
                                SEQUENCE LISTING

<110> Geneohm Sciences Canada, Inc.

<120> MOLECULAR ASSAY FOR THE AMPLIFICATION
   AND DETECTION OF KPC GENES RESPONSIBLE FOR HIGH-LEVEL
  RESISTANCE TO CARBAPENEM IN GRAM NEGATIVE BACTERIA
  

<130> 80509-534

<150> 61/565620           
<151> 2011-12-01  

<160> 32

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 1
gacacaccca tccgttac                                               18

<210> 2
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 2
ggcgttatca ctgtattgc                                              19

<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 3
aacaggcatg acggtggcgg a                                           21

<210> 4
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 4
aactgacact gggctctg                                               18

<210> 5
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 5
atacmctccg caggttcc                                               18

<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 6
acacgaccgg caaccaccgc a                                           21

<210> 7
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 7
gatrgatacc ggctcagg                                               18

<210> 8
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 8
gtaacggatg ggtgtgtc                                               18

<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 9
gctgccgctg tgctggctcg                                             20

<210> 10
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 10
cggcttgctg gacacacc                                               18

<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 11
caggccggct tgctggac                                               18

<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 12
tacggcaaaa atgcgctg                                               18

<210> 13
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 13
caacaaattg gcggcggcg                                              19

<210> 14
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 14
tatcactgta ttgcacggc                                              19

<210> 15
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 15
gcagcgaaca ggcatgacgg tggcggacgc tgc                              33

<210> 16
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 16
cgcgatcaca cgaccggcaa ccaccgcaga tcgcg                            35

<210> 17
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic oligoncuelotide

<400> 17
cgcgatcgct gccgctgtgc tggctcggat cgcg                             34

<210> 18
<211> 4244
<212> DNA
<213> Artificial Sequence

<220> 
<223> positive control vector

<400> 18
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240
gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttgacaca 300
cccatccgtt acgaaggtcg gttacaaaca gtcaccggag tagagatgtt gaaattgcag 360
gcaaattgat tgatttcacc agctgtatta gaagtacaag aaggttggtt acaacccaaa 420
gacagctgtg catgaattgc agaaaattta ttgcagcttc gccacaggaa aggtggagca 480
atgtgaagag gcatcatgcc atctgctgta ggctatcaac caatggtaag actcttctgg 540
aagcaattga gctatggtca tgccaggtga caacatatga tgagtcatgc aatacagtga 600
taacgccaag ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc 660
tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca 720
acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 780
tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 840
cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 900
gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 960
ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 1020
cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 1080
gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 1140
tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 1200
gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 1260
ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa 1320
ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca 1380
gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca 1440
gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat 1500
tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt 1560
tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag 1620
gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 1680
agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 1740
tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 1800
tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 1860
gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 1920
tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg 1980
ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 2040
cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 2100
atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 2160
gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 2220
tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 2280
gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 2340
ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 2400
atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt 2460
ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 2520
aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 2580
actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 2640
gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 2700
agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 2760
cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 2820
catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 2880
aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 2940
gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 3000
aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 3060
agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 3120
ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 3180
actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 3240
aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 3300
gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 3360
atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3420
tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3480
tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 3540
ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 3600
agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 3660
ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 3720
tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 3780
gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 3840
cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 3900
ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 3960
agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 4020
tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 4080
ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 4140
ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 4200
ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag                  4244

<210> 19
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_1

<400> 19
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 20
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_2

<400> 20
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 21
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_3

<400> 21
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 22
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_4

<400> 22
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttcggtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 23
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_5

<400> 23
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttcggtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 24
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_6

<400> 24
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 25
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_7

<400> 25
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 26
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_8

<400> 26
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 27
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_9

<400> 27
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 28
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_10

<400> 28
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttcggtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 29
<211> 180
<212> DNA
<213> Artifiical Sequence

<220> 
<223> blaKPC_11

<400> 29
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttctgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 30
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_consensus

<400> 30
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 31
<211> 180
<212> DNA
<213> Artificial Sequence

<220> 
<223> blaKPC_consensus

<400> 31
ctcgcagcca gcagcaggcc ggcttgctgg acacacccat ccgttacggc aaaaatgcgc 60
tggttccgtg gtcacccatc tcggaaaaat atctgacaac aggcatgacg gtggcggagc 120
tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc caatttgttg ctgaaggagt 180


<210> 32
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic oligonucleotide

<400> 32
cggcgatgcc tcttcacatt gctccacctt tcctcgccgg                       40
