
                                SEQUENCE LISTING

<110> Board of Trustees of Michigan State University
      Wilkerson, Curtis
      Ralph, John
      Withers, Saunia

<120> Hibiscus cannabinus
      Feruloyl-CoA:Monolignol Transferase

<130> 3000.064WO1

<150> 61/544,063          
<151> 2011-10-06   

<160> 17

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1326
<212> DNA
<213> Angelica sinensis

<400> 1
atgacgatca tggaggttca agttgtatct aagaagatgg taaagccatc agttccgact      60
cctgaccacc acaagacttg caaattgacg gcattcgatc agattgctcc tccggatcaa     120
gttcccatta tttacttcta caacagcagc aacatccaca atattcgcga gcaattggta     180
aaatccttgt ccgaaactct aaccaagttt tatccattag ctggaagatt tgttcaagat     240
ggtttctatg tcgattgtaa tgatgaaggg gtcttgtacg tagaagctga agttaacatt     300
ccgctaaacg aattcatcgg acaagcaaag aaaaatatac aacttatcaa tgatcttgtt     360
ccgaaaaaaa acttcaagga tattcattca tatgaaaatc caatagtggg attacagatg     420
agttatttca agtgtggtgg acttgctatt tgcatgtatc tttcgcatgt tgtagctgat     480
ggatatacag cagcagcatt cactaaagag tggtctaaca caaccaatgg catcatcaat     540
ggcgatcaac tagtttcttc ttctccgatt aacttcgaat tggcaactct agtcccagct     600
agagatttat cgacggtgat caagccagcc gtgatgccac catcaaagat caaggaaacc     660
aaggttgtca caaggaggtt tctgttcgat gaaaatgcga tatcagcttt caaagaccat     720
gtcatcaaat ccgaaagcgt taaccggcct acacgggtgg aagttgtgac atctgtgtta     780
tggaaggctc tgatcaacca gtctaagctt ccaagttcta cactatattt tcacctcaac     840
tttagaggga aaacaggcat caacacccca ccgctagata atcatttttc gctttgcgga     900
aacttttaca ctcaggttcc tacaaggttc agggggggaa atcaaacaaa acaggatttg     960
gaattgcatg aattggtcaa gttgttgaga ggaaagttgc gtaacactct gaagaattgc    1020
tccgaaatta acactgccga tgggctgttc ctggaagcag ctagtaattt caatattata    1080
caggaagatt tggaggacga acaagtggat gttcggattt ttacaacgtt gtgtaggatg    1140
cctttgtatg aaactgagtt tgggtgggga aaaccagaat gggttaccat tccagagatg    1200
catttggaga tagtgtttct tttggacact aaatgtggga ctggtattga ggcattagtg    1260
agcatggatg aagcagatat gcttcagttt gaacttgatc ccaccatctc tgctttcgct    1320
tcctag                                                               1326

