                         SEQUENCE LISTING

<110>  ProBioGen AG
 
<120>  ENHANCEMENT OF PROTEIN PRODUCTION YIELD MEDIATED BY A FAST 
       SHUTTLING CDC42 GTPASE

<130>  513-22 PCT

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  191
<212>  PRT
<213>  Mus musculus

<400>  1

Met Gln Thr Ile Lys Cys Val Val Val Gly Asp Gly Ala Val Gly Lys 
1               5                   10                  15      


Thr Cys Leu Leu Ile Ser Tyr Thr Thr Asn Lys Leu Pro Ser Glu Tyr 
            20                  25                  30          


Val Pro Thr Val Phe Asp Asn Tyr Ala Val Thr Val Met Ile Gly Gly 
        35                  40                  45              


Glu Pro Tyr Thr Leu Gly Leu Phe Asp Thr Ala Gly Gln Glu Asp Tyr 
    50                  55                  60                  


Asp Arg Leu Arg Pro Leu Ser Tyr Pro Gln Thr Asp Val Phe Leu Val 
65                  70                  75                  80  


Cys Phe Ser Val Val Ser Pro Ser Ser Phe Glu Asn Val Lys Glu Lys 
                85                  90                  95      


Trp Val Pro Glu Ile Thr His His Cys Pro Lys Thr Pro Phe Leu Leu 
            100                 105                 110         


Val Gly Thr Gln Ile Asp Leu Arg Asp Asp Pro Ser Thr Ile Glu Lys 
        115                 120                 125             


Leu Ala Lys Asn Lys Gln Lys Pro Ile Thr Pro Glu Thr Ala Glu Lys 
    130                 135                 140                 


Leu Ala Arg Asp Leu Lys Ala Val Lys Tyr Val Glu Cys Ser Ala Leu 
145                 150                 155                 160 


Thr Gln Lys Gly Leu Lys Asn Val Phe Asp Glu Ala Ile Leu Ala Ala 
                165                 170                 175     


Leu Glu Pro Pro Glu Pro Lys Lys Ser Arg Arg Cys Val Leu Leu 
            180                 185                 190     


<210>  2
<211>  579
<212>  DNA
<213>  artificial sequence

<220>
<223>  codon optimized nucleic acid sequence of the CDC42 variant F28L

<400>  2
atgcagacca ttaagtgcgt ggtggtcgga gatggcgccg tgggcaagac ctgcctgctg       60

atctcctaca ccaccaacaa gctgccttcc gagtacgtgc ccaccgtgtt cgacaactac      120

gccgtgaccg tgatgatcgg cggcgagcct tacaccctgg gcctgttcga caccgctggc      180

caggaagatt acgaccggct gcggcctctg tcttaccctc agaccgacgt gttcctggtc      240

tgcttctccg tggtgtcccc tagctccttc gagaacgtga aagaaaaatg ggtgcccgag      300

atcacccacc actgccctaa gacccctttc ctgctggtcg gcacccagat cgacctgcgg      360

gacgaccctt ccaccatcga gaagctggcc aagaacaagc agaagcctat cacccctgag      420

acagccgaaa agctggcccg ggacctgaag gccgtgaaat acgtggagtg ctccgccctg      480

acccagaaag gcctgaagaa cgtgttcgac gaggccatcc tggccgctct ggaacctcct      540

gagcctaaga aatcccggcg ctgcgtgctg ctgtgatga                             579


<210>  3
<211>  176
<212>  PRT
<213>  Adenovirus 5

<400>  3

Met Glu Ala Trp Glu Cys Leu Glu Asp Phe Ser Ala Val Arg Asn Leu 
1               5                   10                  15      


Leu Glu Gln Ser Ser Asn Ser Thr Ser Trp Phe Trp Arg Phe Leu Trp 
            20                  25                  30          


Gly Ser Ser Gln Ala Lys Leu Val Cys Arg Ile Lys Glu Asp Tyr Lys 
        35                  40                  45              


Trp Glu Phe Glu Glu Leu Leu Lys Ser Cys Gly Glu Leu Phe Asp Ser 
    50                  55                  60                  


Leu Asn Leu Gly His Gln Ala Leu Phe Gln Glu Lys Val Ile Lys Thr 
65                  70                  75                  80  


Leu Asp Phe Ser Thr Pro Gly Arg Ala Ala Ala Ala Val Ala Phe Leu 
                85                  90                  95      


Ser Phe Ile Lys Asp Lys Trp Ser Glu Glu Thr His Leu Ser Gly Gly 
            100                 105                 110         


Tyr Leu Leu Asp Phe Leu Ala Met His Leu Trp Arg Ala Val Val Arg 
        115                 120                 125             


