               SEQUENCE LISTING

<110> CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

<120> EFECTO ADYUVANTE DE LA PROTEÍNA A27 DEL VIRUS VACCINIA (14K) Y SUS APLICACIONES EN VACUNAS

<130> 5110098 MARIANO ESTEBAN

<160> 15

<170> BiSSAP 1.0

<210> 1
<211> 450
<212> DNA
<213> Vaccinia virus WR

<220> 
<221> source
<222> 1..450
<223> /mol_type="DNA"
      /note="VACFP14KD Vaccinia virus 14kd fusion protein gene, complet
      e cds"
      /organism="Vaccinia virus WR"

<400> 1
aatataaata acgcagagtg tcagtttcta aaatctgtac tttaaatgga cggaactctt      60

ttccccggag atgacgatct tgcaattcca gcaactgaat ttttttctac aaaggctgct     120

aaaaagccag aggctaaacg cgaagcaatt gttaaagccg atgaagacga caatgaggaa     180

actctcaaac aacggctaac taatttggaa aaaaagatta ctaatgtaac aacaaagttt     240

gaacaaatag aaaagtgttg taaacgcaac gatgaagttc tatttaggtt ggaaaatcac     300

gctgaaactc taagagcggc tatgatatct ctggctaaaa agattgatgt tcagactgga     360

cggcgcccat atgagtaact taactctttt gttaattaaa agtatattca aaaaatgagt     420

tatataaatg gcgaacatta taaatttatg                                      450


<210> 2
<211> 110
<212> PRT
<213> Vaccinia virus WR

<220> 
<221> SOURCE
<222> 1..110
<223> /mol_type="protein"
      /note="14k membrane protein"
      /organism="Vaccinia virus WR"

<400> 2
Met Asp Gly Thr Leu Phe Pro Gly Asp Asp Asp Leu Ala Ile Pro Ala 
1               5                   10                   15    
Thr Glu Phe Phe Ser Thr Thr Ala Ala Lys Lys Pro Glu Ala Lys Arg 
            20                   25                  30        
Glu Ala Ile Val Lys Ala Asp Glu Asp Asp Asn Glu Glu Thr Leu Lys 
        35                   40                  45            
Gln Arg Leu Thr Asn Leu Glu Lys Lys Ile Thr Asn Val Thr Thr Lys 
    50                   55                  60                
Phe Glu Gln Ile Glu Lys Cys Cys Lys Arg Asn Asp Glu Val Leu Phe 
65                   70                  75                  80
Arg Leu Glu Asn His Ala Glu Thr Leu Arg Ala Ala Met Ile Ser Leu 
                85                   90                  95    
Ala Lys Lys Ile Asp Val Gln Thr Gly Arg Arg Pro Tyr Glu 
            100                  105                110

<210> 3
<211> 367
<212> PRT
<213> Plasmodium yoelii

<220> 
<221> SOURCE
<222> 1..367
<223> /mol_type="protein"
      /note="circumsporozoite protein"
      /organism="Plasmodium yoelii"

<400> 3
Met Lys Lys Cys Thr Ile Leu Val Val Ala Ser Leu Leu Leu Val Asp 
1               5                   10                   15    
Ser Leu Leu Pro Gly Tyr Gly Gln Asn Lys Ser Val Gln Ala Gln Arg 
            20                   25                  30        
Asn Leu Asn Glu Leu Cys Tyr Asn Glu Glu Asn Asp Asn Lys Leu Tyr 
        35                   40                  45            
His Val Leu Asn Ser Lys Asn Gly Lys Ile Tyr Asn Arg Asn Ile Val 
    50                   55                  60                
Asn Arg Leu Leu Gly Asp Ala Leu Asn Gly Lys Pro Glu Glu Lys Lys 
65                   70                  75                  80
Asp Asp Pro Pro Lys Asp Gly Asn Lys Asp Asp Leu Pro Lys Glu Glu 
                85                   90                  95    
Lys Lys Asp Asp Leu Pro Lys Glu Glu Lys Lys Asp Asp Pro Pro Lys 
            100                  105                110        
Asp Pro Lys Lys Asp Asp Pro Pro Lys Glu Ala Gln Asn Lys Leu Asn 
        115                  120                125            
Gln Pro Val Val Ala Asp Glu Asn Val Asp Gln Gly Pro Gly Ala Pro 
    130                  135                140                
Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly 
145                  150                155                  160
Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly 
                165                  170                175    
Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro 
            180                  185                190        
Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly 
        195                  200                205            
Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly 
    210                  215                220                
Pro Gly Ala Pro Gln Glu Pro Pro Gln Gln Pro Pro Gln Gln Pro Pro 
225                  230                235                  240
Gln Gln Pro Pro Gln Gln Pro Pro Gln Gln Pro Pro Gln Gln Pro Pro 
                245                  250                255    
Gln Gln Pro Arg Pro Gln Pro Asp Gly Asn Asn Asn Asn Asn Asn Asn 
            260                  265                270        
Asn Gly Asn Asn Asn Glu Asp Ser Tyr Val Pro Ser Ala Glu Gln Ile 
        275                  280                285            
Leu Glu Phe Val Lys Gln Ile Ser Ser Gln Leu Thr Glu Glu Trp Ser 
    290                  295                300                
Gln Cys Ser Val Thr Cys Gly Ser Gly Val Arg Val Arg Lys Arg Lys 
305                  310                315                  320
Asn Val Asn Lys Gln Pro Glu Asn Leu Thr Leu Glu Asp Ile Asp Thr 
                325                  330                335    
Glu Ile Cys Lys Met Asp Lys Cys Ser Ser Ile Phe Asn Ile Val Ser 
            340                  345                350        
Asn Ser Leu Gly Phe Val Ile Leu Leu Val Leu Val Phe Phe Asn 
        355                  360                365        