<210> 2
<211> 441
<212> PRT
<213> Angelica sinensis

<400> 2
Met Thr Ile Met Glu Val Gln Val Val Ser Lys Lys Met Val Lys Pro
 1               5                  10                  15
Ser Val Pro Thr Pro Asp His His Lys Thr Cys Lys Leu Thr Ala Phe
            20                  25                  30
Asp Gln Ile Ala Pro Pro Asp Gln Val Pro Ile Ile Tyr Phe Tyr Asn
        35                  40                  45
Ser Ser Asn Ile His Asn Ile Arg Glu Gln Leu Val Lys Ser Leu Ser
    50                  55                  60
Glu Thr Leu Thr Lys Phe Tyr Pro Leu Ala Gly Arg Phe Val Gln Asp
65                  70                  75                  80
Gly Phe Tyr Val Asp Cys Asn Asp Glu Gly Val Leu Tyr Val Glu Ala
                85                  90                  95
Glu Val Asn Ile Pro Leu Asn Glu Phe Ile Gly Gln Ala Lys Lys Asn
            100                 105                 110
Ile Gln Leu Ile Asn Asp Leu Val Pro Lys Lys Asn Phe Lys Asp Ile
        115                 120                 125
His Ser Tyr Glu Asn Pro Ile Val Gly Leu Gln Met Ser Tyr Phe Lys
    130                 135                 140
Cys Gly Gly Leu Ala Ile Cys Met Tyr Leu Ser His Val Val Ala Asp
145                 150                 155                 160
Gly Tyr Thr Ala Ala Ala Phe Thr Lys Glu Trp Ser Asn Thr Thr Asn
                165                 170                 175
Gly Ile Ile Asn Gly Asp Gln Leu Val Ser Ser Ser Pro Ile Asn Phe
            180                 185                 190
Glu Leu Ala Thr Leu Val Pro Ala Arg Asp Leu Ser Thr Val Ile Lys
        195                 200                 205
Pro Ala Val Met Pro Pro Ser Lys Ile Lys Glu Thr Lys Val Val Thr
    210                 215                 220
Arg Arg Phe Leu Phe Asp Glu Asn Ala Ile Ser Ala Phe Lys Asp His
225                 230                 235                 240
Val Ile Lys Ser Glu Ser Val Asn Arg Pro Thr Arg Val Glu Val Val
                245                 250                 255
Thr Ser Val Leu Trp Lys Ala Leu Ile Asn Gln Ser Lys Leu Pro Ser
            260                 265                 270
Ser Thr Leu Tyr Phe His Leu Asn Phe Arg Gly Lys Thr Gly Ile Asn
        275                 280                 285
Thr Pro Pro Leu Asp Asn His Phe Ser Leu Cys Gly Asn Phe Tyr Thr
    290                 295                 300
Gln Val Pro Thr Arg Phe Arg Gly Gly Asn Gln Thr Lys Gln Asp Leu
305                 310                 315                 320
Glu Leu His Glu Leu Val Lys Leu Leu Arg Gly Lys Leu Arg Asn Thr
                325                 330                 335
Leu Lys Asn Cys Ser Glu Ile Asn Thr Ala Asp Gly Leu Phe Leu Glu
            340                 345                 350
Ala Ala Ser Asn Phe Asn Ile Ile Gln Glu Asp Leu Glu Asp Glu Gln
        355                 360                 365
Val Asp Val Arg Ile Phe Thr Thr Leu Cys Arg Met Pro Leu Tyr Glu
    370                 375                 380
Thr Glu Phe Gly Trp Gly Lys Pro Glu Trp Val Thr Ile Pro Glu Met
385                 390                 395                 400
His Leu Glu Ile Val Phe Leu Leu Asp Thr Lys Cys Gly Thr Gly Ile
                405                 410                 415
Glu Ala Leu Val Ser Met Asp Glu Ala Asp Met Leu Gln Phe Glu Leu
            420                 425                 430
Asp Pro Thr Ile Ser Ala Phe Ala Ser
        435                 440

<210> 3
<211> 49
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<220> 
<221> misc_feature   
<222> (1)...(49)
<223> n = A,T,G, or C

<400> 3
tagaggccga ggcggccgac atgttttgtt tttttttctt ttttttttn                  49

<210> 4
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 4
aaaaaagcag gcttcatgac gatcatggag gttcaagtt                             39

<210> 5
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 5
gtacaagaaa gctgggttct aggaagcgaa agcagagat                             39

<210> 6
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 6
ggggacaagt ttgtacaaaa aagcaggct                                        29

<210> 7
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 7
gggaccactt tgtacaagaa agctgggt                                         28

