                         SEQUENCE LISTING

<110>  Health Protection Agency
 
<120>  Thermostable assay reagents

<130>  P36821WO-PJG

<150>  GB1115911.8
<151>  2011-09-14

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  408
<212>  PRT
<213>  Methanosarcina thermophila

<400>  1

Met Lys Val Leu Val Ile Asn Ala Gly Ser Ser Ser Leu Lys Tyr Gln 
1               5                   10                  15      


Leu Ile Asp Met Thr Asn Glu Ser Ala Leu Ala Val Gly Leu Cys Glu 
            20                  25                  30          


Arg Ile Gly Ile Asp Asn Ser Ile Ile Thr Gln Lys Lys Phe Asp Gly 
        35                  40                  45              


Lys Lys Leu Glu Lys Leu Thr Asp Leu Pro Thr His Lys Asp Ala Leu 
    50                  55                  60                  


Glu Glu Val Val Lys Ala Leu Thr Asp Asp Glu Phe Gly Val Ile Lys 
65                  70                  75                  80  


Asp Met Gly Glu Ile Asn Ala Val Gly His Arg Val Val His Gly Gly 
                85                  90                  95      


Glu Lys Phe Thr Thr Ser Ala Leu Tyr Asp Glu Gly Val Glu Lys Ala 
            100                 105                 110         


Ile Lys Asp Cys Phe Glu Leu Ala Pro Leu His Asn Pro Pro Asn Met 
        115                 120                 125             


Met Gly Ile Ser Ala Cys Ala Glu Ile Met Pro Gly Thr Pro Met Val 
    130                 135                 140                 


Ile Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Pro Tyr Ala Tyr 
145                 150                 155                 160 


Met Tyr Ala Leu Pro Tyr Asp Leu Tyr Glu Lys His Gly Val Arg Lys 
                165                 170                 175     


Tyr Gly Phe His Gly Thr Ser His Lys Tyr Val Ala Glu Arg Ala Ala 
            180                 185                 190         


Leu Met Leu Gly Lys Pro Ala Glu Glu Thr Lys Ile Ile Thr Cys His 
        195                 200                 205             


Leu Gly Asn Gly Ser Ser Ile Thr Ala Val Glu Gly Gly Lys Ser Val 
    210                 215                 220                 


Glu Thr Ser Met Gly Phe Thr Pro Leu Glu Gly Leu Ala Met Gly Thr 
225                 230                 235                 240 


Arg Cys Gly Ser Ile Asp Pro Ala Ile Val Pro Phe Leu Met Glu Lys 
                245                 250                 255     


Glu Gly Leu Thr Thr Arg Glu Ile Asp Thr Leu Met Asn Lys Lys Ser 
            260                 265                 270         


Gly Val Leu Gly Val Ser Gly Leu Ser Asn Asp Phe Arg Asp Leu Asp 
        275                 280                 285             


Glu Ala Ala Ser Lys Gly Asn Arg Lys Ala Glu Leu Ala Leu Glu Ile 
    290                 295                 300                 


Phe Ala Tyr Lys Val Lys Lys Phe Ile Gly Glu Tyr Ser Ala Val Leu 
305                 310                 315                 320 


Asn Gly Ala Asp Ala Val Val Phe Thr Ala Gly Ile Gly Glu Asn Ser 
                325                 330                 335     


Ala Ser Ile Arg Lys Arg Ile Leu Thr Gly Leu Asp Gly Ile Gly Ile 
            340                 345                 350         


Lys Ile Asp Asp Glu Lys Asn Lys Ile Arg Gly Gln Glu Ile Asp Ile 
        355                 360                 365             


Ser Thr Pro Asp Ala Lys Val Arg Val Phe Val Ile Pro Thr Asn Glu 
    370                 375                 380                 


Glu Leu Ala Ile Ala Arg Glu Thr Lys Glu Ile Val Glu Thr Glu Val 
385                 390                 395                 400 


Lys Leu Arg Ser Ser Ile Pro Val 
                405             


<210>  2
<211>  587
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  2

Met Lys Arg Lys Thr Lys Ile Val Cys Thr Ile Gly Pro Ala Ser Glu 
1               5                   10                  15      


Ser Val Asp Lys Leu Val Gln Leu Met Glu Ala Gly Met Asn Val Ala 
            20                  25                  30          


Arg Leu Asn Phe Ser His Gly Asp His Glu Glu His Gly Arg Arg Ile 
        35                  40                  45              


Ala Asn Ile Arg Glu Ala Ala Lys Arg Thr Gly Arg Thr Val Ala Ile 
    50                  55                  60                  


Leu Leu Asp Thr Lys Gly Pro Glu Ile Arg Thr His Asn Met Glu Asn 
65                  70                  75                  80  


Gly Ala Ile Glu Leu Lys Glu Gly Ser Lys Leu Val Ile Ser Met Ser 
                85                  90                  95      


Glu Val Leu Gly Thr Pro Glu Lys Ile Ser Val Thr Tyr Pro Ser Leu 
            100                 105                 110         


Ile Asp Asp Val Ser Val Gly Ala Lys Ile Leu Leu Asp Asp Gly Leu 
        115                 120                 125             


Ile Ser Leu Glu Val Asn Ala Val Asp Lys Gln Ala Gly Glu Ile Val 
    130                 135                 140                 


Thr Thr Val Leu Asn Gly Gly Val Leu Lys Asn Lys Lys Gly Val Asn 
145                 150                 155                 160 


Val Pro Gly Val Lys Val Asn Leu Pro Gly Ile Thr Glu Lys Asp Arg 
                165                 170                 175     


Ala Asp Ile Leu Phe Gly Ile Arg Gln Gly Ile Asp Phe Ile Ala Ala 
            180                 185                 190         


Ser Phe Val Arg Arg Ala Ser Asp Val Leu Glu Ile Arg Glu Leu Leu 
        195                 200                 205             


Glu Ala His Asp Ala Leu His Ile Gln Ile Ile Ala Lys Ile Glu Asn 
    210                 215                 220                 


