leu Glu Glu  Ala Pro Ala            
    1160                 1165                 1170                        

aaa gaa  taa                                                          3528
Lys Glu                                                                   
    1175                                                                  


<210>  12
<211>  1175
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  12

Met Ser Thr Gln Asn Asp Leu Ala Gly Leu Arg Asp Asn Ser Asn Leu 
1               5                   10                  15      


Leu Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro 
            20                  25                  30          


Ile Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Lys Thr Val Ala 
        35                  40                  45              


Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys Ala Asp 
    50                  55                  60                  


Glu Ala Tyr Val Ile Gly Glu Pro Gly Lys Tyr Thr Pro Val Gly Ala 
65                  70                  75                  80  


Tyr Leu Ala Ile Asp Glu Ile Ile Lys Ile Ala Gln Leu His Gly Val 
                85                  90                  95      


Ser Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe 
            100                 105                 110         


Ala Lys Lys Val Ala Asp Ser Gly Ile Thr Trp Val Gly Pro Pro Ala 
        115                 120                 125             


Asp Val Ile Asp Ala Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala 
    130                 135                 140                 


Glu Arg Ala Asp Val Pro Val Val Pro Gly Thr Pro Gly Pro Ile Glu 
145                 150                 155                 160 


Thr Val Glu Glu Ala Val Glu Phe Val Glu Lys Tyr Gly Tyr Pro Val 
                165                 170                 175     


Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val 
            180                 185                 190         


Arg Glu Gly Asp Asp Ile Ala Asp Ala Phe Gln Arg Ala Lys Ser Glu 
        195                 200                 205             


Ala Val Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu 
    210                 215                 220                 


Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp His Tyr Gly 
225                 230                 235                 240 


Asn Val Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His 
                245                 250                 255     


Gln Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Glu Ser Val 
            260                 265                 270         


Arg Asn Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Ala Gly 
        275                 280                 285             


Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg 
    290                 295                 300                 


His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile 
305                 310                 315                 320 


Thr Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile 
                325                 330                 335     


Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln Asp Arg Ile 
            340                 345                 350         


Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro 
        355                 360                 365             


Ser Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Asp Val Tyr Arg Ser 
    370                 375                 380                 


Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Phe Ala Gly 
385                 390                 395                 400 


Ser Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys 
                405                 410                 415     


Ser Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Leu Arg Ala Leu 
            420                 425                 430         


Ile Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu 
        435                 440                 445             


Thr Leu Leu Thr His Pro Val Phe Lys Ser Gly Asp Tyr Trp Thr Thr 
    450                 455                 460                 


Phe Ile Asp Asp Thr Pro Gln Leu Phe Glu Met Val Ser Ser Gln Asn 
465                 470                 475                 480 


Arg Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Leu Ala Val Asn Gly 
                485                 490                 495     


Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Thr His Pro 
            500                 505                 510         


Thr Ile Pro His Leu His Lys Ala Asp Gly Ser Ile Leu Asp Val Ser 
        515                 520                 525             


Ala Lys Pro Pro Ala Gly Trp Arg Asp Val Leu Leu Gln His Gly Pro 
    530                 535                 540                 


Glu Glu Phe Ala Lys Gln Val Arg Lys Phe Lys Gly Thr Leu Leu Met 
545                 550                 555                 560 


Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val 
                565                 570                 575     


Arg Thr Tyr Asp Leu Ala Ala Ile Ala Pro Thr Thr Ala His Ala Leu 
            580                 585                 590         


Ser Gly Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val 
        595                 600                 605             


Ser Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Thr Leu 
    610                 615                 620                 


Arg Lys Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala 
625                 630                 635                 640 


Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe 
                645                 650                 655     


Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp 
            660                 665                 670         


Ala Leu Asn Asp Leu Glu Gln Leu Thr Val Gly Val Asp Ala Val Lys 
        675                 680                 685             


Lys Ala Gly Gly Val Val Glu Ala Thr Ile Cys Tyr Ser Gly Asp Met 
    690                 695                 700                 


Leu Ala Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Asp Ile Val 
705                 710                 715                 720 


Glu Gln Val Val Lys Arg Gly Thr His Ile Leu Gly Ile Lys Asp Met 
                725                 730                 735     


Ala Gly Thr Leu Lys Pro Ser Ala Ala Lys Leu Leu Ile Gly Ser Ile 
            740                 745                 750         


Arg Thr Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser 
        755                 760                 765             


Ala Gly Thr Gly Val Ala Ser Met Ala Ala Cys Ala Phe Ala Gly Ala 
    770                 775                 780                 


Asp Val Val Asp Val Ala Thr Asn Ser Met Ser Gly Met Thr Ser Gln 
785                 790                 795                 800 


Pro Ser Val Asn Ala Leu Leu Ala Ala Leu Asp Gly Glu Ile Asp Cys 
                805                 810                 815     


Asn Val Asn Val Ser Tyr Ile Ser Gln Leu Asp Ala Tyr Trp Ala Glu 
            820                 825                 830         


Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp 
        835                 840                 845             


Pro Glu Val Tyr Val His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu 
    850                 855                 860                 


Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr 
865                 870                 875                 880 


Lys Arg Ala Tyr Arg Glu Ala Asn Leu Leu Leu Gly Asp Val Val Lys 
                885                 890                 895     


Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val 
            900                 905                 910         


Thr Asn Lys Leu Thr Ser Asp Asp Val Lys Arg Leu Ala Ser Ser Leu 
        915                 920                 925             


Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln 
    930                 935                 940                 


Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Ser Asp Val Leu Lys Asn 
945                 950                 955                 960 


Lys Arg Arg Lys Leu Thr Lys Arg Pro Gly Leu Glu Leu Ala Pro Phe 
                965                 970                 975     


Asp Leu Glu Gly Ile Lys Glu Asp Leu Thr Asn Arg Phe Gly Asp Ile 
            980                 985                 990         


Asp Asp Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Lys  Val Tyr Glu 
        995                 1000                 1005             


Asp Phe  Arg Lys Ile Arg Glu  Lys Tyr Gly Asp Leu  Ser Val Leu 
    1010                 1015                 1020             


Pro Thr  Lys Asn Phe Leu Ser  Pro Pro Ser Ile Gly  Glu Glu Ile 
    1025                 1030                 1035             


Val Val  Thr Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Pro Gln 
    1040                 1045                 1050             


Ala Ile  Gly Asp Leu Asn Lys  Glu Thr Gly Ile Arg  Glu Val Tyr 
    1055                 1060                 1065             


Phe Glu  Leu Asn Gly Glu Leu  Arg Lys Val Ser Val  Ala Asp Arg 
    1070                 1075                 1080             


Ser Gln  Lys Val Glu Thr Ile  Ser Lys Pro Lys Ala  Asp Ala His 
    1085                 1090                 1095             


Asp Pro  Phe Gln Val Gly Ser  Pro Met Ala Gly Val  Val Val Glu 
    1100                 1105                 1110             


Val Lys  Val His Lys Gly Ser  Leu Ile Ser Lys Gly  Gln Pro Val 
    1115                 1120                 1125             


Ala Val  Leu Ser Ala Met Lys  Met Glu Met Val Ile  Ser Ser Pro 
    1130                 1135                 1140             


Ser Asp  Gly Gln Val Lys Glu  Val Leu Val Lys Asp  Gly Glu Asn 
    1145                 1150                 1155             


Val Asp  Ala Ser Asp Leu Leu  Val Val Leu Glu Glu  Ala Pro Ala 
    1160                 1165                 1170             


Lys Glu  
    1175 


<210>  13
<211>  1029
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1029)

<400>  13
atg tcc aat gtt aaa gta gct cta cta ggt gcc gct ggt ggt atc ggc         48
Met Ser Asn Val Lys Val Ala Leu Leu Gly Ala Ala Gly Gly Ile Gly           
1               5                   10                  15                

caa cca ctt gct cta tta ctt aag ctt aat cca aac ata acc cat ttg         96
Gln Pro Leu Ala Leu Leu Leu Lys Leu Asn Pro Asn Ile Thr His Leu           
            20                  25                  30                    

gca ctc tat gac gtt gtg cat gtt cct gga gtg gct gcc gac cta cac        144
Ala Leu Tyr Asp Val Val His Val Pro Gly Val Ala Ala Asp Leu His           
        35                  40                  45                        

cat ata gac aca gat gta gtg att acc cac cat ttg aaa gat gaa gac        192
His Ile Asp Thr Asp Val Val Ile Thr His His Leu Lys Asp Glu Asp           
    50                  55                  60                            

ggt acg gcc ttg gca aac gcc ctc aag gac gct acg ttt gtt att gtc        240
Gly Thr Ala Leu Ala Asn Ala Leu Lys Asp Ala Thr Phe Val Ile Val           
65                  70                  75                  80            

ccc gcc ggt gtt ccg aga aag ccc ggc atg act aga ggt gat ttg ttc        288
Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Gly Asp Leu Phe           
                85                  90                  95                

aca att aat gcc ggt ata tgt gcc gaa ttg gct aat gct att agt ttg        336
Thr Ile Asn Ala Gly Ile Cys Ala Glu Leu Ala Asn Ala Ile Ser Leu           
            100                 105                 110                   

aac gct cct aat gca ttc acc ctt gtc att acc aat ccg gtc aac tcg        384
Asn Ala Pro Asn Ala Phe Thr Leu Val Ile Thr Asn Pro Val Asn Ser           
        115                 120                 125                       

acc gtt cct ata ttt aag gaa ata ttt gct aaa aat gaa gcc ttc aat        432
Thr Val Pro Ile Phe Lys Glu Ile Phe Ala Lys Asn Glu Ala Phe Asn           
    130                 135                 140                           

cca agg aga ctg ttt ggt gta act gct cta gat cat gtt aga tca aat        480
Pro Arg Arg Leu Phe Gly Val Thr Ala Leu Asp His Val Arg Ser Asn           
145                 150                 155                 160           

act ttt ctc tcg gaa tta att gac ggt aaa aat ccc caa cat ttt gat        528
Thr Phe Leu Ser Glu Leu Ile Asp Gly Lys Asn Pro Gln His Phe Asp           
                165                 170                 175               

gtc act gtt gtt ggc gga cac tct ggt aac tca att gtc ccc cta ttc        576
Val Thr Val Val Gly Gly His Ser Gly Asn Ser Ile Val Pro Leu Phe           
            180                 185                 190                   

tcc ctt gtt aag gct gcc gaa aat tta gac gat gaa att ata gat gcc        624
Ser Leu Val Lys Ala Ala Glu Asn Leu Asp Asp Glu Ile Ile Asp Ala           
        195                 200                 205                       

ttg att cat aga gtt caa tac ggt gga gat gaa gtt gtg gaa gca aag        672
Leu Ile His Arg Val Gln Tyr Gly Gly Asp Glu Val Val Glu Ala Lys           
    210                 215                 220                           

agc ggt gcg ggc tcg gca act ctt tca atg gct tat gcc gct aac aag        720
Ser Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Ala Asn Lys           
225                 230                 235                 240           

ttc ttc aat ata ttg ctt aat gga tac ttg ggt ttg aag aag aca atg        768
Phe Phe Asn Ile Leu Leu Asn Gly Tyr Leu Gly Leu Lys Lys Thr Met           
                245                 250                 255               

att tca agt tat gtc ttt tta gac gat tca atc aac ggc gtc cct caa        816
Ile Ser Ser Tyr Val Phe Leu Asp Asp Ser Ile Asn Gly Val Pro Gln           
            260                 265                 270                   

tta aag gaa aat ttg tct aaa ctt ttg aaa ggt tcc gag gtt gag tta        864
Leu Lys Glu Asn Leu Ser Lys Leu Leu Lys Gly Ser Glu Val Glu Leu           
        275                 280                 285                       

cca agt tat ttg gct gtt cca atg acc tat ggt aaa gaa ggt att gaa        912
Pro Ser Tyr Leu Ala Val Pro Met Thr Tyr Gly Lys Glu Gly Ile Glu           
    290                 295                 300                           

caa gtc ttt tac gat tgg gtg ttt gaa atg tca cca aag gaa aag gaa        960
Gln Val Phe Tyr Asp Trp Val Phe Glu Met Ser Pro Lys Glu Lys Glu           
305                 310                 315                 320           

aac ttc att aca gcg att gaa tac att gat caa aat att gaa aaa ggt       1008
Asn Phe Ile Thr Ala Ile Glu Tyr Ile Asp Gln Asn Ile Glu Lys Gly           
                325                 330                 335               

ctg aat ttt atg gta cgt taa                                           1029
Leu Asn Phe Met Val Arg                                                   
            340                                                           


<210>  14
<211>  342
<212>  PRT
<213>  Issatchenkia orientalis

<400>  14

Met Ser Asn Val Lys Val Ala Leu Leu Gly Ala Ala Gly Gly Ile Gly 
1               5                   10                  15      


Gln Pro Leu Ala Leu Leu Leu Lys Leu Asn Pro Asn Ile Thr His Leu 
            20                  25                  30          


Ala Leu Tyr Asp Val Val His Val Pro Gly Val Ala Ala Asp Leu His 
        35                  40                  45              


His Ile Asp Thr Asp Val Val Ile Thr His His Leu Lys Asp Glu Asp 
    50                  55                  60                  


Gly Thr Ala Leu Ala Asn Ala Leu Lys Asp Ala Thr Phe Val Ile Val 
65                  70                  75                  80  


Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Gly Asp Leu Phe 
                85                  90                  95      


Thr Ile Asn Ala Gly Ile Cys Ala Glu Leu Ala Asn Ala Ile Ser Leu 
            100                 105                 110         


Asn Ala Pro Asn Ala Phe Thr Leu Val Ile Thr Asn Pro Val Asn Ser 
        115                 120                 125             


Thr Val Pro Ile Phe Lys Glu Ile Phe Ala Lys Asn Glu Ala Phe Asn 
    130                 135                 140                 


Pro Arg Arg Leu Phe Gly Val Thr Ala Leu Asp His Val Arg Ser Asn 
145                 150                 155                 160 


Thr Phe Leu Ser Glu Leu Ile Asp Gly Lys Asn Pro Gln His Phe Asp 
                165                 170                 175     


Val Thr Val Val Gly Gly His Ser Gly Asn Ser Ile Val Pro Leu Phe 
            180                 185                 190         


Ser Leu Val Lys Ala Ala Glu Asn Leu Asp Asp Glu Ile Ile Asp Ala 
        195                 200                 205             


Leu Ile His Arg Val Gln Tyr Gly Gly Asp Glu Val Val Glu Ala Lys 
    210                 215                 220                 


Ser Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Ala Asn Lys 
225                 230                 235                 240 


Phe Phe Asn Ile Leu Leu Asn Gly Tyr Leu Gly Leu Lys Lys Thr Met 
                245                 250                 255     


Ile Ser Ser Tyr Val Phe Leu Asp Asp Ser Ile Asn Gly Val Pro Gln 
            260                 265                 270         


Leu Lys Glu Asn Leu Ser Lys Leu Leu Lys Gly Ser Glu Val Glu Leu 
        275                 280                 285             


Pro Ser Tyr Leu Ala Val Pro Met Thr Tyr Gly Lys Glu Gly Ile Glu 
    290                 295                 300                 


Gln Val Phe Tyr Asp Trp Val Phe Glu Met Ser Pro Lys Glu Lys Glu 
305                 310                 315                 320 


Asn Phe Ile Thr Ala Ile Glu Tyr Ile Asp Gln Asn Ile Glu Lys Gly 
                325                 330                 335     


Leu Asn Phe Met Val Arg 
            340         


<210>  15
<211>  1011
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1011)

<400>  15
atg gtc aag gtg act att tta ggc gct gcc ggt gga att gga caa cca         48
Met Val Lys Val Thr Ile Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro           
1               5                   10                  15                

ctc tca ttg tta ttg aga ctt aat cca tgg att gac gaa ttg gcc ttg         96
Leu Ser Leu Leu Leu Arg Leu Asn Pro Trp Ile Asp Glu Leu Ala Leu           
            20                  25                  30                    

ttt gat att gtc aat acc ccc ggc gtg agt tgt gat ttg tcg cat att        144
Phe Asp Ile Val Asn Thr Pro Gly Val Ser Cys Asp Leu Ser His Ile           
        35                  40                  45                        

cct gca tca cag gtt gtt aat ggc tat gct ccg aaa tcg aaa tca gat        192
Pro Ala Ser Gln Val Val Asn Gly Tyr Ala Pro Lys Ser Lys Ser Asp           
    50                  55                  60                            

aca gag aca atc aag act gcc ttg aaa ggt gct gat att gtt gtt att        240
Thr Glu Thr Ile Lys Thr Ala Leu Lys Gly Ala Asp Ile Val Val Ile           
65                  70                  75                  80            

cct gca gga att cca cgt aaa cct ggt atg aca aga aac gat ctc ttt        288
Pro Ala Gly Ile Pro Arg Lys Pro Gly Met Thr Arg Asn Asp Leu Phe           
                85                  90                  95                

aaa atc aat gcc gga atc gtt aag agt ttg att cat agt gca gga acc        336
Lys Ile Asn Ala Gly Ile Val Lys Ser Leu Ile His Ser Ala Gly Thr           
            100                 105                 110                   

act tgc cct gat gca ttt att tgt gtc att tcg aac cct gtc aac tcg        384
Thr Cys Pro Asp Ala Phe Ile Cys Val Ile Ser Asn Pro Val Asn Ser           
        115                 120                 125                       

aca gtt cca att gcc gtt gaa gaa cta aag cgt ttg aat gtt ttt aat        432
Thr Val Pro Ile Ala Val Glu Glu Leu Lys Arg Leu Asn Val Phe Asn           
    130                 135                 140                           

cca cat aaa gtt ttc ggt att acc aca ttg gac aat ttc aga tta gaa        480
Pro His Lys Val Phe Gly Ile Thr Thr Leu Asp Asn Phe Arg Leu Glu           
145                 150                 155                 160           

gaa ttt ctg agt gga gaa ctt ggt gga att gtc aaa cca aat gat tta        528
Glu Phe Leu Ser Gly Glu Leu Gly Gly Ile Val Lys Pro Asn Asp Leu           
                165                 170                 175               

tat ggt gat gta gtt gct ata ggt ggc cat tcg ggc gac tct ata gta        576
Tyr Gly Asp Val Val Ala Ile Gly Gly His Ser Gly Asp Ser Ile Val           
            180                 185                 190                   

ccg atc ttg aat tcg tgg aat ttg aat ttc atc aat gat gga gat tct        624
Pro Ile Leu Asn Ser Trp Asn Leu Asn Phe Ile Asn Asp Gly Asp Ser           
        195                 200                 205                       

tat aac aat ttg gtc aag agg gtc cag ttt gga ggc gat gag gtt gtc        672
Tyr Asn Asn Leu Val Lys Arg Val Gln Phe Gly Gly Asp Glu Val Val           
    210                 215                 220                           

aag gca aag gac ggg aaa ggt tcg gct aca ttg tca atg gct aca gct        720
Lys Ala Lys Asp Gly Lys Gly Ser Ala Thr Leu Ser Met Ala Thr Ala           
225                 230                 235                 240           

gca tac agg ttt gtc aac aac ctc ttg gac gcc att gtc aat aac aag        768
Ala Tyr Arg Phe Val Asn Asn Leu Leu Asp Ala Ile Val Asn Asn Lys           
                245                 250                 255               

aaa gtc aag gaa gtg gcc ttt gtg aaa atc gac caa ttg cca act aca        816
Lys Val Lys Glu Val Ala Phe Val Lys Ile Asp Gln Leu Pro Thr Thr           
            260                 265                 270                   

agg gtt cct tat ttt gtt gtt gat gaa act cag tat ttt agt cta ccc        864
Arg Val Pro Tyr Phe Val Val Asp Glu Thr Gln Tyr Phe Ser Leu Pro           
        275                 280                 285                       

att att ctc ggt aga cag ggg att gag agg gtc acg ttc cca gaa tct        912
Ile Ile Leu Gly Arg Gln Gly Ile Glu Arg Val Thr Phe Pro Glu Ser           
    290                 295                 300                           

ctg aca gag caa gag gtg aga atg aca aag cac gct gtt gct aaa gtt        960
Leu Thr Glu Gln Glu Val Arg Met Thr Lys His Ala Val Ala Lys Val           
305                 310                 315                 320           

aaa gtt gac gtt aat aaa ggc ttc aat ttt gtc cat ggc cca aaa ctg       1008
Lys Val Asp Val Asn Lys Gly Phe Asn Phe Val His Gly Pro Lys Leu           
                325                 330                 335               

taa                                                                   1011


<210>  16
<211>  336
<212>  PRT
<213>  Issatchenkia orientalis

<400>  16

Met Val Lys Val Thr Ile Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Arg Leu Asn Pro Trp Ile Asp Glu Leu Ala Leu 
            20                  25                  30          


Phe Asp Ile Val Asn Thr Pro Gly Val Ser Cys Asp Leu Ser His Ile 
        35                  40                  45              


Pro Ala Ser Gln Val Val Asn Gly Tyr Ala Pro Lys Ser Lys Ser Asp 
    50                  55                  60                  


Thr Glu Thr Ile Lys Thr Ala Leu Lys Gly Ala Asp Ile Val Val Ile 
65                  70                  75                  80  


Pro Ala Gly Ile Pro Arg Lys Pro Gly Met Thr Arg Asn Asp Leu Phe 
                85                  90                  95      


Lys Ile Asn Ala Gly Ile Val Lys Ser Leu Ile His Ser Ala Gly Thr 
            100                 105                 110         


Thr Cys Pro Asp Ala Phe Ile Cys Val Ile Ser Asn Pro Val Asn Ser 
        115                 120                 125             


Thr Val Pro Ile Ala Val Glu Glu Leu Lys Arg Leu Asn Val Phe Asn 
    130                 135                 140                 


Pro His Lys Val Phe Gly Ile Thr Thr Leu Asp Asn Phe Arg Leu Glu 
145                 150                 155                 160 


Glu Phe Leu Ser Gly Glu Leu Gly Gly Ile Val Lys Pro Asn Asp Leu 
                165                 170                 175     


Tyr Gly Asp Val Val Ala Ile Gly Gly His Ser Gly Asp Ser Ile Val 
            180                 185                 190         


Pro Ile Leu Asn Ser Trp Asn Leu Asn Phe Ile Asn Asp Gly Asp Ser 
        195                 200                 205             


Tyr Asn Asn Leu Val Lys Arg Val Gln Phe Gly Gly Asp Glu Val Val 
    210                 215                 220                 


Lys Ala Lys Asp Gly Lys Gly Ser Ala Thr Leu Ser Met Ala Thr Ala 
225                 230                 235                 240 


Ala Tyr Arg Phe Val Asn Asn Leu Leu Asp Ala Ile Val Asn Asn Lys 
                245                 250                 255     


Lys Val Lys Glu Val Ala Phe Val Lys Ile Asp Gln Leu Pro Thr Thr 
            260                 265                 270         


Arg Val Pro Tyr Phe Val Val Asp Glu Thr Gln Tyr Phe Ser Leu Pro 
        275                 280                 285             


Ile Ile Leu Gly Arg Gln Gly Ile Glu Arg Val Thr Phe Pro Glu Ser 
    290                 295                 300                 


Leu Thr Glu Gln Glu Val Arg Met Thr Lys His Ala Val Ala Lys Val 
305                 310                 315                 320 


Lys Val Asp Val Asn Lys Gly Phe Asn Phe Val His Gly Pro Lys Leu 
                325                 330                 335     


<210>  17
<211>  1005
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1005)

<400>  17
atg ttc tcc aga atc tct gct aga caa ttc tcc tcc tct gct gct tcc         48
Met Phe Ser Arg Ile Ser Ala Arg Gln Phe Ser Ser Ser Ala Ala Ser           
1               5                   10                  15                

gct tac aag gtc acc gtt tta ggt gct gca ggt ggt att ggc caa cca         96
Ala Tyr Lys Val Thr Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro           
            20                  25                  30                    

cta tct ctt ttg atg aag ttg aac cac aag gtc acc aac tta tcc ttg        144
Leu Ser Leu Leu Met Lys Leu Asn His Lys Val Thr Asn Leu Ser Leu           
        35                  40                  45                        

tac gac ttg aga ttg ggt gct ggt gtt gcc act gac ttg tcc cac att        192
Tyr Asp Leu Arg Leu Gly Ala Gly Val Ala Thr Asp Leu Ser His Ile           
    50                  55                  60                            

cca acc aac tcc gtt gtc aag ggc tat ggt cca gaa aac aat ggt ttg        240
Pro Thr Asn Ser Val Val Lys Gly Tyr Gly Pro Glu Asn Asn Gly Leu           
65                  70                  75                  80            

aag gac gcc ttg acc ggc tcc gat gtt gtt ctt att cca gct ggt gtt        288
Lys Asp Ala Leu Thr Gly Ser Asp Val Val Leu Ile Pro Ala Gly Val           
                85                  90                  95                

cca aga aaa cca ggt atg act aga gac gat ctc ttc aac acc aat gca        336
Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Asn Thr Asn Ala           
            100                 105                 110                   

tcg att gtc aga gac ttg gca aag gct gct gca gac cac tgt cca aac        384
Ser Ile Val Arg Asp Leu Ala Lys Ala Ala Ala Asp His Cys Pro Asn           
        115                 120                 125                       

gcc gtc ttg ttg atc att tca aac cct gtc aac tca act gtc cca att        432
Ala Val Leu Leu Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile           
    130                 135                 140                           

gtt gct gag gtt ttg aaa tca aag ggc gtc tac aac cca aag aag ttg        480
Val Ala Glu Val Leu Lys Ser Lys Gly Val Tyr Asn Pro Lys Lys Leu           
145                 150                 155                 160           

ttt ggt gtc acc act ttg gac gtt ttg aga tcc tcg aga ttc ttg agt        528
Phe Gly Val Thr Thr Leu Asp Val Leu Arg Ser Ser Arg Phe Leu Ser           
                165                 170                 175               

gaa gtc gtc aac acc gac cca acc acc gaa acc gtc act gtt gtt ggt        576
Glu Val Val Asn Thr Asp Pro Thr Thr Glu Thr Val Thr Val Val Gly           
            180                 185                 190                   

ggc cac tct ggt gtc acc att gtt cct tta atc tcc caa acc aaa cac        624
Gly His Ser Gly Val Thr Ile Val Pro Leu Ile Ser Gln Thr Lys His           
        195                 200                 205                       

aag gac ttg cca aag gaa acc tac gaa gca ttg gtc cac aga atc caa        672
Lys Asp Leu Pro Lys Glu Thr Tyr Glu Ala Leu Val His Arg Ile Gln           
    210                 215                 220                           

ttc ggt ggt gat gag gtt gtc aag gcc aag gac ggt gca ggt tcc gct        720
Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala           
225                 230                 235                 240           

acc ttg tcc atg gcc caa gcc ggt gca aga atg gcc tcc tcc gtc ttg        768
Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Met Ala Ser Ser Val Leu           
                245                 250                 255               

aag ggt ttg gct ggt gaa gtt gac att gtc gaa cca acc ttt att gac        816
Lys Gly Leu Ala Gly Glu Val Asp Ile Val Glu Pro Thr Phe Ile Asp           
            260                 265                 270                   

tct cca ttg ttc aag tcc gaa ggt gtc gaa ttc ttc tcc tcc aga gtc        864
Ser Pro Leu Phe Lys Ser Glu Gly Val Glu Phe Phe Ser Ser Arg Val           
        275                 280                 285                       

acc ctt ggt cca gaa ggt gtc caa gaa gtc cac cca ttg ggc gtc tta        912
Thr Leu Gly Pro Glu Gly Val Gln Glu Val His Pro Leu Gly Val Leu           
    290                 295                 300                           

tct act gct gaa gaa gaa atg gtt gct act gct aag gaa acc ttg aag        960
Ser Thr Ala Glu Glu Glu Met Val Ala Thr Ala Lys Glu Thr Leu Lys           
305                 310                 315                 320           

aag aac atc caa aag ggt gtc gac ttt gtc aag gct aac cca taa           1005
Lys Asn Ile Gln Lys Gly Val Asp Phe Val Lys Ala Asn Pro                   
                325                 330                                   


<210>  18
<211>  334
<212>  PRT
<213>  Issatchenkia orientalis

<400>  18

Met Phe Ser Arg Ile Ser Ala Arg Gln Phe Ser Ser Ser Ala Ala Ser 
1               5                   10                  15      


Ala Tyr Lys Val Thr Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro 
            20                  25                  30          


Leu Ser Leu Leu Met Lys Leu Asn His Lys Val Thr Asn Leu Ser Leu 
        35                  40                  45              


Tyr Asp Leu Arg Leu Gly Ala Gly Val Ala Thr Asp Leu Ser His Ile 
    50                  55                  60                  


Pro Thr Asn Ser Val Val Lys Gly Tyr Gly Pro Glu Asn Asn Gly Leu 
65                  70                  75                  80  


Lys Asp Ala Leu Thr Gly Ser Asp Val Val Leu Ile Pro Ala Gly Val 
                85                  90                  95      


Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Asn Thr Asn Ala 
            100                 105                 110         


Ser Ile Val Arg Asp Leu Ala Lys Ala Ala Ala Asp His Cys Pro Asn 
        115                 120                 125             


Ala Val Leu Leu Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile 
    130                 135                 140                 


Val Ala Glu Val Leu Lys Ser Lys Gly Val Tyr Asn Pro Lys Lys Leu 
145                 150                 155                 160 


Phe Gly Val Thr Thr Leu Asp Val Leu Arg Ser Ser Arg Phe Leu Ser 
                165                 170                 175     


Glu Val Val Asn Thr Asp Pro Thr Thr Glu Thr Val Thr Val Val Gly 
            180                 185                 190         


