                         SEQUENCE LISTING

<110>  The Trustees of Columbia University in the City of New York
       Xu, Dingbang
 
<120>  Selective Amplification and Detection of Mutant Gene Alleles

<130>  0344404

<150>  61/561703
<151>  2011-11-18

<150>  61/673933
<151>  2012-07-20

<160>  70    

<170>  PatentIn version 3.5

<210>  1
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  1
cgttgaagaa gtacaaaatg tcattaatgc tatgcagaaa agctta                      46


<210>  2
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  2
cttgtgctga cttaccagat ggggcact                                          28


<210>  3
<211>  11
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  3
gcttagagtg c                                                            11


<210>  4
<211>  9
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  4
gcttagtgc                                                                9


<210>  5
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  5
aatggaagaa accaccaagg tccaaagcga gcaagagcat                             40


<210>  6
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  6
acttgaggga gggagcttta cctttctggc ctg                                    33


<210>  7
<211>  11
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  7
gcatcccagg c                                                            11


<210>  8
<211>  12
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  8
gcatccccag gc                                                           12


<210>  9
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  9
ctgcaaatac ttgtgggatt tttagcacag ccag                                   34


<210>  10
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  10
gacttgcttg gtactatctt ctatttcaga aaaca                                  35


<210>  11
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  11
cagtg                                                                    5


<210>  12
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  12
caggg                                                                    5


<210>  13
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  13
gctgaaaatg actgaatata aacttgtggt agttggaact g                           41


<210>  14
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  14
atattcgtcc acaaaatgat tctgaattag ctgta                                  35


<210>  15
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  15
actggg                                                                   6


<210>  16
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  16
actgag                                                                   6


<210>  17
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  17
ttcttcatga agacctcaca gtaaaaatag gtgat                                  35


<210>  18
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  18
acaaaatgga tccagacaac tgttcaaact gat                                    33


<210>  19
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  19
cagtg                                                                    5


<210>  20
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  20
cagag                                                                    5


<210>  21
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  21
gagacaatga attaagggaa aatgacaa                                          28


<210>  22
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  22
cactg                                                                    5


<210>  23
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  23
ctgtgactcc atagaaaatc tttctc                                            26


<210>  24
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  24
cacta                                                                    5


<210>  25
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  25
cgtactggtg aaaacaccgc agcatgtcaa gatcacagat tttggcc                     47


<210>  26
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  26
cctgg                                                                    5


<210>  27
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  27
gtgtaaaatt gattccaatg ccatccactt gata                                   34


<210>  28
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  28
ccggg                                                                    5


<210>  29
<211>  49
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  29
tctgtcctgg gattctcttg agatgtggtc aatggaagaa accaccaag                   49


<210>  30
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  30
cctgggattc tcttgagatg tggtcaat                                          28


<210>  31
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  31
cccatctctg caaaggggag tggaa                                             25


<210>  32
<211>  81
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  32
tctgtcctgg gattctcttg agatgtggtc aatggaagaa accaccaagg tccaaagcga       60

gcaagagaat cccaggacag a                                                 81


<210>  33
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  33
tctgtcctgg gattctcttg agatgtggtc aatggaagaa accaccaagg tccaaagcga       60

gcaagagaat ccccaggaca ga                                                82


<210>  34
<211>  49
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  34
gggacactct aagattttct tcgcgttgaa gaagtacaaa atgtcatta                   49


<210>  35
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  35
acactctaag attttcttcg cgtt                                              24


<210>  36
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  36
aggtcaattc tgttcatttg cataggagat a                                      31


<210>  37
<211>  78
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  37
gggacactct aagattttct tcgcgttgaa gaagtacaaa atgtcattaa tgctatgcag       60

aaaatcttag agtgtccc                                                     78


<210>  38
<211>  76
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  38
gggacactct aagattttct tcgcgttgaa gaagtacaaa atgtcattaa tgctatgcag       60

aaaatcttag tgtccc                                                       76


<210>  39
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  39
gacagatttt ccacttgctg tgcggaagct tcataagtca gtctcatctg caaatac          57


<210>  40
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  40
attttccact tgctgtgcgg aagcttcata                                        30


<210>  41
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  41
gtactatctt ctatttcaga aaacacttgt cttgcgt                                37


<210>  42
<211>  93
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  42
gacagatttt ccacttgctg tgcggaagct tcataagtca gtctcatctg caaatactgt       60

gggattttta gcacagcaag tggaaaatct gtc                                    93


<210>  43
<211>  92
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  43
gacagatttt ccacttgctg tgcggaagct tcataagtca gtctcatctg caaatactgt       60

gggattttta gcacagcaag ggaaaatctg tc                                     92


<210>  44
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  44
tctctttgat gtcacgttca ccaccacagc tgagagggaa at                          42


<210>  45
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  45
tctctttgat gtcacgttca cc                                                22


<210>  46
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  46
ggaattgaat gtagtttcat ggat                                              24


