                         SEQUENCE LISTING

<110>  Regents of the University of Colorado, a body corporate
       Konkuk University
       Omni Bio Pharmaceutical, Inc.
       Dinarello, Charles A.
       Kim, SooHyun
       Crapo, James D.
 
<120>  COMPOSITIONS, METHODS AND USES FOR ALPHA-1 ANTITRYPSIN FUSION 
       MOLECULES

<130>  479487.2

<150>  61/500,795
<151>  2011-06-24

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  394
<212>  PRT
<213>  Homo Sapiens

<400>  1

Glu Asp Pro Gln Gly Asp Ala Ala Gln Lys Thr Asp Thr Ser His His 
1               5                   10                  15      


Asp Gln Asp His Pro Thr Phe Asn Lys Ile Thr Pro Asn Leu Ala Glu 
            20                  25                  30          


Phe Ala Phe Ser Leu Tyr Arg Gln Leu Ala His Gln Ser Asn Ser Thr 
        35                  40                  45              


Asn Ile Phe Phe Ser Pro Val Ser Ile Ala Thr Ala Phe Ala Met Leu 
    50                  55                  60                  


Ser Leu Gly Thr Lys Ala Asp Thr His Asp Glu Ile Leu Glu Gly Leu 
65                  70                  75                  80  


Asn Phe Asn Leu Thr Glu Ile Pro Glu Ala Gln Ile His Glu Gly Phe 
                85                  90                  95      


Gln Glu Leu Leu Arg Thr Leu Asn Gln Pro Asp Ser Gln Leu Gln Leu 
            100                 105                 110         


Thr Thr Gly Asn Gly Leu Phe Leu Ser Glu Gly Leu Lys Leu Val Asp 
        115                 120                 125             


Lys Phe Leu Glu Asp Val Lys Lys Leu Tyr His Ser Glu Ala Phe Thr 
    130                 135                 140                 


Val Asn Phe Gly Asp Thr Glu Glu Ala Lys Lys Gln Ile Asn Asp Tyr 
145                 150                 155                 160 


Val Glu Lys Gly Thr Gln Gly Lys Ile Val Asp Leu Val Lys Glu Leu 
                165                 170                 175     


Asp Arg Asp Thr Val Phe Ala Leu Val Asn Tyr Ile Phe Phe Lys Gly 
            180                 185                 190         


Lys Trp Glu Arg Pro Phe Glu Val Lys Asp Thr Glu Glu Glu Asp Phe 
        195                 200                 205             


His Val Asp Gln Val Thr Thr Val Lys Val Pro Met Met Lys Arg Leu 
    210                 215                 220                 


Gly Met Phe Asn Ile Gln His Cys Lys Lys Leu Ser Ser Trp Val Leu 
225                 230                 235                 240 


Leu Met Lys Tyr Leu Gly Asn Ala Thr Ala Ile Phe Phe Leu Pro Asp 
                245                 250                 255     


Glu Gly Lys Leu Gln His Leu Glu Asn Glu Leu Thr His Asp Ile Ile 
            260                 265                 270         


Thr Lys Phe Leu Glu Asn Glu Asp Arg Arg Ser Ala Ser Leu His Leu 
        275                 280                 285             


Pro Lys Leu Ser Ile Thr Gly Thr Tyr Asp Leu Lys Ser Val Leu Gly 
    290                 295                 300                 


Gln Leu Gly Ile Thr Lys Val Phe Ser Asn Gly Ala Asp Leu Ser Gly 
305                 310                 315                 320 


Val Thr Glu Glu Ala Pro Leu Lys Leu Ser Lys Ala Val His Lys Ala 
                325                 330                 335     


Val Leu Thr Ile Asp Glu Lys Gly Thr Glu Ala Ala Gly Ala Met Phe 
            340                 345                 350         


Leu Glu Ala Ile Pro Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys 
        355                 360                 365             


Pro Phe Val Phe Leu Met Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe 
    370                 375                 380                 


Met Gly Lys Val Val Asn Pro Thr Gln Lys 
385                 390                 


<210>  2
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  2

Phe Val Phe Leu Met 
1               5   


<210>  3
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  3

Phe Val Phe Ala Met 
1               5   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  4

Phe Val Ala Leu Met 
1               5   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  5

