                         SEQUENCE LISTING



<110>  The United States of America, as represented by the 

       Secretary, Department of Health and Human Services

       Chiorini, John A.

       Di Pasquale, Giovanni

 

<120>  AAV MEDIATED EXENDIN-4 GENE TRANSFER TO SALILVARY GLANDS TO 

       PROTECT SUBJECTS FROM DIABETES OR OBESITY



<130>  6137NIDCR-13-PROV



<160>  10    



<170>  PatentIn version 3.5



<210>  1

<211>  900

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Synthetic





<220>

<221>  CDS

<222>  (214)..(699)



<400>  1

catgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc       60



aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc      120



gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct      180



ggtttagtga accgtcagat ccgctagccc acc atg tcc atg ttg ttc tac act       234

                                     Met Ser Met Leu Phe Tyr Thr          

                                     1               5                    



ctg atc act gcg ttt ttg atc ggc gta cag gca gaa ccg tac aca gat        282

Leu Ile Thr Ala Phe Leu Ile Gly Val Gln Ala Glu Pro Tyr Thr Asp           

        10                  15                  20                        



agc aat gtc cca gaa gga gac tct gtc cct gaa gcc cac tgg act aaa        330

Ser Asn Val Pro Glu Gly Asp Ser Val Pro Glu Ala His Trp Thr Lys           

    25                  30                  35                            



ctt cag cat tcc ctt gac aca gcc ctc cgc aga gcc cgc agt gcc cct        378

Leu Gln His Ser Leu Asp Thr Ala Leu Arg Arg Ala Arg Ser Ala Pro           

40                  45                  50                  55            



act gca cca ata gct gcc cga gtg aca ggg cag acc cgc aac atc act        426

Thr Ala Pro Ile Ala Ala Arg Val Thr Gly Gln Thr Arg Asn Ile Thr           

                60                  65                  70                



gta gac ccc aga ctg ttt aag aaa cgg aga ctc cac tca ccc cgt gtg        474

Val Asp Pro Arg Leu Phe Lys Lys Arg Arg Leu His Ser Pro Arg Val           

            75                  80                  85                    



ctg ttc agc acc cag cct cca ccc acc tct tca gac act ctg gat cta        522

Leu Phe Ser Thr Gln Pro Pro Pro Thr Ser Ser Asp Thr Leu Asp Leu           

        90                  95                  100                       



gac ttc cag gcc cac ggt aca atc cct ttc aac agg act cac cgg agc        570

Asp Phe Gln Ala His Gly Thr Ile Pro Phe Asn Arg Thr His Arg Ser           

    105                 110                 115                           



aag cgc cat ggt gaa gga aca ttt acc agt gac ttg tca aaa cag atg        618

Lys Arg His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met           

120                 125                 130                 135           



gaa gag gag gca gtg cgg tta ttt att gag tgg ctt aag aac gga gga        666

Glu Glu Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly           

                140                 145                 150               



cca agt agc ggg gca cct ccg cca tcg ggt taa ggatcccggg gccgtcttcc      719

Pro Ser Ser Gly Ala Pro Pro Pro Ser Gly                                   

            155                 160                                       



cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt gggcatgggt cagaaggatt      779



cctatgtggg cgacgaggcc cagagcaaga gaggcatcct caccctgaag taccccatcg      839



agcacggcat cgtcaccaac tgggacgaca tggagaaaat ctggcaccac accttctaca      899



a                                                                      900





<210>  2

<211>  161

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Synthetic Construct



<400>  2



Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Val 

1               5                   10                  15      





Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly Asp Ser Val 

            20                  25                  30          





Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu 

        35                  40                  45              





Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala Arg Val Thr 

    50                  55                  60                  





Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg 

65                  70                  75                  80  





Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Pro Thr 

                85                  90                  95      





Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly Thr Ile Pro 

            100                 105                 110         





Phe Asn Arg Thr His Arg Ser Lys Arg His Gly Glu Gly Thr Phe Thr 

        115                 120                 125             





Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg Leu Phe Ile 

    130                 135                 140                 





Glu Trp Leu Lys Asn Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser 

145                 150                 155                 160 





Gly 

    