<210>  47
<211>  1889
<212>  DNA
<213>  Mus musculus

<400>  47
ctgtcgagtc gcgtccaccc gcgagcacag cttctttgca gctccttcgt tgccggtcca       60

cacccgccac cagttcgcca tggatgacga tatcgctgcg ctggtcgtcg acaacggctc      120

cggcatgtgc aaagccggct tcgcgggcga cgatgctccc cgggctgtat tcccctccat      180

cgtgggccgc cctaggcacc agggtgtgat ggtgggaatg ggtcagaagg actcctatgt      240

gggtgacgag gcccagagca agagaggtat cctgaccctg aagtacccca ttgaacatgg      300

cattgttacc aactgggacg acatggagaa gatctggcac cacaccttct acaatgagct      360

gcgtgtggcc cctgaggagc accctgtgct gctcaccgag gcccccctga accctaaggc      420

caaccgtgaa aagatgaccc agatcatgtt tgagaccttc aacaccccag ccatgtacgt      480

agccatccag gctgtgctgt ccctgtatgc ctctggtcgt accacaggca ttgtgatgga      540

ctccggagac ggggtcaccc acactgtgcc catctacgag ggctatgctc tccctcacgc      600

catcctgcgt ctggacctgg ctggccggga cctgacagac tacctcatga agatcctgac      660

cgagcgtggc tacagcttca ccaccacagc tgagagggaa atcgtgcgtg acatcaaaga      720

gaagctgtgc tatgttgctc tagacttcga gcaggagatg gccactgccg catcctcttc      780

ctccctggag aagagctatg agctgcctga cggccaggtc atcactattg gcaacgagcg      840

gttccgatgc cctgaggctc ttttccagcc ttccttcttg ggtatggaat cctgtggcat      900

ccatgaaact acattcaatt ccatcatgaa gtgtgacgtt gacatccgta aagacctcta      960

tgccaacaca gtgctgtctg gtggtaccac catgtaccca ggcattgctg acaggatgca     1020

gaaggagatt actgctctgg ctcctagcac catgaagatc aagatcattg ctcctcctga     1080

gcgcaagtac tctgtgtgga tcggtggctc catcctggcc tcactgtcca ccttccagca     1140

gatgtggatc agcaagcagg agtacgatga gtccggcccc tccatcgtgc accgcaagtg     1200

cttctaggcg gactgttact gagctgcgtt ttacaccctt tctttgacaa aacctaactt     1260

gcgcagaaaa aaaaaaaata agagacaaca ttggcatggc tttgtttttt taaatttttt     1320

ttaaagtttt tttttttttt tttttttttt tttttaagtt tttttgtttt gttttggcgc     1380

ttttgactca ggatttaaaa actggaacgg tgaaggcgac agcagttggt tggagcaaac     1440

atcccccaaa gttctacaaa tgtggctgag gactttgtac attgttttgt tttttttttt     1500

ttttggtttt gtcttttttt aatagtcatt ccaagtatcc atgaaataag tggttacagg     1560

aagtccctca ccctcccaaa agccaccccc actcctaaga ggaggatggt cgcgtccatg     1620

ccctgagtcc accccgggga aggtgacagc attgcttctg tgtaaattat gtactgcaaa     1680

aattttttta aatcttccgc cttaatactt catttttgtt tttaatttct gaatggccca     1740

ggtctgaggc ctcccttttt tttgtccccc caacttgatg tatgaaggct ttggtctccc     1800

tgggaggggg ttgaggtgtt gaggcagcca gggctggcct gtacactgac ttgagaccaa     1860

taaaagtgca caccttacct tacacaaac                                       1889


<210>  48
<211>  1296
<212>  DNA
<213>  Rattus norvegicus

<400>  48
ggggtcgagt ccgcgtccac ccgcgagtac aaccttcttg cagctcctcc gtcgccggtc       60

