
                                SEQUENCE LISTING

<110> Merck Sharp & Dohme Corp.
      Coller, Beth-Ann Griswold
      Pai, Vidya B.
      Parks, D. Elliot
      Yelmene, Michele
      Bett, Andrew J.
      Martyak, Timothy

<120> RECOMBINANT SUBUNIT DENGUE VIRUS VACCINE
  

<130> MRL-IFD-00058

<150> 61/408,310          
<151> 2010-10-29  

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1683
<212> DNA
<213> Dengue virus type 1

<400> 1
ttccatctga ccacacgagg gggagagccg cacatgatag ttagcaagca ggaaagagga 60
aagtcacttt tgtttaagac ctcagcaggt gtcaacatgt gcacccttat agcgatggat 120
ttgggagagt tatgtgagga cacaatgact tacaaatgcc ctcgaattac tgaggcggaa 180
ccagatgacg ttgattgttg gtgcaatgct acagacacat gggtgaccta tggaacatgt 240
tcccaaactg gcgagcaccg acgggacaaa cgttccgtcg cactggcccc acacgtggga 300
cttggtttgg aaacaagaac cgaaacgtgg atgtcctctg aaggcgcttg gaaacagata 360
caaagagtgg agacttgggc cctgagacac ccaggattca cggtgatagc cctttttcta 420
gcacatgcca taggaacatc catcacccaa aaagggatta ttttcatttt gttaatgcta 480
gtaacaccat ccatggccat gcgatgcgtg ggaataggca gcagggactt cgtggaagga 540
ctgtcaggag caacttgggt agatgtggta ctggaacatg gaagttgcgt caccaccatg 600
gcaaaagaca aaccaacatt ggacattgaa ctcttgaaga cggaagtcac aaaccctgcc 660
gtcctgcgca aactgtgcat tgaagctaaa atatcaaaca ccaccaccga ttcaagatgt 720
ccaacacaag gagaagccac actggtggaa gaacaagacg cgaactttgt gtgtcgacga 780
acgtttgtgg acagaggctg gggcaatggc tgtgggctct tcggaaaagg tagcctaata 840
acgtgtgcta agttcaagtg tgtgacaaaa ctggaaggaa agatagttca atacgaaaac 900
ttgaaatatt cagtaatagt caccgtccac actggagacc agcaccaggt gggaaatgaa 960
agcacagaac atgggacaac tgcaactata acacctcaag ctcctacgtc ggaaatacag 1020
ctgaccgact acggagctct tacattggat tgctcaccta gaacaggact ggactttaat 1080
gaaatggtgt tgttgacaat gaaagaaaaa tcatggctag tccacaaaca atggtttcta 1140
gacctaccac tgccttggac ctcgggagct tcaacatcac aagagacttg gaacagacaa 1200
gatttgctgg taacatttaa gacagcccat gcaaagaagc aggaagtagt cgtactagga 1260
tcacaagaag gagcaatgca cactgcgttg accggagcga cagaaatcca aacgtctgga 1320
acgacaacaa tttttgcagg acacctgaaa tgtagactaa aaatggacaa actgactcta 1380
aaagggatgt catatgttat gtgcacaggc tcattcaagc tagagaaaga agtggctgag 1440
acccagcatg gaaccgttct agtgcagatt aaatacgaag gaacagatgc accatgcaag 1500
atcccttttt cgacccaaga tgaaagagga gtaacccaga acgggagatt aataacagcc 1560
aaccctatag ttactgacaa agaaaaacca gtcaacattg aggcagaacc gccttttggt 1620
gagagttaca tcgtgatagg agcaggtgaa aaagctttga aactaagctg gttcaagaag 1680
gga                                                               1683

