                                SEQUENCE LISTING

<110> Ambasudhan, Rajesh
      Ding, Sheng
      The Scripps Research Institute

<120> Direct Reprogramming of Human
  Fibroblasts to Functional Neurons Under Defined Conditions
  

<130> 77103-832289

<140>  PCTUS1227313
<141>  2012-03-01


<150> US 61/448,147       
<151> 2011-03-01  

<160> 11

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic human mature miR-124 microRNA sequence
      hsa-miR-124, miRBase Accession No. MIMAT0000422

<400> 1
uaaggcacgc ggugaaugcc                                             20

<210> 2
<211> 1184
<212> PRT
<213> Homo sapiens

<220> 
<223> human myelin transcription factor 1-like (MYT1L)
      transcription factor, neural zinc finger 1 (NZF1)

<400> 2
Met Glu Val Asp Thr Glu Glu Lys Arg His Arg Thr Arg Ser Lys Gly
 1               5                  10                  15      
Val Arg Val Pro Val Glu Pro Ala Ile Gln Glu Leu Phe Ser Cys Pro
            20                  25                  30          
Thr Pro Gly Cys Asp Gly Ser Gly His Val Ser Gly Lys Tyr Ala Arg
        35                  40                  45              
His Arg Ser Val Tyr Gly Cys Pro Leu Ala Lys Lys Arg Lys Thr Gln
    50                  55                  60                  
Asp Lys Gln Pro Gln Glu Pro Ala Pro Lys Arg Lys Pro Phe Ala Val
65                  70                  75                  80  
Lys Ala Asp Ser Ser Ser Val Asp Glu Cys Asp Asp Ser Asp Gly Thr
                85                  90                  95      
Glu Asp Met Asp Glu Lys Glu Glu Asp Glu Gly Glu Glu Tyr Ser Glu
            100                 105                 110         
Asp Asn Asp Glu Pro Gly Asp Glu Asp Glu Glu Asp Glu Glu Gly Asp
        115                 120                 125             
Arg Glu Glu Glu Glu Glu Ile Glu Glu Glu Asp Glu Asp Asp Asp Glu
    130                 135                 140                 
Asp Gly Glu Asp Val Glu Asp Glu Glu Glu Glu Glu Glu Glu Glu Glu
145                 150                 155                 160 
Glu Glu Glu Glu Glu Glu Glu Asn Glu Asp His Gln Met Asn Cys His
                165                 170                 175     
Asn Thr Arg Ile Met Gln Asp Thr Glu Lys Asp Asp Asn Asn Asn Asp
            180                 185                 190         
Glu Tyr Asp Asn Tyr Asp Glu Leu Val Ala Lys Ser Leu Leu Asn Leu
        195                 200                 205             
Gly Lys Ile Ala Glu Asp Ala Ala Tyr Arg Ala Arg Thr Glu Ser Glu
    210                 215                 220                 
Met Asn Ser Asn Thr Ser Asn Ser Leu Glu Asp Asp Ser Asp Lys Asn
225                 230                 235                 240 
Glu Asn Leu Gly Arg Lys Ser Glu Leu Ser Leu Asp Leu Asp Ser Asp
                245                 250                 255     
Val Val Arg Glu Thr Val Asp Ser Leu Lys Leu Leu Ala Gln Gly His
            260                 265                 270         
Gly Val Val Leu Ser Glu Asn Met Asn Asp Arg Asn Tyr Ala Asp Ser
        275                 280                 285             
Met Ser Gln Gln Asp Ser Arg Asn Met Asn Tyr Val Met Leu Gly Lys
    290                 295                 300                 
Pro Met Asn Asn Gly Leu Met Glu Lys Met Val Glu Glu Ser Asp Glu
305                 310                 315                 320 
Glu Val Cys Leu Ser Ser Leu Glu Cys Leu Arg Asn Gln Cys Phe Asp
                325                 330                 335     