Gly His Ser Gly Val Thr Ile Val Pro Leu Ile Ser Gln Thr Lys His 
        195                 200                 205             


Lys Asp Leu Pro Lys Glu Thr Tyr Glu Ala Leu Val His Arg Ile Gln 
    210                 215                 220                 


Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala 
225                 230                 235                 240 


Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Met Ala Ser Ser Val Leu 
                245                 250                 255     


Lys Gly Leu Ala Gly Glu Val Asp Ile Val Glu Pro Thr Phe Ile Asp 
            260                 265                 270         


Ser Pro Leu Phe Lys Ser Glu Gly Val Glu Phe Phe Ser Ser Arg Val 
        275                 280                 285             


Thr Leu Gly Pro Glu Gly Val Gln Glu Val His Pro Leu Gly Val Leu 
    290                 295                 300                 


Ser Thr Ala Glu Glu Glu Met Val Ala Thr Ala Lys Glu Thr Leu Lys 
305                 310                 315                 320 


Lys Asn Ile Gln Lys Gly Val Asp Phe Val Lys Ala Asn Pro 
                325                 330                 


<210>  19
<211>  1017
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1017)

<400>  19
atg ctt aga gcc cta act cgc cgt caa ttt tcc tcc act gcc ttc aac         48
Met Leu Arg Ala Leu Thr Arg Arg Gln Phe Ser Ser Thr Ala Phe Asn           
1               5                   10                  15                

cca tac aag gtc acc gtt cta ggt gct ggt ggt ggt att ggt caa cca         96
Pro Tyr Lys Val Thr Val Leu Gly Ala Gly Gly Gly Ile Gly Gln Pro           
            20                  25                  30                    

ttg tcc ttg ttg ttg aag cta aac cac aag gtc act gac ttg aga cta        144
Leu Ser Leu Leu Leu Lys Leu Asn His Lys Val Thr Asp Leu Arg Leu           
        35                  40                  45                        

tac gac ttg aag ggt gcc aag ggt gtc gct gct gac ttg tct cac atc        192
Tyr Asp Leu Lys Gly Ala Lys Gly Val Ala Ala Asp Leu Ser His Ile           
    50                  55                  60                            

cca acc aac tct acc gtt act ggt tac act cca gaa tcc aag gac tct        240
Pro Thr Asn Ser Thr Val Thr Gly Tyr Thr Pro Glu Ser Lys Asp Ser           
65                  70                  75                  80            

caa gaa gaa ttg gct gct gct ttg aag gac act gag gtt gtt ttg atc        288
Gln Glu Glu Leu Ala Ala Ala Leu Lys Asp Thr Glu Val Val Leu Ile           
                85                  90                  95                

cca gct ggt gtg cca aga aag cca ggt atg acc cgt gac gat ttg ttc        336
Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe           
            100                 105                 110                   

gcc atc aat gcc ggt att gtc aga gat ttg gcc act tcc atc gcc aag        384
Ala Ile Asn Ala Gly Ile Val Arg Asp Leu Ala Thr Ser Ile Ala Lys           
        115                 120                 125                       

aac gct cca aac gcc gcc atc ttg gtc atc tcc aac cca gtc aac tct        432
Asn Ala Pro Asn Ala Ala Ile Leu Val Ile Ser Asn Pro Val Asn Ser           
    130                 135                 140                           

act gtc cca atc gtc gcc gag gtc ttg aag caa aac ggc gtc tac aac        480
Thr Val Pro Ile Val Ala Glu Val Leu Lys Gln Asn Gly Val Tyr Asn           
145                 150                 155                 160           

cca aag aag ttg ttc ggt gtc acc act ttg gac gtt atc cgt gcc tcc        528
Pro Lys Lys Leu Phe Gly Val Thr Thr Leu Asp Val Ile Arg Ala Ser           
                165                 170                 175               

aga ttc atc tcc gag gtt aga ggt acc gac cca acc act gag cac gtg        576
Arg Phe Ile Ser Glu Val Arg Gly Thr Asp Pro Thr Thr Glu His Val           
            180                 185                 190                   

acc gtc gtc ggt ggt cac tcc ggt atc acc atc ttg ccg cta gtg tcc        624
Thr Val Val Gly Gly His Ser Gly Ile Thr Ile Leu Pro Leu Val Ser           
        195                 200                 205                       

cag acc aag cac aag tcc gtc atc aag ggc gag gaa ttg gac aac ttg        672
Gln Thr Lys His Lys Ser Val Ile Lys Gly Glu Glu Leu Asp Asn Leu           
    210                 215                 220                           

atc cac aga atc caa ttc ggt ggt gac gaa gtc gtc cag gca aag aac        720
Ile His Arg Ile Gln Phe Gly Gly Asp Glu Val Val Gln Ala Lys Asn           
225                 230                 235                 240           

ggt gct ggt tct gcc act ttg tcc atg gcc caa gcc ggt gcc cgt ttc        768
Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Phe           
                245                 250                 255               

gct aac agc gtt cta agc ggt ttc gaa ggt gaa aga gac gtc att gag        816
Ala Asn Ser Val Leu Ser Gly Phe Glu Gly Glu Arg Asp Val Ile Glu           
            260                 265                 270                   

cca act ttc gtc gac tcc cca ttg ttc aag gac gaa ggt atc gaa ttc        864
Pro Thr Phe Val Asp Ser Pro Leu Phe Lys Asp Glu Gly Ile Glu Phe           
        275                 280                 285                       

ttc gct tcc cca gtc act ttg ggc cca gaa ggt gtc gaa aag atc cac        912
Phe Ala Ser Pro Val Thr Leu Gly Pro Glu Gly Val Glu Lys Ile His           
    290                 295                 300                           

ggt ttg ggt gtc ttg tcc gac aag gaa gaa caa atg ttg gcc act tgt        960
Gly Leu Gly Val Leu Ser Asp Lys Glu Glu Gln Met Leu Ala Thr Cys           
305                 310                 315                 320           

aag gaa acc ttg aag aag aac atc gaa aag ggt caa aac ttt gtc aag       1008
Lys Glu Thr Leu Lys Lys Asn Ile Glu Lys Gly Gln Asn Phe Val Lys           
                325                 330                 335               

caa aac taa                                                           1017
Gln Asn                                                                   
                                                                          


<210>  20
<211>  338
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  20

Met Leu Arg Ala Leu Thr Arg Arg Gln Phe Ser Ser Thr Ala Phe Asn 
1               5                   10                  15      


Pro Tyr Lys Val Thr Val Leu Gly Ala Gly Gly Gly Ile Gly Gln Pro 
            20                  25                  30          


Leu Ser Leu Leu Leu Lys Leu Asn His Lys Val Thr Asp Leu Arg Leu 
        35                  40                  45              


Tyr Asp Leu Lys Gly Ala Lys Gly Val Ala Ala Asp Leu Ser His Ile 
    50                  55                  60                  


Pro Thr Asn Ser Thr Val Thr Gly Tyr Thr Pro Glu Ser Lys Asp Ser 
65                  70                  75                  80  


Gln Glu Glu Leu Ala Ala Ala Leu Lys Asp Thr Glu Val Val Leu Ile 
                85                  90                  95      


Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe 
            100                 105                 110         


Ala Ile Asn Ala Gly Ile Val Arg Asp Leu Ala Thr Ser Ile Ala Lys 
        115                 120                 125             


Asn Ala Pro Asn Ala Ala Ile Leu Val Ile Ser Asn Pro Val Asn Ser 
    130                 135                 140                 


Thr Val Pro Ile Val Ala Glu Val Leu Lys Gln Asn Gly Val Tyr Asn 
145                 150                 155                 160 


Pro Lys Lys Leu Phe Gly Val Thr Thr Leu Asp Val Ile Arg Ala Ser 
                165                 170                 175     


Arg Phe Ile Ser Glu Val Arg Gly Thr Asp Pro Thr Thr Glu His Val 
            180                 185                 190         


Thr Val Val Gly Gly His Ser Gly Ile Thr Ile Leu Pro Leu Val Ser 
        195                 200                 205             


Gln Thr Lys His Lys Ser Val Ile Lys Gly Glu Glu Leu Asp Asn Leu 
    210                 215                 220                 


Ile His Arg Ile Gln Phe Gly Gly Asp Glu Val Val Gln Ala Lys Asn 
225                 230                 235                 240 


Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Phe 
                245                 250                 255     


Ala Asn Ser Val Leu Ser Gly Phe Glu Gly Glu Arg Asp Val Ile Glu 
            260                 265                 270         


Pro Thr Phe Val Asp Ser Pro Leu Phe Lys Asp Glu Gly Ile Glu Phe 
        275                 280                 285             


Phe Ala Ser Pro Val Thr Leu Gly Pro Glu Gly Val Glu Lys Ile His 
    290                 295                 300                 


Gly Leu Gly Val Leu Ser Asp Lys Glu Glu Gln Met Leu Ala Thr Cys 
305                 310                 315                 320 


Lys Glu Thr Leu Lys Lys Asn Ile Glu Lys Gly Gln Asn Phe Val Lys 
                325                 330                 335     


Gln Asn 
        


<210>  21
<211>  1017
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1017)

<400>  21
atg gtt agc gtt gca gta tta gga tca tcc gga ggc att ggc caa cca         48
Met Val Ser Val Ala Val Leu Gly Ser Ser Gly Gly Ile Gly Gln Pro           
1               5                   10                  15                

ctc tca ctc ttg ttg aag ctg gac cct cgc gtg tcc agc ttg aga ttg         96
Leu Ser Leu Leu Leu Lys Leu Asp Pro Arg Val Ser Ser Leu Arg Leu           
            20                  25                  30                    

tac gac ttg aag atg tcc cac ggg atc gcc acc gat ttg tcg cac atg        144
Tyr Asp Leu Lys Met Ser His Gly Ile Ala Thr Asp Leu Ser His Met           
        35                  40                  45                        

gac tcc aac tcc atc tgc gag ggc ttc aac acc gac gag atc gcg ctc        192
Asp Ser Asn Ser Ile Cys Glu Gly Phe Asn Thr Asp Glu Ile Ala Leu           
    50                  55                  60                            

gcg ctc aag ggc gcc cag atc gtc gtc atc ccc gcg ggt gtc cca aga        240
Ala Leu Lys Gly Ala Gln Ile Val Val Ile Pro Ala Gly Val Pro Arg           
65                  70                  75                  80            

aag ccc ggg atg tca cgt gac gac ctt ttc aag atc aac gcc aag atc        288
Lys Pro Gly Met Ser Arg Asp Asp Leu Phe Lys Ile Asn Ala Lys Ile           
                85                  90                  95                

atc aag tcg ttg gcg ttg caa ata gcc gag cac gcg ccc gag gcg cgc        336
Ile Lys Ser Leu Ala Leu Gln Ile Ala Glu His Ala Pro Glu Ala Arg           
            100                 105                 110                   

gtc ctc gtg atc tcg aac ccg gtc aac tcc ttg gtg ccc att gtg tac        384
Val Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Ile Val Tyr           
        115                 120                 125                       

gag act ttg aag agc gtc ggc aag ttc gag ccg ggt aaa gtg atg gga        432
Glu Thr Leu Lys Ser Val Gly Lys Phe Glu Pro Gly Lys Val Met Gly           
    130                 135                 140                           

att acc aca ttg gac att atc cgc tca cac acg ttc ctg gtg gac gtc        480
Ile Thr Thr Leu Asp Ile Ile Arg Ser His Thr Phe Leu Val Asp Val           
145                 150                 155                 160           

ttg ggc cgc aag gcg tac agc gtc gag aag ttg cgc agc gcg gtt act        528
Leu Gly Arg Lys Ala Tyr Ser Val Glu Lys Leu Arg Ser Ala Val Thr           
                165                 170                 175               

gtg gtg ggc ggc cac tcg ggc gag acc att gtt ccg att ttc acc gac        576
Val Val Gly Gly His Ser Gly Glu Thr Ile Val Pro Ile Phe Thr Asp           
            180                 185                 190                   

cag aag ttc tac agg cgt ctc aga gac aga gag ctc tat gac gcg tac        624
Gln Lys Phe Tyr Arg Arg Leu Arg Asp Arg Glu Leu Tyr Asp Ala Tyr           
        195                 200                 205                       

gtg cat agg gtc caa ttc ggc gga gac gag gtc gtg aag gcc aag gac        672
Val His Arg Val Gln Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp           
    210                 215                 220                           

ggc agc ggt agt gct act ttg tct atg gcc tgg gcg ggt tac agt ttt        720
Gly Ser Gly Ser Ala Thr Leu Ser Met Ala Trp Ala Gly Tyr Ser Phe           
225                 230                 235                 240           

gtg aag cag ttg ctc aac agc ttg cac cta gaa aca ggc gaa gac gtg        768
Val Lys Gln Leu Leu Asn Ser Leu His Leu Glu Thr Gly Glu Asp Val           
                245                 250                 255               

cat ccg atc cca acg ttt gtg tac ttg ccg ggt tta ccg ggc ggg aag        816
His Pro Ile Pro Thr Phe Val Tyr Leu Pro Gly Leu Pro Gly Gly Lys           
            260                 265                 270                   

gag ctc cag cag aag ttg ggc acc tct gtt gag ttt ttt gcc gcg ccc        864
Glu Leu Gln Gln Lys Leu Gly Thr Ser Val Glu Phe Phe Ala Ala Pro           
        275                 280                 285                       

gtg aag ctt tcc aag ggt att gtg gtt gaa gtt gag cac gac tgg gtc        912
Val Lys Leu Ser Lys Gly Ile Val Val Glu Val Glu His Asp Trp Val           
    290                 295                 300                           

gac aag ttg aac gat gcc gag aag aag ttg att gca aag tgt ctt cca        960
Asp Lys Leu Asn Asp Ala Glu Lys Lys Leu Ile Ala Lys Cys Leu Pro           
305                 310                 315                 320           

atc ctt gac aag aac atc aag aag ggt ctc gcc ttt tcg cag cag aca       1008
Ile Leu Asp Lys Asn Ile Lys Lys Gly Leu Ala Phe Ser Gln Gln Thr           
                325                 330                 335               

aag ttg tga                                                           1017
Lys Leu                                                                   
                                                                          


<210>  22
<211>  338
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  22

Met Val Ser Val Ala Val Leu Gly Ser Ser Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Leu Asp Pro Arg Val Ser Ser Leu Arg Leu 
            20                  25                  30          


Tyr Asp Leu Lys Met Ser His Gly Ile Ala Thr Asp Leu Ser His Met 
        35                  40                  45              


Asp Ser Asn Ser Ile Cys Glu Gly Phe Asn Thr Asp Glu Ile Ala Leu 
    50                  55                  60                  


Ala Leu Lys Gly Ala Gln Ile Val Val Ile Pro Ala Gly Val Pro Arg 
65                  70                  75                  80  


Lys Pro Gly Met Ser Arg Asp Asp Leu Phe Lys Ile Asn Ala Lys Ile 
                85                  90                  95      


Ile Lys Ser Leu Ala Leu Gln Ile Ala Glu His Ala Pro Glu Ala Arg 
            100                 105                 110         


Val Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Ile Val Tyr 
        115                 120                 125             


Glu Thr Leu Lys Ser Val Gly Lys Phe Glu Pro Gly Lys Val Met Gly 
    130                 135                 140                 


Ile Thr Thr Leu Asp Ile Ile Arg Ser His Thr Phe Leu Val Asp Val 
145                 150                 155                 160 


Leu Gly Arg Lys Ala Tyr Ser Val Glu Lys Leu Arg Ser Ala Val Thr 
                165                 170                 175     


Val Val Gly Gly His Ser Gly Glu Thr Ile Val Pro Ile Phe Thr Asp 
            180                 185                 190         


Gln Lys Phe Tyr Arg Arg Leu Arg Asp Arg Glu Leu Tyr Asp Ala Tyr 
        195                 200                 205             


Val His Arg Val Gln Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp 
    210                 215                 220                 


Gly Ser Gly Ser Ala Thr Leu Ser Met Ala Trp Ala Gly Tyr Ser Phe 
225                 230                 235                 240 


Val Lys Gln Leu Leu Asn Ser Leu His Leu Glu Thr Gly Glu Asp Val 
                245                 250                 255     


His Pro Ile Pro Thr Phe Val Tyr Leu Pro Gly Leu Pro Gly Gly Lys 
            260                 265                 270         


Glu Leu Gln Gln Lys Leu Gly Thr Ser Val Glu Phe Phe Ala Ala Pro 
        275                 280                 285             


Val Lys Leu Ser Lys Gly Ile Val Val Glu Val Glu His Asp Trp Val 
    290                 295                 300                 


Asp Lys Leu Asn Asp Ala Glu Lys Lys Leu Ile Ala Lys Cys Leu Pro 
305                 310                 315                 320 


Ile Leu Asp Lys Asn Ile Lys Lys Gly Leu Ala Phe Ser Gln Gln Thr 
                325                 330                 335     


Lys Leu 
        


<210>  23
<211>  1125
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1125)

<400>  23
atg cca gca gta tca tat gat gtc cag caa cgg gat atc ctc aag atc         48
Met Pro Ala Val Ser Tyr Asp Val Gln Gln Arg Asp Ile Leu Lys Ile           
1               5                   10                  15                

gca gtt cta ggg gcg gca ggc ggt att ggc caa tcc ttg tcg ctc ttg         96
Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu           
            20                  25                  30                    

ttg aag tcg aac gct tct ttt ttg tta cca cgt gac tcg tca aga cac        144
Leu Lys Ser Asn Ala Ser Phe Leu Leu Pro Arg Asp Ser Ser Arg His           
        35                  40                  45                        

ata agc cta gcg cta tac gac gtg aac aaa gat gcc atc gtg ggc aca        192
Ile Ser Leu Ala Leu Tyr Asp Val Asn Lys Asp Ala Ile Val Gly Thr           
    50                  55                  60                            

gca gca gac ttg tca cac ata gac acc cct atc acc acc act cca cac        240
Ala Ala Asp Leu Ser His Ile Asp Thr Pro Ile Thr Thr Thr Pro His           
65                  70                  75                  80            

tac cca aat gat ggg aat ggc ggt atc gca cgg tgc ttg caa gat gca        288
Tyr Pro Asn Asp Gly Asn Gly Gly Ile Ala Arg Cys Leu Gln Asp Ala           
                85                  90                  95                

gac atg gtc atc atc cca gca ggt gtg ccc aga aaa ccc ggt atg tca        336
Asp Met Val Ile Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Ser           
            100                 105                 110                   

cgt gat gac cta atc ggt gtc aac gcc aag atc atc aag tcg cta gga        384
Arg Asp Asp Leu Ile Gly Val Asn Ala Lys Ile Ile Lys Ser Leu Gly           
        115                 120                 125                       

aac gac atc gca gag tac tgt gac ttg tct aaa gtg cat gta ttg gtt        432
Asn Asp Ile Ala Glu Tyr Cys Asp Leu Ser Lys Val His Val Leu Val           
    130                 135                 140                           

att tcg aac cca gtg aac tcg ttg gtc cca ctg atg gtg tcg act ttg        480
Ile Ser Asn Pro Val Asn Ser Leu Val Pro Leu Met Val Ser Thr Leu           
145                 150                 155                 160           

gca aac agc cca cac agt gcg aac aca aac atc gag gct aga gtg tac        528
Ala Asn Ser Pro His Ser Ala Asn Thr Asn Ile Glu Ala Arg Val Tyr           
                165                 170                 175               

ggg atc acc cat ttg gac cta gtg aga gct tcc acc ttt gtg caa cag        576
Gly Ile Thr His Leu Asp Leu Val Arg Ala Ser Thr Phe Val Gln Gln           
            180                 185                 190                   

cta aac tct ttc aaa tca aat aac gca cct gac att ccg gtc att ggt        624
Leu Asn Ser Phe Lys Ser Asn Asn Ala Pro Asp Ile Pro Val Ile Gly           
        195                 200                 205                       

ggt cat tcc gga gat acc atc atc ccc gtt ttt tcc gtc ttg aat cac        672
Gly His Ser Gly Asp Thr Ile Ile Pro Val Phe Ser Val Leu Asn His           
    210                 215                 220                           

cgc gct tct aac tcc gga tac gct aat ttg cta gat aat ggc gtt agg        720
Arg Ala Ser Asn Ser Gly Tyr Ala Asn Leu Leu Asp Asn Gly Val Arg           
225                 230                 235                 240           

caa aag ttg gtc cac aga gtt caa tat ggt ggg gac gaa atc gtc caa        768
Gln Lys Leu Val His Arg Val Gln Tyr Gly Gly Asp Glu Ile Val Gln           
                245                 250                 255               

gca aag aac ggt aac ggg agc gcg aca tta tcc atg gca tac gcg ggc        816
Ala Lys Asn Gly Asn Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Gly           
            260                 265                 270                   

ttc aaa atc gca gca caa ttc atc gac ctt ttg gtc gga aat atc cgc        864
Phe Lys Ile Ala Ala Gln Phe Ile Asp Leu Leu Val Gly Asn Ile Arg           
        275                 280                 285                       

act atc gaa aat att tgc atg tat gtt ccg ctc act aac agg tat aat        912
Thr Ile Glu Asn Ile Cys Met Tyr Val Pro Leu Thr Asn Arg Tyr Asn           
    290                 295                 300                           

acc gag atc gcc cca ggc tct gac gaa tta aga tca aag tac atc aac        960
Thr Glu Ile Ala Pro Gly Ser Asp Glu Leu Arg Ser Lys Tyr Ile Asn           
305                 310                 315                 320           

gga acc ctt tat ttc tcg att cca ctt tcc atc gga ata aac ggt atc       1008
Gly Thr Leu Tyr Phe Ser Ile Pro Leu Ser Ile Gly Ile Asn Gly Ile           
                325                 330                 335               

gaa aga gtc cac tac gag atc atg gaa cat cta gac agc tac gag cgt       1056
Glu Arg Val His Tyr Glu Ile Met Glu His Leu Asp Ser Tyr Glu Arg           
            340                 345                 350                   

gag acg cta cta ccg atc tgc ttg gaa act cta aag ggt aat att gac       1104
Glu Thr Leu Leu Pro Ile Cys Leu Glu Thr Leu Lys Gly Asn Ile Asp           
        355                 360                 365                       

aag ggt cta agc ttg gta taa                                           1125
Lys Gly Leu Ser Leu Val                                                   
    370                                                                   


<210>  24
<211>  374
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  24

Met Pro Ala Val Ser Tyr Asp Val Gln Gln Arg Asp Ile Leu Lys Ile 
1               5                   10                  15      


Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu 
            20                  25                  30          


Leu Lys Ser Asn Ala Ser Phe Leu Leu Pro Arg Asp Ser Ser Arg His 
        35                  40                  45              


Ile Ser Leu Ala Leu Tyr Asp Val Asn Lys Asp Ala Ile Val Gly Thr 
    50                  55                  60                  


Ala Ala Asp Leu Ser His Ile Asp Thr Pro Ile Thr Thr Thr Pro His 
65                  70                  75                  80  


Tyr Pro Asn Asp Gly Asn Gly Gly Ile Ala Arg Cys Leu Gln Asp Ala 
                85                  90                  95      


Asp Met Val Ile Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Ser 
            100                 105                 110         


Arg Asp Asp Leu Ile Gly Val Asn Ala Lys Ile Ile Lys Ser Leu Gly 
        115                 120                 125             


Asn Asp Ile Ala Glu Tyr Cys Asp Leu Ser Lys Val His Val Leu Val 
    130                 135                 140                 


Ile Ser Asn Pro Val Asn Ser Leu Val Pro Leu Met Val Ser Thr Leu 
145                 150                 155                 160 


Ala Asn Ser Pro His Ser Ala Asn Thr Asn Ile Glu Ala Arg Val Tyr 
                165                 170                 175     


Gly Ile Thr His Leu Asp Leu Val Arg Ala Ser Thr Phe Val Gln Gln 
            180                 185                 190         


Leu Asn Ser Phe Lys Ser Asn Asn Ala Pro Asp Ile Pro Val Ile Gly 
        195                 200                 205             


Gly His Ser Gly Asp Thr Ile Ile Pro Val Phe Ser Val Leu Asn His 
    210                 215                 220                 


Arg Ala Ser Asn Ser Gly Tyr Ala Asn Leu Leu Asp Asn Gly Val Arg 
225                 230                 235                 240 


Gln Lys Leu Val His Arg Val Gln Tyr Gly Gly Asp Glu Ile Val Gln 
                245                 250                 255     


Ala Lys Asn Gly Asn Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Gly 
            260                 265                 270         


Phe Lys Ile Ala Ala Gln Phe Ile Asp Leu Leu Val Gly Asn Ile Arg 
        275                 280                 285             


Thr Ile Glu Asn Ile Cys Met Tyr Val Pro Leu Thr Asn Arg Tyr Asn 
    290                 295                 300                 


Thr Glu Ile Ala Pro Gly Ser Asp Glu Leu Arg Ser Lys Tyr Ile Asn 
305                 310                 315                 320 


Gly Thr Leu Tyr Phe Ser Ile Pro Leu Ser Ile Gly Ile Asn Gly Ile 
                325                 330                 335     


Glu Arg Val His Tyr Glu Ile Met Glu His Leu Asp Ser Tyr Glu Arg 
            340                 345                 350         


Glu Thr Leu Leu Pro Ile Cys Leu Glu Thr Leu Lys Gly Asn Ile Asp 
        355                 360                 365             


Lys Gly Leu Ser Leu Val 
    370                 


<210>  25
<211>  1518
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1518)

<400>  25
atg ggt gtc cag ttt atc gaa aat acc att atc gtt gtc ttt ggt gcg         48
Met Gly Val Gln Phe Ile Glu Asn Thr Ile Ile Val Val Phe Gly Ala           
1               5                   10                  15                

tct gga gat tta gcc aag aag aag act ttc ccc gcc ctg ttt gga cta         96
Ser Gly Asp Leu Ala Lys Lys Lys Thr Phe Pro Ala Leu Phe Gly Leu           
            20                  25                  30                    

ttc agg gag ggc cag ctc tca gaa aca acc aaa atc att ggg ttt gct        144
Phe Arg Glu Gly Gln Leu Ser Glu Thr Thr Lys Ile Ile Gly Phe Ala           
        35                  40                  45                        

cga tca aaa cta tca aat gat gac ttg agg aac aga ata aag ccg tac        192
Arg Ser Lys Leu Ser Asn Asp Asp Leu Arg Asn Arg Ile Lys Pro Tyr           
    50                  55                  60                            

ttg aaa ttg aac aag aga aca gat gct gaa agg cag tct ctg gag aag        240
Leu Lys Leu Asn Lys Arg Thr Asp Ala Glu Arg Gln Ser Leu Glu Lys           
65                  70                  75                  80            

ttt ctg cag att ctc gag tat cac cag tca aac tac gac gac agt gaa        288
Phe Leu Gln Ile Leu Glu Tyr His Gln Ser Asn Tyr Asp Asp Ser Glu           
                85                  90                  95                

ggt ttt gaa aaa ttg gag aag cta atc aat aag tac gac gat gag gca        336
Gly Phe Glu Lys Leu Glu Lys Leu Ile Asn Lys Tyr Asp Asp Glu Ala           
            100                 105                 110                   

aac gtg aaa gag tct cac agg ttg tac tat ttg gct tta cca ccg tct        384
Asn Val Lys Glu Ser His Arg Leu Tyr Tyr Leu Ala Leu Pro Pro Ser           
        115                 120                 125                       

gtc ttt aca acc gtt gca aca atg ttg aaa aaa cat tgt cat cca ggt        432
Val Phe Thr Thr Val Ala Thr Met Leu Lys Lys His Cys His Pro Gly           
    130                 135                 140                           

gat tct ggt att gct agg cta att gtc gag aaa ccc ttt ggc cat gac        480
Asp Ser Gly Ile Ala Arg Leu Ile Val Glu Lys Pro Phe Gly His Asp           
145                 150                 155                 160           

ttg agc tcg tcc cgt gag cta caa aag tct tta gct cca ctt tgg aat        528
Leu Ser Ser Ser Arg Glu Leu Gln Lys Ser Leu Ala Pro Leu Trp Asn           
                165                 170                 175               

gaa gat gaa ttg ttt aga att gat cat tat ttg ggc aaa gaa atg gtt        576
Glu Asp Glu Leu Phe Arg Ile Asp His Tyr Leu Gly Lys Glu Met Val           
            180                 185                 190                   

aag aat tta att cct ttg agg ttt tca aat acg ttt ttg agc agt tct        624
Lys Asn Leu Ile Pro Leu Arg Phe Ser Asn Thr Phe Leu Ser Ser Ser           
        195                 200                 205                       

tgg aac aat caa ttt att gac acc atc caa atc act ttt aag gag aac        672
Trp Asn Asn Gln Phe Ile Asp Thr Ile Gln Ile Thr Phe Lys Glu Asn           
    210                 215                 220                           

ttt gga act gaa gga cgt ggt ggt tac ttt gat tcc att ggt ata ata        720
Phe Gly Thr Glu Gly Arg Gly Gly Tyr Phe Asp Ser Ile Gly Ile Ile           
225                 230                 235                 240           

aga gat gtt atc caa aat cat ttg tta caa gtc ttg act att gtt ttg        768
Arg Asp Val Ile Gln Asn His Leu Leu Gln Val Leu Thr Ile Val Leu           
                245                 250                 255               

atg gaa aaa cca gcg gat ttt aat gga gaa tct atc aga gat gaa aag        816
Met Glu Lys Pro Ala Asp Phe Asn Gly Glu Ser Ile Arg Asp Glu Lys           
            260                 265                 270                   

gtt aaa gtg tta aag gca att gaa caa att gat ttc aat aat gtg ttg        864
Val Lys Val Leu Lys Ala Ile Glu Gln Ile Asp Phe Asn Asn Val Leu           
        275                 280                 285                       

gta ggt caa tat gat aaa tct gaa gat ggt agt aaa cct ggt tac ttg        912
Val Gly Gln Tyr Asp Lys Ser Glu Asp Gly Ser Lys Pro Gly Tyr Leu           
    290                 295                 300                           

gat gat gat acc gtc aat cca gat tct aaa gct gtc act tat gct gcc        960
Asp Asp Asp Thr Val Asn Pro Asp Ser Lys Ala Val Thr Tyr Ala Ala           
305                 310                 315                 320           

tta gtt tta aat gtg gca aac gaa aga tgg aat aat gtt ccg atc att       1008
Leu Val Leu Asn Val Ala Asn Glu Arg Trp Asn Asn Val Pro Ile Ile           
                325                 330                 335               

cta aag gca ggc aag gcc ttg aat caa tcc aag gtg gaa att aga atc       1056
Leu Lys Ala Gly Lys Ala Leu Asn Gln Ser Lys Val Glu Ile Arg Ile           
            340                 345                 350                   

cag ttc aaa cca gta gaa aat gga atc ttc aaa aac tct gct agg aat       1104
Gln Phe Lys Pro Val Glu Asn Gly Ile Phe Lys Asn Ser Ala Arg Asn           
        355                 360                 365                       

gag ttg gtt att agg atc caa cca aac gag gca atg tat ttg aaa atg       1152
Glu Leu Val Ile Arg Ile Gln Pro Asn Glu Ala Met Tyr Leu Lys Met           
    370                 375                 380                           

aac atc aaa gta cct ggt gtt tcc aat caa gtg tcg att tca gaa atg       1200
Asn Ile Lys Val Pro Gly Val Ser Asn Gln Val Ser Ile Ser Glu Met           
385                 390                 395                 400           

gat ttg act tac aag aat agg tat tcc tcc gaa ttt tac att cca gaa       1248
Asp Leu Thr Tyr Lys Asn Arg Tyr Ser Ser Glu Phe Tyr Ile Pro Glu           
                405                 410                 415               

gct tat gaa tct ttg att aaa gat gcc tta atg gat gat cat tca aat       1296
Ala Tyr Glu Ser Leu Ile Lys Asp Ala Leu Met Asp Asp His Ser Asn           
            420                 425                 430                   

ttt gtt aga gac gat gaa ttg gac att tca tgg gct ttg ttc act cca       1344
Phe Val Arg Asp Asp Glu Leu Asp Ile Ser Trp Ala Leu Phe Thr Pro           
        435                 440                 445                       

tta cta gaa cat atc gaa ggc ccc gat ggt cca act cca acc aag tat       1392
Leu Leu Glu His Ile Glu Gly Pro Asp Gly Pro Thr Pro Thr Lys Tyr           
    450                 455                 460                           

cct tac ggt tcc aga ggt cca aag gag att gac gaa ttt ttg aga aac       1440
Pro Tyr Gly Ser Arg Gly Pro Lys Glu Ile Asp Glu Phe Leu Arg Asn           
465                 470                 475                 480           

cat ggt tat gta aag gaa cca aga gaa aat tac caa tgg cca tta act       1488
His Gly Tyr Val Lys Glu Pro Arg Glu Asn Tyr Gln Trp Pro Leu Thr           
                485                 490                 495               

act cct aaa gaa ttg aac agt tca aag ttt                               1518
Thr Pro Lys Glu Leu Asn Ser Ser Lys Phe                                   
            500                 505                                       