<210> 2
<211> 1683
<212> DNA
<213> Dengue virus type 2

<400> 2
tttcatctga ccacacgcaa cggagaacca cacatgatcg tcagtagaca agaaaaaggg 60
aaaagccttc tgtttaagac aaaggacggc acgaacatgt gtaccctcat ggccatggac 120
cttggtgagt tgtgtgaaga cacaatcacg tataaatgtc cctttctcaa gcagaacgaa 180
ccagaagaca tagattgttg gtgcaactcc acgtccacat gggtaactta tgggacatgt 240
accaccacag gagagcacag aagagaaaaa agatcagtgg cgcttgttcc acacgtggga 300
atgggattgg agacacgaac tgaaacatgg atgtcatcag aaggggcctg gaaacatgcc 360
cagagaattg aaacttggat tctgagacat ccaggcttta ccataatggc cgcaatcctg 420
gcatacacca taggaacgac gcatttccaa agagtcctga tattcatcct actgacagcc 480
atcgctcctt caatgacaat gcgctgcata ggaatatcaa atagggactt tgtggaagga 540
gtgtcaggag ggagttgggt tgacatagtt ttagaacatg gaagttgtgt gacgacgatg 600
gcaaaaaata aaccaacact ggactttgaa ctgataaaaa cagaagccaa acaacccgcc 660
accttaagga agtactgtat agaggctaaa ctgaccaaca cgacaacaga ctcgcgctgc 720
ccaacacaag gggaacccac cctgaatgaa gagcaggaca aaaggtttgt ctgcaaacat 780
tccatggtag acagaggatg gggaaatgga tgtggattat ttggaaaagg aggcatcgtg 840
acctgtgcca tgttcacatg caaaaagaac atggagggaa aaattgtgca gccagaaaac 900
ctggaataca ctgtcgttat aacacctcat tcaggggaag aacatgcagt cggaaatgac 960
acaggaaaac atggtaaaga agtcaagata acaccacaga gctccatcac agaggcggaa 1020
ctgacaggct atggcactgt tacgatggag tgctctccaa gaacgggcct cgacttcaat 1080
gagatggtgt tgctgcaaat gaaagacaaa gcttggctgg tgcacagaca atggttccta 1140
gacctaccgt tgccatggct gcccggagca gacacacaag gatcaaattg gatacagaaa 1200
gagacactgg tcaccttcaa aaatccccat gcgaaaaaac aggatgttgt tgtcttagga 1260
tcccaagagg gggccatgca tacagcactc acaggggcta cggaaatcca gatgtcatca 1320
ggaaacctgc tgttcacagg acatcttaag tgcaggctga gaatggacaa attacaactt 1380
aaagggatgt catactccat gtgcacagga aagtttaaag ttgtgaagga aatagcagaa 1440
acacaacatg gaacaatagt cattagagta caatatgaag gagacggctc tccatgcaag 1500
atcccttttg agataatgga tctggaaaaa agacatgttt tgggccgcct gatcacagtc 1560
aatccaattg taacagaaaa ggacagccca gtcaacatag aagcagaacc tccattcgga 1620
gacagctaca tcatcatagg agtggaacca ggacaattga agctggactg gttcaagaaa 1680
gga                                                               1683

<210> 3
<211> 1677
<212> DNA
<213> Dengue virus type 3

<400> 3
ttccacttga cttcacgcga tggagagccg cgcatgattg tggggaagaa tgaaagaggg 60
aaatccctac tttttaagac agcttctgga atcaacatgt gcacactcat agccatggac 120
ttgggagaga tgtgtgatga cacggtcact tacaaatgcc cccacattgc cgaagtggaa 180
cctgaagaca ttgactgctg gtgcaacctt acatcgacat gggtgactta tggaacgtgc 240
aatcaagctg gggagcacag acgcgacaag agatcagtgg cgttagctcc ccatgtcggc 300
atgggactgg acacacgcac ccaaacctgg atgtcggctg aaggagcctg gagacaagtc 360
gagaaggtag agacatgggc ccttaggcac ccagggttca ccatactagc tctatttctt 420
gcccattaca taggcacttc cttgacccag aaagtggtta tttttatact actaatactg 480
gtcactccat ccatggcaat gagatgcgtg ggagtaggaa acagagattt tgtggaaggt 540
ctatcgggag ctacgtgggt tgacgtggtg ctcgagcacg gtgggtgtgt gaccaccatg 600
gctaagaaca agcccacgct ggacatagag cttcagaaga ccgaggccac ccaactggcg 660
accctaagga agttatgcat tgagggaaaa attaccaaca taacaactga ctcaaggtgt 720
cctacccagg gggaagcgat tttacctgag gagcaggacc agaactacgt atgtaagcat 780
acatacgtgg atagaggctg gggaaacggt tgtggtttgt ttggaaaagg aagcttggtg 840
acatgcgcga aatttcaatg cttagaatca atagagggaa aagtggtgca acatgagaac 900
ctcaaataca ctgtcatcat tacagtgcac acaggagacc aacaccaggt gggaaatgaa 960
acgcagggag tcacggctga gataacaccc caggcatcaa ccgttgaagc tatcttgcct 1020
gaatatggaa cccttgggct agaatgctca ccacggacag gtttggattt caatgaaatg 1080
atcttattga caatgaagaa caaagcatgg atggtacata gacaatggtt ctttgaccta 1140
cccctaccat ggacatcagg agctacaaca gagacaccaa cttggaacag gaaagagctt 1200
cttgtgacat tcaaaaatgc acatgcaaaa aagcaagaag tagttgtcct tggatcgcaa 1260
gagggagcaa tgcacacagc gctgacagga gctacagaga tccaaaactc aggaggcaca 1320
agcatttttg cggggcactt gaaatgtaga cttaagatgg acaaattgga actcaagggg 1380
atgagctatg caatgtgctt gaacaccttt gtgttgaaga aagaagtctc cgagacgcag 1440
catgggacaa tactcattaa ggttgagtac aaaggggaag atgcaccttg caagattcct 1500
ttctccacgg aggatggaca agggaaagct cacaatggta gactgatcac agccaaccca 1560
gtggtgacca agaaggagga gcctgtcaac attgaggctg aacctccttt tggggaaagt 1620
aacatagtga ttggaattgg agacaaagcc ttgaaaatta actggtacaa gaaggga    1677