<210> 4
<211> 442
<212> PRT
<213> Plasmodium falciparum

<220> 
<221> SOURCE
<222> 1..442
<223> /mol_type="protein"
      /note="circumsporozoite protein"
      /organism="Plasmodium falciparum"

<400> 4
Met Met Arg Lys Leu Ala Ile Leu Ser Val Ser Ser Phe Leu Phe Val 
1               5                   10                   15    
Glu Ala Leu Phe Gln Glu Tyr Gln Cys Tyr Gly Ser Ser Ser Asn Thr 
            20                   25                  30        
Arg Val Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr Asn Leu Tyr 
        35                   40                  45            
Asn Glu Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn Trp Tyr Ser 
    50                   55                  60                
Leu Lys Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp Gly Asp Asn 
65                   70                  75                  80
Asp Asn Gly Asn Asn Asn Asn Gly Asn Asn Asn Asn Gly Asp Asn Gly 
                85                   90                  95    
Arg Glu Gly Lys Asp Glu Asp Lys Arg Asp Gly Asn Asn Glu Asp Asn 
            100                  105                110        
Glu Lys Leu Arg Lys Pro Lys His Lys Lys Leu Lys Gln Pro Gly Asp 
        115                  120                125            
Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn 
    130                  135                140                
Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
145                  150                155                  160
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
                165                  170                175    
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
            180                  185                190        
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
        195                  200                205            
Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
    210                  215                220                
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
225                  230                235                  240
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
                245                  250                255    
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
            260                  265                270        
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
        275                  280                285            
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
    290                  295                300                
Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Lys Asn 
305                  310                315                  320
Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro Asn Arg 
                325                  330                335    
Asn Val Asp Glu Asn Ala Asn Ala Asn Asn Ala Val Lys Asn Asn Asn 
            340                  345                350        
Asn Glu Glu Pro Ser Asp Lys His Ile Glu Gln Tyr Leu Lys Lys Ile 
        355                  360                365            
Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys Gly 
    370                  375                380                
Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asp Lys Pro Lys 
385                  390                395                  400
Asp Gln Leu Asp Tyr Glu Asn Asp Ile Glu Lys Lys Ile Cys Lys Met 
                405                  410                415    
Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser Ser Ile Gly Leu 
            420                  425                430        
Ile Met Val Leu Ser Phe Leu Phe Leu Asn 
        435                  440        

<210> 5
<211> 398
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..398
<223> /mol_type="protein"
      /note="CS14K"
      /organism="Artificial Sequence"

<400> 5
Met Cys Tyr Asn Glu Glu Asn Asp Asn Lys Leu Tyr His Val Leu Asn 
1               5                   10                   15    
Ser Lys Asn Gly Lys Ile Tyr Asn Arg Asn Ile Val Asn Arg Leu Leu 
            20                   25                  30        
Gly Asp Ala Leu Asn Gly Lys Pro Glu Glu Lys Lys Asp Asp Pro Pro 
        35                   40                  45            
Lys Asp Gly Asn Lys Asp Asp Leu Pro Lys Glu Glu Lys Lys Asp Asp 
    50                   55                  60                
Leu Pro Lys Glu Glu Lys Lys Asp Asp Pro Pro Lys Asp Pro Lys Lys 
65                   70                  75                  80
Asp Asp Pro Pro Lys Glu Ala Gln Asn Lys Leu Asn Gln Pro Val Val 
                85                   90                  95    
Ala Asp Glu Asn Val Asp Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly 
            100                  105                110        
Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly 
        115                  120                125            
Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro 
    130                  135                140                
Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly 
145                  150                155                  160
Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly 
                165                  170                175    
Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro Gln Gly Pro Gly Ala Pro 
            180                  185                190        
Gln Glu Pro Pro Gln Gln Pro Pro Gln Gln Pro Pro Gln Gln Pro Pro 
        195                  200                205            
Gln Gln Pro Pro Gln Gln Pro Pro Gln Gln Pro Pro Gln Gln Pro Arg 
    210                  215                220                
Pro Gln Pro Asp Gly Asn Asn Asn Asn Asn Asn Asn Asn Gly Asn Asn 
225                  230                235                  240
Asn Glu Asp Ser Tyr Val Pro Ser Ala Glu Gln Ile Leu Glu Phe Val 
                245                  250                255    
Lys Gln Ile Ser Ser Gln Leu Thr Glu Glu Trp Ser Gln Cys Ser Val 
            260                  265                270        
Thr Cys Gly Ser Gly Val Arg Val Arg Lys Arg Lys Asn Val Asn Lys 
        275                  280                285            
Gln Pro Glu Asn Leu Thr Leu Glu Asp Ile Asp Thr Glu Ile Cys Lys 
    290                  295                300                
Met Asp Lys Cys Ser Ser Ile Phe Asn Ser Gly Arg Glu Ala Lys Arg 
305                  310                315                  320
Glu Ala Ile Val Lys Ala Asp Glu Asp Asp Asn Glu Glu Thr Leu Lys 
                325                  330                335    
Gln Arg Leu Thr Asn Leu Glu Lys Lys Ile Thr Asn Val Thr Thr Lys 
            340                  345                350        
Phe Glu Gln Ile Glu Lys Cys Cys Lys Arg Asn Asp Glu Val Leu Phe 
        355                  360                365            
Arg Leu Glu Asn His Ala Glu Thr Leu Arg Ala Ala Met Ile Ser Leu 
    370                  375                380                
Ala Lys Lys Ile Asp Val Gln Thr Gly Arg Arg Pro Tyr Glu 
385                  390                395            