<210>  26
<211>  506
<212>  PRT
<213>  Issatchenkia orientalis

<400>  26

Met Gly Val Gln Phe Ile Glu Asn Thr Ile Ile Val Val Phe Gly Ala 
1               5                   10                  15      


Ser Gly Asp Leu Ala Lys Lys Lys Thr Phe Pro Ala Leu Phe Gly Leu 
            20                  25                  30          


Phe Arg Glu Gly Gln Leu Ser Glu Thr Thr Lys Ile Ile Gly Phe Ala 
        35                  40                  45              


Arg Ser Lys Leu Ser Asn Asp Asp Leu Arg Asn Arg Ile Lys Pro Tyr 
    50                  55                  60                  


Leu Lys Leu Asn Lys Arg Thr Asp Ala Glu Arg Gln Ser Leu Glu Lys 
65                  70                  75                  80  


Phe Leu Gln Ile Leu Glu Tyr His Gln Ser Asn Tyr Asp Asp Ser Glu 
                85                  90                  95      


Gly Phe Glu Lys Leu Glu Lys Leu Ile Asn Lys Tyr Asp Asp Glu Ala 
            100                 105                 110         


Asn Val Lys Glu Ser His Arg Leu Tyr Tyr Leu Ala Leu Pro Pro Ser 
        115                 120                 125             


Val Phe Thr Thr Val Ala Thr Met Leu Lys Lys His Cys His Pro Gly 
    130                 135                 140                 


Asp Ser Gly Ile Ala Arg Leu Ile Val Glu Lys Pro Phe Gly His Asp 
145                 150                 155                 160 


Leu Ser Ser Ser Arg Glu Leu Gln Lys Ser Leu Ala Pro Leu Trp Asn 
                165                 170                 175     


Glu Asp Glu Leu Phe Arg Ile Asp His Tyr Leu Gly Lys Glu Met Val 
            180                 185                 190         


Lys Asn Leu Ile Pro Leu Arg Phe Ser Asn Thr Phe Leu Ser Ser Ser 
        195                 200                 205             


Trp Asn Asn Gln Phe Ile Asp Thr Ile Gln Ile Thr Phe Lys Glu Asn 
    210                 215                 220                 


Phe Gly Thr Glu Gly Arg Gly Gly Tyr Phe Asp Ser Ile Gly Ile Ile 
225                 230                 235                 240 


Arg Asp Val Ile Gln Asn His Leu Leu Gln Val Leu Thr Ile Val Leu 
                245                 250                 255     


Met Glu Lys Pro Ala Asp Phe Asn Gly Glu Ser Ile Arg Asp Glu Lys 
            260                 265                 270         


Val Lys Val Leu Lys Ala Ile Glu Gln Ile Asp Phe Asn Asn Val Leu 
        275                 280                 285             


Val Gly Gln Tyr Asp Lys Ser Glu Asp Gly Ser Lys Pro Gly Tyr Leu 
    290                 295                 300                 


Asp Asp Asp Thr Val Asn Pro Asp Ser Lys Ala Val Thr Tyr Ala Ala 
305                 310                 315                 320 


Leu Val Leu Asn Val Ala Asn Glu Arg Trp Asn Asn Val Pro Ile Ile 
                325                 330                 335     


Leu Lys Ala Gly Lys Ala Leu Asn Gln Ser Lys Val Glu Ile Arg Ile 
            340                 345                 350         


Gln Phe Lys Pro Val Glu Asn Gly Ile Phe Lys Asn Ser Ala Arg Asn 
        355                 360                 365             


Glu Leu Val Ile Arg Ile Gln Pro Asn Glu Ala Met Tyr Leu Lys Met 
    370                 375                 380                 


Asn Ile Lys Val Pro Gly Val Ser Asn Gln Val Ser Ile Ser Glu Met 
385                 390                 395                 400 


Asp Leu Thr Tyr Lys Asn Arg Tyr Ser Ser Glu Phe Tyr Ile Pro Glu 
                405                 410                 415     


Ala Tyr Glu Ser Leu Ile Lys Asp Ala Leu Met Asp Asp His Ser Asn 
            420                 425                 430         


Phe Val Arg Asp Asp Glu Leu Asp Ile Ser Trp Ala Leu Phe Thr Pro 
        435                 440                 445             


Leu Leu Glu His Ile Glu Gly Pro Asp Gly Pro Thr Pro Thr Lys Tyr 
    450                 455                 460                 


Pro Tyr Gly Ser Arg Gly Pro Lys Glu Ile Asp Glu Phe Leu Arg Asn 
465                 470                 475                 480 


His Gly Tyr Val Lys Glu Pro Arg Glu Asn Tyr Gln Trp Pro Leu Thr 
                485                 490                 495     


Thr Pro Lys Glu Leu Asn Ser Ser Lys Phe 
            500                 505     


<210>  27
<211>  759
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(759)

<400>  27
atg gtc aag gtt tat tca tat tcc aac aat gat gag ata gct caa tca         48
Met Val Lys Val Tyr Ser Tyr Ser Asn Asn Asp Glu Ile Ala Gln Ser           
1               5                   10                  15                

ttg gca aag ttt ata gtt tca caa cag gat tcg gtt ctt gca acc aag         96
Leu Ala Lys Phe Ile Val Ser Gln Gln Asp Ser Val Leu Ala Thr Lys           
            20                  25                  30                    

gag aaa ttc aac att gct att agc ggt ggt tca cta ata ggt att tta        144
Glu Lys Phe Asn Ile Ala Ile Ser Gly Gly Ser Leu Ile Gly Ile Leu           
        35                  40                  45                        

ggg aaa ggc tta ctg aac aac aaa gat atc aaa tgg gat aag tgg gta        192
Gly Lys Gly Leu Leu Asn Asn Lys Asp Ile Lys Trp Asp Lys Trp Val           
    50                  55                  60                            

atc tat ttc agt gat gag aga att gta cca ttg agt gac aac gat tcc        240
Ile Tyr Phe Ser Asp Glu Arg Ile Val Pro Leu Ser Asp Asn Asp Ser           
65                  70                  75                  80            

aat ttt ggt gct ttt gaa aag gag gtt ttg gag aaa ctc gcc cat gca        288
Asn Phe Gly Ala Phe Glu Lys Glu Val Leu Glu Lys Leu Ala His Ala           
                85                  90                  95                

ggg aaa gtt ggg cca acg gtt gtt aca atc aac gaa aat tta att cat        336
Gly Lys Val Gly Pro Thr Val Val Thr Ile Asn Glu Asn Leu Ile His           
            100                 105                 110                   

ccg gat gat cac act acg gat ggt gag att gcc cag aca tat gca tct        384
Pro Asp Asp His Thr Thr Asp Gly Glu Ile Ala Gln Thr Tyr Ala Ser           
        115                 120                 125                       

gag ttg cct gaa tcc gga ctg gat ttg gtc ttg cta ggg tgt gga cca        432
Glu Leu Pro Glu Ser Gly Leu Asp Leu Val Leu Leu Gly Cys Gly Pro           
    130                 135                 140                           

gac ggc cac aca tgt tct ttg ttc cca ggc cac aag ttg ttg gag gag        480
Asp Gly His Thr Cys Ser Leu Phe Pro Gly His Lys Leu Leu Glu Glu           
145                 150                 155                 160           

aac gag ctt gat gtt gct gcg ctt cat gat tct cca aag cca cca cca        528
Asn Glu Leu Asp Val Ala Ala Leu His Asp Ser Pro Lys Pro Pro Pro           
                165                 170                 175               

agg aga atc aca ttg aca ttc aag tat ctt gct aaa tgt act aca cta        576
Arg Arg Ile Thr Leu Thr Phe Lys Tyr Leu Ala Lys Cys Thr Thr Leu           
            180                 185                 190                   

gct ttt gtt gcc act ggt gca agc aag cag gaa gct cta aaa gag att        624
Ala Phe Val Ala Thr Gly Ala Ser Lys Gln Glu Ala Leu Lys Glu Ile           
        195                 200                 205                       

ttt gga aac gaa aac agc caa cta ccc tgt gct att act aac agg ttg        672
Phe Gly Asn Glu Asn Ser Gln Leu Pro Cys Ala Ile Thr Asn Arg Leu           
    210                 215                 220                           

gtt tca aaa gtt aag ggg ggt atc tgt tgg ttt gta gat gat gat gcc        720
Val Ser Lys Val Lys Gly Gly Ile Cys Trp Phe Val Asp Asp Asp Ala           
225                 230                 235                 240           

att gaa ggc gtc gac gtc caa acc ctc aag tac tga ttc                    759
Ile Glu Gly Val Asp Val Gln Thr Leu Lys Tyr     Phe                       
                245                 250                                   


<210>  28
<211>  251
<212>  PRT
<213>  Issatchenkia orientalis

<400>  28

Met Val Lys Val Tyr Ser Tyr Ser Asn Asn Asp Glu Ile Ala Gln Ser 
1               5                   10                  15      


Leu Ala Lys Phe Ile Val Ser Gln Gln Asp Ser Val Leu Ala Thr Lys 
            20                  25                  30          


Glu Lys Phe Asn Ile Ala Ile Ser Gly Gly Ser Leu Ile Gly Ile Leu 
        35                  40                  45              


Gly Lys Gly Leu Leu Asn Asn Lys Asp Ile Lys Trp Asp Lys Trp Val 
    50                  55                  60                  


Ile Tyr Phe Ser Asp Glu Arg Ile Val Pro Leu Ser Asp Asn Asp Ser 
65                  70                  75                  80  


Asn Phe Gly Ala Phe Glu Lys Glu Val Leu Glu Lys Leu Ala His Ala 
                85                  90                  95      


Gly Lys Val Gly Pro Thr Val Val Thr Ile Asn Glu Asn Leu Ile His 
            100                 105                 110         


Pro Asp Asp His Thr Thr Asp Gly Glu Ile Ala Gln Thr Tyr Ala Ser 
        115                 120                 125             


Glu Leu Pro Glu Ser Gly Leu Asp Leu Val Leu Leu Gly Cys Gly Pro 
    130                 135                 140                 


Asp Gly His Thr Cys Ser Leu Phe Pro Gly His Lys Leu Leu Glu Glu 
145                 150                 155                 160 


Asn Glu Leu Asp Val Ala Ala Leu His Asp Ser Pro Lys Pro Pro Pro 
                165                 170                 175     


Arg Arg Ile Thr Leu Thr Phe Lys Tyr Leu Ala Lys Cys Thr Thr Leu 
            180                 185                 190         


Ala Phe Val Ala Thr Gly Ala Ser Lys Gln Glu Ala Leu Lys Glu Ile 
        195                 200                 205             


Phe Gly Asn Glu Asn Ser Gln Leu Pro Cys Ala Ile Thr Asn Arg Leu 
    210                 215                 220                 


Val Ser Lys Val Lys Gly Gly Ile Cys Trp Phe Val Asp Asp Asp Ala 
225                 230                 235                 240 


Ile Glu Gly Val Asp Val Gln Thr Leu Lys Tyr 
                245                 250     


<210>  29
<211>  1431
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1431)

<400>  29
atg ggt caa aac ttg att ctt aat gca gca gat cat ggt ttt act gtt         48
Met Gly Gln Asn Leu Ile Leu Asn Ala Ala Asp His Gly Phe Thr Val           
1               5                   10                  15                

gtt gca tac aac aga act gtc tct aaa gtt gac cat ttc ctt caa aat         96
Val Ala Tyr Asn Arg Thr Val Ser Lys Val Asp His Phe Leu Gln Asn           
            20                  25                  30                    

gaa gca aag ggt aaa tcc att att ggt gca cac tcc att gaa gaa tta        144
Glu Ala Lys Gly Lys Ser Ile Ile Gly Ala His Ser Ile Glu Glu Leu           
        35                  40                  45                        

tgt gct aag ttg aag aaa cca aga aga att atg ttg tta gtc aag gca        192
Cys Ala Lys Leu Lys Lys Pro Arg Arg Ile Met Leu Leu Val Lys Ala           
    50                  55                  60                            

ggt aat cca gtt gat caa ttc att gaa caa ttg tta cct cat tta gat        240
Gly Asn Pro Val Asp Gln Phe Ile Glu Gln Leu Leu Pro His Leu Asp           
65                  70                  75                  80            

gaa ggc gat atc att att gac ggt ggt aac tct cac ttc cct gac tcc        288
Glu Gly Asp Ile Ile Ile Asp Gly Gly Asn Ser His Phe Pro Asp Ser           
                85                  90                  95                

aac aga aga tac gag gaa tta aag aag aag ggt att ctc ttt gtc ggt        336
Asn Arg Arg Tyr Glu Glu Leu Lys Lys Lys Gly Ile Leu Phe Val Gly           
            100                 105                 110                   

tct ggt gtt tct ggt ggt gaa gaa ggt gca aga tat ggt cct tct ttg        384
Ser Gly Val Ser Gly Gly Glu Glu Gly Ala Arg Tyr Gly Pro Ser Leu           
        115                 120                 125                       

atg cct ggt ggt gca aag gaa gca tgg cct cat att aag gac atc ttc        432
Met Pro Gly Gly Ala Lys Glu Ala Trp Pro His Ile Lys Asp Ile Phe           
    130                 135                 140                           

caa tct atc tct gca aag gcc gat ggt gag cca tgt tgt gat tgg gtt        480
Gln Ser Ile Ser Ala Lys Ala Asp Gly Glu Pro Cys Cys Asp Trp Val           
145                 150                 155                 160           

ggt gat gca ggt gca ggt cat tac gtt aag atg gtc cac aat ggt atc        528
Gly Asp Ala Gly Ala Gly His Tyr Val Lys Met Val His Asn Gly Ile           
                165                 170                 175               

gag tat ggt gat atg cag ttg atc tgt gaa gct tac gat ttg atg aag        576
Glu Tyr Gly Asp Met Gln Leu Ile Cys Glu Ala Tyr Asp Leu Met Lys           
            180                 185                 190                   

aga gtt ggt ggt tta act gac aag gaa ata tct gat gtt ttc ggt gaa        624
Arg Val Gly Gly Leu Thr Asp Lys Glu Ile Ser Asp Val Phe Gly Glu           
        195                 200                 205                       

tgg aac gag ggt gtt ctc gat tct ttc tta gtt gaa att acc aga gat        672
Trp Asn Glu Gly Val Leu Asp Ser Phe Leu Val Glu Ile Thr Arg Asp           
    210                 215                 220                           

atc tta gct ttc aac gat aag gat ggt acc cca tta gtt gaa aag atc        720
Ile Leu Ala Phe Asn Asp Lys Asp Gly Thr Pro Leu Val Glu Lys Ile           
225                 230                 235                 240           

tta gat act gcc gga cag aag ggt act ggt aaa tgg act gca ata aat        768
Leu Asp Thr Ala Gly Gln Lys Gly Thr Gly Lys Trp Thr Ala Ile Asn           
                245                 250                 255               

gct tta gac ttg ggt atg cca gtc act tta att ggt gaa gct gtt ttt        816
Ala Leu Asp Leu Gly Met Pro Val Thr Leu Ile Gly Glu Ala Val Phe           
            260                 265                 270                   

gcg aga tgt tta tcc gct ttg aag cca gaa aga gag aga gct tct gaa        864
Ala Arg Cys Leu Ser Ala Leu Lys Pro Glu Arg Glu Arg Ala Ser Glu           
        275                 280                 285                       

atc tta aac ggt ccg gaa gtt gaa caa gtt tct gct gaa ggt aga gca        912
Ile Leu Asn Gly Pro Glu Val Glu Gln Val Ser Ala Glu Gly Arg Ala           
    290                 295                 300                           

caa ttt att gca gat ttg atg caa gct tta tat gca tca aag att att        960
Gln Phe Ile Ala Asp Leu Met Gln Ala Leu Tyr Ala Ser Lys Ile Ile           
305                 310                 315                 320           

tct tac gca caa ggt ttc atg tta atc aga gaa gca gca aag gaa tac       1008
Ser Tyr Ala Gln Gly Phe Met Leu Ile Arg Glu Ala Ala Lys Glu Tyr           
                325                 330                 335               

aac tgg aaa tta aac ttc cct tct att gca ctt atg tgg aga ggt ggt       1056
Asn Trp Lys Leu Asn Phe Pro Ser Ile Ala Leu Met Trp Arg Gly Gly           
            340                 345                 350                   

tgt att atc agg tct gtt ttc ttg gct gaa att act gca gct tat agg       1104
Cys Ile Ile Arg Ser Val Phe Leu Ala Glu Ile Thr Ala Ala Tyr Arg           
        355                 360                 365                       

gaa aac cct gac tta gag aac tta cta ttc aac aag ttc ttc caa gat       1152
Glu Asn Pro Asp Leu Glu Asn Leu Leu Phe Asn Lys Phe Phe Gln Asp           
    370                 375                 380                           

gct att cat aag gca cag tct ggt tgg aga aag act gtt gca tta gct       1200
Ala Ile His Lys Ala Gln Ser Gly Trp Arg Lys Thr Val Ala Leu Ala           
385                 390                 395                 400           

gtt acc caa ggt att cca act cca gca ttc tct act gca ttg tct ttc       1248
Val Thr Gln Gly Ile Pro Thr Pro Ala Phe Ser Thr Ala Leu Ser Phe           
                405                 410                 415               

tac gat ggt tac aga tcc aag aag tta cca gct aac ttg ttg caa gca       1296
Tyr Asp Gly Tyr Arg Ser Lys Lys Leu Pro Ala Asn Leu Leu Gln Ala           
            420                 425                 430                   

caa aga gat tac ttc ggt gct cac act ttc caa att tta cct gaa tgt       1344
Gln Arg Asp Tyr Phe Gly Ala His Thr Phe Gln Ile Leu Pro Glu Cys           
        435                 440                 445                       

gca gat gac gaa aag aag gtt ggt gat tac atc cat gtc aac tgg act       1392
Ala Asp Asp Glu Lys Lys Val Gly Asp Tyr Ile His Val Asn Trp Thr           
    450                 455                 460                           

ggt aag ggt ggt aat gtt tct gct agt act tac gat gct                   1431
Gly Lys Gly Gly Asn Val Ser Ala Ser Thr Tyr Asp Ala                       
465                 470                 475                               


<210>  30
<211>  477
<212>  PRT
<213>  Issatchenkia orientalis

<400>  30

Met Gly Gln Asn Leu Ile Leu Asn Ala Ala Asp His Gly Phe Thr Val 
1               5                   10                  15      


Val Ala Tyr Asn Arg Thr Val Ser Lys Val Asp His Phe Leu Gln Asn 
            20                  25                  30          


Glu Ala Lys Gly Lys Ser Ile Ile Gly Ala His Ser Ile Glu Glu Leu 
        35                  40                  45              


Cys Ala Lys Leu Lys Lys Pro Arg Arg Ile Met Leu Leu Val Lys Ala 
    50                  55                  60                  


Gly Asn Pro Val Asp Gln Phe Ile Glu Gln Leu Leu Pro His Leu Asp 
65                  70                  75                  80  


Glu Gly Asp Ile Ile Ile Asp Gly Gly Asn Ser His Phe Pro Asp Ser 
                85                  90                  95      


Asn Arg Arg Tyr Glu Glu Leu Lys Lys Lys Gly Ile Leu Phe Val Gly 
            100                 105                 110         


Ser Gly Val Ser Gly Gly Glu Glu Gly Ala Arg Tyr Gly Pro Ser Leu 
        115                 120                 125             


Met Pro Gly Gly Ala Lys Glu Ala Trp Pro His Ile Lys Asp Ile Phe 
    130                 135                 140                 


Gln Ser Ile Ser Ala Lys Ala Asp Gly Glu Pro Cys Cys Asp Trp Val 
145                 150                 155                 160 


Gly Asp Ala Gly Ala Gly His Tyr Val Lys Met Val His Asn Gly Ile 
                165                 170                 175     


Glu Tyr Gly Asp Met Gln Leu Ile Cys Glu Ala Tyr Asp Leu Met Lys 
            180                 185                 190         


Arg Val Gly Gly Leu Thr Asp Lys Glu Ile Ser Asp Val Phe Gly Glu 
        195                 200                 205             


Trp Asn Glu Gly Val Leu Asp Ser Phe Leu Val Glu Ile Thr Arg Asp 
    210                 215                 220                 


Ile Leu Ala Phe Asn Asp Lys Asp Gly Thr Pro Leu Val Glu Lys Ile 
225                 230                 235                 240 


Leu Asp Thr Ala Gly Gln Lys Gly Thr Gly Lys Trp Thr Ala Ile Asn 
                245                 250                 255     


Ala Leu Asp Leu Gly Met Pro Val Thr Leu Ile Gly Glu Ala Val Phe 
            260                 265                 270         


Ala Arg Cys Leu Ser Ala Leu Lys Pro Glu Arg Glu Arg Ala Ser Glu 
        275                 280                 285             


Ile Leu Asn Gly Pro Glu Val Glu Gln Val Ser Ala Glu Gly Arg Ala 
    290                 295                 300                 


Gln Phe Ile Ala Asp Leu Met Gln Ala Leu Tyr Ala Ser Lys Ile Ile 
305                 310                 315                 320 


Ser Tyr Ala Gln Gly Phe Met Leu Ile Arg Glu Ala Ala Lys Glu Tyr 
                325                 330                 335     


Asn Trp Lys Leu Asn Phe Pro Ser Ile Ala Leu Met Trp Arg Gly Gly 
            340                 345                 350         


Cys Ile Ile Arg Ser Val Phe Leu Ala Glu Ile Thr Ala Ala Tyr Arg 
        355                 360                 365             


Glu Asn Pro Asp Leu Glu Asn Leu Leu Phe Asn Lys Phe Phe Gln Asp 
    370                 375                 380                 


Ala Ile His Lys Ala Gln Ser Gly Trp Arg Lys Thr Val Ala Leu Ala 
385                 390                 395                 400 


Val Thr Gln Gly Ile Pro Thr Pro Ala Phe Ser Thr Ala Leu Ser Phe 
                405                 410                 415     


Tyr Asp Gly Tyr Arg Ser Lys Lys Leu Pro Ala Asn Leu Leu Gln Ala 
            420                 425                 430         


Gln Arg Asp Tyr Phe Gly Ala His Thr Phe Gln Ile Leu Pro Glu Cys 
        435                 440                 445             


Ala Asp Asp Glu Lys Lys Val Gly Asp Tyr Ile His Val Asn Trp Thr 
    450                 455                 460                 


Gly Lys Gly Gly Asn Val Ser Ala Ser Thr Tyr Asp Ala 
465                 470                 475         


<210>  31
<211>  3761
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1037)..(2764)

