                         SEQUENCE LISTING

<110>  Dow AgroSciences LLC
       Narva, Kenneth E.
       Li, Huarong
       Geng, Chaoxian
       Larrinua, Ignacio
       Olson, Monica B,
       Elango, Navin B,
 
<120>  NUCLEIC ACID MOLECULES THAT TARGET RPS6 AND CONFER RESISTANCE TO 
       COLEOPTERAN PESTS

<130>  71925-US-NP

<140>  2971-10733.1US
<141>  2011-10-06

<160>  110    

<170>  PatentIn version 3.5

<210>  1
<211>  1508
<212>  DNA
<213>  Diabrotica virgifera

<400>  1
cgcaaaaaag tgttgtttgg tttgtagtta aaaggctctg taaaaatcat taaaaatccg       60

agccatcttt tagttttaag tttcttaaat attgtcaaag agtatcacaa ggatttctca      120

aatggcagaa gcagataaat tgaatatcga cagtataata gcccgtttat tagaagtgcg      180

tggagcaaga ccaggcaaaa atgtacaact cacagaaaat gaaattaggg ggctctgttt      240

aaaatctaga gagatcttcc ttagccagcc gattttgttg gaacttgaag ctcctctgaa      300

gatttgcggt gatatacatg gtcagtacta tgacttgctt cgtctctttg aatatggagg      360

tttccctccc gaatcaaact acttattttt gggagattat gtagatcgtg gtaaacaatc      420

attggaaacc atctgcttac ttctcgctta caaaattaaa tacccagaaa actttttcct      480

actcagaggc aaccacgaat gcgcatcaat taatcgtata tatggattct atgatgaatg      540

caaaagaagg tataacatca agttgtggaa aacttttacg gactgtttca attgcctacc      600

tgtagcagcc atcgtcgatg aaaaaatttt ctgttgccat ggtggtttaa gtccggacct      660

acaatcaatg gaacaaatta gaagaattat gagaccgact gatgtacctg accaagggct      720

tctttgtgac cttttatggt ctgatccaga caaagaccag atgggatggg gagaaaacga      780

tagaggagtt agttttactt ttggtgctga agttgtagga aagttcttgc acaaacacga      840

ttttgatttg atatgtcgag cgcatcaagt cgtagaagat ggatatgaat tcttcgccaa      900

aagacagtta gtcacactgt ttagtgcgcc aaattattgt ggagagtttg ataacgcagg      960

tgcgatgatg tcggtggatg agacactaat gtgtagtttt caaattttaa agccagcaga     1020

caagaggaaa ttccagtaca acatgaacgc aggcagacct gtgacgccgc caagaggcgc     1080

aacgaataaa aacaagaaga agtgaatgaa taatatattt ataaggttag gtttagtcgc     1140

aacataaaca tgttcaaaac attttaaata ctaaaatttt ctaaaggtta caaagattca     1200

agataaatta agattttctt catgtttttg ttggttgttt tataggttag gatagtaaac     1260

tatataataa taaagttctc aatattgtta aaaagaagtg aatgttagta tttaaaatgt     1320

tcgattattt cggccgtttt actttatttt atatctgata ttactagaaa agggtgatat     1380

ctatgaaccc agacaactaa acgttcgatt tgaacaaatg aaaatttatt gaaaacatta     1440

atcctcacaa ccttgcttat ttaattaaag aacaagatca gtaatacatt aaagtctatc     1500

attaataa                                                              1508


<210>  2
<211>  2519
<212>  DNA
<213>  Diabrotica virgifera

<400>  2
caaaggtttc gtttcaaact tcacaccgat aaagacttgt ttgttcttgt cagtgtcagt       60

tctggcggta aaatatttgc ggtatacaca ttttttacgt cgtacgtaat ttgcaggggt      120

tgattactga tctttatttg ataatttgtt tatttatttt gcaacataag caaaatgcgt      180

tcgcctcaaa cctataacat gtcagaagga tcactccaga caatcatgtc tggaggtgaa      240

tttccaaatc ccattatgca agttttgggt agcaaaaaga taaacgccgg attgggtgat      300

aaagaaagaa ttcgtatttt actgtcagat ggaaaataca ctatttcttt tgccatgcta      360

acagcccaaa ttaatgatcg acttggtcca aatggtgtgg aaacttttag cattatacaa      420

atagatagat atgttacgag tatcatcaac aattctggga aaggagaagc acgagtactt      480

ttaatcctcg atatgcatgt tgttgtccct ggaactgaag ttacagaaaa agtaggctct      540

cccattcccc taccaactga tgctgacgca gctaaaggct ctactgccgc tccagctaca      600

aacaattcca ttaagaatgt aactgttgct aaaccaaaca tcagtaatgg caatggcaca      660

actgcaatga atgccagtac taatgatgat atagccacac atatgatcca tcctatttca      720

agtctcacac cttaccaaaa cagatggact atcaaagcga gaattactaa taaacctcca      780

ataagaacgt ggtcaaattc tagaggggaa ggaaaattat ttagttttga tctggtggat      840

gaaagtggcg aaatccgttg cacagctttt aaagaaatgg ttgataaatt ctatgattac      900

ctgcaggtgg ataaagtata ttacatcaac aaatgtcaac ttaaacaagc caacaaacag      960

tacagcactc taaaacatga gtatgaaatg actgttacgc atgatactgt cattaaagaa     1020

tgccttgatg cagattctac aatacccacc acacagtata actttgttcc tatagataaa     1080

attgctgata aagaagtaaa ttctgttgta gatgtaatag gtattgccaa aagtgtcagt     1140

gaattacaaa cattccaagc aagatcaaca ggaagagaat tgaaaaagaa agaagttgtc     1200

ttggttgatc agtcacaaac agctatatcg ttaacacttt ggggccaaga agccgaaaat     1260

tttgatggta ccaataatcc tgtcgtagtt ataaaaagtg ccaaaattgg cgagtttgga     1320

ggtggcaaga atttaactac tcttgttagc agcactgtaa aaataaatcc cgaattaaaa     1380

gaatgttaca ggatcagagg atggtacgac agtgagggtg aaaatctgaa tgcaaagaat     1440

attagtgcca gagttggatc ctcgaatatg tctgccactt ggatgacctt taaggaagtt     1500

aaagatcaaa aattaggatc atctgaaaaa ggtgattatt ataaagctat tgctactgtt     1560

cttcttgtca aagccgataa tattgtgtat agagcttgtc ccaccgctga atgtaataag     1620

aaagttgttg atatggaaaa tagtatgtac agatgtgaaa aatgtaatag agaatttcca     1680

aatttcaaat acagactgtt agccagcatg aatgttggag accacacagg aaaccaatgg     1740

gttagcatgt tcagttcaga agccgaaaaa attctgggga tgactgctga ggaagtagga     1800

cagaccttgg aacacaataa agaagaaata gccaacatcg tagatagagc tcattttaaa     1860

gtatttagtc ttacttgcag ggcaaaaatt gagacttaca atgatgaagc tcgtttaaaa     1920

actgtttgta taagagtcga tccaattaat tatgaggagt atagtgcatt gctcacagaa     1980

aaaattcagc agttaacagg cgaatctcat gattagatat acaccaacac tacagctatg     2040

ctattatttc tagttctttt tttttttaga aaatatcgtt aagaaatctg tgttttgtat     2100

ttatttttta taaacagtga atcagtgaat aagattttat tagaaaggta ctgtataaat     2160

aaaaatctgt atgttcacaa tatttttatt tatttaaata tacattggta caaaataaaa     2220

tatatattcg taacaactat attattgttt attattgttt attcttaagc cccatcatct     2280

aaagaggttc taaatgtgct tgttttcttg catacgcacc taaacaagct aaaattagta     2340

ttacactcat aaataatcct attaataagg ctaaagtatc tccaaaatca aacattttgc     2400

tgtattattg agtgtttaaa taattacatc aaaataaaat attttttatt ttttgcttgt     2460

cttgtatgtt tatttacgtt ttacttgtca atcagctgtc tatttcttct ttttaatta      2519


<210>  3
<211>  772
<212>  DNA
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (751)..(751)
<223>  n is a, c, g, or t

<400>  3
aattcaagct gccgcaaaaa agtgttgttt ggtttgtagt taaaaggctc tgtaaaaatc       60

attaaaaatc cgagccatct tttagtttta agtttcttaa atattgtcaa agagtatcac      120

aaggatttct caaatggcag aagcagataa attgaatatc gacagtataa tagcccgttt      180

attagaagtg cgtggagcaa gaccaggcaa aaatgtacaa ctcacagaaa atgaaattag      240

ggggctctgt ttaaaatcta gagagatctt ccttagccag ccgattttgt tggaacttga      300

agctcctctg aagatttgcg gtgatataca tggtcagtac tatgacttgc ttcgtctctt      360

tgaatatgga ggtttccctc ccgaatcaaa ctacttattt ttgggagatt atgtagatcg      420

tggtaaacaa tcattggaaa ccatctgctt acttctcgct tacaaaatta aatacccaga      480

aaactttttc ctactcagag gcaaccacga atgcgcatca attaatcgta tatatggatt      540

ctatgatgaa tgcaaaagaa ggtataacat caagttgtgg aaaactttta cggactgttt      600

caattgccta cctgtagcag ccatcgtcga tgaaaaaatt ttctgttgcc atggtggttt      660

aagtccggac ctacaatcaa tggaacaaat tagaagratt aatagagacc gactgatgta      720

cctgaccaag gstttctttg tgacctttta nggtctgatc cagacaaaga cc              772


<210>  4
<211>  632
<212>  DNA
<213>  Diabrotica virgifera

<400>  4
ataatttgca ggggttgatt actgatcttt atttgattaa tttgtttatt tatttttgca       60

