                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  NOVEL BACTERIAL EXPRESSION PLASMID

<130>  p10467-03

<150>  61/475,775
<151>  2011-04-15

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insert sequence for pPGtrpRop63 comprising c-terminal portion of 
       Rop protein, purification tag sequence, protease cleavage site, 
       and multiple cloning site

<400>  1

Leu Tyr Arg Ser Cys Leu Ala Arg Phe Gly Asp Asp Gly Glu Asn Leu 
1               5                   10                  15      


Arg Ser His His His His His His Arg Ser Leu Val Pro Arg Gly Thr 
            20                  25                  30          


Pro Gly Ile Ser Ala Asp Leu Asp 
        35                  40  


<210>  2
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Insert sequence for pPGtrpRop63 encoding c-terminal portion of 
       Rop protein, purification tag sequence, protease cleavage site, 
       and multiple cloning site

<400>  2
ctttaccgca gttgtctcgc acgtttcggg gatgacggtg aaaacctcag atcccaccat       60

caccatcacc atagatctct ggttccgcgt ggtacccccg ggatatcagc tgatctagat      120

tagtatctgc ctcgc                                                       135


<210>  3
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modification of KpnI and PvuII site to produce SmaI and BamHI 
       site

<400>  3
ccccggggga tccag                                                        15


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modification of KpnI and PvuII site to produce SmaI and BamHI 
       site

<400>  4
catgggggcc ccctaggtc                                                    19


<210>  5
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modification of KpnI and PvuII site to produce SmaI and BamHI 
       site

<400>  5
ccccggggat ccag                                                         14


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modification of KpnI and PvuII site to produce SmaI and BamHI 
       site

<400>  6
catgggggcc cctaggtc                                                     18


<210>  7
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modification of KpnI and PvuII site to produce SmaI and BamHI 
       site

<400>  7
ccccgggatc cag                                                          13


<210>  8
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modification of KpnI and PvuII site to produce SmaI and BamHI 
       site

<400>  8
catgggggcc ctaggtc                                                      17


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thrombin cleavage site

<400>  9

Leu Val Pro Arg Gly Thr 
1               5       


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' primer used to generate a PCR product from pcDNA3.1-myc-His 
       that encodes the entire murine Survivin gene with XmaI 
       restriction site termini

<400>  10
catcccggga tgggagctcc ggcgctgccc                                        30


<210>  11
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' primer used to generate a PCR product from pcDNA3.1-myc-His 
       that encodes the entire murine Survivin gene with XmaI 
       restriction site termini

<400>  11
taccccgggt taggcagcca gctgctcaat tga                                    33


<210>  12
<211>  63
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(63)
<223>  Amino acid sequence of regulatory protein Rop from E. Coli

<400>  12

Met Thr Lys Gln Glu Lys Thr Ala Leu Asn Met Ala Arg Phe Ile Arg 
1               5                   10                  15      


Ser Gln Thr Leu Thr Leu Leu Glu Lys Leu Asn Glu Leu Asp Ala Asp 
            20                  25                  30          


Glu Gln Ala Asp Ile Cys Glu Ser Leu His Asp His Ala Asp Glu Leu 
        35                  40                  45              


Tyr Arg Ser Cys Leu Ala Arg Phe Gly Asp Asp Gly Glu Asn Leu 
    50                  55                  60              