Glu Glu Gly Val Ala Asn Ile Asp Glu Ile Leu Glu Ala Ala Asp Gly 
225                 230                 235                 240 


Leu Met Val Ala Arg Gly Asp Leu Gly Val Glu Ile Pro Ala Glu Glu 
                245                 250                 255     


Val Pro Leu Ile Gln Lys Leu Leu Ile Lys Lys Cys Asn Met Leu Gly 
            260                 265                 270         


Lys Pro Val Ile Thr Ala Thr Gln Met Leu Asp Ser Met Gln Arg Asn 
        275                 280                 285             


Pro Arg Pro Thr Arg Ala Glu Ala Ser Asp Val Ala Asn Ala Ile Phe 
    290                 295                 300                 


Asp Gly Thr Asp Ala Val Met Leu Ser Gly Glu Thr Ala Ala Gly Gln 
305                 310                 315                 320 


Tyr Pro Val Glu Ala Val Lys Thr Met His Gln Ile Ala Leu Arg Thr 
                325                 330                 335     


Glu Gln Ala Leu Glu His Arg Asp Ile Leu Ser Gln Arg Thr Lys Glu 
            340                 345                 350         


Ser Gln Thr Thr Ile Thr Asp Ala Ile Gly Gln Ser Val Ala His Thr 
        355                 360                 365             


Ala Leu Asn Leu Asp Val Ala Ala Ile Val Thr Pro Thr Val Ser Gly 
    370                 375                 380                 


Lys Thr Pro Gln Met Val Ala Lys Tyr Arg Pro Lys Ala Pro Ile Ile 
385                 390                 395                 400 


Ala Val Thr Ser Asn Glu Ala Val Ser Arg Arg Leu Ala Leu Val Trp 
                405                 410                 415     


Gly Val Tyr Thr Lys Glu Ala Pro His Val Asn Thr Thr Asp Glu Met 
            420                 425                 430         


Leu Asp Val Ala Val Asp Ala Ala Val Arg Ser Gly Leu Val Lys His 
        435                 440                 445             


Gly Asp Leu Val Val Ile Thr Ala Gly Val Pro Val Gly Glu Thr Gly 
    450                 455                 460                 


Ser Thr Asn Leu Met Lys Val His Val Ile Ser Asp Leu Leu Ala Lys 
465                 470                 475                 480 


Gly Gln Gly Ile Gly Arg Lys Ser Ala Phe Gly Lys Ala Val Val Ala 
                485                 490                 495     


Lys Thr Ala Glu Glu Ala Arg Gln Lys Met Val Asp Gly Gly Ile Leu 
            500                 505                 510         


Val Thr Val Ser Thr Asp Ala Asp Met Met Pro Ala Ile Glu Lys Ala 
        515                 520                 525             


Ala Ala Ile Ile Thr Glu Glu Gly Gly Leu Thr Ser His Ala Ala Val 
    530                 535                 540                 


Val Gly Leu Ser Leu Gly Ile Pro Val Ile Val Gly Val Glu Asn Ala 
545                 550                 555                 560 


Thr Thr Leu Phe Lys Asp Gly Gln Glu Ile Thr Val Asp Gly Gly Phe 
                565                 570                 575     


Gly Ala Val Tyr Arg Gly His Ala Ser Val Leu 
            580                 585         


<210>  3
<211>  244
<212>  PRT
<213>  Pyrococcus furiosus

<400>  3

Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro Arg 
1               5                   10                  15      


Gly Ser His Met Arg Ile Val Phe Asp Ile Gly Gly Ser Val Leu Val 
            20                  25                  30          


Pro Glu Asn Pro Asp Ile Asp Phe Ile Lys Glu Ile Ala Tyr Gln Leu 
        35                  40                  45              


Thr Lys Val Ser Glu Asp His Glu Val Ala Val Val Val Gly Gly Gly 
    50                  55                  60                  


Lys Leu Ala Arg Lys Tyr Ile Glu Val Ala Glu Lys Phe Asn Ser Ser 
65                  70                  75                  80  


Glu Thr Phe Lys Asp Phe Ile Gly Ile Gln Ile Thr Arg Ala Asn Ala 
                85                  90                  95      


Met Leu Leu Ile Ala Ala Leu Arg Glu Lys Ala Tyr Pro Val Val Val 
            100                 105                 110         


Glu Asp Phe Trp Glu Ala Trp Lys Ala Val Gln Leu Lys Lys Ile Pro 
        115                 120                 125             


Val Met Gly Gly Thr His Pro Gly His Thr Thr Asp Ala Val Ala Ala 
    130                 135                 140                 


Leu Leu Ala Glu Phe Leu Lys Ala Asp Leu Leu Val Val Ile Thr Asn 
145                 150                 155                 160 


Val Asp Gly Val Tyr Thr Ala Asp Pro Lys Lys Asp Pro Thr Ala Lys 
                165                 170                 175     


Lys Ile Lys Lys Met Lys Pro Glu Glu Leu Leu Glu Ile Val Gly Lys 
            180                 185                 190         


Gly Ile Glu Lys Ala Gly Ser Ser Ser Val Ile Asp Pro Leu Ala Ala 
        195                 200                 205             


Lys Ile Ile Ala Arg Ser Gly Ile Lys Thr Ile Val Ile Gly Lys Glu 
    210                 215                 220                 


Asp Ala Lys Asp Leu Phe Arg Val Ile Lys Gly Asp His Asn Gly Thr 
225                 230                 235                 240 


Thr Ile Glu Pro 
                


<210>  4
<211>  375
<212>  PRT
<213>  Thermotoga maritima

<400>  4

Met Phe Arg Ile Leu Thr Ile Asn Pro Gly Ser Thr Ser Thr Lys Leu 
1               5                   10                  15      


Ser Ile Phe Glu Asp Glu Arg Met Val Lys Met Gln Asn Phe Ser His 
            20                  25                  30          


