                         SEQUENCE LISTING

<110>  University of Exeter
 
<120>  Vaccine

<130>  RT/P2037PC00

<150>  GB1118394.4
<151>  2011-10-25

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  292
<212>  PRT
<213>  Clostridium perfringens

<400>  1

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  2
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Expression construct

<400>  2

Met Gly Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


Arg Trp Gly Ser Glu Leu Glu Ser Glu Leu Asn Asp Ile Asn Lys Ile 
        35                  40                  45              


Glu Leu Lys Asn Leu Ser Gly Glu Ile Ile Lys Glu Asn Gly Lys Glu 
    50                  55                  60                  


Ala Ile Lys Tyr Thr Ser Ser Asp Thr Ala Ser His Lys Gly Trp Lys 
65                  70                  75                  80  


Ala Thr Leu Ser Gly Thr Phe Ile Glu Asp Pro His Ser Asp Lys Lys 
                85                  90                  95      


Thr Ala Leu Leu Asn Leu Glu Gly Phe Ile Pro Ser Asp Lys Gln Ile 
            100                 105                 110         


Phe Gly Ser Lys Tyr Tyr Gly Lys Met Lys Trp Pro Glu Thr Tyr Arg 
        115                 120                 125             


Ile Asn Val Lys Ser Ala Asp Val Asn Asn Asn Ile Lys Ile Ala Asn 
    130                 135                 140                 


Ser Ile Pro Lys Asn Thr Ile Asp Lys Lys Asp Val Ser Asn Ser Ile 
145                 150                 155                 160 


Gly Tyr Ser Ile Gly Gly Asn Ile Ser Val Glu Gly Lys Thr Ala Gly 
                165                 170                 175     


Ala Gly Ile Asn Ala Ser Tyr Asn Val Gln Asn Thr Ile Ser Tyr Glu 
            180                 185                 190         


Gln Pro Asp Phe Arg Thr Ile Gln Arg Lys Asp Asp Ala Asn Leu Ala 
        195                 200                 205             


Ser Trp Asp Ile Lys Phe Val Glu Thr Lys Asp Gly Tyr Asn Ile Asp 
    210                 215                 220                 


Ser Tyr His Ala Ile Tyr Gly Asn Gln Leu Phe Met Lys Ser Arg Leu 
225                 230                 235                 240 


Tyr Asn Asn Gly Asp Lys Asn Phe Thr Asp Asp Arg Asp Leu Ser Thr 
                245                 250                 255     


Leu Ile Ser Gly Gly Phe Ser Pro Asn Met Ala Leu Ala Leu Thr Ala 
            260                 265                 270         


Pro Lys Asn Ala Lys Glu Ser Val Ile Ile Val Glu Tyr Gln Arg Phe 
        275                 280                 285             


Asp Asn Asp Tyr Ile Leu Asn Trp Glu Thr Thr Gln Trp Arg Gly Thr 
    290                 295                 300                 


Asn Lys Leu Ser Ser Thr Ser Glu Tyr Asn Glu Phe Met Phe Lys Ile 
305                 310                 315                 320 


Asn Trp Gln Asp His Lys Ile Glu Tyr Tyr Leu 
                325                 330     


<210>  3
<211>  322
<212>  PRT
<213>  Clostridium perfringens

<400>  3

Met Lys Arg Leu Lys Ile Ile Ser Ile Thr Leu Val Leu Thr Ser Val 
1               5                   10                  15      


Ile Ser Thr Ser Leu Phe Ser Thr Gln Thr Gln Val Phe Ala Ser Glu 
            20                  25                  30          


Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly Glu Ile 
        35                  40                  45              


Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser Asp Thr 
    50                  55                  60                  


Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe Ile Glu 
65                  70                  75                  80  


Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu Gly Phe 
                85                  90                  95      


Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly Lys Met 
            100                 105                 110         


Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp Val Asn 
        115                 120                 125             


Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile Asp Lys 
    130                 135                 140                 


Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn Ile Ser 
145                 150                 155                 160 


Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr Asn Val 
                165                 170                 175     


Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile Gln Arg 
            180                 185                 190         


Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val Glu Thr 
        195                 200                 205             


Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly Asn Gln 
    210                 215                 220                 


Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn Phe Thr 
225                 230                 235                 240 


Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser Pro Asn 
                245                 250                 255     


Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser Val Ile 
            260                 265                 270         


Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn Trp Glu 
        275                 280                 285             


Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser Glu Tyr 
    290                 295                 300                 


Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile Glu Tyr 
305                 310                 315                 320 


Tyr Leu 
        


<210>  4
<211>  969
<212>  DNA
<213>  Clostridium perfringens

<400>  4
atgaaaagat taaaaattat ttcaattaca ctagttctta caagtgtaat tagtacaagc       60

cttttttcaa ctcaaactca agtttttgca agtgaattaa atgacataaa caaaattgag      120

ttgaaaaatc taagtggaga aataataaaa gaaaatggaa aggaagctat taaatatact      180

tctagtgata ccgcttcaca taaaggttgg aaggcaactt taagtggaac atttattgaa      240

gatcctcatt ctgataagaa aactgcttta ttaaatttag aaggatttat accttctgat      300

aaacagattt ttggttctaa atattacgga aaaatgaaat ggcctgaaac ttatagaatt      360

aatgtaaaaa gtgctgatgt aaataataat ataaaaatag caaattctat tcctaaaaat      420

actatagata aaaaagatgt atctaattca attggttatt ctataggcgg taatatatct      480

gttgaaggaa aaactgctgg tgctggaata aatgcttcat ataatgtcca aaatactata      540

agctatgaac aacctgattt tagaacaatt caaagaaaag atgatgcaaa cttagcatca      600

tgggatataa aatttgttga gactaaggac ggttataata tagattctta tcatgctatt      660

tatggaaatc aattattcat gaaatcaaga ttgtataata atggtgataa aaatttcaca      720

gatgatagag atttatcaac attaatttct ggtggatttt cacccaatat ggctttagca      780

ttaacagcac ctaaaaatgc taaagaatct gtaataatag ttgaatatca aagatttgat      840

aatgactata ttttaaattg ggaaactact caatggcgag gaacaaacaa actttcgtca      900

acaagtgaat ataacgaatt tatgtttaaa ataaattggc aagatcataa aatagaatat      960

tatctgtaa                                                              969


<210>  5
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated sequence

<400>  5

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Ala Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  6
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated sequence

<400>  6

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Ala Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  7
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  7
cccgggctcg agagtgaatt aaatgacata aac                                    33


<210>  8
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  8
cccgggaagc ttttacagat aatattctat tttatg                                 36


<210>  9
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  9
ggaacattta ttgaagctcc tcattctgat aagaaaactg c                           41


<210>  10
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  10
gcagttttct tatcagaatg aggagcttca ataaatgttc c                           41


<210>  11
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  11
gcaaattcta ttgctaaaaa tactatagat aaaaaagatg tatc                        44


<210>  12
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  12
gatacatctt ttttatctat agtattttta gcaatagaat ttgc                        44


<210>  13
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  13
gatgtatcta attcaattgg tgcgtctata ggcgg                                  35


<210>  14
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  14
ccgcctatag acgcaccaat tgaattagat acatc                                  35


<210>  15
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  15
caattggtta ttctataggc gctaatatat ctgttgaagg                             40


<210>  16
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  16
ccttcaacag atatattagc gcctatagaa taaccaattg                             40


<210>  17
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  17
gtccaaaata ctataagcgc tgaacaacct gattttagaa c                           41


<210>  18
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  18
gttctaaaat caggttgttc agcgcttata gtattttgga c                           41


<210>  19
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  19
ccaaaatact ataagctatg aagcacctga ttttagaaca attc                        44


<210>  20
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  20
gaattgttct aaaatcaggt gcttcatagc ttatagtatt ttgg                        44


<210>  21
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  21
ctataagcta tgaacaagct gattttagaa caattcaaag                             40


