                         SEQUENCE LISTING

<110>  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE 
       SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
       Lin, Li
       Park, Sungha
       Wei, Wen
       Xiao, Rui-Ping
       Talan, Mark I.
       Edward G. Lakatta
 
<120>  HUMAN SOLUBLE RECEPTOR FOR ADVANCED GLYCATION END PRODUCTS 
       (sRAGE), METHODS OF PREPARING HUMAN sRAGE, AND TREATMENT METHODS 
       USING sRAGE

<130>  4239-90273-02

<150>  US 61/582,574
<151>  2012-01-03

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  404
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Ala Gly Thr Ala Val Gly Ala Trp Val Leu Val Leu Ser Leu 
1               5                   10                  15      


Trp Gly Ala Val Val Gly Ala Gln Asn Ile Thr Ala Arg Ile Gly Glu 
            20                  25                  30          


Pro Leu Val Leu Lys Cys Lys Gly Ala Pro Lys Lys Pro Pro Gln Arg 
        35                  40                  45              


Leu Glu Trp Lys Leu Asn Thr Gly Arg Thr Glu Ala Trp Lys Val Leu 
    50                  55                  60                  


Ser Pro Gln Gly Gly Gly Pro Trp Asp Ser Val Ala Arg Val Leu Pro 
65                  70                  75                  80  


Asn Gly Ser Leu Phe Leu Pro Ala Val Gly Ile Gln Asp Glu Gly Ile 
                85                  90                  95      


Phe Arg Cys Gln Ala Met Asn Arg Asn Gly Lys Glu Thr Lys Ser Asn 
            100                 105                 110         


Tyr Arg Val Arg Val Tyr Gln Ile Pro Gly Lys Pro Glu Ile Val Asp 
        115                 120                 125             


Ser Ala Ser Glu Leu Thr Ala Gly Val Pro Asn Lys Val Gly Thr Cys 
    130                 135                 140                 


Val Ser Glu Gly Ser Tyr Pro Ala Gly Thr Leu Ser Trp His Leu Asp 
145                 150                 155                 160 


Gly Lys Pro Leu Val Pro Asn Glu Lys Gly Val Ser Val Lys Glu Gln 
                165                 170                 175     


Thr Arg Arg His Pro Glu Thr Gly Leu Phe Thr Leu Gln Ser Glu Leu 
            180                 185                 190         


Met Val Thr Pro Ala Arg Gly Gly Asp Pro Arg Pro Thr Phe Ser Cys 
        195                 200                 205             


Ser Phe Ser Pro Gly Leu Pro Arg His Arg Ala Leu Arg Thr Ala Pro 
    210                 215                 220                 


Ile Gln Pro Arg Val Trp Glu Pro Val Pro Leu Glu Glu Val Gln Leu 
225                 230                 235                 240 


Val Val Glu Pro Glu Gly Gly Ala Val Ala Pro Gly Gly Thr Val Thr 
                245                 250                 255     


Leu Thr Cys Glu Val Pro Ala Gln Pro Ser Pro Gln Ile His Trp Met 
            260                 265                 270         


Lys Asp Gly Val Pro Leu Pro Leu Pro Pro Ser Pro Val Leu Ile Leu 
        275                 280                 285             


Pro Glu Ile Gly Pro Gln Asp Gln Gly Thr Tyr Ser Cys Val Ala Thr 
    290                 295                 300                 


His Ser Ser His Gly Pro Gln Glu Ser Arg Ala Val Ser Ile Ser Ile 
305                 310                 315                 320 


Ile Glu Pro Gly Glu Glu Gly Pro Thr Ala Gly Ser Val Gly Gly Ser 
                325                 330                 335     


Gly Leu Gly Thr Leu Ala Leu Ala Leu Gly Ile Leu Gly Gly Leu Gly 
            340                 345                 350         


Thr Ala Ala Leu Leu Ile Gly Val Ile Leu Trp Gln Arg Arg Gln Arg 
        355                 360                 365             