cacacccgcc accagttcgc catggatgac gatatcgctg cgctcgtcgt cgacaacggc      120

tccggcatgt gcaaggccgg cttcgcgggc gacgatgctc cccgggccgt cttcccctcc      180

atcgtgggcc gccctaggca ccagggtgtg atggtgggta tgggtcagaa ggactcctac      240

gtgggcgacg aggcccagag caagagaggc atcctgaccc tgaagtaccc cattgaacac      300

ggcattgtca ccaactggga cgatatggag aagatttggc accacacttt ctacaatgag      360

ctgcgtgtgg cccctgagga gcaccctgtg ctgctcaccg aggcccctct gaaccctaag      420

gccaaccgtg aaaagatgac ccagatcatg tttgagacct tcaacacccc agccatgtac      480

gtagccatcc aggctgtgtt gtccctgtat gcctctggtc gtaccactgg cattgtgatg      540

gactccggag acggggtcac ccacactgtg cccatctatg agggttacgc gctccctcat      600

gccatcctgc gtctggacct ggctggccgg gacctgacag actacctcat gaagatcctg      660

accgagcgtg gctacagctt caccaccaca gctgagaggg aaatcgtgcg tgacattaaa      720

gagaagctgt gctatgttgc cctagacttc gagcaagaga tggccactgc cgcatcctct      780

tcctccctgg agaagagcta tgagctgcct gacggtcagg tcatcactat cggcaatgag      840

cggttccgat gccccgaggc tctcttccag ccttccttcc tgggtatgga atcctgtggc      900

atccatgaaa ctacattcaa ttccatcatg aagtgtgacg ttgacatccg taaagacctc      960

tatgccaaca cagtgctgtc tggtggcacc accatgtacc caggcattgc tgacaggatg     1020

cagaaggaga ttactgccct ggctcctagc accatgaaga tcaagatcat tgctcctcct     1080

gagcgcaagt actctgtgtg gattggtggc tctatcctgg cctcactgtc caccttccag     1140

cagatgtgga tcagcaagca ggagtacgat gagtccggcc cctccatcgt gcaccgcaaa     1200

tgcttctagg cggactgtta ctgagctgcg ttttacaccc tttctttgac aaaacctaac     1260

ttgcgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa                               1296


<210>  49
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  49
catttacatc tttcaattat gtatattctt                                        30


<210>  50
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  50
atgcaaatac ttcattagta aaatttactt                                        30


<210>  51
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  51
cttattgact ctaagaggaa agatgaagta ctatg                                  35


<210>  52
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  52
caattcttac catccacaaa atggatccag                                        30


<210>  53
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  53
cttattgact ctaagaggaa agatgaagta ctatg                                  35


<210>  54
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  54
caattcttac catccacaaa atggatccag                                        30


<210>  55
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  55
ctgcaaatac ttgtgggatt tttagcacag ccag                                   34


<210>  56
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  56
gacttgcttg gtactatctt ctatttcaga aaaca                                  35


<210>  57
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  57
gagacaatga attaagggaa aatgacaa                                          28


<210>  58
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  58
ctgtgactcc atagaaaatc tttctc                                            26


<210>  59
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  59
gctgaaaatg actgaatata aacttgtggt agttggaact g                           41


<210>  60
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  60
atattcgtcc acaaaatgat tctgaattag ctgta                                  35


<210>  61
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  61
gactgaatat aaacttgtgg tagttggcgc tggtg                                  35


<210>  62
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  62
agagaaacct ttatctgtat caaagaatgg tcctg                                  35


<210>  63
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  63
agaccctagc cttagataaa actgagcaag                                        30


<210>  64
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  64
tcaatgcatg ctgtttaatt gtgtggaaga                                        30


<210>  65
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  65
ttgactctat gatgaaagat gttgtt                                            26


<210>  66
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  66
attacgatcc acaaaatgta tgcag                                             25


<210>  67
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  67
agacatactt attgactcta agaggaaaga tgaagtacta tgttttaaag aatattatat       60

tacagaatta tagaaattag atctcttacc taaactcttc ataaagcttg ctctgatagg      120

aaaatgagat ctactgtttt cctttactta ctacacctca gatatatttc ttcatgaaga      180

cctcacagta aaaataggtg attttggtct agctacagtg aaatctcgat ggagtgagtc      240

ccatcagttt gaacagttgt ctggatccat tttgtggatg gtaagaattg aggctatttt      300

tccactgatt aaatttttgg ccctgagatg ctgctgagtt actagaaagt cattgaaggt      360

ctcaactata gtattttcat agttcccagt                                       390


<210>  68
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  68
agacatactt attgactcta agaggaaaga tgaagtacta tgttttaaag aatattatat       60

tacagaatta tagaaattag atctcttacc taaactcttc ataatgcttg ctctgatagg      120

aaaatgagct ctactgtttt cctttactta ctacacctca gatatatttc ttcatgaaga      180

cctcacagta aaaataggtg attttggtct agctacagag aaatctcgat ggagtgggtc      240

ccatcagttt gaacagctgt ctggatccat tttgtggatg gtaagaattg aggctatttt      300

tccactgatt aaatttttgg ccctgagatg ctgctgagtt actagaaagt cattgaaggt      360

ctcaactata gtattttcat agttcccagt                                       390


<210>  69
<211>  306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  69
ttgactctat gatgaaagat gttgttccag gatataattc actgatatgg ccatatttaa       60

acagtccaaa taatgggctg cattgcttta ttagcggatg tatagattca tagtgaccta      120

tagtaaatgc attgtactta tgtgcacatg aaatagcgct attcgaacaa atactccata      180

ctcaagatat aatgaggagc tgaaccacga cgagacataa cggaatatgc cgcattaatg      240

atcattacgc gttagacaat gtcaagatta taatcatacg tctgcataca ttttgtggat      300

cgtaat                                                                 306


<210>  70
<211>  306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  70
ttgactctat gatgaaagat gttgttccac gatatatttc tctgatatgc ccatattata       60

agagtgcaaa taatgggctc cattccttta ttagcggttg tatagtttca tagagaccaa      120

tagtaagata tctgaactta tgtccacatg taatagggct atacgaacaa atagtccata      180

cacaagatat aatgacgagg agaagcacca ccagacatat cggaatatgc cggattaatg      240

ttcattacgc cttacacaat gtcaagttta taatcatagg tctgcataca ttttgtggat      300

cgtaat                                                                 306