acataagcaa aatgcgttcg cctcaaacct ataacatgtc agaaggatca ctccagacaa      120

tcatgtctgg aggtgaattt ccaaatccca ttatgcaagt tttgggtagc aaaaagataa      180

acgccggatt gggtgataaa gaaagaattc gtattttact gtcagatgga aaatacacta      240

tttcttttgc catgctaaca gcccaaatta atgatcgact tggtccaaat ggtgtggaaa      300

ctttttagca ttatacaaat agatagatat gttacgagta tcatcaacaa ttctgggaaa      360

ggagaagcac gagtactttt aatcctcgat atgcatgttg ttgtccctgg aactgaagtt      420

acagaaaaag taggctctcc cattccccta ccaactgatg ctgackcagc taaaggctct      480

actgccgctc cagctacaaa caattccatt aagaatgtaa ctgttgctaa accaaacatc      540

agtaatggca atggcacaac tgcaatgaat gccagtacta atgatgatat agccacacat      600

atgatccatc ctatttcaag tctcacacct ta                                    632


<210>  5
<211>  509
<212>  DNA
<213>  Diabrotica virgifera

<400>  5
atggtcaaat tctagagggg aaggaaaatt atttagtttt gatctggtgg atgaaagtgg       60

cgaaatccgt tgcacagctt ttaaagaaat ggttgataaw ttctatgatt acctgcaggt      120

ggataaagta tattacatca acaaatgtca acttaaacaa gccaacaaac agtacagcac      180

tctaaaacat gagtatgaaa tgactgttac gcatgatact gtcattaaag aatgccttga      240

tgcagattct acaataccca ccacacagta taactttgtt cctatagata aaattgctga      300

taaagaagta aattctgttg tagatgtaat aggtattgcc aaaagtgtca gtgaattaca      360

aacattccaa gcaagatcaa caggaagaga attgaaaaag aaagaagttg tcttggttga      420

tcagtcacaa acagctatat cgttaacact ttggggccaa gaagccgaaa attttgatgg      480

taccaataat cctgtcgtag ttataaaaa                                        509


<210>  6
<211>  716
<212>  DNA
<213>  Diabrotica virgifera

<400>  6
aactcttgtt agcagcamtr taaaaataaa tcccgaatta aaagaatgtt acaggatcag       60

aggatggtac gacagtgagg gtgaaaatct gaatgcaaag aatattagtg ccagagttgg      120

atcctcgaat atgtctgcca cttggatgac ctttaaggaa gttaaagatc aaaaattagg      180

atcatctgaa aaaggtgatt attataaagc tattgctact gttcttcttg tcaaagccga      240

taatattgtg tatagagctt gtcccaccgc tgaatgtaat aagaaagttg ttgatatgga      300

aaatagtatg tacagatgtg aaaaatgtaa tagagaattt ccaaatttca aatacagact      360

gttagccagc atgaatgttg gagaccacac aggaaaccaa tgggttagca tgttcagttc      420

agaagccgaa aaaattctgg ggatgactgc tgaggaagta ggacagacct tggaacacaa      480

taaagaagaa atagccaaca tcgtagatag agctcatttt aaagtattta gtcttacttg      540

cagggcaaaa attgagactt acaatgatga agctcgttta aaaactgttt gtataagagt      600

cgatccaatt aattatgagg agtatagtgc attgctcaca gaaaaaattc agcagttaac      660

aggcgaatct catgattaga tatacaccaa cactacagct atgctattat ttctag          716


<210>  7
<211>  933
<212>  DNA
<213>  Diabrotica virgifera

<400>  7
attaattttc tgaaatatcc tttttgaaac atggcagttc catgtgcaca ctaacgagaa       60

gtttttcccg tatttagtgt aatttgccaa aaataaagtg tgaaatagta gttttcgagt      120

gcaaaataag tcaatatgaa gttgaacgta tcgtacccgg ccacgggttg ccaaaaactt      180

ttcgaagttg ttgacgaaca caaaattcgt atcttttacg aaaaacgcat gggtcaagaa      240

gttgaggctg atgctcttgg tgacgaatgg aagggctaca tcttgaaaat atctggaggt      300

aacgacaaac aaggattccc catgaaacaa ggtgttctta caaacggtag agtaagactt      360

ttactttcaa aaggtcactc ctgctacaga cccagacgta ccggtgaacg taaaaggaaa      420

tcagttcgtg ggtgcattgt tgatgggaac ctcagcgtgt tggccctagt cattgtaaga      480

aaaggagaac aagaaattcc cggacttact gacaccacca tcccacgtcg cctgggaccc      540

aagagagcat ccagaatccg taagctcttc aaccttagca aagaagacga tgtacgtcaa      600

tatgtagtaa agagaccttt ggcccaaaaa gaaggtaaga agttaagaac caaggccccc      660

aaaatccaac gtcttattac acccgttgtt ttgcaaagaa aacgtcatcg tcttgctttg      720

aagaagaaga ggtgccttaa acgtaaagaa caagaagatg catatgctaa actattggct      780

caacgtaaga aggaatccaa ggctcgtcgt gagatgttga agaggcgtag gtctgccagt      840

atgcgtgata gtaaatccag cacgcagagt ggtcagaagt aaattgtaat tttttatatt      900

ttaagacaat gtatgaaata aacgttgttg ctt                                   933


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Escherichia coli T7 bacteriophage

<400>  8
ttaatacgac tcactatagg gaga                                              24


<210>  9
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  9
ttaatacgac tcactatagg gagacaaatg gcagaagcag ataaattg                    48


<210>  10
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  10
ctaatttgtt ccattgattg taggtcc                                           27


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDR Primer

<400>  11
caaatggcag aagcagataa attg                                              24


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  12
ttaatacgac tcactatagg gagactaatt tgttccattg attgtaggtc c                51


<210>  13
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  13
ttaatacgac tcactatagg gagaatggtc aaattctaga ggggaa                      46


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  14
ctacgacagg attattggta ccatc                                             25


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  15
atggtcaaat tctagagggg aa                                                22


<210>  16
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  16
ttaatacgac tcactatagg gagactacga caggattatt ggtaccatc                   49


<210>  17
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  17
ttaatacgac tcactatagg gagatcccga attaaaagaa tgttacagga                  50


<210>  18
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  18
cgagcttcat cattgtaagt ctcaat                                            26


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  19
tcccgaatta aaagaatgtt acagga                                            26


<210>  20
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  20
ttaatacgac tcactatagg gagacgagct tcatcattgt aagtctcaat                  50


<210>  21
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  21
ttaatacgac tcactatagg gagatcaata tgaagttgaa cgtatcg                     47


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  22
atgctctctt gggtcccagg                                                   20


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  23
tcaatatgaa gttgaacgta tcg                                               23


<210>  24
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  24
ttaatacgac tcactatagg gagaatgctc tcttgggtcc cagg                        44


<210>  25
<211>  563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  25
caaatggcag aagcagataa attgaatatc gacagtataa tagcccgttt attagaagtg       60

cgtggagcaa gaccaggcaa aaatgtacaa ctcacagaaa atgaaattag ggggctctgt      120

ttaaaatcta gagagatctt ccttagccag ccgattttgt tggaacttga agctcctctg      180

aagatttgcg gtgatataca tggtcagtac tatgacttgc ttcgtctctt tgaatatgga      240

ggtttccctc ccgaatcaaa ctacttattt ttgggagatt atgtagatcg tggtaaacaa      300

tcattggaaa ccatctgctt acttctcgct tacaaaatta aatacccaga aaactttttc      360

ctactcagag gcaaccacga atgcgcatca attaatcgta tatatggatt ctatgatgaa      420

tgcaaaagaa ggtataacat caagttgtgg aaaactttta cggactgttt caattgccta      480

cctgtagcag ccatcgtcga tgaaaaaatt ttctgttgcc atggtggttt aagtccggac      540

ctacaatcaa tggaacaaat tag                                              563


<210>  26
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  26
atggtcaaat tctagagggg aaggaaaatt atttagtttt gatctggtgg atgaaagtgg       60

cgaaatccgt tgcacagctt ttaaagaaat ggttgataaw ttctatgatt acctgcaggt      120

ggataaagta tattacatca acaaatgtca acttaaacaa gccaacaaac agtacagcac      180

tctaaaacat gagtatgaaa tgactgttac gcatgatact gtcattaaag aatgccttga      240

tgcagattct acaataccca ccacacagta taactttgtt cctatagata aaattgctga      300

taaagaagta aattctgttg tagatgtaat aggtattgcc aaaagtgtca gtgaattaca      360

aacattccaa gcaagatcaa caggaagaga attgaaaaag aaagaagttg tcttggttga      420

tcagtcacaa acagctatat cgttaacact ttggggccaa gaagccgaaa attttgatgg      480

taccaataat cctgtcgtag                                                  500


<210>  27
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  27
tcccgaatta aaagaatgtt acaggatcag aggatggtac gacagtgagg gtgaaaatct       60

gaatgcaaag aatattagtg ccagagttgg atcctcgaat atgtctgcca cttggatgac      120

ctttaaggaa gttaaagatc aaaaattagg atcatctgaa aaaggtgatt attataaagc      180

tattgctact gttcttcttg tcaaagccga taatattgtg tatagagctt gtcccaccgc      240

tgaatgtaat aagaaagttg ttgatatgga aaatagtatg tacagatgtg aaaaatgtaa      300

tagagaattt ccaaatttca aatacagact gttagccagc atgaatgttg gagaccacac      360

aggaaaccaa tgggttagca tgttcagttc agaagccgaa aaaattctgg ggatgactgc      420

tgaggaagta ggacagacct tggaacacaa taaagaagaa atagccaaca tcgtagatag      480

agctcatttt aaagtattta gtcttacttg cagggcaaaa attgagactt acaatgatga      540

agctcg                                                                 546


<210>  28
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  28
tcaatatgaa gttgaacgta tcgtacccgg ccacgggttg ccaaaaactt ttcgaagttg       60

ttgacgaaca caaaattcgt atcttttacg aaaaacgcat gggtcaagaa gttgaggctg      120

atgctcttgg tgacgaatgg aagggctaca tcttgaaaat atctggaggt aacgacaaac      180

aaggattccc catgaaacaa ggtgttctta caaacggtag agtaagactt ttactttcaa      240

aaggtcactc ctgctacaga cccagacgta ccggtgaacg taaaaggaaa tcagttcgtg      300

ggtgcattgt tgatgggaac ctcagcgtgt tggccctagt cattgtaaga aaaggagaac      360

aagaaattcc cggacttact gacaccacca tcccacgtcg cctgggaccc aagagagcat      420


<210>  29
<211>  817
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hairpin