Arg Gly Glu Glu Arg Lys Ala Pro Glu Asn Gln Glu Glu Glu Glu Glu 
    370                 375                 380                 


Arg Ala Glu Leu Asn Gln Ser Glu Glu Pro Glu Ala Gly Glu Ser Ser 
385                 390                 395                 400 


Thr Gly Gly Pro 
                


<210>  2
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  2
atggcagccg gaacagcagt tggagcctgg gtgctggtcc tcagtctgtg gggggcagta       60

gtaggtgctg aattcatggc tagcatgact ggtggacagc aaatgggtac tggatctgct      120

caaaacatca cagcccggat tggcgagcca ctggtgctga agtgtaaggg ggcccccaag      180

aaaccacccc agcggctgga atggaaactg aacacaggcc ggacagaagc ttggaaggtc      240

ctgtctcccc agggaggagg cccctgggac agtgtggctc gtgtccttcc caacggctcc      300

ctcttccttc cggctgtcgg gatccaggat gaggggattt tccggtgcca ggcaatgaac      360

aggaatggaa aggagaccaa gtccaactac cgagtccgtg tctaccagat tcctgggaag      420

ccagaaattg tagattctgc ctctgaactc acggctggtg ttcccaataa ggtggggaca      480

tgtgtgtcag agggaagcta ccctgcaggg actcttagct ggcacttgga tgggaagccc      540

ctggtgccta atgagaaggg agtatctgtg aaggaacaga ccaggagaca ccctgagaca      600

gggctcttca cactgcagtc ggagctaatg gtgaccccag cccggggagg agatccccgt      660

cccaccttct cctgtagctt cagcccaggc cttccccgac accgggcctt gcgcacagcc      720

cccatccagc cccgtgtctg ggagcctgtg cctctggagg aggtccaatt ggtggtggag      780

ccagaaggtg gagcagtagc tcctggtgga accgtaaccc tgacctgtga agtccctgcc      840

cagccctctc ctcaaatcca ctggatgaag gatggtgtgc ccttgcccct tccccccagc      900

cctgtgctga tcctccctga gatagggcct caggaccagg gaacctacag ctgtgtggcc      960

acccattcca gccacgggcc ccaggaaagc cgtgctgtca gcatcagcat catcgaacca     1020

ggcgaggagg ggccaactgc aggctctgtg ggaggatcag ggctgggaac t              1071


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  3

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Thr 
1               5                   10          


<210>  4
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  4

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Thr Gly Ser Ala Gln 
1               5                   10                  15      


Asn Ile Thr Ala Arg Ile Gly Glu Pro Leu Val Leu Lys Cys Lys Gly 
            20                  25                  30          


Ala Pro Lys Lys Pro Pro Gln Arg Leu Glu Trp Lys Leu Asn Thr Gly 
        35                  40                  45              


Arg Thr Glu Ala Trp Lys Val Leu Ser Pro Gln Gly Gly Gly Pro Trp 
    50                  55                  60                  


Asp Ser Val Ala Arg Val Leu Pro Asn Gly Ser Leu Phe Leu Pro Ala 
65                  70                  75                  80  


Val Gly Ile Gln Asp Glu Gly Ile Phe Arg Cys Gln Ala Met Asn Arg 
                85                  90                  95      


Asn Gly Lys Glu Thr Lys Ser Asn Tyr Arg Val Arg Val Tyr Gln Ile 
            100                 105                 110         


Pro Gly Lys Pro Glu Ile Val Asp Ser Ala Ser Glu Leu Thr Ala Gly 
        115                 120                 125             


Val Pro Asn Lys Val Gly Thr Cys Val Ser Glu Gly Ser Tyr Pro Ala 
    130                 135                 140                 


Gly Thr Leu Ser Trp His Leu Asp Gly Lys Pro Leu Val Pro Asn Glu 
145                 150                 155                 160 


