LISTADO DE SECUENCIAS

<110>  INSTITUTO CIENTIFICO Y TECNOLOGICO DE NAVARRA S.A.
 
<120>  Mtodo DIVA de diferenciacin de animales vacunados frente a la brucelosis 

<130>  PCT-05156

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  55
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador directo del gen wbdR attB

<400>  1
ggggacaagt ttgtacaaaa aagcaggctt catgaatttg tatggtattt ttggt            55


<210>  2
<211>  52
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador inverso del gen wbdR attB

<400>  2
ggggaccact ttgtacaaga aagctgggtc ttaaatagat gttggcgatc tt               52


<210>  3
<211>  19
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador directo wadB-F1 del gen wadB

<400>  3
gcatgattac cccgctgat                                                    19


<210>  4
<211>  21
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador inverso wadB-R2 del gen wadB

<400>  4
cgcaatctcg tctttgttga g                                                 21
<210>  5
<211>  40
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador directo wadB-F3 del gen wadB

<400>  5
ctcaacaaag acgagattgc gggtggcgtg aaggaaatct                             40


<210>  6
<211>  20
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador inverso wadB-R4 del gen wadB

<400>  6
tgatagccga gcctcttcag                                                   20


<210>  7
<211>  666
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(666)

<400>  7
atg aat ttg tat ggt att ttt ggt gct gga agt tat ggt aga gaa aca         48
Met Asn Leu Tyr Gly Ile Phe Gly Ala Gly Ser Tyr Gly Arg Glu Thr           
1               5                   10                  15                

ata ccc att cta aat caa caa ata aag caa gaa tgt ggt tct gac tat         96
Ile Pro Ile Leu Asn Gln Gln Ile Lys Gln Glu Cys Gly Ser Asp Tyr           
            20                  25                  30                    

gct ctg gtt ttt gtg gat gat gtt ttg gca gga aag aaa gtt aat ggt        144
Ala Leu Val Phe Val Asp Asp Val Leu Ala Gly Lys Lys Val Asn Gly           
        35                  40                  45                        

ttt gaa gtg ctt tca acc aac tgc ttt cta aaa gcc cct tat tta aaa        192
Phe Glu Val Leu Ser Thr Asn Cys Phe Leu Lys Ala Pro Tyr Leu Lys           
    50                  55                  60                            

aag tat ttt aat gtt gct att gct aat gat aag ata cga cag aga gtg        240
Lys Tyr Phe Asn Val Ala Ile Ala Asn Asp Lys Ile Arg Gln Arg Val           
65                  70                  75                  80            

tct gag tca ata tta tta cac ggg gtt gaa cca ata act ata aaa cat        288
Ser Glu Ser Ile Leu Leu His Gly Val Glu Pro Ile Thr Ile Lys His           
                85                  90                  95                

cca aat agc gtt gtt tat gat cat act atg ata ggt agt ggc gct att        336
Pro Asn Ser Val Val Tyr Asp His Thr Met Ile Gly Ser Gly Ala Ile           
            100                 105                 110                   

att tct ccc ttt gtt aca ata tct act aat act cat ata ggg agg ttt        384
Ile Ser Pro Phe Val Thr Ile Ser Thr Asn Thr His Ile Gly Arg Phe           
        115                 120                 125                       

ttt cat gca aac ata tac tca tac gtt gca cat gat tgt caa ata gga        432
Phe His Ala Asn Ile Tyr Ser Tyr Val Ala His Asp Cys Gln Ile Gly           
    130                 135                 140                           

gac tat gtt aca ttt gct cct ggg gct aaa tgt aat gga tat gtt gtt        480
Asp Tyr Val Thr Phe Ala Pro Gly Ala Lys Cys Asn Gly Tyr Val Val           
145                 150                 155                 160           

att gaa gac aat gca tat ata ggc tcg ggt gca gta att aag cag ggt        528
Ile Glu Asp Asn Ala Tyr Ile Gly Ser Gly Ala Val Ile Lys Gln Gly           
                165                 170                 175               