Ser Pro Asp Glu Leu Gly Arg Phe Gln Lys Ile Leu Asp Gln Leu Glu 
        35                  40                  45              


Phe Arg Glu Lys Ile Ala Arg Gln Phe Val Glu Glu Thr Gly Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Phe Ser Ala Phe Val Ser Arg Gly Gly Leu Leu Asp Pro 
65                  70                  75                  80  


Ile Pro Gly Gly Val Tyr Leu Val Asp Gly Leu Met Ile Lys Thr Leu 
                85                  90                  95      


Lys Ser Gly Lys Asn Gly Glu His Ala Ser Asn Leu Gly Ala Ile Ile 
            100                 105                 110         


Ala His Arg Phe Ser Ser Glu Thr Gly Val Pro Ala Tyr Val Val Asp 
        115                 120                 125             


Pro Val Val Val Asp Glu Met Glu Asp Val Ala Arg Val Ser Gly His 
    130                 135                 140                 


Pro Asn Tyr Gln Arg Lys Ser Ile Phe His Ala Leu Asn Gln Lys Thr 
145                 150                 155                 160 


Val Ala Lys Glu Val Ala Arg Met Met Asn Lys Arg Tyr Glu Glu Met 
                165                 170                 175     


Asn Leu Val Val Ala His Met Gly Gly Gly Ile Ser Ile Ala Ala His 
            180                 185                 190         


Arg Lys Gly Arg Val Ile Asp Val Asn Asn Ala Leu Asp Gly Asp Gly 
        195                 200                 205             


Pro Phe Thr Pro Glu Arg Ser Gly Thr Leu Pro Leu Thr Gln Leu Val 
    210                 215                 220                 


Asp Leu Cys Phe Ser Gly Lys Phe Thr Tyr Glu Glu Met Lys Lys Arg 
225                 230                 235                 240 


Ile Val Gly Asn Gly Gly Leu Val Ala Tyr Leu Gly Thr Ser Asp Ala 
                245                 250                 255     


Arg Glu Val Val Arg Arg Ile Lys Gln Gly Asp Glu Trp Ala Lys Arg 
            260                 265                 270         


Val Tyr Arg Ala Met Ala Tyr Gln Ile Ala Lys Trp Ile Gly Lys Met 
        275                 280                 285             


Ala Ala Val Leu Lys Gly Glu Val Asp Phe Ile Val Leu Thr Gly Gly 
    290                 295                 300                 


Leu Ala His Glu Lys Glu Phe Leu Val Pro Trp Ile Thr Lys Arg Val 
305                 310                 315                 320 


Ser Phe Ile Ala Pro Val Leu Val Phe Pro Gly Ser Asn Glu Glu Lys 
                325                 330                 335     


Ala Leu Ala Leu Ser Ala Leu Arg Val Leu Arg Gly Glu Glu Lys Pro 
            340                 345                 350         


Lys Asn Tyr Ser Glu Glu Ser Arg Arg Trp Arg Glu Arg Tyr Asp Ser 
        355                 360                 365             


Tyr Leu Asp Gly Ile Leu Arg 
    370                 375 


<210>  5
<211>  220
<212>  PRT
<213>  Thermotoga maritima

<400>  5

Met Met Ala Tyr Leu Val Phe Leu Gly Pro Pro Gly Ala Gly Lys Gly 
1               5                   10                  15      


Thr Tyr Ala Lys Arg Leu Gln Glu Ile Thr Gly Ile Pro His Ile Ser 
            20                  25                  30          


Thr Gly Asp Ile Phe Arg Asp Ile Val Lys Lys Glu Asn Asp Glu Leu 
        35                  40                  45              


Gly Lys Lys Ile Lys Glu Ile Met Glu Arg Gly Glu Leu Val Pro Asp 
    50                  55                  60                  


Glu Leu Val Asn Glu Val Val Lys Arg Arg Leu Ser Glu Lys Asp Cys 
65                  70                  75                  80  


Glu Arg Gly Phe Ile Leu Asp Gly Tyr Pro Arg Thr Val Ala Gln Ala 
                85                  90                  95      


Glu Phe Leu Asp Gly Phe Leu Lys Thr Gln Asn Lys Glu Leu Thr Ala 
            100                 105                 110         


Ala Val Leu Phe Glu Val Pro Glu Glu Val Val Val Gln Arg Leu Thr 
        115                 120                 125             


Ala Arg Arg Ile Cys Pro Lys Cys Gly Arg Ile Tyr Asn Leu Ile Ser 
    130                 135                 140                 


Leu Pro Pro Lys Glu Asp Glu Leu Cys Asp Asp Cys Lys Val Lys Leu 
145                 150                 155                 160 


Val Gln Arg Glu Asp Asp Lys Glu Glu Thr Val Arg His Arg Tyr Lys 
                165                 170                 175     


Val Tyr Leu Glu Lys Thr Gln Pro Val Ile Asp Tyr Tyr Asp Lys Lys 
            180                 185                 190         


Gly Ile Leu Lys Arg Val Asp Gly Thr Ile Gly Ile Asp Asn Val Ile 
        195                 200                 205             


Ala Glu Val Leu Lys Ile Ile Gly Trp Ser Asp Lys 
    210                 215                 220 


<210>  6
<211>  194
<212>  PRT
<213>  Sulfolobus acidocaldarius

<400>  6

Met Lys Ile Gly Ile Val Thr Gly Ile Pro Gly Val Gly Lys Ser Thr 
1               5                   10                  15      


Val Leu Ala Lys Val Lys Glu Ile Leu Asp Asn Gln Gly Ile Asn Asn 
            20                  25                  30          


Lys Ile Ile Asn Tyr Gly Asp Phe Met Leu Ala Thr Ala Leu Lys Leu 
        35                  40                  45              


Gly Tyr Ala Lys Asp Arg Asp Glu Met Arg Lys Leu Ser Val Glu Lys 
    50                  55                  60                  


Gln Lys Lys Leu Gln Ile Asp Ala Ala Lys Gly Ile Ala Glu Glu Ala 
65                  70                  75                  80  