Lys Gly Val Ser Val Lys Glu Gln Thr Arg Arg His Pro Glu Thr Gly 
                165                 170                 175     


Leu Phe Thr Leu Gln Ser Glu Leu Met Val Thr Pro Ala Arg Gly Gly 
            180                 185                 190         


Asp Pro Arg Pro Thr Phe Ser Cys Ser Phe Ser Pro Gly Leu Pro Arg 
        195                 200                 205             


His Arg Ala Leu Arg Thr Ala Pro Ile Gln Pro Arg Val Trp Glu Pro 
    210                 215                 220                 


Val Pro Leu Glu Glu Val Gln Leu Val Val Glu Pro Glu Gly Gly Ala 
225                 230                 235                 240 


Val Ala Pro Gly Gly Thr Val Thr Leu Thr Cys Glu Val Pro Ala Gln 
                245                 250                 255     


Pro Ser Pro Gln Ile His Trp Met Lys Asp Gly Val Pro Leu Pro Leu 
            260                 265                 270         


Pro Pro Ser Pro Val Leu Ile Leu Pro Glu Ile Gly Pro Gln Asp Gln 
        275                 280                 285             


Gly Thr Tyr Ser Cys Val Ala Thr His Ser Ser His Gly Pro Gln Glu 
    290                 295                 300                 


Ser Arg Ala Val Ser Ile Ser Ile Ile Glu Pro Gly Glu Glu Gly Pro 
305                 310                 315                 320 


Thr Ala Gly Ser Val Gly Gly Ser Gly Leu Gly Thr 
                325                 330         


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  5
gaagatctgc tcaaaccatc acagcccgga ttggc                                  35


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  6
gggaaggaca cgagccac                                                     18


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  7
accggctccc tcttccttcc g                                                 21


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  8
gctctagatc aagttcccag ccctg                                             25


<210>  9
<211>  1035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  CDS
<222>  (1)..(1035)

<400>  9
atg gct agc atg act ggt gga cag caa atg ggt act ctg gtg ccc cgg         48
Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Thr Leu Val Pro Arg           
1               5                   10                  15                

gga tct ggc gac gga tct ggc ggc ggc agc ggc ggc caa aac atc aca         96
Gly Ser Gly Asp Gly Ser Gly Gly Gly Ser Gly Gly Gln Asn Ile Thr           
            20                  25                  30                    

gcc cgg att ggc gag cca ctg gtg ctg aag tgt aag ggg gcc ccc aag        144
Ala Arg Ile Gly Glu Pro Leu Val Leu Lys Cys Lys Gly Ala Pro Lys           
        35                  40                  45                        

aaa cca ccc cag cgg ctg gaa tgg aaa ctg aac aca ggc cgg aca gaa        192
Lys Pro Pro Gln Arg Leu Glu Trp Lys Leu Asn Thr Gly Arg Thr Glu           
    50                  55                  60                            

gct tgg aag gtc ctg tct ccc cag gga gga ggc ccc tgg gac agt gtg        240
Ala Trp Lys Val Leu Ser Pro Gln Gly Gly Gly Pro Trp Asp Ser Val           
65                  70                  75                  80            

gct cgt gtc ctt ccc aac ggc tcc ctc ttc ctt ccg gct gtc ggg atc        288
Ala Arg Val Leu Pro Asn Gly Ser Leu Phe Leu Pro Ala Val Gly Ile           
                85                  90                  95                

cag gat gag ggg att ttc cgg tgc cag gca atg aac agg aat gga aag        336
Gln Asp Glu Gly Ile Phe Arg Cys Gln Ala Met Asn Arg Asn Gly Lys           
            100                 105                 110                   

gag acc aag tcc aac tac cga gtc cgt gtc tac cag att cct ggg aag        384
Glu Thr Lys Ser Asn Tyr Arg Val Arg Val Tyr Gln Ile Pro Gly Lys           
        115                 120                 125                       