<400>  31
aaagatacag gtggctcagc tgttgcaggt gtacttctag tatatggaat tgacggctca       60

tcaacattgc ttaatcggcg gcgctttaaa gggttgttag tagtgtctat agtgtctata      120

gtgtctatag tatctgtatt gttgttgttg tttttgggat tattggtgtt aagactctca      180

gtaagtttcg gacgcagatc tgtgttgaag agacgtacaa gatccgcctt tctgtccctt      240

ggcgacgccc tgatcccatg gcttgccaac agcgggcgaa gatccttcac ccgcagcgtc      300

catggattga actcgggcga taggtactgt gacgagtcca tcggttcctg tcagatggga      360

tactcttgac gtggaaaatt caaacagaaa aaaaacccca ataatgaaaa ataacactac      420

gttatatccg tggtatcctc tatcgtatcg tatcgtagcg tatcgtagcg taccgtatca      480

cagtatagtc taatattccg tatcttattg tatcctatcc tattcgatcc tattgtattt      540

ctgtgcacca ttttaatttc tattgctata atgtccttat tagttgccac tgtgaggtga      600

ccaatggacg agggcgagcc gttcagaagc cgcgaagggt gttcttccca tgaatttctt      660

aaggagggcg gctcagctcc gagagtgagg cgagacgtct cggtcagcgt atcccccttc      720

ctcggctttt acaaatgatg cgctcttaat agtgtgtcgt tatccttttg gcattgacgg      780

gggagggaaa ttgattgagc gcatccatat ttttgcggac tgctgaggac aatggtggtt      840

tttccgggtg gcgtgggcta caaatgatac gatggttttt ttcttttcgg agaaggcgta      900

taaaaaggac acggagaacc catttattct aataacagtt gagcttcttt aattatttgt      960

taatataata ttctattatt atatattttc ttcccaataa aacaaaataa aacaaaacac     1020

agcaaaacac aaaaat atg act gac aaa atc tcc cta ggt act tat ctg ttt     1072
                  Met Thr Asp Lys Ile Ser Leu Gly Thr Tyr Leu Phe         
                  1               5                   10                  

gaa aag tta aag gaa gca ggc tct tat tcc atc ttt ggt gtt cct ggt       1120
Glu Lys Leu Lys Glu Ala Gly Ser Tyr Ser Ile Phe Gly Val Pro Gly           
        15                  20                  25                        

gat ttc aat ttg gca ttg ttg gac cac gtc aag gaa gtt gaa ggc att       1168
Asp Phe Asn Leu Ala Leu Leu Asp His Val Lys Glu Val Glu Gly Ile           
    30                  35                  40                            

aga tgg gtc ggt aac gct aac gag ttg aat gcc ggc tac gaa gct gat       1216
Arg Trp Val Gly Asn Ala Asn Glu Leu Asn Ala Gly Tyr Glu Ala Asp           
45                  50                  55                  60            

ggt tat gca aga atc aat gga ttt gca tcc cta atc acc acc ttt ggt       1264
Gly Tyr Ala Arg Ile Asn Gly Phe Ala Ser Leu Ile Thr Thr Phe Gly           
                65                  70                  75                

gtc ggt gaa ttg tct gcc gtc aat gcc att gca ggt tct tat gct gaa       1312
Val Gly Glu Leu Ser Ala Val Asn Ala Ile Ala Gly Ser Tyr Ala Glu           
            80                  85                  90                    

cac gtc cca ttg atc cat att gtt ggt atg cct tcc ttg tct gct atg       1360
His Val Pro Leu Ile His Ile Val Gly Met Pro Ser Leu Ser Ala Met           
        95                  100                 105                       

aag aac aac ttg ttg tta cac cat acc ttg ggt gac aca aga ttc gac       1408
Lys Asn Asn Leu Leu Leu His His Thr Leu Gly Asp Thr Arg Phe Asp           
    110                 115                 120                           

aac ttc acc gaa atg tca aag aaa atc agt gca aag gtt gaa att gtt       1456
Asn Phe Thr Glu Met Ser Lys Lys Ile Ser Ala Lys Val Glu Ile Val           
125                 130                 135                 140           

tac gat ttg gaa tca gct cca aaa tta att aat aac ttg att gaa acc       1504
Tyr Asp Leu Glu Ser Ala Pro Lys Leu Ile Asn Asn Leu Ile Glu Thr           
                145                 150                 155               

gct tat cac aca aag aga cca gtc tac ttg gga ctt cct tcc aac ttt       1552
Ala Tyr His Thr Lys Arg Pro Val Tyr Leu Gly Leu Pro Ser Asn Phe           
            160                 165                 170                   

gct gat gaa ttg gtt cca gcg gca tta gtt aag gaa aac aag tta cat       1600
Ala Asp Glu Leu Val Pro Ala Ala Leu Val Lys Glu Asn Lys Leu His           
        175                 180                 185                       

tta gaa gaa cct cta aac aac ccc gtt gct gaa gaa gaa ttc att cat       1648
Leu Glu Glu Pro Leu Asn Asn Pro Val Ala Glu Glu Glu Phe Ile His           
    190                 195                 200                           

aac gtt gtt gaa atg gtc aag aag gca gaa aaa cca atc att ctc gtt       1696
Asn Val Val Glu Met Val Lys Lys Ala Glu Lys Pro Ile Ile Leu Val           
205                 210                 215                 220           

gac gct tgt gct gca aga cat aac att tct aag gaa gtg aga gag ttg       1744
Asp Ala Cys Ala Ala Arg His Asn Ile Ser Lys Glu Val Arg Glu Leu           
                225                 230                 235               

gct aaa ttg act aaa ttc cct gtc ttc acc acc cca atg ggt aaa tct       1792
Ala Lys Leu Thr Lys Phe Pro Val Phe Thr Thr Pro Met Gly Lys Ser           
            240                 245                 250                   

act gtt gat gaa gat gat gaa gaa ttc ttt ggc tta tac ttg ggt tct       1840
Thr Val Asp Glu Asp Asp Glu Glu Phe Phe Gly Leu Tyr Leu Gly Ser           
        255                 260                 265                       

cta tct gct cca gat gtt aag gac att gtt ggc cca acc gat tgt atc       1888
Leu Ser Ala Pro Asp Val Lys Asp Ile Val Gly Pro Thr Asp Cys Ile           
    270                 275                 280                           

tta tcc tta ggt ggt tta cct tct gat ttc aac acc ggt tcc ttc tca       1936
Leu Ser Leu Gly Gly Leu Pro Ser Asp Phe Asn Thr Gly Ser Phe Ser           
285                 290                 295                 300           

tat ggt tac acc act aag aat gtc gtt gaa ttc cat tcc aac tac tgt       1984
Tyr Gly Tyr Thr Thr Lys Asn Val Val Glu Phe His Ser Asn Tyr Cys           
                305                 310                 315               

aaa ttc aaa tct gca act tat gaa aac ttg atg atg aag ggc gca gtc       2032
Lys Phe Lys Ser Ala Thr Tyr Glu Asn Leu Met Met Lys Gly Ala Val           
            320                 325                 330                   

caa aga ttg atc agc gaa ttg aag aat att aag tat tcc aat gtc tca       2080
Gln Arg Leu Ile Ser Glu Leu Lys Asn Ile Lys Tyr Ser Asn Val Ser           
        335                 340                 345                       

act tta tct cca cca aaa tct aaa ttt gct tac gaa tct gca aag gtt       2128
Thr Leu Ser Pro Pro Lys Ser Lys Phe Ala Tyr Glu Ser Ala Lys Val           
    350                 355                 360                           

gct cca gaa ggt atc atc act caa gat tac ctg tgg aag aga tta tct       2176
Ala Pro Glu Gly Ile Ile Thr Gln Asp Tyr Leu Trp Lys Arg Leu Ser           
365                 370                 375                 380           

tac ttc tta aag cca aga gat atc att gtc act gaa act ggt act tcc       2224
Tyr Phe Leu Lys Pro Arg Asp Ile Ile Val Thr Glu Thr Gly Thr Ser           
                385                 390                 395               

tcc ttt ggt gtc ttg gct acc cac tta cca aga gat tca aag tct atc       2272
Ser Phe Gly Val Leu Ala Thr His Leu Pro Arg Asp Ser Lys Ser Ile           
            400                 405                 410                   

tcc caa gtc tta tgg ggt tcc att ggt ttc tcc tta cca gct gca gtt       2320
Ser Gln Val Leu Trp Gly Ser Ile Gly Phe Ser Leu Pro Ala Ala Val           
        415                 420                 425                       

ggt gct gca ttt gct gct gaa gat gca cac aaa caa act ggc gaa caa       2368
Gly Ala Ala Phe Ala Ala Glu Asp Ala His Lys Gln Thr Gly Glu Gln           
    430                 435                 440                           

gaa aga aga act gtt ttg ttt att ggt gat ggt tct tta caa ttg act       2416
Glu Arg Arg Thr Val Leu Phe Ile Gly Asp Gly Ser Leu Gln Leu Thr           
445                 450                 455                 460           

gtc caa tca atc tca gat gct gca aga tgg aac atc aag cca tac atc       2464
Val Gln Ser Ile Ser Asp Ala Ala Arg Trp Asn Ile Lys Pro Tyr Ile           
                465                 470                 475               

ttc atc tta aac aac aga ggt tac act atc gaa aag ttg atc cac ggt       2512
Phe Ile Leu Asn Asn Arg Gly Tyr Thr Ile Glu Lys Leu Ile His Gly           
            480                 485                 490                   

cgt cat gag gac tac aac caa att caa cca tgg gat cac caa ttg tta       2560
Arg His Glu Asp Tyr Asn Gln Ile Gln Pro Trp Asp His Gln Leu Leu           
        495                 500                 505                       

ttg aag ctc ttt gct gac aag acc caa tat gaa aac cat gtt gtt aaa       2608
Leu Lys Leu Phe Ala Asp Lys Thr Gln Tyr Glu Asn His Val Val Lys           
    510                 515                 520                           

tcc gct aag gac ttg gac gct ttg atg aag gat gaa gca ttc aac aag       2656
Ser Ala Lys Asp Leu Asp Ala Leu Met Lys Asp Glu Ala Phe Asn Lys           
525                 530                 535                 540           

gaa gat aag att aga gtc att gaa tta ttc ttg gat gaa ttc gat gct       2704
Glu Asp Lys Ile Arg Val Ile Glu Leu Phe Leu Asp Glu Phe Asp Ala           
                545                 550                 555               

cca gaa atc ttg gtt gct caa gct aaa tta tct gat gaa atc aac tct       2752
Pro Glu Ile Leu Val Ala Gln Ala Lys Leu Ser Asp Glu Ile Asn Ser           
            560                 565                 570                   

aaa gcc gct taa tgacatctga atgtaaaatg aacattaaaa tgaattacta           2804
Lys Ala Ala                                                               
        575                                                               

aactttacgt ctactttaca atctataaac tttgtttaat catataacga aatacactaa     2864

tacacaatcc tgtacgtatg taatactttt atccatcaag gattgagaaa aaaaagtaat     2924

gattccctgg gccattaaaa cttagacccc cgagcttgga taggtcactc tctattttcg     2984

tttctccctt ccctgataga agggtgatat gtaattaaga ataatatata attttataat     3044

aaaaactaaa acaatccatc aatctcacca tcttcgttga cttcaacatt cataaatccg     3104

gcataagttg atagacctgg aattgtcatg atctttgcag ctagtgcata taaatatcct     3164

gctcctgcac ttattctaac ttctctgatt gggaagatga aatcctttgg aacacctttc     3224

aatgttggat catgggagag agaatattgc gtctttgcaa tacaaacagg cagcttgcca     3284

aaaccttgac tctcatactc ttcaatctgt ttcttggcca actcagatag ttcaatatct     3344

tttgctccat acatcttggt ggcaattgtt cttagtctat cttctaacga accatctaat     3404

gaataaagat agttaggagg ttgactttct tccaacggtc tttcctcggt agcacgtaca     3464

attgcctcag ctaacttgat tgcaccctta ccaccttgtg accaatggtc tgactcaaca     3524

gcatcaaatg caccagcctt gatggcttct tctctaatgg tctcaatttc cttctcggtg     3584

tcggtagcaa atttgttaat tgcaacaaca actggggccc cataactctt ggcattttca     3644

atttgttttc ttaagttgga tgatgcacca ttcctaacaa attcaatgtt ttcagtgacg     3704

tattctgttg gcaatacttg accaggtttg acgtcgcttg caccaccatg caacttc        3761


<210>  32
<211>  575
<212>  PRT
<213>  Issatchenkia orientalis

<400>  32

Met Thr Asp Lys Ile Ser Leu Gly Thr Tyr Leu Phe Glu Lys Leu Lys 
1               5                   10                  15      


Glu Ala Gly Ser Tyr Ser Ile Phe Gly Val Pro Gly Asp Phe Asn Leu 
            20                  25                  30          


Ala Leu Leu Asp His Val Lys Glu Val Glu Gly Ile Arg Trp Val Gly 
        35                  40                  45              


Asn Ala Asn Glu Leu Asn Ala Gly Tyr Glu Ala Asp Gly Tyr Ala Arg 
    50                  55                  60                  


Ile Asn Gly Phe Ala Ser Leu Ile Thr Thr Phe Gly Val Gly Glu Leu 
65                  70                  75                  80  


Ser Ala Val Asn Ala Ile Ala Gly Ser Tyr Ala Glu His Val Pro Leu 
                85                  90                  95      


Ile His Ile Val Gly Met Pro Ser Leu Ser Ala Met Lys Asn Asn Leu 
            100                 105                 110         


Leu Leu His His Thr Leu Gly Asp Thr Arg Phe Asp Asn Phe Thr Glu 
        115                 120                 125             


Met Ser Lys Lys Ile Ser Ala Lys Val Glu Ile Val Tyr Asp Leu Glu 
    130                 135                 140                 


Ser Ala Pro Lys Leu Ile Asn Asn Leu Ile Glu Thr Ala Tyr His Thr 
145                 150                 155                 160 


Lys Arg Pro Val Tyr Leu Gly Leu Pro Ser Asn Phe Ala Asp Glu Leu 
                165                 170                 175     


Val Pro Ala Ala Leu Val Lys Glu Asn Lys Leu His Leu Glu Glu Pro 
            180                 185                 190         


Leu Asn Asn Pro Val Ala Glu Glu Glu Phe Ile His Asn Val Val Glu 
        195                 200                 205             


Met Val Lys Lys Ala Glu Lys Pro Ile Ile Leu Val Asp Ala Cys Ala 
    210                 215                 220                 


Ala Arg His Asn Ile Ser Lys Glu Val Arg Glu Leu Ala Lys Leu Thr 
225                 230                 235                 240 


Lys Phe Pro Val Phe Thr Thr Pro Met Gly Lys Ser Thr Val Asp Glu 
                245                 250                 255     


Asp Asp Glu Glu Phe Phe Gly Leu Tyr Leu Gly Ser Leu Ser Ala Pro 
            260                 265                 270         


Asp Val Lys Asp Ile Val Gly Pro Thr Asp Cys Ile Leu Ser Leu Gly 
        275                 280                 285             


Gly Leu Pro Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Gly Tyr Thr 
    290                 295                 300                 


Thr Lys Asn Val Val Glu Phe His Ser Asn Tyr Cys Lys Phe Lys Ser 
305                 310                 315                 320 


Ala Thr Tyr Glu Asn Leu Met Met Lys Gly Ala Val Gln Arg Leu Ile 
                325                 330                 335     


Ser Glu Leu Lys Asn Ile Lys Tyr Ser Asn Val Ser Thr Leu Ser Pro 
            340                 345                 350         


Pro Lys Ser Lys Phe Ala Tyr Glu Ser Ala Lys Val Ala Pro Glu Gly 
        355                 360                 365             


Ile Ile Thr Gln Asp Tyr Leu Trp Lys Arg Leu Ser Tyr Phe Leu Lys 
    370                 375                 380                 


Pro Arg Asp Ile Ile Val Thr Glu Thr Gly Thr Ser Ser Phe Gly Val 
385                 390                 395                 400 


Leu Ala Thr His Leu Pro Arg Asp Ser Lys Ser Ile Ser Gln Val Leu 
                405                 410                 415     


Trp Gly Ser Ile Gly Phe Ser Leu Pro Ala Ala Val Gly Ala Ala Phe 
            420                 425                 430         


Ala Ala Glu Asp Ala His Lys Gln Thr Gly Glu Gln Glu Arg Arg Thr 
        435                 440                 445             


Val Leu Phe Ile Gly Asp Gly Ser Leu Gln Leu Thr Val Gln Ser Ile 
    450                 455                 460                 


Ser Asp Ala Ala Arg Trp Asn Ile Lys Pro Tyr Ile Phe Ile Leu Asn 
465                 470                 475                 480 


Asn Arg Gly Tyr Thr Ile Glu Lys Leu Ile His Gly Arg His Glu Asp 
                485                 490                 495     


Tyr Asn Gln Ile Gln Pro Trp Asp His Gln Leu Leu Leu Lys Leu Phe 
            500                 505                 510         


Ala Asp Lys Thr Gln Tyr Glu Asn His Val Val Lys Ser Ala Lys Asp 
        515                 520                 525             


Leu Asp Ala Leu Met Lys Asp Glu Ala Phe Asn Lys Glu Asp Lys Ile 
    530                 535                 540                 


Arg Val Ile Glu Leu Phe Leu Asp Glu Phe Asp Ala Pro Glu Ile Leu 
545                 550                 555                 560 


Val Ala Gln Ala Lys Leu Ser Asp Glu Ile Asn Ser Lys Ala Ala 
                565                 570                 575 


<210>  33
<211>  3179
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1052)..(2182)

<400>  33
gatttggacc tacaaggtgc tgtaaagagt atgaacactt ctggggagga ggaatggaac       60

agtgatgacg atgatgatga agaaagtgac gaaagtaacg aaagtgatta ctattcttac      120

gatgaaggcg aagaaacaga tgatagtgag ggagcccaag agggagagga agacgaaaat      180

gaacgaatca ttgaagctct aagtagtggt gttggtgaac tcaagatgga ctctttaggt      240

aattatattc ttgaatagtt gtgtaaagcg aatatgcaaa tagatttgtt ttataattat      300

gcatctcttt gaaagaggtt tagaggcaaa gttcttgcat acaatattgt gattgtttta      360

atgtcattct tgattttcat aaagagatta aaaaaaaaaa aaaaaaactt ataaaattga      420

gtagaaccat ttatatataa gacaaagatt gtctgtatta gtcctcaaca cactaaacct      480

tacatactta gggtaaattt gctaatagag tgatatgttc atgagaactc caacgacaac      540

acaaccacct atttgcacaa caaacaccat tgtcgcacgc tgcgcgccct agaagtagaa      600

agaaagggaa atgacattaa gagaatcata ccccgtgccc gtaacgccga aaaaatcaca      660

ccccgtcccc cacaccttaa aacctcaacc gcttaacacc gccacaccct ttctctttat      720

aaacgccgtt tgcattactc attcttctta taaaccgcac cccccaaaac gcggaatagc      780

ttcaaccccc caatcagata tgagtttccc gggaaacccg cttttcccga cagccccaca      840

aggggttggt ctataaaaga ggacgttttc cccgtcatcg agattgaaga ttcttacagg      900

cccatttatt caaattggag ttgattcttc ttgtctttac tttctttctc tctttttctt      960

ccttttttaa tattatcttt tgtcaagcct ggttccctaa gttgaactct cttttcttgt     1020

gatcctccta tatagatacg ccttgccaaa t atg ttt gca tca acc ttc aga        1072
                                   Met Phe Ala Ser Thr Phe Arg            
                                   1               5                      

agt caa gct gta aga gct gca aga ttt act aga ttc caa tcc act ttt       1120
Ser Gln Ala Val Arg Ala Ala Arg Phe Thr Arg Phe Gln Ser Thr Phe           
        10                  15                  20                        

gcc att cct gag aag caa atg ggt gtt atc ttt gaa act cat ggt ggt       1168
Ala Ile Pro Glu Lys Gln Met Gly Val Ile Phe Glu Thr His Gly Gly           
    25                  30                  35                            

cct tta caa tac aag gaa att cca gtt cca aaa cca aaa cca act gaa       1216
Pro Leu Gln Tyr Lys Glu Ile Pro Val Pro Lys Pro Lys Pro Thr Glu           
40                  45                  50                  55            

att tta atc aat gtt aaa tac tct ggt gtc tgc cat acc gat tta cac       1264
Ile Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His           
                60                  65                  70                

gca tgg aaa ggt gac tgg cca tta cca gca aag tta ccc cta gtt ggt       1312
Ala Trp Lys Gly Asp Trp Pro Leu Pro Ala Lys Leu Pro Leu Val Gly           
            75                  80                  85                    

ggt cac gaa ggt gcg ggc att gtt gtt gcg aaa ggt tct gca gtt acc       1360
Gly His Glu Gly Ala Gly Ile Val Val Ala Lys Gly Ser Ala Val Thr           
        90                  95                  100                       

aac ttt gag att ggc gat tat gct ggt att aag tgg tta aac ggt tca       1408
Asn Phe Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly Ser           
    105                 110                 115                           

tgt atg tca tgt gaa ttc tgt gaa caa ggt gat gaa tct aac tgt gaa       1456
Cys Met Ser Cys Glu Phe Cys Glu Gln Gly Asp Glu Ser Asn Cys Glu           
120                 125                 130                 135           

cat gcc gat ttg agt ggt tat act cat gat ggt tct ttc caa caa tat       1504
His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr           
                140                 145                 150               

gcc act gct gac gct att caa gct gca aag atc cca aag ggt acc gac       1552
Ala Thr Ala Asp Ala Ile Gln Ala Ala Lys Ile Pro Lys Gly Thr Asp           
            155                 160                 165                   

tta tct gaa gtt gcg cca att tta tgt gct ggt gtt act gtc tat aaa       1600
Leu Ser Glu Val Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys           
        170                 175                 180                       

gct ttg aaa act gct gat tta aga gca ggt caa tgg gtt gcg att tct       1648
Ala Leu Lys Thr Ala Asp Leu Arg Ala Gly Gln Trp Val Ala Ile Ser           
    185                 190                 195                           

ggt gcc gct ggt ggt cta ggt tct ctt gct gtc caa tat gca aag gca       1696
Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala           
200                 205                 210                 215           

atg ggt cta aga gtt tta ggt atc gat ggt ggt gaa ggt aaa aag gaa       1744
Met Gly Leu Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Lys Glu           
                220                 225                 230               

ctt ttt gaa caa tgt ggt ggt gat gtg ttt atc gat ttc acc aga tac       1792
Leu Phe Glu Gln Cys Gly Gly Asp Val Phe Ile Asp Phe Thr Arg Tyr           
            235                 240                 245                   

cca aga gat gca cct gaa aag atg gtt gct gat att aag gct gca act       1840
Pro Arg Asp Ala Pro Glu Lys Met Val Ala Asp Ile Lys Ala Ala Thr           
        250                 255                 260                       

aac ggt ttg ggt cca cac ggt gtt atc aat gtc tct gtc tcc cca gct       1888
Asn Gly Leu Gly Pro His Gly Val Ile Asn Val Ser Val Ser Pro Ala           
    265                 270                 275                           

gct atc tct caa tca tgt gac tat gtt aga gca act ggt aag gtt gtc       1936
Ala Ile Ser Gln Ser Cys Asp Tyr Val Arg Ala Thr Gly Lys Val Val           
280                 285                 290                 295           

ctt gtc ggt atg cca tct ggt gct gtc tgt aag tct gat gtc ttc act       1984
Leu Val Gly Met Pro Ser Gly Ala Val Cys Lys Ser Asp Val Phe Thr           
                300                 305                 310               

cat gtt gtt aaa tcc tta caa att aaa ggt tct tat gtt ggt aac aga       2032
His Val Val Lys Ser Leu Gln Ile Lys Gly Ser Tyr Val Gly Asn Arg           
            315                 320                 325                   

gca gat acc aga gaa gct ttg gaa ttc ttt aat gaa ggt aag gtc aga       2080
Ala Asp Thr Arg Glu Ala Leu Glu Phe Phe Asn Glu Gly Lys Val Arg           
        330                 335                 340                       

tct cca atc aag gtt gtc cca tta tct act tta cct gaa att tac gaa       2128
Ser Pro Ile Lys Val Val Pro Leu Ser Thr Leu Pro Glu Ile Tyr Glu           
    345                 350                 355                           

ttg atg gag caa ggt aag att tta ggt aga tac gtt gtt gat act tct       2176
Leu Met Glu Gln Gly Lys Ile Leu Gly Arg Tyr Val Val Asp Thr Ser           
360                 365                 370                 375           

aaa taa tgaagatgaa gaaaacagca aactttttat gactaccccc aaccatctaa        2232
Lys                                                                       
                                                                          

cgatttatga tctatatata gctttctaga acatccattt atttattcac ttactcatgt     2292

atttatatta tataatacaa aataactaat tacaatgtgt acattttttt ttttcattac     2352

cataatgtat gcgttgagcc tcttgcacct tctttattag gaaatcagtt gaaaaatttc     2412

cggattgtct ttattattgg cccatttttt tttggtcaca cctttatttt tgtacacttc     2472

tcgggcaaag caaaaactat agtaccggat aggcctttat aaaactccag tgtgtatgat     2532

tttagttggt gtgccatcta cacgttctct tagtttcttt atcatgtcac agaaagcaag     2592

catgcaaacc cttacaaaaa ataacaacat acaaatgcct aaacaactgg actataatga     2652

tggtgagtca gttacgaaaa gagcaagtgg gttaatacga tttcgtaagg gacagtctga     2712

ggaagactac aattttcaaa aggagcagtt ctggtccacg ggtcctttag tacagaatca     2772

cacatttgtg actgaatttg ttgaaaagtt tattgaaaac acaattagtg aagattattc     2832

aatcacagat agatcgaaaa tagaacgtga aacaatcata cacggattgg agaagctgta     2892

ttttcaaagg gaatatgagc gatgtctaaa agatgttcaa ctattgaagg acaatatcga     2952

taagttcaat cctaatttgg atcttaatga aaagaattta taatgagctg aattatattt     3012

cttggatgtg catcaaaaag atccatgaga gtaacgaaaa gaaactgggg gaaatctaat     3072

aatttacaat ttcaatatac acttctatat cctttaatgt aatggcttta taaataaaca     3132

cgaacttcta cagcaccgac gtttcttttt cttaccagct cctcttc                   3179


<210>  34
<211>  376
<212>  PRT
<213>  Issatchenkia orientalis

<400>  34

Met Phe Ala Ser Thr Phe Arg Ser Gln Ala Val Arg Ala Ala Arg Phe 
1               5                   10                  15      


Thr Arg Phe Gln Ser Thr Phe Ala Ile Pro Glu Lys Gln Met Gly Val 
            20                  25                  30          


Ile Phe Glu Thr His Gly Gly Pro Leu Gln Tyr Lys Glu Ile Pro Val 
        35                  40                  45              


Pro Lys Pro Lys Pro Thr Glu Ile Leu Ile Asn Val Lys Tyr Ser Gly 
    50                  55                  60                  


Val Cys His Thr Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Pro 
65                  70                  75                  80  


Ala Lys Leu Pro Leu Val Gly Gly His Glu Gly Ala Gly Ile Val Val 
                85                  90                  95      


Ala Lys Gly Ser Ala Val Thr Asn Phe Glu Ile Gly Asp Tyr Ala Gly 
            100                 105                 110         


Ile Lys Trp Leu Asn Gly Ser Cys Met Ser Cys Glu Phe Cys Glu Gln 
        115                 120                 125             


Gly Asp Glu Ser Asn Cys Glu His Ala Asp Leu Ser Gly Tyr Thr His 
    130                 135                 140                 


Asp Gly Ser Phe Gln Gln Tyr Ala Thr Ala Asp Ala Ile Gln Ala Ala 
145                 150                 155                 160 


Lys Ile Pro Lys Gly Thr Asp Leu Ser Glu Val Ala Pro Ile Leu Cys 
                165                 170                 175     


Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ala 
            180                 185                 190         


Gly Gln Trp Val Ala Ile Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu 
        195                 200                 205             


Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Arg Val Leu Gly Ile Asp 
    210                 215                 220                 


Gly Gly Glu Gly Lys Lys Glu Leu Phe Glu Gln Cys Gly Gly Asp Val 
225                 230                 235                 240 


Phe Ile Asp Phe Thr Arg Tyr Pro Arg Asp Ala Pro Glu Lys Met Val 
                245                 250                 255     


Ala Asp Ile Lys Ala Ala Thr Asn Gly Leu Gly Pro His Gly Val Ile 
            260                 265                 270         


Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Cys Asp Tyr Val 
        275                 280                 285             


Arg Ala Thr Gly Lys Val Val Leu Val Gly Met Pro Ser Gly Ala Val 
    290                 295                 300                 


Cys Lys Ser Asp Val Phe Thr His Val Val Lys Ser Leu Gln Ile Lys 
305                 310                 315                 320 


Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Glu Phe 
                325                 330                 335     


Phe Asn Glu Gly Lys Val Arg Ser Pro Ile Lys Val Val Pro Leu Ser 
            340                 345                 350         


Thr Leu Pro Glu Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu Gly 
        355                 360                 365             


Arg Tyr Val Val Asp Thr Ser Lys 
    370                 375     


<210>  35
<211>  3181
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1015)..(2181)