Arg Ala Gly Gly Glu Gly Tyr Leu Phe Ile Asp Thr His Ala Val Ile 
                85                  90                  95      


Arg Thr Pro Ser Gly Tyr Leu Pro Gly Leu Pro Ser Tyr Val Ile Thr 
            100                 105                 110         


Glu Ile Asn Pro Ser Val Ile Phe Leu Leu Glu Ala Asp Pro Lys Ile 
        115                 120                 125             


Ile Leu Ser Arg Gln Lys Arg Asp Thr Thr Arg Asn Arg Asn Asp Tyr 
    130                 135                 140                 


Ser Asp Glu Ser Val Ile Leu Glu Thr Ile Asn Phe Ala Arg Tyr Ala 
145                 150                 155                 160 


Ala Thr Ala Ser Ala Val Leu Ala Gly Ser Thr Val Lys Val Ile Val 
                165                 170                 175     


Asn Val Glu Gly Asp Pro Ser Ile Ala Ala Asn Glu Ile Ile Arg Ser 
            180                 185                 190         


Met Lys 
        


<210>  7
<211>  981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Adenylate kinase from Sulfolobus acidocaldarius with unstructured
       linker and VNAR sequence

<400>  7
atgaagatcg gcattgtgac cggcattccg ggcgttggca aaagcaccgt tctggcaaag       60

gtgaaggaga tcctggacaa ccagggcatt aataacaaaa ttattaatta tggtgatttt      120

atgctggcga ccgcgctgaa gctgggctac gcaaaagatc gtgacgaaat gcgcaaactg      180

agcgtggaaa aacagaagaa gctgcagatt gatgcggcga agggcattgc ggaagaggca      240

cgcgcgggcg gcgaaggcta cctgtttatc gatacccatg cggtgatccg caccccgagc      300

ggttatctgc cgggcctgcc gtcttacgtg attacggaaa tcaacccgag cgttattttt      360

ctgctggagg cagatccgaa gattattctg agccgccaga agcgcgatac cacccgcaac      420

cgcaacgatt atagcgacga aagcgttatc ctggagacca tcaactttgc gcgctatgcg      480

gcaaccgcga gcgcggttct ggcaggctct accgttaaag tgatcgtgaa cgtggagggt      540

gatccaagca tcgcggcgaa cgaaatcatt cgcagcatga aacagtcgac cggtggtggt      600

ggttctggtg gtggtggttc tggtggtggt ggttctggat ccgcacgtgt tgatcagaca      660

ccgcgtagcg ttaccaaaga aaccggtgaa agcctgacca ttaattgtgt tctgcgtgat      720

gcaagctatg cactgggtag cacctgttgg tatcgtaaaa aaagcggcag caccaatgaa      780

gaaagcatta gcaaaggtgg tcgttatgtt gaaaccgtga atagcggtag caaaagcttt      840

agcctgcgta ttaatgatct gaccgttgaa gatggtggga cctaccgttg tggtctgggt      900

gttgccggtg gttattgtga ttatgcactg tgcagcagcc gttatgcaga atgtggtgat      960

ggtactgcag ttaccgtgaa t                                                981


<210>  8
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VNAR sequence for hen egg lysozyme specific single domain 
       antibody from shark

<400>  8

Gly Ser Ala Arg Val Asp Gln Thr Pro Arg Ser Val Thr Lys Glu Thr 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ala Ser Tyr Ala 
            20                  25                  30          


Leu Gly Ser Thr Cys Trp Tyr Arg Lys Lys Ser Gly Ser Thr Asn Glu 
        35                  40                  45              


Glu Ser Ile Ser Lys Gly Gly Arg Tyr Val Glu Thr Val Asn Ser Gly 
    50                  55                  60                  


Ser Lys Ser Phe Ser Leu Arg Ile Asn Asp Leu Thr Val Glu Asp Gly 
65                  70                  75                  80  


Gly Thr Tyr Arg Cys Gly Leu Gly Val Ala Gly Gly Tyr Cys Asp Tyr 
                85                  90                  95      


Ala Leu Cys Ser Ser Arg Tyr Ala Glu Cys Gly Asp Gly Thr Ala Val 
            100                 105                 110         


Thr Val Asn 
        115 


<210>  9
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for adenylate kinase from Sulfolobus 
       acidocaldarius with unstructured linker and VNAR sequence

<400>  9

Met Leu Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp Arg Asp Glu 
1               5                   10                  15      


Met Arg Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln Ile Asp Ala 
            20                  25                  30          


Ala Lys Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu Gly Tyr Leu 
        35                  40                  45              


Phe Ile Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly Tyr Leu Pro 
    50                  55                  60                  


Gly Leu Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser Val Ile Phe 
65                  70                  75                  80  


Leu Leu Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln Lys Arg Asp 
                85                  90                  95      


Thr Thr Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val Ile Leu Glu 
            100                 105                 110         


Thr Ile Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala Val Leu Ala 
        115                 120                 125             


Gly Ser Thr Val Lys Val Ile Val Asn Val Glu Gly Asp Pro Ser Ile 
    130                 135                 140                 


Ala Ala Asn Glu Ile Ile Arg Ser Met Lys Gln Ser Thr Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ser Ala Arg 
                165                 170                 175     


Val Asp Gln Thr Pro Arg Ser Val Thr Lys Glu Thr Gly Glu Ser Leu 
            180                 185                 190         


Thr Ile Asn Cys Val Leu Arg Asp Ala Ser Tyr Ala Leu Gly Ser Thr 
        195                 200                 205             


Cys Trp Tyr Arg Lys Lys Ser Gly Ser Thr Asn Glu Glu Ser Ile Ser 
    210                 215                 220                 


Lys Gly Gly Arg Tyr Val Glu Thr Val Asn Ser Gly Ser Lys Ser Phe 
225                 230                 235                 240 


Ser Leu Arg Ile Asn Asp Leu Thr Val Glu Asp Gly Gly Thr Tyr Arg 
                245                 250                 255     


