                         SEQUENCE LISTING

<110>  Novozymes A/S
       Matsui, Tomoko
       Clark, Suzanne
 
<120>  GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

<130>  12191-WO-PCT

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1851
<212>  DNA
<213>  Penicillium oxalicum

<400>  1
atgcgtctca ctctattatc aggtgtagcc ggcgttctct gcgcaggaca gctgacggcg       60

gcgcgtcctg atcccaaggg tgggaatctg acgccgttca tccacaaaga gggcgagcgg      120

tcgctccaag gcatcttgga caatctcggt gggcgaggta agaaaacacc cggcactgcc      180

gcagggttgt ttattgccag tccaaacaca gagaatccaa actattatta tacatggact      240

cgtgactcag ctttgactgc caagtgcttg atcgacctgt tcgaagactc tcgggcaaag      300

tttccaattg accgcaaata cttggaaaca ggaattcggg actacgtgtc gtcccaagca      360

atcctccaga gtgtgtctaa tccttctgga accctgaagg atggctctgg tctgggtgaa      420

cccaagtttg agattgacct gaatcccttt tcgggtgcct ggggtcggcc tcagcgggat      480

ggcccagcgc tgcgagcgac cgctatgatc acctacgcca actacctgat atcccatggt      540

cagaaatcgg atgtgtcaca ggtcatgtgg ccgattattg ccaatgatct agcatatgtt      600

ggtcaatact ggaataatac cggatttgac ctgtgggaag aggtggatgg gtcaagcttt      660

ttcacgattg cggtccagca ccgagccctt gttgaaggct cgcaactggc gaaaaagctc      720

ggcaagtcct gcgatgcctg tgattctcag cctccccaga tattgtgttt cctgcagagt      780

ttctggaacg gaaagtacat cacctccaac atcaacacgc aagcaagccg ctctggtatc      840

gacctggact ctgtcctggg aagcattcat acctttgatc ccgaagcagc ctgtgacgat      900

gcaactttcc agccttgttc tgcccgcgct ctggcgaacc acaaggtcta tgtggattcc      960

ttccgctcta tctacaagat taatgcgggt cttgcagagg gatcggctgc caacgttggc     1020

cgctaccccg aggatgttta ccaaggaggc aatccatggt atctcgccac cctaggcgca     1080

tctgaattgc tttacgacgc cttgtaccag tgggacagac ttggcaaact tgaagtctcg     1140

gagacctcgt tgtcattctt caaagacttt gacgcgaccg tgaaaattgg ctcgtactcg     1200

aggaacagca agacctacaa gaaattgacc cagtccatca agtcgtacgc ggacgggttc     1260

atccagttag tgcagcagta cactccttct aatggatctc tggccgagca atacgatcgc     1320

aatacggctg ctcctctctc tgcaaacgat ctgacttggt catttgcctc tttcttgacg     1380

gctacgcaac gccgcgatgc cgtggttcct ccctcctggg gcgcaaagtc ggcaaacaaa     1440

gtcccaacca cttgttcagc ctcccctgtt gtgggtactt ataaggcgcc cacggcaact     1500

ttctcatcca agactaagtg cgtccccgct aaagatattg tgcctatcac gttctacctg     1560

attgagaaca cttactatgg agagaacgtc ttcatgagtg gcaacattac tgcgctgggt     1620

aactgggacg ccaagaaagg cttcccactc accgcaaacc tctacacgca agatcaaaac     1680

ttgtggttcg ccagtgtcga gttcatccca gcaggcacac cctttgagta caagtactac     1740

aaggtcgagc ccaatggcga tattacttgg gagaagggtc ccaaccgggt gttcgtcgct     1800

cccacgggat gcccagttca gcctcactcc aacgacgtgt ggcagttttg a              1851


<210>  2
<211>  616
<212>  PRT
<213>  Penicillium oxalicum

<400>  2

Met Arg Leu Thr Leu Leu Ser Gly Val Ala Gly Val Leu Cys Ala Gly 
1               5                   10                  15      


Gln Leu Thr Ala Ala Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro 
            20                  25                  30          


Phe Ile His Lys Glu Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn 
        35                  40                  45              


Leu Gly Gly Arg Gly Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe 
    50                  55                  60                  


Ile Ala Ser Pro Asn Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr 
65                  70                  75                  80  


Arg Asp Ser Ala Leu Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp 
                85                  90                  95      


Ser Arg Ala Lys Phe Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile 
            100                 105                 110         


Arg Asp Tyr Val Ser Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro 
        115                 120                 125             


Ser Gly Thr Leu Lys Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu 
    130                 135                 140                 


Ile Asp Leu Asn Pro Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp 
145                 150                 155                 160 


Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu 
                165                 170                 175     


Ile Ser His Gly Gln Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile 
            180                 185                 190         


Ile Ala Asn Asp Leu Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly 
        195                 200                 205             


Phe Asp Leu Trp Glu Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala 
    210                 215                 220                 


Val Gln His Arg Ala Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu 
225                 230                 235                 240 


Gly Lys Ser Cys Asp Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys 
                245                 250                 255     


