                         SEQUENCE LISTING

<110>  Life Technologies Corporation
       HENDRICKS, Stephen
       KING, David
       XI, Lei
       PERIS, Marian
 
<120>  ENHANCED LIGATION REACTIONS

<130>  LT00621 PCT

<150>  US 61/564,243
<151>  2011-11-28

<150>  US 13/328,844
<151>  2011-12-16

<150>  PCT/US2011/065535
<151>  2011-12-16

<150>  PCT/US2012/021465
<151>  2012-01-17

<150>  US 61/692,830
<151>  2012-08-24

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of human aprataxin enzyme

<400>  1

Met Met Arg Val Cys Trp Leu Val Arg Gln Asp Ser Arg His Gln Arg 
1               5                   10                  15      


Ile Arg Leu Pro His Leu Glu Ala Val Val Ile Gly Arg Gly Pro Glu 
            20                  25                  30          


Thr Lys Ile Thr Asp Lys Lys Cys Ser Arg Gln Gln Val Gln Leu Lys 
        35                  40                  45              


Ala Glu Cys Asn Lys Gly Tyr Val Lys Val Lys Gln Val Gly Val Asn 
    50                  55                  60                  


Pro Thr Ser Ile Asp Ser Val Val Ile Gly Lys Asp Gln Glu Val Lys 
65                  70                  75                  80  


Leu Gln Pro Gly Gln Val Leu His Met Val Asn Glu Leu Tyr Pro Tyr 
                85                  90                  95      


Ile Val Glu Phe Glu Glu Glu Ala Lys Asn Pro Gly Leu Glu Thr His 
            100                 105                 110         


Arg Lys Arg Lys Arg Ser Gly Asn Ser Asp Ser Ile Glu Arg Asp Ala 
        115                 120                 125             


Ala Gln Glu Ala Glu Ala Gly Thr Gly Leu Glu Pro Gly Ser Asn Ser 
    130                 135                 140                 


Gly Gln Cys Ser Val Pro Leu Lys Lys Gly Lys Asp Ala Pro Ile Lys 
145                 150                 155                 160 


Lys Glu Ser Leu Gly His Trp Ser Gln Gly Leu Lys Ile Ser Met Gln 
                165                 170                 175     


Asp Pro Lys Met Gln Val Tyr Lys Asp Glu Gln Val Val Val Ile Lys 
            180                 185                 190         


Asp Lys Tyr Pro Lys Ala Arg Tyr His Trp Leu Val Leu Pro Trp Thr 
        195                 200                 205             


Ser Ile Ser Ser Leu Lys Ala Val Ala Arg Glu His Leu Glu Leu Leu 
    210                 215                 220                 


Lys His Met His Thr Val Gly Glu Lys Val Ile Val Asp Phe Ala Gly 
225                 230                 235                 240 


Ser Ser Lys Leu Arg Phe Arg Leu Gly Tyr His Ala Ile Pro Ser Met 
                245                 250                 255     


Ser His Val His Leu His Val Ile Ser Gln Asp Phe Asp Ser Pro Cys 
            260                 265                 270         


Leu Lys Asn Lys Lys His Trp Asn Ser Phe Asn Thr Glu Tyr Phe Leu 
        275                 280                 285             


Glu Ser Gln Ala Val Ile Glu Met Val Gln Glu Ala Gly Arg Val Thr 
    290                 295                 300                 


Val Arg Asp Gly Met Pro Glu Leu Leu Lys Leu Pro Leu Arg Cys His 
305                 310                 315                 320 


Glu Cys Gln Gln Leu Leu Pro Ser Ile Pro Gln Leu Lys Glu His Leu 
                325                 330                 335     


Arg Lys His Trp Thr Gln His His His His His His His His 
            340                 345                 350 


<210>  2
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of a human aprataxin enzyme