Cys Gly Leu Gly Val Ala Gly Gly Tyr Cys Asp Tyr Ala Leu Cys Ser 
            260                 265                 270         


Ser Arg Tyr Ala Glu Cys Gly Asp Gly Thr Ala Val Thr Val Asn 
        275                 280                 285         


<210>  10
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for adenylate kinase from Sulfolobus 
       acidocaldarius with helical linker and VNAR sequence

<400>  10

Met Leu Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp Arg Asp Glu 
1               5                   10                  15      


Met Arg Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln Ile Asp Ala 
            20                  25                  30          


Ala Lys Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu Gly Tyr Leu 
        35                  40                  45              


Phe Ile Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly Tyr Leu Pro 
    50                  55                  60                  


Gly Leu Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser Val Ile Phe 
65                  70                  75                  80  


Leu Leu Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln Lys Arg Asp 
                85                  90                  95      


Thr Thr Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val Ile Leu Glu 
            100                 105                 110         


Thr Ile Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala Val Leu Ala 
        115                 120                 125             


Gly Ser Thr Val Lys Val Ile Val Asn Val Glu Gly Asp Pro Ser Ile 
    130                 135                 140                 


Ala Ala Asn Glu Ile Ile Arg Ser Met Lys Gln Ser Thr Leu Ala Glu 
145                 150                 155                 160 


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
                165                 170                 175     


Ala Ala Lys Ala Ala Ala Gly Ser Ala Arg Val Asp Gln Thr Pro Arg 
            180                 185                 190         


Ser Val Thr Lys Glu Thr Gly Glu Ser Leu Thr Ile Asn Cys Val Leu 
        195                 200                 205             


Arg Asp Ala Ser Tyr Ala Leu Gly Ser Thr Cys Trp Tyr Arg Lys Lys 
    210                 215                 220                 


Ser Gly Ser Thr Asn Glu Glu Ser Ile Ser Lys Gly Gly Arg Tyr Val 
225                 230                 235                 240 


Glu Thr Val Asn Ser Gly Ser Lys Ser Phe Ser Leu Arg Ile Asn Asp 
                245                 250                 255     


Leu Thr Val Glu Asp Gly Gly Thr Tyr Arg Cys Gly Leu Gly Val Ala 
            260                 265                 270         


Gly Gly Tyr Cys Asp Tyr Ala Leu Cys Ser Ser Arg Tyr Ala Glu Cys 
        275                 280                 285             


Gly Asp Gly Thr Ala Val Thr Val Asn 
    290                 295         


<210>  11
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for VNAR sequence with helical linker and
       adenylate kinase from Sulfolobus acidocaldarius

<400>  11

Met Gly Ser Ala Arg Val Asp Gln Thr Pro Arg Ser Val Thr Lys Glu 
1               5                   10                  15      


Thr Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ala Ser Tyr 
            20                  25                  30          


Ala Leu Gly Ser Thr Cys Trp Tyr Arg Lys Lys Ser Gly Ser Thr Asn 
        35                  40                  45              


Glu Glu Ser Ile Ser Lys Gly Gly Arg Tyr Val Glu Thr Val Asn Ser 
    50                  55                  60                  


Gly Ser Lys Ser Phe Ser Leu Arg Ile Asn Asp Leu Thr Val Glu Asp 
65                  70                  75                  80  


Gly Gly Thr Tyr Arg Cys Gly Leu Gly Val Ala Gly Gly Tyr Cys Asp 
                85                  90                  95      


Tyr Ala Leu Cys Ser Ser Arg Tyr Ala Glu Cys Gly Asp Gly Thr Ala 
            100                 105                 110         


Val Thr Val Asn Ser Thr Leu Ala Glu Ala Ala Ala Lys Glu Ala Ala 
        115                 120                 125             


Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ala Gly 
    130                 135                 140                 


Ser Met Leu Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp Arg Asp 
145                 150                 155                 160 


Glu Met Arg Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln Ile Asp 
                165                 170                 175     


Ala Ala Lys Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu Gly Tyr 
            180                 185                 190         


Leu Phe Ile Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly Tyr Leu 
        195                 200                 205             


Pro Gly Leu Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser Val Ile 
    210                 215                 220                 


Phe Leu Leu Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln Lys Arg 
225                 230                 235                 240 


Asp Thr Thr Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val Ile Leu 
                245                 250                 255     


Glu Thr Ile Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala Val Leu 
            260                 265                 270         


Ala Gly Ser Thr Val Lys Val Ile Val Asn Val Glu Gly Asp Pro Ser 
        275                 280                 285             


Ile Ala Ala Asn Glu Ile Ile Arg Ser Met Lys 
    290                 295                 


<210>  12
<211>  289
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for VNAR sequence with unstructured 
       linker and adenylate kinase from Sulfolobus acidocaldarius

<400>  12

Met Gly Ser Ala Arg Val Asp Gln Thr Pro Arg Ser Val Thr Lys Glu 
1               5                   10                  15      


Thr Gly Glu Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ala Ser Tyr 
            20                  25                  30          


Ala Leu Gly Ser Thr Cys Trp Tyr Arg Lys Lys Ser Gly Ser Thr Asn 
        35                  40                  45              


Glu Glu Ser Ile Ser Lys Gly Gly Arg Tyr Val Glu Thr Val Asn Ser 
    50                  55                  60                  


Gly Ser Lys Ser Phe Ser Leu Arg Ile Asn Asp Leu Thr Val Glu Asp 
65                  70                  75                  80  


Gly Gly Thr Tyr Arg Cys Gly Leu Gly Val Ala Gly Gly Tyr Cys Asp 
                85                  90                  95      


Tyr Ala Leu Cys Ser Ser Arg Tyr Ala Glu Cys Gly Asp Gly Thr Ala 
            100                 105                 110         


Val Thr Val Asn Ser Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Ser Met Leu Ala Thr Ala Leu Lys Leu Gly 
    130                 135                 140                 


