﻿               SEQUENCE LISTING

<110> Medizinische Universität Wien

<120> Means and methods for treating a disease or disorder related to lymphangiogenesis or preventing metastasis

<130> S3116 PCT S3

<150> EP 11 15 5240.2
<151> 2011-02-21

<160> 46

<170> BiSSAP 1.0

<210> 1
<211> 1113
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..1113
<223> /mol_type="DNA"
      /note="cDNA human Wnt-1"
      /organism="Homo sapiens"

<400> 1
atggggctct gggcgctgtt gcctggctgg gtttctgcta cgctgctgct ggcgctggcc      60

gctctgcccg cagccctggc tgccaacagc agtggccgat ggtggggtat tgtgaacgta     120

gcctcctcca cgaacctgct tacagactcc aagagtctgc aactggtact cgagcccagt     180

ctgcagctgt tgagccgcaa acagcggcgt ctgatacgcc aaaatccggg gatcctgcac     240

agcgtgagtg gggggctgca gagtgccgtg cgcgagtgca agtggcagtt ccggaatcgc     300

cgctggaact gtcccactgc tccagggccc cacctcttcg gcaagatcgt caaccgaggc     360

tgtcgagaaa cggcgtttat cttcgctatc acctccgccg gggtcaccca ttcggtggcg     420

cgctcctgct cagaaggttc catcgaatcc tgcacgtgtg actaccggcg gcgcggcccc     480

gggggccccg actggcactg ggggggctgc agcgacaaca ttgacttcgg ccgcctcttc     540

ggccgggagt tcgtggactc cggggagaag gggcgggacc tgcgcttcct catgaacctt     600

cacaacaacg aggcaggccg tacgaccgta ttctccgaga tgcgccagga gtgcaagtgc     660

cacgggatgt ccggctcatg cacggtgcgc acgtgctgga tgcggctgcc cacgctgcgc     720

gccgtgggcg atgtgctgcg cgaccgcttc gacggcgcct cgcgcgtcct gtacggcaac     780

cgcggcagca accgcgcttc gcgggcggag ctgctgcgcc tggagccgga agacccggcc     840

cacaaaccgc cctcccccca cgacctcgtc tacttcgaga aatcgcccaa cttctgcacg     900

tacagcggac gcctgggcac agcaggcacg gcagggcgcg cctgtaacag ctcgtcgccc     960

gcgctggacg gctgcgagct gctctgctgc ggcaggggcc accgcacgcg cacgcagcgc    1020

gtcaccgagc gctgcaactg caccttccac tggtgctgcc acgtcagctg ccgcaactgc    1080

acgcacacgc gcgtactgca cgagtgtctg tga                                 1113


<210> 2
<211> 370
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..370
<223> /mol_type="protein"
      /note="protein human Wnt-1"
      /organism="Homo sapiens"