<400>  35
tttttctttt ttttttcttt ttttttcctc tttttctttt gttttgtttc gtttcttttc       60

cgccagttcc cgttttccat ttccggaaca acaatgggac tccactgttt tcttcccccc      120

ttcccttttc ggctcgcagt ctgtacatgc acgtttatcc gacacctgtc ttgtttggcg      180

cgtaattaat acagtttctc cggagtccag gtctcggacg ggtaatttac acgtcatcat      240

tcatttctgt gtcaagagag gtagcgcaaa aagtagaaat ggtgaaccac gggaatgact      300

tgctggaaat cgacgccaga gtccatttga aaacctacct ctacaagaga ggaaacacac      360

tacagggtgt ccctggtccg taaaatggcg taatatgatg acttccctct atagacgttg      420

tatttccagc tccaacatgg ttaaactatt gctatggtga tggtattaca gatagtaaaa      480

gaaggaaggg ggggtggcaa tctcacccta acagttacta agaacgtcta cttcatctac      540

tgtcaatata cattggccac atgccgagaa attacgtcga cgccaaagaa gggcccagcc      600

gaaaaaagaa atggaaaact tggccgaaaa gggaaacaaa caaaaaggtg atgtaaaatt      660

agcggaaagg ggaattggca aattgaggga gaaaaaaaaa aaggcagaaa aggaggcgga      720

aagtcagtac gttttgaagg cgtcattggt tttccctttt gcagagtgtt tcatttcttt      780

tgttttatga cgtagtggcg tttcttttcc tgcactttag aaatctatct tttccttatc      840

aagtaacaag cggttggcaa aggtgtatat aaatcaagga attcccactt tgaacccttt      900

gaattttgat atcgtttatt ttaaatttat tttatgtttc taatctcaaa gagtttacac      960

tttacaagga gtttctcaaa tactatcaag acattgaata gatcaaacat taaa atg       1017
                                                            Met           
                                                            1             

gtg tcc cct gct gaa aga tta tct act att gcg tcc aca atc aag cca       1065
Val Ser Pro Ala Glu Arg Leu Ser Thr Ile Ala Ser Thr Ile Lys Pro           
            5                   10                  15                    

aac aga aaa gat tct aca tca tta caa cca gaa gac tat ccg gaa cat       1113
Asn Arg Lys Asp Ser Thr Ser Leu Gln Pro Glu Asp Tyr Pro Glu His           
        20                  25                  30                        

ccg ttc aag gtg acg gtt gtt ggt tcc ggt aac tgg ggg tgt aca att       1161
Pro Phe Lys Val Thr Val Val Gly Ser Gly Asn Trp Gly Cys Thr Ile           
    35                  40                  45                            

gcc aag gtt ata gcg gaa aac acc gtt gag aga cct cgt caa ttt caa       1209
Ala Lys Val Ile Ala Glu Asn Thr Val Glu Arg Pro Arg Gln Phe Gln           
50                  55                  60                  65            

aga gat gtt aat atg tgg gtc tat gaa gaa ttg att gaa ggc gaa aag       1257
Arg Asp Val Asn Met Trp Val Tyr Glu Glu Leu Ile Glu Gly Glu Lys           
                70                  75                  80                

ttg act gaa atc ata aat acc aaa cac gaa aac gtc aag tac ttg cca       1305
Leu Thr Glu Ile Ile Asn Thr Lys His Glu Asn Val Lys Tyr Leu Pro           
            85                  90                  95                    

ggt atc aag ttg cca gtt aac gtt gtt gca gtt cca gac att gtt gag       1353
Gly Ile Lys Leu Pro Val Asn Val Val Ala Val Pro Asp Ile Val Glu           
        100                 105                 110                       

gct tgt gca ggc tca gac ttg att gtc ttt aat att cct cac caa ttt       1401
Ala Cys Ala Gly Ser Asp Leu Ile Val Phe Asn Ile Pro His Gln Phe           
    115                 120                 125                           

tta cca aga att tta tcc caa tta aag ggt aag gtg aat cca aag gct       1449
Leu Pro Arg Ile Leu Ser Gln Leu Lys Gly Lys Val Asn Pro Lys Ala           
130                 135                 140                 145           

aga gca att tct tgt ttg aaa ggt ttg gat gtc aat cct aat gga tgt       1497
Arg Ala Ile Ser Cys Leu Lys Gly Leu Asp Val Asn Pro Asn Gly Cys           
                150                 155                 160               

aag ttg ctc tcc act gtt att act gaa gag ttg ggt att tat tgt ggt       1545
Lys Leu Leu Ser Thr Val Ile Thr Glu Glu Leu Gly Ile Tyr Cys Gly           
            165                 170                 175                   

gcc tta tca ggt gct aat tta gct cct gaa gtt gca caa tgt aaa tgg       1593
Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Gln Cys Lys Trp           
        180                 185                 190                       

tcg gaa aca act gtt gca tat aca att ccg gac gat ttc aga ggt aaa       1641
Ser Glu Thr Thr Val Ala Tyr Thr Ile Pro Asp Asp Phe Arg Gly Lys           
    195                 200                 205                           

ggc aag gat att gac cat caa att cta aag agt ttg ttc cat aga cct       1689
Gly Lys Asp Ile Asp His Gln Ile Leu Lys Ser Leu Phe His Arg Pro           
210                 215                 220                 225           

tat ttc cat gtt cgt gtt att agt gat gtt gca ggt att tcc att gcc       1737
Tyr Phe His Val Arg Val Ile Ser Asp Val Ala Gly Ile Ser Ile Ala           
                230                 235                 240               

ggt gca ctc aag aat gtc gtt gct atg gct gct gga ttt gtc gaa ggt       1785
Gly Ala Leu Lys Asn Val Val Ala Met Ala Ala Gly Phe Val Glu Gly           
            245                 250                 255                   

tta ggt tgg ggt gat aat gca aag gct gca gtc atg aga ata ggt ttg       1833
Leu Gly Trp Gly Asp Asn Ala Lys Ala Ala Val Met Arg Ile Gly Leu           
        260                 265                 270                       

gtg gaa acc att caa ttt gcc aag act ttt ttc gat ggc tgt cat gct       1881
Val Glu Thr Ile Gln Phe Ala Lys Thr Phe Phe Asp Gly Cys His Ala           
    275                 280                 285                           

gca acc ttt act cat gaa tct gca ggt gtt gcc gac cta atc act acc       1929
Ala Thr Phe Thr His Glu Ser Ala Gly Val Ala Asp Leu Ile Thr Thr           
290                 295                 300                 305           

tgt gcc ggc ggc cgt aac gtt aga gtt ggt aga tat atg gca caa cat       1977
Cys Ala Gly Gly Arg Asn Val Arg Val Gly Arg Tyr Met Ala Gln His           
                310                 315                 320               

tct gtc tct gca acg gag gct gaa gaa aag ttg ttg aat ggc caa tcc       2025
Ser Val Ser Ala Thr Glu Ala Glu Glu Lys Leu Leu Asn Gly Gln Ser           
            325                 330                 335                   

tgt caa ggt atc cac aca act agg gaa gtt tac gag ttc ctc tcc aac       2073
Cys Gln Gly Ile His Thr Thr Arg Glu Val Tyr Glu Phe Leu Ser Asn           
        340                 345                 350                       

atg ggc agg aca gat gag ttc cca cta ttt acc acc acc tac cgt atc       2121
Met Gly Arg Thr Asp Glu Phe Pro Leu Phe Thr Thr Thr Tyr Arg Ile           
    355                 360                 365                           

atc tac gaa aac ttc cca att gag aag ctg cca gaa tgc ctt gaa cct       2169
Ile Tyr Glu Asn Phe Pro Ile Glu Lys Leu Pro Glu Cys Leu Glu Pro           
370                 375                 380                 385           

gtg gaa gat taa ataacctcag ggagaacttt ggcattgtac tctccattga           2221
Val Glu Asp                                                               
                                                                          

cgagtccgct aacccattct tgttaaacct aaccttgcat tatcacattc cctttgaccc     2281

cctttagctg catttccact tgtctacatt aagattcatt acacattctt tttcgtattt     2341

ctcttacctc cctcccccct ccatggatct tatatataaa tcttttctat aacaataata     2401

tctactagag ttaaacaaca attccacttg gcatggctgt ctcagcaaat ctgcttctac     2461

ctactgcacg ggtttgcatg tcattgtttc tagcagggaa tcgtccatgt acgttgtcct     2521

ccatgatggt cttcccgctg ccactttctt tagtatctta aatagagcag atcttacgtc     2581

cactgtgcat ccgtgcaccc cgaaaatcgt atggttttcc ttgccacctc tcacaatttt     2641

gaatatgctc aacgcgaaag agaggggaag aggaatcgca ttcgtagagt ggctacattc     2701

aaccctgaca aaggaatagc gtttgtgcag gagagagtgg tttgcataga tttcctttcc     2761

tttgcaagca tattatatag agtagccaat acagtaacag ctacagcaca aaaaagagaa     2821

cgagaacgag aacgagaaca agaacaagaa ctagcactac tgtcactgcc agcatcaaca     2881

tcactaccat tattccaaca tgtttgcaac tagaaatata accattggtg tcagaacact     2941

cagaccaacc agtttcttga aaacaaggtc ttttctgcaa cagaggctac aatcaacgct     3001

aaagaagagc tatgaaccaa ccaaatccga gctcgacatt gcaagcaaga tgtcctggga     3061

acagtttcta accatgagaa agcaacagag aagattggga ggcattgggt ccattgcagc     3121

agcaattttg gcaatgggcg cctccttctc gtatttctca cagattgaaa tcgatcctac     3181


<210>  36
<211>  388
<212>  PRT
<213>  Issatchenkia orientalis

<400>  36

Met Val Ser Pro Ala Glu Arg Leu Ser Thr Ile Ala Ser Thr Ile Lys 
1               5                   10                  15      


Pro Asn Arg Lys Asp Ser Thr Ser Leu Gln Pro Glu Asp Tyr Pro Glu 
            20                  25                  30          


His Pro Phe Lys Val Thr Val Val Gly Ser Gly Asn Trp Gly Cys Thr 
        35                  40                  45              


Ile Ala Lys Val Ile Ala Glu Asn Thr Val Glu Arg Pro Arg Gln Phe 
    50                  55                  60                  


Gln Arg Asp Val Asn Met Trp Val Tyr Glu Glu Leu Ile Glu Gly Glu 
65                  70                  75                  80  


Lys Leu Thr Glu Ile Ile Asn Thr Lys His Glu Asn Val Lys Tyr Leu 
                85                  90                  95      


Pro Gly Ile Lys Leu Pro Val Asn Val Val Ala Val Pro Asp Ile Val 
            100                 105                 110         


Glu Ala Cys Ala Gly Ser Asp Leu Ile Val Phe Asn Ile Pro His Gln 
        115                 120                 125             


Phe Leu Pro Arg Ile Leu Ser Gln Leu Lys Gly Lys Val Asn Pro Lys 
    130                 135                 140                 


Ala Arg Ala Ile Ser Cys Leu Lys Gly Leu Asp Val Asn Pro Asn Gly 
145                 150                 155                 160 


Cys Lys Leu Leu Ser Thr Val Ile Thr Glu Glu Leu Gly Ile Tyr Cys 
                165                 170                 175     


Gly Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Gln Cys Lys 
            180                 185                 190         


Trp Ser Glu Thr Thr Val Ala Tyr Thr Ile Pro Asp Asp Phe Arg Gly 
        195                 200                 205             


Lys Gly Lys Asp Ile Asp His Gln Ile Leu Lys Ser Leu Phe His Arg 
    210                 215                 220                 


Pro Tyr Phe His Val Arg Val Ile Ser Asp Val Ala Gly Ile Ser Ile 
225                 230                 235                 240 


Ala Gly Ala Leu Lys Asn Val Val Ala Met Ala Ala Gly Phe Val Glu 
                245                 250                 255     


Gly Leu Gly Trp Gly Asp Asn Ala Lys Ala Ala Val Met Arg Ile Gly 
            260                 265                 270         


Leu Val Glu Thr Ile Gln Phe Ala Lys Thr Phe Phe Asp Gly Cys His 
        275                 280                 285             


Ala Ala Thr Phe Thr His Glu Ser Ala Gly Val Ala Asp Leu Ile Thr 
    290                 295                 300                 


Thr Cys Ala Gly Gly Arg Asn Val Arg Val Gly Arg Tyr Met Ala Gln 
305                 310                 315                 320 


His Ser Val Ser Ala Thr Glu Ala Glu Glu Lys Leu Leu Asn Gly Gln 
                325                 330                 335     


Ser Cys Gln Gly Ile His Thr Thr Arg Glu Val Tyr Glu Phe Leu Ser 
            340                 345                 350         


Asn Met Gly Arg Thr Asp Glu Phe Pro Leu Phe Thr Thr Thr Tyr Arg 
        355                 360                 365             


Ile Ile Tyr Glu Asn Phe Pro Ile Glu Lys Leu Pro Glu Cys Leu Glu 
    370                 375                 380                 


Pro Val Glu Asp 
385             


<210>  37
<211>  2587
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (433)..(2115)

<400>  37
ccagttcgga aactaagaca taacaaaaaa gcatgggatg aatggggaaa aaatttagaa       60

atgtaaaaaa aaataggaaa ggggcttttc ctattgtcaa taaaacaact ctgttaggag      120

aaaatagttt tataatattt tcatggactc tttcccccac atttattacc gaattgggaa      180

attcccgggc aatatccctc gctccttaga aattccgtac tcccggggag ggctaactct      240

ccgagttatt cctaacactc tggggggaac aacattactc atagaatact ataaataccg      300

agcgtaccgc atatatgtag tatgtgacag aaattggtat ccgataatcc cttaccttgg      360

caaggttttg tttcaataaa acattcaaat aacttaaatt caaataatat tcatttaaat      420

tgctttaaaa aa atg gct cca act gct gtt gat atc cat aac gag tac aaa      471
              Met Ala Pro Thr Ala Val Asp Ile His Asn Glu Tyr Lys         
              1               5                   10                      

cag aat gtt tcc aac gaa cag gaa att cct ttc aac aaa act gaa aga        519
Gln Asn Val Ser Asn Glu Gln Glu Ile Pro Phe Asn Lys Thr Glu Arg           
    15                  20                  25                            

aag tca tcg att gca tct aaa tta gga ctg aat cca gac gct aag att        567
Lys Ser Ser Ile Ala Ser Lys Leu Gly Leu Asn Pro Asp Ala Lys Ile           
30                  35                  40                  45            

cac tac aat tct gct gtt cct ata tta tac gaa gat ggt tta aag gaa        615
His Tyr Asn Ser Ala Val Pro Ile Leu Tyr Glu Asp Gly Leu Lys Glu           
                50                  55                  60                

aaa ggt aca acc att tcc tct tct ggt gca ttg att gca ttc tct ggt        663
Lys Gly Thr Thr Ile Ser Ser Ser Gly Ala Leu Ile Ala Phe Ser Gly           
            65                  70                  75                    

tcc aaa aca ggt aga tct cca aag gac aaa aga att gtc gat gaa gag        711
Ser Lys Thr Gly Arg Ser Pro Lys Asp Lys Arg Ile Val Asp Glu Glu           
        80                  85                  90                        

act tca aca gac aac atc tgg tgg ggt cca gtc aat aag aag gtt gat        759
Thr Ser Thr Asp Asn Ile Trp Trp Gly Pro Val Asn Lys Lys Val Asp           
    95                  100                 105                           

gaa aac act tgg aat atc tcg aaa tct aga gcg att gat tat ttg aga        807
Glu Asn Thr Trp Asn Ile Ser Lys Ser Arg Ala Ile Asp Tyr Leu Arg           
110                 115                 120                 125           

aca aga gag aag gtt tac att atc gat gct ttt gct ggt tgg gat cca        855
Thr Arg Glu Lys Val Tyr Ile Ile Asp Ala Phe Ala Gly Trp Asp Pro           
                130                 135                 140               

aga tac aga att aag gtt aga att gtc tgt gct aga gct tac cat gct        903
Arg Tyr Arg Ile Lys Val Arg Ile Val Cys Ala Arg Ala Tyr His Ala           
            145                 150                 155                   

ttg ttc atg aag aat atg tta att aga cca aca acg gaa gaa tta aag        951
Leu Phe Met Lys Asn Met Leu Ile Arg Pro Thr Thr Glu Glu Leu Lys           
        160                 165                 170                       

aac ttt ggt gag cct gat ttc acc att tgg aat gca ggt caa ttc cct        999
Asn Phe Gly Glu Pro Asp Phe Thr Ile Trp Asn Ala Gly Gln Phe Pro           
    175                 180                 185                           

gct aat gtt tac act aag ggt atg act tct tca act tct gtt gaa ata       1047
Ala Asn Val Tyr Thr Lys Gly Met Thr Ser Ser Thr Ser Val Glu Ile           
190                 195                 200                 205           

aat ttc aag tct atg gaa atg gtt atc cta ggt act gaa tac gca ggt       1095
Asn Phe Lys Ser Met Glu Met Val Ile Leu Gly Thr Glu Tyr Ala Gly           
                210                 215                 220               

gaa atg aag aaa ggt atc ttt acc gtt atg ttc tac ttg atg cca atc       1143
Glu Met Lys Lys Gly Ile Phe Thr Val Met Phe Tyr Leu Met Pro Ile           
            225                 230                 235                   

aga cac aag gtt tta act tta cac tct tct gca aat caa ggt aaa aag       1191
Arg His Lys Val Leu Thr Leu His Ser Ser Ala Asn Gln Gly Lys Lys           
        240                 245                 250                       

gat ggt gat gtc aca tta ttc ttt ggt tta tct ggt aca ggt aaa aca       1239
Asp Gly Asp Val Thr Leu Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr           
    255                 260                 265                           

acc ttg tct gca gat cct cat aga gaa ttg att ggt gat gat gaa cat       1287
Thr Leu Ser Ala Asp Pro His Arg Glu Leu Ile Gly Asp Asp Glu His           
270                 275                 280                 285           

tgc tgg tct gat cat ggt gtt ttc aac att gaa ggt gga tgt tat gct       1335
Cys Trp Ser Asp His Gly Val Phe Asn Ile Glu Gly Gly Cys Tyr Ala           
                290                 295                 300               

aag tgt ttg gac tta tct gct gaa aga gaa cct gag att ttc aat gca       1383
Lys Cys Leu Asp Leu Ser Ala Glu Arg Glu Pro Glu Ile Phe Asn Ala           
            305                 310                 315                   

att agg ttt gga tct gtc ttg gag aat gtt gtc tat gat cca gtt gat       1431
Ile Arg Phe Gly Ser Val Leu Glu Asn Val Val Tyr Asp Pro Val Asp           
        320                 325                 330                       

aga act gtt gac tat tcc gct gct aat gtc act gaa aat act aga tgt       1479
Arg Thr Val Asp Tyr Ser Ala Ala Asn Val Thr Glu Asn Thr Arg Cys           
    335                 340                 345                           

gct tat cct atc gac ttt att cct tct gct aag atc cca tgt ctg gca       1527
Ala Tyr Pro Ile Asp Phe Ile Pro Ser Ala Lys Ile Pro Cys Leu Ala           
350                 355                 360                 365           

gat tct cat cca aag aat att gtt ctt tta act tgt gat gca aga ggt       1575
Asp Ser His Pro Lys Asn Ile Val Leu Leu Thr Cys Asp Ala Arg Gly           
                370                 375                 380               

gtt ttg cca cct gtc tcc aag cta act aat gca caa gtc atg tat cac       1623
Val Leu Pro Pro Val Ser Lys Leu Thr Asn Ala Gln Val Met Tyr His           
            385                 390                 395                   

ttt atc tct ggt tac acc tcc aag atg gca ggt acc gaa gtt ggt gtc       1671
Phe Ile Ser Gly Tyr Thr Ser Lys Met Ala Gly Thr Glu Val Gly Val           
        400                 405                 410                       

act gaa cca gaa gca acc ttc tct gca tgt ttt ggt caa cct ttc tta       1719
Thr Glu Pro Glu Ala Thr Phe Ser Ala Cys Phe Gly Gln Pro Phe Leu           
    415                 420                 425                           

gtt tta cat cca atg aaa tac gca caa caa ctc tct gat aaa atg gct       1767
Val Leu His Pro Met Lys Tyr Ala Gln Gln Leu Ser Asp Lys Met Ala           
430                 435                 440                 445           

gaa cat tct tcc acc gct tgg tta ttg aat acc ggt tgg act ggt caa       1815
Glu His Ser Ser Thr Ala Trp Leu Leu Asn Thr Gly Trp Thr Gly Gln           
                450                 455                 460               

tct tat gtt aaa ggt ggt aag aga tgt cca ttg aag tat act aga gca       1863
Ser Tyr Val Lys Gly Gly Lys Arg Cys Pro Leu Lys Tyr Thr Arg Ala           
            465                 470                 475                   

att tta gat gct att cac tct ggt gag ctt gca aaa cag gaa ttc gaa       1911
Ile Leu Asp Ala Ile His Ser Gly Glu Leu Ala Lys Gln Glu Phe Glu           
        480                 485                 490                       

aca tac cct act ttc ggt tta caa gtt cca aaa act tgt cca ggt gtc       1959
Thr Tyr Pro Thr Phe Gly Leu Gln Val Pro Lys Thr Cys Pro Gly Val           
    495                 500                 505                           

cca gaa agt gtt ctg aac cca tct aaa cac tgg gct act ggt gaa gct       2007
Pro Glu Ser Val Leu Asn Pro Ser Lys His Trp Ala Thr Gly Glu Ala           
510                 515                 520                 525           

gat ttc aag gct gaa gtc act aac ttg gct aaa tta ttt gct gag aac       2055
Asp Phe Lys Ala Glu Val Thr Asn Leu Ala Lys Leu Phe Ala Glu Asn           
                530                 535                 540               

ttt gaa aag tat tct gca gaa tgt act gca gaa gtt gtt gct gct ggt       2103
Phe Glu Lys Tyr Ser Ala Glu Cys Thr Ala Glu Val Val Ala Ala Gly           
            545                 550                 555                   

cct gct tta taa atggtttgat atcaatatta tgactcggtt atgtttgtgt           2155
Pro Ala Leu                                                               
        560                                                               

atttaattcc ctctttttgt ttattctttt tttcttccac ccttgtttat cattagtgtc     2215

gcccttttct tttcaaagga caattattag catttatata aaattcaatt cagtactata     2275

ttcgatcctt ttaatcactt cagtgtgtcg tgtaggatta ataccagagc tagaaacaat     2335

atggattgga catgtctacc aattatatta tatgtatata tgaatctaat tatctctata     2395

taaaatcaat taactaaatg tttattgatc ggtaatttcc catcaattaa tactggttta     2455

ggaatatatt tgtcatcaac ttgaatctcg aaacctggtt tacaggatag tttttcatga     2515

aattggataa tcagtttcga tagtggttcc gcgtaccatc tctcattgtt ccatttgaaa     2575

aagccaacat gg                                                         2587


<210>  38
<211>  560
<212>  PRT
<213>  Issatchenkia orientalis

<400>  38

Met Ala Pro Thr Ala Val Asp Ile His Asn Glu Tyr Lys Gln Asn Val 
1               5                   10                  15      


Ser Asn Glu Gln Glu Ile Pro Phe Asn Lys Thr Glu Arg Lys Ser Ser 
            20                  25                  30          


Ile Ala Ser Lys Leu Gly Leu Asn Pro Asp Ala Lys Ile His Tyr Asn 
        35                  40                  45              


Ser Ala Val Pro Ile Leu Tyr Glu Asp Gly Leu Lys Glu Lys Gly Thr 
    50                  55                  60                  


Thr Ile Ser Ser Ser Gly Ala Leu Ile Ala Phe Ser Gly Ser Lys Thr 
65                  70                  75                  80  


Gly Arg Ser Pro Lys Asp Lys Arg Ile Val Asp Glu Glu Thr Ser Thr 
                85                  90                  95      


Asp Asn Ile Trp Trp Gly Pro Val Asn Lys Lys Val Asp Glu Asn Thr 
            100                 105                 110         


Trp Asn Ile Ser Lys Ser Arg Ala Ile Asp Tyr Leu Arg Thr Arg Glu 
        115                 120                 125             


Lys Val Tyr Ile Ile Asp Ala Phe Ala Gly Trp Asp Pro Arg Tyr Arg 
    130                 135                 140                 


Ile Lys Val Arg Ile Val Cys Ala Arg Ala Tyr His Ala Leu Phe Met 
145                 150                 155                 160 


Lys Asn Met Leu Ile Arg Pro Thr Thr Glu Glu Leu Lys Asn Phe Gly 
                165                 170                 175     


Glu Pro Asp Phe Thr Ile Trp Asn Ala Gly Gln Phe Pro Ala Asn Val 
            180                 185                 190         


Tyr Thr Lys Gly Met Thr Ser Ser Thr Ser Val Glu Ile Asn Phe Lys 
        195                 200                 205             


Ser Met Glu Met Val Ile Leu Gly Thr Glu Tyr Ala Gly Glu Met Lys 
    210                 215                 220                 


Lys Gly Ile Phe Thr Val Met Phe Tyr Leu Met Pro Ile Arg His Lys 
225                 230                 235                 240 


Val Leu Thr Leu His Ser Ser Ala Asn Gln Gly Lys Lys Asp Gly Asp 
                245                 250                 255     


Val Thr Leu Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu Ser 
            260                 265                 270         


Ala Asp Pro His Arg Glu Leu Ile Gly Asp Asp Glu His Cys Trp Ser 
        275                 280                 285             


Asp His Gly Val Phe Asn Ile Glu Gly Gly Cys Tyr Ala Lys Cys Leu 
    290                 295                 300                 


Asp Leu Ser Ala Glu Arg Glu Pro Glu Ile Phe Asn Ala Ile Arg Phe 
305                 310                 315                 320 


Gly Ser Val Leu Glu Asn Val Val Tyr Asp Pro Val Asp Arg Thr Val 
                325                 330                 335     


Asp Tyr Ser Ala Ala Asn Val Thr Glu Asn Thr Arg Cys Ala Tyr Pro 
            340                 345                 350         


Ile Asp Phe Ile Pro Ser Ala Lys Ile Pro Cys Leu Ala Asp Ser His 
        355                 360                 365             


Pro Lys Asn Ile Val Leu Leu Thr Cys Asp Ala Arg Gly Val Leu Pro 
    370                 375                 380                 


Pro Val Ser Lys Leu Thr Asn Ala Gln Val Met Tyr His Phe Ile Ser 
385                 390                 395                 400 


Gly Tyr Thr Ser Lys Met Ala Gly Thr Glu Val Gly Val Thr Glu Pro 
                405                 410                 415     


Glu Ala Thr Phe Ser Ala Cys Phe Gly Gln Pro Phe Leu Val Leu His 
            420                 425                 430         


Pro Met Lys Tyr Ala Gln Gln Leu Ser Asp Lys Met Ala Glu His Ser 
        435                 440                 445             


Ser Thr Ala Trp Leu Leu Asn Thr Gly Trp Thr Gly Gln Ser Tyr Val 
    450                 455                 460                 


Lys Gly Gly Lys Arg Cys Pro Leu Lys Tyr Thr Arg Ala Ile Leu Asp 
465                 470                 475                 480 


Ala Ile His Ser Gly Glu Leu Ala Lys Gln Glu Phe Glu Thr Tyr Pro 
                485                 490                 495     


Thr Phe Gly Leu Gln Val Pro Lys Thr Cys Pro Gly Val Pro Glu Ser 
            500                 505                 510         


Val Leu Asn Pro Ser Lys His Trp Ala Thr Gly Glu Ala Asp Phe Lys 
        515                 520                 525             


Ala Glu Val Thr Asn Leu Ala Lys Leu Phe Ala Glu Asn Phe Glu Lys 
    530                 535                 540                 


Tyr Ser Ala Glu Cys Thr Ala Glu Val Val Ala Ala Gly Pro Ala Leu 
545                 550                 555                 560 


<210>  39
<211>  2562
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (363)..(2174)

<400>  39
ctactatgta cactgtataa gtaaaaagac gatacccccc tcccactctg ggtgctacgg       60

tgtagatctc tccgtaaaca caaaaaggcg gctcagatga taattggggt ccgggcgcaa      120

ccggaagggg ggagagaggg gagcgatggc ttctcctccg gggggctacg ggagtttcct      180

ctttgggaag gataaagagg ggatggattg atacaagatt ctgagaacct attacgatga      240

tgttcagtgg tattttgtct tttgttattt aaagggaggg gactttcctc aataccttag      300

ttgtaaaatt acgctattat ctttaaccct ttcttttgag caataattaa aaagaacacg      360

cc atg aca aaa gat tgc tgt gag gtt aca aag att cca gtg gga gag         407
   Met Thr Lys Asp Cys Cys Glu Val Thr Lys Ile Pro Val Gly Glu            
   1               5                   10                  15             

gag gcc aaa gtg acc gtt cct cgt tcc aca aga tta tca gca act ggt        455
Glu Ala Lys Val Thr Val Pro Arg Ser Thr Arg Leu Ser Ala Thr Gly           
                20                  25                  30                

cca gtt gaa tgt gat tta tct ggt ttc caa gtt ttg aac tct cca ctt        503
Pro Val Glu Cys Asp Leu Ser Gly Phe Gln Val Leu Asn Ser Pro Leu           
            35                  40                  45                    

ttc aac aag ggc act gca ttc act att gca gaa aga gaa gca ttt ggt        551
Phe Asn Lys Gly Thr Ala Phe Thr Ile Ala Glu Arg Glu Ala Phe Gly           
        50                  55                  60                        

tta aac ggg tta ctt cct ccg gtt gta aac act cta gaa gaa caa gtt        599
Leu Asn Gly Leu Leu Pro Pro Val Val Asn Thr Leu Glu Glu Gln Val           
    65                  70                  75                            

gag aga agc tat aag caa cta cat ttt ctc aag act cca ttg gca aag        647
Glu Arg Ser Tyr Lys Gln Leu His Phe Leu Lys Thr Pro Leu Ala Lys           
80                  85                  90                  95            

aat gac ttt tgc acg tca ttg aga ttg caa aac aag gtt ctg ttt tat        695
Asn Asp Phe Cys Thr Ser Leu Arg Leu Gln Asn Lys Val Leu Phe Tyr           
                100                 105                 110               

agg tta gtc aag gaa cac att aag gag ttg att cca att gtg tat aca        743
Arg Leu Val Lys Glu His Ile Lys Glu Leu Ile Pro Ile Val Tyr Thr           
            115                 120                 125                   

cca aca gag ggt gat gca att atc gct tat tct gac aga ttc aga aaa        791
Pro Thr Glu Gly Asp Ala Ile Ile Ala Tyr Ser Asp Arg Phe Arg Lys           
        130                 135                 140                       

cca gag ggg tta ttc ctt gat att aca aga cca aat gaa att gat caa        839
Pro Glu Gly Leu Phe Leu Asp Ile Thr Arg Pro Asn Glu Ile Asp Gln           
    145                 150                 155                           

aga ctg gaa cag ttt gga gaa gat aaa gat gtg gat tac att gtt ata        887
Arg Leu Glu Gln Phe Gly Glu Asp Lys Asp Val Asp Tyr Ile Val Ile           
160                 165                 170                 175           

aca gat tct gaa ggt att cta ggt att ggt gac caa ggt gtt ggc ggt        935
Thr Asp Ser Glu Gly Ile Leu Gly Ile Gly Asp Gln Gly Val Gly Gly           
                180                 185                 190               

gtc aga atc aca att gca aag gct gct cta atg act gta tgt gct ggt        983
Val Arg Ile Thr Ile Ala Lys Ala Ala Leu Met Thr Val Cys Ala Gly           
            195                 200                 205                   

ttg cat cca ggt aga gtt gtc tct tgt gtc ttg gac gtt ggc act aac       1031
Leu His Pro Gly Arg Val Val Ser Cys Val Leu Asp Val Gly Thr Asn           
        210                 215                 220                       

aat gtg aaa ttg cta gaa gat gat cta tat ctt ggt aac aga ttc cca       1079
Asn Val Lys Leu Leu Glu Asp Asp Leu Tyr Leu Gly Asn Arg Phe Pro           
    225                 230                 235                           

aga gtt aga ggt aag gag tat gac gat ttt gtg aat aaa act att cgt       1127
Arg Val Arg Gly Lys Glu Tyr Asp Asp Phe Val Asn Lys Thr Ile Arg           
240                 245                 250                 255           

gca atg aag aag aga ttt cca agt gct gtt att cat ttt gaa gat ttt       1175
Ala Met Lys Lys Arg Phe Pro Ser Ala Val Ile His Phe Glu Asp Phe           
                260                 265                 270               

ggt gtt aca act gct aga cct gtg ttg gaa aga ttc aga gat gaa att       1223
Gly Val Thr Thr Ala Arg Pro Val Leu Glu Arg Phe Arg Asp Glu Ile           
            275                 280                 285                   

cct tgc ttt aat gat gac atc caa ggt acc ggc gct gtt gtc atg gct       1271
Pro Cys Phe Asn Asp Asp Ile Gln Gly Thr Gly Ala Val Val Met Ala           
        290                 295                 300                       

tcg atg gcg gct gct ctt aaa tta acc cat aga aat cta cta gac tcc       1319
Ser Met Ala Ala Ala Leu Lys Leu Thr His Arg Asn Leu Leu Asp Ser           
    305                 310                 315                           