<210> 4
<211> 1683
<212> DNA
<213> Dengue virus type 4

<400> 4
tttcacttgt caacaagaga tggcgaaccc cttatgatag tggcaaaaca cgaaaggggg 60
agacctctct tgtttaagac aacagaggga atcaacaaat gcactcttat tgccatggac 120
ctgggtgaaa tgtgtgagga caccgtcacg tatgaatgcc ctctactggt caataccgaa 180
cctgaggaca ttgattgctg gtgcaatctc acgtctgcct gggtcatgta tgggacatgc 240
actcagagtg gggaacggag acgggagaag cgctcagtag ccctaacacc acattcagga 300
atgggattgg agacaagggc tgagacatgg atgtcatcgg aaggggcttg gaaacatgct 360
cagagggtag agagttggat actcagaaac ccaggattcg ctctcttggc aggatttatg 420
gcctatatga ttgggcaaac aggaatccag cgaacagtct tctttgttct aatgatgctg 480
gtcgccccat cctacggaat gcgatgcgtg ggagtgggga acagagactt tgtggaagga 540
gtctcaggtg gagcatgggt cgatttggtg ctagaacatg gaggatgtgt cacaaccatg 600
gcccagggaa aaccaacctt ggattttgaa ctgatcaaga caacagccaa ggaagtggct 660
ctgttaagaa cctattgcat tgaagcctcg atatcaaaca taaccacggc aacaagatgt 720
ccaacgcaag gagaacctta tctcaaagag gaacaagatc aacagtacat ttgccggaga 780
gatgtggtag acagagggtg gggcaatggc tgtggcttgt ttgggaaagg aggagttgtg 840
acatgtgcga agttttcatg ctcggggaag ataacaggca atttggtcca aattgagaac 900
cttgaataca cagtagttgt aacagtccac aatggagaca cccatgcagt aggaaatgac 960
acatccaacc atggagtgac agccacgata acccccaggt caccatcggt agaagttaaa 1020
ttaccggatt atggagaatt aacactcgat tgtgaaccca ggtccggaat tgattttaat 1080
gagatgattc tgatgaaaat gaaaaagaaa acgtggcttg tgcacaagca atggtttttg 1140
gatctacctc taccatgggc agcaggagca gacacatcag aagttcattg gaattacaaa 1200
gagagaatgg tgacattcaa ggttcctcat gccaagagac aggatgtgac agtgctagga 1260
tctcaggaag gagccatgca ttctgccctc accggagcta cagaagtgga ttccggtgat 1320
ggaaaccaca tgtatgcagg acatctgaaa tgcaaagttc gcatggagaa attgagaatt 1380
aagggaatgt catacacgat gtgctcagga aagttctcaa ttgacaaaga gatggcagaa 1440
acacagcatg ggacaacagt ggtaaaagtc aagtatgagg gtgctggagc tccatgtaaa 1500
gttcccatag agataagaga tgtgaacaag gaaaaagtgg tagggcgcat catctcatct 1560
accccttttg ctgagtatac caacagtgta accaacatag aattagaacc cccctttggg 1620
gacagctaca tagtaatagg tgttggagac agtgcattaa cactccattg gttcaggaaa 1680
ggg                                                               1683