<400>  29
cctctgaaga tttgcggtga tatacatggt cagtactatg acttgcttcg tctctttgaa       60

tatggaggtt tccctcccga atcaaactac ttatttttgg gagattatgt agatcgtggt      120

aaacaatcat tggaaaccat ctgcttactt ctcgcttaca aaattaaata cccagaaaac      180

tttttcctac tcagaggcaa ccacgaatgc gcatcaatta atcgtatata tggattctat      240

gatgaatgca aaagaaggta taacatcaag ttgtggaaaa cttttacgga ctgtttgact      300

agtaccggtt gggaaaggta tgtttctgct tctacctttg atatatatat aataattatc      360

actaattagt agtaatatag tatttcaagt atttttttca aaataaaaga atgtagtata      420

tagctattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata      480

tatgaccaaa acatggtgat gtgcaggttg atccgcggtt aaaacagtcc gtaaaagttt      540

tccacaactt gatgttatac cttcttttgc attcatcata gaatccatat atacgattaa      600

ttgatgcgca ttcgtggttg cctctgagta ggaaaaagtt ttctgggtat ttaattttgt      660

aagcgagaag taagcagatg gtttccaatg attgtttacc acgatctaca taatctccca      720

aaaataagta gtttgattcg ggagggaaac ctccatattc aaagagacga agcaagtcat      780

agtactgacc atgtatatca ccgcaaatct tcagagg                               817


<210>  30
<211>  807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hairpin

<400>  30
tacctgcagg tggataaagt atattacatc aacaaatgtc aacttaaaca agccaacaaa       60

cagtacagca ctctaaaaca tgagtatgaa atgactgtta cgcatgatac tgtcattaaa      120

gaatgccttg atgcagattc tacaataccc accacacagt ataactttgt tcctatagat      180

aaaattgctg ataaagaagt aaattctgtt gtagatgtaa taggtattgc caaaagtgtc      240

agtgaattac aaacattcca agcaagatca acaggaagag aattgaaaaa ggactagtac      300

cggttgggaa aggtatgttt ctgcttctac ctttgatata tatataataa ttatcactaa      360

ttagtagtaa tatagtattt caagtatttt tttcaaaata aaagaatgta gtatatagct      420

attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga      480

ccaaaacatg gtgatgtgca ggttgatccg cggttacttt ttcaattctc ttcctgttga      540

tcttgcttgg aatgtttgta attcactgac acttttggca atacctatta catctacaac      600

agaatttact tctttatcag caattttatc tataggaaca aagttatact gtgtggtggg      660

tattgtagaa tctgcatcaa ggcattcttt aatgacagta tcatgcgtaa cagtcatttc      720

atactcatgt tttagagtgc tgtactgttt gttggcttgt ttaagttgac atttgttgat      780

gtaatatact ttatccacct gcaggta                                          807


<210>  31
<211>  825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hairpin

<400>  31
aacgacaaac aaggattccc catgaaacaa ggtgttctta caaacggtag agtaagactt       60

ttactttcaa aaggtcactc ctgctacaga cccagacgta ccggtgaacg taaaaggaaa      120

tcagttcgtg ggtgcattgt tgatgggaac ctcagcgtgt tggccctagt cattgtaaga      180

aaaggagaac aagaaattcc cggacttact gacaccacca tcccacgtcg cctgggaccc      240

aagagagcat ccagaatccg taagctcttc aaccttagca aagaagacga tgtacgtcaa      300

gactagtacc ggttgggaaa ggtatgtttc tgcttctacc tttgatatat atataataat      360

tatcactaat tagtagtaat atagtatttc aagtattttt ttcaaaataa aagaatgtag      420

tatatagcta ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct      480

aatatatgac caaaacatgg tgatgtgcag gttgatccgc ggttattgac gtacatcgtc      540

ttctttgcta aggttgaaga gcttacggat tctggatgct ctcttgggtc ccaggcgacg      600

tgggatggtg gtgtcagtaa gtccgggaat ttcttgttct ccttttctta caatgactag      660

ggccaacacg ctgaggttcc catcaacaat gcacccacga actgatttcc ttttacgttc      720

accggtacgt ctgggtctgt agcaggagtg accttttgaa agtaaaagtc ttactctacc      780

gtttgtaaga acaccttgtt tcatggggaa tccttgtttg tcgtt                      825


<210>  32
<211>  218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  32
tagctctgat gacagagccc atcgagtttc aagccaaaca gttgcataaa gctatcagcg       60

gattgggaac tgatgaaagt acaatmgtmg aaattttaag tgtmcacaac aacgatgaga      120

ttataagaat ttcccaggcc tatgaaggat tgtaccaacg mtcattggaa tctgatatca      180

aaggagatac ctcaggaaca ttaaaaaaga attattag                              218


<210>  33
<211>  424
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment


<220>
<221>  misc_feature
<222>  (393)..(395)
<223>  n is a, c, g, or t

<400>  33
ttgttacaag ctggagaact tctctttgct ggaaccgaag agtcagtatt taatgctgta       60

ttctgtcaaa gaaataaacc acaattgaat ttgatattcg acaaatatga agaaattgtt      120

gggcatccca ttgaaaaagc cattgaaaac gagttttcag gaaatgctaa acaagccatg      180

ttacacctta tccagagcgt aagagatcaa gttgcatatt tggtaaccag gctgcatgat      240

tcaatggcag gcgtcggtac tgacgataga actttaatca gaattgttgt ttcgagatct      300

gaaatcgatc tagaggaaat caaacaatgc tatgaagaaa tctacagtaa aaccttggct      360

gataggatag cggatgacac atctggcgac tannnaaaag ccttattagc cgttgttggt      420

taag                                                                   424


<210>  34
<211>  397
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  34
agatgttggc tgcatctaga gaattacaca agttcttcca tgattgcaag gatgtactga       60

gcagaatagt ggaaaaacag gtatccatgt ctgatgaatt gggaagggac gcaggagctg      120

tcaatgccct tcaacgcaaa caccagaact tcctccaaga cctacaaaca ctccaatcga      180

acgtccaaca aatccaagaa gaatcagcta aacttcaagc tagctatgcc ggtgatagag      240

ctaaagaaat caccaacagg gagcaggaag tggtagcagc ctgggcagcc ttgcagatcg      300

cttgcgatca gagacacgga aaattgagcg atactggtga tctattcaaa ttctttaact      360

tggtacgaac gttgatgcag tggatggacg aatggac                               397


<210>  35
<211>  490
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  35
gcagatgaac accagcgaga aaccaagaga tgttagtggt gttgaattgt tgatgaacaa       60

ccatcagaca ctcaaggctg agatcgaagc cagagaagac aactttacgg cttgtatttc      120

tttaggaaag gaattgttga gccgtaatca ctatgctagt gctgatatta aggataaatt      180

ggtcgcgttg acgaatcaaa ggaatgctgt actacagagg tgggaagaaa gatgggagaa      240

cttgcaactc atcctcgagg tataccaatt cgccagagat gcggccgtcg ccgaagcatg      300

gttgatcgca caagaacctt acttgatgag ccaagaacta ggacacacca ttgacgacgt      360

tgaaaacttg ataaagaaac acgaagcgtt cgaaaaatcg gcagcggcgc aagaagagag      420

attcagtgct ttggagagac tgacgacgtt cgaattgaga gaaataaaga ggaaacaaga      480

agctgcccag                                                             490


<210>  36
<211>  330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  36
agtgaaatgt tagcaaatat aacatccaag tttcgtaatt gtacttgctc agttagaaaa       60

tattctgtag tttcactatc ttcaaccgaa aatagaataa atgtagaacc tcgcgaactt      120

gcctttcctc caaaatatca agaacctcga caagtttggt tggagagttt agatacgata      180

gacgacaaaa aattgggtat tcttgagctg catcctgatg tttttgctac taatccaaga      240

atagatatta tacatcaaaa tgttagatgg caaagtttat atagatatgt aagctatgct      300

catacaaagt caagatttga agtgagaggt                                       330


<210>  37
<211>  320
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  37
caaagtcaag atttgaagtg agaggtggag gtcgaaaacc gtggccgcaa aagggattgg       60

gacgtgctcg acatggttca attagaagtc cactttggag aggtggagga gttgttcatg      120

gaccaaaatc tccaacccct catttttaca tgattccatt ctacacccgt ttgctgggtt      180

tgactagcgc actttcagta aaatttgccc aagatgactt gcacgttgtg gatagtctag      240

atctgccaac tgacgaacaa agttatatag aagagctggt caaaagccgc ttttgggggt      300

ccttcttgtt ttatttgtag                                                  320


<210>  38
<211>  503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  38
caccatgggc tccagcggcg ccctgctgtt ccacggcaag atcccctacg tggtggagat       60

ggagggcaat gtggatggcc acaccttcag catccgcggc aagggctacg gcgatgccag      120

cgtgggcaag gtggatgccc agttcatctg caccaccggc gatgtgcccg tgccctggag      180

caccctggtg accaccctga cctacggcgc ccagtgcttc gccaagtacg gccccgagct      240

gaaggatttc tacaagagct gcatgcccga tggctacgtg caggagcgca ccatcacctt      300

cgagggcgat ggcaatttca agacccgcgc cgaggtgacc ttcgagaatg gcagcgtgta      360

caatcgcgtg aagctgaatg gccagggctt caagaaggat ggccacgtgc tgggcaagaa      420

tctggagttc aatttcaccc cccactgcct gtacatctgg ggcgatcagg ccaatcacgg      480

cctgaagagc gccttcaaga tct                                              503


<210>  39
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  39
ttaatacgac tcactatagg gagagctcca acagtggttc cttatc                      46


