                         SEQUENCE LISTING

<110>  Consejo Superior de Investigaciones Cientificas
 
<120>  Metodo de amplificacion de ADN basado en los origenes de 
       replicacion del bacteriofago Phi29 y secuencias nucleotidicas 
       asociadas

<130>  ES1641.842

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  5833
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pETORPHI.

<400>  1
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgc      180

atgcaggcct ctgcagtcga cgggcccggg atccgatcca atcagtggct gtgcgacaca      240

gacgaagcgc taaaacgtgg gattctgtgt cgttttatgt tgttcattga caaacctatc      300

tagtaggtct atagattgat atattaagta gtagtttccc tccctgatca tggtatgccg      360

aggatcgacg gattatgtcg atattaggag aatggtatca tgtgagggtg ggggcttact      420

ttaaagtagg gtacagcgac aacatacacc atttccccat tgaccgacta tcttcgacaa      480

gaatctaaca actaaatcac gactatatac ctatactatt tattatcatc aatttgtcga      540

aaagggtaga caaactatcg tttaacatgt tatactataa tagaagtaag gtaataagac      600

aaccaatcat aggaggcacg agattgatct agatgcattc gcgaggtacc gagctcgaat      660

tcggatcccg acccatttgc tgtccaccag tcatgctagc catatggctg ccgcgcggca      720

ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta      780

aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg      840

agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg      900

gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg      960

aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag     1020

gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg     1080

cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg     1140

gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga     1200

tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat     1260

gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc     1320

cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt     1380

cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc     1440

tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat     1500

caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa     1560

gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg     1620

gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt     1680

gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga     1740

ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca     1800

ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat     1860

caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa     1920

accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag     1980

atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc     2040

tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc     2100

atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct     2160

cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc     2220

accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag     2280

ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag     2340

ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt     2400

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     2460

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     2520

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     2580

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     2640

tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg     2700

gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac     2760

gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg     2820

tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg     2880

gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa     2940

gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg     3000

tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag     3060

catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga     3120

ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca     3180

gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg     3240

tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga     3300

tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc     3360

ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg     3420

cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca     3480

tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg     3540

taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct     3600

cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     3660

cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     3720

accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     3780

acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     3840

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     3900

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     3960

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     4020

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     4080

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     4140

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg     4200

gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     4260

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     4320

gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga     4380

acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac     4440

agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga     4500

ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg     4560

caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg     4620

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg     4680

ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca     4740

tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct     4800

gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt     4860

cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca     4920

cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct     4980

gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc     5040

actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt     5100

attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac     5160

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat     5220

aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta     5280

attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     5340

cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc     5400

gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc     5460

ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag     5520

tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga     5580

tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc     5640

actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa     5700

cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt     5760

agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc     5820

gtcccattcg cca                                                        5833


<210>  2
<211>  5666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pETORPHI68L.

<400>  2
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgc      180

atgcaggcct ctgcagtcga cgggcccggg atccgatcca atcagtggct gtgcgacaca      240

gacgaagcgc taaaacgtgg gattctgtgt cgttttatgt tgttcattga caaacctatc      300

tagtaggtct atagattgat atattaagta gtagtttccc tccctgatca tggtatgccg      360

aggatcgacg gattatgtcg atattaggag aatggtatca tgtgagggtg ggggcttact      420

ttaaagtaag cccccaccct cacatgatac cattctccta atatcgacat aatccgtcga      480

tcctcggcat aattcggatc ccgacccatt tgctgtccac cagtcatgct agccatatgg      540

ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat gatggctgct gcccatggta      600

tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa      660

ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga      720

cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac      780

atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca      900

ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag      960

gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg     1020

cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt     1080

aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt     1140

gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga     1200

gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat     1260

tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa     1320

atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt     1380

cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat     1440

taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc     1500

ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga     1560

agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct     1620

gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata     1680

tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc     1740

cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt     1800

tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt     1860

tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc     1920

gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt     1980

gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt     2040

gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     2100

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg     2160

caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag     2220

ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta     2280

tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt     2340

tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg     2400

gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg     2460

agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt     2520

cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga     2580

tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat     2640

gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca     2700

ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat     2760

ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc     2820

ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa     2880

cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc     2940

ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct     3000

ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac     3060

aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg     3120

cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag     3180

cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg     3240

gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct     3300

taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata     3360

ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact     3420

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta     3480

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag     3540

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc     3600

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta     3660

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg     3720

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc     3780

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac     3840

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     3900

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     3960

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     4020

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     4080

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     4140

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     4200

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa taaaactgtc     4260

tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg     4320

ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg     4380

cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc     4440

agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt     4500

cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac     4560

tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt     4620

agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg     4680

gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc     4740

tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg     4800

taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc     4860

ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa     4920

attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc     4980

catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa     5040

atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt     5100

tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa     5160

taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt     5220

tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     5280

tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag     5340

tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     5400

tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga     5460

ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     5520

gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     5580

cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc     5640

cgctacaggg cgcgtcccat tcgcca                                          5666


<210>  3
<211>  5871
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pETORPHIBae.

<400>  3
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgc      180

atgcaggcct ctgcagtcga cgggcccggg atccgatcca atcagtggct gtgcgacaca      240

gacgaagcgc taaaacgtgg gattctgtgt cgttttatgt tgttcattga caaacctatc      300

tagtaggtct atagattgat atattaagta gtagtttccc tccctgatca tggtatgccg      360

aggatcgacg gattatgtcg atattaggag aatggtatca tgtgagggtg ggggcttact      420

tttctcctaa ttatttgact gtcgtatctg tcatcaacca aaagtagggt acagcgacaa      480

catacaccat ttccccattg accgactatc ttcgacaaga atctaacaac taaatcacga      540

ctatatacct atactattta ttatcatcaa tttgtcgaaa agggtagaca aactatcgtt      600

taacatgtta tactataata gaagtaaggt aataagacaa ccaatcatag gaggcacgag      660

attgatctag atgcattcgc gaggtaccga gctcgaattc ggatcccgac ccatttgctg      720

tccaccagtc atgctagcca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg      780

atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa attatttcta      840

gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat      900

cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc      960

ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg     1020

gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt     1080

gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct     1140

actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca     1200

ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat     1260

tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct     1320

cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg     1380

aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac     1440

tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc     1500

cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg     1560

tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg     1620

cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg     1680

tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca     1740

tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg     1800

cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc     1860

tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac     1920

gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg     1980

gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg     2040

ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata     2100

ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc     2160

tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca     2220

atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa     2280

ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac     2340

tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg     2400

ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg     2460

ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag     2520

gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg     2580

atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact     2640

ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca     2700

cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt     2760

actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa     2820

acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg     2880

gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc     2940

tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg     3000

atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt     3060

aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt     3120

atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa     3180

aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa     3240

ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct     3300

gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac     3360

atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc     3420

cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt     3480

agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag     3540

tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag     3600

gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc     3660

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg     3720

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct     3780

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     3840

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     3900

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     3960

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     4020

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     4080

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     4140

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     4200

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc     4260

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     4320

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     4380

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     4440

tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga     4500

gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg     4560

atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc     4620

gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg     4680

ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc     4740

cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc     4800

ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg     4860

atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta     4920

acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg     4980

atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa     5040

tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg     5100

ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa     5160

tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt     5220

cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc     5280

agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt     5340

gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca     5400

cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc     5460

tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc     5520

gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac     5580

tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca     5640

ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg     5700

agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag     5760

aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc     5820

accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a              5871


<210>  4
<211>  3110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pUC57ORPHI.