aaa gtt ttg att tat ggg gct ggt tca gcc ggt ttg ggt att gcc gat       1367
Lys Val Leu Ile Tyr Gly Ala Gly Ser Ala Gly Leu Gly Ile Ala Asp           
320                 325                 330                 335           

caa att gtt aat cat atg gtt agc cat ggt gcg act aaa gaa gaa gct       1415
Gln Ile Val Asn His Met Val Ser His Gly Ala Thr Lys Glu Glu Ala           
                340                 345                 350               

aga agg aag att tac tgt atg gac agg tat ggt ttg att tta aaa ggt       1463
Arg Arg Lys Ile Tyr Cys Met Asp Arg Tyr Gly Leu Ile Leu Lys Gly           
            355                 360                 365                   

atg act tcg aat tct cct gct caa gaa gat tat gct cat gat cca aaa       1511
Met Thr Ser Asn Ser Pro Ala Gln Glu Asp Tyr Ala His Asp Pro Lys           
        370                 375                 380                       

gat tgg gaa aat att tca acc act tcg tta gtg gat gtt atc gaa aaa       1559
Asp Trp Glu Asn Ile Ser Thr Thr Ser Leu Val Asp Val Ile Glu Lys           
    385                 390                 395                           

gtc aag cct act act tta gtt ggg tgc tcc acg caa gcg ggc gct ttc       1607
Val Lys Pro Thr Thr Leu Val Gly Cys Ser Thr Gln Ala Gly Ala Phe           
400                 405                 410                 415           

aat gaa gaa gtc atc aaa aca atg tat aaa cat aat cca aga cca atg       1655
Asn Glu Glu Val Ile Lys Thr Met Tyr Lys His Asn Pro Arg Pro Met           
                420                 425                 430               

att ttc cca ttg tcc aac cca act aga tta cat gag tgt ttc cct gaa       1703
Ile Phe Pro Leu Ser Asn Pro Thr Arg Leu His Glu Cys Phe Pro Glu           
            435                 440                 445                   

gac gca ctt aaa tgg acc gat ttc aac gct tta gtt gcc act ggt tct       1751
Asp Ala Leu Lys Trp Thr Asp Phe Asn Ala Leu Val Ala Thr Gly Ser           
        450                 455                 460                       

cct ttc cca cct gtt gaa ggt cat gtt att tct gaa aat aac aat tgt       1799
Pro Phe Pro Pro Val Glu Gly His Val Ile Ser Glu Asn Asn Asn Cys           
    465                 470                 475                           

ttt gcc ttc ccg ggt att ggt cta ggt gca gtg ctc gct aga act act       1847
Phe Ala Phe Pro Gly Ile Gly Leu Gly Ala Val Leu Ala Arg Thr Thr           
480                 485                 490                 495           

agg ata tca gac aac atg att tcg gct gcc gtt gac gag cta gct tct       1895
Arg Ile Ser Asp Asn Met Ile Ser Ala Ala Val Asp Glu Leu Ala Ser           
                500                 505                 510               

ctt tct cca gct caa aaa gat cct aaa ttg ggc ctt tta cct cca att       1943
Leu Ser Pro Ala Gln Lys Asp Pro Lys Leu Gly Leu Leu Pro Pro Ile           
            515                 520                 525                   

gag gaa atc gac gag acc tct gca aga atc gca act gca gtt atc ttg       1991
Glu Glu Ile Asp Glu Thr Ser Ala Arg Ile Ala Thr Ala Val Ile Leu           
        530                 535                 540                       

aag gct gtc gag gaa gga ttt gca aga gta gaa gaa gaa gat tct cca       2039
Lys Ala Val Glu Glu Gly Phe Ala Arg Val Glu Glu Glu Asp Ser Pro           
    545                 550                 555                           

tta ggt ggt aaa gtt aaa att cca aga gag ttt gat cca tgt cta aga       2087
Leu Gly Gly Lys Val Lys Ile Pro Arg Glu Phe Asp Pro Cys Leu Arg           
560                 565                 570                 575           

tgg gtt aaa gaa cag atg tgg cat cca att tac aga cca atg atc aaa       2135
Trp Val Lys Glu Gln Met Trp His Pro Ile Tyr Arg Pro Met Ile Lys           
                580                 585                 590               

gtc gca cac tca gac aat att cat act cac caa tac taa tggacacata        2184
Val Ala His Ser Asp Asn Ile His Thr His Gln Tyr                           
            595                 600                                       

cacattatca aatgcattta ttcctaatat cacactaaaa cgtattatat aattttaatc     2244

tttatagact tcatagcacc aattggattt gctttctttc agaataccgc acttaatctc     2304

aatgtacgta acgtaggcaa aatctgtcga taaggatctg tatgccgtaa acggaaactc     2364

caagcgccca gaaaacttac attatattct tgccagtttc atctcaccag ccagtcacag     2424

tttaaaaggt ttgattgcgt ttcttgtttc gtcggattca gtgctaattg gtaacgcact     2484

gtaccgccac accaaagcaa aaatgcagaa acaaacaaca atgagtgtat gtttaccaac     2544

tttggttttg aaagaaaa                                                   2562


<210>  40
<211>  603
<212>  PRT
<213>  Issatchenkia orientalis

<400>  40

Met Thr Lys Asp Cys Cys Glu Val Thr Lys Ile Pro Val Gly Glu Glu 
1               5                   10                  15      


Ala Lys Val Thr Val Pro Arg Ser Thr Arg Leu Ser Ala Thr Gly Pro 
            20                  25                  30          


Val Glu Cys Asp Leu Ser Gly Phe Gln Val Leu Asn Ser Pro Leu Phe 
        35                  40                  45              


Asn Lys Gly Thr Ala Phe Thr Ile Ala Glu Arg Glu Ala Phe Gly Leu 
    50                  55                  60                  


Asn Gly Leu Leu Pro Pro Val Val Asn Thr Leu Glu Glu Gln Val Glu 
65                  70                  75                  80  


Arg Ser Tyr Lys Gln Leu His Phe Leu Lys Thr Pro Leu Ala Lys Asn 
                85                  90                  95      


Asp Phe Cys Thr Ser Leu Arg Leu Gln Asn Lys Val Leu Phe Tyr Arg 
            100                 105                 110         


Leu Val Lys Glu His Ile Lys Glu Leu Ile Pro Ile Val Tyr Thr Pro 
        115                 120                 125             


Thr Glu Gly Asp Ala Ile Ile Ala Tyr Ser Asp Arg Phe Arg Lys Pro 
    130                 135                 140                 


Glu Gly Leu Phe Leu Asp Ile Thr Arg Pro Asn Glu Ile Asp Gln Arg 
145                 150                 155                 160 


Leu Glu Gln Phe Gly Glu Asp Lys Asp Val Asp Tyr Ile Val Ile Thr 
                165                 170                 175     


Asp Ser Glu Gly Ile Leu Gly Ile Gly Asp Gln Gly Val Gly Gly Val 
            180                 185                 190         


Arg Ile Thr Ile Ala Lys Ala Ala Leu Met Thr Val Cys Ala Gly Leu 
        195                 200                 205             


His Pro Gly Arg Val Val Ser Cys Val Leu Asp Val Gly Thr Asn Asn 
    210                 215                 220                 


Val Lys Leu Leu Glu Asp Asp Leu Tyr Leu Gly Asn Arg Phe Pro Arg 
225                 230                 235                 240 


Val Arg Gly Lys Glu Tyr Asp Asp Phe Val Asn Lys Thr Ile Arg Ala 
                245                 250                 255     


Met Lys Lys Arg Phe Pro Ser Ala Val Ile His Phe Glu Asp Phe Gly 
            260                 265                 270         


Val Thr Thr Ala Arg Pro Val Leu Glu Arg Phe Arg Asp Glu Ile Pro 
        275                 280                 285             


Cys Phe Asn Asp Asp Ile Gln Gly Thr Gly Ala Val Val Met Ala Ser 
    290                 295                 300                 


Met Ala Ala Ala Leu Lys Leu Thr His Arg Asn Leu Leu Asp Ser Lys 
305                 310                 315                 320 


Val Leu Ile Tyr Gly Ala Gly Ser Ala Gly Leu Gly Ile Ala Asp Gln 
                325                 330                 335     


Ile Val Asn His Met Val Ser His Gly Ala Thr Lys Glu Glu Ala Arg 
            340                 345                 350         


Arg Lys Ile Tyr Cys Met Asp Arg Tyr Gly Leu Ile Leu Lys Gly Met 
        355                 360                 365             


Thr Ser Asn Ser Pro Ala Gln Glu Asp Tyr Ala His Asp Pro Lys Asp 
    370                 375                 380                 


Trp Glu Asn Ile Ser Thr Thr Ser Leu Val Asp Val Ile Glu Lys Val 
385                 390                 395                 400 


Lys Pro Thr Thr Leu Val Gly Cys Ser Thr Gln Ala Gly Ala Phe Asn 
                405                 410                 415     


Glu Glu Val Ile Lys Thr Met Tyr Lys His Asn Pro Arg Pro Met Ile 
            420                 425                 430         


Phe Pro Leu Ser Asn Pro Thr Arg Leu His Glu Cys Phe Pro Glu Asp 
        435                 440                 445             


Ala Leu Lys Trp Thr Asp Phe Asn Ala Leu Val Ala Thr Gly Ser Pro 
    450                 455                 460                 


Phe Pro Pro Val Glu Gly His Val Ile Ser Glu Asn Asn Asn Cys Phe 
465                 470                 475                 480 


Ala Phe Pro Gly Ile Gly Leu Gly Ala Val Leu Ala Arg Thr Thr Arg 
                485                 490                 495     


Ile Ser Asp Asn Met Ile Ser Ala Ala Val Asp Glu Leu Ala Ser Leu 
            500                 505                 510         


Ser Pro Ala Gln Lys Asp Pro Lys Leu Gly Leu Leu Pro Pro Ile Glu 
        515                 520                 525             


Glu Ile Asp Glu Thr Ser Ala Arg Ile Ala Thr Ala Val Ile Leu Lys 
    530                 535                 540                 


Ala Val Glu Glu Gly Phe Ala Arg Val Glu Glu Glu Asp Ser Pro Leu 
545                 550                 555                 560 


Gly Gly Lys Val Lys Ile Pro Arg Glu Phe Asp Pro Cys Leu Arg Trp 
                565                 570                 575     


Val Lys Glu Gln Met Trp His Pro Ile Tyr Arg Pro Met Ile Lys Val 
            580                 585                 590         


Ala His Ser Asp Asn Ile His Thr His Gln Tyr 
        595                 600             


<210>  41
<211>  3743
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1010)..(2746)

<400>  41
atttgtgtgt gtgtgtcagt gtaagtgtta gtatacttgt tttcctcttt cccctagagt       60

tggtggtgtg ttttgttgga acgtacatta gatgcataat gcgtgacacc gccatgatgg      120

ttgtattcta ccaatgagac atggccgctg atcctgttgt gtgggtcatg ggacatcacc      180

tcttgggggg gattctccta taattggcac cgtgtatgcc tcaaccacta acttccaccc      240

tataactgaa tatattacat aagcaaatct actttttgtt tgtgttgatc gccatcgttg      300

aaattcgcgc aacttctggt ggctcaacgc tgctgttcta tcggtatcct aagagatgtc      360

tttgccctga gtctagggta aactatccac cttcgttgct gtttgactag acagctacta      420

actttacggt agtaaatgaa taacggctcg ctctcatgat cacttctcta catcacccta      480

acaagtgtat tatttttttt tcaggtgggt gttgctgttg gtgctagcct tagtgccctc      540

gttaatagtt gaacaaacac tggcatttgg agtataatga aaagggatca ctaccccccg      600

cttcctgttc cgcttctccc ttccggaaaa accacccacc ctttcttttc ccccactaat      660

gtatgaattt ttccgttccc aggggaatgg cccacttggt tctctgttaa cccacacaat      720

tttgacgcat cccacacacc tttttttttt ctaccccaca ctttcccttg aaaaatctcc      780

aatttgaact ggcaattttc accccccacc acttgcattc attagtgagt caatccatcc      840

cgcggtcgga gattcggaat ccacctactg gtaatctgta atctatattc ccgctgaccc      900

tttataaatg aactattgtc gtcaattgcg gtagtgctcc aacaaattgt aaggaccttc      960

tttaaccttt tcgattcaat ccatctccac ataaacctag ttgcacaca atg tta ctc     1018
                                                      Met Leu Leu         
                                                      1                   

aga tca cta aac tct tct gct cgt tgt gtc aaa caa aca acc aga aca       1066
Arg Ser Leu Asn Ser Ser Ala Arg Cys Val Lys Gln Thr Thr Arg Thr           
    5                   10                  15                            

aag gtt agg tat ctc agc cac gtc agt ggt gca agc atg gcg aaa cct       1114
Lys Val Arg Tyr Leu Ser His Val Ser Gly Ala Ser Met Ala Lys Pro           
20                  25                  30                  35            

aca ttg aag aac aac tcg aga gaa tcc aac aaa tcc aga aac tat cta       1162
Thr Leu Lys Asn Asn Ser Arg Glu Ser Asn Lys Ser Arg Asn Tyr Leu           
                40                  45                  50                

att gct gct gtg aca gca ttg gct gta tca acc tca att gga gtt gcc       1210
Ile Ala Ala Val Thr Ala Leu Ala Val Ser Thr Ser Ile Gly Val Ala           
            55                  60                  65                    

gta cat gtg aag gac ccc ttg tat aac gat gct acc ggc agt gat tct       1258
Val His Val Lys Asp Pro Leu Tyr Asn Asp Ala Thr Gly Ser Asp Ser           
        70                  75                  80                        

ccg aga agt ata tct gtt gac gag ttt gtc aag cat aat tca caa aac       1306
Pro Arg Ser Ile Ser Val Asp Glu Phe Val Lys His Asn Ser Gln Asn           
    85                  90                  95                            

gac tgt tgg att gca atc aat ggc aag gtt tat gat ttc act gat ttt       1354
Asp Cys Trp Ile Ala Ile Asn Gly Lys Val Tyr Asp Phe Thr Asp Phe           
100                 105                 110                 115           

att cca aac cat cca ggt ggg gta cct cca tta gtt aat cat gct ggt       1402
Ile Pro Asn His Pro Gly Gly Val Pro Pro Leu Val Asn His Ala Gly           
                120                 125                 130               

tat gat ggt act aaa ctt tat gag aaa ttg cat cca aaa ggt aca att       1450
Tyr Asp Gly Thr Lys Leu Tyr Glu Lys Leu His Pro Lys Gly Thr Ile           
            135                 140                 145                   

gag aaa ttc ttg cca aag gat aag ttt ctg ggt gtg tta gat ggt gaa       1498
Glu Lys Phe Leu Pro Lys Asp Lys Phe Leu Gly Val Leu Asp Gly Glu           
        150                 155                 160                       

gcg cca aaa ttg gaa gca gac tat ttg gtg gac gat gat gaa caa gag       1546
Ala Pro Lys Leu Glu Ala Asp Tyr Leu Val Asp Asp Asp Glu Gln Glu           
    165                 170                 175                           

aga ctg gat tat ttg aac aac tta cct cct ttg tca tct att cag aat       1594
Arg Leu Asp Tyr Leu Asn Asn Leu Pro Pro Leu Ser Ser Ile Gln Asn           
180                 185                 190                 195           

gtt tat gat ttc gaa tac ttg gcc aag aag att tta cct aaa gat gcc       1642
Val Tyr Asp Phe Glu Tyr Leu Ala Lys Lys Ile Leu Pro Lys Asp Ala           
                200                 205                 210               

tgg gca tat tat tct tgt ggt gcc gat gat gaa atc aca atg aga gaa       1690
Trp Ala Tyr Tyr Ser Cys Gly Ala Asp Asp Glu Ile Thr Met Arg Glu           
            215                 220                 225                   

aac cat tat gct tat caa aga gtt tat ttc aga cca aga att tgt gtt       1738
Asn His Tyr Ala Tyr Gln Arg Val Tyr Phe Arg Pro Arg Ile Cys Val           
        230                 235                 240                       

gat gtc aag gaa gtt gat act tct tat gaa atg tta ggc act aaa acc       1786
Asp Val Lys Glu Val Asp Thr Ser Tyr Glu Met Leu Gly Thr Lys Thr           
    245                 250                 255                           

tct gtt cct ttt tat gta tct gcc acc gct ttg gct aaa tta ggc cat       1834
Ser Val Pro Phe Tyr Val Ser Ala Thr Ala Leu Ala Lys Leu Gly His           
260                 265                 270                 275           

cct gat ggt gaa tgc tca att gct aga ggc gct ggt aag gaa ggt gtc       1882
Pro Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys Glu Gly Val           
                280                 285                 290               

gtt caa atg att tcg acc ctt tcc tca atg tca tta gat gaa att gcc       1930
Val Gln Met Ile Ser Thr Leu Ser Ser Met Ser Leu Asp Glu Ile Ala           
            295                 300                 305                   

gct gct aga att cca ggt gca acc caa tgg ttc caa tta tac att aat       1978
Ala Ala Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu Tyr Ile Asn           
        310                 315                 320                       

gag gat aga aat gtc gct aaa ggt ctg gtc aaa cat gca gaa gac ttg       2026
Glu Asp Arg Asn Val Ala Lys Gly Leu Val Lys His Ala Glu Asp Leu           
    325                 330                 335                           

ggt atg aag gct atc ttt ata act gtt gat gct cct tct cta ggt aac       2074
Gly Met Lys Ala Ile Phe Ile Thr Val Asp Ala Pro Ser Leu Gly Asn           
340                 345                 350                 355           

aga gaa aag gat aaa aga tta aag ttt gtt aat gac acc gat gtc gat       2122
Arg Glu Lys Asp Lys Arg Leu Lys Phe Val Asn Asp Thr Asp Val Asp           
                360                 365                 370               

ttg ggt gat tcc gca gat cga aac agt ggt gct tca aag gca cta tct       2170
Leu Gly Asp Ser Ala Asp Arg Asn Ser Gly Ala Ser Lys Ala Leu Ser           
            375                 380                 385                   

tcg ttc att gat gct tct gtc tct tgg aat gac gtc aaa gcg gtc aag       2218
Ser Phe Ile Asp Ala Ser Val Ser Trp Asn Asp Val Lys Ala Val Lys           
        390                 395                 400                       

tcg tgg act aaa ttg cct gtc tta gtt aaa ggt gtt caa aca gtt gaa       2266
Ser Trp Thr Lys Leu Pro Val Leu Val Lys Gly Val Gln Thr Val Glu           
    405                 410                 415                           

gac gtt att gaa gct tac gat gct ggt tgt caa ggt gtt gtt ttg tca       2314
Asp Val Ile Glu Ala Tyr Asp Ala Gly Cys Gln Gly Val Val Leu Ser           
420                 425                 430                 435           

aac cac ggt ggt agg caa cta gat act gct cct cct cca atc gaa tta       2362
Asn His Gly Gly Arg Gln Leu Asp Thr Ala Pro Pro Pro Ile Glu Leu           
                440                 445                 450               

tta gct gaa act gtt cca act ttg aag aga ttg ggt aaa tta aga cca       2410
Leu Ala Glu Thr Val Pro Thr Leu Lys Arg Leu Gly Lys Leu Arg Pro           
            455                 460                 465                   

gat ttt gaa att tta att gac ggt ggt gtc aaa aga ggt acc gat att       2458
Asp Phe Glu Ile Leu Ile Asp Gly Gly Val Lys Arg Gly Thr Asp Ile           
        470                 475                 480                       

ttg aaa gca gtc gca atc ggt ggc caa gat gtc aga gtt tca gtt ggt       2506
Leu Lys Ala Val Ala Ile Gly Gly Gln Asp Val Arg Val Ser Val Gly           
    485                 490                 495                           

atg ggt aga cct ttc tta tat gcc aac tct tgc tat ggt gaa gca ggt       2554
Met Gly Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly Glu Ala Gly           
500                 505                 510                 515           

gtt aga aaa tta att caa aat cta aag gat gaa tta gaa atg gat atg       2602
Val Arg Lys Leu Ile Gln Asn Leu Lys Asp Glu Leu Glu Met Asp Met           
                520                 525                 530               

aga ttg ttg ggt gtc act aaa atg gac cag cta tct tcg aaa cat gtc       2650
Arg Leu Leu Gly Val Thr Lys Met Asp Gln Leu Ser Ser Lys His Val           
            535                 540                 545                   

gat act aaa cgt ttg att ggt aga gat gcg atc aac tat ttg tat gat       2698
Asp Thr Lys Arg Leu Ile Gly Arg Asp Ala Ile Asn Tyr Leu Tyr Asp           
        550                 555                 560                       

aat gta tac agc cca atc gaa acc gtt aaa ttc aac aat gaa gat tga       2746
Asn Val Tyr Ser Pro Ile Glu Thr Val Lys Phe Asn Asn Glu Asp               
    565                 570                 575                           

ttgttggaaa tatattattc ataaaggcga aaacattccc ttggtatttt attccaaatt     2806

tatgatacat agacgtattt tttatatata aagttatatt attaatgatt caagaaaaag     2866

ttcaaataaa ctaatggatc aacctatttc gaccctttct tcattgctac ttcttcctta     2926

agcaacagat gattaagtag atactgtttt tttagccaat agtatctcgc cgaggagtta     2986

tacttgacta gctcttgctc aagaatcttc ctaagacgta ctagcctagc atagtaatct     3046

gtttgtttct gtattgtttg ttctaactgt tctacagtca ttgaatcaat atctccaatg     3106

tcttcgacgt tgacaacttt cccccccttg gcagcattct cttttttgtt ggaatacgac     3166

attaaagatt ccttgatttt ctgggtacct tcaatgacca ttgagggatt aaatttgatt     3226

tctttgattt tataatggtc ggctattagc tcttccactt cgtcatcatg atcatcagat     3286

atgtcacgtt gccttttcaa tttattaaaa ttgtttatca gtttattgtg atcttgtatc     3346

aattcattgc gtactctttt ctcaatatca aaagctattt tcttcccgct agactcaaaa     3406

tcaactctga agtcattttc tcgctggaat tcatgtattt catggattaa ttctctattg     3466

atattctcat atgcatcctg taaactgttg ccgttgatat tatgaaccgc ctttaaatgt     3526

ttcaataagg catctgctct agtaaatgcc ttcagacatt caggtaataa acagtaaaat     3586

ggcttctcgg ctgtatgcgt cctaatatga gacaatagcg caaatctact gtgttgtgga     3646

gtaccatatc ttggacaatt tccccactta catgaatggt ctgtgttggg ttgtttaaaa     3706

tgattagtat ttatgtgatt aaccatctca tccattt                              3743


<210>  42
<211>  578
<212>  PRT
<213>  Issatchenkia orientalis

<400>  42

Met Leu Leu Arg Ser Leu Asn Ser Ser Ala Arg Cys Val Lys Gln Thr 
1               5                   10                  15      


Thr Arg Thr Lys Val Arg Tyr Leu Ser His Val Ser Gly Ala Ser Met 
            20                  25                  30          


Ala Lys Pro Thr Leu Lys Asn Asn Ser Arg Glu Ser Asn Lys Ser Arg 
        35                  40                  45              


Asn Tyr Leu Ile Ala Ala Val Thr Ala Leu Ala Val Ser Thr Ser Ile 
    50                  55                  60                  


Gly Val Ala Val His Val Lys Asp Pro Leu Tyr Asn Asp Ala Thr Gly 
65                  70                  75                  80  


Ser Asp Ser Pro Arg Ser Ile Ser Val Asp Glu Phe Val Lys His Asn 
                85                  90                  95      


Ser Gln Asn Asp Cys Trp Ile Ala Ile Asn Gly Lys Val Tyr Asp Phe 
            100                 105                 110         


Thr Asp Phe Ile Pro Asn His Pro Gly Gly Val Pro Pro Leu Val Asn 
        115                 120                 125             


His Ala Gly Tyr Asp Gly Thr Lys Leu Tyr Glu Lys Leu His Pro Lys 
    130                 135                 140                 


Gly Thr Ile Glu Lys Phe Leu Pro Lys Asp Lys Phe Leu Gly Val Leu 
145                 150                 155                 160 


Asp Gly Glu Ala Pro Lys Leu Glu Ala Asp Tyr Leu Val Asp Asp Asp 
                165                 170                 175     


Glu Gln Glu Arg Leu Asp Tyr Leu Asn Asn Leu Pro Pro Leu Ser Ser 
            180                 185                 190         


Ile Gln Asn Val Tyr Asp Phe Glu Tyr Leu Ala Lys Lys Ile Leu Pro 
        195                 200                 205             


Lys Asp Ala Trp Ala Tyr Tyr Ser Cys Gly Ala Asp Asp Glu Ile Thr 
    210                 215                 220                 


Met Arg Glu Asn His Tyr Ala Tyr Gln Arg Val Tyr Phe Arg Pro Arg 
225                 230                 235                 240 


Ile Cys Val Asp Val Lys Glu Val Asp Thr Ser Tyr Glu Met Leu Gly 
                245                 250                 255     


Thr Lys Thr Ser Val Pro Phe Tyr Val Ser Ala Thr Ala Leu Ala Lys 
            260                 265                 270         


Leu Gly His Pro Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys 
        275                 280                 285             


Glu Gly Val Val Gln Met Ile Ser Thr Leu Ser Ser Met Ser Leu Asp 
    290                 295                 300                 


Glu Ile Ala Ala Ala Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu 
305                 310                 315                 320 


Tyr Ile Asn Glu Asp Arg Asn Val Ala Lys Gly Leu Val Lys His Ala 
                325                 330                 335     


Glu Asp Leu Gly Met Lys Ala Ile Phe Ile Thr Val Asp Ala Pro Ser 
            340                 345                 350         


Leu Gly Asn Arg Glu Lys Asp Lys Arg Leu Lys Phe Val Asn Asp Thr 
        355                 360                 365             


Asp Val Asp Leu Gly Asp Ser Ala Asp Arg Asn Ser Gly Ala Ser Lys 
    370                 375                 380                 


Ala Leu Ser Ser Phe Ile Asp Ala Ser Val Ser Trp Asn Asp Val Lys 
385                 390                 395                 400 


Ala Val Lys Ser Trp Thr Lys Leu Pro Val Leu Val Lys Gly Val Gln 
                405                 410                 415     


Thr Val Glu Asp Val Ile Glu Ala Tyr Asp Ala Gly Cys Gln Gly Val 
            420                 425                 430         


Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ala Pro Pro Pro 
        435                 440                 445             


Ile Glu Leu Leu Ala Glu Thr Val Pro Thr Leu Lys Arg Leu Gly Lys 
    450                 455                 460                 


Leu Arg Pro Asp Phe Glu Ile Leu Ile Asp Gly Gly Val Lys Arg Gly 
465                 470                 475                 480 


Thr Asp Ile Leu Lys Ala Val Ala Ile Gly Gly Gln Asp Val Arg Val 
                485                 490                 495     


Ser Val Gly Met Gly Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly 
            500                 505                 510         


Glu Ala Gly Val Arg Lys Leu Ile Gln Asn Leu Lys Asp Glu Leu Glu 
        515                 520                 525             


Met Asp Met Arg Leu Leu Gly Val Thr Lys Met Asp Gln Leu Ser Ser 
    530                 535                 540                 


Lys His Val Asp Thr Lys Arg Leu Ile Gly Arg Asp Ala Ile Asn Tyr 
545                 550                 555                 560 


Leu Tyr Asp Asn Val Tyr Ser Pro Ile Glu Thr Val Lys Phe Asn Asn 
                565                 570                 575     


Glu Asp 
        


<210>  43
<211>  3695
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1001)..(2698)