cca gaa att gta gat tct gcc tct gaa ctc acg gct ggt gtt ccc aat        432
Pro Glu Ile Val Asp Ser Ala Ser Glu Leu Thr Ala Gly Val Pro Asn           
    130                 135                 140                           

aag gtg ggg aca tgt gtg tca gag gga agc tac cct gca ggg act ctt        480
Lys Val Gly Thr Cys Val Ser Glu Gly Ser Tyr Pro Ala Gly Thr Leu           
145                 150                 155                 160           

agc tgg cac ttg gat ggg aag ccc ctg gtg cct aat gag aag gga gta        528
Ser Trp His Leu Asp Gly Lys Pro Leu Val Pro Asn Glu Lys Gly Val           
                165                 170                 175               

tct gtg aag gaa cag acc agg aga cac cct gag aca ggg ctc ttc aca        576
Ser Val Lys Glu Gln Thr Arg Arg His Pro Glu Thr Gly Leu Phe Thr           
            180                 185                 190                   

ctg cag tcg gag cta atg gtg acc cca gcc cgg gga gga gat ccc cgt        624
Leu Gln Ser Glu Leu Met Val Thr Pro Ala Arg Gly Gly Asp Pro Arg           
        195                 200                 205                       

ccc acc ttc tcc tgt agc ttc agc cca ggc ctt ccc cga cac cgg gcc        672
Pro Thr Phe Ser Cys Ser Phe Ser Pro Gly Leu Pro Arg His Arg Ala           
    210                 215                 220                           

ttg cgc aca gcc ccc atc cag ccc cgt gtc tgg gag cct gtg cct ctg        720
Leu Arg Thr Ala Pro Ile Gln Pro Arg Val Trp Glu Pro Val Pro Leu           
225                 230                 235                 240           

gag gag gtc caa ttg gtg gtg gag cca gaa ggt gga gca gta gct cct        768
Glu Glu Val Gln Leu Val Val Glu Pro Glu Gly Gly Ala Val Ala Pro           
                245                 250                 255               

ggt gga acc gta acc ctg acc tgt gaa gtc cct gcc cag ccc tct cct        816
Gly Gly Thr Val Thr Leu Thr Cys Glu Val Pro Ala Gln Pro Ser Pro           
            260                 265                 270                   

caa atc cac tgg atg aag gat ggt gtg ccc ttg ccc ctt ccc ccc agc        864
Gln Ile His Trp Met Lys Asp Gly Val Pro Leu Pro Leu Pro Pro Ser           
        275                 280                 285                       

cct gtg ctg atc ctc cct gag ata ggg cct cag gac cag gga acc tac        912
Pro Val Leu Ile Leu Pro Glu Ile Gly Pro Gln Asp Gln Gly Thr Tyr           
    290                 295                 300                           

agc tgt gtg gcc acc cat tcc agc cac ggg ccc cag gaa agc cgt gct        960
Ser Cys Val Ala Thr His Ser Ser His Gly Pro Gln Glu Ser Arg Ala           
305                 310                 315                 320           

gtc agc atc agc atc atc gaa cca ggc gag gag ggg cca act gca ggc       1008
Val Ser Ile Ser Ile Ile Glu Pro Gly Glu Glu Gly Pro Thr Ala Gly           
                325                 330                 335               

tct gtg gga gga tca ggg ctg gga act                                   1035
Ser Val Gly Gly Ser Gly Leu Gly Thr                                       
            340                 345                                       


<210>  10
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Thr Leu Val Pro Arg 
1               5                   10                  15      


Gly Ser Gly Asp Gly Ser Gly Gly Gly Ser Gly Gly Gln Asn Ile Thr 
            20                  25                  30          


Ala Arg Ile Gly Glu Pro Leu Val Leu Lys Cys Lys Gly Ala Pro Lys 
        35                  40                  45              