<210>  40
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  40
ctaataattc ttttttaatg ttcctgagg                                         29


<210>  41
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  41
gctccaacag tggttcctta tc                                                22


<210>  42
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  42
ttaatacgac tcactatagg gagactaata attctttttt aatgttcctg agg              53


<210>  43
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  43
ttaatacgac tcactatagg gagattgtta caagctggag aacttctc                    48


<210>  44
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  44
cttaaccaac aacggctaat aagg                                              24


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  45
ttgttacaag ctggagaact tctc                                              24


<210>  46
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  46
ttaatacgac tcactatagg gagacttaac caacaacggc taataagg                    48


<210>  47
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  47
ttaatacgac tcactatagg gagaagatgt tggctgcatc tagagaa                     47


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  48
gtccattcgt ccatccactg ca                                                22


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  49
agatgttggc tgcatctaga gaa                                               23


<210>  50
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  50
ttaatacgac tcactatagg gagagtccat tcgtccatcc actgca                      46


<210>  51
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  51
ttaatacgac tcactatagg gagagcagat gaacaccagc gagaaa                      46


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  52
ctgggcagct tcttgtttcc tc                                                22


<210>  53
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  53
gcagatgaac accagcgaga aa                                                22


<210>  54
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  54
ttaatacgac tcactatagg gagactgggc agcttcttgt ttcctc                      46


<210>  55
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  55
ttaatacgac tcactatagg gagaagtgaa atgttagcaa atataacatc c                51


<210>  56
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  56
acctctcact tcaaatcttg actttg                                            26


<210>  57
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  57
agtgaaatgt tagcaaatat aacatcc                                           27


<210>  58
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  58
ttaatacgac tcactatagg gagaacctct cacttcaaat cttgactttg                  50


<210>  59
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  59
ttaatacgac tcactatagg gagacaaagt caagatttga agtgagaggt                  50


<210>  60
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  60
ctacaaataa aacaagaagg acccc                                             25


<210>  61
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  61
caaagtcaag atttgaagtg agaggt                                            26


<210>  62
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  62
ttaatacgac tcactatagg gagactacaa ataaaacaag aaggacccc                   49


<210>  63
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  63
ttaatacgac tcactatagg gagacaccat gggctccagc ggcgccc                     47


<210>  64
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  64
agatcttgaa ggcgctcttc agg                                               23


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  65
caccatgggc tccagcggcg ccc                                               23


<210>  66
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  66
ttaatacgac tcactatagg gagaagatct tgaaggcgct cttcagg                     47


<210>  67
<211>  327
<212>  PRT
<213>  Diabrotica virgifera

<400>  67

Met Ala Glu Ala Asp Lys Leu Asn Ile Asp Ser Ile Ile Ala Arg Leu 
1               5                   10                  15      


Leu Glu Val Arg Gly Ala Arg Pro Gly Lys Asn Val Gln Leu Thr Glu 
            20                  25                  30          


Asn Glu Ile Arg Gly Leu Cys Leu Lys Ser Arg Glu Ile Phe Leu Ser 
        35                  40                  45              


Gln Pro Ile Leu Leu Glu Leu Glu Ala Pro Leu Lys Ile Cys Gly Asp 
    50                  55                  60                  


Ile His Gly Gln Tyr Tyr Asp Leu Leu Arg Leu Phe Glu Tyr Gly Gly 
65                  70                  75                  80  


Phe Pro Pro Glu Ser Asn Tyr Leu Phe Leu Gly Asp Tyr Val Asp Arg 
                85                  90                  95      


Gly Lys Gln Ser Leu Glu Thr Ile Cys Leu Leu Leu Ala Tyr Lys Ile 
            100                 105                 110         


Lys Tyr Pro Glu Asn Phe Phe Leu Leu Arg Gly Asn His Glu Cys Ala 
        115                 120                 125             


Ser Ile Asn Arg Ile Tyr Gly Phe Tyr Asp Glu Cys Lys Arg Arg Tyr 
    130                 135                 140                 


Asn Ile Lys Leu Trp Lys Thr Phe Thr Asp Cys Phe Asn Cys Leu Pro 
145                 150                 155                 160 


Val Ala Ala Ile Val Asp Glu Lys Ile Phe Cys Cys His Gly Gly Leu 
                165                 170                 175     


Ser Pro Asp Leu Gln Ser Met Glu Gln Ile Arg Arg Ile Met Arg Pro 
            180                 185                 190         


Thr Asp Val Pro Asp Gln Gly Leu Leu Cys Asp Leu Leu Trp Ser Asp 
        195                 200                 205             


Pro Asp Lys Asp Gln Met Gly Trp Gly Glu Asn Asp Arg Gly Val Ser 
    210                 215                 220                 


Phe Thr Phe Gly Ala Glu Val Val Gly Lys Phe Leu His Lys His Asp 
225                 230                 235                 240 


Phe Asp Leu Ile Cys Arg Ala His Gln Val Val Glu Asp Gly Tyr Glu 
                245                 250                 255     


Phe Phe Ala Lys Arg Gln Leu Val Thr Leu Phe Ser Ala Pro Asn Tyr 
            260                 265                 270         


Cys Gly Glu Phe Asp Asn Ala Gly Ala Met Met Ser Val Asp Glu Thr 
        275                 280                 285             


Leu Met Cys Ser Phe Gln Ile Leu Lys Pro Ala Asp Lys Arg Lys Phe 
    290                 295                 300                 


Gln Tyr Asn Met Asn Ala Gly Arg Pro Val Thr Pro Pro Arg Gly Ala 
305                 310                 315                 320 


Thr Asn Lys Asn Lys Lys Lys 
                325         


<210>  68
<211>  613
<212>  PRT
<213>  Diabrotica virgifera

<400>  68

Met Arg Ser Pro Gln Thr Tyr Asn Met Ser Glu Gly Ser Leu Gln Thr 
1               5                   10                  15      


Ile Met Ser Gly Gly Glu Phe Pro Asn Pro Ile Met Gln Val Leu Gly 
            20                  25                  30          


Ser Lys Lys Ile Asn Ala Gly Leu Gly Asp Lys Glu Arg Ile Arg Ile 
        35                  40                  45              


Leu Leu Ser Asp Gly Lys Tyr Thr Ile Ser Phe Ala Met Leu Thr Ala 
    50                  55                  60                  


Gln Ile Asn Asp Arg Leu Gly Pro Asn Gly Val Glu Thr Phe Ser Ile 
65                  70                  75                  80  


Ile Gln Ile Asp Arg Tyr Val Thr Ser Ile Ile Asn Asn Ser Gly Lys 
                85                  90                  95      


Gly Glu Ala Arg Val Leu Leu Ile Leu Asp Met His Val Val Val Pro 
            100                 105                 110         


Gly Thr Glu Val Thr Glu Lys Val Gly Ser Pro Ile Pro Leu Pro Thr 
        115                 120                 125             


Asp Ala Asp Ala Ala Lys Gly Ser Thr Ala Ala Pro Ala Thr Asn Asn 
    130                 135                 140                 


Ser Ile Lys Asn Val Thr Val Ala Lys Pro Asn Ile Ser Asn Gly Asn 
145                 150                 155                 160 


Gly Thr Thr Ala Met Asn Ala Ser Thr Asn Asp Asp Ile Ala Thr His 
                165                 170                 175     


Met Ile His Pro Ile Ser Ser Leu Thr Pro Tyr Gln Asn Arg Trp Thr 
            180                 185                 190         


Ile Lys Ala Arg Ile Thr Asn Lys Pro Pro Ile Arg Thr Trp Ser Asn 
        195                 200                 205             


Ser Arg Gly Glu Gly Lys Leu Phe Ser Phe Asp Leu Val Asp Glu Ser 
    210                 215                 220                 


Gly Glu Ile Arg Cys Thr Ala Phe Lys Glu Met Val Asp Lys Phe Tyr 
225                 230                 235                 240 


Asp Tyr Leu Gln Val Asp Lys Val Tyr Tyr Ile Asn Lys Cys Gln Leu 
                245                 250                 255     


Lys Gln Ala Asn Lys Gln Tyr Ser Thr Leu Lys His Glu Tyr Glu Met 
            260                 265                 270         


Thr Val Thr His Asp Thr Val Ile Lys Glu Cys Leu Asp Ala Asp Ser 
        275                 280                 285             


Thr Ile Pro Thr Thr Gln Tyr Asn Phe Val Pro Ile Asp Lys Ile Ala 
    290                 295                 300                 


Asp Lys Glu Val Asn Ser Val Val Asp Val Ile Gly Ile Ala Lys Ser 
305                 310                 315                 320 