<210>  3

<211>  503

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Synthetic





<220>

<221>  CDS

<222>  (11)..(493)



<400>  3

ctagcccacc atg tcc atg ttg ttc tac act ctg atc act gcg ttt ttg          49

           Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu            

           1               5                   10                         



atc ggc gta cag gca gaa ccg tac aca gat agc aat gtc cca gaa gga         97

Ile Gly Val Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly           

    15                  20                  25                            



gac tct gtc cct gaa gcc cac tgg act aaa ctt cag cat tcc ctt gac        145

Asp Ser Val Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp           

30                  35                  40                  45            



aca gcc ctc cgc aga gcc cgc agt gcc cct act gca cca ata gct gcc        193

Thr Ala Leu Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala           

                50                  55                  60                



cga gtg aca ggg cag acc cgc aac atc act gta gac ccc aga ctg ttt        241

Arg Val Thr Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe           

            65                  70                  75                    



aag aaa cgg aga ctc cac tca ccc cgt gtg ctg ttc agc acc cag cct        289

Lys Lys Arg Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro           

        80                  85                  90                        



cca ccc acc tct tca gac act ctg gat cta gac ttc cag gcc cac ggt        337

Pro Pro Thr Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly           

    95                  100                 105                           



aca atc cct ttc aac agg act cac cgg agc aag cgc cat ggt gaa gga        385

Thr Ile Pro Phe Asn Arg Thr His Arg Ser Lys Arg His Gly Glu Gly           

110                 115                 120                 125           



aca ttt acc agt gac ttg tca aaa cag atg gaa gag gag gca gtg cgg        433

Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg           

                130                 135                 140               



tta ttt att gag tgg ctt aag aac gga gga cca agt agc ggg gca cct        481

Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser Ser Gly Ala Pro           

            145                 150                 155                   



ccg cca tcg ggt taaggatccc                                             503

Pro Pro Ser Gly                                                           

        160                                                               





<210>  4

<211>  161

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Synthetic Construct



<400>  4



Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Val 

1               5                   10                  15      





Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly Asp Ser Val 

            20                  25                  30          





Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu 

        35                  40                  45              





Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala Arg Val Thr 

    50                  55                  60                  





Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg 

65                  70                  75                  80  





Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Pro Thr 

                85                  90                  95      





Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly Thr Ile Pro 

            100                 105                 110         





Phe Asn Arg Thr His Arg Ser Lys Arg His Gly Glu Gly Thr Phe Thr 

        115                 120                 125             





Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg Leu Phe Ile 

    130                 135                 140                 





Glu Trp Leu Lys Asn Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser 

145                 150                 155                 160 





Gly 

    





<210>  5

<211>  483

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Synthetic





<220>

<221>  CDS

<222>  (1)..(483)



<400>  5

atg tcc atg ttg ttc tac act ctg atc act gcg ttt ttg atc ggc gta         48

Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Val           

1               5                   10                  15                



cag gca gaa ccg tac aca gat agc aat gtc cca gaa gga gac tct gtc         96

Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly Asp Ser Val           

            20                  25                  30                    



cct gaa gcc cac tgg act aaa ctt cag cat tcc ctt gac aca gcc ctc        144

Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu           

        35                  40                  45                        



cgc aga gcc cgc agt gcc cct act gca cca ata gct gcc cga gtg aca        192

Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala Arg Val Thr           

    50                  55                  60                            



ggg cag acc cgc aac atc act gta gac ccc aga ctg ttt aag aaa cgg        240

Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg           

65                  70                  75                  80            



aga ctc cac tca ccc cgt gtg ctg ttc agc acc cag cct cca ccc acc        288

Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Pro Thr           

                85                  90                  95                



tct tca gac act ctg gat cta gac ttc cag gcc cac ggt aca atc cct        336

Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly Thr Ile Pro           

            100                 105                 110                   



ttc aac agg act cac cgg agc aag cgc cat ggt gaa gga aca ttt acc        384

Phe Asn Arg Thr His Arg Ser Lys Arg His Gly Glu Gly Thr Phe Thr           

        115                 120                 125                       



agt gac ttg tca aaa cag atg gaa gag gag gca gtg cgg tta ttt att        432

Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg Leu Phe Ile           

    130                 135                 140                           



gag tgg ctt aag aac gga gga cca agt agc ggg gca cct ccg cca tcg        480

Glu Trp Leu Lys Asn Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser           

145                 150                 155                 160           



ggt                                                                    483

Gly                                                                       

                                                                          