<210> 5
<211> 1851
<212> DNA
<213> Artificial Sequence

<220> 
<223> DEN4 prM-80Ezip

<400> 5
tttcacttgt caacaagaga tggcgaaccc cttatgatag tggcaaaaca cgaaaggggg 60
agacctctct tgtttaagac aacagaggga atcaacaaat gcactcttat tgccatggac 120
ctgggtgaaa tgtgtgagga caccgtcacg tatgaatgcc ctctactggt caataccgaa 180
cctgaggaca ttgattgctg gtgcaatctc acgtctgcct gggtcatgta tgggacatgc 240
actcagagtg gggaacggag acgggagaag cgctcagtag ccctaacacc acattcagga 300
atgggattgg agacaagggc tgagacatgg atgtcatcgg aaggggcttg gaaacatgct 360
cagagggtag agagttggat actcagaaac ccaggattcg ctctcttggc aggatttatg 420
gcctatatga ttgggcaaac aggaatccag cgaacagtct tctttgttct aatgatgctg 480
gtcgccccat cctacggaat gcgatgcgtg ggagtgggga acagagactt tgtggaagga 540
gtctcaggtg gagcatgggt cgatttggtg ctagaacatg gaggatgtgt cacaaccatg 600
gcccagggaa aaccaacctt ggattttgaa ctgatcaaga caacagccaa ggaagtggct 660
ctgttaagaa cctattgcat tgaagcctcg atatcaaaca taaccacggc aacaagatgt 720
ccaacgcaag gagaacctta tctcaaagag gaacaagatc aacagtacat ttgccggaga 780
gatgtggtag acagagggtg gggcaatggc tgtggcttgt ttgggaaagg aggagttgtg 840
acatgtgcga agttttcatg ctcggggaag ataacaggca atttggtcca aattgagaac 900
cttgaataca cagtagttgt aacagtccac aatggagaca cccatgcagt aggaaatgac 960
acatccaacc atggagtgac agccacgata acccccaggt caccatcggt agaagttaaa 1020
ttaccggatt atggagaatt aacactcgat tgtgaaccca ggtccggaat tgattttaat 1080
gagatgattc tgatgaaaat gaaaaagaaa acgtggcttg tgcacaagca atggtttttg 1140
gatctacctc taccatgggc agcaggagca gacacatcag aagttcattg gaattacaaa 1200
gagagaatgg tgacattcaa ggttcctcat gccaagagac aggatgtgac agtgctagga 1260
tctcaggaag gagccatgca ttctgccctc accggagcta cagaagtgga ttccggtgat 1320
ggaaaccaca tgtatgcagg acatctgaaa tgcaaagttc gcatggagaa attgagaatt 1380
aagggaatgt catacacgat gtgctcagga aagttctcaa ttgacaaaga gatggcagaa 1440
acacagcatg ggacaacagt ggtaaaagtc aagtatgagg gtgctggagc tccatgtaaa 1500
gttcccatag agataagaga tgtgaacaag gaaaaagtgg tagggcgcat catctcatct 1560
accccttttg ctgagtatac caacagtgta accaacatag aattagaacc cccctttggg 1620
gacagctaca tagtaatagg tgttggagac agtgcattaa cactccattg gttcaggaaa 1680
gggggtggtg gttctggtgg tggtggtacc ggcggtggct ccggcggtgg ctccccccgc 1740
atgaagcagc tggaggacaa ggtggaggag ctgctgtcca agaactacca cctggagaac 1800
gaggtggccc gcctgaagaa gctggtgggc gagcgcggcg gttgcggcgg t          1851