<210>  22
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  22
ctttgaattg ttctaaaatc agcttgttca tagcttatag                             40


<210>  23
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  23
caattattca tgaaatcagc attgtataat aatggtg                                37


<210>  24
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  24
caccattatt atacaatgct gatttcatga ataattg                                37


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than Y

<400>  25

Asn Thr Ile Ser Xaa Glu Gln Pro Asp Phe 
1               5                   10  


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Any amino acid other than Y

<400>  26

Ser Tyr Asn Val Gln Asn Thr Ile Ser Xaa Glu Gln Pro Asp Phe Arg 
1               5                   10                  15      


Thr Ile Gln Arg 
            20  


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than P

<400>  27

Ala Asn Ser Ile Xaa Lys Asn Thr Ile Asp 
1               5                   10  


<210>  28
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  Xaa
<222>  (10)..(10)
<223>  any amino acid other than P

<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Any amino acid other than P

<400>  28

Asn Asn Ile Lys Ile Ala Asn Ser Ile Xaa Lys Asn Thr Ile Asp Lys 
1               5                   10                  15      


Lys Asp Val Ser 
            20  


<210>  29
<211>  68
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide fragment

<400>  29

Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile Asp 
1               5                   10                  15      


Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn Ile 
            20                  25                  30          


Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr Asn 
        35                  40                  45              


Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile Gln 
    50                  55                  60                  


Arg Lys Asp Asp 
65              


<210>  30
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide fragment

<400>  30

Pro Lys Asn Thr Ile Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr 
1               5                   10                  15      


Ser Ile Gly Gly Asn Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly 
            20                  25                  30          


Ile Asn Ala Ser Tyr Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro 
        35                  40                  45              


<210>  31
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant encoding DNA

<400>  31
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tcctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agcgctgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt tatggaaatc aattattcat gaaatcaaga      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaattg ggaaactact      780

caatggcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


<210>  32
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant encoding DNA

<400>  32
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tgctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agctatgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt tatggaaatc aattattcat gaaatcaaga      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaattg ggaaactact      780

caatggcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


<210>  33
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encoding mature NetB

<400>  33
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tcctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agctatgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt tatggaaatc aattattcat gaaatcaaga      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaattg ggaaactact      780

caatggcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


<210>  34
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide fragment

<400>  34

Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly Asn Gln Leu Phe Met 
1               5                   10                  15      


Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn Phe Thr Asp Asp Arg 
            20                  25                  30          


Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser Pro Asn Met Ala Leu 
        35                  40                  45              


Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser Val Ile Ile Val Glu 
    50                  55                  60                  


Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn Trp Glu Thr Thr Gln 
65                  70                  75                  80  


Trp Arg Gly Thr Asn Lys 
                85      


<210>  35
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide fragment

<400>  35

Tyr His Ala Ile Tyr Gly Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr 
1               5                   10                  15      


Asn Asn Gly Asp Lys Asn Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu 
            20                  25                  30          


Ile Ser Gly Gly Phe Ser Pro Asn Met Ala Leu Ala Leu Thr Ala Pro 
        35                  40                  45              


Lys Asn Ala Lys Glu Ser Val Ile Ile Val Glu Tyr Gln Arg Phe Asp 
    50                  55                  60                  


Asn Asp Tyr Ile Leu Asn Trp Glu Thr Thr Gln Trp 
65                  70                  75      


<210>  36
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated polypeptide sequence

<400>  36

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Ala Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  37
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated polypeptide sequence

<400>  37

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Ala Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  38
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated polypeptide sequence

<400>  38

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Ala Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  39
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated polypeptide sequence

<400>  39

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Ala Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  40
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (5)..(5)
<223>  Any amino acid other than Y

<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than Y

<400>  40

Tyr His Ala Ile Xaa Gly Asn Gln Leu Phe 
1               5                   10  


<210>  41
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (10)..(10)
<223>  Any amino acid other than Y

<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Any amino acid other than Y