Phe Val Phe Leu Ala 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  6

Phe Leu Val Phe Ile 
1               5   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  7

Phe Leu Met Ile Ile 
1               5   


<210>  8
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  8

Phe Leu Phe Val Leu 
1               5   


<210>  9
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  9

Phe Leu Phe Val Val 
1               5   


<210>  10
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  10

Phe Leu Phe Leu Ile 
1               5   


<210>  11
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  11

Phe Leu Phe Phe Ile 
1               5   


<210>  12
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  12

Phe Leu Met Phe Ile 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  13

Phe Met Leu Leu Ile 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  14

Phe Ile Ile Met Ile 
1               5   


<210>  15
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  15

Phe Leu Phe Cys Ile 
1               5   


<210>  16
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  16

Phe Leu Phe Ala Val 
1               5   


<210>  17
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  17

Phe Val Tyr Leu Ile 
1               5   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  18

Phe Ala Phe Leu Met 
1               5   


<210>  19
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  19

Ala Val Phe Leu Met 
1               5   


<210>  20
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  20

Gly Ile Thr Lys Val Phe Ser Asn Gly Ala 
1               5                   10  


<210>  21
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  21

Asp Leu Ser Gly Val Thr Glu Glu Ala Pro 
1               5                   10  


<210>  22
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  22

Leu Lys Leu Ser Lys Ala Val His Lys Ala 
1               5                   10  


<210>  23
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  23

Val Leu Thr Ile Asp Glu Lys Gly Thr Glu 
1               5                   10  


<210>  24
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  24

Ala Ala Gly Ala Met Phe Leu Glu Ala Ile 
1               5                   10  


<210>  25
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  25

Pro Met Ser Ile Pro Pro Glu Val Lys Phe 
1               5                   10  


<210>  26
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  26

Asn Lys Pro Phe Val Phe Leu Met Ile Glu 
1               5                   10  


<210>  27
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  27

Gln Asn Thr Lys Ser Pro Leu Phe Met Gly 
1               5                   10  


<210>  28
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  28

Lys Val Val Asn Pro Thr Gln Lys 
1               5               


<210>  29
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  29

Leu Glu Ala Ile Pro Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys 
1               5                   10                  15      


Pro Phe Val Phe Leu Met 
            20          


<210>  30
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide

<400>  30

Leu Glu Ala Ile Pro Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys 
1               5                   10                  15      


Pro Phe Val Phe 
            20  


<210>  31
<211>  80
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  31

Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu Lys Leu Ser 
1               5                   10                  15      


Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys Gly Thr Glu 
            20                  25                  30          


Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser Ile Pro Pro 
        35                  40                  45              


Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile Glu Gln Asn 
    50                  55                  60                  


Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro Thr Gln Lys 
65                  70                  75                  80  


<210>  32
<211>  652
<212>  PRT
<213>  Artificial

<220>
<223>  FC-AAT fusion polypeptide with linker and leader sequence


<220>
<221>  linker between immune molecule and AAT
<222>  (419)..(420)

<400>  32

Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys 
1               5                   10                  15      


Cys Leu Val Pro Val Ser Leu Ala Glu Asp Pro Gln Gly Asp Ala Ala 
            20                  25                  30          


Gln Lys Thr Asp Thr Ser His His Asp Gln Asp His Pro Thr Phe Asn 
        35                  40                  45              


Lys Ile Thr Pro Asn Leu Ala Glu Phe Ala Phe Ser Leu Tyr Arg Gln 
    50                  55                  60                  


Leu Ala His Gln Ser Asn Ser Thr Asn Ile Phe Phe Ser Pro Val Ser 
65                  70                  75                  80  


Ile Ala Thr Ala Phe Ala Met Leu Ser Leu Gly Thr Lys Ala Asp Thr 
                85                  90                  95      


His Asp Glu Ile Leu Glu Gly Leu Asn Phe Asn Leu Thr Glu Ile Pro 
            100                 105                 110         


Glu Ala Gln Ile His Glu Gly Phe Gln Glu Leu Leu Arg Thr Leu Asn 
        115                 120                 125             


Gln Pro Asp Ser Gln Leu Gln Leu Thr Thr Gly Asn Gly Leu Phe Leu 
    130                 135                 140                 