<400>  4
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tctcgtgcct cctatgattg gttgtcttat taccttactt ctattatagt      480

ataacatgtt aaacgatagt ttgtctaccc ttttcgacaa attgatgata ataaatagta      540

taggtatata gtcgtgattt agttgttaga ttcttgtcga agatagtcgg tcaatgggga      600

aatggtgtat gttgtcgctg taccctactt taaagtaagc ccccaccctc acatgatacc      660

attctcctaa tatcgacata atccgtcgat cctcggcata ccatgatcag ggagggaaac      720

tactacttaa tatatcaatc tatagaccta ctagataggt ttgtcaatga acaacataaa      780

acgacacaga atcccacgtt ttagcgcttc gtctgtgtcg cacagccact gatcggatcc      840

cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt      900

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa      960

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact     1020

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc     1080

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg     1140

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     1200

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     1260

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     1320

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     1380

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     1440

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     1500

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     1560

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     1620

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     1680

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt     1740

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     1800

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     1860

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     1920

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     1980

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     2040

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     2100

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     2160

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     2220

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     2280

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     2340

tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat     2400

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     2460

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     2520

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     2580

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     2640

accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt     2700

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     2760

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     2820

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     2880

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     2940

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     3000

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat     3060

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc                3110


<210>  5
<211>  68
<212>  DNA
<213>  Bacteriophage phi-29


<220>
<221>  variation
<222>  (1)..(1)
<223>  La adenina en la posicion 1 esta fosforilada en 5'.

<220>
<221>  variation
<222>  (1)..(1)
<223>  La adenina en la posicion 1 lleva un grupo propilo.

<220>
<221>  variation
<222>  (1)..(1)
<223>  La adenina en la posicion 1 esta biotinilada.

<400>  5
aaagtaagcc cccaccctca catgatacca ttctcctaat atcgacataa tccgtcgatc       60

ctcggcat                                                                68


<210>  6
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotido con los 68 nucleotidos del extremo izquierdo del ADN del bacteriofago Phi-29 en 3' y el extremo cohesivo de la    diana de EcoRI en 5'.


<220>
<221>  variation
<222>  (1)..(1)
<223>  La adenina en la posicion 1 esta fosforilada en 5'.

<400>  6
aattatgccg aggatcgacg gattatgtcg atattaggag aatggtatca tgtgagggtg       60

ggggcttact tt                                                           72


<210>  7
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotido con los 68 nucleotidos del extremo izquierdo del ADN del bacteriofago Phi-29 en 3' y el extremo cohesivo de la        diana de BsmBI en 5'.


<220>
<221>  variation
<222>  (1)..(1)
<223>  La adenina en la posicion 1 esta fosforilada en 5'.

<400>  7
atccatgccg aggatcgacg gattatgtcg atattaggag aatggtatca tgtgagggtg       60

ggggcttact tt                                                           72


<210>  8
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotido con los 68 nucleotidos del extremo izquierdo del ADN del bacteriofago Phi-29 en 3' y el extremo cohesivo de la        diana de Eco109I en 5'.


<220>
<221>  variation
<222>  (1)..(1)
<223>  La guanina en la posicion 1 esta fosforilada en 5'.

<400>  8
gccatgccga ggatcgacgg attatgtcga tattaggaga atggtatcat gtgagggtgg       60

gggcttactt t                                                            71


<210>  9
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotido directo para generar la secuencia diana de 
       restriccion de BaeI insertada en la secuencia diana de 
       restriccion de DraI de pETORPHI.

<400>  9
tctcctaatt atttgactgt cgtatctgtc atcaacca                               38


<210>  10
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotido inverso para generar la secuencia diana de 
       restriccion de BaeI insertada en la secuencia diana de 
       restriccion de DraI en pETORPHI.

<400>  10
tggttgatga cagatacgac agtcaaataa ttaggaga                               38


<210>  11
<211>  5368
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pET28b.