Tyr Ala Lys Asp Arg Asp Glu Met Arg Lys Leu Ser Val Glu Lys Gln 
145                 150                 155                 160 


Lys Lys Leu Gln Ile Asp Ala Ala Lys Gly Ile Ala Glu Glu Ala Arg 
                165                 170                 175     


Ala Gly Gly Glu Gly Tyr Leu Phe Ile Asp Thr His Ala Val Ile Arg 
            180                 185                 190         


Thr Pro Ser Gly Tyr Leu Pro Gly Leu Pro Ser Tyr Val Ile Thr Glu 
        195                 200                 205             


Ile Asn Pro Ser Val Ile Phe Leu Leu Glu Ala Asp Pro Lys Ile Ile 
    210                 215                 220                 


Leu Ser Arg Gln Lys Arg Asp Thr Thr Arg Asn Arg Asn Asp Tyr Ser 
225                 230                 235                 240 


Asp Glu Ser Val Ile Leu Glu Thr Ile Asn Phe Ala Arg Tyr Ala Ala 
                245                 250                 255     


Thr Ala Ser Ala Val Leu Ala Gly Ser Thr Val Lys Val Ile Val Asn 
            260                 265                 270         


Val Glu Gly Asp Pro Ser Ile Ala Ala Asn Glu Ile Ile Arg Ser Met 
        275                 280                 285             


Lys 
    


<210>  13
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH sequence for hen egg lysozyme specific single domain antibody
       from camelid

<400>  13

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gln 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Ala Thr Ser Ser Ser Asn 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Val Ile Asp Thr Gly Arg Gly Asn Thr Ala Tyr Ala Asp Ser Val 
    50                  55                  60                  


Gln Gly Arg Leu Thr Ile Ser Leu Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Thr Ser Thr Trp Tyr Arg Gly Tyr Cys Gly Thr Asn Pro 
            100                 105                 110         


Asn Tyr Phe Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


<210>  14
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for adenylate kinase from Sulfolobus 
       acidocaldarius with unstructured linker and VHH sequence

<400>  14

Met Leu Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp Arg Asp Glu 
1               5                   10                  15      


Met Arg Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln Ile Asp Ala 
            20                  25                  30          


Ala Lys Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu Gly Tyr Leu 
        35                  40                  45              


Phe Ile Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly Tyr Leu Pro 
    50                  55                  60                  


Gly Leu Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser Val Ile Phe 
65                  70                  75                  80  


Leu Leu Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln Lys Arg Asp 
                85                  90                  95      


Thr Thr Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val Ile Leu Glu 
            100                 105                 110         


Thr Ile Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala Val Leu Ala 
        115                 120                 125             


Gly Ser Thr Val Lys Val Ile Val Asn Val Glu Gly Asp Pro Ser Ile 
    130                 135                 140                 


Ala Ala Asn Glu Ile Ile Arg Ser Met Lys Gln Ser Thr Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ser Glu Val 
                165                 170                 175     


Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gln Ser Leu 
            180                 185                 190         


Arg Leu Ser Cys Ala Thr Ser Gly Ala Thr Ser Ser Ser Asn Cys Met 
        195                 200                 205             


Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val Ala Val 
    210                 215                 220                 


Ile Asp Thr Gly Arg Gly Asn Thr Ala Tyr Ala Asp Ser Val Gln Gly 
225                 230                 235                 240 


Arg Leu Thr Ile Ser Leu Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln 
                245                 250                 255     


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys Ala Ala 
            260                 265                 270         


Asp Thr Ser Thr Trp Tyr Arg Gly Tyr Cys Gly Thr Asn Pro Asn Tyr 
        275                 280                 285             


Phe Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
    290                 295                 300         


<210>  15
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for adenylate kinase from Sulfolobus 
       acidocaldarius with helical linker and VHH sequence

<400>  15

Met Leu Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp Arg Asp Glu 
1               5                   10                  15      


Met Arg Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln Ile Asp Ala 
            20                  25                  30          


Ala Lys Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu Gly Tyr Leu 
        35                  40                  45              


Phe Ile Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly Tyr Leu Pro 
    50                  55                  60                  


Gly Leu Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser Val Ile Phe 
65                  70                  75                  80  


Leu Leu Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln Lys Arg Asp 
                85                  90                  95      


Thr Thr Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val Ile Leu Glu 
            100                 105                 110         


Thr Ile Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala Val Leu Ala 
        115                 120                 125             


Gly Ser Thr Val Lys Val Ile Val Asn Val Glu Gly Asp Pro Ser Ile 
    130                 135                 140                 


Ala Ala Asn Glu Ile Ile Arg Ser Met Lys Gln Ser Thr Leu Ala Glu 
145                 150                 155                 160 


Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
                165                 170                 175     


Ala Ala Lys Ala Ala Ala Gly Ser Glu Val Gln Leu Gln Ala Ser Gly 
            180                 185                 190         


Gly Gly Ser Val Gln Ala Gly Gln Ser Leu Arg Leu Ser Cys Ala Thr 
        195                 200                 205             


Ser Gly Ala Thr Ser Ser Ser Asn Cys Met Gly Trp Phe Arg Gln Ala 
    210                 215                 220                 


Pro Gly Lys Glu Arg Glu Gly Val Ala Val Ile Asp Thr Gly Arg Gly 
225                 230                 235                 240 


Asn Thr Ala Tyr Ala Asp Ser Val Gln Gly Arg Leu Thr Ile Ser Leu 
                245                 250                 255     


Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro 
            260                 265                 270         


Glu Asp Thr Ala Met Tyr Tyr Cys Ala Ala Asp Thr Ser Thr Trp Tyr 
        275                 280                 285             


Arg Gly Tyr Cys Gly Thr Asn Pro Asn Tyr Phe Ser Tyr Trp Gly Gln 
    290                 295                 300                 


Gly Thr Gln Val Thr Val Ser Ser 
305                 310         


<210>  16
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for VHH sequence with helical linker and 
       adenylate kinase from Sulfolobus acidocaldarius