Ser Glu Gly Leu Lys Leu Val Asp Lys Phe Leu Glu Asp Val Lys Lys 
145                 150                 155                 160 


Leu Tyr His Ser Glu Ala Phe Thr Val Asn Phe Gly Asp Thr Glu Glu 
                165                 170                 175     


Ala Lys Lys Gln Ile Asn Asp Tyr Val Glu Lys Gly Thr Gln Gly Lys 
            180                 185                 190         


Ile Val Asp Leu Val Lys Glu Leu Asp Arg Asp Thr Val Phe Ala Leu 
        195                 200                 205             


Val Asn Tyr Ile Phe Phe Lys Gly Lys Trp Glu Arg Pro Phe Glu Val 
    210                 215                 220                 


Lys Asp Thr Glu Glu Glu Asp Phe His Val Asp Gln Ala Thr Thr Val 
225                 230                 235                 240 


Lys Val Pro Met Met Lys Arg Leu Gly Met Phe Asn Ile Gln His Cys 
                245                 250                 255     


Lys Lys Leu Ser Ser Trp Val Leu Leu Met Lys Tyr Leu Gly Asn Ala 
            260                 265                 270         


Thr Ala Ile Phe Phe Leu Pro Asp Glu Gly Lys Leu Gln His Leu Glu 
        275                 280                 285             


Asn Glu Leu Thr His Asp Ile Ile Thr Lys Phe Leu Glu Asn Glu Asp 
    290                 295                 300                 


Arg Arg Ser Ala Ser Leu His Leu Pro Lys Leu Ser Ile Thr Gly Thr 
305                 310                 315                 320 


Tyr Asp Leu Lys Ser Val Leu Gly Gln Leu Gly Ile Thr Lys Val Phe 
                325                 330                 335     


Ser Asn Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu Lys 
            340                 345                 350         


Leu Ser Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys Gly 
        355                 360                 365             


Thr Glu Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser Ile 
    370                 375                 380                 


Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile Glu 
385                 390                 395                 400 


Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro Thr 
                405                 410                 415     


Gln Lys Thr Arg Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
            420                 425                 430         


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
        435                 440                 445             


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
    450                 455                 460                 


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
465                 470                 475                 480 


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
                485                 490                 495     


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
            500                 505                 510         


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
        515                 520                 525             


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
    530                 535                 540                 


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
545                 550                 555                 560 


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
                565                 570                 575     


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
            580                 585                 590         


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
        595                 600                 605             


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
    610                 615                 620                 


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
625                 630                 635                 640 


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                645                 650         


<210>  33
<211>  394
<212>  PRT
<213>  Homo Sapiens

<400>  33

Glu Asp Pro Gln Gly Asp Ala Ala Gln Lys Thr Asp Thr Ser His His 
1               5                   10                  15      


Asp Gln Asp His Pro Thr Phe Asn Lys Ile Thr Pro Asn Leu Ala Glu 
            20                  25                  30          


Phe Ala Phe Ser Leu Tyr Arg Gln Leu Ala His Gln Ser Asn Ser Thr 
        35                  40                  45              


Asn Ile Phe Phe Ser Pro Val Ser Ile Ala Thr Ala Phe Ala Met Leu 
    50                  55                  60                  


Ser Leu Gly Thr Lys Ala Asp Thr His Asp Glu Ile Leu Glu Gly Leu 
65                  70                  75                  80  


Asn Phe Asn Leu Thr Glu Ile Pro Glu Ala Gln Ile His Glu Gly Phe 
                85                  90                  95      


Gln Glu Leu Leu Arg Thr Leu Asn Gln Pro Asp Ser Gln Leu Gln Leu 
            100                 105                 110         


Thr Thr Gly Asn Gly Leu Phe Leu Ser Glu Gly Leu Lys Leu Val Asp 
        115                 120                 125             


Lys Phe Leu Glu Asp Val Lys Lys Leu Tyr His Ser Glu Ala Phe Thr 
    130                 135                 140                 


Val Asn Phe Gly Asp Thr Glu Glu Ala Lys Lys Gln Ile Asn Asp Tyr 
145                 150                 155                 160 


Val Glu Lys Gly Thr Gln Gly Lys Ile Val Asp Leu Val Lys Glu Leu 
                165                 170                 175     


Asp Arg Asp Thr Val Phe Ala Leu Val Asn Tyr Ile Phe Phe Lys Gly 
            180                 185                 190         