<210> 8
<211> 1389
<212> DNA
<213> Hibiscus cannabinus

<400> 8
atggcaaccc acagcactat catgttctca gtcgatagaa acgatgtcgt gtttgtcaaa      60
cccttcaaac ctacaccctc acaggttcta tctctctcca ccatcgacaa tgatcccaac     120
cttgagatca tgtgccatac tgtttttgtg tatcaagcca atgccgattt cgatgttaag     180
cccaaggatc cagcttccat aatccaggaa gcactctcca agctcttggt ttattactat     240
cccttagcgg ggaagatgaa gagggagacc gatggaaaac ttcgaatcgc ttgcactgcc     300
gacgatagcg tgcccttctt agtagccacc gccgattgca agctctcgtc gttgaaccac     360
ttggatggca tagatgttca taccgggaaa gaattcgcct tggattttgc atccgaatcc     420
gacggtggct attatcaccc tctggtcatg caggtgacga agttcatatg cggagggttc     480
accatcgctt tgagtttatc gcactcggtt tgtgatggct tcggtgcagc tcagatcttt     540
caagcattga ccgagctcgc aagtggcagg aacgagccct cggttaaacc cgtgtgggag     600
aggcaactat tagtggcgaa accggccgag gaaatccctc ggtcgattgt cgataaggac     660
ttgtcggcag cttcaccgta tctgccgaca accgacatag tccatgcctg cttttatgta     720
accgaggaga gtataaaaac actgaaaatg aatctgatca aagaaagcaa agatgagagt     780
ataaccagtc tcgaggtcct ttcagcctat atatggagag caaggtttag agcattgaaa     840
ttgagtccag ataaaaccac aatgctcggc atggccgtag gcatacgacg caccgtgaaa     900
ccacggttgc ccgaaggata ctacgggaat gctttcacct cggcaaatac ggccatgacc     960
gggaaggaac tcgaccaagg accgctctcg aaagctgtga aacaaatcaa ggagagcaaa    1020
aagcttgctt cggagaatga ctatatctgg aacttgatga gcattaacga gaagctgaga    1080
gaactgaatt cgaagttcga agcggccgcc ggttcaacca tggtcataac agattggagg    1140
cggttgggac tattggaaga tgtggatttt ggatggaaag gtagcgtaaa catgatacca    1200
ctgccgtgga acatgttcgg gtacgtggat ttggttcttt tattgcctcc ttgtaaactg    1260
gaccaatcga tgaaaggcgg tgctagagtg ttggtttcct ttcccacggc tgctattgcc    1320
aaattcaagg aagaaatgga tgctctcaaa catgataaca aggttgccgg cgatgctcta    1380
gtgatctag                                                            1389

<210> 9
<211> 462
<212> PRT
<213> Hibiscus cannabinus

<400> 9
Met Ala Thr His Ser Thr Ile Met Phe Ser Val Asp Arg Asn Asp Val
 1               5                  10                  15
Val Phe Val Lys Pro Phe Lys Pro Thr Pro Ser Gln Val Leu Ser Leu
            20                  25                  30
Ser Thr Ile Asp Asn Asp Pro Asn Leu Glu Ile Met Cys His Thr Val
        35                  40                  45
Phe Val Tyr Gln Ala Asn Ala Asp Phe Asp Val Lys Pro Lys Asp Pro
    50                  55                  60
Ala Ser Ile Ile Gln Glu Ala Leu Ser Lys Leu Leu Val Tyr Tyr Tyr
65                  70                  75                  80
Pro Leu Ala Gly Lys Met Lys Arg Glu Thr Asp Gly Lys Leu Arg Ile
                85                  90                  95
Ala Cys Thr Ala Asp Asp Ser Val Pro Phe Leu Val Ala Thr Ala Asp
            100                 105                 110
Cys Lys Leu Ser Ser Leu Asn His Leu Asp Gly Ile Asp Val His Thr
        115                 120                 125
Gly Lys Glu Phe Ala Leu Asp Phe Ala Ser Glu Ser Asp Gly Gly Tyr
    130                 135                 140
Tyr His Pro Leu Val Met Gln Val Thr Lys Phe Ile Cys Gly Gly Phe
145                 150                 155                 160
Thr Ile Ala Leu Ser Leu Ser His Ser Val Cys Asp Gly Phe Gly Ala
                165                 170                 175
Ala Gln Ile Phe Gln Ala Leu Thr Glu Leu Ala Ser Gly Arg Asn Glu
            180                 185                 190
Pro Ser Val Lys Pro Val Trp Glu Arg Gln Leu Leu Val Ala Lys Pro
        195                 200                 205
Ala Glu Glu Ile Pro Arg Ser Ile Val Asp Lys Asp Leu Ser Ala Ala
    210                 215                 220
Ser Pro Tyr Leu Pro Thr Thr Asp Ile Val His Ala Cys Phe Tyr Val
225                 230                 235                 240
Thr Glu Glu Ser Ile Lys Thr Leu Lys Met Asn Leu Ile Lys Glu Ser
                245                 250                 255
Lys Asp Glu Ser Ile Thr Ser Leu Glu Val Leu Ser Ala Tyr Ile Trp
            260                 265                 270
Arg Ala Arg Phe Arg Ala Leu Lys Leu Ser Pro Asp Lys Thr Thr Met
        275                 280                 285
Leu Gly Met Ala Val Gly Ile Arg Arg Thr Val Lys Pro Arg Leu Pro
    290                 295                 300
Glu Gly Tyr Tyr Gly Asn Ala Phe Thr Ser Ala Asn Thr Ala Met Thr
305                 310                 315                 320
Gly Lys Glu Leu Asp Gln Gly Pro Leu Ser Lys Ala Val Lys Gln Ile
                325                 330                 335
Lys Glu Ser Lys Lys Leu Ala Ser Glu Asn Asp Tyr Ile Trp Asn Leu
            340                 345                 350
Met Ser Ile Asn Glu Lys Leu Arg Glu Leu Asn Ser Lys Phe Glu Ala
        355                 360                 365
Ala Ala Gly Ser Thr Met Val Ile Thr Asp Trp Arg Arg Leu Gly Leu
    370                 375                 380
Leu Glu Asp Val Asp Phe Gly Trp Lys Gly Ser Val Asn Met Ile Pro
385                 390                 395                 400
Leu Pro Trp Asn Met Phe Gly Tyr Val Asp Leu Val Leu Leu Leu Pro
                405                 410                 415
Pro Cys Lys Leu Asp Gln Ser Met Lys Gly Gly Ala Arg Val Leu Val
            420                 425                 430
Ser Phe Pro Thr Ala Ala Ile Ala Lys Phe Lys Glu Glu Met Asp Ala
        435                 440                 445
Leu Lys His Asp Asn Lys Val Ala Gly Asp Ala Leu Val Ile
    450                 455                 460