gtt cct aat cgc cca ctt att att ggc gcg gga gcc att ata ggt atg        576
Val Pro Asn Arg Pro Leu Ile Ile Gly Ala Gly Ala Ile Ile Gly Met           
            180                 185                 190                   

ggg gct gtt gtc act aaa agt gtt cct gcc ggt ata act gtg tgc gga        624
Gly Ala Val Val Thr Lys Ser Val Pro Ala Gly Ile Thr Val Cys Gly           
        195                 200                 205                       

aat cca gca aga gaa atg aaa aga tcg cca aca tct att taa                666
Asn Pro Ala Arg Glu Met Lys Arg Ser Pro Thr Ser Ile                       
    210                 215                 220                           


<210>  8
<211>  221
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Asn Leu Tyr Gly Ile Phe Gly Ala Gly Ser Tyr Gly Arg Glu Thr 
1               5                   10                  15      


Ile Pro Ile Leu Asn Gln Gln Ile Lys Gln Glu Cys Gly Ser Asp Tyr 
            20                  25                  30          


Ala Leu Val Phe Val Asp Asp Val Leu Ala Gly Lys Lys Val Asn Gly 
        35                  40                  45              


Phe Glu Val Leu Ser Thr Asn Cys Phe Leu Lys Ala Pro Tyr Leu Lys 
    50                  55                  60                  


Lys Tyr Phe Asn Val Ala Ile Ala Asn Asp Lys Ile Arg Gln Arg Val 
65                  70                  75                  80  


Ser Glu Ser Ile Leu Leu His Gly Val Glu Pro Ile Thr Ile Lys His 
                85                  90                  95      


Pro Asn Ser Val Val Tyr Asp His Thr Met Ile Gly Ser Gly Ala Ile 
            100                 105                 110         


Ile Ser Pro Phe Val Thr Ile Ser Thr Asn Thr His Ile Gly Arg Phe 
        115                 120                 125             


Phe His Ala Asn Ile Tyr Ser Tyr Val Ala His Asp Cys Gln Ile Gly 
    130                 135                 140                 


Asp Tyr Val Thr Phe Ala Pro Gly Ala Lys Cys Asn Gly Tyr Val Val 
145                 150                 155                 160 


Ile Glu Asp Asn Ala Tyr Ile Gly Ser Gly Ala Val Ile Lys Gln Gly 
                165                 170                 175     


Val Pro Asn Arg Pro Leu Ile Ile Gly Ala Gly Ala Ile Ile Gly Met 
            180                 185                 190         


Gly Ala Val Val Thr Lys Ser Val Pro Ala Gly Ile Thr Val Cys Gly 
        195                 200                 205             


Asn Pro Ala Arg Glu Met Lys Arg Ser Pro Thr Ser Ile 
    210                 215                 220     


<210>  9
<211>  24
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>   Cebador directo del gen wbdR

<400>  9
atgaatttgt atggtatttt tggt                                              24


<210>  10
<211>  22
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador inverso del gen wbdR

<400>  10
ttaaatagat gttggcgatc tt                                                22


<210>  11
<211>  20
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador directo del gen wbdR

<400>  11
tgatgttttg gcaggaaaga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador inverso del gen wbdR

<400>  12
tggatttccg cacacagtta                                                   20


<210>  13
<211>  1083
<212>  DNA
<213>  Brucella abortus N Acceso YP_413819.1 (Gene ID:3787122)


<220>
<221>  caracterstica
<222>  (29)..(47)
<223>  Cebador directo wadb-F1 del gen wadB (SEQ ID NO:3)

<220>
<221>  codn terminacin
<222>  (49)..(51)
<223>  Codn terminacin BAB1_0352

<220>
<221>  caracterstica
<222>  (52)..(180)
<223>  regin intergnica entre BAB1_0352 y BAB1_0351

<220>
<221>  codn iniciacin
<222>  (181)..(183)
<223>  Codn iniciacin BAB1_0351 (wadb)