<400>  41

Tyr Asn Ile Asp Ser Tyr His Ala Ile Xaa Gly Asn Gln Leu Phe Met 
1               5                   10                  15      


Lys Ser Arg Leu 
            20  


<210>  42
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (5)..(5)
<223>  Any amino acid other than R

<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than R

<400>  42

Phe Met Lys Ser Xaa Leu Tyr Asn Asn Gly 
1               5                   10  


<210>  43
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (10)..(10)
<223>  Any amino acid other than R

<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Any amino acid other than R

<400>  43

Tyr Gly Asn Gln Leu Phe Met Lys Ser Xaa Leu Tyr Asn Asn Gly Asp 
1               5                   10                  15      


Lys Asn Phe Thr 
            20  


<210>  44
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (5)..(5)
<223>  Any amino acid other than W

<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than W

<400>  44

Tyr Ile Leu Asn Xaa Glu Thr Thr Gln Trp 
1               5                   10  


<210>  45
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (10)..(10)
<223>  Any amino acid other than W.

<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Any amino acid other than W.

<400>  45

Arg Phe Asp Asn Asp Tyr Ile Leu Asn Xaa Glu Thr Thr Gln Trp Arg 
1               5                   10                  15      


Gly Thr Asn Lys 
            20  


<210>  46
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (5)..(5)
<223>  Any amino acid other than W

<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than W

<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Any amino acid other than W

<400>  46

Glu Thr Thr Gln Xaa Arg Gly Thr Asn Lys 
1               5                   10  


<210>  47
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant peptide fragment


<220>
<221>  X
<222>  (10)..(10)
<223>  Any amino acid other than W

<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Any amino acid other than W

<400>  47

Tyr Ile Leu Asn Trp Glu Thr Thr Gln Xaa Arg Gly Thr Asn Lys Leu 
1               5                   10                  15      


Ser Ser Thr Ser 
            20  


<210>  48
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant encoding DNA

<400>  48
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tcctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agctatgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt gctggaaatc aattattcat gaaatcaaga      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaattg ggaaactact      780

caatggcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


<210>  49
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant encoding DNA

<400>  49
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tcctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agctatgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt tatggaaatc aattattcat gaaatcagca      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaattg ggaaactact      780

caatggcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


<210>  50
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant encoding DNA

<400>  50
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tcctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agctatgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt tatggaaatc aattattcat gaaatcaaga      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaatgc ggaaactact      780

caatggcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


<210>  51
<211>  879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant encoding DNA

<400>  51
agtgaattaa atgacataaa caaaattgag ttgaaaaatc taagtggaga aataataaaa       60

gaaaatggaa aggaagctat taaatatact tctagtgata ccgcttcaca taaaggttgg      120

aaggcaactt taagtggaac atttattgaa gatcctcatt ctgataagaa aactgcttta      180

ttaaatttag aaggatttat accttctgat aaacagattt ttggttctaa atattacgga      240

aaaatgaaat ggcctgaaac ttatagaatt aatgtaaaaa gtgctgatgt aaataataat      300

ataaaaatag caaattctat tcctaaaaat actatagata aaaaagatgt atctaattca      360

attggttatt ctataggcgg taatatatct gttgaaggaa aaactgctgg tgctggaata      420

aatgcttcat ataatgtcca aaatactata agctatgaac aacctgattt tagaacaatt      480

caaagaaaag atgatgcaaa tttagcatca tgggatataa aatttgttga gactaaggac      540

ggttataata tagattctta tcatgctatt tatggaaatc aattattcat gaaatcaaga      600

ttgtataata atggtgataa aaatttcaca gatgatagag atttatcaac attaatttct      660

ggtggatttt cacccaatat ggctttagca ttaacagcac ctaaaaatgc taaagaatct      720

gtaataatag ttgaatatca aagatttgat aatgactata ttttaaattg ggaaactact      780

caagcgcgag gaacaaacaa actttcgtca acaagtgaat ataacgaatt tatgtttaaa      840

ataaattggc aagatcataa aatagaatat tatctgtaa                             879


