                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Inc.
 
<120>  Over-Expression of NADH-Dependent Oxidoreductase (FUCO) for 
       Increasing Furfural or 5-Hydroxymethylfurfural Tolerance

<130>  UF.972XPCT

<150>  US 61/470,642
<151>  2011-04-01

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  382
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ala Asn Arg Met Ile Leu Asn Glu Thr Ala Trp Phe Gly Arg Gly 
1               5                   10                  15      


Ala Val Gly Ala Leu Thr Asp Glu Val Lys Arg Arg Gly Tyr Gln Lys 
            20                  25                  30          


Ala Leu Ile Val Thr Asp Lys Thr Leu Val Gln Cys Gly Val Val Ala 
        35                  40                  45              


Lys Val Thr Asp Lys Met Asp Ala Ala Gly Leu Ala Trp Ala Ile Tyr 
    50                  55                  60                  


Asp Gly Val Val Pro Asn Pro Thr Ile Thr Val Val Lys Glu Gly Leu 
65                  70                  75                  80  


Gly Val Phe Gln Asn Ser Gly Ala Asp Tyr Leu Ile Ala Ile Gly Gly 
                85                  90                  95      


Gly Ser Pro Gln Asp Thr Cys Lys Ala Ile Gly Ile Ile Ser Asn Asn 
            100                 105                 110         


Pro Glu Phe Ala Asp Val Arg Ser Leu Glu Gly Leu Ser Pro Thr Asn 
        115                 120                 125             


Lys Pro Ser Val Pro Ile Leu Ala Ile Pro Thr Thr Ala Gly Thr Ala 
    130                 135                 140                 


Ala Glu Val Thr Ile Asn Tyr Val Ile Thr Asp Glu Glu Lys Arg Arg 
145                 150                 155                 160 


Lys Phe Val Cys Val Asp Pro His Asp Ile Pro Gln Val Ala Phe Ile 
                165                 170                 175     


Asp Ala Asp Met Met Asp Gly Met Pro Pro Ala Leu Lys Ala Ala Thr 
            180                 185                 190         


Gly Val Asp Ala Leu Thr His Ala Ile Glu Gly Tyr Ile Thr Arg Gly 
        195                 200                 205             


Ala Trp Ala Leu Thr Asp Ala Leu His Ile Lys Ala Ile Glu Ile Ile 
    210                 215                 220                 


Ala Gly Ala Leu Arg Gly Ser Val Ala Gly Asp Lys Asp Ala Gly Glu 
225                 230                 235                 240 


Glu Met Ala Leu Gly Gln Tyr Val Ala Gly Met Gly Phe Ser Asn Val 
                245                 250                 255     


Gly Leu Gly Leu Val His Gly Met Ala His Pro Leu Gly Ala Phe Tyr 
            260                 265                 270         


Asn Thr Pro His Gly Val Ala Asn Ala Ile Leu Leu Pro His Val Met 
        275                 280                 285             


Arg Tyr Asn Ala Asp Phe Thr Gly Glu Lys Tyr Arg Asp Ile Ala Arg 
    290                 295                 300                 


Val Met Gly Val Lys Val Glu Gly Met Ser Leu Glu Glu Ala Arg Asn 
305                 310                 315                 320 


Ala Ala Val Glu Ala Val Phe Ala Leu Asn Arg Asp Val Gly Ile Pro 
                325                 330                 335     


Pro His Leu Arg Asp Val Gly Val Arg Lys Glu Asp Ile Pro Ala Leu 
            340                 345                 350         


Ala Gln Ala Ala Leu Asp Asp Val Cys Thr Gly Gly Asn Pro Arg Glu 
        355                 360                 365             


Ala Thr Leu Glu Asp Ile Val Glu Leu Tyr His Thr Ala Trp 
    370                 375                 380         