<400>  43
ctcgtttcct gtgatatgtt tgccgtgcgc ctggtcgatt ttcaccttct ttgaaatccg       60

agttcgggaa tgcaattggg aaaaagccaa ggagaaagaa aacaaaaaga gagttgcgta      120

gaaactcgga atgctcgaag aaaccagaca gttgatggct ggtttccgtt ttgaggacgc      180

ttggtgtgtg taacttggat ttgcacacta gagccgtctc tgcattgtat taaggtgtaa      240

ggacggtgaa tcatcgcgat ggagcggggt tttttctttt ggcaggtttt tccgcggaag      300

gcgagagggc gagagggggg gggggtgtat gtagttcata tttcggcatt actacaagga      360

tgtttccgta cattgcatgg tactggggtt ctccttttct tgcacatctc cataaactaa      420

atatcaatag atgtatccgt ttggaatctc atgacttttg gtgtgtggtc tgtgtcttcc      480

cagttatcta cttgagtgat tatgatccag ttttcaccat tggttacata ccaaacagag      540

aacttatacg caccagaacg ccttttgtgt ctttttgttt ctcaagtatt tctatcagtt      600

tccttcatgt atcccgggac tccattgtcc tcggtagtgc ctaccaattt aatgtttgac      660

tccttgcgtt ttctcctgtc gcggacaaac ggtgcggctc ccccgatgat tcacgtaata      720

agccggagtc aaccacagag gtcccctatg actcaacaag gcctcgtaga aactcggctt      780

ctcggagaaa gagtcttttc tttttcactg gaaaatattt ttttttcctt tatattcttt      840

tgaaccaaaa tgtggctact ataaaagtgc ctttattccc cagcttttct agcatgattg      900

agtcaccttc cacaatgagt cttctttatt gttagtattg tgaatattat ccgtgcagtt      960

ttcaagaacg taaatcaaca gcagtgataa taccttcaaa atg tta aga tcc cag       1015
                                            Met Leu Arg Ser Gln           
                                            1               5             

ttc aaa aac att ttg aaa aat gtt aac aag aac cat tct cta agg aga       1063
Phe Lys Asn Ile Leu Lys Asn Val Asn Lys Asn His Ser Leu Arg Arg           
                10                  15                  20                

act ttt act tcc agc acc tca aag gct gga aaa aat gct tca tac aat       1111
Thr Phe Thr Ser Ser Thr Ser Lys Ala Gly Lys Asn Ala Ser Tyr Asn           
            25                  30                  35                    

gcc aag att ata tct gca acc gtg gcc tcg att gtt gca gca gct ggc       1159
Ala Lys Ile Ile Ser Ala Thr Val Ala Ser Ile Val Ala Ala Ala Gly           
        40                  45                  50                        

tct tat atg ttg gtc cag cct tca cta gct aat gat gag gca cag tct       1207
Ser Tyr Met Leu Val Gln Pro Ser Leu Ala Asn Asp Glu Ala Gln Ser           
    55                  60                  65                            

gct aat cca act agg aag atc tct gtt gac gaa ttt gtt aaa cac aac       1255
Ala Asn Pro Thr Arg Lys Ile Ser Val Asp Glu Phe Val Lys His Asn           
70                  75                  80                  85            

cat gcc gat gat tgt tgg atc act gtt aac ggt aac gtc tat gac ttg       1303
His Ala Asp Asp Cys Trp Ile Thr Val Asn Gly Asn Val Tyr Asp Leu           
                90                  95                  100               

act gat ttc att tca atg cat cca ggt ggt act acc cca ttg att caa       1351
Thr Asp Phe Ile Ser Met His Pro Gly Gly Thr Thr Pro Leu Ile Gln           
            105                 110                 115                   

aat gca ggt cac gac gca act gaa att tac aac aag att cat cca aag       1399
Asn Ala Gly His Asp Ala Thr Glu Ile Tyr Asn Lys Ile His Pro Lys           
        120                 125                 130                       

ggt aca atc gag aac ttc tta cca aag gaa aag caa ttg ggt gtt ttg       1447
Gly Thr Ile Glu Asn Phe Leu Pro Lys Glu Lys Gln Leu Gly Val Leu           
    135                 140                 145                           

gat ggt gaa gct cct aaa atc gaa gtt gtg ctt gac gaa aag gag aaa       1495
Asp Gly Glu Ala Pro Lys Ile Glu Val Val Leu Asp Glu Lys Glu Lys           
150                 155                 160                 165           

cac aga ttg gag ttg ttg aat cat ctc cct gct ctt tcc aga att caa       1543
His Arg Leu Glu Leu Leu Asn His Leu Pro Ala Leu Ser Arg Ile Gln           
                170                 175                 180               

aac att tat gat ttc gaa cat att gct tct aga gtt ttg agc gac caa       1591
Asn Ile Tyr Asp Phe Glu His Ile Ala Ser Arg Val Leu Ser Asp Gln           
            185                 190                 195                   

gca tgg aac tac tat tca tgt ggt gcc gaa gat gaa atc acc ttg agg       1639
Ala Trp Asn Tyr Tyr Ser Cys Gly Ala Glu Asp Glu Ile Thr Leu Arg           
        200                 205                 210                       

gaa aat cat tat gct tac caa aga atc tac ttt aag cca aaa tgt tgt       1687
Glu Asn His Tyr Ala Tyr Gln Arg Ile Tyr Phe Lys Pro Lys Cys Cys           
    215                 220                 225                           

gtc aat gtt gca gaa gtt gat acc tct cat gaa att tta ggt aca aaa       1735
Val Asn Val Ala Glu Val Asp Thr Ser His Glu Ile Leu Gly Thr Lys           
230                 235                 240                 245           

gct tct gtt cct ttc tac gtt tcc gca gcc gct tct gca aag ttg ggg       1783
Ala Ser Val Pro Phe Tyr Val Ser Ala Ala Ala Ser Ala Lys Leu Gly           
                250                 255                 260               

cac gag gat ggt gaa tgt tcc att gct aga ggt gca ggt aag gaa ggc       1831
His Glu Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys Glu Gly           
            265                 270                 275                   

gtt att caa atg att tct tcc ttc tct tcc aac tct ttg gag gaa att       1879
Val Ile Gln Met Ile Ser Ser Phe Ser Ser Asn Ser Leu Glu Glu Ile           
        280                 285                 290                       

gca gaa tcc aga att cct ggt gca aca caa tgg ttt caa tta tac gtt       1927
Ala Glu Ser Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu Tyr Val           
    295                 300                 305                           

aat gaa gac aag gat gtt gtg aag aag act tta aaa agg gcc gaa aac       1975
Asn Glu Asp Lys Asp Val Val Lys Lys Thr Leu Lys Arg Ala Glu Asn           
310                 315                 320                 325           

ttg ggt atg aag gcc atc ttt gtc act gtg gac gct gct agt aga ggt       2023
Leu Gly Met Lys Ala Ile Phe Val Thr Val Asp Ala Ala Ser Arg Gly           
                330                 335                 340               

aat aga gaa aaa gac att aga atg aga att acc gaa gat aca gat gag       2071
Asn Arg Glu Lys Asp Ile Arg Met Arg Ile Thr Glu Asp Thr Asp Glu           
            345                 350                 355                   

tta atc gac gat tct tct gtt aga gct ggt tct acc tct ggt gca ttg       2119
Leu Ile Asp Asp Ser Ser Val Arg Ala Gly Ser Thr Ser Gly Ala Leu           
        360                 365                 370                       

cca gct ttc att gac aag agg ctg act tgg gat gaa gtt aag gat atc       2167
Pro Ala Phe Ile Asp Lys Arg Leu Thr Trp Asp Glu Val Lys Asp Ile           
    375                 380                 385                           

att tca tgg acc aag tta cca gtt ttg ctg aag ggt gtt caa aga act       2215
Ile Ser Trp Thr Lys Leu Pro Val Leu Leu Lys Gly Val Gln Arg Thr           
390                 395                 400                 405           

gat gat att gag aag gca att gat att ggt tgt aag ggt gtt gtc ttg       2263
Asp Asp Ile Glu Lys Ala Ile Asp Ile Gly Cys Lys Gly Val Val Leu           
                410                 415                 420               

tcc aat cat ggt ggt aga caa tta gat act tct cct cct cca ata gaa       2311
Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ser Pro Pro Pro Ile Glu           
            425                 430                 435                   

gtt atg gct gaa tct gtt cca atc cta aag caa aag ggt aaa ctg gat       2359
Val Met Ala Glu Ser Val Pro Ile Leu Lys Gln Lys Gly Lys Leu Asp           
        440                 445                 450                       

cca aat ttc agt att ttc gtt gat ggt ggt gtt aga aga ggt aca gat       2407
Pro Asn Phe Ser Ile Phe Val Asp Gly Gly Val Arg Arg Gly Thr Asp           
    455                 460                 465                           

att ttg aaa gct ttg gct att ggt ggc aga gac tgt aaa gtt gct gtt       2455
Ile Leu Lys Ala Leu Ala Ile Gly Gly Arg Asp Cys Lys Val Ala Val           
470                 475                 480                 485           

ggt ctg ggt aga cct ttc ctt tat gca aat act ggt tat ggt gaa aag       2503
Gly Leu Gly Arg Pro Phe Leu Tyr Ala Asn Thr Gly Tyr Gly Glu Lys           
                490                 495                 500               

ggt gtc aga aag gcc gtg caa att cta aga gaa gaa tta aag gct gat       2551
Gly Val Arg Lys Ala Val Gln Ile Leu Arg Glu Glu Leu Lys Ala Asp           
            505                 510                 515                   

atg aga atg ttg ggc gtt acc tct ttg aac gag cta gac gac tct tac       2599
Met Arg Met Leu Gly Val Thr Ser Leu Asn Glu Leu Asp Asp Ser Tyr           
        520                 525                 530                       

att gac acc aga aga tta cta ggt aga gat gct gtt aac cac ata tac       2647
Ile Asp Thr Arg Arg Leu Leu Gly Arg Asp Ala Val Asn His Ile Tyr           
    535                 540                 545                           

aac aac aac tac tac cca atg tct aag att caa ttc aaa aac gaa aaa       2695
Asn Asn Asn Tyr Tyr Pro Met Ser Lys Ile Gln Phe Lys Asn Glu Lys           
550                 555                 560                 565           

taa gtctgatatt tgctaaattg aaatgaacct taccatgcca catctataga            2748

catcaaaaca ttttcaattt gtcgatatct tttgcatatc aaagtaatac caagcatgtt     2808

caaaaagaaa agaaagcata actttaatac tctattcgaa acattccgat ccacaacaca     2868

ttagtctttt taggcccgtt gttcatcttt ctattacttt attcctaact gtatttttat     2928

aattccgggt ttataaaaga ttaaactaat atagcgcatt ctttttgggt acaaacatac     2988

ataacggagc tcattcatac atcgcttttc agttcgactg gtgtttcgga tgcctctttt     3048

tctaaggagc tagattctgg ccccacacta gtctttgaac tcgttgctcc cttaccaccc     3108

ttaccaccag ccttacttgt aggtttttca gtagcatact ctgcgtgttt gactaaattc     3168

ccttccttaa ctttgtgcca gcttggccat atcattaaat acccactgaa acttctaaca     3228

actcttcgac cttcctgatg ggcctttgaa attgtatcta ccaaacctgc cttcaaggga     3288

tgttctttat atcccctgac gtctttcata ctttgaactt cctctgggac gtcttccttt     3348

ccatattttt cccattggcc cggcttgttt ttagatttgt ctatctcacg gaaaattgag     3408

gggttcatac ttaatccact cacaccaacc ctgatgttag aagacagttt tgctaaatta     3468

tttacattct gacttgtgtt tgtcgatata actgaatcag atggtttcat cgatgattct     3528

cgggagattg gttctgatgg cgtcaccggc gtctcagatg ctgctggatt tagctttagt     3588

ttgagccgtt taataggttc ttcatttaat gtttgttttc tttttttgtc gtagaaatgt     3648

gctgtgagat caggaaattg ttcaagctgt tcacgactca gttttag                   3695


<210>  44
<211>  565
<212>  PRT
<213>  Issatchenkia orientalis

<400>  44

Met Leu Arg Ser Gln Phe Lys Asn Ile Leu Lys Asn Val Asn Lys Asn 
1               5                   10                  15      


His Ser Leu Arg Arg Thr Phe Thr Ser Ser Thr Ser Lys Ala Gly Lys 
            20                  25                  30          


Asn Ala Ser Tyr Asn Ala Lys Ile Ile Ser Ala Thr Val Ala Ser Ile 
        35                  40                  45              


Val Ala Ala Ala Gly Ser Tyr Met Leu Val Gln Pro Ser Leu Ala Asn 
    50                  55                  60                  


Asp Glu Ala Gln Ser Ala Asn Pro Thr Arg Lys Ile Ser Val Asp Glu 
65                  70                  75                  80  


Phe Val Lys His Asn His Ala Asp Asp Cys Trp Ile Thr Val Asn Gly 
                85                  90                  95      


Asn Val Tyr Asp Leu Thr Asp Phe Ile Ser Met His Pro Gly Gly Thr 
            100                 105                 110         


Thr Pro Leu Ile Gln Asn Ala Gly His Asp Ala Thr Glu Ile Tyr Asn 
        115                 120                 125             


Lys Ile His Pro Lys Gly Thr Ile Glu Asn Phe Leu Pro Lys Glu Lys 
    130                 135                 140                 


Gln Leu Gly Val Leu Asp Gly Glu Ala Pro Lys Ile Glu Val Val Leu 
145                 150                 155                 160 


Asp Glu Lys Glu Lys His Arg Leu Glu Leu Leu Asn His Leu Pro Ala 
                165                 170                 175     


Leu Ser Arg Ile Gln Asn Ile Tyr Asp Phe Glu His Ile Ala Ser Arg 
            180                 185                 190         


Val Leu Ser Asp Gln Ala Trp Asn Tyr Tyr Ser Cys Gly Ala Glu Asp 
        195                 200                 205             


Glu Ile Thr Leu Arg Glu Asn His Tyr Ala Tyr Gln Arg Ile Tyr Phe 
    210                 215                 220                 


Lys Pro Lys Cys Cys Val Asn Val Ala Glu Val Asp Thr Ser His Glu 
225                 230                 235                 240 


Ile Leu Gly Thr Lys Ala Ser Val Pro Phe Tyr Val Ser Ala Ala Ala 
                245                 250                 255     


Ser Ala Lys Leu Gly His Glu Asp Gly Glu Cys Ser Ile Ala Arg Gly 
            260                 265                 270         


Ala Gly Lys Glu Gly Val Ile Gln Met Ile Ser Ser Phe Ser Ser Asn 
        275                 280                 285             


Ser Leu Glu Glu Ile Ala Glu Ser Arg Ile Pro Gly Ala Thr Gln Trp 
    290                 295                 300                 


Phe Gln Leu Tyr Val Asn Glu Asp Lys Asp Val Val Lys Lys Thr Leu 
305                 310                 315                 320 


Lys Arg Ala Glu Asn Leu Gly Met Lys Ala Ile Phe Val Thr Val Asp 
                325                 330                 335     


Ala Ala Ser Arg Gly Asn Arg Glu Lys Asp Ile Arg Met Arg Ile Thr 
            340                 345                 350         


Glu Asp Thr Asp Glu Leu Ile Asp Asp Ser Ser Val Arg Ala Gly Ser 
        355                 360                 365             


Thr Ser Gly Ala Leu Pro Ala Phe Ile Asp Lys Arg Leu Thr Trp Asp 
    370                 375                 380                 


Glu Val Lys Asp Ile Ile Ser Trp Thr Lys Leu Pro Val Leu Leu Lys 
385                 390                 395                 400 


Gly Val Gln Arg Thr Asp Asp Ile Glu Lys Ala Ile Asp Ile Gly Cys 
                405                 410                 415     


Lys Gly Val Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ser 
            420                 425                 430         


Pro Pro Pro Ile Glu Val Met Ala Glu Ser Val Pro Ile Leu Lys Gln 
        435                 440                 445             


Lys Gly Lys Leu Asp Pro Asn Phe Ser Ile Phe Val Asp Gly Gly Val 
    450                 455                 460                 


Arg Arg Gly Thr Asp Ile Leu Lys Ala Leu Ala Ile Gly Gly Arg Asp 
465                 470                 475                 480 


Cys Lys Val Ala Val Gly Leu Gly Arg Pro Phe Leu Tyr Ala Asn Thr 
                485                 490                 495     


Gly Tyr Gly Glu Lys Gly Val Arg Lys Ala Val Gln Ile Leu Arg Glu 
            500                 505                 510         


Glu Leu Lys Ala Asp Met Arg Met Leu Gly Val Thr Ser Leu Asn Glu 
        515                 520                 525             


Leu Asp Asp Ser Tyr Ile Asp Thr Arg Arg Leu Leu Gly Arg Asp Ala 
    530                 535                 540                 


Val Asn His Ile Tyr Asn Asn Asn Tyr Tyr Pro Met Ser Lys Ile Gln 
545                 550                 555                 560 


Phe Lys Asn Glu Lys 
                565 


<210>  45
<211>  1960
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (411)..(1343)

<400>  45
gtacgactca tggggcttta caaacacctt acatgttgta tacagtagag cggggggcaa       60

caacctatgg gggtgaatat gaagctctta tgacgagtca ccccgatttc cccgttatcc      120

gacacgagcg atttgaaagc ccgttttctt acctgttagg gaaattgtcc gtgggcatgt      180

ttctccgttc caagggttcc gtttgccgtc aggtatctaa ggatagggca caatagtcag      240

cggggaaacg gagttcccga gggtgtcgga gatactccag ctaattgacc cctagataaa      300

tattcatgat taattcgact attataaggt cattcttccc atgttctttc cattcatcta      360

tataaggtgt ccctggttcc tttattaaga agaaaacaac tgcaacactt atg act         416
                                                       Met Thr            
                                                       1                  

ttg aag tcc aaa tca aaa tcc aaa aag aag cac gca tcc tca aaa cca        464
Leu Lys Ser Lys Ser Lys Ser Lys Lys Lys His Ala Ser Ser Lys Pro           
        5                   10                  15                        

ctg gaa tca tct aat aaa atg tcc aaa agt agt gtt gaa cac cat gaa        512
Leu Glu Ser Ser Asn Lys Met Ser Lys Ser Ser Val Glu His His Glu           
    20                  25                  30                            

cat acc tca aat aag gaa aat gac cat att tcc ttg cat tcc cgc ttg        560
His Thr Ser Asn Lys Glu Asn Asp His Ile Ser Leu His Ser Arg Leu           
35                  40                  45                  50            

acg aat att gaa cat cag atc atg ggc aaa gta cat aca agt gat gac        608
Thr Asn Ile Glu His Gln Ile Met Gly Lys Val His Thr Ser Asp Asp           
                55                  60                  65                

ggc gcc tat gtt atc tta gac aac aaa aag tat cct aag tca gaa tta        656
Gly Ala Tyr Val Ile Leu Asp Asn Lys Lys Tyr Pro Lys Ser Glu Leu           
            70                  75                  80                    

ttg aag gcc ttt ggt ggt ttt atg aac cct ggt tgg gca gtg cct tcc        704
Leu Lys Ala Phe Gly Gly Phe Met Asn Pro Gly Trp Ala Val Pro Ser           
        85                  90                  95                        

gaa cac aag ttt ggt aat cca gct cct ttg ggt cta tct gca ttt gcg        752
Glu His Lys Phe Gly Asn Pro Ala Pro Leu Gly Leu Ser Ala Phe Ala           
    100                 105                 110                           

tat tgt act ttt gtt gca tct ttg gtc aac atg caa act aga cat gtt        800
Tyr Cys Thr Phe Val Ala Ser Leu Val Asn Met Gln Thr Arg His Val           
115                 120                 125                 130           

gaa aat gat gct gtt aat gtc ggt gct gca atg ttt tat ggt ggt ttt        848
Glu Asn Asp Ala Val Asn Val Gly Ala Ala Met Phe Tyr Gly Gly Phe           
                135                 140                 145               

atc cag ttc att gcc gga ctt tgg gaa ata tcg ctt gaa aac gct ttt        896
Ile Gln Phe Ile Ala Gly Leu Trp Glu Ile Ser Leu Glu Asn Ala Phe           
            150                 155                 160                   

ggt ggt ttg gca ttt tgc tct ttt gga ggt tac tgg atg gca tcg gcc        944
Gly Gly Leu Ala Phe Cys Ser Phe Gly Gly Tyr Trp Met Ala Ser Ala           
        165                 170                 175                       

tca aac cat atc ccc tgg ttc cat att gct agc tct tat act aca gaa        992
Ser Asn His Ile Pro Trp Phe His Ile Ala Ser Ser Tyr Thr Thr Glu           
    180                 185                 190                           

gca gaa ttc aaa tca ggt atg gga ttt ttc tac ctt ggt tgg cta ctc       1040
Ala Glu Phe Lys Ser Gly Met Gly Phe Phe Tyr Leu Gly Trp Leu Leu           
195                 200                 205                 210           

ttt aca ata atc ttg cta gct tgt tca atc aaa tct acc att tta ttt       1088
Phe Thr Ile Ile Leu Leu Ala Cys Ser Ile Lys Ser Thr Ile Leu Phe           
                215                 220                 225               

ttc ctg ttg ttt gtg ctg gtc ttt atg aga ttg ctg tta tta aca tgt       1136
Phe Leu Leu Phe Val Leu Val Phe Met Arg Leu Leu Leu Leu Thr Cys           
            230                 235                 240                   

tgg aag ttt gcg gac agt cat gcc tgt gag ttt gct gct ggt gtt ttc       1184
Trp Lys Phe Ala Asp Ser His Ala Cys Glu Phe Ala Ala Gly Val Phe           
        245                 250                 255                       

ggt gtt ttg gca tct ctg tta gca tgg tat cat gca tat gca ggt att       1232
Gly Val Leu Ala Ser Leu Leu Ala Trp Tyr His Ala Tyr Ala Gly Ile           
    260                 265                 270                           

gca aca cct cag aat tct tac tat gtt gtt aat cca aca cct atg cct       1280
Ala Thr Pro Gln Asn Ser Tyr Tyr Val Val Asn Pro Thr Pro Met Pro           
275                 280                 285                 290           

gtt att gga tca aag agc aaa gat atg ttt gat tct gac gac ttt gac       1328
Val Ile Gly Ser Lys Ser Lys Asp Met Phe Asp Ser Asp Asp Phe Asp           
                295                 300                 305               

caa tct tca tct tga tctagagtcc ctctaattct tcctgttgtt tttttctcgg       1383
Gln Ser Ser Ser                                                           
            310                                                           

ttcacttctt ccaccacttt gggtttgaaa cctcctttta taaactgtgt ttttatccat     1443

ttttttttgt tcttactgct tacaacttaa tataatacac tatataacaa aaaattccgt     1503

ttatttctac atcactatgt gacgaattgt atattcatct gttcatcaag gtcccacact     1563

tcttacctga aatgttccac caatattaga aaacctgcac ttatcactct aacttatgta     1623

tagtacttag aggaagttac aattatcagt gtctgtaccg gccacaatgg ttgatatcct     1683

ccaatacgat acagccccac atacttaagt tttcacatat tgcgcataat aaataggtgc     1743

aatctgaccc ttgccaaata attcggtcat tccacgtcta gaccggacaa caagtggaaa     1803

aaatagatct atgaggatct gagcaacgtc aatcaaatcc atattattct cctgcccgat     1863

tttgaccgtt gtgagtccta cagaacacag atggacacac actgagtcat ctccacaact     1923

acacccccat ctcgtataaa tgagtgctcg atcactg                              1960


<210>  46
<211>  310
<212>  PRT
<213>  Issatchenkia orientalis

<400>  46

Met Thr Leu Lys Ser Lys Ser Lys Ser Lys Lys Lys His Ala Ser Ser 
1               5                   10                  15      


Lys Pro Leu Glu Ser Ser Asn Lys Met Ser Lys Ser Ser Val Glu His 
            20                  25                  30          


His Glu His Thr Ser Asn Lys Glu Asn Asp His Ile Ser Leu His Ser 
        35                  40                  45              


Arg Leu Thr Asn Ile Glu His Gln Ile Met Gly Lys Val His Thr Ser 
    50                  55                  60                  


Asp Asp Gly Ala Tyr Val Ile Leu Asp Asn Lys Lys Tyr Pro Lys Ser 
65                  70                  75                  80  


Glu Leu Leu Lys Ala Phe Gly Gly Phe Met Asn Pro Gly Trp Ala Val 
                85                  90                  95      


Pro Ser Glu His Lys Phe Gly Asn Pro Ala Pro Leu Gly Leu Ser Ala 
            100                 105                 110         


Phe Ala Tyr Cys Thr Phe Val Ala Ser Leu Val Asn Met Gln Thr Arg 
        115                 120                 125             


His Val Glu Asn Asp Ala Val Asn Val Gly Ala Ala Met Phe Tyr Gly 
    130                 135                 140                 


Gly Phe Ile Gln Phe Ile Ala Gly Leu Trp Glu Ile Ser Leu Glu Asn 
145                 150                 155                 160 


Ala Phe Gly Gly Leu Ala Phe Cys Ser Phe Gly Gly Tyr Trp Met Ala 
                165                 170                 175     


Ser Ala Ser Asn His Ile Pro Trp Phe His Ile Ala Ser Ser Tyr Thr 
            180                 185                 190         


Thr Glu Ala Glu Phe Lys Ser Gly Met Gly Phe Phe Tyr Leu Gly Trp 
        195                 200                 205             


Leu Leu Phe Thr Ile Ile Leu Leu Ala Cys Ser Ile Lys Ser Thr Ile 
    210                 215                 220                 


Leu Phe Phe Leu Leu Phe Val Leu Val Phe Met Arg Leu Leu Leu Leu 
225                 230                 235                 240 


Thr Cys Trp Lys Phe Ala Asp Ser His Ala Cys Glu Phe Ala Ala Gly 
                245                 250                 255     


Val Phe Gly Val Leu Ala Ser Leu Leu Ala Trp Tyr His Ala Tyr Ala 
            260                 265                 270         


Gly Ile Ala Thr Pro Gln Asn Ser Tyr Tyr Val Val Asn Pro Thr Pro 
        275                 280                 285             


Met Pro Val Ile Gly Ser Lys Ser Lys Asp Met Phe Asp Ser Asp Asp 
    290                 295                 300                 


Phe Asp Gln Ser Ser Ser 
305                 310 


<210>  47
<211>  1032
<212>  DNA
<213>  Bacteriophage P1


<220>
<221>  CDS
<222>  (1)..(1032)

<400>  47
atg tct aat tta ctt act gtt cac caa aac ttg cct gca tta cca gtt         48
Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val           
1               5                   10                  15                

gac gca acc tcc gat gaa gtc aga aag aac ctt atg gat atg ttt aga         96
Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg           
            20                  25                  30                    

gat aga caa gct ttc tcc gaa cat act tgg aaa atg tta tta tcc gtt        144
Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val           
        35                  40                  45                        

tgt aga tcc tgg gcc gct tgg tgt aaa ctt aac aat aga aaa tgg ttt        192
Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe           
    50                  55                  60                            

cct gct gaa cca gaa gac gtc aga gat tac tta ctt tac tta caa gct        240
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala           
65                  70                  75                  80            

aga ggt ttg gct gtt aaa act atc caa caa cac tta ggt caa ttg aat        288
Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn           
                85                  90                  95                

atg tta cac aga aga tcc ggt tta cca aga cca tcc gat tcc aac gca        336
Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala           
            100                 105                 110                   

gtt tcc ctt gtt atg aga aga att aga aaa gaa aat gtt gac gct ggt        384
Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly           
        115                 120                 125                       

gaa aga gct aaa caa gca tta gca ttt gaa aga acc gat ttc gat caa        432
Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln           
    130                 135                 140                           

gtt aga tcc tta atg gaa aat tcc gat aga tgt caa gat att aga aac        480
Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn           
145                 150                 155                 160           

tta gct ttc tta ggt att gct tac aac aca tta tta aga atc gct gaa        528
Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu           
                165                 170                 175               

att gct aga att aga gtt aaa gat att tca aga acc gat ggc ggt aga        576
Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg           
            180                 185                 190                   

atg tta atc cac att ggc aga aca aaa acc tta gtc tcc aca gca ggc        624
Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly           
        195                 200                 205                       

gtc gaa aaa gca tta tca tta ggt gtt act aaa tta gtt gaa cgt tgg        672
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp           
    210                 215                 220                           

att tcc gtt tcc ggt gtt gca gat gac cca aac aac tac tta ttc tgt        720
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys           
225                 230                 235                 240           

cgt gtt aga aaa aat ggt gtt gcc gct cct tcc gct acc tca caa tta        768
Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu           
                245                 250                 255               

tcc aca aga gca tta gaa ggc att ttt gaa gct acc cac aga ctt att        816
Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile           
            260                 265                 270                   

tat ggt gca aaa gac gat tcc ggt caa aga tat tta gct tgg tct ggt        864
Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly           
        275                 280                 285                       

cat tcc gct aga gtt ggt gcc gca aga gac atg gca aga gct ggt gtt        912
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val           
    290                 295                 300                           

tct att cct gaa att atg caa gcc ggt ggt tgg act aat gtt aac att        960
Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile           
305                 310                 315                 320           

gtt atg aac tat atc aga aac tta gat tcc gaa aca ggt gct atg gtt       1008
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val           
                325                 330                 335               

aga tta ctt gaa gac ggt gat taa                                       1032
Arg Leu Leu Glu Asp Gly Asp                                               
            340                                                           


<210>  48
<211>  343
<212>  PRT
<213>  Bacteriophage P1

<400>  48

Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  49
caacggcaac agtttacagg                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  50
tctctggtct tcaaacatgg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  51
ttggcgcttc accatctaac                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  52
actcttctgc tcgttgtgtc                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  53
gtttgaccag acctttagcg                                                   20


<210>  54
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  54
cttcaaagtg gtgcatgcgg tgag                                              24


<210>  55
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  55
gaaaatgcat gcaacggcaa catcaatgtc cacg                                   34


<210>  56
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  56
gaaagtcgac ggtaaggccc gggaattcag cttgc                                  35


<210>  57
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  57
ataattcccg ggaacctcag ggagaacttt gg                                     32


<210>  58
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  58
tataatgaat tcggctactc tatataatat gcttg                                  35


<210>  59
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  59
aaaaaagagc tcacattcgc cacattcgac                                        30


<210>  60
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  60
aaaaaacctg caggtggaag gtgactcaat c                                      31


<210>  61
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  61
aaaaaagcta gccctctttg aacgagctag ac                                     32


<210>  62
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  62
aaaaaagggc ccgtcgtgaa cagcttgaac                                        30


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  63
ggtgcttcaa aggcactatc                                                   20


<210>  64
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  64
gaaaggatcc atgtctaatt tacttactgt tcac                                   34


<210>  65
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  65
gaaattaatt aacttaatca ccgtcttcaa gtaatctaac c                           41


<210>  66
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  66
cacgcgtggc gcgccgcggc cgcatgtcca atgttaaagt agctctac                    48


<210>  67
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  67
ctggcaaacc tgcagggcgg ccgcttaacg taccataaaa ttcag                       45


<210>  68
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  68
cacgcgtggc gcgccgcggc cgcatggtca aggtgactat tttagg                      46


<210>  69
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  69
ctggcaaacc tgcagggcgg ccgcttacag ttttgggcca tggac                       45


<210>  70
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  70
ctggcaaacc tgcagggcgg ccgcttatgg gttagccttg acaaag                      46


<210>  71
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  71
cacgcgtggc gcgccgcggc cgcatgtcgc aaagaaaatt c                           41