His Lys Asn Arg Leu Leu Leu Leu Ser Ser Val Arg Pro Ala Ile Ile 
    130                 135                 140                 


Pro Thr Glu Glu Gln Gln Gln Gln Gln Glu Glu Ala Arg Arg Arg Arg 
145                 150                 155                 160 


Gln Glu Gln Ser Pro Trp Asn Pro Arg Ala Gly Leu Asp Pro Arg Glu 
                165                 170                 175     


<210>  4
<211>  496
<212>  PRT
<213>  Adenovirus 5

<400>  4

Met Glu Arg Arg Asn Pro Ser Glu Arg Gly Val Pro Ala Gly Phe Ser 
1               5                   10                  15      


Gly His Ala Ser Val Glu Ser Gly Cys Glu Thr Gln Glu Ser Pro Ala 
            20                  25                  30          


Thr Val Val Phe Arg Pro Pro Gly Asp Asn Thr Asp Gly Gly Ala Ala 
        35                  40                  45              


Ala Ala Ala Gly Gly Ser Gln Ala Ala Ala Ala Gly Ala Glu Pro Met 
    50                  55                  60                  


Glu Pro Glu Ser Arg Pro Gly Pro Ser Gly Met Asn Val Val Gln Val 
65                  70                  75                  80  


Ala Glu Leu Tyr Pro Glu Leu Arg Arg Ile Leu Thr Ile Thr Glu Asp 
                85                  90                  95      


Gly Gln Gly Leu Lys Gly Val Lys Arg Glu Arg Gly Ala Cys Glu Ala 
            100                 105                 110         


Thr Glu Glu Ala Arg Asn Leu Ala Phe Ser Leu Met Thr Arg His Arg 
        115                 120                 125             


Pro Glu Cys Ile Thr Phe Gln Gln Ile Lys Asp Asn Cys Ala Asn Glu 
    130                 135                 140                 


Leu Asp Leu Leu Ala Gln Lys Tyr Ser Ile Glu Gln Leu Thr Thr Tyr 
145                 150                 155                 160 


Trp Leu Gln Pro Gly Asp Asp Phe Glu Glu Ala Ile Arg Val Tyr Ala 
                165                 170                 175     


Lys Val Ala Leu Arg Pro Asp Cys Lys Tyr Lys Ile Ser Lys Leu Val 
            180                 185                 190         


Asn Ile Arg Asn Cys Cys Tyr Ile Ser Gly Asn Gly Ala Glu Val Glu 
        195                 200                 205             


Ile Asp Thr Glu Asp Arg Val Ala Phe Arg Cys Ser Met Ile Asn Met 
    210                 215                 220                 


Trp Pro Gly Val Leu Gly Met Asp Gly Val Val Ile Met Asn Val Arg 
225                 230                 235                 240 


Phe Thr Gly Pro Asn Phe Ser Gly Thr Val Phe Leu Ala Asn Thr Asn 
                245                 250                 255     


Leu Ile Leu His Gly Val Ser Phe Tyr Gly Phe Asn Asn Thr Cys Val 
            260                 265                 270         


Glu Ala Trp Thr Asp Val Arg Val Arg Gly Cys Ala Phe Tyr Cys Cys 
        275                 280                 285             


Trp Lys Gly Val Val Cys Arg Pro Lys Ser Arg Ala Ser Ile Lys Lys 
    290                 295                 300                 


Cys Leu Phe Glu Arg Cys Thr Leu Gly Ile Leu Ser Glu Gly Asn Ser 
305                 310                 315                 320 


Arg Val Arg His Asn Val Ala Ser Asp Cys Gly Cys Phe Met Leu Val 
                325                 330                 335     


Lys Ser Val Ala Val Ile Lys His Asn Met Val Cys Gly Asn Cys Glu 
            340                 345                 350         


Asp Arg Ala Ser Gln Met Leu Thr Cys Ser Asp Gly Asn Cys His Leu 
        355                 360                 365             


Leu Lys Thr Ile His Val Ala Ser His Ser Arg Lys Ala Trp Pro Val 
    370                 375                 380                 


Phe Glu His Asn Ile Leu Thr Arg Cys Ser Leu His Leu Gly Asn Arg 
385                 390                 395                 400 


Arg Gly Val Phe Leu Pro Tyr Gln Cys Asn Leu Ser His Thr Lys Ile 
                405                 410                 415     


Leu Leu Glu Pro Glu Ser Met Ser Lys Val Asn Leu Asn Gly Val Phe 
            420                 425                 430         


Asp Met Thr Met Lys Ile Trp Lys Val Leu Arg Tyr Asp Glu Thr Arg 
        435                 440                 445             