Val Ser Glu Leu Gln Thr Phe Gln Ala Arg Ser Thr Gly Arg Glu Leu 
                325                 330                 335     


Lys Lys Lys Glu Val Val Leu Val Asp Gln Ser Gln Thr Ala Ile Ser 
            340                 345                 350         


Leu Thr Leu Trp Gly Gln Glu Ala Glu Asn Phe Asp Gly Thr Asn Asn 
        355                 360                 365             


Pro Val Val Val Ile Lys Ser Ala Lys Ile Gly Glu Phe Gly Gly Gly 
    370                 375                 380                 


Lys Asn Leu Thr Thr Leu Val Ser Ser Thr Val Lys Ile Asn Pro Glu 
385                 390                 395                 400 


Leu Lys Glu Cys Tyr Arg Ile Arg Gly Trp Tyr Asp Ser Glu Gly Glu 
                405                 410                 415     


Asn Leu Asn Ala Lys Asn Ile Ser Ala Arg Val Gly Ser Ser Asn Met 
            420                 425                 430         


Ser Ala Thr Trp Met Thr Phe Lys Glu Val Lys Asp Gln Lys Leu Gly 
        435                 440                 445             


Ser Ser Glu Lys Gly Asp Tyr Tyr Lys Ala Ile Ala Thr Val Leu Leu 
    450                 455                 460                 


Val Lys Ala Asp Asn Ile Val Tyr Arg Ala Cys Pro Thr Ala Glu Cys 
465                 470                 475                 480 


Asn Lys Lys Val Val Asp Met Glu Asn Ser Met Tyr Arg Cys Glu Lys 
                485                 490                 495     


Cys Asn Arg Glu Phe Pro Asn Phe Lys Tyr Arg Leu Leu Ala Ser Met 
            500                 505                 510         


Asn Val Gly Asp His Thr Gly Asn Gln Trp Val Ser Met Phe Ser Ser 
        515                 520                 525             


Glu Ala Glu Lys Ile Leu Gly Met Thr Ala Glu Glu Val Gly Gln Thr 
    530                 535                 540                 


Leu Glu His Asn Lys Glu Glu Ile Ala Asn Ile Val Asp Arg Ala His 
545                 550                 555                 560 


Phe Lys Val Phe Ser Leu Thr Cys Arg Ala Lys Ile Glu Thr Tyr Asn 
                565                 570                 575     


Asp Glu Ala Arg Leu Lys Thr Val Cys Ile Arg Val Asp Pro Ile Asn 
            580                 585                 590         


Tyr Glu Glu Tyr Ser Ala Leu Leu Thr Glu Lys Ile Gln Gln Leu Thr 
        595                 600                 605             


Gly Glu Ser His Asp 
    610             


<210>  69
<211>  248
<212>  PRT
<213>  Diabrotica virgifera

<400>  69

Met Lys Leu Asn Val Ser Tyr Pro Ala Thr Gly Cys Gln Lys Leu Phe 
1               5                   10                  15      


Glu Val Val Asp Glu His Lys Ile Arg Ile Phe Tyr Glu Lys Arg Met 
            20                  25                  30          


Gly Gln Glu Val Glu Ala Asp Ala Leu Gly Asp Glu Trp Lys Gly Tyr 
        35                  40                  45              


Ile Leu Lys Ile Ser Gly Gly Asn Asp Lys Gln Gly Phe Pro Met Lys 
    50                  55                  60                  


Gln Gly Val Leu Thr Asn Gly Arg Val Arg Leu Leu Leu Ser Lys Gly 
65                  70                  75                  80  


His Ser Cys Tyr Arg Pro Arg Arg Thr Gly Glu Arg Lys Arg Lys Ser 
                85                  90                  95      


Val Arg Gly Cys Ile Val Asp Gly Asn Leu Ser Val Leu Ala Leu Val 
            100                 105                 110         


Ile Val Arg Lys Gly Glu Gln Glu Ile Pro Gly Leu Thr Asp Thr Thr 
        115                 120                 125             


Ile Pro Arg Arg Leu Gly Pro Lys Arg Ala Ser Arg Ile Arg Lys Leu 
    130                 135                 140                 


Phe Asn Leu Ser Lys Glu Asp Asp Val Arg Gln Tyr Val Val Lys Arg 
145                 150                 155                 160 


Pro Leu Ala Gln Lys Glu Gly Lys Lys Leu Arg Thr Lys Ala Pro Lys 
                165                 170                 175     


Ile Gln Arg Leu Ile Thr Pro Val Val Leu Gln Arg Lys Arg His Arg 
            180                 185                 190         


Leu Ala Leu Lys Lys Lys Arg Cys Leu Lys Arg Lys Glu Gln Glu Asp 
        195                 200                 205             


Ala Tyr Ala Lys Leu Leu Ala Gln Arg Lys Lys Glu Ser Lys Ala Arg 
    210                 215                 220                 


Arg Glu Met Leu Lys Arg Arg Arg Ser Ala Ser Met Arg Asp Ser Lys 
225                 230                 235                 240 


Ser Ser Thr Gln Ser Gly Gln Lys 
                245             


<210>  70
<211>  1150
<212>  DNA
<213>  Zea mays

<400>  70
caacggggca gcactgcact gcactgcaac tgcgaatttc cgtcagcttg gagcggtcca       60

agcgccctgc gaagcaaact acgccgatgg cttcggcggc ggcgtgggag ggtccgacgg      120

ccgcggagct gaagacagcg ggggcggagg tgattcccgg cggcgtgcga gtgaaggggt      180

gggtcatcca gtcccacaaa ggccctatcc tcaacgccgc ctctctgcaa cgctttgaag      240

atgaacttca aacaacacat ttacctgaga tggtttttgg agagagtttc ttgtcacttc      300

aacatacaca aactggcatc aaatttcatt ttaatgcgct tgatgcactc aaggcatgga      360

agaaagaggc actgccacct gttgaggttc ctgctgcagc aaaatggaag ttcagaagta      420

agccttctga ccaggttata cttgactacg actatacatt tacgacacca tattgtggga      480

gtgatgctgt ggttgtgaac tctggcactc cacaaacaag tttagatgga tgcggcactt      540

tgtgttggga ggatactaat gatcggattg acattgttgc cctttcagca aaagaaccca      600

ttcttttcta cgacgaggtt atcttgtatg aagatgagtt agctgacaat ggtatctcat      660

ttcttactgt gcgagtgagg gtaatgccaa ctggttggtt tctgcttttg cgtttttggc      720

ttagagttga tggtgtactg atgaggttga gagacactcg gttacattgc ctgtttggaa      780

acggcgacgg agccaagcca gtggtacttc gtgagtgctg ctggagggaa gcaacatttg      840

ctactttgtc tgcgaaagga tatccttcgg actctgcagc gtacgcggac ccgaacctta      900

ttgcccataa gcttcctatt gtgacgcaga agacccaaaa gctgaaaaat cctacctgac      960

tgacacaaag gcgccctacc gcgtgtacat catgactgtc ctgtcctatc gttgcctttt     1020

gtgtttgcca catgttgtgg atgtacgttt ctatgacgaa acaccatagt ccatttcgcc     1080

tgggccgaac agagatagct gattgtcatg tcacgtttga attagaccat tccttagccc     1140

tttttccccc                                                            1150


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  71
agccaagcca gtggtacttc                                                   20


<210>  72
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  72
tcgcagacaa agtagcaaat gt                                                22


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  73
ttgtgatgtt ggtggcgtat                                                   20


<210>  74
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  74
tgttaaataa aaccccaaag atcg                                              24


<210>  75
<211>  2013
<212>  DNA
<213>  Zea mays

<400>  75
atgatccctg ccgttgctga tccgacgacg ctggacggcg ggggcgcgcg caggccgttg       60