<210> 10
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 10
aaaaaagcag gcttcatggc aacccacagc actatcat                              38

<210> 11
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 11
gtacaagaaa gctgggttct agatcactag agcatcgccg g                          41

<210> 12
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 12
ggggacaagt ttgtacaaaa aagcaggct                                        29

<210> 13
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 13
gggaccactt tgtacaagaa agctgggt                                         28

<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 14
cgcactcggt ttgtgatggc                                                  20

<210> 15
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic primer

<400> 15
ttcacagctt tcgagagcgg tc                                               22

<210> 16
<211> 374
<212> PRT
<213> Hibiscus cannabinus

<400> 16
Glu Ala Leu Ser Lys Leu Leu Val Tyr Tyr Tyr Pro Leu Ala Gly Lys
 1               5                  10                  15
Met Lys Arg Glu Thr Asp Gly Lys Leu Arg Ile Ala Cys Thr Ala Asp
            20                  25                  30
Asp Ser Val Pro Phe Leu Val Ala Thr Ala Asp Cys Lys Leu Ser Ser
        35                  40                  45
Leu Asn His Leu Asp Gly Ile Asp Val His Thr Gly Lys Glu Phe Ala
    50                  55                  60
Leu Asp Phe Ala Ser Glu Ser Asp Gly Gly Tyr Tyr His Pro Leu Val
65                  70                  75                  80
Met Gln Val Thr Lys Phe Ile Cys Gly Gly Phe Thr Ile Ala Leu Ser
                85                  90                  95
Leu Ser His Ser Val Cys Asp Gly Phe Gly Ala Ala Gln Ile Phe Gln
            100                 105                 110
Ala Leu Thr Glu Leu Ala Ser Gly Arg Asn Glu Pro Ser Val Lys Pro
        115                 120                 125
Val Trp Glu Arg Gln Leu Leu Val Ala Lys Pro Ala Glu Glu Ile Pro
    130                 135                 140
Arg Ser Ile Val Asp Lys Asp Leu Ser Ala Ala Ser Pro Tyr Leu Pro
145                 150                 155                 160
Thr Thr Asp Ile Val His Ala Cys Phe Tyr Val Thr Glu Glu Ser Ile
                165                 170                 175
Lys Thr Leu Lys Met Asn Leu Ile Lys Glu Ser Lys Asp Glu Ser Ile
            180                 185                 190
Thr Ser Leu Glu Val Leu Ser Ala Tyr Ile Trp Arg Ala Arg Phe Arg
        195                 200                 205
Ala Leu Lys Leu Ser Pro Asp Lys Thr Thr Met Leu Gly Met Ala Val
    210                 215                 220
Gly Ile Arg Arg Thr Val Lys Pro Arg Leu Pro Glu Gly Tyr Tyr Gly
225                 230                 235                 240
Asn Ala Phe Thr Ser Ala Asn Thr Ala Met Thr Gly Lys Glu Leu Asp
                245                 250                 255
Gln Gly Pro Leu Ser Lys Ala Val Lys Gln Ile Lys Glu Ser Lys Lys
            260                 265                 270
Leu Ala Ser Glu Asn Asp Tyr Ile Trp Asn Leu Met Ser Ile Asn Glu
        275                 280                 285
Lys Leu Arg Glu Leu Asn Ser Lys Phe Glu Ala Ala Ala Gly Ser Thr
    290                 295                 300
Met Val Ile Thr Asp Trp Arg Arg Leu Gly Leu Leu Glu Asp Val Asp
305                 310                 315                 320
Phe Gly Trp Lys Gly Ser Val Asn Met Ile Pro Leu Pro Trp Asn Met
                325                 330                 335
Phe Gly Tyr Val Asp Leu Val Leu Leu Leu Pro Pro Cys Lys Leu Asp
            340                 345                 350
Gln Ser Met Lys Gly Gly Ala Arg Val Leu Val Ser Phe Pro Thr Ala
        355                 360                 365
Ala Ile Ala Lys Phe Lys
    370

