                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH
       F. Hoffmann-La Roche AG
 
<120>  A diagnostic method for type II diabetis

<130>  27327 WO-TH

<150>  EP11155259.2
<151>  2011-02-21

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  222
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Leu Trp Leu Leu Phe Phe Leu Val Thr Ala Ile His Ala Glu Leu 
1               5                   10                  15      


Cys Gln Pro Gly Ala Glu Asn Ala Phe Lys Val Arg Leu Ser Ile Arg 
            20                  25                  30          


Thr Ala Leu Gly Asp Lys Ala Tyr Ala Trp Asp Thr Asn Glu Glu Tyr 
        35                  40                  45              


Leu Phe Lys Ala Met Val Ala Phe Ser Met Arg Lys Val Pro Asn Arg 
    50                  55                  60                  


Glu Ala Thr Glu Ile Ser His Val Leu Leu Cys Asn Val Thr Gln Arg 
65                  70                  75                  80  


Val Ser Phe Trp Phe Val Val Thr Asp Pro Ser Lys Asn His Thr Leu 
                85                  90                  95      


Pro Ala Val Glu Val Gln Ser Ala Ile Arg Met Asn Lys Asn Arg Ile 
            100                 105                 110         


Asn Asn Ala Phe Phe Leu Asn Asp Gln Thr Leu Glu Phe Leu Lys Ile 
        115                 120                 125             


Pro Ser Thr Leu Ala Pro Pro Met Asp Pro Ser Val Pro Ile Trp Ile 
    130                 135                 140                 


Ile Ile Phe Gly Val Ile Phe Cys Ile Ile Ile Val Ala Ile Ala Leu 
145                 150                 155                 160 


Leu Ile Leu Ser Gly Ile Trp Gln Arg Arg Arg Lys Asn Lys Glu Pro 
                165                 170                 175     


Ser Glu Val Asp Asp Ala Glu Asp Lys Cys Glu Asn Met Ile Thr Ile 
            180                 185                 190         


Glu Asn Gly Ile Pro Ser Asp Pro Leu Asp Met Lys Gly Gly His Ile 
        195                 200                 205             


Asn Asp Ala Phe Met Thr Glu Asp Glu Arg Leu Thr Pro Leu 
    210                 215                 220         


<210>  2
<211>  496
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression construct TMEM27(125)-His8

<400>  2
gtcgaccatg ttgtggctgc tcttttttct ggtgactgcc attcatgctg aactctgtca       60

accaggtgca gaaaatgctt ttaaagtgag acttagtatc agaacagctc tgggagataa      120

agcatatgcc tgggatacca atgaagaata cctcttcaaa gcgatggtag ctttctccat      180

gagaaaagtt cccaacagag aagcaacaga aatttcccat gtcctacttt gcaatgtaac      240

ccagagggta tcattctggt ttgtggttac agacccttca aaaaatcaca cccttcctgc      300

tgttgaggtg caatcagcca taagaatgaa caagaaccgg atcaacaatg ccttctttct      360

aaatgaccaa actctggaat ttgcggccgc tggaggtggc agcggcctga acgatatttt      420

tgaagcgcag aaaatcgaat ggcacgaagg aggtggcagc catcaccacc atcaccacca      480

tcactaataa ggatcc                                                      496


<210>  3
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Deduced protein sequence from expression construct 
       TMEM27(125)-His8

<400>  3

Met Leu Trp Leu Leu Phe Phe Leu Val Thr Ala Ile His Ala Glu Leu 
1               5                   10                  15      


Cys Gln Pro Gly Ala Glu Asn Ala Phe Lys Val Arg Leu Ser Ile Arg 
            20                  25                  30          


Thr Ala Leu Gly Asp Lys Ala Tyr Ala Trp Asp Thr Asn Glu Glu Tyr 
        35                  40                  45              


Leu Phe Lys Ala Met Val Ala Phe Ser Met Arg Lys Val Pro Asn Arg 
    50                  55                  60                  


Glu Ala Thr Glu Ile Ser His Val Leu Leu Cys Asn Val Thr Gln Arg 
65                  70                  75                  80  


Val Ser Phe Trp Phe Val Val Thr Asp Pro Ser Lys Asn His Thr Leu 
                85                  90                  95      


Pro Ala Val Glu Val Gln Ser Ala Ile Arg Met Asn Lys Asn Arg Ile 
            100                 105                 110         


Asn Asn Ala Phe Phe Leu Asn Asp Gln Thr Leu Glu Phe Ala Ala Ala 
        115                 120                 125             


Gly Gly Gly Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu 
    130                 135                 140                 


Trp His Glu Gly Gly Gly Ser His His His His His His His His 
145                 150                 155                 


<210>  4
<211>  1006
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression construct TMEM27(125)-sCD40L