<210> 6
<211> 395
<212> PRT
<213> Dengue virus type 1

<400> 6
Met Arg Cys Val Gly Ile Gly Ser Arg Asp Phe Val Glu Gly Leu Ser
 1               5                  10                  15      
Gly Ala Thr Trp Val Asp Val Val Leu Glu His Gly Ser Cys Val Thr
            20                  25                  30          
Thr Met Ala Lys Asp Lys Pro Thr Leu Asp Ile Glu Leu Leu Lys Thr
        35                  40                  45              
Glu Val Thr Asn Pro Ala Val Leu Arg Lys Leu Cys Ile Glu Ala Lys
    50                  55                  60                  
Ile Ser Asn Thr Thr Thr Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65                  70                  75                  80  
Thr Leu Val Glu Glu Gln Asp Ala Asn Phe Val Cys Arg Arg Thr Phe
                85                  90                  95      
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
            100                 105                 110         
Leu Ile Thr Cys Ala Lys Phe Lys Cys Val Thr Lys Leu Glu Gly Lys
        115                 120                 125             
Ile Val Gln Tyr Glu Asn Leu Lys Tyr Ser Val Ile Val Thr Val His
    130                 135                 140                 
Thr Gly Asp Gln His Gln Val Gly Asn Glu Ser Thr Glu His Gly Thr
145                 150                 155                 160 
Thr Ala Thr Ile Thr Pro Gln Ala Pro Thr Ser Glu Ile Gln Leu Thr
                165                 170                 175     
Asp Tyr Gly Ala Leu Thr Leu Asp Cys Ser Pro Arg Thr Gly Leu Asp
            180                 185                 190         
Phe Asn Glu Met Val Leu Leu Thr Met Lys Glu Lys Ser Trp Leu Val
        195                 200                 205             
His Lys Gln Trp Phe Leu Asp Leu Pro Leu Pro Trp Thr Ser Gly Ala
    210                 215                 220                 
Ser Thr Ser Gln Glu Thr Trp Asn Arg Gln Asp Leu Leu Val Thr Phe
225                 230                 235                 240 
Lys Thr Ala His Ala Lys Lys Gln Glu Val Val Val Leu Gly Ser Gln
                245                 250                 255     
Glu Gly Ala Met His Thr Ala Leu Thr Gly Ala Thr Glu Ile Gln Thr
            260                 265                 270         
Ser Gly Thr Thr Thr Ile Phe Ala Gly His Leu Lys Cys Arg Leu Lys
        275                 280                 285             
Met Asp Lys Leu Thr Leu Lys Gly Met Ser Tyr Val Met Cys Thr Gly
    290                 295                 300                 
Ser Phe Lys Leu Glu Lys Glu Val Ala Glu Thr Gln His Gly Thr Val
305                 310                 315                 320 
Leu Val Gln Ile Lys Tyr Glu Gly Thr Asp Ala Pro Cys Lys Ile Pro
                325                 330                 335     
Phe Ser Thr Gln Asp Glu Arg Gly Val Thr Gln Asn Gly Arg Leu Ile
            340                 345                 350         
Thr Ala Asn Pro Ile Val Thr Asp Lys Glu Lys Pro Val Asn Ile Glu
        355                 360                 365             
Ala Glu Pro Pro Phe Gly Glu Ser Tyr Ile Val Ile Gly Ala Gly Glu
    370                 375                 380                 
Lys Ala Leu Lys Leu Ser Trp Phe Lys Lys Gly
385                 390                 395 