Thr Arg Cys Arg Pro Cys Glu Cys Gly Gly Lys His Ile Arg Asn Gln 
    450                 455                 460                 


Pro Val Met Leu Asp Val Thr Glu Glu Leu Arg Pro Asp His Leu Val 
465                 470                 475                 480 


Leu Ala Cys Thr Arg Ala Glu Phe Gly Ser Ser Asp Glu Asp Thr Asp 
                485                 490                 495     


<210>  5
<211>  191
<212>  PRT
<213>  Mus musculus

<400>  5

Met Gln Thr Ile Lys Cys Val Val Val Gly Asp Gly Ala Val Gly Lys 
1               5                   10                  15      


Thr Cys Leu Leu Ile Ser Tyr Thr Thr Asn Lys Phe Pro Ser Glu Tyr 
            20                  25                  30          


Val Pro Thr Val Phe Asp Asn Tyr Ala Val Thr Val Met Ile Gly Gly 
        35                  40                  45              


Glu Pro Tyr Thr Leu Gly Leu Phe Asp Thr Ala Gly Gln Glu Asp Tyr 
    50                  55                  60                  


Asp Arg Leu Arg Pro Leu Ser Tyr Pro Gln Thr Asp Val Phe Leu Val 
65                  70                  75                  80  


Cys Phe Ser Val Val Ser Pro Ser Ser Phe Glu Asn Val Lys Glu Lys 
                85                  90                  95      


Trp Val Pro Glu Ile Thr His His Cys Pro Lys Thr Pro Phe Leu Leu 
            100                 105                 110         


Val Gly Thr Gln Ile Asn Leu Arg Asp Asp Pro Ser Thr Ile Glu Lys 
        115                 120                 125             


Leu Ala Lys Asn Lys Gln Lys Pro Ile Thr Pro Glu Thr Ala Glu Lys 
    130                 135                 140                 


Leu Ala Arg Asp Leu Lys Ala Val Lys Tyr Val Glu Cys Ser Ala Leu 
145                 150                 155                 160 


Thr Gln Lys Gly Leu Lys Asn Val Phe Asp Glu Ala Ile Leu Ala Ala 
                165                 170                 175     


Leu Glu Pro Pro Glu Pro Lys Lys Ser Arg Arg Cys Val Leu Leu 
            180                 185                 190     


<210>  6
<211>  191
<212>  PRT
<213>  Mus musculus

<400>  6

Met Gln Thr Ile Lys Cys Val Val Val Gly Asp Gly Ala Val Gly Lys 
1               5                   10                  15      


Thr Cys Leu Leu Ile Ser Tyr Thr Thr Asn Lys Leu Pro Ser Glu Tyr 
            20                  25                  30          


Val Pro Thr Val Phe Asp Asn Tyr Ala Val Thr Val Met Ile Gly Gly 
        35                  40                  45              


Glu Pro Tyr Thr Leu Gly Leu Phe Asp Thr Ala Gly Gln Glu Asp Tyr 
    50                  55                  60                  


Asp Arg Leu Arg Pro Leu Ser Tyr Pro Gln Thr Asp Val Phe Leu Val 
65                  70                  75                  80  


Cys Phe Ser Val Val Ser Pro Ser Ser Phe Glu Asn Val Lys Glu Lys 
                85                  90                  95      


Trp Val Pro Glu Ile Thr His His Cys Pro Lys Thr Pro Phe Leu Leu 
            100                 105                 110         


Val Gly Thr Gln Ile Asp Leu Arg Asn Asp Pro Ser Thr Ile Glu Lys 
        115                 120                 125             


Leu Ala Lys Asn Lys Gln Lys Pro Ile Thr Pro Glu Thr Ala Glu Lys 
    130                 135                 140                 


Leu Ala Arg Asp Leu Lys Ala Val Lys Tyr Val Glu Cys Ser Ala Leu 
145                 150                 155                 160 


Thr Gln Lys Gly Leu Lys Asn Val Phe Asp Glu Ala Ile Leu Ala Ala 
                165                 170                 175     


Leu Glu Pro Pro Glu Pro Lys Lys Ser Arg Arg Cys Val Leu Leu 
            180                 185                 190     