<400> 2
Met Gly Leu Trp Ala Leu Leu Pro Gly Trp Val Ser Ala Thr Leu Leu 
1               5                   10                   15    
Leu Ala Leu Ala Ala Leu Pro Ala Ala Leu Ala Ala Asn Ser Ser Gly 
            20                   25                  30        
Arg Trp Trp Gly Ile Val Asn Val Ala Ser Ser Thr Asn Leu Leu Thr 
        35                   40                  45            
Asp Ser Lys Ser Leu Gln Leu Val Leu Glu Pro Ser Leu Gln Leu Leu 
    50                   55                  60                
Ser Arg Lys Gln Arg Arg Leu Ile Arg Gln Asn Pro Gly Ile Leu His 
65                   70                  75                  80
Ser Val Ser Gly Gly Leu Gln Ser Ala Val Arg Glu Cys Lys Trp Gln 
                85                   90                  95    
Phe Arg Asn Arg Arg Trp Asn Cys Pro Thr Ala Pro Gly Pro His Leu 
            100                  105                110        
Phe Gly Lys Ile Val Asn Arg Gly Cys Arg Glu Thr Ala Phe Ile Phe 
        115                  120                125            
Ala Ile Thr Ser Ala Gly Val Thr His Ser Val Ala Arg Ser Cys Ser 
    130                  135                140                
Glu Gly Ser Ile Glu Ser Cys Thr Cys Asp Tyr Arg Arg Arg Gly Pro 
145                  150                155                  160
Gly Gly Pro Asp Trp His Trp Gly Gly Cys Ser Asp Asn Ile Asp Phe 
                165                  170                175    
Gly Arg Leu Phe Gly Arg Glu Phe Val Asp Ser Gly Glu Lys Gly Arg 
            180                  185                190        
Asp Leu Arg Phe Leu Met Asn Leu His Asn Asn Glu Ala Gly Arg Thr 
        195                  200                205            
Thr Val Phe Ser Glu Met Arg Gln Glu Cys Lys Cys His Gly Met Ser 
    210                  215                220                
Gly Ser Cys Thr Val Arg Thr Cys Trp Met Arg Leu Pro Thr Leu Arg 
225                  230                235                  240
Ala Val Gly Asp Val Leu Arg Asp Arg Phe Asp Gly Ala Ser Arg Val 
                245                  250                255    
Leu Tyr Gly Asn Arg Gly Ser Asn Arg Ala Ser Arg Ala Glu Leu Leu 
            260                  265                270        
Arg Leu Glu Pro Glu Asp Pro Ala His Lys Pro Pro Ser Pro His Asp 
        275                  280                285            
Leu Val Tyr Phe Glu Lys Ser Pro Asn Phe Cys Thr Tyr Ser Gly Arg 
    290                  295                300                
Leu Gly Thr Ala Gly Thr Ala Gly Arg Ala Cys Asn Ser Ser Ser Pro 
305                  310                315                  320
Ala Leu Asp Gly Cys Glu Leu Leu Cys Cys Gly Arg Gly His Arg Thr 
                325                  330                335    
Arg Thr Gln Arg Val Thr Glu Arg Cys Asn Cys Thr Phe His Trp Cys 
            340                  345                350        
Cys His Val Ser Cys Arg Asn Cys Thr His Thr Arg Val Leu His Glu 
        355                  360                365            
Cys Leu 
    370

<210> 3
<211> 1032
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..1032
<223> /mol_type="DNA"
      /note="cDNA human Wnt-1 without signal peptide sequence"
      /organism="Homo sapiens"

<400> 3
gccaacagca gtggccgatg gtggggtatt gtgaacgtag cctcctccac gaacctgctt      60

acagactcca agagtctgca actggtactc gagcccagtc tgcagctgtt gagccgcaaa     120

cagcggcgtc tgatacgcca aaatccgggg atcctgcaca gcgtgagtgg ggggctgcag     180

agtgccgtgc gcgagtgcaa gtggcagttc cggaatcgcc gctggaactg tcccactgct     240

ccagggcccc acctcttcgg caagatcgtc aaccgaggct gtcgagaaac ggcgtttatc     300

ttcgctatca cctccgccgg ggtcacccat tcggtggcgc gctcctgctc agaaggttcc     360

atcgaatcct gcacgtgtga ctaccggcgg cgcggccccg ggggccccga ctggcactgg     420

gggggctgca gcgacaacat tgacttcggc cgcctcttcg gccgggagtt cgtggactcc     480

ggggagaagg ggcgggacct gcgcttcctc atgaaccttc acaacaacga ggcaggccgt     540

acgaccgtat tctccgagat gcgccaggag tgcaagtgcc acgggatgtc cggctcatgc     600

acggtgcgca cgtgctggat gcggctgccc acgctgcgcg ccgtgggcga tgtgctgcgc     660

gaccgcttcg acggcgcctc gcgcgtcctg tacggcaacc gcggcagcaa ccgcgcttcg     720

cgggcggagc tgctgcgcct ggagccggaa gacccggccc acaaaccgcc ctccccccac     780

gacctcgtct acttcgagaa atcgcccaac ttctgcacgt acagcggacg cctgggcaca     840

gcaggcacgg cagggcgcgc ctgtaacagc tcgtcgcccg cgctggacgg ctgcgagctg     900

ctctgctgcg gcaggggcca ccgcacgcgc acgcagcgcg tcaccgagcg ctgcaactgc     960

accttccact ggtgctgcca cgtcagctgc cgcaactgca cgcacacgcg cgtactgcac    1020

gagtgtctgt ga                                                        1032


<210> 4
<211> 343
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..343
<223> /mol_type="protein"
      /note="protein human Wnt-1 without signal peptide sequence"
      /organism="Homo sapiens"