<210> 7
<211> 395
<212> PRT
<213> Dengue virus type 2

<400> 7
Met Arg Cys Ile Gly Ile Ser Asn Arg Asp Phe Val Glu Gly Val Ser
 1               5                  10                  15      
Gly Gly Ser Trp Val Asp Ile Val Leu Glu His Gly Ser Cys Val Thr
            20                  25                  30          
Thr Met Ala Lys Asn Lys Pro Thr Leu Asp Phe Glu Leu Ile Lys Thr
        35                  40                  45              
Glu Ala Lys Gln Pro Ala Thr Leu Arg Lys Tyr Cys Ile Glu Ala Lys
    50                  55                  60                  
Leu Thr Asn Thr Thr Thr Asp Ser Arg Cys Pro Thr Gln Gly Glu Pro
65                  70                  75                  80  
Thr Leu Asn Glu Glu Gln Asp Lys Arg Phe Val Cys Lys His Ser Met
                85                  90                  95      
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Gly
            100                 105                 110         
Ile Val Thr Cys Ala Met Phe Thr Cys Lys Lys Asn Met Glu Gly Lys
        115                 120                 125             
Ile Val Gln Pro Glu Asn Leu Glu Tyr Thr Val Val Ile Thr Pro His
    130                 135                 140                 
Ser Gly Glu Glu His Ala Val Gly Asn Asp Thr Gly Lys His Gly Lys
145                 150                 155                 160 
Glu Val Lys Ile Thr Pro Gln Ser Ser Ile Thr Glu Ala Glu Leu Thr
                165                 170                 175     
Gly Tyr Gly Thr Val Thr Met Glu Cys Ser Pro Arg Thr Gly Leu Asp
            180                 185                 190         
Phe Asn Glu Met Val Leu Leu Gln Met Lys Asp Lys Ala Trp Leu Val
        195                 200                 205             
His Arg Gln Trp Phe Leu Asp Leu Pro Leu Pro Trp Leu Pro Gly Ala
    210                 215                 220                 
Asp Thr Gln Gly Ser Asn Trp Ile Gln Lys Glu Thr Leu Val Thr Phe
225                 230                 235                 240 
Lys Asn Pro His Ala Lys Lys Gln Asp Val Val Val Leu Gly Ser Gln
                245                 250                 255     
Glu Gly Ala Met His Thr Ala Leu Thr Gly Ala Thr Glu Ile Gln Met
            260                 265                 270         
Ser Ser Gly Asn Leu Leu Phe Thr Gly His Leu Lys Cys Arg Leu Arg
        275                 280                 285             
Met Asp Lys Leu Gln Leu Lys Gly Met Ser Tyr Ser Met Cys Thr Gly
    290                 295                 300                 
Lys Phe Lys Val Val Lys Glu Ile Ala Glu Thr Gln His Gly Thr Ile
305                 310                 315                 320 
Val Ile Arg Val Gln Tyr Glu Gly Asp Gly Ser Pro Cys Lys Ile Pro
                325                 330                 335     
Phe Glu Ile Met Asp Leu Glu Lys Arg His Val Leu Gly Arg Leu Ile
            340                 345                 350         
Thr Val Asn Pro Ile Val Thr Glu Lys Asp Ser Pro Val Asn Ile Glu
        355                 360                 365             
Ala Glu Pro Pro Phe Gly Asp Ser Tyr Ile Ile Ile Gly Val Glu Pro
    370                 375                 380                 
Gly Gln Leu Lys Leu Asp Trp Phe Lys Lys Gly
385                 390                 395 


<210> 8
<211> 393
<212> PRT
<213> Dengue virus type 3

<400> 8
Met Arg Cys Val Gly Val Gly Asn Arg Asp Phe Val Glu Gly Leu Ser
 1               5                  10                  15      
Gly Ala Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
            20                  25                  30          
Thr Met Ala Lys Asn Lys Pro Thr Leu Asp Ile Glu Leu Gln Lys Thr
        35                  40                  45              
Glu Ala Thr Gln Leu Ala Thr Leu Arg Lys Leu Cys Ile Glu Gly Lys
    50                  55                  60                  
Ile Thr Asn Ile Thr Thr Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65                  70                  75                  80  
Ile Leu Pro Glu Glu Gln Asp Gln Asn Tyr Val Cys Lys His Thr Tyr
                85                  90                  95      
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
            100                 105                 110         
Leu Val Thr Cys Ala Lys Phe Gln Cys Leu Glu Ser Ile Glu Gly Lys
        115                 120                 125             
Val Val Gln His Glu Asn Leu Lys Tyr Thr Val Ile Ile Thr Val His
    130                 135                 140                 
Thr Gly Asp Gln His Gln Val Gly Asn Glu Thr Gln Gly Val Thr Ala
145                 150                 155                 160 
Glu Ile Thr Pro Gln Ala Ser Thr Val Glu Ala Ile Leu Pro Glu Tyr
                165                 170                 175     
Gly Thr Leu Gly Leu Glu Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn
            180                 185                 190         
Glu Met Ile Leu Leu Thr Met Lys Asn Lys Ala Trp Met Val His Arg
        195                 200                 205             
Gln Trp Phe Phe Asp Leu Pro Leu Pro Trp Thr Ser Gly Ala Thr Thr
    210                 215                 220                 
Glu Thr Pro Thr Trp Asn Arg Lys Glu Leu Leu Val Thr Phe Lys Asn
225                 230                 235                 240 
Ala His Ala Lys Lys Gln Glu Val Val Val Leu Gly Ser Gln Glu Gly
                245                 250                 255     
Ala Met His Thr Ala Leu Thr Gly Ala Thr Glu Ile Gln Asn Ser Gly
            260                 265                 270         
Gly Thr Ser Ile Phe Ala Gly His Leu Lys Cys Arg Leu Lys Met Asp
        275                 280                 285             
Lys Leu Glu Leu Lys Gly Met Ser Tyr Ala Met Cys Leu Asn Thr Phe
    290                 295                 300                 
Val Leu Lys Lys Glu Val Ser Glu Thr Gln His Gly Thr Ile Leu Ile
305                 310                 315                 320 
Lys Val Glu Tyr Lys Gly Glu Asp Ala Pro Cys Lys Ile Pro Phe Ser
                325                 330                 335     
Thr Glu Asp Gly Gln Gly Lys Ala His Asn Gly Arg Leu Ile Thr Ala
            340                 345                 350         
Asn Pro Val Val Thr Lys Lys Glu Glu Pro Val Asn Ile Glu Ala Glu
        355                 360                 365             
Pro Pro Phe Gly Glu Ser Asn Ile Val Ile Gly Ile Gly Asp Lys Ala
    370                 375                 380                 
Leu Lys Ile Asn Trp Tyr Lys Lys Gly
385                 390             