<220>
<221>  CDS
<222>  (181)..(900)

<220>
<221>  caracterstica
<222>  (304)..(324)
<223>  Cebador inverso wadB-R2 del gen wadB (SEQ ID NO: 4)

<220>
<221>  mutacin
<222>  (325)..(763)
<223>  zona delecionada en el mutante BAB-wadB

<220>
<221>  caracterstica
<222>  (683)..(704)+(766)..(784)
<223>  Cebador directo wadB-F3 del gen wadB (SEQ ID NO:5)

<220>
<221>  codn terminacin
<222>  (898)..(900)
<223>  Codn terminacin BAB1_0351 (wadB)

<220>
<221>  caracterstica
<222>  (901)..(908)
<223>  regin intergnica entre BAB1_0351 y BAB1_0350

<220>
<221>  codn iniciacin
<222>  (909)..(911)
<223>  Codn iniciacin BAB1_0350

<220>
<221>  caracterstica
<222>  (1018)..(1037)
<223>  Cebador directo wadB-R4 del gen wadB (SEQ ID NO:5)

<400>  13
cagtggatcg aggaaaaaat caaatcgggc atgattaccc cgctgatctg agccggtaat       60

tataccggcc cttatgcctc tctccggtgg atttcggtcg ttttgcatgc catttgtcga      120

aatgccaaat ttcgtgcata taacgcgctt tcctgaatca cagccatcgg tcccgcgtcc      180

atg aac gtc aag gca ttc atc att cac ctc aaa cgc gcg acc gat cgt        228
Met Asn Val Lys Ala Phe Ile Ile His Leu Lys Arg Ala Thr Asp Arg           
1               5                   10                  15                

gcg ccg cag gtg gaa aaa ctc atc aag gaa ttg ccg gtc aag gcc gaa        276
Ala Pro Gln Val Glu Lys Leu Ile Lys Glu Leu Pro Val Lys Ala Glu           
            20                  25                  30                    

gtc atc gaa gcc gtc gat agc cgt gcg ctc aac aaa gac gag att gcg        324
Val Ile Glu Ala Val Asp Ser Arg Ala Leu Asn Lys Asp Glu Ile Ala           
        35                  40                  45                        

cgc att tat aaa cgc cgc ctg cac act ccg cgc tat ccc ttt gcc ctc        372
Arg Ile Tyr Lys Arg Arg Leu His Thr Pro Arg Tyr Pro Phe Ala Leu           
    50                  55                  60                            

agc cgc aac gag att gcc tgt ttc ctg tcg cac cgc aag gca tgg cag        420
Ser Arg Asn Glu Ile Ala Cys Phe Leu Ser His Arg Lys Ala Trp Gln           
65                  70                  75                  80            

gcg att atc gac cgg aag ctt gat gcg ggc ttc atc gtg gaa gac gat        468
Ala Ile Ile Asp Arg Lys Leu Asp Ala Gly Phe Ile Val Glu Asp Asp           
                85                  90                  95                

att gca ctg acg gaa aac ttc atg ggt gca tac cgg gcc gcg gtc gat        516
Ile Ala Leu Thr Glu Asn Phe Met Gly Ala Tyr Arg Ala Ala Val Asp           
            100                 105                 110                   

cat ctg gaa ccg ggt ggc ttc atc cgc ttt aca ttc cgg gac gac cgc        564
His Leu Glu Pro Gly Gly Phe Ile Arg Phe Thr Phe Arg Asp Asp Arg           
        115                 120                 125                       

gag cat ggc cgt gag gtt ttc cgg gac gaa gcg gtg cgg atc atc atc        612
Glu His Gly Arg Glu Val Phe Arg Asp Glu Ala Val Arg Ile Ile Ile           
    130                 135                 140                           

ccg aac ccg atc ggc ctt ggc atg gtt gcg cag ttt gtt tcc tat gat        660
Pro Asn Pro Ile Gly Leu Gly Met Val Ala Gln Phe Val Ser Tyr Asp           
145                 150                 155                 160           