<210> 6
<211> 449
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..449
<223> /mol_type="protein"
      /note="PfCS14K"
      /organism="Artificial Sequence"

<400> 6
Met Phe Gln Glu Tyr Gln Cys Tyr Gly Ser Ser Ser Asn Thr Arg Val 
1               5                   10                   15    
Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr Asn Leu Tyr Asn Glu 
            20                   25                  30        
Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn Trp Tyr Ser Leu Lys 
        35                   40                  45            
Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp Gly Asn Asn Glu Asp 
    50                   55                  60                
Asn Glu Lys Leu Arg Lys Pro Lys His Lys Lys Leu Lys Gln Pro Ala 
65                   70                  75                  80
Asp Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala 
                85                   90                  95    
Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala 
            100                  105                110        
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        115                  120                125            
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    130                  135                140                
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
145                  150                155                  160
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
                165                  170                175    
Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
            180                  185                190        
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        195                  200                205            
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    210                  215                220                
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
225                  230                235                  240
Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Lys 
                245                  250                255    
Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro Asn 
            260                  265                270        
Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys Asn Asn 
        275                  280                285            
Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys 
    290                  295                300                
Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys 
305                  310                315                  320
Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro 
                325                  330                335    
Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys 
            340                  345                350        
Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser Gly Arg Glu 
        355                  360                365            
Ala Lys Arg Glu Ala Ile Val Lys Ala Asp Glu Asp Asp Asn Glu Glu 
    370                  375                380                
Thr Leu Lys Gln Arg Leu Thr Asn Leu Glu Lys Lys Ile Thr Asn Val 
385                  390                395                  400
Thr Thr Lys Phe Glu Gln Ile Glu Lys Cys Cys Lys Arg Asn Asp Glu 
                405                  410                415    
Val Leu Phe Arg Leu Glu Asn His Ala Glu Thr Leu Arg Ala Ala Met 
            420                  425                430        
Ile Ser Leu Ala Lys Lys Ile Asp Val Gln Thr Gly Arg Arg Pro Tyr 
        435                  440                445            
Glu 
    

<210> 7
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..9
<223> /mol_type="protein"
      /note="Peptido de CS"
      /organism="Artificial Sequence"

<400> 7
Ser Tyr Val Pro Ser Ala Glu Gln Ile 
1               5                

<210> 8
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..33
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen CS"
      /organism="Artificial Sequence"

<400> 8
acttagaatt catgtgttac aatgaagaaa atg                                  33


<210> 9
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..29
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen CS"
      /organism="Artificial Sequence"

<400> 9
attgcggccg ctattaaata tacttgaac                                      29


<210> 10
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..31
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen A27L
      "
      /organism="Artificial Sequence"

<400> 10
gctgctagcg gccgcgaggc taaacgcgaa g                                   31


<210> 11
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..27
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen A27L
      "
      /organism="Artificial Sequence"

<400> 11
ccctcgagtg ggttactcat atggacg                                        27


<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen iNOS
      "
      /organism="Artificial Sequence"

<400> 12
tgggaatgga gactgtccca g                                              21


<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen iNOS
      "
      /organism="Artificial Sequence"

<400> 13
gggatctgaa tgtgatgttt g                                              21


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen IL-1
      2p40"
      /organism="Artificial Sequence"

<400> 14
aactggcgtt ggaagcacgg                                                20


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /note="Oligonucleotido para la amplificacion por PCR del gen IL-1
      2p40"
      /organism="Artificial Sequence"

<400> 15
gaacacatgc ccacttgctg                                                20