<400> 4
Ala Asn Ser Ser Gly Arg Trp Trp Gly Ile Val Asn Val Ala Ser Ser 
1               5                   10                   15    
Thr Asn Leu Leu Thr Asp Ser Lys Ser Leu Gln Leu Val Leu Glu Pro 
            20                   25                  30        
Ser Leu Gln Leu Leu Ser Arg Lys Gln Arg Arg Leu Ile Arg Gln Asn 
        35                   40                  45            
Pro Gly Ile Leu His Ser Val Ser Gly Gly Leu Gln Ser Ala Val Arg 
    50                   55                  60                
Glu Cys Lys Trp Gln Phe Arg Asn Arg Arg Trp Asn Cys Pro Thr Ala 
65                   70                  75                  80
Pro Gly Pro His Leu Phe Gly Lys Ile Val Asn Arg Gly Cys Arg Glu 
                85                   90                  95    
Thr Ala Phe Ile Phe Ala Ile Thr Ser Ala Gly Val Thr His Ser Val 
            100                  105                110        
Ala Arg Ser Cys Ser Glu Gly Ser Ile Glu Ser Cys Thr Cys Asp Tyr 
        115                  120                125            
Arg Arg Arg Gly Pro Gly Gly Pro Asp Trp His Trp Gly Gly Cys Ser 
    130                  135                140                
Asp Asn Ile Asp Phe Gly Arg Leu Phe Gly Arg Glu Phe Val Asp Ser 
145                  150                155                  160
Gly Glu Lys Gly Arg Asp Leu Arg Phe Leu Met Asn Leu His Asn Asn 
                165                  170                175    
Glu Ala Gly Arg Thr Thr Val Phe Ser Glu Met Arg Gln Glu Cys Lys 
            180                  185                190        
Cys His Gly Met Ser Gly Ser Cys Thr Val Arg Thr Cys Trp Met Arg 
        195                  200                205            
Leu Pro Thr Leu Arg Ala Val Gly Asp Val Leu Arg Asp Arg Phe Asp 
    210                  215                220                
Gly Ala Ser Arg Val Leu Tyr Gly Asn Arg Gly Ser Asn Arg Ala Ser 
225                  230                235                  240
Arg Ala Glu Leu Leu Arg Leu Glu Pro Glu Asp Pro Ala His Lys Pro 
                245                  250                255    
Pro Ser Pro His Asp Leu Val Tyr Phe Glu Lys Ser Pro Asn Phe Cys 
            260                  265                270        
Thr Tyr Ser Gly Arg Leu Gly Thr Ala Gly Thr Ala Gly Arg Ala Cys 
        275                  280                285            
Asn Ser Ser Ser Pro Ala Leu Asp Gly Cys Glu Leu Leu Cys Cys Gly 
    290                  295                300                
Arg Gly His Arg Thr Arg Thr Gln Arg Val Thr Glu Arg Cys Asn Cys 
305                  310                315                  320
Thr Phe His Trp Cys Cys His Val Ser Cys Arg Asn Cys Thr His Thr 
                325                  330                335    
Arg Val Leu His Glu Cys Leu 
            340            

<210> 5
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-1"
      /organism="artificial sequences"

<400> 5
cacgacctcg tctacttcga g                                              21


<210> 6
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-1"
      /organism="artificial sequences"

<400> 6
acagacactc gtgcagtacg c                                              21


<210> 7
<211> 19
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..19
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR  - Wnt-2"
      /organism="artificial sequences"

<400> 7
ccagcctttt ggcagggtc                                                 19


<210> 8
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-2"
      /organism="artificial sequences"

<400> 8
gcatgtcctg agagtccatg                                                20


<210> 9
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-2b"
      /organism="artificial sequences"

<400> 9
aagatggtgc caacttcacc g                                              21


<210> 10
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-2b"
      /organism="artificial sequences"

<400> 10
ctgccttctt gggggctttg c                                              21


<210> 11
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-3"
      /organism="artificial sequences"

<400> 11
tgaacaagca caacaacgag                                                20


<210> 12
<211> 19
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..19
<223> /mol_type="DNA"
      /note="reverse prime used for RT-PCR - Wnt-3"
      /organism="artificial sequences"

<400> 12
cagtggcatt tttccttcc                                                 19


<210> 13
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="foward primer used for RT-PCR - Wnt-3a"
      /organism="artificial sequences"

<400> 13
caggaactac gtggagatca tg                                             22


<210> 14
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR -Wnt-3a"
      /organism="artificial sequences"