<210>  72
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  72
ctggcaaacc tgcagggcgg ccgcttatgc cttagtttca acaggaac                    48


<210>  73
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  73
gtttaaacct actatgtaca ctgtataagt aaaaag                                 36


<210>  74
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  74
gagctcagcg gccgctcata tgggcgtgtt ctttttaatt attgctc                     47


<210>  75
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  75
catatgagcg gccgctgagc tctggacaca tacacattat caaatg                      46


<210>  76
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  76
gtttaaacct ttcaaaacca aagttggtaa acatac                                 36


<210>  77
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  77
gtttaaacgt acgactcatg gggctttaca aac                                    33


<210>  78
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  78
gagctcagcg gccgctcata tgaagtgttg cagttgtttt cttcttaata aag              53


<210>  79
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  79
catatgagcg gccgctgagc tctctagagt ccctctaatt cttcctgttg                  50


<210>  80
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  80
gtttaaacca gtgatcgagc actcatttat acgagatg                               38


<210>  81
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  81
gtttaaacca ttttaatttc tattgctata atgtc                                  35


<210>  82
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  82
gagctcagcg gccgctcata tgatttttgt gttttgctgt gttttg                      46


<210>  83
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  83
catatgagcg gccgctgagc tctgacatct gaatgtaaaa tgaac                       45


<210>  84
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  84
gtttaaacgt caaggttttg gcaagctgcc tgtttg                                 36


<210>  85
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  85
gtttaaacga aactaagaca taacaaaaaa gcatgggatg                             40


<210>  86
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  86
gagctcagcg gccgctcata tgttttttaa agcaatttaa atgaatatta tttg             54


<210>  87
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  87
catatgagcg gccgctgagc tcctttataa atggtttcat atcaatatta tgactc           56


<210>  88
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  88
gtttaaacca aatggaacaa tgagagatgg tacgcggaac                             40


<210>  89
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  89
ttaatcggcc aaccatacgg tgggttccca gaacc                                  35


<210>  90
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  90
ggttctggga acccaccgta tggttggccg attaa                                  35


<210>  91
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  91
cttctcaaaa cagagcacaa aaactattgc ac                                     32


<210>  92
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  92
gtgcaatagt ttttgtgctc tgttttgaga ag                                     32


<210>  93
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  93
cacgcgtggc gcgccgcggc catgtctacc caaaacgatc tg                          42


<210>  94
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  94
ctggcaaacc tgcagggcgg ccttattctt tagctggagc ttcttc                      46


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  95
cagagagagg aagaagttgg aac                                               23


<210>  96
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  96
gtacagagaa cttgtaaaca attc                                              24


<210>  97
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  97
taatgggtac accatacggt ggattcccag ag                                     32


<210>  98
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  98
ctctgggaat ccaccgtatg gtgtacccat ta                                     32


<210>  99
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  99
cacgcgtggc gcgccgcggc catgcttaga gccctaactc                             40


<210>  100
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  100
ctggcaaacc tgcagggcgg ccttagtttt gcttgacaaa g                           41


<210>  101
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  101
cacgcgtggc gcgccgcggc catggttagc gttgcag                                37


<210>  102
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  102
ctggcaaacc tgcagggcgg ccttacaact ttgtctgctg                             40


<210>  103
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  103
cacgcgtggc gcgccgcggc catgccagca gtatcatatg atg                         43


<210>  104
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  104
ctggcaaacc tgcagggcgg ccttatacca agcttagacc cttg                        44


<210>  105
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  105
cacgcgtggc gcgccgcggc cgcatgttct ccagaatctc tgctag                      46


<210>  106
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  106
gtttaaacct cggtagtgcc taccaattta atg                                    33


<210>  107
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  107
gtttaaacct ccgttatgta tgtttgtac                                         29


<210>  108
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  108
cacttgactt cgtcaatgaa tac                                               23


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  109
gtctgatcta aatggttctg                                                   20


<210>  110
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  110
gtctcagaga cagagaactc tatgacgcgt acg                                    33


<210>  111
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  111
cgtacgcgtc atagagttct ctgtctctga gac                                    33


<210>  112
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  112
taccgggcgg gaaggaactc cagcagaagt tgg                                    33


<210>  113
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  113
ccaacttctg ctggagttcc ttcccgcccg gta                                    33


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  114
ggagtgacac aacctgaaag                                                   20


<210>  115
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  115
aatattctat tattatatat tttcttccca ataaaacaaa ataaaacaaa acacagcaaa       60

acacaaaaat cctggaattc gcccttacat atgg                                   94


<210>  116
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  116
tatagattgt aaagtagacg taaagtttag taattcattt taatgttcat tttacattca       60

gatgtcatta cggctcgtgc tatattcttg                                        90


<210>  117
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  117
aaaaaaacgc gtatgtcaac tgtggaagat cac                                    33


<210>  118
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  118
aaaaaacctg caggttaagc tgctggcgct tcatctttgg                             40


<210>  119
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  119
aaaggctgac ggacacaatc                                                   20


<210>  120
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  120
attctattat tatatatttt cttcccaata aaacaaaata aaacaaaaca cagcaaaaca       60

caaaaatcct ggaattcgcc cttacatatg                                        90


<210>  121
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  121
gcgcatccat attttggcgg                                                   20


<210>  122
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  122
atattagtcg acccttctat cagggaaggg ag                                     32


<210>  123
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  123
gtgggctaca aatgatacga tgg                                               23


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  124
tcggccactt gtttattggg                                                   20


<210>  125
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  125
ctgcaggttt gccagcttac                                                   20


<210>  126
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  126
gattgtgtat tagtgtattt cg                                                22


<210>  127
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  127
catgcatgca tgtctagata aaatgttagc tgctagatc                              39


<210>  128
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  128
catgcatgca tgttaattaa cttaatcctt tggaccaatc atg                         43


<210>  129
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  129
catcactgtt aaaggaatgg gtaaatc                                           27


<210>  130
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  130
cgaaaccgtt aaattcaaca atg                                               23


<210>  131
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  131
gcatctaatg tacgttccaa c                                                 21


<210>  132
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  132
cttctatagg ttgagaccc                                                    19


<210>  133
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  133
gagtgacaca acctgaaag                                                    19


<210>  134
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  134
gtaaaacgac ggccag                                                       16


<210>  135
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  135
caggaaacag ctatgac                                                      17


<210>  136
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  136
caagagtatc ccatctgaca ggaaccgatg g                                      31


<210>  137
<211>  1071
<212>  DNA
<213>  Zygosaccharomyces rouxii


<220>
<221>  CDS
<222>  (1)..(1071)

<400>  137
atg cct cat tct atc aac ggt gat gtt aaa atc gca gta ttg gga gct         48
Met Pro His Ser Ile Asn Gly Asp Val Lys Ile Ala Val Leu Gly Ala           
1               5                   10                  15                

gca ggt ggt att gga caa tca ctt tcg cta ctt ttg aag acc cag tta         96
Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu Leu Lys Thr Gln Leu           
            20                  25                  30                    

act aga gaa ttg cca aat cat cgt cat gct cag tta gcc cta tac gac        144
Thr Arg Glu Leu Pro Asn His Arg His Ala Gln Leu Ala Leu Tyr Asp           
        35                  40                  45                        

gtc aat gct gac gca gtt cgg ggt gtc gca gcc gac tta tct cat att        192
Val Asn Ala Asp Ala Val Arg Gly Val Ala Ala Asp Leu Ser His Ile           
    50                  55                  60                            

gat aca ggt gtt act gta aca gga tat gaa ggt gat agg atc ggc gaa        240
Asp Thr Gly Val Thr Val Thr Gly Tyr Glu Gly Asp Arg Ile Gly Glu           
65                  70                  75                  80            

gcg tta gaa ggt acg gat atc gtc ctg atc cct gca ggt gtt cct aga        288
Ala Leu Glu Gly Thr Asp Ile Val Leu Ile Pro Ala Gly Val Pro Arg           
                85                  90                  95                

aaa cct ggt atg aca aga gaa gat cta ttg gtt gtt aat gca aag att        336
Lys Pro Gly Met Thr Arg Glu Asp Leu Leu Val Val Asn Ala Lys Ile           
            100                 105                 110                   

gtc aag agt ata ggg tca tcg att gcg cag cat tgc gat tta aac aaa        384
Val Lys Ser Ile Gly Ser Ser Ile Ala Gln His Cys Asp Leu Asn Lys           
        115                 120                 125                       

gtg ttc att cta cta atc tca aac cca ata aat tcc ctt gtt cca gta        432
Val Phe Ile Leu Leu Ile Ser Asn Pro Ile Asn Ser Leu Val Pro Val           
    130                 135                 140                           

ctc gtt aag gaa ctg gaa tct aaa tct caa ggc act caa gtt gag aga        480
Leu Val Lys Glu Leu Glu Ser Lys Ser Gln Gly Thr Gln Val Glu Arg           
145                 150                 155                 160           

cgt gtg ctt ggt ctc act aag ttg gat tcc gtt aga gca agt gca ttt        528
Arg Val Leu Gly Leu Thr Lys Leu Asp Ser Val Arg Ala Ser Ala Phe           
                165                 170                 175               

ttg cac gag gtt acg att aaa cat ggt cta aaa cct aaa tct aat act        576
Leu His Glu Val Thr Ile Lys His Gly Leu Lys Pro Lys Ser Asn Thr           
            180                 185                 190                   

ctt gat gat gtt cca gta gtt ggt ggt cat tct ggt gaa act att gta        624
Leu Asp Asp Val Pro Val Val Gly Gly His Ser Gly Glu Thr Ile Val           
        195                 200                 205                       

cct tta ttc tcc caa gcc cct aat ggt aac cgt tta tca cag gac gcc        672
Pro Leu Phe Ser Gln Ala Pro Asn Gly Asn Arg Leu Ser Gln Asp Ala           
    210                 215                 220                           

ttg gaa gct ctt gtt cag cgt gta caa ttc gga ggc gat gaa gtc gtt        720
Leu Glu Ala Leu Val Gln Arg Val Gln Phe Gly Gly Asp Glu Val Val           
225                 230                 235                 240           

aga gct aaa aat ggt gct ggt agt gcc act ctg tgt atg gcc cat gcc        768
Arg Ala Lys Asn Gly Ala Gly Ser Ala Thr Leu Cys Met Ala His Ala           
                245                 250                 255               

gct tat act gtt gct gca tct ttt att cca ctt atc act ggt caa aag        816
Ala Tyr Thr Val Ala Ala Ser Phe Ile Pro Leu Ile Thr Gly Gln Lys           
            260                 265                 270                   

cgt tcc atc tct ggt aca ttc tat gtt gcc tta aag gat gct caa ggt        864
Arg Ser Ile Ser Gly Thr Phe Tyr Val Ala Leu Lys Asp Ala Gln Gly           
        275                 280                 285                       

cag cct atc aac agt agc gct aag cgt ctt ttg ggc tca atc aac gat        912
Gln Pro Ile Asn Ser Ser Ala Lys Arg Leu Leu Gly Ser Ile Asn Asp           
    290                 295                 300                           

tta cca tat ttt gca gtg cca ttg gag att act tct cag ggt gtg gat        960
Leu Pro Tyr Phe Ala Val Pro Leu Glu Ile Thr Ser Gln Gly Val Asp           
305                 310                 315                 320           

gaa tta gat acc agc gtt ttg gaa aga atg acc aag tat gag aga gaa       1008
Glu Leu Asp Thr Ser Val Leu Glu Arg Met Thr Lys Tyr Glu Arg Glu           
                325                 330                 335               

aga ctc tta gct cct tgt ctg ggt aaa ttg gaa ggt ggt atc aga aac       1056
Arg Leu Leu Ala Pro Cys Leu Gly Lys Leu Glu Gly Gly Ile Arg Asn           
            340                 345                 350                   

ggt ttg agt ttg tga                                                   1071
Gly Leu Ser Leu                                                           
        355                                                               


<210>  138
<211>  356
<212>  PRT
<213>  Zygosaccharomyces rouxii

<400>  138

Met Pro His Ser Ile Asn Gly Asp Val Lys Ile Ala Val Leu Gly Ala 
1               5                   10                  15      


Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu Leu Lys Thr Gln Leu 
            20                  25                  30          


Thr Arg Glu Leu Pro Asn His Arg His Ala Gln Leu Ala Leu Tyr Asp 
        35                  40                  45              


Val Asn Ala Asp Ala Val Arg Gly Val Ala Ala Asp Leu Ser His Ile 
    50                  55                  60                  


Asp Thr Gly Val Thr Val Thr Gly Tyr Glu Gly Asp Arg Ile Gly Glu 
65                  70                  75                  80  


Ala Leu Glu Gly Thr Asp Ile Val Leu Ile Pro Ala Gly Val Pro Arg 
                85                  90                  95      


Lys Pro Gly Met Thr Arg Glu Asp Leu Leu Val Val Asn Ala Lys Ile 
            100                 105                 110         


Val Lys Ser Ile Gly Ser Ser Ile Ala Gln His Cys Asp Leu Asn Lys 
        115                 120                 125             


Val Phe Ile Leu Leu Ile Ser Asn Pro Ile Asn Ser Leu Val Pro Val 
    130                 135                 140                 


Leu Val Lys Glu Leu Glu Ser Lys Ser Gln Gly Thr Gln Val Glu Arg 
145                 150                 155                 160 


Arg Val Leu Gly Leu Thr Lys Leu Asp Ser Val Arg Ala Ser Ala Phe 
                165                 170                 175     


Leu His Glu Val Thr Ile Lys His Gly Leu Lys Pro Lys Ser Asn Thr 
            180                 185                 190         


Leu Asp Asp Val Pro Val Val Gly Gly His Ser Gly Glu Thr Ile Val 
        195                 200                 205             


Pro Leu Phe Ser Gln Ala Pro Asn Gly Asn Arg Leu Ser Gln Asp Ala 
    210                 215                 220                 


Leu Glu Ala Leu Val Gln Arg Val Gln Phe Gly Gly Asp Glu Val Val 
225                 230                 235                 240 


Arg Ala Lys Asn Gly Ala Gly Ser Ala Thr Leu Cys Met Ala His Ala 
                245                 250                 255     


Ala Tyr Thr Val Ala Ala Ser Phe Ile Pro Leu Ile Thr Gly Gln Lys 
            260                 265                 270         


Arg Ser Ile Ser Gly Thr Phe Tyr Val Ala Leu Lys Asp Ala Gln Gly 
        275                 280                 285             


Gln Pro Ile Asn Ser Ser Ala Lys Arg Leu Leu Gly Ser Ile Asn Asp 
    290                 295                 300                 


Leu Pro Tyr Phe Ala Val Pro Leu Glu Ile Thr Ser Gln Gly Val Asp 
305                 310                 315                 320 


Glu Leu Asp Thr Ser Val Leu Glu Arg Met Thr Lys Tyr Glu Arg Glu 
                325                 330                 335     


Arg Leu Leu Ala Pro Cys Leu Gly Lys Leu Glu Gly Gly Ile Arg Asn 
            340                 345                 350         


Gly Leu Ser Leu 
        355     


<210>  139
<211>  939
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(939)

<400>  139
atg aaa gtc gca gtc ctc ggc gct gct ggc ggt att ggc cag gcg ctt         48
Met Lys Val Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ala Leu           
1               5                   10                  15                

gca cta ctg tta aaa acc caa ctg cct tca ggt tca gaa ctc tct ctg         96
Ala Leu Leu Leu Lys Thr Gln Leu Pro Ser Gly Ser Glu Leu Ser Leu           
            20                  25                  30                    

tat gat atc gct cca gtg act ccc ggt gtg gct gtc gat ctg agc cat        144
Tyr Asp Ile Ala Pro Val Thr Pro Gly Val Ala Val Asp Leu Ser His           
        35                  40                  45                        

atc cct act gct gtg aaa atc aaa ggt ttt tct ggt gaa gat gcg act        192
Ile Pro Thr Ala Val Lys Ile Lys Gly Phe Ser Gly Glu Asp Ala Thr           
    50                  55                  60                            

ccg gcg ctg gaa ggc gca gat gtc gtt ctt atc tct gca ggc gta gcg        240
Pro Ala Leu Glu Gly Ala Asp Val Val Leu Ile Ser Ala Gly Val Ala           
65                  70                  75                  80            

cgt aaa ccg ggt atg gat cgt tcc gac ctg ttt aac gtt aac gcc ggc        288
Arg Lys Pro Gly Met Asp Arg Ser Asp Leu Phe Asn Val Asn Ala Gly           
                85                  90                  95                

atc gtg aaa aac ctg gta cag caa gtt gcg aaa acc tgc ccg aaa gcg        336
Ile Val Lys Asn Leu Val Gln Gln Val Ala Lys Thr Cys Pro Lys Ala           
            100                 105                 110                   

tgc att ggt att atc act aac ccg gtt aac acc aca gtt gca att gct        384
Cys Ile Gly Ile Ile Thr Asn Pro Val Asn Thr Thr Val Ala Ile Ala           
        115                 120                 125                       

gct gaa gtg ctg aaa aaa gcc ggt gtt tat gac aaa aac aaa ctg ttc        432
Ala Glu Val Leu Lys Lys Ala Gly Val Tyr Asp Lys Asn Lys Leu Phe           
    130                 135                 140                           

ggc gtt acc acg ctg gat atc att cgt tcc aac acc ttt gtt gcg gaa        480
Gly Val Thr Thr Leu Asp Ile Ile Arg Ser Asn Thr Phe Val Ala Glu           
145                 150                 155                 160           

ctg aaa ggc aaa cag cca ggc gaa gtt gaa gtg ccg gtt att ggc ggt        528
Leu Lys Gly Lys Gln Pro Gly Glu Val Glu Val Pro Val Ile Gly Gly           
                165                 170                 175               

cac tct ggt gtt acc att ctg ccg ctg ctg tca cag gtt cct ggc gtt        576
His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Val           
            180                 185                 190                   

agt ttt acc gag cag gaa gtg gct gat ctg acc aaa cgc atc cag aac        624
Ser Phe Thr Glu Gln Glu Val Ala Asp Leu Thr Lys Arg Ile Gln Asn           
        195                 200                 205                       

gcg ggt act gaa gtg gtt gaa gcg aag gcc ggt ggc ggg tct gca acc        672
Ala Gly Thr Glu Val Val Glu Ala Lys Ala Gly Gly Gly Ser Ala Thr           
    210                 215                 220                           

ctg tct atg ggc cag gca gct gca cgt ttt ggt ctg tct ctg gtt cgt        720
Leu Ser Met Gly Gln Ala Ala Ala Arg Phe Gly Leu Ser Leu Val Arg           
225                 230                 235                 240           

gca ctg cag ggc gaa caa ggc gtt gtc gaa tgt gcc tac gtt gaa ggc        768
Ala Leu Gln Gly Glu Gln Gly Val Val Glu Cys Ala Tyr Val Glu Gly           
                245                 250                 255               

gac ggt cag tac gcc cgt ttc ttc tct caa ccg ctg ctg ctg ggt aaa        816
Asp Gly Gln Tyr Ala Arg Phe Phe Ser Gln Pro Leu Leu Leu Gly Lys           
            260                 265                 270                   

aac ggc gtg gaa gag cgt aaa tct atc ggt acc ctg agc gca ttt gaa        864
Asn Gly Val Glu Glu Arg Lys Ser Ile Gly Thr Leu Ser Ala Phe Glu           
        275                 280                 285                       

cag aac gcg ctg gaa ggt atg ctg gat acg ctg aag aaa gat atc gcc        912
Gln Asn Ala Leu Glu Gly Met Leu Asp Thr Leu Lys Lys Asp Ile Ala           
    290                 295                 300                           

ctg ggc gaa gag ttc gtt aat aag taa                                    939
Leu Gly Glu Glu Phe Val Asn Lys                                           
305                 310                                                   


<210>  140
<211>  312
<212>  PRT
<213>  Escherichia coli

<400>  140

Met Lys Val Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ala Leu 
1               5                   10                  15      


Ala Leu Leu Leu Lys Thr Gln Leu Pro Ser Gly Ser Glu Leu Ser Leu 
            20                  25                  30          


Tyr Asp Ile Ala Pro Val Thr Pro Gly Val Ala Val Asp Leu Ser His 
        35                  40                  45              


Ile Pro Thr Ala Val Lys Ile Lys Gly Phe Ser Gly Glu Asp Ala Thr 
    50                  55                  60                  


Pro Ala Leu Glu Gly Ala Asp Val Val Leu Ile Ser Ala Gly Val Ala 
65                  70                  75                  80  


Arg Lys Pro Gly Met Asp Arg Ser Asp Leu Phe Asn Val Asn Ala Gly 
                85                  90                  95      


Ile Val Lys Asn Leu Val Gln Gln Val Ala Lys Thr Cys Pro Lys Ala 
            100                 105                 110         


Cys Ile Gly Ile Ile Thr Asn Pro Val Asn Thr Thr Val Ala Ile Ala 
        115                 120                 125             


Ala Glu Val Leu Lys Lys Ala Gly Val Tyr Asp Lys Asn Lys Leu Phe 
    130                 135                 140                 


Gly Val Thr Thr Leu Asp Ile Ile Arg Ser Asn Thr Phe Val Ala Glu 
145                 150                 155                 160 


Leu Lys Gly Lys Gln Pro Gly Glu Val Glu Val Pro Val Ile Gly Gly 
                165                 170                 175     


His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Val 
            180                 185                 190         


Ser Phe Thr Glu Gln Glu Val Ala Asp Leu Thr Lys Arg Ile Gln Asn 
        195                 200                 205             


Ala Gly Thr Glu Val Val Glu Ala Lys Ala Gly Gly Gly Ser Ala Thr 
    210                 215                 220                 


Leu Ser Met Gly Gln Ala Ala Ala Arg Phe Gly Leu Ser Leu Val Arg 
225                 230                 235                 240 


Ala Leu Gln Gly Glu Gln Gly Val Val Glu Cys Ala Tyr Val Glu Gly 
                245                 250                 255     


Asp Gly Gln Tyr Ala Arg Phe Phe Ser Gln Pro Leu Leu Leu Gly Lys 
            260                 265                 270         


Asn Gly Val Glu Glu Arg Lys Ser Ile Gly Thr Leu Ser Ala Phe Glu 
        275                 280                 285             


Gln Asn Ala Leu Glu Gly Met Leu Asp Thr Leu Lys Lys Asp Ile Ala 
    290                 295                 300                 


Leu Gly Glu Glu Phe Val Asn Lys 
305                 310         


<210>  141
<211>  987
<212>  DNA
<213>  Rhizopus oryzae


<220>
<221>  CDS
<222>  (1)..(987)

<400>  141
atg gtt aaa gtt aca gtt tgt ggt gct gct ggt ggt att ggt caa ccc         48
Met Val Lys Val Thr Val Cys Gly Ala Ala Gly Gly Ile Gly Gln Pro           
1               5                   10                  15                

ctt tct tta ctc ttg aag caa tcc tct cac att act cac tta tct ctt         96
Leu Ser Leu Leu Leu Lys Gln Ser Ser His Ile Thr His Leu Ser Leu           
            20                  25                  30                    

tat gat atc gtt aat act cct ggt gtt gct gct gat ctt agt cat atc        144
Tyr Asp Ile Val Asn Thr Pro Gly Val Ala Ala Asp Leu Ser His Ile           
        35                  40                  45                        

gat acc aaa tcc aag gtc act ggt cat gta ggt gct gct caa ctt gaa        192
Asp Thr Lys Ser Lys Val Thr Gly His Val Gly Ala Ala Gln Leu Glu           
    50                  55                  60                            

gaa gct atc aag gat tct gat gtt gtc gtt att ccc gct ggt gtc cca        240
Glu Ala Ile Lys Asp Ser Asp Val Val Val Ile Pro Ala Gly Val Pro           
65                  70                  75                  80            

aga aag cca ggt atg acg cgt gat gat ctt ttc aag att aat gct ggt        288
Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Lys Ile Asn Ala Gly           
                85                  90                  95                

att gta cgt gat ttg gct aca gct gct gca aag tac gct cca aag gcc        336
Ile Val Arg Asp Leu Ala Thr Ala Ala Ala Lys Tyr Ala Pro Lys Ala           
            100                 105                 110                   

ttc atg tgt atc att tct aac cca gtc aac tcg act gtc cca atc gtt        384
Phe Met Cys Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile Val           
        115                 120                 125                       

act gaa gta ttc aaa cag cac aat gtt tat gac ccc aaa aga atc ttt        432
Thr Glu Val Phe Lys Gln His Asn Val Tyr Asp Pro Lys Arg Ile Phe           
    130                 135                 140                           

ggt gtt aca aca ctt gat att gtt cgt gca tcc acc ttt gta tcc gaa        480
Gly Val Thr Thr Leu Asp Ile Val Arg Ala Ser Thr Phe Val Ser Glu           
145                 150                 155                 160           

ttg att gga ggt gaa cct aat tca ctt cgt gtt ccc gtc att ggt ggt        528
Leu Ile Gly Gly Glu Pro Asn Ser Leu Arg Val Pro Val Ile Gly Gly           
                165                 170                 175               

cac agc ggc gta acc atc tta cct tta ctc tca cag gtc ccc ggc att        576
His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Ile           
            180                 185                 190                   

gaa aag tta aac caa gaa caa att gag aag gta act cat cgt att caa        624
Glu Lys Leu Asn Gln Glu Gln Ile Glu Lys Val Thr His Arg Ile Gln           
        195                 200                 205                       

ttt ggt ggc gat gaa gtt gtc aag gcc aag gat ggt gct ggt tct gcc        672
Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala           
    210                 215                 220                           

act ctt tcc atg gct tat gct ggt gct cgt ttt gct aca aac atc att        720
Thr Leu Ser Met Ala Tyr Ala Gly Ala Arg Phe Ala Thr Asn Ile Ile           
225                 230                 235                 240           

gag gct gct ttt gct gga aag aag ggc att gtt gaa tgt acc tat gtt        768
Glu Ala Ala Phe Ala Gly Lys Lys Gly Ile Val Glu Cys Thr Tyr Val           
                245                 250                 255               

caa ttg gat gct gat aaa tct ggt gcc caa tct gtc aag gat ttg gtt        816
Gln Leu Asp Ala Asp Lys Ser Gly Ala Gln Ser Val Lys Asp Leu Val           
            260                 265                 270                   

ggt agt gaa ctt gaa tat ttc tct gtt ccc gtt gaa ttg ggt cct agt        864
Gly Ser Glu Leu Glu Tyr Phe Ser Val Pro Val Glu Leu Gly Pro Ser           
        275                 280                 285                       

ggt gtt gaa aag att tta ccc att gga aac gtt aat gaa tat gaa aag        912
Gly Val Glu Lys Ile Leu Pro Ile Gly Asn Val Asn Glu Tyr Glu Lys           
    290                 295                 300                           

aag ttg ttg aac gag gct tct cct gaa tta aaa acc aac att gat aaa        960
Lys Leu Leu Asn Glu Ala Ser Pro Glu Leu Lys Thr Asn Ile Asp Lys           
305                 310                 315                 320           

ggt tgt act ttt gtt act gaa ggc taa                                    987
Gly Cys Thr Phe Val Thr Glu Gly                                           
                325                                                       


<210>  142
<211>  328
<212>  PRT
<213>  Rhizopus oryzae

<400>  142

Met Val Lys Val Thr Val Cys Gly Ala Ala Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Gln Ser Ser His Ile Thr His Leu Ser Leu 
            20                  25                  30          


Tyr Asp Ile Val Asn Thr Pro Gly Val Ala Ala Asp Leu Ser His Ile 
        35                  40                  45              


Asp Thr Lys Ser Lys Val Thr Gly His Val Gly Ala Ala Gln Leu Glu 
    50                  55                  60                  


Glu Ala Ile Lys Asp Ser Asp Val Val Val Ile Pro Ala Gly Val Pro 
65                  70                  75                  80  


Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Lys Ile Asn Ala Gly 
                85                  90                  95      


Ile Val Arg Asp Leu Ala Thr Ala Ala Ala Lys Tyr Ala Pro Lys Ala 
            100                 105                 110         


Phe Met Cys Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile Val 
        115                 120                 125             


Thr Glu Val Phe Lys Gln His Asn Val Tyr Asp Pro Lys Arg Ile Phe 
    130                 135                 140                 


Gly Val Thr Thr Leu Asp Ile Val Arg Ala Ser Thr Phe Val Ser Glu 
145                 150                 155                 160 


Leu Ile Gly Gly Glu Pro Asn Ser Leu Arg Val Pro Val Ile Gly Gly 
                165                 170                 175     


His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Ile 
            180                 185                 190         


Glu Lys Leu Asn Gln Glu Gln Ile Glu Lys Val Thr His Arg Ile Gln 
        195                 200                 205             


Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala 
    210                 215                 220                 


Thr Leu Ser Met Ala Tyr Ala Gly Ala Arg Phe Ala Thr Asn Ile Ile 
225                 230                 235                 240 


Glu Ala Ala Phe Ala Gly Lys Lys Gly Ile Val Glu Cys Thr Tyr Val 
                245                 250                 255     


Gln Leu Asp Ala Asp Lys Ser Gly Ala Gln Ser Val Lys Asp Leu Val 
            260                 265                 270         


Gly Ser Glu Leu Glu Tyr Phe Ser Val Pro Val Glu Leu Gly Pro Ser 
        275                 280                 285             


Gly Val Glu Lys Ile Leu Pro Ile Gly Asn Val Asn Glu Tyr Glu Lys 
    290                 295                 300                 


Lys Leu Leu Asn Glu Ala Ser Pro Glu Leu Lys Thr Asn Ile Asp Lys 
305                 310                 315                 320 


Gly Cys Thr Phe Val Thr Glu Gly 
                325             