<400>  2
atgatgcgtg tgtgctggtt ggtgcgccag gacagccgtc accagcgtat ccgccttcca       60

catttggaag cagttgtgat tggtcgtggc ccagagacca agatcactga taagaaatgt      120

tcgcgtcagc aagttcagtt gaaagcagag tgtaacaagg gttatgtcaa ggtcaagcag      180

gtgggtgtca atccgaccag cattgacagt gtcgttattg gcaaggacca agaggtgaag      240

ctgcagccgg gccaggttct ccacatggtg aatgaacttt atccatatat tgtcgagttt      300

gaggaagagg caaagaaccc tggcctggaa acgcaccgca agcgcaagcg cagcggcaac      360

agtgattcta tcgaacgcga tgcggcgcag gaagccgagg cggggacggg cctggaacct      420

gggagcaact cgggccaatg ctctgtgccg ttaaagaagg gtaaagatgc acctatcaaa      480

aaggaatccc tgggccactg gagtcaaggc ttgaagattt ctatgcagga cccgaaaatg      540

caggtttaca aagatgagca ggtggtggtg attaaggata aatacccaaa ggcccgttac      600

cattggctgg tcttaccgtg gacctccatt tccagtctga aggcggtggc ccgtgaacac      660

cttgaactcc ttaagcatat gcacactgtg ggggaaaagg tgattgttga ttttgcgggg      720

tccagcaaac tccgcttccg tttgggctac cacgccattc cgagtatgag ccatgtgcat      780

cttcatgtga tcagccagga ttttgattct ccttgcctta aaaacaaaaa acattggaac      840

tcgttcaata cggaatactt cttagaatca caagcggtga tcgagatggt tcaagaggct      900

ggtcgcgtaa ctgtccgtga tgggatgccg gagctcttga agctgccgct tcgttgtcat      960

gagtgccagc agctgctgcc ttccattcct cagctgaaag aacatctccg caagcactgg     1020

acgcagcatc accatcacca tcaccatcac taa                                  1053


<210>  3
<211>  298
<212>  PRT
<213>  Paramecium bursaria Chlorella virus 1

<400>  3

Met Ala Ile Thr Lys Pro Leu Leu Ala Ala Thr Leu Glu Asn Ile Glu 
1               5                   10                  15      


Asp Val Gln Phe Pro Cys Leu Ala Thr Pro Lys Ile Asp Gly Ile Arg 
            20                  25                  30          


Ser Val Lys Gln Thr Gln Met Leu Ser Arg Thr Phe Lys Pro Ile Arg 
        35                  40                  45              


Asn Ser Val Met Asn Arg Leu Leu Thr Glu Leu Leu Pro Glu Gly Ser 
    50                  55                  60                  


Asp Gly Glu Ile Ser Ile Glu Gly Ala Thr Phe Gln Asp Thr Thr Ser 
65                  70                  75                  80  


Ala Val Met Thr Gly His Lys Met Tyr Asn Ala Lys Phe Ser Tyr Tyr 
                85                  90                  95      


Trp Phe Asp Tyr Val Thr Asp Asp Pro Leu Lys Lys Tyr Ile Asp Arg 
            100                 105                 110         


Val Glu Asp Met Lys Asn Tyr Ile Thr Val His Pro His Ile Leu Glu 
        115                 120                 125             


His Ala Gln Val Lys Ile Ile Pro Leu Ile Pro Val Glu Ile Asn Asn 
    130                 135                 140                 


Ile Thr Glu Leu Leu Gln Tyr Glu Arg Asp Val Leu Ser Lys Gly Phe 
145                 150                 155                 160 


Glu Gly Val Met Ile Arg Lys Pro Asp Gly Lys Tyr Lys Phe Gly Arg 
                165                 170                 175     


Ser Thr Leu Lys Glu Gly Ile Leu Leu Lys Met Lys Gln Phe Lys Asp 
            180                 185                 190         


Ala Glu Ala Thr Ile Ile Ser Met Thr Ala Leu Phe Lys Asn Thr Asn 
        195                 200                 205             


Thr Lys Thr Lys Asp Asn Phe Gly Tyr Ser Lys Arg Ser Thr His Lys 
    210                 215                 220                 


Ser Gly Lys Val Glu Glu Asp Val Met Gly Ser Ile Glu Val Asp Tyr 
225                 230                 235                 240 


Asp Gly Val Val Phe Ser Ile Gly Thr Gly Phe Asp Ala Asp Gln Arg 
                245                 250                 255     


Arg Asp Phe Trp Gln Asn Lys Glu Ser Tyr Ile Gly Lys Met Val Lys 
            260                 265                 270         


Phe Lys Tyr Phe Glu Met Gly Ser Lys Asp Cys Pro Arg Phe Pro Val 
        275                 280                 285             