<400> 14
ccatcccacc aaactcgatg tc                                             22


<210> 15
<211> 18
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..18
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-4"
      /organism="artificial sequences"

<400> 15
ccttctcaca gtcgtttg                                                  18


<210> 16
<211> 19
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..19
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-4"
      /organism="artificial sequences"

<400> 16
cacagccgtc gatggcctt                                                 19


<210> 17
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-5a"
      /organism="artificial sequences"

<400> 17
gggaggttgg cttgaacata                                                20


<210> 18
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-5a"
      /organism="artificial sequences"

<400> 18
gaatggcacg caattacctt                                                20


<210> 19
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-6"
      /organism="artificial sequences"

<400> 19
tggtgctgcg tagtacagtg c                                              21


<210> 20
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-6"
      /organism="artificial sequences"

<400> 20
ccatcctgtg gccagcagtt c                                              21


<210> 21
<211> 24
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-7a"
      /organism="artificial sequences"

<400> 21
gctgcctggg ccacctcttt ctca                                           24


<210> 22
<211> 24
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-7a"
      /organism="artificial sequences"

<400> 22
cccggtggta caggccttgc ttct                                           24


<210> 23
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-8b"
      /organism="artificial sequences"

<400> 23
atgtctttgg ggttggttcc tag                                            23


<210> 24
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-8b"
      /organism="artificial sequences"

<400> 24
ttgctaggag gaagaaggtc ag                                             22


<210> 25
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-10b"
      /organism="artificial sequences"

<400> 25
gaatgcgaat ccacaacaac ag                                             22


<210> 26
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-10b"
      /organism="artificial sequences"

<400> 26
ttgcggttgt gggtatcaat gaa                                            23


<210> 27
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-11"
      /organism="artificial sequences"

<400> 27
gtaagtgcca tggggtgtct                                                20


<210> 28
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-11"
      /organism="artificial sequences"

<400> 28
gcttccaagt gaaggcaaag                                                20


<210> 29
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Wnt-16"
      /organism="artificial sequences"

<400> 29
tgctcgtgct gttcccctac                                                20


<210> 30
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Wnt-16"
      /organism="artificial sequences"

<400> 30
atcatgcagt tccatctctc                                                20


<210> 31
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-1"
      /organism="artificial sequences"

<400> 31
gcccatgagc ccggacttca c                                              21


<210> 32
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-1"
      /organism="artificial sequences"

<400> 32
tcagactgta gtctcccctt g                                              21


<210> 33
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-3"
      /organism="artificial sequences"

<400> 33
aaggcttcca cagtgacaca agg                                            23


<210> 34
<211> 25
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..25
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-3"
      /organism="artificial sequences"

<400> 34
agaggagaga aaccccaact accac                                          25


<210> 35
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-4"
      /organism="artificial sequences"

<400> 35
ggaaatggtt gggtgaagcc tg                                             22


<210> 36
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-4"
      /organism="artificial sequences"

<400> 36
tttttgatgc tggggtcggg                                                20


<210> 37
<211> 24
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-5"
      /organism="artificial sequences"

<400> 37
gggcccgttc gtgtgcaagt gtcg                                           24


<210> 38
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-5"
      /organism="artificial sequences"

<400> 38
atgccggcgg ccaggaacca g                                              21


<210> 39
<211> 24
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-6"
      /organism="artificial sequences"

<400> 39
agtcttcagc ggcttgtatc ttgt                                           24


<210> 40
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-6"
      /organism="artificial sequences"

<400> 40
gctccgtccg ctttcacctc t                                              21


<210> 41
<211> 24
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-7"
      /organism="artificial sequences"

<400> 41
acagacttag ccacagcagc aagg                                           24


<210> 42
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-7"
      /organism="artificial sequences"

<400> 42
tttccaaatc acccctcgcc                                                20


<210> 43
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-9"
      /organism="artificial sequences"

<400> 43
ttcttctcca ccgccttcac                                                20


<210> 44
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-9"
      /organism="artificial sequences"

<400> 44
caggatgacg atggtcttga g                                              21


<210> 45
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="forward primer used for RT-PCR - Fzd-10"
      /organism="artificial sequences"

<400> 45
tatcgggctc ttctctgtgc                                                20


<210> 46
<211> 20
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="reverse primer used for RT-PCR - Fzd-10"
      /organism="artificial sequences"

<400> 46
gactgggcag ggatctcata                                                20