Leu Ala Arg Lys Leu Ser Glu Thr Asn Pro Gln Glu Arg Asn Pro Gln
            340                 345                 350         
Gln Asn Met Asn Ile Arg Gln His Val Arg Pro Glu Glu Asp Phe Pro
        355                 360                 365             
Gly Arg Thr Pro Asp Arg Asn Tyr Ser Asp Met Leu Asn Leu Met Arg
    370                 375                 380                 
Leu Glu Glu Gln Leu Ser Pro Arg Ser Arg Val Phe Ala Ser Cys Ala
385                 390                 395                 400 
Lys Glu Asp Gly Cys His Glu Arg Asp Asp Asp Thr Thr Ser Val Asn
                405                 410                 415     
Ser Asp Arg Ser Glu Glu Val Phe Asp Met Thr Lys Gly Asn Leu Thr
            420                 425                 430         
Leu Leu Glu Lys Ala Ile Ala Leu Glu Thr Glu Arg Ala Lys Ala Met
        435                 440                 445             
Arg Glu Lys Met Ala Met Glu Ala Gly Arg Arg Asp Asn Met Arg Ser
    450                 455                 460                 
Tyr Glu Asp Gln Ser Pro Arg Gln Leu Pro Gly Glu Asp Arg Lys Pro
465                 470                 475                 480 
Lys Ser Ser Asp Ser His Val Lys Lys Pro Tyr Tyr Asp Pro Ser Arg
                485                 490                 495     
Thr Glu Lys Lys Glu Ser Lys Cys Pro Thr Pro Gly Cys Asp Gly Thr
            500                 505                 510         
Gly His Val Thr Gly Leu Tyr Pro His His Arg Ser Leu Ser Gly Cys
        515                 520                 525             
Pro His Lys Asp Arg Val Pro Pro Glu Ile Leu Ala Met His Glu Ser
    530                 535                 540                 
Val Leu Lys Cys Pro Thr Pro Gly Cys Thr Gly Arg Gly His Val Asn
545                 550                 555                 560 
Ser Asn Arg Asn Ser His Arg Ser Leu Ser Gly Cys Pro Ile Ala Ala
                565                 570                 575     
Ala Glu Lys Leu Ala Lys Ala Gln Glu Lys His Gln Ser Cys Asp Val
            580                 585                 590         
Ser Lys Ser Ser Gln Ala Ser Asp Arg Val Leu Arg Pro Met Cys Phe
        595                 600                 605             
Val Lys Gln Leu Glu Ile Pro Gln Tyr Gly Tyr Arg Asn Asn Val Pro
    610                 615                 620                 
Thr Thr Thr Pro Arg Ser Asn Leu Ala Lys Glu Leu Glu Lys Tyr Ser
625                 630                 635                 640 
Lys Thr Ser Phe Glu Tyr Asn Ser Tyr Asp Asn His Thr Tyr Gly Lys
                645                 650                 655     
Arg Ala Ile Ala Pro Lys Val Gln Thr Arg Asp Ile Ser Pro Lys Gly
            660                 665                 670         
Tyr Asp Asp Ala Lys Arg Tyr Cys Lys Asp Pro Ser Pro Ser Ser Ser
        675                 680                 685             
Ser Thr Ser Ser Tyr Ala Pro Ser Ser Ser Ser Asn Leu Ser Cys Gly
    690                 695                 700                 
Gly Gly Ser Ser Ala Ser Ser Thr Cys Ser Lys Ser Ser Phe Asp Tyr
705                 710                 715                 720 
Thr His Asp Met Glu Ala Ala His Met Ala Ala Thr Ala Ile Leu Asn
                725                 730                 735     
Leu Ser Thr Arg Cys Arg Glu Met Pro Gln Asn Leu Ser Thr Lys Pro
            740                 745                 750         
Gln Asp Leu Cys Ala Thr Arg Asn Pro Asp Met Glu Val Asp Glu Asn