Lys Trp Glu Arg Pro Phe Glu Val Lys Asp Thr Glu Glu Glu Asp Phe 
        195                 200                 205             


His Val Asp Gln Ala Thr Thr Val Lys Val Pro Met Met Lys Arg Leu 
    210                 215                 220                 


Gly Met Phe Asn Ile Gln His Cys Lys Lys Leu Ser Ser Trp Val Leu 
225                 230                 235                 240 


Leu Met Lys Tyr Leu Gly Asn Ala Thr Ala Ile Phe Phe Leu Pro Asp 
                245                 250                 255     


Glu Gly Lys Leu Gln His Leu Glu Asn Glu Leu Thr His Asp Ile Ile 
            260                 265                 270         


Thr Lys Phe Leu Glu Asn Glu Asp Arg Arg Ser Ala Ser Leu His Leu 
        275                 280                 285             


Pro Lys Leu Ser Ile Thr Gly Thr Tyr Asp Leu Lys Ser Val Leu Gly 
    290                 295                 300                 


Gln Leu Gly Ile Thr Lys Val Phe Ser Asn Gly Ala Asp Leu Ser Gly 
305                 310                 315                 320 


Val Thr Glu Glu Ala Pro Leu Lys Leu Ser Lys Ala Val His Lys Ala 
                325                 330                 335     


Val Leu Thr Ile Asp Glu Lys Gly Thr Glu Ala Ala Gly Ala Met Phe 
            340                 345                 350         


Leu Glu Ala Ile Pro Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys 
        355                 360                 365             


Pro Phe Val Phe Leu Met Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe 
    370                 375                 380                 


Met Gly Lys Val Val Asn Pro Thr Gln Lys 
385                 390                 


<210>  34
<211>  36
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  34

Ser Ile Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met 
1               5                   10                  15      


Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn 
            20                  25                  30          


Pro Thr Gln Lys 
        35      


<210>  35
<211>  62
<212>  DNA
<213>  Homo sapiens

<400>  35
tttagaggcc atacccatgt ctatcccccc cgaggtcaag ttcaacaaac ccctttgtct       60

tt                                                                      62


<210>  36
<211>  62
<212>  DNA
<213>  Artificial

<220>
<223>  mutant

<400>  36
tttagaggcc atatgcatgt ctatcccccc cgaggtcaag ttcaacaaac ccctttgtct       60

tt                                                                      62


<210>  37
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  37

Ala Ile Pro Arg Ser Ile Pro Pro Glu 
1               5                   


<210>  38
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  38

Ala Ile Pro Val Ser Ile Pro Pro Glu 
1               5                   


<210>  39
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  syntehtic peptide

<400>  39

Ala Ile Pro Val Ser Ile Pro Pro Glu 
1               5                   


<210>  40
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  40

Ala Ile Pro Leu Ser Ile Pro Pro Glu 
1               5                   


<210>  41
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  41

Ala Ile Cys Met Ser Ile Pro Pro Glu 
1               5                   


<210>  42
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  syntehtic peptide

<400>  42

Ala Ile Pro Ala Ser Ile Pro Pro Glu 
1               5                   


<210>  43
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  43

Ala Ile Pro Met Ser Ile Pro Pro Lys 
1               5                   


<210>  44
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  44

Ala Ala Gly Arg Ser Leu Asn Pro Glu 
1               5                   


<210>  45
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  45

Ile Ala Gly Arg Ser Leu Asn Pro Asn 
1               5                   


<210>  46
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic peptide

<400>  46

Ile Ala Gly Arg Leu Leu Asn Pro Asn 
1               5                   


<210>  47
<211>  650
<212>  PRT
<213>  Artificial

<220>
<223>  Fc-AAT with leader sequence without linker

<400>  47

Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys 
1               5                   10                  15      


Cys Leu Val Pro Val Ser Leu Ala Glu Asp Pro Gln Gly Asp Ala Ala 
            20                  25                  30          


Gln Lys Thr Asp Thr Ser His His Asp Gln Asp His Pro Thr Phe Asn 
        35                  40                  45              


Lys Ile Thr Pro Asn Leu Ala Glu Phe Ala Phe Ser Leu Tyr Arg Gln 
    50                  55                  60                  