<210> 17
<211> 371
<212> PRT
<213> Angelica sinensis

<400> 17
Lys Ser Leu Ser Glu Thr Leu Thr Lys Phe Tyr Pro Leu Ala Gly Arg
 1               5                  10                  15
Phe Val Gln Asp Gly Phe Tyr Val Asp Cys Asn Asp Glu Gly Val Leu
            20                  25                  30
Tyr Val Glu Ala Glu Val Asn Ile Pro Leu Asn Glu Phe Ile Gly Gln
        35                  40                  45
Glu Lys Lys Asn Ile Gln Leu Ile Asn Asp Leu Val Pro Lys Lys Asn
    50                  55                  60
Phe Lys Asp Ile His Ser Tyr Glu Asn Pro Ile Val Gly Leu Gln Met
65                  70                  75                  80
Ser Tyr Phe Lys Cys Gly Gly Leu Ala Ile Cys Met Tyr Leu Ser His
                85                  90                  95
Val Val Ala Asp Gly Tyr Thr Ala Ala Ala Phe Thr Lys Glu Trp Ser
            100                 105                 110
Asn Thr Thr Asn Gly Ile Ile Asn Gly Asp His Leu Val Ser Ser Ser
        115                 120                 125
Pro Ile Asn Phe Asp Leu Ala Thr Leu Val Pro Thr Arg Asp Leu Ser
    130                 135                 140
Thr Val Ile Lys Pro Ala Val Met Pro Pro Ser Lys Ile Lys Glu Thr
145                 150                 155                 160
Lys Val Val Thr Arg Arg Phe Leu Phe Asp Glu Asn Ala Ile Ser Ala
                165                 170                 175
Phe Lys Asp His Val Ile Lys Ser Glu Ser Val Asn Arg Pro Thr Arg
            180                 185                 190
Val Glu Val Val Thr Ser Val Leu Trp Lys Ala Leu Ile Asn Gln Ser
        195                 200                 205
Lys Leu Pro Ser Ser Thr Leu Tyr Phe His Leu Asn Phe Arg Gly Lys
    210                 215                 220
Thr Gly Ile Asn Thr Pro Pro Leu Asp Asn His Phe Ser Leu Cys Gly
225                 230                 235                 240
Asn Phe Tyr Thr Gln Val Pro Thr Arg Phe Arg Gly Glu Asn Gln Thr
                245                 250                 255
Lys Gln Asp Leu Glu Leu His Glu Leu Val Lys Leu Leu Arg Gly Lys
            260                 265                 270
Leu Arg Asn Thr Leu Lys Asn Cys Ser Glu Ile Asn Thr Ala Asp Gly
        275                 280                 285
Leu Phe Leu Glu Ala Ala Ser Asn Phe Asn Ile Ile Gln Glu Asp Leu
    290                 295                 300
Glu Asp Glu Gln Val Asp Val Arg Ile Phe Thr Thr Leu Cys Arg Met
305                 310                 315                 320
Pro Leu Tyr Glu Thr Glu Leu Gly Trp Gly Lys Pro Glu Trp Val Thr
                325                 330                 335
Ile Pro Glu Met His Leu Glu Ile Val Phe Leu Leu Asp Thr Lys Cys
            340                 345                 350
Gly Thr Gly Ile Glu Ala Leu Val Ser Met Asp Glu Ala Asp Met Leu
        355                 360                 365
Gln Phe Glu
    370