<210> 9
<211> 395
<212> PRT
<213> Dengue virus type 4

<400> 9
Met Arg Cys Val Gly Val Gly Asn Arg Asp Phe Val Glu Gly Val Ser
 1               5                  10                  15      
Gly Gly Ala Trp Val Asp Leu Val Leu Glu His Gly Gly Cys Val Thr
            20                  25                  30          
Thr Met Ala Gln Gly Lys Pro Thr Leu Asp Phe Glu Leu Ile Lys Thr
        35                  40                  45              
Thr Ala Lys Glu Val Ala Leu Leu Arg Thr Tyr Cys Ile Glu Ala Ser
    50                  55                  60                  
Ile Ser Asn Ile Thr Thr Ala Thr Arg Cys Pro Thr Gln Gly Glu Pro
65                  70                  75                  80  
Tyr Leu Lys Glu Glu Gln Asp Gln Gln Tyr Ile Cys Arg Arg Asp Val
                85                  90                  95      
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Gly
            100                 105                 110         
Val Val Thr Cys Ala Lys Phe Ser Cys Ser Gly Lys Ile Thr Gly Asn
        115                 120                 125             
Leu Val Gln Ile Glu Asn Leu Glu Tyr Thr Val Val Val Thr Val His
    130                 135                 140                 
Asn Gly Asp Thr His Ala Val Gly Asn Asp Thr Ser Asn His Gly Val
145                 150                 155                 160 
Thr Ala Thr Ile Thr Pro Arg Ser Pro Ser Val Glu Val Lys Leu Pro
                165                 170                 175     
Asp Tyr Gly Glu Leu Thr Leu Asp Cys Glu Pro Arg Ser Gly Ile Asp
            180                 185                 190         
Phe Asn Glu Met Ile Leu Met Lys Met Lys Lys Lys Thr Trp Leu Val
        195                 200                 205             
His Lys Gln Trp Phe Leu Asp Leu Pro Leu Pro Trp Ala Ala Gly Ala
    210                 215                 220                 
Asp Thr Ser Glu Val His Trp Asn Tyr Lys Glu Arg Met Val Thr Phe
225                 230                 235                 240 
Lys Val Pro His Ala Lys Arg Gln Asp Val Thr Val Leu Gly Ser Gln
                245                 250                 255     
Glu Gly Ala Met His Ser Ala Leu Thr Gly Ala Thr Glu Val Asp Ser
            260                 265                 270         
Gly Asp Gly Asn His Met Tyr Ala Gly His Leu Lys Cys Lys Val Arg
        275                 280                 285             
Met Glu Lys Leu Arg Ile Lys Gly Met Ser Tyr Thr Met Cys Ser Gly
    290                 295                 300                 
Lys Phe Ser Ile Asp Lys Glu Met Ala Glu Thr Gln His Gly Thr Thr
305                 310                 315                 320 
Val Val Lys Val Lys Tyr Glu Gly Ala Gly Ala Pro Cys Lys Val Pro
                325                 330                 335     
Ile Glu Ile Arg Asp Val Asn Lys Glu Lys Val Val Gly Arg Ile Ile
            340                 345                 350         
Ser Ser Thr Pro Phe Ala Glu Tyr Thr Asn Ser Val Thr Asn Ile Glu
        355                 360                 365             
Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp
    370                 375                 380                 
Ser Ala Leu Thr Leu His Trp Phe Arg Lys Gly
385                 390                 395 