Lys Pro Pro Gln Arg Leu Glu Trp Lys Leu Asn Thr Gly Arg Thr Glu 
    50                  55                  60                  


Ala Trp Lys Val Leu Ser Pro Gln Gly Gly Gly Pro Trp Asp Ser Val 
65                  70                  75                  80  


Ala Arg Val Leu Pro Asn Gly Ser Leu Phe Leu Pro Ala Val Gly Ile 
                85                  90                  95      


Gln Asp Glu Gly Ile Phe Arg Cys Gln Ala Met Asn Arg Asn Gly Lys 
            100                 105                 110         


Glu Thr Lys Ser Asn Tyr Arg Val Arg Val Tyr Gln Ile Pro Gly Lys 
        115                 120                 125             


Pro Glu Ile Val Asp Ser Ala Ser Glu Leu Thr Ala Gly Val Pro Asn 
    130                 135                 140                 


Lys Val Gly Thr Cys Val Ser Glu Gly Ser Tyr Pro Ala Gly Thr Leu 
145                 150                 155                 160 


Ser Trp His Leu Asp Gly Lys Pro Leu Val Pro Asn Glu Lys Gly Val 
                165                 170                 175     


Ser Val Lys Glu Gln Thr Arg Arg His Pro Glu Thr Gly Leu Phe Thr 
            180                 185                 190         


Leu Gln Ser Glu Leu Met Val Thr Pro Ala Arg Gly Gly Asp Pro Arg 
        195                 200                 205             


Pro Thr Phe Ser Cys Ser Phe Ser Pro Gly Leu Pro Arg His Arg Ala 
    210                 215                 220                 


Leu Arg Thr Ala Pro Ile Gln Pro Arg Val Trp Glu Pro Val Pro Leu 
225                 230                 235                 240 


Glu Glu Val Gln Leu Val Val Glu Pro Glu Gly Gly Ala Val Ala Pro 
                245                 250                 255     


Gly Gly Thr Val Thr Leu Thr Cys Glu Val Pro Ala Gln Pro Ser Pro 
            260                 265                 270         


Gln Ile His Trp Met Lys Asp Gly Val Pro Leu Pro Leu Pro Pro Ser 
        275                 280                 285             


Pro Val Leu Ile Leu Pro Glu Ile Gly Pro Gln Asp Gln Gly Thr Tyr 
    290                 295                 300                 


Ser Cys Val Ala Thr His Ser Ser His Gly Pro Gln Glu Ser Arg Ala 
305                 310                 315                 320 


Val Ser Ile Ser Ile Ile Glu Pro Gly Glu Glu Gly Pro Thr Ala Gly 
                325                 330                 335     


Ser Val Gly Gly Ser Gly Leu Gly Thr 
            340                 345 


<210>  11
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  11

Gly Ser Gly Asp Gly Ser Gly Gly Gly Ser Gly Gly Gln Asn Ile Thr 
1               5                   10                  15      


Ala Arg Ile Gly Glu Pro Leu Val Leu Lys Cys Lys Gly Ala Pro Lys 
            20                  25                  30          


Lys Pro Pro Gln Arg Leu Glu Trp Lys Leu Asn Thr Gly Arg Thr Glu 
        35                  40                  45              


Ala Trp Lys Val Leu Ser Pro Gln Gly Gly Gly Pro Trp Asp Ser Val 
    50                  55                  60                  


Ala Arg Val Leu Pro Asn Gly Ser Leu Phe Leu Pro Ala Val Gly Ile 
65                  70                  75                  80  


Gln Asp Glu Gly Ile Phe Arg Cys Gln Ala Met Asn Arg Asn Gly Lys 
                85                  90                  95      


Glu Thr Lys Ser Asn Tyr Arg Val Arg Val Tyr Gln Ile Pro Gly Lys 
            100                 105                 110         


Pro Glu Ile Val Asp Ser Ala Ser Glu Leu Thr Ala Gly Val Pro Asn 
        115                 120                 125             


