
                                SEQUENCE LISTING

<110> Glaxo Group Limited

<120> MODIFIED HGF-1K1 POLYPEPTIDE
  

<130> PB64756

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 183
<212> PRT
<213> Artificial Sequence

<220> 
<223> mutated version of Human Growth Factor NK1 domain

<400> 1
Tyr Ala Glu Gly Gln Arg Lys Arg Arg Asn Thr Ile His Glu Phe Lys
 1               5                  10                  15      
Lys Ser Ala Lys Thr Thr Leu Ile Lys Ile Asp Pro Ala Leu Lys Ile
            20                  25                  30          
Lys Thr Lys Lys Val Asn Thr Ala Asp Gln Cys Ala Asn Arg Cys Thr
        35                  40                  45              
Arg Asn Lys Gly Leu Pro Phe Thr Cys Lys Ala Phe Val Phe Asp Lys
    50                  55                  60                  
Ala Arg Lys Gln Cys Leu Trp Phe Pro Phe Asn Ser Met Ser Ser Gly
65                  70                  75                  80  
Val Lys Lys Glu Phe Gly His Glu Phe Asp Leu Tyr Glu Asn Lys Asp
                85                  90                  95      
Tyr Ile Arg Asn Cys Ile Ile Gly Glu Gly Glu Ser Tyr Lys Gly Thr
            100                 105                 110         
Val Ser Ile Thr Lys Ser Gly Ile Lys Cys Gln Pro Trp Ser Ser Met
        115                 120                 125             
Ile Pro His Glu His Ser Phe Leu Pro Ser Ser Tyr Arg Gly Lys Asp
    130                 135                 140                 
Leu Gln Glu Asn Tyr Cys Arg Asn Pro Arg Gly Glu Glu Gly Gly Pro
145                 150                 155                 160 
Trp Cys Phe Thr Ser Asn Pro Glu Val Arg Tyr Glu Val Cys Asp Ile
                165                 170                 175     
Pro Gln Cys Ser Glu Val Glu
            180             


<210> 2
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mammalian codon-optimised

<400> 2
tacgccgagg gccagcggaa gcggcggaac accatccacg agttcaagaa gtccgccaag 60
accaccctga tcaagatcga ccccgccctg aagatcaaga ccaagaaagt gaacaccgcc 120
gaccagtgcg ccaaccggtg caccagaaac aagggcctgc cctttacctg caaggccttc 180
gtgttcgaca aggcccggaa gcagtgcctg tggttcccct tcaactccat gtccagcggc 240
gtgaagaaag agttcggcca cgagttcgac ctgtacgaga acaaggacta catccggaac 300
tgcatcatcg gcgagggcga gagctacaag ggcaccgtgt ccatcaccaa gagcggcatc 360
aagtgccagc cctggtccag catgatcccc cacgagcaca gctttctgcc cagcagctac 420
cggggcaagg acctgcagga aaactactgc cggaacccca gaggcgaaga gggcggaccc 480
tggtgcttca ccagcaaccc cgaagtgcgc tacgaagtgt gcgacatccc ccagtgcagc 540
gaggtggaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593

<210> 3
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pichia codon-optimised

<400> 3
tatgctgaag gtcagagaaa gagaagaaac actatccacg agttcaagaa gtccgctaag 60
actactttga tcaagatcga cccagctttg aagatcaaga ctaagaaggt taacactgct 120
gaccagtgtg ctaacagatg tactagaaac aagggtttgc cattcacttg taaggctttc 180
gttttcgaca aggctagaaa gcagtgtttg tggttccctt tcaactccat gtcctccggt 240
gttaagaaag agttcggtca cgagttcgac ttgtacgaga acaaggacta catcagaaac 300
tgtatcatcg gtgagggtga gtcctacaag ggtactgttt ccatcactaa gtccggtatc 360
aagtgtcaac catggtcctc catgatccca cacgagcatt ctttcttgcc atcctcttac 420
agaggtaagg acttgcaaga gaactactgt agaaacccaa gaggtgaaga gggtggtcca 480
tggtgtttca cttctaaccc agaggttaga tacgaggttt gtgacatccc acagtgttct 540
gaggttgaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593

