                         SEQUENCE LISTING

<110>  The Turstees of the University of Pennsylvania
       Yan, Jian
       Weiner, David  B.
       Morrow, Matthew P.
 
<120>  COMPOSITIONS COMPRISING IMPROVED IL-12 GENETIC CONSTRUCTS AND 
       VACCINES, IMMUNOTHERAPEUTICS AND METHODS OF USING THE SAME

<130>  133172.4102

<150>  US 61/569,600
<151>  2011-12-12

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-12 Optimized p35 subunit nucleic acid

<400>  1
atgtgccccg ctcggtccct gctgctggtc gctaccctgg tcctgctgga tcacctgtca       60

ctggctcgaa atctgcctgt cgctaccccc gatcctggca tgttcccctg cctgcaccat      120

agccagaacc tgctgcgggc cgtgtccaat atgctgcaga aagctagaca gacactggag      180

ttttaccctt gtacttctga ggaaatcgac cacgaggata ttactaagga caaaacctcc      240

acagtcgaag cctgcctgcc actggagctg accaagaacg aatcatgtct gaatagcagg      300

gagacttcct tcatcaccaa cgggtcttgc ctggctagtc gcaagaccag cttcatgatg      360

gcactgtgcc tgagctccat ctacgaggat ctgaagatgt atcaggtgga attcaaaacc      420

atgaacgcta agctgctgat ggaccctaaa cgacagatct ttctggatca gaatatgctg      480

gcagtgattg acgagctgat gcaggccctg aacttcaata gcgaaaccgt cccacagaag      540

tctagtctgg aggaacccga cttttataag acaaaaatca agctgtgcat tctgctgcat      600

gcctttcgga ttcgggctgt cactattgat cgggtcatgt catacctgaa cgcttcctaa      660


<210>  2
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-12 Opt p35 subunit amino acid

<400>  2

Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val Leu Leu 
1               5                   10                  15      


Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro Asp Pro 
            20                  25                  30          


Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val 
        35                  40                  45              


Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys 
    50                  55                  60                  


Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser 
65                  70                  75                  80  


Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys 
                85                  90                  95      


Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala 
            100                 105                 110         


Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr 
        115                 120                 125             


Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys 
    130                 135                 140                 


Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu 
145                 150                 155                 160 


Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr 
                165                 170                 175     


Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys 
            180                 185                 190         


Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr 
        195                 200                 205             


Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    210                 215                 


<210>  3
<211>  987
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-12 Opt p40 subunit nucleic acid

<400>  3
atgtgccatc agcagctggt catctcttgg tttagtctgg tgtttctggc ttctccactg       60

gtcgctatct gggaactgaa aaaggatgtg tacgtggtcg agctggactg gtatccagat      120

gcacccggag aaatggtggt cctgacctgc gacacacccg aggaagatgg catcacttgg      180

accctggacc agagctccga ggtgctggga tctggcaaga cactgactat tcaggtcaaa      240

gaattcgggg atgccggaca gtacacatgt cacaagggcg gggaggtgct gagtcactca      300

ctgctgctgc tgcataagaa agaagacggc atctggtcta ctgacattct gaaggatcag      360

aaagagccta agaacaaaac cttcctgaga tgcgaagcta agaattatag tgggaggttt      420

acctgttggt ggctgaccac aatctcaact gacctgacct ttagcgtgaa atctagtagg      480

gggtcaagcg atccacaggg agtgacctgc ggagcagcta cactgagcgc cgagcgggtg      540

agaggagaca acaaggagta cgaatatagt gtcgagtgcc aggaagattc agcctgtccc      600

gcagccgagg aatccctgcc tatcgaagtg atggtggacg ctgtgcacaa gctgaaatac      660

gaaaactaca catcctcttt ctttattcgc gacatcatta agccagatcc ccctaaaaac      720

ctgcagctga agcccctgaa aaattcccga caggtggagg tctcttggga ataccctgat      780

acatggagca ctccacattc ttatttcagt ctgacttttt gcgtgcaggt ccagggcaag      840

agcaaaaggg agaagaaaga ccgcgtgttc accgataaga catccgctac tgtcatctgt      900

cgaaaaaacg caagcatttc cgtgcgggca caggataggt attattccag cagttggtct      960

gagtgggctt ccgtcccttg tagttga                                          987


<210>  4
<211>  328
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-12 Opt p40 subunit amino acid

<400>  4

Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser 
                325             


<210>  5
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE leader

<400>  5

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