Lys Val Gly Thr Cys Val Ser Glu Gly Ser Tyr Pro Ala Gly Thr Leu 
    130                 135                 140                 


Ser Trp His Leu Asp Gly Lys Pro Leu Val Pro Asn Glu Lys Gly Val 
145                 150                 155                 160 


Ser Val Lys Glu Gln Thr Arg Arg His Pro Glu Thr Gly Leu Phe Thr 
                165                 170                 175     


Leu Gln Ser Glu Leu Met Val Thr Pro Ala Arg Gly Gly Asp Pro Arg 
            180                 185                 190         


Pro Thr Phe Ser Cys Ser Phe Ser Pro Gly Leu Pro Arg His Arg Ala 
        195                 200                 205             


Leu Arg Thr Ala Pro Ile Gln Pro Arg Val Trp Glu Pro Val Pro Leu 
    210                 215                 220                 


Glu Glu Val Gln Leu Val Val Glu Pro Glu Gly Gly Ala Val Ala Pro 
225                 230                 235                 240 


Gly Gly Thr Val Thr Leu Thr Cys Glu Val Pro Ala Gln Pro Ser Pro 
                245                 250                 255     


Gln Ile His Trp Met Lys Asp Gly Val Pro Leu Pro Leu Pro Pro Ser 
            260                 265                 270         


Pro Val Leu Ile Leu Pro Glu Ile Gly Pro Gln Asp Gln Gly Thr Tyr 
        275                 280                 285             


Ser Cys Val Ala Thr His Ser Ser His Gly Pro Gln Glu Ser Arg Ala 
    290                 295                 300                 


Val Ser Ile Ser Ile Ile Glu Pro Gly Glu Glu Gly Pro Thr Ala Gly 
305                 310                 315                 320 


Ser Val Gly Gly Ser Gly Leu Gly Thr 
                325                 


<210>  12
<211>  1065
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  12
atggctgctg gcaccgctgt gggagcttgg gtgctggtgc tgtccctgtg gggcgctgtc       60

gtgggcgccg agttcatggc ctctatgacc ggcggacagc agatgggcac ccagaacatc      120

accgcccgga tcggagagcc cctggtcctg aagtgcaagg gcgctcccaa gaagcccccc      180

cagcggctgg aatggaagct gaacaccggc cggaccgagg cctggaaggt gctgagtcct      240

cagggcggag gcccttggga ctccgtggcc agagtgctgc ccaacggctc cctgtttctg      300

cccgccgtgg gcatccagga cgagggcatc ttcaggtgtc aggccatgaa ccggaacggc      360

aaagagacaa agtccaacta cagagtgcgg gtgtaccaga tccccggcaa gcccgagatc      420

gtggactccg cctctgagct gaccgctggc gtgcccaaca aagtgggcac ctgtgtgtcc      480

gagggctcct accctgccgg caccctgtct tggcacctgg acggcaagcc tctggtgccc      540

aacgagaagg gcgtgtccgt gaaagagcag accagacggc accccgagac aggcctgttc      600

accctgcagt ccgagctgat ggtcaccccc gccagaggcg gcgaccccag acctaccttc      660

agctgctcct tctcccctgg cctgccccgg cacagagccc tgagaaccgc ccccatccag      720

cccagagtgt gggagcccgt gcccctggaa gaggtgcagc tggtggtgga acctgagggc      780

ggagctgtgg ctcctggcgg caccgtgacc ctgacctgtg aagtgcctgc ccagcccagc      840

ccccagatcc actggatgaa ggacggcgtg cccctgcccc tgcctccctc tcctgtgctg      900

atcctgcctg agatcggccc ccaggaccag ggcacctact cttgcgtggc cacccactcc      960

tcccacggcc ctcaggaatc tcgggccgtc agcatctcca tcatcgagcc cggcgaggaa     1020

ggccctaccg ctggctctgt gggcggctct ggcctgggca cctga                     1065