<210>  7
<211>  1798
<212>  DNA
<213>  Adenovirus 5

<400>  7
atggaggctt gggagtgttt ggaagatttt tctgctgtgc gtaacttgct ggaacagagc       60

tctaacagta cctcttggtt ttggaggttt ctgtggggct catcccaggc aaagttagtc      120

tgcagaatta aggaggatta caagtgggaa tttgaagagc ttttgaaatc ctgtggtgag      180

ctgtttgatt ctttgaatct gggtcaccag gcgcttttcc aagagaaggt catcaagact      240

ttggattttt ccacaccggg gcgcgctgcg gctgctgttg cttttttgag ttttataaag      300

gataaatgga gcgaagaaac ccatctgagc ggggggtacc tgctggattt tctggccatg      360

catctgtgga gagcggttgt gagacacaag aatcgcctgc tactgttgtc ttccgtccgc      420

ccggcgataa taccgacgga ggagcagcag cagcagcagg aggaagccag gcggcggcgg      480

caggagcaga gcccatggaa cccgagagcc ggcctggacc ctcgggaatg aatgttgtac      540

aggtggctga actgtatcca gaactgagac gcattttgac aattacagag gatgggcagg      600

ggctaaaggg ggtaaagagg gagcgggggg cttgtgaggc tacagaggag gctaggaatc      660

tagcttttag cttaatgacc agacaccgtc ctgagtgtat tacttttcaa cagatcaagg      720

ataattgcgc taatgagctt gatctgctgg cgcagaagta ttccatagag cagctgacca      780

cttactggct gcagccaggg gatgattttg aggaggctat tagggtatat gcaaaggtgg      840

cacttaggcc agattgcaag tacaagatca gcaaacttgt aaatatcagg aattgttgct      900

acatttctgg gaacggggcc gaggtggaga tagatacgga ggatagggtg gcctttagat      960

gtagcatgat aaatatgtgg ccgggggtgc ttggcatgga cggggtggtt attatgaatg     1020

taaggtttac tggccccaat tttagcggta cggttttcct ggccaatacc aaccttatcc     1080

tacacggtgt aagcttctat gggtttaaca atacctgtgt ggaagcctgg accgatgtaa     1140

gggttcgggg ctgtgccttt tactgctgct ggaagggggt ggtgtgtcgc cccaaaagca     1200

gggcttcaat taagaaatgc ctctttgaaa ggtgtacctt gggtatcctg tctgagggta     1260

actccagggt gcgccacaat gtggcctccg actgtggttg cttcatgcta gtgaaaagcg     1320

tggctgtgat taagcataac atggtatgtg gcaactgcga ggacagggcc tctcagatgc     1380

tgacctgctc ggacggcaac tgtcacctgc tgaagaccat tcacgtagcc agccactctc     1440

gcaaggcctg gccagtgttt gagcataaca tactgacccg ctgttccttg catttgggta     1500

acaggagggg ggtgttccta ccttaccaat gcaatttgag tcacactaag atattgcttg     1560

agcccgagag catgtccaag gtgaacctga acggggtgtt tgacatgacc atgaagatct     1620

ggaaggtgct gaggtacgat gagacccgca ccaggtgcag accctgcgag tgtggcggta     1680

aacatattag gaaccagcct gtgatgctgg atgtgaccga ggagctgagg cccgatcact     1740

tggtgctggc ctgcacccgc gctgagtttg gctctagcga tgaagataca gattgagg       1798


<210>  8
<211>  191
<212>  PRT
<213>  Mus musculus

<400>  8

Met Gln Thr Ile Lys Cys Val Val Val Gly Asp Gly Ala Val Gly Lys 
1               5                   10                  15      


Thr Cys Leu Leu Ile Ser Tyr Thr Thr Asn Lys Phe Pro Ser Glu Tyr 
            20                  25                  30          


Val Pro Thr Val Phe Asp Asn Tyr Ala Val Thr Val Met Ile Gly Gly 
        35                  40                  45              


Glu Pro Tyr Thr Leu Gly Leu Phe Asp Thr Ala Gly Gln Glu Asp Tyr 
    50                  55                  60                  


Asp Arg Leu Arg Pro Leu Ser Tyr Pro Gln Thr Asp Val Phe Leu Val 
65                  70                  75                  80  


Cys Phe Ser Val Val Ser Pro Ser Ser Phe Glu Asn Val Lys Glu Lys 
                85                  90                  95      


Trp Val Pro Glu Ile Thr His His Cys Pro Lys Thr Pro Phe Leu Leu 
            100                 105                 110         


Val Gly Thr Gln Ile Asp Leu Arg Asp Asp Pro Ser Thr Ile Glu Lys 
        115                 120                 125             


Leu Ala Lys Asn Lys Gln Lys Pro Ile Thr Pro Glu Thr Ala Glu Lys 
    130                 135                 140                 


Leu Ala Arg Asp Leu Lys Ala Val Lys Tyr Val Glu Cys Ser Ala Leu 
145                 150                 155                 160 


Thr Gln Lys Gly Leu Lys Asn Val Phe Asp Glu Ala Ile Leu Ala Ala 
                165                 170                 175     


Leu Glu Pro Pro Glu Pro Lys Lys Ser Arg Arg Cys Val Leu Leu 
            180                 185                 190     


