                         SEQUENCE LISTING

<110>  University of Wyoming
       Geisler, Christoph
       Jarvis, Donald L
 
<120>  Use of bacterial GlcNAc-6-P 2-epimerase to facilitate 
       glycoconjugation

<130>  UWYO-D794U1 (UW 11-060)

<150>  US 61/513,254
<151>  2011-07-29

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic NanE forward Primer 1

<400>  1
gcggccgcac catgtcgtta cttgcacaac                                        30


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic NanE Reverse Primer 1

<400>  2
atgcggccgc tcatagcacc gcctttttc                                         29


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic NanE Forward Primer 2

<400>  3
gcggccgcac catgtcgtt                                                    19


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic NanE Reverse Primer 2

<400>  4
atgcggccgc tcatagcac                                                    19


<210>  5
<211>  711
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Synthetic region of amplified PCR product having a NotI 
       recognition site flanking 5' end of ORF encoding E. coli 
       GlcNAc-6-P 2' epimerase

<220>
<221>  CDS
<222>  (12)..(701)
<223>  Open Reading Frame (ORF) encoding E. coli K-12 substrain MG1655 
       GlcNAc-6-P 2' epimerase flanked by synthetic sequences having 
       recognition sites for NotI restriction endonuclease

<220>
<221>  misc_feature
<222>  (702)..(711)
<223>  Synthetic region of amplified PCR product having a NotI 
       recognition site flanking 3' end of ORF encoding E. coli 
       GlcNAc-6-P 2' epimerase

<400>  5
gcggccgcac c atg tcg tta ctt gca caa ttg gat caa aaa atc gct gct        50
             Met Ser Leu Leu Ala Gln Leu Asp Gln Lys Ile Ala Ala          
             1               5                   10                       

aac ggt ggc ctg att gtc tcc tgc cag ccg gtt ccg gac agc ccg ctc         98
Asn Gly Gly Leu Ile Val Ser Cys Gln Pro Val Pro Asp Ser Pro Leu           
    15                  20                  25                            

gat aaa ccc gaa atc gtc gcc gcc atg gca tta gcg gca gaa cag gcg        146
Asp Lys Pro Glu Ile Val Ala Ala Met Ala Leu Ala Ala Glu Gln Ala           
30                  35                  40                  45            

ggc gcg gtt gcc att cgc att gaa ggt gtg gca aat ctg caa gcc acg        194
Gly Ala Val Ala Ile Arg Ile Glu Gly Val Ala Asn Leu Gln Ala Thr           
                50                  55                  60                

cgt gcg gtg gtg agc gtg ccg att att gga att gtg aaa cgc gat ctg        242
Arg Ala Val Val Ser Val Pro Ile Ile Gly Ile Val Lys Arg Asp Leu           
            65                  70                  75                    

gag gat tct ccg gta cgc atc acg gcc tat att gaa gat gtt gat gcg        290
Glu Asp Ser Pro Val Arg Ile Thr Ala Tyr Ile Glu Asp Val Asp Ala           
        80                  85                  90                        

ctg gcg cag gcg ggc gcg gac att atc gcc att gac ggc acc gac cgc        338
Leu Ala Gln Ala Gly Ala Asp Ile Ile Ala Ile Asp Gly Thr Asp Arg           
    95                  100                 105                           

ccg cgt ccg gtg cct gtt gaa acg ctg ctg gca cgt att cac cat cac        386
Pro Arg Pro Val Pro Val Glu Thr Leu Leu Ala Arg Ile His His His           
110                 115                 120                 125           

ggt tta ctg gcg atg acc gac tgc tca acg ccg gaa gac ggc ctg gca        434
Gly Leu Leu Ala Met Thr Asp Cys Ser Thr Pro Glu Asp Gly Leu Ala           
                130                 135                 140               

tgc caa aag ctg gga gcc gaa att att ggc act acg ctt tct ggc tat        482
Cys Gln Lys Leu Gly Ala Glu Ile Ile Gly Thr Thr Leu Ser Gly Tyr           
            145                 150                 155                   

acc acg cct gaa acg cca gaa gag ccg gat ctg gcg ctg gtg aaa acg        530
Thr Thr Pro Glu Thr Pro Glu Glu Pro Asp Leu Ala Leu Val Lys Thr           
        160                 165                 170                       

ttg agc gac gcc gga tgt cgg gtg att gcc gaa ggg cgt tac aac acg        578
Leu Ser Asp Ala Gly Cys Arg Val Ile Ala Glu Gly Arg Tyr Asn Thr           
    175                 180                 185                           

cct gct cag gcg gcg gat gcg atg cgc cac ggc gcg tgg gcg gtg acg        626
Pro Ala Gln Ala Ala Asp Ala Met Arg His Gly Ala Trp Ala Val Thr           
190                 195                 200                 205           

gtc ggt tct gca atc acg cgt ctt gag cac att tgt cag tgg tac aac        674
Val Gly Ser Ala Ile Thr Arg Leu Glu His Ile Cys Gln Trp Tyr Asn           
                210                 215                 220               

aca gcg atg aaa aag gcg gtg cta tga gcggccgcat                         711
Thr Ala Met Lys Lys Ala Val Leu                                           
            225                                                           


<210>  6
<211>  229
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Ser Leu Leu Ala Gln Leu Asp Gln Lys Ile Ala Ala Asn Gly Gly 
1               5                   10                  15      


Leu Ile Val Ser Cys Gln Pro Val Pro Asp Ser Pro Leu Asp Lys Pro 
            20                  25                  30          


Glu Ile Val Ala Ala Met Ala Leu Ala Ala Glu Gln Ala Gly Ala Val 
        35                  40                  45              


Ala Ile Arg Ile Glu Gly Val Ala Asn Leu Gln Ala Thr Arg Ala Val 
    50                  55                  60                  


Val Ser Val Pro Ile Ile Gly Ile Val Lys Arg Asp Leu Glu Asp Ser 
65                  70                  75                  80  


Pro Val Arg Ile Thr Ala Tyr Ile Glu Asp Val Asp Ala Leu Ala Gln 
                85                  90                  95      


Ala Gly Ala Asp Ile Ile Ala Ile Asp Gly Thr Asp Arg Pro Arg Pro 
            100                 105                 110         


Val Pro Val Glu Thr Leu Leu Ala Arg Ile His His His Gly Leu Leu 
        115                 120                 125             


Ala Met Thr Asp Cys Ser Thr Pro Glu Asp Gly Leu Ala Cys Gln Lys 
    130                 135                 140                 


Leu Gly Ala Glu Ile Ile Gly Thr Thr Leu Ser Gly Tyr Thr Thr Pro 
145                 150                 155                 160 


Glu Thr Pro Glu Glu Pro Asp Leu Ala Leu Val Lys Thr Leu Ser Asp 
                165                 170                 175     


Ala Gly Cys Arg Val Ile Ala Glu Gly Arg Tyr Asn Thr Pro Ala Gln 
            180                 185                 190         


Ala Ala Asp Ala Met Arg His Gly Ala Trp Ala Val Thr Val Gly Ser 
        195                 200                 205             


Ala Ile Thr Arg Leu Glu His Ile Cys Gln Trp Tyr Asn Thr Ala Met 
    210                 215                 220                 


Lys Lys Ala Val Leu 
225                 


