                         SEQUENCE LISTING

<110>  Codexis, Inc.
       WONG, Brian
       SUKUMARAN, Joly
       YEO, Wan Lin
       GOHEL, Anupam
       SMITH, Derek
       COLLIER, Steven J.
 
<120>  BIOCATALYTIC PROCESS FOR PREPARING ESLICARBAZEPINE AND ANALOGS 
       THEREOF

<130>  CX2-085WO1

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized L. kefir ketoreductase gene

<400>  1
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  2
<211>  252
<212>  PRT
<213>  Lactobacillus kefir

<400>  2

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  3
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  3
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttgttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggtt cgtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgctgctcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtggacc gcacagtga                             759


<210>  4
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  4

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Phe Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Leu Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 
                245                 250         


<210>  5
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  5
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttgttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggtt cgtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgctgctcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtggacc gcacagtga                             759


<210>  6
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  6

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Phe Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Leu Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 
                245                 250         


<210>  7
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  7
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttgttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgctgctcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtggacc gcacagtga                             759


<210>  8
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  8

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Leu Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln 
                245                 250         


<210>  9
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  9
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttgttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggtt cgtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgctgctcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  10
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  10

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Phe Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Leu Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  11
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  11
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttgttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggtt cgtaggctta ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgctgatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  12
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  12

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Phe Val Gly Leu Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  13
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  13
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg ccgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg ttctggggtt cgtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  14
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  14

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Leu Gly Phe Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  15
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  15
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccgacgactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  16
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  16

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  17
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  17
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gcatggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg ttctggggca ggtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggttcacc gcacagtga                             759


<210>  18
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  18

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Met Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Phe Thr Ala Gln 
                245                 250         


<210>  19
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  19
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gcatggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg ttctggggtt cgtaggcgat ccgacgacgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttactgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  20
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  20

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Met Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Leu Gly Phe Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Leu Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  21
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  21
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaat gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg cgtccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagtg ttctggggat ggtaggcttg ccgaccactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc gtttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  22
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  22

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Val 
    130                 135                 140                 


Leu Gly Met Val Gly Leu Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Arg Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  23
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  23
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatcgcg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ccaaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccgacgactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  24
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  24

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Arg Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  25
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  25
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatcgcg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg acgaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccgacgactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctggcg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  26
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  26

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Arg Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Gly Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  27
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  27
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcgc      120

gcggatcgcg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ccaaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gcgtggttaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccgacgactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  28
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  28

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Arg Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Thr Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  29
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  29
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtaacagag ggtgcgaaag tagttattac tggtcgtcgc      120

gcggatcgcg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ccaaaactgt tcgacgccac cgaggagaca      240

ttcggcccgg tcacgaccct ggtgaacaat gcagggattg gctatcgcaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccgttaactg gggcatactg tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatgccg      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  30
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  30

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Thr Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Arg Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Ala Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Tyr Arg 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Leu Thr Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Pro Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  31
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  31
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtaacagag ggtgcgaaag tagttattac tggtcgtcgc      120

gcggatcgcg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ccaaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gctatcgcaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa tgtaaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccggcgactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  32
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  32

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Thr Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Arg Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Tyr Arg 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Cys Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Ala Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  33
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  33
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacact gggtatcggt       60

ttggcaatcg ccgataaatt tgtaacagag ggtgcgaaag tagttattac tggtcgtcgc      120

gcggatgttg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ccaaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gctatcgcaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa tgtaaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccggcgactg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatgccg      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  34
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  34

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Thr Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Tyr Arg 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Cys Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Ala Thr Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Pro Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  35
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  35
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca cggtatcggt       60

ttggcaatcg ccactaaatt tgtaacagag ggtgcgaaag tagttattac tggtcgtcgc      120

gcggatgttg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ggcaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gctatcgcaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa tgtaaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccggcgactg gggcatactc cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatgccg      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  36
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  36

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


His Gly Ile Gly Leu Ala Ile Ala Thr Lys Phe Val Thr Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Gly Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Tyr Arg 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Cys Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Ala Thr Gly Ala Tyr Ser Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Pro Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  37
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  37
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggacaca cggtatcggt       60

ttggcaatcg ccgataaatt tgtaacagag ggtgcgaaag tagttattac tggtcgtcgc      120

gcggatgttg gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg tttccgatga agcaggctgg ccaaaactgt tcgacaccac cgaggagaca      240

ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gctatcgcaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa tgtaaaggct tgggcgctag catcatcaat      420

atgagcagta ttctggggca ggtaggcgat ccggcgactg gggcatactc cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctgctgt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgttaatgga tgatatgccg      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggtcacat tggcgaaccg      660

aatgacatcg catgggtctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  38
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of L. kefir ketoreductase

<400>  38

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


His Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Thr Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg Arg Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Val 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Pro Lys Leu Phe Asp Thr Thr Glu Glu Thr 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Tyr Arg 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Cys Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Leu Gly Gln Val Gly Asp Pro Ala Thr Gly Ala Tyr Ser Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Leu Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         


Thr Pro Leu Met Asp Asp Met Pro Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