<210> 4
<211> 183
<212> PRT
<213> Artificial Sequence

<220> 
<223> mutated version of Human Growth Factor NK1 domain

<400> 4
Glu Ala Glu Ala Gln Arg Lys Arg Arg Asn Thr Ile His Glu Phe Lys
 1               5                  10                  15      
Lys Ser Ala Lys Thr Thr Leu Ile Lys Ile Asp Pro Ala Leu Lys Ile
            20                  25                  30          
Lys Thr Lys Lys Val Asn Thr Ala Asp Gln Cys Ala Asn Arg Cys Thr
        35                  40                  45              
Arg Asn Lys Gly Leu Pro Phe Thr Cys Lys Ala Phe Val Phe Asp Lys
    50                  55                  60                  
Ala Arg Lys Gln Cys Leu Trp Phe Pro Phe Asn Ser Met Ser Ser Gly
65                  70                  75                  80  
Val Lys Lys Glu Phe Gly His Glu Phe Asp Leu Tyr Glu Asn Lys Asp
                85                  90                  95      
Tyr Ile Arg Asn Cys Ile Ile Gly Glu Gly Glu Ser Tyr Lys Gly Thr
            100                 105                 110         
Val Ser Ile Thr Lys Ser Gly Ile Lys Cys Gln Pro Trp Ser Ser Met
        115                 120                 125             
Ile Pro His Glu His Ser Phe Leu Pro Ser Ser Tyr Arg Gly Lys Asp
    130                 135                 140                 
Leu Gln Glu Asn Tyr Cys Arg Asn Pro Arg Gly Glu Glu Gly Gly Pro
145                 150                 155                 160 
Trp Cys Phe Thr Ser Asn Pro Glu Val Arg Tyr Glu Val Cys Asp Ile
                165                 170                 175     
Pro Gln Cys Ser Glu Val Glu
            180             


<210> 5
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mammilian codon-optimised

<400> 5
gaggctgaag cacagcggaa gcggcggaac accatccacg agttcaagaa gtccgccaag 60
accaccctga tcaagatcga ccccgccctg aagatcaaga ccaagaaagt gaacaccgcc 120
gaccagtgcg ccaaccggtg caccagaaac aagggcctgc cctttacctg caaggccttc 180
gtgttcgaca aggcccggaa gcagtgcctg tggttcccct tcaactccat gtccagcggc 240
gtgaagaaag agttcggcca cgagttcgac ctgtacgaga acaaggacta catccggaac 300
tgcatcatcg gcgagggcga gagctacaag ggcaccgtgt ccatcaccaa gagcggcatc 360
aagtgccagc cctggtccag catgatcccc cacgagcaca gctttctgcc cagcagctac 420
cggggcaagg acctgcagga aaactactgc cggaacccca gaggcgaaga gggcggaccc 480
tggtgcttca ccagcaaccc cgaagtgcgc tacgaagtgt gcgacatccc ccagtgcagc 540
gaggtggaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593

<210> 6
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mammalian codon-optimised

<400> 6
gaggctgaag cacagagaaa gagaagaaac actatccacg agttcaagaa gtccgccaag 60
accaccctga tcaagatcga ccccgccctg aagatcaaga ccaagaaagt gaacaccgcc 120
gaccagtgcg ccaaccggtg caccagaaac aagggcctgc cctttacctg caaggccttc 180
gtgttcgaca aggcccggaa gcagtgcctg tggttcccct tcaactccat gtccagcggc 240
gtgaagaaag agttcggcca cgagttcgac ctgtacgaga acaaggacta catccggaac 300
tgcatcatcg gcgagggcga gagctacaag ggcaccgtgt ccatcaccaa gagcggcatc 360
aagtgccagc cctggtccag catgatcccc cacgagcaca gctttctgcc cagcagctac 420
cggggcaagg acctgcagga aaactactgc cggaacccca gaggcgaaga gggcggaccc 480
tggtgcttca ccagcaaccc cgaagtgcgc tacgaagtgt gcgacatccc ccagtgcagc 540
gaggtggaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593

<210> 7
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pichia codon-optimised

<400> 7
gaggctgaag cacagagaaa gagaagaaac actatccacg agttcaagaa gtccgctaag 60
actactttga tcaagatcga cccagctttg aagatcaaga ctaagaaggt taacactgct 120
gaccagtgtg ctaacagatg tactagaaac aagggtttgc cattcacttg taaggctttc 180
gttttcgaca aggctagaaa gcagtgtttg tggttccctt tcaactccat gtcctccggt 240
gttaagaaag agttcggtca cgagttcgac ttgtacgaga acaaggacta catcagaaac 300
tgtatcatcg gtgagggtga gtcctacaag ggtactgttt ccatcactaa gtccggtatc 360
aagtgtcaac catggtcctc catgatccca cacgagcatt ctttcttgcc atcctcttac 420
agaggtaagg acttgcaaga gaactactgt agaaacccaa gaggtgaaga gggtggtcca 480
tggtgtttca cttctaaccc agaggttaga tacgaggttt gtgacatccc acagtgttct 540
gaggttgaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593