<210> 10
<211> 451
<212> PRT
<213> Artificial Sequence

<220> 
<223> DEN4-80EZip

<400> 10
Met Arg Cys Val Gly Val Gly Asn Arg Asp Phe Val Glu Gly Val Ser
 1               5                  10                  15      
Gly Gly Ala Trp Val Asp Leu Val Leu Glu His Gly Gly Cys Val Thr
            20                  25                  30          
Thr Met Ala Gln Gly Lys Pro Thr Leu Asp Phe Glu Leu Ile Lys Thr
        35                  40                  45              
Thr Ala Lys Glu Val Ala Leu Leu Arg Thr Tyr Cys Ile Glu Ala Ser
    50                  55                  60                  
Ile Ser Asn Ile Thr Thr Ala Thr Arg Cys Pro Thr Gln Gly Glu Pro
65                  70                  75                  80  
Tyr Leu Lys Glu Glu Gln Asp Gln Gln Tyr Ile Cys Arg Arg Asp Val
                85                  90                  95      
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Gly
            100                 105                 110         
Val Val Thr Cys Ala Lys Phe Ser Cys Ser Gly Lys Ile Thr Gly Asn
        115                 120                 125             
Leu Val Gln Ile Glu Asn Leu Glu Tyr Thr Val Val Val Thr Val His
    130                 135                 140                 
Asn Gly Asp Thr His Ala Val Gly Asn Asp Thr Ser Asn His Gly Val
145                 150                 155                 160 
Thr Ala Thr Ile Thr Pro Arg Ser Pro Ser Val Glu Val Lys Leu Pro
                165                 170                 175     
Asp Tyr Gly Glu Leu Thr Leu Asp Cys Glu Pro Arg Ser Gly Ile Asp
            180                 185                 190         
Phe Asn Glu Met Ile Leu Met Lys Met Lys Lys Lys Thr Trp Leu Val
        195                 200                 205             
His Lys Gln Trp Phe Leu Asp Leu Pro Leu Pro Trp Ala Ala Gly Ala
    210                 215                 220                 
Asp Thr Ser Glu Val His Trp Asn Tyr Lys Glu Arg Met Val Thr Phe
225                 230                 235                 240 
Lys Val Pro His Ala Lys Arg Gln Asp Val Thr Val Leu Gly Ser Gln
                245                 250                 255     
Glu Gly Ala Met His Ser Ala Leu Thr Gly Ala Thr Glu Val Asp Ser
            260                 265                 270         
Gly Asp Gly Asn His Met Tyr Ala Gly His Leu Lys Cys Lys Val Arg
        275                 280                 285             
Met Glu Lys Leu Arg Ile Lys Gly Met Ser Tyr Thr Met Cys Ser Gly
    290                 295                 300                 
Lys Phe Ser Ile Asp Lys Glu Met Ala Glu Thr Gln His Gly Thr Thr
305                 310                 315                 320 
Val Val Lys Val Lys Tyr Glu Gly Ala Gly Ala Pro Cys Lys Val Pro
                325                 330                 335     
Ile Glu Ile Arg Asp Val Asn Lys Glu Lys Val Val Gly Arg Ile Ile
            340                 345                 350         
Ser Ser Thr Pro Phe Ala Glu Tyr Thr Asn Ser Val Thr Asn Ile Glu
        355                 360                 365             
Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp
    370                 375                 380                 
Ser Ala Leu Thr Leu His Trp Phe Arg Lys Gly Gly Gly Gly Ser Gly
385                 390                 395                 400 
Gly Gly Gly Thr Gly Gly Gly Ser Gly Gly Gly Ser Pro Arg Met Lys
                405                 410                 415     
Gln Leu Glu Asp Lys Val Glu Glu Leu Leu Ser Lys Asn Tyr His Leu
            420                 425                 430         
Glu Asn Glu Val Ala Arg Leu Lys Lys Leu Val Gly Glu Arg Gly Gly
        435                 440                 445             
Cys Gly Gly
    450     