<210>  6

<211>  161

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Synthetic Construct



<400>  6



Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Val 

1               5                   10                  15      





Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly Asp Ser Val 

            20                  25                  30          





Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu 

        35                  40                  45              





Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala Arg Val Thr 

    50                  55                  60                  





Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg 

65                  70                  75                  80  





Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Pro Thr 

                85                  90                  95      





Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly Thr Ile Pro 

            100                 105                 110         





Phe Asn Arg Thr His Arg Ser Lys Arg His Gly Glu Gly Thr Phe Thr 

        115                 120                 125             





Ser Asp Leu Ser Lys Gln Met Glu Glu Glu Ala Val Arg Leu Phe Ile 

    130                 135                 140                 





Glu Trp Leu Lys Asn Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser 

145                 150                 155                 160 





Gly 

    





<210>  7

<211>  120

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Synthetic





<220>

<221>  CDS

<222>  (1)..(120)



<400>  7

cat ggt gaa gga aca ttt acc agt gac ttg tca aaa cag atg gaa gag         48

His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu           

1               5                   10                  15                



gag gca gtg cgg tta ttt att gag tgg ctt aag aac gga gga cca agt         96

Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser           

            20                  25                  30                    



agc ggg gca cct ccg cca tcg ggt                                        120

Ser Gly Ala Pro Pro Pro Ser Gly                                           

        35                  40                                            





<210>  8

<211>  40

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Synthetic Construct



<400>  8



His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu 

1               5                   10                  15      





Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser 

            20                  25                  30          





Ser Gly Ala Pro Pro Pro Ser Gly 

        35                  40  





<210>  9

<211>  363

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Synthetic





<220>

<221>  CDS

<222>  (1)..(363)



<400>  9

atg tcc atg ttg ttc tac act ctg atc act gcg ttt ttg atc ggc gta         48

Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Val           

1               5                   10                  15                



cag gca gaa ccg tac aca gat agc aat gtc cca gaa gga gac tct gtc         96

Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly Asp Ser Val           

            20                  25                  30                    



cct gaa gcc cac tgg act aaa ctt cag cat tcc ctt gac aca gcc ctc        144

Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu           

        35                  40                  45                        



cgc aga gcc cgc agt gcc cct act gca cca ata gct gcc cga gtg aca        192

Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala Arg Val Thr           

    50                  55                  60                            



ggg cag acc cgc aac atc act gta gac ccc aga ctg ttt aag aaa cgg        240

Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg           

65                  70                  75                  80            



aga ctc cac tca ccc cgt gtg ctg ttc agc acc cag cct cca ccc acc        288

Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Pro Thr           

                85                  90                  95                



tct tca gac act ctg gat cta gac ttc cag gcc cac ggt aca atc cct        336

Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly Thr Ile Pro           

            100                 105                 110                   



ttc aac agg act cac cgg agc aag cgc                                    363

Phe Asn Arg Thr His Arg Ser Lys Arg                                       

        115                 120                                           





<210>  10

<211>  121

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Synthetic Construct



<400>  10



Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Val 

1               5                   10                  15      





Gln Ala Glu Pro Tyr Thr Asp Ser Asn Val Pro Glu Gly Asp Ser Val 

            20                  25                  30          





Pro Glu Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu 

        35                  40                  45              





Arg Arg Ala Arg Ser Ala Pro Thr Ala Pro Ile Ala Ala Arg Val Thr 

    50                  55                  60                  





Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg 

65                  70                  75                  80  





Arg Leu His Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Pro Thr 

                85                  90                  95      





Ser Ser Asp Thr Leu Asp Leu Asp Phe Gln Ala His Gly Thr Ile Pro 

            100                 105                 110         





Phe Asn Arg Thr His Arg Ser Lys Arg 

        115                 120     