        755                 760                 765             
Gly Thr Leu Asp Leu Ser Met Asn Lys Gln Arg Pro Arg Asp Ser Cys
    770                 775                 780                 
Cys Pro Ile Leu Thr Pro Leu Glu Pro Met Ser Pro Gln Gln Gln Ala
785                 790                 795                 800 
Val Met Asn Asn Arg Cys Phe Gln Leu Gly Glu Gly Asp Cys Trp Asp
                805                 810                 815     
Leu Pro Val Asp Tyr Thr Lys Met Lys Pro Arg Arg Ile Asp Glu Asp
            820                 825                 830         
Glu Ser Lys Asp Ile Thr Pro Glu Asp Leu Asp Pro Phe Gln Glu Ala
        835                 840                 845             
Leu Glu Glu Arg Arg Tyr Pro Gly Glu Val Thr Ile Pro Ser Pro Lys
    850                 855                 860                 
Pro Lys Tyr Pro Gln Cys Lys Glu Ser Lys Lys Asp Leu Ile Thr Leu
865                 870                 875                 880 
Ser Gly Cys Pro Leu Ala Asp Lys Ser Ile Arg Ser Met Leu Ala Thr
                885                 890                 895     
Ser Ser Gln Glu Leu Lys Cys Pro Thr Pro Gly Cys Asp Gly Ser Gly
            900                 905                 910         
His Ile Thr Gly Asn Tyr Ala Ser His Arg Ser Leu Ser Gly Cys Pro
        915                 920                 925             
Arg Ala Lys Lys Ser Gly Ile Arg Ile Ala Gln Ser Lys Glu Asp Lys
    930                 935                 940                 
Glu Asp Gln Glu Pro Ile Arg Cys Pro Val Pro Gly Cys Asp Gly Gln
945                 950                 955                 960 
Gly His Ile Thr Gly Lys Tyr Ala Ser His Arg Ser Ala Ser Gly Cys
                965                 970                 975     
Pro Leu Ala Ala Lys Arg Gln Lys Asp Gly Tyr Leu Asn Gly Ser Gln
            980                 985                 990         
Phe Ser Trp Lys Ser Val Lys Thr Glu Gly Met Ser Cys Pro Thr Pro
        995                 1000                1005            
Gly Cys Asp Gly Ser Gly His Val Ser Gly Ser Phe Leu Thr His Arg
    1010                1015                1020                
Ser Leu Ser Gly Cys Pro Arg Ala Thr Ser Ala Met Lys Lys Ala Lys
1025                1030                1035                1040
Leu Ser Gly Glu Gln Met Leu Thr Ile Lys Gln Arg Ala Ser Asn Gly
                1045                1050                1055    
Ile Glu Asn Asp Glu Glu Ile Lys Gln Leu Asp Glu Glu Ile Lys Glu
            1060                1065                1070        
Leu Asn Glu Ser Asn Ser Gln Met Glu Ala Asp Met Ile Lys Leu Arg
        1075                1080                1085            
Thr Gln Ile Thr Thr Met Glu Ser Asn Leu Lys Thr Ile Glu Glu Glu
    1090                1095                1100                
Asn Lys Val Ile Glu Gln Gln Asn Glu Ser Leu Leu His Glu Leu Ala
1105                1110                1115                1120
Asn Leu Ser Gln Ser Leu Ile His Ser Leu Ala Asn Ile Gln Leu Pro
                1125                1130                1135    
His Met Asp Pro Ile Asn Glu Gln Asn Phe Asp Ala Tyr Val Thr Thr
            1140                1145                1150        
Leu Thr Glu Met Tyr Thr Asn Gln Asp Arg Tyr Gln Ser Pro Glu Asn
        1155                1160                1165            
Lys Ala Leu Leu Glu Asn Ile Lys Gln Ala Val Arg Gly Ile Gln Val
    1170                1175                1180                