Phe Ile Gly Ile Arg His Glu Glu Asp Arg 
    290                 295             


<210>  4
<211>  306
<212>  PRT
<213>  Burkholderia pseudomallei

<400>  4

Met Ser Gly Val Pro Tyr Gly Phe Lys Pro Asn Leu Ala Ala Thr Leu 
1               5                   10                  15      


Thr Lys Pro Glu Leu Ile Lys Phe Pro Val Trp Ala Ser Pro Lys Ile 
            20                  25                  30          


Asp Gly Ile Arg Cys Val Phe Phe Gly Gly Val Ala Tyr Ser Arg Ser 
        35                  40                  45              


Leu Lys Pro Ile Pro Asn Pro Val Val Gln Glu Phe Ala Lys Ala Tyr 
    50                  55                  60                  


Ala Asn Leu Leu Glu Gly Leu Asp Gly Glu Leu Thr Val Gly Ser Pro 
65                  70                  75                  80  


Thr Asp Ala Asn Cys Met Gln Asn Ser Met Ala Val Met Ser Lys Ala 
                85                  90                  95      


Ala Ala Pro Asp Phe Thr Phe His Val Phe Asp Trp Phe His Pro Ala 
            100                 105                 110         


Gln Ala His Ile Glu Phe Trp Gln Arg Ser Asp Val Val Glu Asp Arg 
        115                 120                 125             


Ile Val Gln Phe Tyr Asp Arg Tyr Pro Glu Val Asp Ile Arg Ala Ala 
    130                 135                 140                 


Pro Gln Val Leu Cys Thr Ser Leu Ala His Leu Asp Thr Asn Glu Ala 
145                 150                 155                 160 


Arg Trp Leu Ala Asp Gly Tyr Glu Gly Met Met Ile Arg Asp His Cys 
                165                 170                 175     


Gly Arg Tyr Lys Phe Gly Arg Ser Thr Glu Arg Glu Gly Gly Leu Val 
            180                 185                 190         


Lys Val Lys Arg Phe Thr Asp Ala Glu Ala Ile Val Ile Gly Phe Glu 
        195                 200                 205             


Glu Glu Met His Asn Ala Asn Glu Ala Lys Arg Asp Ala Thr Gly Arg 
    210                 215                 220                 


Thr Glu Arg Ser Thr Ser Lys Ala Gly Leu His Gly Lys Gly Thr Leu 
225                 230                 235                 240 


Gly Ala Leu Val Val Lys Asn Glu Arg Gly Ile Val Phe Asn Ile Gly 
                245                 250                 255     


Thr Gly Phe Thr Ala Ala Gln Arg Ala Asp Tyr Trp Ala Asn His Pro 
            260                 265                 270         


Ser Leu Phe Gly Lys Met Val Lys Phe Lys His Phe Asp His Gly Thr 
        275                 280                 285             


Val Asp Ala Pro Arg His Pro Val Phe Ile Gly Phe Arg His Pro Glu 
    290                 295                 300                 


Asp Met 
305     


<210>  5
<211>  268
<212>  PRT
<213>  Haemophilus influenzae

<400>  5

Met Lys Phe Tyr Arg Thr Leu Leu Leu Phe Phe Ala Ser Ser Phe Ala 
1               5                   10                  15      


Phe Ala Asn Ser Asp Leu Met Leu Leu His Thr Tyr Asn Asn Gln Pro 
            20                  25                  30          


Ile Glu Gly Trp Val Met Ser Glu Lys Leu Asp Gly Val Arg Gly Tyr 
        35                  40                  45              


Trp Asn Gly Lys Gln Leu Leu Thr Arg Gln Gly Gln Arg Leu Ser Pro 
    50                  55                  60                  


Pro Ala Tyr Phe Ile Lys Asp Phe Pro Pro Phe Ala Ile Asp Gly Glu 
65                  70                  75                  80  


Leu Phe Ser Glu Arg Asn His Phe Glu Glu Ile Ser Thr Ile Thr Lys 
                85                  90                  95      


Ser Phe Lys Gly Asp Gly Trp Glu Lys Leu Lys Leu Tyr Val Phe Asp 
            100                 105                 110         


Val Pro Asp Ala Glu Gly Asn Leu Phe Glu Arg Leu Ala Lys Leu Lys 
        115                 120                 125             


Ala His Leu Leu Glu His Pro Thr Thr Tyr Ile Glu Ile Ile Glu Gln 
    130                 135                 140                 