<400>  16

Met Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly 
1               5                   10                  15      


Gln Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Ala Thr Ser Ser Ser 
            20                  25                  30          


Asn Cys Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly 
        35                  40                  45              


Val Ala Val Ile Asp Thr Gly Arg Gly Asn Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Gln Gly Arg Leu Thr Ile Ser Leu Asp Asn Ala Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Asp Thr Ser Thr Trp Tyr Arg Gly Tyr Cys Gly Thr Asn 
            100                 105                 110         


Pro Asn Tyr Phe Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser Ser Thr Leu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
    130                 135                 140                 


Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ala Gly Ser Met Leu 
145                 150                 155                 160 


Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp Arg Asp Glu Met Arg 
                165                 170                 175     


Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln Ile Asp Ala Ala Lys 
            180                 185                 190         


Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu Gly Tyr Leu Phe Ile 
        195                 200                 205             


Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly Tyr Leu Pro Gly Leu 
    210                 215                 220                 


Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser Val Ile Phe Leu Leu 
225                 230                 235                 240 


Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln Lys Arg Asp Thr Thr 
                245                 250                 255     


Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val Ile Leu Glu Thr Ile 
            260                 265                 270         


Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala Val Leu Ala Gly Ser 
        275                 280                 285             


Thr Val Lys Val Ile Val Asn Val Glu Gly Asp Pro Ser Ile Ala Ala 
    290                 295                 300                 


Asn Glu Ile Ile Arg Ser Met Lys 
305                 310         


<210>  17
<211>  301
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein sequence for VHH sequence with unstructured linker
       and adenylate kinase from Sulfolobus acidocaldarius

<400>  17

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Ser Val Gln Ala Gly Gln 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Ala Thr Ser Ser Ser Asn 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Val Ile Asp Thr Gly Arg Gly Asn Thr Ala Tyr Ala Asp Ser Val 
    50                  55                  60                  


Gln Gly Arg Leu Thr Ile Ser Leu Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Thr Ser Thr Trp Tyr Arg Gly Tyr Cys Gly Thr Asn Pro 
            100                 105                 110         


Asn Tyr Phe Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


Ser Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Ser Met Leu Ala Thr Ala Leu Lys Leu Gly Tyr Ala Lys Asp 
145                 150                 155                 160 


Arg Asp Glu Met Arg Lys Leu Ser Val Glu Lys Gln Lys Lys Leu Gln 
                165                 170                 175     


Ile Asp Ala Ala Lys Gly Ile Ala Glu Glu Ala Arg Ala Gly Gly Glu 
            180                 185                 190         


Gly Tyr Leu Phe Ile Asp Thr His Ala Val Ile Arg Thr Pro Ser Gly 
        195                 200                 205             


Tyr Leu Pro Gly Leu Pro Ser Tyr Val Ile Thr Glu Ile Asn Pro Ser 
    210                 215                 220                 


Val Ile Phe Leu Leu Glu Ala Asp Pro Lys Ile Ile Leu Ser Arg Gln 
225                 230                 235                 240 


Lys Arg Asp Thr Thr Arg Asn Arg Asn Asp Tyr Ser Asp Glu Ser Val 
                245                 250                 255     


Ile Leu Glu Thr Ile Asn Phe Ala Arg Tyr Ala Ala Thr Ala Ser Ala 
            260                 265                 270         


Val Leu Ala Gly Ser Thr Val Lys Val Ile Val Asn Val Glu Gly Asp 
        275                 280                 285             


Pro Ser Ile Ala Ala Asn Glu Ile Ile Arg Ser Met Lys 
    290                 295                 300     


<210>  18
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Helical linker

<400>  18

Leu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala 
1               5                   10                  15      


Lys Glu Ala Ala Ala Lys Ala Ala Ala 
            20                  25  


<210>  19
<211>  403
<212>  PRT
<213>  Thermotoga maritima

<400>  19

Met Arg Val Leu Val Ile Asn Ser Gly Ser Ser Ser Ile Lys Tyr Gln 
1               5                   10                  15      


Leu Ile Glu Met Glu Gly Glu Lys Val Leu Cys Lys Gly Ile Ala Glu 
            20                  25                  30          


Arg Ile Gly Ile Glu Gly Ser Arg Leu Val His Arg Val Gly Asp Glu 
        35                  40                  45              


Lys His Val Ile Glu Arg Glu Leu Pro Asp His Glu Glu Ala Leu Lys 
    50                  55                  60                  


Leu Ile Leu Asn Thr Leu Val Asp Glu Lys Leu Gly Val Ile Lys Asp 
65                  70                  75                  80  


Leu Lys Glu Ile Asp Ala Val Gly His Arg Val Val His Gly Gly Glu 
                85                  90                  95      


Arg Phe Lys Glu Ser Val Leu Val Asp Glu Glu Val Leu Lys Ala Ile 
            100                 105                 110         


Glu Glu Val Ser Pro Leu Ala Pro Leu His Asn Pro Ala Asn Leu Met 
        115                 120                 125             


Gly Ile Lys Ala Ala Met Lys Leu Leu Pro Gly Val Pro Asn Val Ala 
    130                 135                 140                 


Val Phe Asp Thr Ala Phe His Gln Thr Ile Pro Gln Lys Ala Tyr Leu 
145                 150                 155                 160 


Tyr Ala Ile Pro Tyr Glu Tyr Tyr Glu Lys Tyr Lys Ile Arg Arg Tyr 
                165                 170                 175     


Gly Phe His Gly Thr Ser His Arg Tyr Val Ser Lys Arg Ala Ala Glu 
            180                 185                 190         


Ile Leu Gly Lys Lys Leu Glu Glu Leu Lys Ile Ile Thr Cys His Ile 
        195                 200                 205             


Gly Asn Gly Ala Ser Val Ala Ala Val Lys Tyr Gly Lys Cys Val Asp 
    210                 215                 220                 


Thr Ser Met Gly Phe Thr Pro Leu Glu Gly Leu Val Met Gly Thr Arg 
225                 230                 235                 240 