<210> 3
<211> 443
<212> PRT
<213> Homo sapiens

<220> 
<223> human Brain-2 (BRN2) transcription factor, POU domain,
      class 3, transcription factor 2 (POU3F2), Oct-7

<400> 3
Met Ala Thr Ala Ala Ser Asn His Tyr Ser Leu Leu Thr Ser Ser Ala
 1               5                  10                  15      
Ser Ile Val His Ala Glu Pro Pro Gly Gly Met Gln Gln Gly Ala Gly
            20                  25                  30          
Gly Tyr Arg Glu Ala Gln Ser Leu Val Gln Gly Asp Tyr Gly Ala Leu
        35                  40                  45              
Gln Ser Asn Gly His Pro Leu Ser His Ala His Gln Trp Ile Thr Ala
    50                  55                  60                  
Leu Ser His Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly
65                  70                  75                  80  
Gly Gly Gly Gly Gly Gly Gly Gly Asp Gly Ser Pro Trp Ser Thr Ser
                85                  90                  95      
Pro Leu Gly Gln Pro Asp Ile Lys Pro Ser Val Val Val Gln Gln Gly
            100                 105                 110         
Gly Arg Gly Asp Glu Leu His Gly Pro Gly Ala Leu Gln Gln Gln His
        115                 120                 125             
Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln
    130                 135                 140                 
Gln Gln Gln Gln Gln Arg Pro Pro His Leu Val His His Ala Ala Asn
145                 150                 155                 160 
His His Pro Gly Pro Gly Ala Trp Arg Ser Ala Ala Ala Ala Ala His
                165                 170                 175     
Leu Pro Pro Ser Met Gly Ala Ser Asn Gly Gly Leu Leu Tyr Ser Gln
            180                 185                 190         
Pro Ser Phe Thr Val Asn Gly Met Leu Gly Ala Gly Gly Gln Pro Ala
        195                 200                 205             
Gly Leu His His His Gly Leu Arg Asp Ala His Asp Glu Pro His His
    210                 215                 220                 
Ala Asp His His Pro His Pro His Ser His Pro His Gln Gln Pro Pro
225                 230                 235                 240 
Pro Pro Pro Pro Pro Gln Gly Pro Pro Gly His Pro Gly Ala His His
                245                 250                 255     
Asp Pro His Ser Asp Glu Asp Thr Pro Thr Ser Asp Asp Leu Glu Gln
            260                 265                 270         
Phe Ala Lys Gln Phe Lys Gln Arg Arg Ile Lys Leu Gly Phe Thr Gln
        275                 280                 285             
Ala Asp Val Gly Leu Ala Leu Gly Thr Leu Tyr Gly Asn Val Phe Ser
    290                 295                 300                 
Gln Thr Thr Ile Cys Arg Phe Glu Ala Leu Gln Leu Ser Phe Lys Asn
305                 310                 315                 320 
Met Cys Lys Leu Lys Pro Leu Leu Asn Lys Trp Leu Glu Glu Ala Asp
                325                 330                 335     
Ser Ser Ser Gly Ser Pro Thr Ser Ile Asp Lys Ile Ala Ala Gln Gly
            340                 345                 350         
Arg Lys Arg Lys Lys Arg Thr Ser Ile Glu Val Ser Val Lys Gly Ala
        355                 360                 365             
Leu Glu Ser His Phe Leu Lys Cys Pro Lys Pro Ser Ala Gln Glu Ile
    370                 375                 380                 
Thr Ser Leu Ala Asp Ser Leu Gln Leu Glu Lys Glu Val Val Arg Val
385                 390                 395                 400 
Trp Phe Cys Asn Arg Arg Gln Lys Glu Lys Arg Met Thr Pro Pro Gly
                405                 410                 415     
Gly Thr Leu Pro Gly Ala Glu Asp Val Tyr Gly Gly Ser Arg Asp Thr
            420                 425                 430         
Pro Pro His His Gly Val Gln Thr Pro Val Gln
        435                 440             


<210> 4
<211> 85
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic human full-length precursor miR-124 microRNA sequence
      hsa-miR-124-1, miRBase Accession No. MI0000443

<400> 4
aggccucucu cuccguguuc acagcggacc uugauuuaaa uguccauaca auuaaggcac 60
gcggugaaug ccaagaaugg ggcug                                       85

<210> 5
<211> 109
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic human full-length precursor miR-124 microRNA sequence
      hsa-miR-124-2, miRBase Accession No. MI0000444

<400> 5
aucaagauua gaggcucugc ucuccguguu cacagcggac cuugauuuaa ugucauacaa 60
uuaaggcacg cggugaaugc caagagcgga gccuacggcu gcacuugaa             109

<210> 6
<211> 87
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic human full-length precursor miR-124 microRNA sequence
      hsa-miR-124-3, miRBase Accession No. MI0000445

<400> 6
ugagggcccc ucugcguguu cacagcggac cuugauuuaa ugucuauaca auuaaggcac 60
gcggugaaug ccaagagagg cgccucc                                     87

<210> 7
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic MYT1L transcription factor DNA-binding
      zinc finger domain

<400> 7
Cys Cys His Cys
 1              


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic RT-PCR primer SOX1 forward

<400> 8
actccagggt acacacaggg                                             20

<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic RT-PCR primer SOX1 reverse

<400> 9
tctgttaact caccgggacc                                             20

<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic RT-PCR primer SOX10 forward

<400> 10
cgcttgtcac tttcgttcag                                             20

<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic RT-PCR primer SOX10 reverse

<400> 11
ccttcatggt gtgggctc                                               18