Phe Leu Gln Ser Phe Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn 
            260                 265                 270         


Thr Gln Ala Ser Arg Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser 
        275                 280                 285             


Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln 
    290                 295                 300                 


Pro Cys Ser Ala Arg Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser 
305                 310                 315                 320 


Phe Arg Ser Ile Tyr Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala 
                325                 330                 335     


Ala Asn Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro 
            340                 345                 350         


Trp Tyr Leu Ala Thr Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu 
        355                 360                 365             


Tyr Gln Trp Asp Arg Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu 
    370                 375                 380                 


Ser Phe Phe Lys Asp Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser 
385                 390                 395                 400 


Arg Asn Ser Lys Thr Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr 
                405                 410                 415     


Ala Asp Gly Phe Ile Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly 
            420                 425                 430         


Ser Leu Ala Glu Gln Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala 
        435                 440                 445             


Asn Asp Leu Thr Trp Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg 
    450                 455                 460                 


Arg Asp Ala Val Val Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys 
465                 470                 475                 480 


Val Pro Thr Thr Cys Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala 
                485                 490                 495     


Pro Thr Ala Thr Phe Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp 
            500                 505                 510         


Ile Val Pro Ile Thr Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu 
        515                 520                 525             


Asn Val Phe Met Ser Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala 
    530                 535                 540                 


Lys Lys Gly Phe Pro Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn 
545                 550                 555                 560 


Leu Trp Phe Ala Ser Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu 
                565                 570                 575     


Tyr Lys Tyr Tyr Lys Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys 
            580                 585                 590         


Gly Pro Asn Arg Val Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro 
        595                 600                 605             


His Ser Asn Asp Val Trp Gln Phe 
    610                 615     


<210>  3
<211>  595
<212>  PRT
<213>  Penicillium oxalicum

<400>  3

Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro Phe Ile His Lys Glu 
1               5                   10                  15      


Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn Leu Gly Gly Arg Gly 
            20                  25                  30          


Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe Ile Ala Ser Pro Asn 
        35                  40                  45              


Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr Arg Asp Ser Ala Leu 
    50                  55                  60                  


Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp Ser Arg Ala Val Phe 
65                  70                  75                  80  


Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile Arg Asp Tyr Val Ser 
                85                  90                  95      


Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro Ser Gly Thr Leu Lys 
            100                 105                 110         


Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu Ile Asp Leu Asn Pro 
        115                 120                 125             


Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg 
    130                 135                 140                 


Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu Ile Ser His Gly Gln 
145                 150                 155                 160 


Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile Ile Ala Asn Asp Leu 
                165                 170                 175     


Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly Phe Asp Leu Trp Glu 
            180                 185                 190         


Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala 
        195                 200                 205             


Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu Gly Lys Ser Cys Asp 
    210                 215                 220                 


Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys Phe Leu Gln Ser Phe 
225                 230                 235                 240 


Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn Thr Gln Ala Ser Arg 
                245                 250                 255     


Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser Ile His Thr Phe Asp 
            260                 265                 270         


Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln Pro Cys Ser Ala Arg 
        275                 280                 285             


Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser Phe Arg Ser Ile Tyr 
    290                 295                 300                 


Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala Ala Asn Val Gly Arg 
305                 310                 315                 320 


Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr Leu Ala Thr 
                325                 330                 335     


Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu Tyr Gln Trp Asp Arg 
            340                 345                 350         


Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu Ser Phe Phe Lys Asp 
        355                 360                 365             


Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser Arg Asn Ser Lys Thr 
    370                 375                 380                 


Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr Ala Asp Gly Phe Ile 
385                 390                 395                 400 


Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly Ser Leu Ala Glu Gln 
                405                 410                 415     


Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala Asn Asp Leu Thr Trp 
            420                 425                 430         


Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg Arg Asp Ala Val Val 
        435                 440                 445             


Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys Val Pro Thr Thr Cys 
    450                 455                 460                 


Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala Pro Thr Ala Thr Phe 
465                 470                 475                 480 


Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp Ile Val Pro Ile Thr 
                485                 490                 495     


Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu Asn Val Phe Met Ser 
            500                 505                 510         


Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala Lys Lys Gly Phe Pro 
        515                 520                 525             


Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn Leu Trp Phe Ala Ser 
    530                 535                 540                 


Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu Tyr Lys Tyr Tyr Lys 
545                 550                 555                 560 


Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys Gly Pro Asn Arg Val 
                565                 570                 575     


Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro His Ser Asn Asp Val 
            580                 585                 590         


Trp Gln Phe 
        595 


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  4
atgcgtctca ctctattatc aggtg                                             25


<210>  5
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  5
acacaactgg ggatccacca tgcgtctcac tctattatc                              39


<210>  6
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  6
agatctcgag aagcttaaaa ctgccacacg tcgttgg                                37


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  7
gcagtctttc caattgac                                                     18


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  8
aattggaaag actgcccg                                                     18


<210>  9
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  9
acacaactgg ggatccacca tgcgtctcac tctattatc                              39


<210>  10
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  10
agatctcgag aagcttaaaa ctgccacacg tcgttgg                                37