ctcccggaga cggaccctcg ggggcgtgct gccgccggcg ccgagcagaa gcggccgccg      120

gctacgccga ccgttctcac cgccgtcgtc tccgccgtgc tcctgctcgt cctcgtggcg      180

gtcacagtcc tcgcgtcgca gcacgtcgac gggcaggctg ggggcgttcc cgcgggcgaa      240

gatgccgtcg tcgtcgaggt ggccgcctcc cgtggcgtgg ctgagggcgt gtcggagaag      300

tccacggccc cgctcctcgg ctccggcgcg ctccaggact tctcctggac caacgcgatg      360

ctggcgtggc agcgcacggc gttccacttc cagcccccca agaactggat gaacgatccg      420

aacggtccgc tgtatcacaa gggctggtac cacctcttct accagtggaa cccggactcc      480

gcggtatggg gcaacatcac ctggggccac gccgtctcgc gcgacctcct ccactggctg      540

cacctaccgc tggccatggt gcccgatcac ccgtacgacg ccaacggcgt ctggtccggg      600

tcggcgacgc gcctgcccga cggccggatc gtcatgctct acacgggctc cacggcggag      660

tcgtcggcgc aggtgcagaa cctcgcggag ccggccgacg cgtccgaccc gctgctgcgg      720

gagtgggtca agtcggacgc caacccggtg ctggtgccgc cgccgggcat cgggccgacg      780

gacttccgcg acccgacgac ggcgtgtcgg acgccggccg gcaacgacac ggcgtggcgg      840

gtcgccatcg ggtccaagga ccgggaccac gcggggctgg cgctggtgta ccggacggag      900

gacttcgtgc ggtacgaccc ggcgccggcg ctgatgcacg ccgtgccggg caccggcatg      960

tgggagtgcg tggacttcta cccggtggcc gcgggatcag gcgccgcggc gggcagcggg     1020

gacgggctgg agacgtccgc ggcgccggga cccggggtga agcacgtgct caaggctagc     1080

ctcgacgacg acaagcacga ctactacgcg atcggcacct acgacccggc gacggacacc     1140

tggacccccg acagcgcgga ggacgacgtc gggatcggcc tccggtacga ctatggcaag     1200

tactacgcgt cgaagacctt ctacgacccc gtccttcgcc ggcgggtgct ctgggggtgg     1260

gtcggcgaga ccgacagcga gcgcgcggac atcctcaagg gctgggcatc cgtgcagtca     1320

atccccagga cggtcctcct ggacacgaag acgggcagca acctgctcca gtggccggtg     1380

gtggaggtgg agaacctccg gatgagcggc aagagcttcg acggcgtcgc gctggaccgc     1440

ggatccgtcg tgcccctcga cgtcggcaag gcgacgcagt tggacatcga ggctgtgttc     1500

gaggtggacg cgtcggacgc ggcgggcgtc acggaggccg acgtgacgtt caactgcagc     1560

accagcgcag gcgcggcggg ccggggcctg ctcggcccgt tcggccttct cgtgctggcg     1620

gacgacgact tgtccgagca gaccgccgtg tacttctacc tgctcaaggg cacggacggc     1680

agcctccaaa ctttcttctg ccaagacgag ctcagggcat ccaaggcgaa cgatctggtt     1740

aagagagtat acgggagctt ggtccctgtg ctagatgggg agaatctctc ggtcagaata     1800

ctggttgacc actccatcgt ggagagcttt gctcaaggcg ggaggacgtg catcacgtcg     1860

cgagtgtacc ccacacgagc catctacgac tccgcccgcg tcttcctctt caacaacgcc     1920

acacatgctc acgtcaaagc aaaatccgtc aagatctggc agctcaactc cgcctacatc     1980

cggccatatc cggcaacgac gacttctcta tga                                  2013


<210>  76
<211>  786
<212>  DNA
<213>  Escherichia coli

<400>  76
atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc       60

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      120

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      180

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      240

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      300

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      360

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      420

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      480

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      540

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      600

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      660

cagcccgtca tacttgaagc taggcaggct tatcttggac aagaagatcg cttggcctcg      720

cgcgcagatc agttggaaga atttgttcac tacgtgaaag gcgagatcac caaggtagtc      780

ggcaaa                                                                 786


<210>  77
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  77
gtatgtttct gcttctacct ttgatatata tataataatt atcactaatt agtagtaata       60

tagtatttca agtatttttt tcaaaataaa agaatgtagt atatagctat tgcttttctg      120

tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaacatggt      180

gatgtgcag                                                              189


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  78
tgttcggttc cctctaccaa                                                   20


<210>  79
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  79
caacatccat caccttgact ga                                                22


<210>  80
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe Oligonucleotide

<400>  80
cacagaaccg tcgcttcagc aaca                                              24


<210>  81
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  81
tggcggacga cgacttgt                                                     18


<210>  82
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  82
aaagtttgga ggctgccgt                                                    19


<210>  83
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe Oligonucleotide

<400>  83
cgagcagacc gccgtgtact tctacc                                            26


<210>  84
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  84
gtatgtttct gcttctacct ttgat                                             25


<210>  85
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  85
ccatgttttg gtcatatatt agaaaagtt                                         29


<210>  86
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe Oligonucleotide

<400>  86
agtaatatag tatttcaagt atttttttca aaat                                   34


<210>  87
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  87
cttagctgga taacgccac                                                    19


<210>  88
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  88
gaccgtaagg cttgatgaa                                                    19


<210>  89
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe Oligonucleotide

<400>  89
cgagattctc cgcgctgtag a                                                 21


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  90
gaatccttgc gtcatttggt                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  91
caatggactc acgcacaact                                                   20


<210>  92
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  92
gaatccttgc gtcatttggt gactagtacc ggttggga                               38


<210>  93
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  93
caatggactc acgcacaact taaccgcgga tcaa                                   34


<210>  94
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe Oligonucleotide

<400>  94
tgggaaaggt tg                                                           12


<210>  95
<211>  213
<212>  PRT
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (200)..(200)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (206)..(206)
<223>  Xaa can be any naturally occurring amino acid

<400>  95

Met Ala Glu Ala Asp Lys Leu Asn Ile Asp Ser Ile Ile Ala Arg Leu 
1               5                   10                  15      


Leu Glu Val Arg Gly Ala Arg Pro Gly Lys Asn Val Gln Leu Thr Glu 
            20                  25                  30          


Asn Glu Ile Arg Gly Leu Cys Leu Lys Ser Arg Glu Ile Phe Leu Ser 
        35                  40                  45              


Gln Pro Ile Leu Leu Glu Leu Glu Ala Pro Leu Lys Ile Cys Gly Asp 
    50                  55                  60                  


Ile His Gly Gln Tyr Tyr Asp Leu Leu Arg Leu Phe Glu Tyr Gly Gly 
65                  70                  75                  80  


Phe Pro Pro Glu Ser Asn Tyr Leu Phe Leu Gly Asp Tyr Val Asp Arg 
                85                  90                  95      


Gly Lys Gln Ser Leu Glu Thr Ile Cys Leu Leu Leu Ala Tyr Lys Ile 
            100                 105                 110         


Lys Tyr Pro Glu Asn Phe Phe Leu Leu Arg Gly Asn His Glu Cys Ala 
        115                 120                 125             


Ser Ile Asn Arg Ile Tyr Gly Phe Tyr Asp Glu Cys Lys Arg Arg Tyr 
    130                 135                 140                 


Asn Ile Lys Leu Trp Lys Thr Phe Thr Asp Cys Phe Asn Cys Leu Pro 
145                 150                 155                 160 


Val Ala Ala Ile Val Asp Glu Lys Ile Phe Cys Cys His Gly Gly Leu 
                165                 170                 175     


Ser Pro Asp Leu Gln Ser Met Glu Gln Ile Arg Arg Ile Asn Arg Asp 
            180                 185                 190         


Arg Leu Met Tyr Leu Thr Lys Xaa Phe Phe Val Thr Phe Xaa Gly Leu 
        195                 200                 205             


Ile Gln Thr Lys Thr 
    210             


<210>  96
<211>  120
<212>  PRT
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Xaa can be any naturally occurring amino acid

<400>  96

Met Ile Asp Leu Val Gln Met Val Trp Lys Leu Phe Ser Ile Ile Gln 
1               5                   10                  15      


Ile Asp Arg Tyr Val Thr Ser Ile Ile Asn Asn Ser Gly Lys Gly Glu 
            20                  25                  30          


Ala Arg Val Leu Leu Ile Leu Asp Met His Val Val Val Pro Gly Thr 
        35                  40                  45              


Glu Val Thr Glu Lys Val Gly Ser Pro Ile Pro Leu Pro Thr Asp Ala 
    50                  55                  60                  


Asp Xaa Ala Lys Gly Ser Thr Ala Ala Pro Ala Thr Asn Asn Ser Ile 
65                  70                  75                  80  


Lys Asn Val Thr Val Ala Lys Pro Asn Ile Ser Asn Gly Asn Gly Thr 
                85                  90                  95      


Thr Ala Met Asn Ala Ser Thr Asn Asp Asp Ile Ala Thr His Met Ile 
            100                 105                 110         


His Pro Ile Ser Ser Leu Thr Pro 
        115                 120 


<210>  97
<211>  169
<212>  PRT
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa can be any naturally occurring amino acid

<400>  97

Trp Ser Asn Ser Arg Gly Glu Gly Lys Leu Phe Ser Phe Asp Leu Val 
1               5                   10                  15      


Asp Glu Ser Gly Glu Ile Arg Cys Thr Ala Phe Lys Glu Met Val Asp 
            20                  25                  30          


Xaa Phe Tyr Asp Tyr Leu Gln Val Asp Lys Val Tyr Tyr Ile Asn Lys 
        35                  40                  45              


Cys Gln Leu Lys Gln Ala Asn Lys Gln Tyr Ser Thr Leu Lys His Glu 
    50                  55                  60                  


Tyr Glu Met Thr Val Thr His Asp Thr Val Ile Lys Glu Cys Leu Asp 
65                  70                  75                  80  


Ala Asp Ser Thr Ile Pro Thr Thr Gln Tyr Asn Phe Val Pro Ile Asp 
                85                  90                  95      


Lys Ile Ala Asp Lys Glu Val Asn Ser Val Val Asp Val Ile Gly Ile 
            100                 105                 110         


Ala Lys Ser Val Ser Glu Leu Gln Thr Phe Gln Ala Arg Ser Thr Gly 
        115                 120                 125             


Arg Glu Leu Lys Lys Lys Glu Val Val Leu Val Asp Gln Ser Gln Thr 
    130                 135                 140                 


Ala Ile Ser Leu Thr Leu Trp Gly Gln Glu Ala Glu Asn Phe Asp Gly 
145                 150                 155                 160 


Thr Asn Asn Pro Val Val Val Ile Lys 
                165                 


<210>  98
<211>  225
<212>  PRT
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  98

Thr Leu Val Ser Ser Xaa Xaa Lys Ile Asn Pro Glu Leu Lys Glu Cys 
1               5                   10                  15      


Tyr Arg Ile Arg Gly Trp Tyr Asp Ser Glu Gly Glu Asn Leu Asn Ala 
            20                  25                  30          


Lys Asn Ile Ser Ala Arg Val Gly Ser Ser Asn Met Ser Ala Thr Trp 
        35                  40                  45              


Met Thr Phe Lys Glu Val Lys Asp Gln Lys Leu Gly Ser Ser Glu Lys 
    50                  55                  60                  


Gly Asp Tyr Tyr Lys Ala Ile Ala Thr Val Leu Leu Val Lys Ala Asp 
65                  70                  75                  80  