<400>  4
gtcgaccatg ttgtggctgc tcttttttct ggtgactgcc attcatgctg aactctgtca       60

accaggtgca gaaaatgctt ttaaagtgag acttagtatc agaacagctc tgggagataa      120

agcatatgcc tgggatacca atgaagaata cctcttcaaa gcgatggtag ctttctccat      180

gagaaaagtt cccaacagag aagcaacaga aatttcccat gtcctacttt gcaatgtaac      240

ccagagggta tcattctggt ttgtggttac agacccttca aaaaatcaca cccttcctgc      300

tgttgaggtg caatcagcca taagaatgaa caagaaccgg atcaacaatg ccttctttct      360

aaatgaccaa actctggaat ttgcggccgc ttccggcgga ggcagcggcg gaggctccgg      420

cggaggcagc ggcggaggct ccggcggagg cagcggcgga ggcatgcaaa gaggtgatga      480

ggaccctcaa attgcagcac acgttgtaag cgaagccaac agtaatgcag catccgttct      540

acagtgggcc aagaaaggat attataccat gaaaagcaac ttggtaatgc ttgaaaatgg      600

gaaacagctg acggttaaaa gagaaggact ctattatgtc tacactcaag tcaccttctg      660

ctctaatcgg gagccttcga gtcaacgccc attcatcgtc ggcctctggc tgaagcccag      720

cagtggatct gagagaatct tactcaaggc ggcaaatacc cacagttcct cccagctttg      780

cgagcagcag tctgttcact tgggcggagt gtttgaatta caagctggtg cttctgtgtt      840

tgtcaacgtg actgaagcaa gccaagtgat ccacagagtt ggcttctcat cttttggctt      900

actcaaactc ggcggaggca gcgccgccgc tggcctgaac gatatttttg aagcgcagaa      960

aatcgaatgg cacgaacatc accaccatca ccactaataa ggatcc                    1006


<210>  5
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Deduced protein sequence from expression construct 
       TMEM27(125)-sCD40L

<400>  5

Met Leu Trp Leu Leu Phe Phe Leu Val Thr Ala Ile His Ala Glu Leu 
1               5                   10                  15      


Cys Gln Pro Gly Ala Glu Asn Ala Phe Lys Val Arg Leu Ser Ile Arg 
            20                  25                  30          


Thr Ala Leu Gly Asp Lys Ala Tyr Ala Trp Asp Thr Asn Glu Glu Tyr 
        35                  40                  45              


Leu Phe Lys Ala Met Val Ala Phe Ser Met Arg Lys Val Pro Asn Arg 
    50                  55                  60                  


Glu Ala Thr Glu Ile Ser His Val Leu Leu Cys Asn Val Thr Gln Arg 
65                  70                  75                  80  


Val Ser Phe Trp Phe Val Val Thr Asp Pro Ser Lys Asn His Thr Leu 
                85                  90                  95      


Pro Ala Val Glu Val Gln Ser Ala Ile Arg Met Asn Lys Asn Arg Ile 
            100                 105                 110         


Asn Asn Ala Phe Phe Leu Asn Asp Gln Thr Leu Glu Phe Ala Ala Ala 
        115                 120                 125             


Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Ser Gly Gly Gly Met Gln Arg Gly Asp Glu Asp Pro 
145                 150                 155                 160 


Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser 
                165                 170                 175     


Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu 
            180                 185                 190         


Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu 
        195                 200                 205             


Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser 
    210                 215                 220                 


Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly 
225                 230                 235                 240 


Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln 
                245                 250                 255     


Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln 
            260                 265                 270         


Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile 
        275                 280                 285             


His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Gly Gly Gly 
    290                 295                 300                 


Ser Ala Ala Ala Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu 
305                 310                 315                 320 


Trp His Glu His His His His His His 
                325                 


<210>  6
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  6

Ile Arg Thr Ala Leu Gly Asp Lys Ala Tyr Ala Trp Asp Thr Asn 
1               5                   10                  15  


<210>  7
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  7

Arg Lys Val Pro Asn Arg Glu Ala Thr Glu Ile 
1               5                   10      


<210>  8
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  8

Ser Lys Asn His Thr Leu Pro Ala Val Glu Val Gln Ser Ala Ile 
1               5                   10                  15  


<210>  9
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  9

Arg Ile Asn Asn Ala Phe Phe Leu Asn Asp Gln Thr Leu 
1               5                   10              


<210>  10
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  10

Lys Ile Pro Ser Thr Leu Ala Pro Pro Met Asp Pro Ser Val Pro 
1               5                   10                  15  


<210>  11
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  11

Arg Lys Asn Lys Glu Pro Ser Glu Val Asp Asp 
1               5                   10      


<210>  12
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  12

Asn His Thr Leu Pro Ala Val Glu Val Gln Ser Ala Ile Arg Met 
1               5                   10                  15  