<210> 8
<211> 183
<212> PRT
<213> Artificial Sequence

<220> 
<223> mutated version of Human Growth Factor NK1 domain

<400> 8
Tyr Val Glu Gly Gln Arg Lys Arg Arg Asn Thr Ile His Glu Phe Lys
 1               5                  10                  15      
Lys Ser Ala Lys Thr Thr Leu Ile Lys Ile Asp Pro Ala Leu Lys Ile
            20                  25                  30          
Lys Thr Lys Lys Val Asn Thr Ala Asp Gln Cys Ala Asn Arg Cys Thr
        35                  40                  45              
Arg Asn Lys Gly Leu Pro Phe Thr Cys Lys Ala Phe Val Phe Asp Lys
    50                  55                  60                  
Ala Arg Lys Gln Cys Leu Trp Phe Pro Phe Asn Ser Met Ser Ser Gly
65                  70                  75                  80  
Val Lys Lys Glu Phe Gly His Glu Phe Asp Leu Tyr Glu Asn Lys Asp
                85                  90                  95      
Tyr Ile Arg Asn Cys Ile Ile Gly Glu Gly Glu Ser Tyr Lys Gly Thr
            100                 105                 110         
Val Ser Ile Thr Lys Ser Gly Ile Lys Cys Gln Pro Trp Ser Ser Met
        115                 120                 125             
Ile Pro His Glu His Ser Phe Leu Pro Ser Ser Tyr Arg Gly Lys Asp
    130                 135                 140                 
Leu Gln Glu Asn Tyr Cys Arg Asn Pro Arg Gly Glu Glu Gly Gly Pro
145                 150                 155                 160 
Trp Cys Phe Thr Ser Asn Pro Glu Val Arg Tyr Glu Val Cys Asp Ile
                165                 170                 175     
Pro Gln Cys Ser Glu Val Glu
            180             


<210> 9
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Mammalian codon-optimised

<400> 9
tacgtcgagg gccagcggaa gcggcggaac accatccacg agttcaagaa gtccgccaag 60
accaccctga tcaagatcga ccccgccctg aagatcaaga ccaagaaagt gaacaccgcc 120
gaccagtgcg ccaaccggtg caccagaaac aagggcctgc cctttacctg caaggccttc 180
gtgttcgaca aggcccggaa gcagtgcctg tggttcccct tcaactccat gtccagcggc 240
gtgaagaaag agttcggcca cgagttcgac ctgtacgaga acaaggacta catccggaac 300
tgcatcatcg gcgagggcga gagctacaag ggcaccgtgt ccatcaccaa gagcggcatc 360
aagtgccagc cctggtccag catgatcccc cacgagcaca gctttctgcc cagcagctac 420
cggggcaagg acctgcagga aaactactgc cggaacccca gaggcgaaga gggcggaccc 480
tggtgcttca ccagcaaccc cgaagtgcgc tacgaagtgt gcgacatccc ccagtgcagc 540
gaggtggaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593

<210> 10
<211> 593
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pichia codon-optimised

<400> 10
tatgttgaag gtcagagaaa gagaagaaac actatccacg agttcaagaa gtccgctaag 60
actactttga tcaagatcga cccagctttg aagatcaaga ctaagaaggt taacactgct 120
gaccagtgtg ctaacagatg tactagaaac aagggtttgc cattcacttg taaggctttc 180
gttttcgaca aggctagaaa gcagtgtttg tggttccctt tcaactccat gtcctccggt 240
gttaagaaag agttcggtca cgagttcgac ttgtacgaga acaaggacta catcagaaac 300
tgtatcatcg gtgagggtga gtcctacaag ggtactgttt ccatcactaa gtccggtatc 360
aagtgtcaac catggtcctc catgatccca cacgagcatt ctttcttgcc atcctcttac 420
agaggtaagg acttgcaaga gaactactgt agaaacccaa gaggtgaaga gggtggtcca 480
tggtgtttca cttctaaccc agaggttaga tacgaggttt gtgacatccc acagtgttct 540
gaggttgaaa agcttgagct catggcgcgc ctaggccttg acggccttcc gcc        593