Leu Ala His Gln Ser Asn Ser Thr Asn Ile Phe Phe Ser Pro Val Ser 
65                  70                  75                  80  


Ile Ala Thr Ala Phe Ala Met Leu Ser Leu Gly Thr Lys Ala Asp Thr 
                85                  90                  95      


His Asp Glu Ile Leu Glu Gly Leu Asn Phe Asn Leu Thr Glu Ile Pro 
            100                 105                 110         


Glu Ala Gln Ile His Glu Gly Phe Gln Glu Leu Leu Arg Thr Leu Asn 
        115                 120                 125             


Gln Pro Asp Ser Gln Leu Gln Leu Thr Thr Gly Asn Gly Leu Phe Leu 
    130                 135                 140                 


Ser Glu Gly Leu Lys Leu Val Asp Lys Phe Leu Glu Asp Val Lys Lys 
145                 150                 155                 160 


Leu Tyr His Ser Glu Ala Phe Thr Val Asn Phe Gly Asp Thr Glu Glu 
                165                 170                 175     


Ala Lys Lys Gln Ile Asn Asp Tyr Val Glu Lys Gly Thr Gln Gly Lys 
            180                 185                 190         


Ile Val Asp Leu Val Lys Glu Leu Asp Arg Asp Thr Val Phe Ala Leu 
        195                 200                 205             


Val Asn Tyr Ile Phe Phe Lys Gly Lys Trp Glu Arg Pro Phe Glu Val 
    210                 215                 220                 


Lys Asp Thr Glu Glu Glu Asp Phe His Val Asp Gln Ala Thr Thr Val 
225                 230                 235                 240 


Lys Val Pro Met Met Lys Arg Leu Gly Met Phe Asn Ile Gln His Cys 
                245                 250                 255     


Lys Lys Leu Ser Ser Trp Val Leu Leu Met Lys Tyr Leu Gly Asn Ala 
            260                 265                 270         


Thr Ala Ile Phe Phe Leu Pro Asp Glu Gly Lys Leu Gln His Leu Glu 
        275                 280                 285             


Asn Glu Leu Thr His Asp Ile Ile Thr Lys Phe Leu Glu Asn Glu Asp 
    290                 295                 300                 


Arg Arg Ser Ala Ser Leu His Leu Pro Lys Leu Ser Ile Thr Gly Thr 
305                 310                 315                 320 


Tyr Asp Leu Lys Ser Val Leu Gly Gln Leu Gly Ile Thr Lys Val Phe 
                325                 330                 335     


Ser Asn Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu Lys 
            340                 345                 350         


Leu Ser Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys Gly 
        355                 360                 365             


Thr Glu Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser Ile 
    370                 375                 380                 


Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile Glu 
385                 390                 395                 400 


Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro Thr 
                405                 410                 415     


Gln Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            420                 425                 430         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        435                 440                 445             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    450                 455                 460                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
465                 470                 475                 480 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                485                 490                 495     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            500                 505                 510         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        515                 520                 525             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    530                 535                 540                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
545                 550                 555                 560 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                565                 570                 575     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            580                 585                 590         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        595                 600                 605             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    610                 615                 620                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
625                 630                 635                 640 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                645                 650 


<210>  48
<211>  628
<212>  PRT
<213>  Artificial

<220>
<223>  Fc-AAT fusion polypeptide with linker without leader sequence


<220>
<221>  linker between immune molecule and AAT
<222>  (395)..(396)

<400>  48

Glu Asp Pro Gln Gly Asp Ala Ala Gln Lys Thr Asp Thr Ser His His 
1               5                   10                  15      


Asp Gln Asp His Pro Thr Phe Asn Lys Ile Thr Pro Asn Leu Ala Glu 
            20                  25                  30          


Phe Ala Phe Ser Leu Tyr Arg Gln Leu Ala His Gln Ser Asn Ser Thr 
        35                  40                  45              


Asn Ile Phe Phe Ser Pro Val Ser Ile Ala Thr Ala Phe Ala Met Leu 
    50                  55                  60                  


Ser Leu Gly Thr Lys Ala Asp Thr His Asp Glu Ile Leu Glu Gly Leu 
65                  70                  75                  80  


Asn Phe Asn Leu Thr Glu Ile Pro Glu Ala Gln Ile His Glu Gly Phe 
                85                  90                  95      


