                         SEQUENCE LISTING

<110>  MASSACHUSETTS INSTITUTE OF TECHNOLOGY
       UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
       Ting, Alice Y.
       Liu, Daniel
       Baker, David
       Nivon, Lucas
 
<120>  METHODS AND COMPOSITIONS FOR POLYPEPTIDE AND PROTEIN LABELING USING LIPOIC ACID LIGASES SPECIFIC TO RESORUFIN

<130>  M0656.70237WO00

<140>  US 13/267,761
<141>  2011-10-06

<150>  US 11/983,605
<151>  2007-11-09

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  1

Gly Leu Asn Asp Ile Phe Glu Ala Asp Lys Ala Glu Trp His Glu 
1               5                   10                  15  


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  2

Gly Asp Thr Leu Cys Ile Val Glu Ala Asp Lys Ala Met Asn Gln Ile 
1               5                   10                  15      


Glu 
    


<210>  3
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  3

Gly Asp Thr Leu Cys Ile Val Glu Ala Asp Lys Ala Ser Met Glu Ile 
1               5                   10                  15      


Pro 
    


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  4

Glu Gln Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val 
1               5                   10                  15      


Pro 
    


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  5

Asp Asp Val Leu Cys Glu Val Gln Asn Asp Lys Ala Val Val Glu Ile 
1               5                   10                  15      


Pro 
    


<210>  6
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  6

Asp Glu Val Leu Val Glu Ile Glu Thr Asp Lys Val Val Leu Glu Val 
1               5                   10                  15      


Pro 
    


<210>  7
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  7

Gly Asp Asp Cys Ala Val Ala Glu Ser Val Lys Ala Ala Ser Asp Ile 
1               5                   10                  15      


Tyr 
    


<210>  8
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  8

Asp Glu Val Leu Val Glu Ile Glu Thr Asp Lys Ala Val Leu Glu Val 
1               5                   10                  15      


Pro 
    


<210>  9
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  9

Asp Glu Val Leu Val Glu Ile Glu Thr Asp Lys Ala Val Leu Glu Val 
1               5                   10                  15      


Pro 
    


<210>  10
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  10

Asp Glu Val Leu Val Glu Ile Glu Thr Asp Lys Ala Val Leu Glu Val 
1               5                   10                  15      


Pro Gly Gly Glu Glu Glu 
            20          


<210>  11
<211>  337
<212>  PRT
<213>  E. coli

<400>  11

Ser Thr Leu Arg Leu Leu Ile Ser Asp Ser Tyr Asp Pro Trp Phe Asn 
1               5                   10                  15      


Leu Ala Val Glu Glu Cys Ile Phe Arg Gln Met Pro Ala Thr Gln Arg 
            20                  25                  30          


Val Leu Phe Leu Trp Arg Asn Ala Asp Thr Val Val Ile Gly Arg Ala 
        35                  40                  45              


Gln Asn Pro Trp Lys Glu Cys Asn Thr Arg Arg Met Glu Glu Asp Asn 
    50                  55                  60                  


Val Arg Leu Ala Arg Arg Ser Ser Gly Gly Gly Ala Val Phe His Asp 
65                  70                  75                  80  


Leu Gly Asn Thr Cys Phe Thr Phe Met Ala Gly Lys Pro Glu Tyr Asp 
                85                  90                  95      


Lys Thr Ile Ser Thr Ser Ile Val Leu Asn Ala Leu Asn Ala Leu Gly 
            100                 105                 110         


Val Ser Ala Glu Ala Ser Gly Arg Asn Asp Leu Val Val Lys Thr Val 
        115                 120                 125             


Glu Gly Asp Arg Lys Val Ser Gly Ser Ala Tyr Arg Glu Thr Lys Asp 
    130                 135                 140                 


Arg Gly Phe His His Gly Thr Leu Leu Leu Asn Ala Asp Leu Ser Arg 
145                 150                 155                 160 


Leu Ala Asn Tyr Leu Asn Pro Asp Lys Lys Lys Leu Ala Ala Lys Gly 
                165                 170                 175     


Ile Thr Ser Val Arg Ser Arg Val Thr Asn Leu Thr Glu Leu Leu Pro 
            180                 185                 190         


Gly Ile Thr His Glu Gln Val Cys Glu Ala Ile Thr Glu Ala Phe Phe 
        195                 200                 205             


Ala His Tyr Gly Glu Arg Val Glu Ala Glu Ile Ile Ser Pro Asn Lys 
    210                 215                 220                 


Thr Pro Asp Leu Pro Asn Phe Ala Glu Thr Phe Ala Arg Gln Ser Ser 
225                 230                 235                 240 


Trp Glu Trp Asn Phe Gly Gln Ala Pro Ala Phe Ser His Leu Leu Asp 
                245                 250                 255     


Glu Arg Phe Thr Trp Gly Gly Val Glu Leu His Phe Asp Val Glu Lys 
            260                 265                 270         


Gly His Ile Thr Arg Ala Gln Val Phe Thr Asp Ser Leu Asn Pro Ala 
        275                 280                 285             


Pro Leu Glu Ala Leu Ala Gly Arg Leu Gln Gly Cys Leu Tyr Arg Ala 
    290                 295                 300                 


Asp Met Leu Gln Gln Glu Cys Glu Ala Leu Leu Val Asp Phe Pro Glu 
305                 310                 315                 320 