Ile Pro Val Lys Asp Lys Thr His Leu Tyr Gln Phe Leu Ala Gln Val 
145                 150                 155                 160 


Glu Asn Leu Gln Gly Glu Gly Val Val Val Arg Asn Pro Asn Ala Pro 
                165                 170                 175     


Tyr Glu Arg Lys Arg Ser Ser Gln Ile Leu Lys Leu Lys Thr Ala Arg 
            180                 185                 190         


Gly Glu Glu Cys Thr Val Ile Ala His His Lys Gly Lys Gly Gln Phe 
        195                 200                 205             


Glu Asn Val Met Gly Ala Leu Thr Cys Lys Asn His Arg Gly Glu Phe 
    210                 215                 220                 


Lys Ile Gly Ser Gly Phe Asn Leu Asn Glu Arg Glu Asn Pro Pro Pro 
225                 230                 235                 240 


Ile Gly Ser Val Ile Thr Tyr Lys Tyr Arg Gly Ile Thr Asn Ser Gly 
                245                 250                 255     


Lys Pro Arg Phe Ala Thr Tyr Trp Arg Glu Lys Lys 
            260                 265             


<210>  6
<211>  268
<212>  PRT
<213>  Haemophilus influenzae

<400>  6

Met Lys Phe Tyr Arg Thr Leu Leu Leu Phe Phe Ala Ser Ser Phe Ala 
1               5                   10                  15      


Phe Ala Asn Ser Asp Leu Met Leu Leu His Thr Tyr Asn Asn Gln Pro 
            20                  25                  30          


Ile Glu Gly Trp Val Met Ser Glu Lys Leu Asp Gly Val Arg Gly Tyr 
        35                  40                  45              


Trp Asn Gly Lys Gln Leu Leu Thr Arg Gln Gly Gln Arg Leu Ser Pro 
    50                  55                  60                  


Pro Ala Tyr Phe Ile Lys Asp Phe Pro Pro Phe Ala Ile Asp Gly Glu 
65                  70                  75                  80  


Leu Phe Ser Glu Arg Asn His Phe Glu Glu Ile Ser Ser Ile Thr Lys 
                85                  90                  95      


Ser Phe Lys Gly Asp Gly Trp Glu Lys Leu Lys Leu Tyr Val Phe Asp 
            100                 105                 110         


Val Pro Asp Ala Glu Gly Asn Leu Phe Glu Arg Leu Ala Lys Leu Lys 
        115                 120                 125             


Ala His Leu Leu Glu His Pro Thr Thr Tyr Ile Glu Ile Ile Glu Gln 
    130                 135                 140                 


Ile Pro Val Lys Asp Lys Thr His Leu Tyr Gln Phe Leu Ala Gln Val 
145                 150                 155                 160 


Glu Asn Leu Gln Gly Glu Gly Val Val Val Arg Asn Pro Asn Ala Pro 
                165                 170                 175     


Tyr Glu Arg Lys Arg Ser Ser Gln Ile Leu Lys Leu Lys Thr Ala Arg 
            180                 185                 190         


Gly Glu Glu Cys Thr Val Ile Ala His His Lys Gly Lys Gly Gln Phe 
        195                 200                 205             


Glu Asn Val Met Gly Ala Leu Thr Cys Lys Asn His Arg Gly Glu Phe 
    210                 215                 220                 


Lys Ile Gly Ser Gly Phe Asn Leu Asn Glu Arg Glu Asn Pro Pro Pro 
225                 230                 235                 240 


Ile Gly Ser Val Ile Thr Tyr Lys Tyr Arg Gly Ile Thr Asn Ser Gly 
                245                 250                 255     


Lys Pro Arg Phe Ala Thr Tyr Trp Arg Glu Lys Lys 
            260                 265             


<210>  7
<211>  268
<212>  PRT
<213>  Haemophilus influenzae

<400>  7

Met Lys Phe Tyr Arg Thr Leu Leu Leu Phe Phe Ala Ser Ser Phe Ala 
1               5                   10                  15      


Phe Ala Asn Ser Asp Leu Met Leu Leu His Thr Tyr Asn Asn Gln Pro 
            20                  25                  30          


Ile Glu Gly Trp Val Met Ser Glu Lys Leu Asp Gly Val Arg Gly Tyr 
        35                  40                  45              


Trp Asn Gly Lys Gln Leu Leu Thr Arg Gln Gly Gln Arg Leu Ser Pro 
    50                  55                  60                  