Gln Glu Leu Leu Arg Thr Leu Asn Gln Pro Asp Ser Gln Leu Gln Leu 
            100                 105                 110         


Thr Thr Gly Asn Gly Leu Phe Leu Ser Glu Gly Leu Lys Leu Val Asp 
        115                 120                 125             


Lys Phe Leu Glu Asp Val Lys Lys Leu Tyr His Ser Glu Ala Phe Thr 
    130                 135                 140                 


Val Asn Phe Gly Asp Thr Glu Glu Ala Lys Lys Gln Ile Asn Asp Tyr 
145                 150                 155                 160 


Val Glu Lys Gly Thr Gln Gly Lys Ile Val Asp Leu Val Lys Glu Leu 
                165                 170                 175     


Asp Arg Asp Thr Val Phe Ala Leu Val Asn Tyr Ile Phe Phe Lys Gly 
            180                 185                 190         


Lys Trp Glu Arg Pro Phe Glu Val Lys Asp Thr Glu Glu Glu Asp Phe 
        195                 200                 205             


His Val Asp Gln Ala Thr Thr Val Lys Val Pro Met Met Lys Arg Leu 
    210                 215                 220                 


Gly Met Phe Asn Ile Gln His Cys Lys Lys Leu Ser Ser Trp Val Leu 
225                 230                 235                 240 


Leu Met Lys Tyr Leu Gly Asn Ala Thr Ala Ile Phe Phe Leu Pro Asp 
                245                 250                 255     


Glu Gly Lys Leu Gln His Leu Glu Asn Glu Leu Thr His Asp Ile Ile 
            260                 265                 270         


Thr Lys Phe Leu Glu Asn Glu Asp Arg Arg Ser Ala Ser Leu His Leu 
        275                 280                 285             


Pro Lys Leu Ser Ile Thr Gly Thr Tyr Asp Leu Lys Ser Val Leu Gly 
    290                 295                 300                 


Gln Leu Gly Ile Thr Lys Val Phe Ser Asn Gly Ala Asp Leu Ser Gly 
305                 310                 315                 320 


Val Thr Glu Glu Ala Pro Leu Lys Leu Ser Lys Ala Val His Lys Ala 
                325                 330                 335     


Val Leu Thr Ile Asp Glu Lys Gly Thr Glu Ala Ala Gly Ala Met Phe 
            340                 345                 350         


Leu Glu Ala Ile Pro Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys 
        355                 360                 365             


Pro Phe Val Phe Leu Met Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe 
    370                 375                 380                 


Met Gly Lys Val Val Asn Pro Thr Gln Lys Thr Arg Glu Pro Lys Ser 
385                 390                 395                 400 


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
                405                 410                 415     


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            420                 425                 430         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        435                 440                 445             


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    450                 455                 460                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
465                 470                 475                 480 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                485                 490                 495     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
            500                 505                 510         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        515                 520                 525             


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
    530                 535                 540                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
545                 550                 555                 560 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                565                 570                 575     


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            580                 585                 590         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        595                 600                 605             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    610                 615                 620                 


Ser Pro Gly Lys 
625             


<210>  49
<211>  626
<212>  PRT
<213>  Artificial

<220>
<223>  Fc-AAT fusion polypeptide without linker or leader sequence

<400>  49

Glu Asp Pro Gln Gly Asp Ala Ala Gln Lys Thr Asp Thr Ser His His 
1               5                   10                  15      


Asp Gln Asp His Pro Thr Phe Asn Lys Ile Thr Pro Asn Leu Ala Glu 
            20                  25                  30          


Phe Ala Phe Ser Leu Tyr Arg Gln Leu Ala His Gln Ser Asn Ser Thr 
        35                  40                  45              


Asn Ile Phe Phe Ser Pro Val Ser Ile Ala Thr Ala Phe Ala Met Leu 
    50                  55                  60                  


Ser Leu Gly Thr Lys Ala Asp Thr His Asp Glu Ile Leu Glu Gly Leu 
65                  70                  75                  80  


Asn Phe Asn Leu Thr Glu Ile Pro Glu Ala Gln Ile His Glu Gly Phe 
                85                  90                  95      


Gln Glu Leu Leu Arg Thr Leu Asn Gln Pro Asp Ser Gln Leu Gln Leu 
            100                 105                 110         