Ser Gly Asp Leu Asp Pro Ala Ile Pro Phe Phe Ile Met Glu Lys Glu 
                245                 250                 255     


Gly Ile Ser Pro Gln Glu Met Tyr Asp Ile Leu Asn Lys Lys Ser Gly 
            260                 265                 270         


Val Tyr Gly Leu Ser Lys Gly Phe Ser Ser Asp Met Arg Asp Ile Glu 
        275                 280                 285             


Glu Ala Ala Leu Lys Gly Asp Glu Trp Cys Lys Leu Val Leu Glu Ile 
    290                 295                 300                 


Tyr Asp Tyr Arg Ile Ala Lys Tyr Ile Gly Ala Tyr Ala Ala Ala Met 
305                 310                 315                 320 


Asn Gly Val Asp Ala Ile Val Phe Thr Ala Gly Val Gly Glu Asn Ser 
                325                 330                 335     


Pro Ile Thr Arg Glu Asp Val Cys Ser Tyr Leu Glu Phe Leu Gly Val 
            340                 345                 350         


Lys Leu Asp Lys Gln Lys Asn Glu Glu Thr Ile Arg Gly Lys Glu Gly 
        355                 360                 365             


Ile Ile Ser Thr Pro Asp Ser Arg Val Lys Val Leu Val Val Pro Thr 
    370                 375                 380                 


Asn Glu Glu Leu Met Ile Ala Arg Asp Thr Lys Glu Ile Val Glu Lys 
385                 390                 395                 400 


Ile Gly Arg 
            


<210>  20
<211>  231
<212>  PRT
<213>  Thermotoga maritima

<400>  20

Met Arg Val Leu Val Lys Leu Ser Gly Glu Ala Leu Ser Gly Glu Gly 
1               5                   10                  15      


Gly Arg Gly Phe Asp Pro Glu Arg Val Asn Tyr Ile Val Asn Glu Ile 
            20                  25                  30          


Lys Ser Ala Ile Glu Glu Gly Phe Lys Ile Gly Ile Val Val Gly Ala 
        35                  40                  45              


Gly Asn Leu Phe Arg Gly Val Glu Leu Lys Asn Leu Thr Met Thr Arg 
    50                  55                  60                  


Ala Asp Gln Ile Gly Leu Leu Gly Thr Val Met Asn Ser Val Tyr Leu 
65                  70                  75                  80  


Lys Asp Ile Phe Glu Arg Ser Gly Leu Lys Ala Arg Ile Tyr Ser Gln 
                85                  90                  95      


Ile Val Asn Leu Pro Asp Val Glu Arg Val Asn Tyr Asp Ser Ile Glu 
            100                 105                 110         


Ser Ala Leu Arg Glu Asn Ser Ile Leu Ile Phe Ala Gly Gly Thr Ser 
        115                 120                 125             


Asn Pro Phe Phe Thr Thr Asp Thr Ala Ala Val Leu Arg Ala Gln Glu 
    130                 135                 140                 


Met Arg Ala Lys Leu Val Val Lys Ala Thr Lys Val Asp Gly Val Tyr 
145                 150                 155                 160 


Asp Lys Asp Pro Lys Lys Phe Pro Asp Ala Lys Lys Ile Pro His Leu 
                165                 170                 175     


Thr Phe Ser Glu Ala Met Lys Met Gly Leu Lys Val Met Asp Ala Glu 
            180                 185                 190         


Ala Phe Ala Leu Cys Lys Lys Leu Gly Ile Thr Val Lys Val Ile Asn 
        195                 200                 205             


Phe Phe Glu Pro Gly Thr Leu Leu Lys Ala Leu Lys Gly Glu Asp Val 
    210                 215                 220                 


Gly Ser Thr Val Val Pro Asp 
225                 230     


<210>  21
<211>  227
<212>  PRT
<213>  Sulfolobus acidocaldarius

<400>  21

Met Lys Leu Thr Leu Lys Val Ser Gly Lys Phe Phe Asp Glu Glu Asn 
1               5                   10                  15      


Ser Glu Asn Leu Ser Leu Leu Arg Asp Val Ile Ile Asp Leu Val Asn 
            20                  25                  30          


Asn Gly His Arg Val Ala Val Val Thr Gly Gly Gly Gly Thr Ala Arg 
        35                  40                  45              


Arg Tyr Ile Ser Met Gly Arg Lys Leu Asn Leu Asn Glu Ser His Leu 
    50                  55                  60                  


Asp Ile Leu Gly Ile Leu Val Ser Arg Leu Asn Ala Gln Leu Leu Leu 
65                  70                  75                  80  


Phe Ser Leu Asp Asn Ile Ala Tyr Pro Lys Val Pro Glu Ser Ile Glu 
                85                  90                  95      


Asp Phe Asn Glu Arg Trp Ala Ser Gly Lys Val Val Ile Thr Gly Gly 
            100                 105                 110         


Phe Gln Pro Gly Gln Ser Thr Ala Gly Val Ala Ala Leu Val Ser Glu 
        115                 120                 125             


Ile Ile Asn Ala Asp Tyr Leu Val Leu Ala Thr Asn Val Asn Gly Val 
    130                 135                 140                 


Tyr Thr Lys Asp Pro Gln Lys Phe Val Asp Ala Lys Leu Leu Pro Lys 
145                 150                 155                 160 


Leu Thr Val Ser Glu Leu Lys Thr Ile Leu Glu Gly Ser Gln Ser Val 
                165                 170                 175     


Asn Ala Gly Lys Tyr Glu Leu Leu Asp Pro Leu Ala Ile Lys Ile Val 
            180                 185                 190         


Glu Arg Ser Lys Ile Lys Val Leu Val Ile Asn Phe Lys Asp Leu Asn 
        195                 200                 205             


Lys Leu Pro Asn Ile Leu Lys Gly Asn Glu Ile Leu Gly Ser Val Val 
    210                 215                 220                 


Val Pro Glu 
225         