Gln Glu Lys Glu Leu Arg Glu Leu Ser Ala Trp Met Ala Gly Ala Val 
                325                 330                 335     


Arg 
    


<210>  12
<211>  1245
<212>  DNA
<213>  E. coli

<400>  12
gttaaccgcc agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat       60

tactacaaga aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga      120

cccgtggttt aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg      180

cgttctgttt ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg      240

gaaagagtgt aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtag      300

cggtggcggc gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa      360

gccggagtac gataaaacta tctccacgtc gattgtgctc aatgcgctga acgcgctcgg      420

cgtcagcgcc gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg      480

caaagtctca ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt      540

gctactcaat gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact      600

ggcggcgaaa ggcattacgt cggtacgttc ccgcgtgacc aacctcaccg agctgttgcc      660

ggggatcacc catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg      720

cgagcgcgtg gaagcggaaa tcatctcccc gaacaaaacg ccagacttgc caaacttcgc      780

cgaaaccttt gcccgccaga gtagctggga atggaacttc ggtcaggctc cggcattctc      840

gcatctgctg gatgaacgct ttacctgggg cggcgtggaa ctgcatttcg acgttgaaaa      900

aggccatatc acccgcgccc aggtgtttac cgacagcctc aaccccgcgc cgctggaagc      960

cctcgccgga cgactgcaag gctgcctgta ccgcgcagat atgctgcaac aggagtgcga     1020

agcgctgttg gttgacttcc cggaacagga aaaagagcta cgggagttat cggcatggat     1080

ggcgggggct gtaaggtagt tacccgccca tgcgggcaac tttctcttcg atttgccgga     1140

ttttttccgg catttcttgc gcaatgtggc gaaacgcctc gctgatacgc ggatctctgg     1200

cgaccgtttg ccagaactcg tgcgccagcg ggatcgccgt taacc                     1245


<210>  13
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  13
aaaagctagc ggcctgaacg acatcttcga agccgacaaa gctgaatggc acgagggcgg       60

tgaggaggag tacgccgtgg                                                   80


<210>  14
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  14
aaaagctagc ggcgataccc tgtgcatcgt tgaagccgac aaagctgaaa accagatcga       60

aggcggtgag gaggagtacg ccgtgg                                            86


<210>  15
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  15
aaaagctagc ggcgataccc tgtgcatcgt tgaagccgac aaagcttcta tggaaatccc       60

gggcggtgag gaggagtacg ccgtgg                                            86


<210>  16
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  16
aaaagctagc gaacagtcgc tgatcaccgt agaaggcgac aaagcttcta tggaagttcc       60

gggcggtgag gaggagtacg ccgtgg                                            86


<210>  17
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  17
aaaagctagc gacgatgtac tgtgcgaagt acagaacgac aaagctgtag ttgaaatccc       60

gggcggtgag gaggagtacg ccgtgg                                            86


<210>  18
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  18
aaaagctagc gacgaagtac tggttgaaat cgaaaccgac aaagtagttc tggaagtacc       60

gggcggtgag gaggagtacg ccgtgg                                            86


<210>  19
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  19
aaaagctagc ggcgatgact gcgctgttgc tgaatctgta aaagctgcct cggacatcta       60

tggcggtgag gaggagtacg ccgtgg                                            86


<210>  20
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  20
atcgaaaccg acaaagcagt tctggaagta ccgggc                                 36


<210>  21
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  21
aaaacatatg gaggaggagt acgccgtgg                                         29


<210>  22
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  22
ttttggatcc tcttacggta cttccagaac tgctttgtcg gtttcgattt caaccagtac       60

ttcgtcgcta gcatccttgc ggctcgcctc gtac                                   94


<210>  23
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  23
ttttggatcc tcttactcct cctcaccgcc cggtacttcc agaactgctt tgtc             54


<210>  24
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  24
aaaaactagt cgggctgacg aagtactggt tgaaatcgaa accgacaaag cagttctgga       60

agtaccggca tcagcagacg gcgctagcaa aa                                     92


<210>  25
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  25
ggttgaaatc gaaaccgacg ccgcagttct ggaagtaccg g                           41


<210>  26
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  26
cgaaggcgac cgcgcagtct caggctcgg                                         29


<210>  27
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  27
aaaaaagatc tggcggcgac gaagtactgg ttgaaatcga aaccgacaaa gcagttctgg       60

aagtaccggg cggtgaggag gagggcgcgc caaaaa                                 96


<210>  28
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  28
ggttgaaatc gaaaccgacg ccgcagttct ggaagtaccg g                           41


<210>  29
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  29
cgggcggtga ggaggagact gtgagcaagg gcgaggag                               38


<210>  30
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  30
gtacctccag aactgctttg tcggtttcga tttcaaccag tacttcgtca cttctgtcgc       60

caactgcag                                                               69


<210>  31
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  31
ggaggtaccg gcatcagcag acggcggggg agaattcgac agatgtg                     47


<210>  32
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  32

Gly Phe Glu Ile Asp Lys Val Trp Tyr Asp Leu Asp Ala 
1               5                   10              


<210>  33
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  33

Asp Glu Val Leu Val Glu Ile Glu Thr Asp Lys Ala Val Leu Glu Val 
1               5                   10                  15      


Pro Ala Ser Ala Asp Gly 
            20          


<210>  34
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  34
ttttgaattc ggatccttgc ggctcgcctc gtac                                   34