gcg gcc caa aaa ctg ctc gac atc acc cag acg ttc gac cgg cct gtc        708
Ala Ala Gln Lys Leu Leu Asp Ile Thr Gln Thr Phe Asp Arg Pro Val           
                165                 170                 175               

gat acg acg gtg cag atg cgc tgg gta acg ggc ctg caa ccc ctt tcg        756
Asp Thr Thr Val Gln Met Arg Trp Val Thr Gly Leu Gln Pro Leu Ser           
            180                 185                 190                   

gtc att ccg ggt ggc gtg aag gaa atc tcg tca caa ctg ggc gga acc        804
Val Ile Pro Gly Gly Val Lys Glu Ile Ser Ser Gln Leu Gly Gly Thr           
        195                 200                 205                       

acg atc cag cac aaa aag aac ttt tca gac aag ctt gcg cgc gaa att        852
Thr Ile Gln His Lys Lys Asn Phe Ser Asp Lys Leu Ala Arg Glu Ile           
    210                 215                 220                           

tta cgc ccc atc tac cgg atg cgt gtg cgc gcc tat tca tcg aaa tga        900
Leu Arg Pro Ile Tyr Arg Met Arg Val Arg Ala Tyr Ser Ser Lys               
225                 230                 235                               

gttttatgat gcctgtacct attcttttgt atcaccagat cgcgccatta ccggccaaaa      960

acattccatt ccgcggcttg ctggttcatc ctgaccgctt ccgcagccag atgcgctggc     1020

tgaagaggct cggctatcaa ggtctctcgc tgcgcgatgc catgccctat atcaagggtg     1080

aaa                                                                   1083


<210>  14
<211>  239
<212>  PRT
<213>  Brucella abortus N Acceso YP_413819.1 (Gen ID:3787122)

<400>  14

Met Asn Val Lys Ala Phe Ile Ile His Leu Lys Arg Ala Thr Asp Arg 
1               5                   10                  15      


Ala Pro Gln Val Glu Lys Leu Ile Lys Glu Leu Pro Val Lys Ala Glu 
            20                  25                  30          


Val Ile Glu Ala Val Asp Ser Arg Ala Leu Asn Lys Asp Glu Ile Ala 
        35                  40                  45              


Arg Ile Tyr Lys Arg Arg Leu His Thr Pro Arg Tyr Pro Phe Ala Leu 
    50                  55                  60                  


Ser Arg Asn Glu Ile Ala Cys Phe Leu Ser His Arg Lys Ala Trp Gln 
65                  70                  75                  80  


Ala Ile Ile Asp Arg Lys Leu Asp Ala Gly Phe Ile Val Glu Asp Asp 
                85                  90                  95      


Ile Ala Leu Thr Glu Asn Phe Met Gly Ala Tyr Arg Ala Ala Val Asp 
            100                 105                 110         


His Leu Glu Pro Gly Gly Phe Ile Arg Phe Thr Phe Arg Asp Asp Arg 
        115                 120                 125             


Glu His Gly Arg Glu Val Phe Arg Asp Glu Ala Val Arg Ile Ile Ile 
    130                 135                 140                 


Pro Asn Pro Ile Gly Leu Gly Met Val Ala Gln Phe Val Ser Tyr Asp 
145                 150                 155                 160 


Ala Ala Gln Lys Leu Leu Asp Ile Thr Gln Thr Phe Asp Arg Pro Val 
                165                 170                 175     


Asp Thr Thr Val Gln Met Arg Trp Val Thr Gly Leu Gln Pro Leu Ser 
            180                 185                 190         


Val Ile Pro Gly Gly Val Lys Glu Ile Ser Ser Gln Leu Gly Gly Thr 
        195                 200                 205             


Thr Ile Gln His Lys Lys Asn Phe Ser Asp Lys Leu Ala Arg Glu Ile 
    210                 215                 220                 


Leu Arg Pro Ile Tyr Arg Met Arg Val Arg Ala Tyr Ser Ser Lys 
225                 230                 235                