<400>  11
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt      180

cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat      240

ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg      300

tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac      360

aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg      420

gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg      480

acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg      540

tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac      600

cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc      660

aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt      720

cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca      780

gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg      840

gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg      900

gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg      960

attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt     1020

aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc     1080

gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc     1140

attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt     1200

ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat     1260

gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg     1320

ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa     1380

tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg     1440

tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg     1500

gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc     1560

gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc     1620

ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc     1680

ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg     1740

gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc     1800

gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa     1860

cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag     1920

agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact     1980

tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat     2040

tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt     2100

cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg     2160

cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct     2220

gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc     2280

gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac     2340

atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg     2400

caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac     2460

atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat     2520

ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt     2580

aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa     2640

tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg     2700

ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga     2760

acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct     2820

cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac     2880

agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt     2940

tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg     3000

cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa     3060

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca     3120

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     3180

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     3240

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     3300

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     3360

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     3420

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     3480

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     3540

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     3600

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     3660

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     3720

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     3780

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     3840

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     3900

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg     3960

tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct     4020

tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct     4080

cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg     4140

ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg     4200

gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt     4260

actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta     4320

ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc     4380

cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc     4440

gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag     4500

cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca     4560

ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg     4620

aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt     4680

gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa     4740

aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag     4800

tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca     4860

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat     4920

tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga     4980

aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc     5040

agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac     5100

cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc     5160

gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg     5220

gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag     5280

ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc     5340

gccgctacag ggcgcgtccc attcgcca                                        5368


<210>  12
<211>  191
<212>  DNA
<213>  Bacteriophage phi-29

<400>  12
aaagtaagcc cccaccctca catgatacca ttctcctaat atcgacataa tccgtcgatc       60

ctcggcatac catgatcagg gagggaaact actacttaat atatcaatct atagacctac      120

tagataggtt tgtcaatgaa caacataaaa cgacacagaa tcccacgttt tagcgcttcg      180

tctgtgtcgc a                                                           191


<210>  13
<211>  194
<212>  DNA
<213>  Bacteriophage phi-29

<400>  13
aaagtagggt acagcgacaa catacaccat ttccccattg accgactatc ttcgacaaga       60

atctaacaac taaatcacga ctatatacct atactattta ttatcatcaa tttgtcgaaa      120

agggtagaca aactatcgtt taacatgtta tactataata gaagtaaggt aataagacaa      180

ccaatcatag gagg                                                        194


<210>  14
<211>  4733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEYFP-N1

<400>  14
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg      660

gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc      720

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc      780

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg      840

cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc      900

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc      960

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     1020

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     1080

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     1140

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     1200

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     1260

ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag     1320

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     1380

gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg     1440

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa     1500

tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     1560

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     1620

ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc     1680

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc     1740

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag     1800

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc     1860

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa     1920

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg     1980

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt     2040

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc     2100

gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     2160

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     2220

tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt     2280

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     2340

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     2400

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     2460

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     2520

ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     2580

taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     2640

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     2700

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     2760

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca     2820

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     2880

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     2940

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     3000

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     3060

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     3120

ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc     3180

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     3240

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     3300

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     3360

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     3420

tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag     3480

atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg     3540

ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg     3600

ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa     3660

taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt     3720

cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg     3780

tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca     3840

acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt     3900

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     3960

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     4020

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     4080

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     4140

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     4200

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     4260

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     4320

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     4380

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     4440

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     4500

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     4560

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     4620

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     4680

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat            4733


<210>  15
<211>  4733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEYFP-N1Bsm

<400>  15
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg      660

gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc      720

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc      780

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg      840

cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc      900

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc      960

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     1020

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     1080

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     1140

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     1200

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     1260

ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag     1320

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     1380

gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg     1440

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa     1500

tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     1560

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     1620

ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc     1680

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc     1740

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag     1800

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc     1860

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa     1920

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg     1980

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt     2040

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc     2100

gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     2160

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     2220

tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt     2280

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     2340

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     2400

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     2460

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     2520

ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     2580

taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     2640

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     2700

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     2760

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca     2820

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     2880

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     2940

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     3000

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     3060

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     3120

ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc     3180

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     3240

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     3300

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     3360

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     3420

tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag     3480

atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg     3540

ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg     3600

ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa     3660

taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt     3720

cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg     3780

tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca     3840

acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt     3900

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     3960

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     4020

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     4080

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     4140

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     4200

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     4260

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     4320

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     4380

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     4440

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     4500

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     4560

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     4620

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     4680

tctttcctgc gttatcccct gcgtctctgg ataaccgtat taccgccatg cat            4733