Asn Ile Val Tyr Arg Ala Cys Pro Thr Ala Glu Cys Asn Lys Lys Val 
                85                  90                  95      


Val Asp Met Glu Asn Ser Met Tyr Arg Cys Glu Lys Cys Asn Arg Glu 
            100                 105                 110         


Phe Pro Asn Phe Lys Tyr Arg Leu Leu Ala Ser Met Asn Val Gly Asp 
        115                 120                 125             


His Thr Gly Asn Gln Trp Val Ser Met Phe Ser Ser Glu Ala Glu Lys 
    130                 135                 140                 


Ile Leu Gly Met Thr Ala Glu Glu Val Gly Gln Thr Leu Glu His Asn 
145                 150                 155                 160 


Lys Glu Glu Ile Ala Asn Ile Val Asp Arg Ala His Phe Lys Val Phe 
                165                 170                 175     


Ser Leu Thr Cys Arg Ala Lys Ile Glu Thr Tyr Asn Asp Glu Ala Arg 
            180                 185                 190         


Leu Lys Thr Val Cys Ile Arg Val Asp Pro Ile Asn Tyr Glu Glu Tyr 
        195                 200                 205             


Ser Ala Leu Leu Thr Glu Lys Ile Gln Gln Leu Thr Gly Glu Ser His 
    210                 215                 220                 


Asp 
225 


<210>  99
<211>  17
<212>  DNA
<213>  Diabrotica virgifera

<400>  99
aattcaagct gccgcaa                                                      17


<210>  100
<211>  134
<212>  DNA
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (113)..(113)
<223>  n is a, c, g, or t

<400>  100
ttttctgttg ccatggtggt ttaagtccgg acctacaatc aatggaacaa attagaagra       60

ttaatagaga ccgactgatg tacctgacca aggstttctt tgtgaccttt tanggtctga      120

tccagacaaa gacc                                                        134


<210>  101
<211>  225
<212>  DNA
<213>  Diabrotica virgifera

<400>  101
tggaactgaa gttacagaaa aagtaggctc tcccattccc ctaccaactg atgctgackc       60

agctaaaggc tctactgccg ctccagctac aaacaattcc attaagaatg taactgttgc      120

taaaccaaac atcagtaatg gcaatggcac aactgcaatg aatgccagta ctaatgatga      180

tatagccaca catatgatcc atcctatttc aagtctcaca cctta                      225


<210>  102
<211>  244
<212>  DNA
<213>  Diabrotica virgifera

<400>  102
aactcttgtt agcagcamtr taaaaataaa tcccgaatta aaagaatgtt acaggatcag       60

aggatggtac gacagtgagg gtgaaaatct gaatgcaaag aatattagtg ccagagttgg      120

atcctcgaat atgtctgcca cttggatgac ctttaaggaa gttaaagatc aaaaattagg      180

atcatctgaa aaaggtgatt attataaagc tattgctact gttcttcttg tcaaagccga      240

taat                                                                   244


<210>  103
<211>  5134
<212>  DNA
<213>  Diabrotica virgifera

<400>  103
acagttaaat attgaaaatg gcctggtgtt ttgataaaac ggaagaggcg aatttctagt       60