Pro Ala Tyr Phe Ile Lys Asp Phe Pro Pro Phe Ala Ile Asp Gly Glu 
65                  70                  75                  80  


Leu Phe Ser Glu Arg Asn His Phe Glu Glu Ile Ser Ser Ile Thr Lys 
                85                  90                  95      


Ser Phe Lys Gly Asp Gly Trp Glu Lys Leu Lys Leu Tyr Val Phe Asp 
            100                 105                 110         


Val Pro Asp Ala Glu Gly Asn Leu Phe Glu Arg Leu Ala Lys Leu Lys 
        115                 120                 125             


Ala His Leu Leu Glu His Pro Thr Thr Tyr Ile Glu Ile Ile Glu Gln 
    130                 135                 140                 


Ile Pro Val Lys Asp Lys Thr His Leu Tyr Gln Phe Leu Ala Gln Val 
145                 150                 155                 160 


Glu Asn Leu Gln Gly Glu Gly Val Val Val Arg Asn Pro Asn Ala Pro 
                165                 170                 175     


Tyr Glu Arg Lys Arg Ser Ser Gln Ile Leu Lys Leu Lys Thr Ala Arg 
            180                 185                 190         


Asp Glu Glu Cys Thr Val Ile Ala His His Lys Gly Lys Gly Gln Phe 
        195                 200                 205             


Glu Asn Val Met Gly Ala Leu Thr Cys Lys Asn His Arg Gly Glu Phe 
    210                 215                 220                 


Lys Ile Gly Ser Gly Phe Asn Leu Asn Glu Arg Glu Asn Pro Pro Pro 
225                 230                 235                 240 


Ile Gly Ser Val Ile Thr Tyr Lys Tyr Arg Gly Ile Thr Asn Ser Gly 
                245                 250                 255     


Lys Pro Arg Phe Ala Thr Tyr Trp Arg Glu Lys Lys 
            260                 265             


<210>  8
<211>  246
<212>  PRT
<213>  Haemophilus influenzae

<400>  8

Met Leu Leu His Thr Tyr Asn Asn Gln Pro Ile Glu Gly Trp Val Met 
1               5                   10                  15      


Ser Glu Lys Leu Asp Gly Val Arg Gly Tyr Trp Asn Gly Lys Gln Leu 
            20                  25                  30          


Leu Thr Arg Gln Gly Gln Arg Leu Ser Pro Pro Ala Tyr Phe Ile Lys 
        35                  40                  45              


Asp Phe Pro Pro Phe Ala Ile Asp Gly Glu Leu Phe Ser Glu Arg Asn 
    50                  55                  60                  


His Phe Glu Glu Ile Ser Ser Ile Thr Lys Ser Phe Lys Gly Asp Gly 
65                  70                  75                  80  


Trp Glu Lys Leu Lys Leu Tyr Val Phe Asp Val Pro Asp Ala Glu Gly 
                85                  90                  95      


Asn Leu Phe Glu Arg Leu Ala Lys Leu Lys Ala His Leu Leu Glu His 
            100                 105                 110         


Pro Thr Thr Tyr Ile Glu Ile Ile Glu Gln Ile Pro Val Lys Asp Lys 
        115                 120                 125             


Thr His Leu Tyr Gln Phe Leu Ala Gln Val Glu Asn Leu Gln Gly Glu 
    130                 135                 140                 


Gly Val Val Val Arg Asn Pro Asn Ala Pro Tyr Glu Arg Lys Arg Ser 
145                 150                 155                 160 


Ser Gln Ile Leu Lys Leu Lys Thr Ala Arg Asp Glu Glu Cys Thr Val 
                165                 170                 175     


Ile Ala His His Lys Gly Lys Gly Gln Phe Glu Asn Val Met Gly Ala 
            180                 185                 190         


Leu Thr Cys Lys Asn His Arg Gly Glu Phe Lys Ile Gly Ser Gly Phe 
        195                 200                 205             


Asn Leu Asn Glu Arg Glu Asn Pro Pro Pro Ile Gly Ser Val Ile Thr 
    210                 215                 220                 


Tyr Lys Tyr Arg Gly Ile Thr Asn Ser Gly Lys Pro Arg Phe Ala Thr 
225                 230                 235                 240 


Tyr Trp Arg Glu Lys Lys 
                245     