<210>  2
<211>  1149
<212>  DNA
<213>  Escherichia coli

<400>  2
ttaccaggcg gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc       60

accggtacaa acatcatcca gtgccgcctg agccagtgcc ggaatgtctt ccttgcgtac      120

accaacatca cgcaaatgtg gcggaatacc gacatcacgg ttgagagcaa acaccgcttc      180

aacagcggca ttacgcgcct cttccaggct cataccttcc actttcacgc ccataacgcg      240

cgcgatatcg cggtacttct caccggtaaa gtcagcgtta tagcgcatga catgcggtaa      300

caggatggcg ttcgcaacac cgtgtggagt gttgtaaaac gcgcccagtg gatgcgccat      360

accatgcacc aaccctaacc caacattcga gaagcccata cccgcaacat actgcccgag      420

cgccatttct tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat      480

gatttcaatc gctttaatgt gcagtgcatc ggttagcgcc cacgcgccac gggtaatata      540

cccctcaata gcatgagtga gcgcatcgac acccgtcgca gctttcagcg ctggaggcat      600

accatccatc atgtcagcgt caataaacgc cacctgcggg atatcatgcg gatcaacgca      660

aacaaacttg cgccgttttt cttcgtcagt gatcacgtag ttaatggtca cttctgccgc      720

agtgcctgct gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag      780

tccttccagg ctacgcacat cggcaaactc cgggttgttg ctgataatgc caatcgcttt      840

acaagtatcc tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg      900

gaatacaccg agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta      960

aatcgcccat gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca     1020

ttgcaccagc gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc     1080

atcggttaaa gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct     1140

gttagccat                                                             1149


<210>  3
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  fucO for EcoRI primer

<400>  3
cgcgcggaat tcgattgccg tagtgctgga ga                                     32


<210>  4
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  fucO rev BamHI primer

<400>  4
cgcgcgggat cctgcggttg gtacggtaac gg                                     32


<210>  5
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldhA forward primer

<400>  5
gataacggag atcgggaatg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldhA reverse primer

<400>  6
ctttggctgt cagttcacca                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldha-1 primer

<400>  7
tctggaaaaa ggcgaaacct                                                   20


<210>  8
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldha-2 primer

<400>  8
tttgtgctat aaacggcgag t                                                 21


<210>  9
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldhL ORF up primer

<400>  9
atgtctaata ttcaaaatca tcaaaaagtt gtcctcgtcg                             40


<210>  10
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldhL ORF down primer

<400>  10
ttatttgtct tgtttttcag caagagcgtt tagac                                  35


<210>  11
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldhA reverse1 primer

<400>  11
aagactttct ccagtgatgt tgaatcac                                          28


<210>  12
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ldhA forward1 primer

<400>  12
tcttgccgct cccct                                                        15


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pdc forward primer

<400>  13
tggtctcaag catcacttcg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhB reverse primer

<400>  14
ttggtcagag cacaagcatc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhB-1 primer

<400>  15
cccacgcatt tgaagcttat                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pdc-2 primer

<400>  16
atcgatttta gccggagctt                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  estZ forward primer

<400>  17
actggcatct gagttctctg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  estZ reverse primer

<400>  18
ttccatggcg tgagttactg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  estZ-1 primer

<400>  19
cagaccgtgc ggaatatgga                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  estZ-2 primer

<400>  20
cagcctcgat tcgcatgaca                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE forward primer

<400>  21
caatacgcct tttgacagca                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE reverse primer

<400>  22
gccatcaatg gcaaaaagtt                                                   20


<210>  23
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE-1 primer

<400>  23
tcagtagcgc tgtctggca                                                    19


<210>  24
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE-2 primer

<400>  24
aatgctctcc tgataatgtt aaactttttt agta                                   34


<210>  25
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  yqhD forward primer

<400>  25
tatgatgcca ggctcgtaca                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  yqhD reverse primer

<400>  26
gatcatgcct ttccatgctt                                                   20


<210>  27
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  yqhD-1 primer

<400>  27
gctttttacg cctcaaactt tcgt                                              24


<210>  28
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  yqhD-2 primer

<400>  28
tacttgctcc ctttgctgg                                                    19


<210>  29
<211>  61
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE-fucO ORF forward primer

<400>  29
aatgctctcc tgataatgtt aaactttttt agtaatgatg gctaacagaa tgattctgaa       60

c                                                                       61


<210>  30
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE-fucO ORF reverse primer

<400>  30
tgccagacag cgctactgat taccaggcgg tatggtaaag                             40


<210>  31
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE-fucO-1 primer

<400>  31
ctttaccata ccgcctggta atcagtagcg ctgtctggca                             40


<210>  32
<211>  61
<212>  DNA
<213>  Artificial sequence

<220>
<223>  adhE-fucO-2 primer

<400>  32
gttcagaatc attctgttag ccatcattac taaaaaagtt taacattatc aggagagcat       60

t                                                                       61