Thr Thr Gly Asn Gly Leu Phe Leu Ser Glu Gly Leu Lys Leu Val Asp 
        115                 120                 125             


Lys Phe Leu Glu Asp Val Lys Lys Leu Tyr His Ser Glu Ala Phe Thr 
    130                 135                 140                 


Val Asn Phe Gly Asp Thr Glu Glu Ala Lys Lys Gln Ile Asn Asp Tyr 
145                 150                 155                 160 


Val Glu Lys Gly Thr Gln Gly Lys Ile Val Asp Leu Val Lys Glu Leu 
                165                 170                 175     


Asp Arg Asp Thr Val Phe Ala Leu Val Asn Tyr Ile Phe Phe Lys Gly 
            180                 185                 190         


Lys Trp Glu Arg Pro Phe Glu Val Lys Asp Thr Glu Glu Glu Asp Phe 
        195                 200                 205             


His Val Asp Gln Ala Thr Thr Val Lys Val Pro Met Met Lys Arg Leu 
    210                 215                 220                 


Gly Met Phe Asn Ile Gln His Cys Lys Lys Leu Ser Ser Trp Val Leu 
225                 230                 235                 240 


Leu Met Lys Tyr Leu Gly Asn Ala Thr Ala Ile Phe Phe Leu Pro Asp 
                245                 250                 255     


Glu Gly Lys Leu Gln His Leu Glu Asn Glu Leu Thr His Asp Ile Ile 
            260                 265                 270         


Thr Lys Phe Leu Glu Asn Glu Asp Arg Arg Ser Ala Ser Leu His Leu 
        275                 280                 285             


Pro Lys Leu Ser Ile Thr Gly Thr Tyr Asp Leu Lys Ser Val Leu Gly 
    290                 295                 300                 


Gln Leu Gly Ile Thr Lys Val Phe Ser Asn Gly Ala Asp Leu Ser Gly 
305                 310                 315                 320 


Val Thr Glu Glu Ala Pro Leu Lys Leu Ser Lys Ala Val His Lys Ala 
                325                 330                 335     


Val Leu Thr Ile Asp Glu Lys Gly Thr Glu Ala Ala Gly Ala Met Phe 
            340                 345                 350         


Leu Glu Ala Ile Pro Met Ser Ile Pro Pro Glu Val Lys Phe Asn Lys 
        355                 360                 365             


Pro Phe Val Phe Leu Met Ile Glu Gln Asn Thr Lys Ser Pro Leu Phe 
    370                 375                 380                 


Met Gly Lys Val Val Asn Pro Thr Gln Lys Glu Pro Lys Ser Cys Asp 
385                 390                 395                 400 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                405                 410                 415     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            420                 425                 430         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        435                 440                 445             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    450                 455                 460                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
465                 470                 475                 480 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                485                 490                 495     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            500                 505                 510         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        515                 520                 525             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    530                 535                 540                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
545                 550                 555                 560 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                565                 570                 575     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            580                 585                 590         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        595                 600                 605             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    610                 615                 620                 


Gly Lys 
625     


<210>  50
<211>  1887
<212>  DNA
<213>  Artificial

<220>
<223>  cDNA for Fc-AAT with linker without leader sequence

<400>  50
gaggatcccc agggagatgc tgcccagaag acagatacat cccaccacga tcaggatcac       60