agcattttaa ggtttcattt gcatttaaaa caaattcatg tattataaaa tgtaggatac      120

gtttcctcgt atccatctac ttaatttagg ataacaataa agggtgtgag acagttaaat      180

attgaaaatg gccagtgctt cattattacc caaaactttc acttctattg gtggcaaagc      240

cctacctacc aactcacaac aaaacattca gtcaaaattt aaagagatta cagttccacc      300

aggaaatact cctcaagatg ttaaagaagg ccccagtcac caatcaaatc caaaccattt      360

ggcttctctt caaaaggcca ttgaaactat ggaagagaag ggcttacaag ctgatcctag      420

atattcacag ttacttgcat tgcgagctag cattcctggg gcagaagaaa atggttctcc      480

cttctcaaac aaccaaatca agcaattaag aaaccaaata atggcttaca ggtgtttggc      540

aagaaatcaa cctgttccaa acaatttagt attaggtttg catggaaaaa ctcctgaaaa      600

agttccacat attgtacctc caccgcaacc tcaagaagta cctaatgggg gcgatccagg      660

accttcaaca agttctgctg ctgctgtagc tcctagaaca ccacaaaagc tgccagcaaa      720

accaattgag gctcagcttg tcaacagaga accaagagtc actactttat ctaaaccatc      780

ttccatagac cctgttgttc tattacaaga acgagaaaac agggtagcag ctcgtatagc      840

agcgaggatt gaacaagtca gtaatctgcc gactgatatg tctgaggcat tacgtattcg      900

ggcacaaata gaactcagag ctttgagatg tctaaacctc cagagacaac ttcgtagtga      960

ggttttgagc tgtattcgac gggacacaac attagaaaca gcagtaaatg taaaagcgtt     1020

taaacggacc aaacgtcaag gtcttcgaga agctagagca acagaaaaac ttgagaaaca     1080

acaaaagctg gaagcagaga gaaagaaacg ccagaagaac caagagttct taaacaatgt     1140

gatggcacac gctaaagatt tcaaagaatt ccacaggcag aaccaagcaa aactttctaa     1200

acttaataaa gctattctta cttatcacgc taatgcggag agagaacaaa agaaggaaca     1260

agagagaaga gaaaaggaac gtatgaagaa attgatggca gaagatgaag aaggttatag     1320

acagttgatc gatcaaaaga aagacaaacg tctagcgttc ttgctttcgc aaacagatga     1380

gtatataact aacctcacgg agatggtaaa gcaacacaag ttggaacaaa ccaataaaaa     1440

gaaagaggag gaaaaacgca agaagaagca gcagaaaatg caacaaccag ataggaaagt     1500

tacagttctg gaaactgcaa caggtaaaaa agtaacagga gaggctgctc ctacactgcg     1560

acaagttcag gaatggttaa tccaacatcc tggatgggag atggtcgata cagatgatga     1620

ggatgatgaa aacggggaga agagggatga tgactatgat gaaaatcaag aagtggatga     1680

tgcaaaagaa gttattaaaa aagctaaagt tgaagatgac gaatatcaca aaaacacaaa     1740

agaagaacag acttactaca gtattgctca cactgttcat gaagtggtaa cagaacaagc     1800

atccattctg gttaatggaa agcttaagga atatcaaatt agagggttag aatggatggt     1860

gtctttgtac aataacaatc tgaatggtat tctagcagat gagatgggtc taggtaaaac     1920

cattcaaacg attggcttgt tgacctattt gatggaaaaa aagaagataa atggaccgtt     1980

tttgatcata gtgccacttt caaccatttc taattggatg ttggaatttc aaaagtgggc     2040

ccctactgta gttgtcattt catacaaagg ctctcctgtg gttagaaaag tgatccagag     2100

ccagttaaaa gctgctaaat tcaatgtgct tctcactacc tacgagtaca ttattaagga     2160

caagggtgta ttagcaaaaa tcccatttaa atatatgatc atagatgagg gtcatcgtat     2220

gaaaaaccac cactgcaaat tgactcaagt cctgaatacg cactatttgg cgccctacag     2280

actcctgctt actggtactc ccctacaaaa taaattacca gaattatggg ccttgttgaa     2340

tttcttgttg ccttcgattt tcaagagttg ctccactttt gaacaatggt tcaatgcgcc     2400

attcgcaaca acaggagaaa aggttgagtt aaacgaagaa gaaactatcc ttatcatccg     2460

tcgtcttcac aaagtactca ggccgtttct cctgagacgt ctcaagaaag aagtcgaatc     2520

tcagcttcca gacaaagtgg aatatatcat aaagtgtgac atgtcgggcc tacaaaaggt     2580

tctctatgca cacatgcaga gcaagggtgt gttacttacc gatggttccg agaagggcag     2640

taaaggaagg ggatctaagg cactgatgaa caccattatg cagctgagga aactgtgcaa     2700

tcatccgttt atgttccaaa atatcgaaga gaaatattgt gatcatgttg gtattgctgg     2760

tggagtggtt tctggacccg acacttatag ggtatctggt aagtttgagc tcttggacag     2820

aatattgccc aaaatgaaag caactaacca taggattctt cttttctgtc aaatgactca     2880

attaatgacc atcatggaag attatctaaa ttggagagga ttcaaatatc ttcgtcttga     2940

tggtacaatc aaatcagaag atcgcgggga cctattatcg aaatttaatg ataaaaatag     3000

tgaatatttt ttgtttttgc tatctacacg ggctggaggt ctgggactta atttgcagac     3060

agctgatact gtgattatct tcgattccga ttggaatcct catcaggatt tacaagctca     3120

ggatcgagct catcgtattg gacagcaaaa tgaggtccga gttttgcgtt tgatgactgt     3180

taactctgtt gaggaacgaa ttttagctgc agctaaatac aagcttacta tggacgaaaa     3240

ggtcattcaa gctggtatgt tcgatcagaa gtctacaggc tcagagagac atcagttttt     3300

gcagagtatt ttacaccatg acggaagcga cgaagaagag gaaaacgaag ttcctgatga     3360

cgaaacagtg aaccagatgt tggcccgaag ggaaaacgaa tttcagcttt tccagaagat     3420

ggatcaggaa agaaaggaag aagatgaaaa gaccggaaag tcgcgactta ttcaagaaag     3480

cgaattgccc gaatggctgt tgaagcaaga cgatgaaatc tactcgtggg gccttgatga     3540

tccagatgct gttttaggaa ggggtagtag gcaaagaaaa gaagttgatt atgttgacag     3600

cctgacggag aaagagtggc ttaaggctat tgacgaagag ggagaatttg aggaagaaca     3660

agaaggtgat aaagaaggtc tcagaaagaa aagagggagg aagaggaaga agcgcgatga     3720

tgacgaagag gcaagccaaa ttaagagaag aaaggtgcat ctagccgaga tcaagatgaa     3780

gaaaaagatg aagaggctta tggaagttgt tgtgaactac agggacaggg atggtagagt     3840

attgagcgaa ccgtttatga aacttccatc aaagaaggag ttacctgagt attacgatac     3900

gattaagaaa cctattgata ttgaaaaagt cgttgccaac gtagaagaag gaaaatattt     3960

cacgatgcac gatttggaaa gagatttcga cttgctgtgc caaaacgccc aacaatacaa     4020

cgaagaagac tccatgatct acgaggacag cctcgttctt cgacaggtgt ttagaagcgc     4080

gagggaaaag atcgacggta cctcagacca cgacgacaac gccgatggac cggcggtggc     4140

tcagatcaaa cgacctcgtg gtagacctcg aaaacacaag agacccgaag agatcgaggc     4200

cgaagcggcg gctcagaaag ctatggagga ggcatcgaag ctgagagctc aagctgaggc     4260

ggaagagctt agatctaagg tggaggaggc atctcagaga gccaaagagg aagcgaaagc     4320

aagggaggaa gccaaagcta gggaagaagc cgaaatcgag aacatggagg agattcccac     4380

aagcacatga tctatagagc aaccggaaac aaaaaggcaa aaaagaaata ttatatagaa     4440

aagatgtaca tgttcaatgg agatacattt tcgccgagtt acaacgggta atgcttttac     4500

aacggatatt ttgacgtatg aatgttgacg ttcagatgaa gtatatttat aaaataatcc     4560

agacctttac gttttggttg atttgttttc tgtattgttc agtttattga acaaccatta     4620

atagcagctt acctaaatga tttagaaaag catctgagtt atttagataa gttttgagat     4680

tatatttatt aactttaata ttactatctt tattatagca tattgtaatt attttttcct     4740

gtccttcttt cgttgtgtgg tagataatcc gagagtcaac agttataagc aaatgaaatt     4800

cagttaaacc tcaaatgtac aaaatgatca aattaatgtt tacaatttat ttttttacca     4860

cgcacattca ctattactat tgtcagtcat tgagatatca ttttatatag ctccatgtct     4920

gtcttcctca atttacagag aagcaattag acaagtaatg acataatatg gtgctgaaat     4980

aatgtgcttg atagtgatgt tcacaaagta actattcgtt acaaagtact cgttacttac     5040

aaataccgaa actaacgatt actatacaga gaggcaaatc gttactttga ttacactgat     5100

tacttcgtat caatcgtatc agagcgagta acga                                 5134


<210>  104
<211>  451
<212>  DNA
<213>  Diabrotica virgifera


<220>
<221>  misc_feature
<222>  (119)..(119)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (128)..(128)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (289)..(289)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (403)..(403)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (414)..(414)
<223>  n is a, c, g, or t

<400>  104
attctggtta ataggaaagc ttaaggaata ttcaaattag agggttagaa tggatggtgt       60

ctttgtacaa taacaatctg aatggtattc tagcagatga gatgggtcta ggtaaaccnt      120

tcaaacgntt ggcttgttga cctatttgat ggaaaaaaag aagataaatg gaccgttttt      180

gatcatagtg ccactttcaa ccattctaat tggatagttg gaatttcaaa gtagggccct      240

actagtagtt gtcatttcat acaaaggctc tcctgtggtt agaaaagtna tccagagcca      300

gttaaaagct gctaaattca atgtgcttct cactacctac gagtacatta ttaaggcaag      360

gtgattagca aaaaatccca gtttaaatat atgatcatag atnaggtcat catnaaacac      420

actgcaattg aactcaaggc ctgaatacgc a                                     451


<210>  105
<211>  538
<212>  DNA
<213>  Diabrotica virgifera

<400>  105
agtgtattag caaaaatccc atttaaatat atgatcatag atgagggtca tcgtatgaaa       60

aaccaccact gcaaattgac tcaagtcctg aatacgcact atttggcgcc ctacagactc      120

ctgcttactg gtactcccct acaaaataaa ttaccagaat tatgggcctt gttgaatttc      180

ttgttgcctt cgattttcaa gagttgctcc acttttgaac aatggttcaa tgcgccattc      240

gcaacaacag gagaaaaggt tgagttaaac gaagaagaaa ctatccttat catccgtcgt      300

cttcacaaag tactcaggcc gtttctcctg agacgtctca agaaagaagt cgaatctcag      360

cttccagaca aagtggaata tatcataaag tgtgacatgt cgggcctaca aaaggttctc      420

tatgcacaca tgcagagcaa gggtgtgtta cttaccgatg gttccgagaa gggcagtaaa      480

ggaaggggat ctaaggacaa ctagatgaac accattatgc agctgaggaa actgtgct        538


<210>  106
<211>  303
<212>  DNA
<213>  Diabrotica virgifera

<400>  106
agggctggag gtctgggact taatttgcag acagctgata ctgtgattat cttcgattcc       60

gattggaatc ctcatcagga tttacaagct caggatcgag ctcatcgtat tggacagcaa      120

aatgaggtcc gagttttgcg tttgatgact gttaactctg ttgaggaacg aattttagct      180

gcagctaaat acaagcttac tatggacgaa aaggtcattc aagctggtat gttcgatcag      240

aagtctacgg gatctgaaag gcagcagttt cttcagagta ttttacacaa tgatggtagt      300

gat                                                                    303


<210>  107
<211>  14
<212>  DNA
<213>  Diabrotica virgifera

<400>  107
agggctggag gtct                                                         14


<210>  108
<211>  459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplified fragment

<400>  108
atgagggtca tcgtatgaaa aaccaccact gcaaattgac tcaagtcctg aatacgcact       60

atttggcgcc ctacagactc ctgcttactg gtactcccct acaaaataaa ttaccagaat      120

tatgggcctt gttgaatttc ttgttgcctt cgattttcaa gagttgctcc acttttgaac      180

aatggttcaa tgcgccattc gcaacaacag gagaaaaggt tgagttaaac gaagaagaaa      240

ctatccttat catccgtcgt cttcacaaag tactcaggcc gtttctcctg agacgtctca      300

agaaagaagt cgaatctcag cttccagaca aagtggaata tatcataaag tgtgacatgt      360

cgggcctaca aaaggttctc tatgcacaca tgcagagcaa gggtgtgtta cttaccgatg      420

gttccgagaa gggcagtaaa ggaaggggat ctaaggaca                             459


<210>  109
<211>  812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hairpin

<400>  109
gcgccctaca gactcctgct tactggtact cccctacaaa ataaattacc agaattatgg       60

gccttgttga atttcttgtt gccttcgatt ttcaagagtt gctccacttt tgaacaatgg      120

ttcaatgcgc cattcgcaac aacaggagaa aaggttgagt taaacgaaga agaaactatc      180

cttatcatcc gtcgtcttca caaagtactc aggccgtttc tcctgagacg tctcaagaaa      240

gaagtcgaat ctcagcttcc agacaaagtg gaatatatca taaagtgtga catgtgacta      300

gtaccggttg ggaaaggtat gtttctgctt ctacctttga tatatatata ataattatca      360

ctaattagta gtaatatagt atttcaagta tttttttcaa aataaaagaa tgtagtatat      420

agctattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat      480

atgaccaaaa catggtgatg tgcaggttga tccgcggaca tgtcacactt tatgatatat      540

tccactttgt ctggaagctg agattcgact tctttcttga gacgtctcag gagaaacggc      600

ctgagtactt tgtgaagacg acggatgata aggatagttt cttcttcgtt taactcaacc      660

ttttctcctg ttgttgcgaa tggcgcattg aaccattgtt caaaagtgga gcaactcttg      720

aaaatcgaag gcaacaagaa attcaacaag gcccataatt ctggtaattt attttgtagg      780

ggagtaccag taagcaggag tctgtagggc gc                                    812


<210>  110
<211>  167
<212>  PRT
<213>  Diabrotica virgifera

<400>  110

Ser Val Leu Ala Lys Ile Pro Phe Lys Tyr Met Ile Ile Asp Glu Gly 
1               5                   10                  15      


His Arg Met Lys Asn His His Cys Lys Leu Thr Gln Val Leu Asn Thr 
            20                  25                  30          


His Tyr Leu Ala Pro Tyr Arg Leu Leu Leu Thr Gly Thr Pro Leu Gln 
        35                  40                  45              


Asn Lys Leu Pro Glu Leu Trp Ala Leu Leu Asn Phe Leu Leu Pro Ser 
    50                  55                  60                  


Ile Phe Lys Ser Cys Ser Thr Phe Glu Gln Trp Phe Asn Ala Pro Phe 
65                  70                  75                  80  


Ala Thr Thr Gly Glu Lys Val Glu Leu Asn Glu Glu Glu Thr Ile Leu 
                85                  90                  95      


Ile Ile Arg Arg Leu His Lys Val Leu Arg Pro Phe Leu Leu Arg Arg 
            100                 105                 110         


Leu Lys Lys Glu Val Glu Ser Gln Leu Pro Asp Lys Val Glu Tyr Ile 
        115                 120                 125             


Ile Lys Cys Asp Met Ser Gly Leu Gln Lys Val Leu Tyr Ala His Met 
    130                 135                 140                 


Gln Ser Lys Gly Val Leu Leu Thr Asp Gly Ser Glu Lys Gly Ser Lys 
145                 150                 155                 160 


Gly Arg Gly Ser Lys Asp Asn 
                165         