ccaaccttca acaagatcac ccccaacctg gctgagttcg ccttcagcct ataccgccag      120

ctggcacacc agtccaacag caccaatatc ttcttctccc cagtgagcat cgctacagcc      180

tttgcaatgc tctccctggg gaccaaggct gacactcacg atgaaatcct ggagggcctg      240

aatttcaacc tcacggagat tccggaggct cagatccatg aaggcttcca ggaactcctc      300

cgtaccctca accagccaga cagccagctc cagctgacca ccggcaatgg cctgttcctc      360

agcgagggcc tgaagctagt ggataagttt ttggaggatg ttaaaaagtt gtaccactca      420

gaagccttca ctgtcaactt cggggacacc gaagaggcca agaaacagat caacgattac      480

gtggagaagg gtactcaagg gaaaattgtg gatttggtca aggagcttga cagagacaca      540

gtttttgctc tggtgaatta catcttcttt aaaggcaaat gggagagacc ctttgaagtc      600

aaggacaccg aggaagagga cttccacgtg gaccaggcga ccaccgtgaa ggtgcctatg      660

atgaagcgtt taggcatgtt taacatccag cactgtaaga agctgtccag ctgggtgctg      720

ctgatgaaat acctgggcaa tgccaccgcc atcttcttcc tgcctgatga ggggaaacta      780

cagcacctgg aaaatgaact cacccacgat atcatcacca agttcctgga aaatgaagac      840

agaaggtctg ccagcttaca tttacccaaa ctgtccatta ctggaaccta tgatctgaag      900

agcgtcctgg gtcaactggg catcactaag gtcttcagca atggggctga cctctccggg      960

gtcacagagg aggcacccct gaagctctcc aaggccgtgc ataaggctgt gctgaccatc     1020

gacgagaaag ggactgaagc tgctggggcc atgtttttag aggccatacc catgtctatc     1080

ccccccgagg tcaagttcaa caaacccttt gtcttcttaa tgattgaaca aaataccaag     1140

tctcccctct tcatgggaaa agtggtgaat cccacccaaa aaacgcgtga gcccaaatct     1200

tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca     1260

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc     1320

acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg     1380

gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg     1440

taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac     1500

aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc     1560

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc     1620

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg     1680

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac     1740

tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag     1800

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1860

agcctctccc tgtctccggg taaatga                                         1887


<210>  51
<211>  1959
<212>  DNA
<213>  Artificial

<220>
<223>  cDNA for Fc-AAT with linker and leader sequence

<400>  51
atgccgtctt ctgtctcgtg gggcatcctc ctgctggcag gcctgtgctg cctggtccct       60

gtctccctgg ctgaggatcc ccagggagat gctgcccaga agacagatac atcccaccac      120

gatcaggatc acccaacctt caacaagatc acccccaacc tggctgagtt cgccttcagc      180

ctataccgcc agctggcaca ccagtccaac agcaccaata tcttcttctc cccagtgagc      240

atcgctacag cctttgcaat gctctccctg gggaccaagg ctgacactca cgatgaaatc      300

ctggagggcc tgaatttcaa cctcacggag attccggagg ctcagatcca tgaaggcttc      360

caggaactcc tccgtaccct caaccagcca gacagccagc tccagctgac caccggcaat      420

ggcctgttcc tcagcgaggg cctgaagcta gtggataagt ttttggagga tgttaaaaag      480

ttgtaccact cagaagcctt cactgtcaac ttcggggaca ccgaagaggc caagaaacag      540

atcaacgatt acgtggagaa gggtactcaa gggaaaattg tggatttggt caaggagctt      600

gacagagaca cagtttttgc tctggtgaat tacatcttct ttaaaggcaa atgggagaga      660

ccctttgaag tcaaggacac cgaggaagag gacttccacg tggaccaggc gaccaccgtg      720

aaggtgccta tgatgaagcg tttaggcatg tttaacatcc agcactgtaa gaagctgtcc      780

agctgggtgc tgctgatgaa atacctgggc aatgccaccg ccatcttctt cctgcctgat      840

gaggggaaac tacagcacct ggaaaatgaa ctcacccacg atatcatcac caagttcctg      900

gaaaatgaag acagaaggtc tgccagctta catttaccca aactgtccat tactggaacc      960

tatgatctga agagcgtcct gggtcaactg ggcatcacta aggtcttcag caatggggct     1020

gacctctccg gggtcacaga ggaggcaccc ctgaagctct ccaaggccgt gcataaggct     1080

gtgctgacca tcgacgagaa agggactgaa gctgctgggg ccatgttttt agaggccata     1140

cccatgtcta tcccccccga ggtcaagttc aacaaaccct ttgtcttctt aatgattgaa     1200

caaaatacca agtctcccct cttcatggga aaagtggtga atcccaccca aaaaacgcgt     1260

gagcccaaat cttgtgacaa aactcacaca tgcccaccgt gcccagcacc tgaactcctg     1320

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg     1380

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc     1440

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     1500

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     1560

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     1620

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg     1680

gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     1740

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     1800

cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt ggacaagagc     1860

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     1920

tacacgcaga agagcctctc cctgtctccg ggtaaatga                            1959


