                         SEQUENCE LISTING

<110>  MEADOR, James W
 
<120>  Nucleotide Cloning Methods

<130>  2010403-0015 (M0082 PCT)

<140>  PCT/US2013/021099
<141>  2013-01-11

<150>  61/586,317
<151>  2012-01-13

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type-IIS restriction enzyme Sap1 5'-3' sense strand recogniton 
       site


<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Sap1 sense stand cleavage site

<220>
<221>  misc_feature
<222>  (8)..(12)
<223>  A, G, T or C

<400>  1
gctcttcnnn nn                                                           12


<210>  2
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type-IIS restriction enzyme Sap1 5'-3' anti-sense strand 
       recogniton site


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Sap1 anti-sense stand cleavage site

<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  A, G, T or C

<400>  2
nnnnngaaga gc                                                           12


<210>  3
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  first SapI site on sense strand in Mannosidase 2


<220>
<221>  misc_feature
<222>  (8)..(14)
<223>  Sense strand SapI recognition site

<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  resulting 5' overhang

<400>  3
aagtttcgct cttctacaga agctccggct aagctga                                37


<210>  4
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  first SapI site on anti-sense strand in Mannosidase 2


<220>
<221>  misc_feature
<222>  (20)..(22)
<223>  resulting 3' overhang

<220>
<221>  misc_feature
<222>  (24)..(30)
<223>  Anti-sense strand Sap1 recognition site

<400>  4
tcagcttagc cggagcttct gtagaagagc gaaactt                                37


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second SapI site on sense strand in Mannosidase 2


<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  resulting 5' overhang

<220>
<221>  misc_feature
<222>  (21)..(27)
<223>  Sense strand SapI recognition site

<400>  5
gacaaggctg ctatagccac gaagagcgat tgttgg                                 36


<210>  6
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second SapI site on anti-sense strand in Mannosidase 2


<220>
<221>  misc_feature
<222>  (10)..(16)
<223>  Anti-sense strand SapI recognition site

<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  resulting 3' overhang

<400>  6
ccaacaatcg ctcttcgtgg ctatagcagc cttgtc                                 36


<210>  7
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized oligonucleotide primer

<400>  7
aaccgctctt ccatgtttta tcaactggaa aagctgca                               38


<210>  8
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(47)
<223>  sense strand

<400>  8
ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactc                     47


<210>  9
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(47)
<223>  anti-sense strand

<400>  9
gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcc                     47


<210>  10
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(39)
<223>  sense strand primer

<400>  10
aattgctctt cacttttccg cttcctcgct cactgactc                              39


<210>  11
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  anti-sense strand primer

<400>  11
ttaagctctt caaagcgccc aatacgcaaa ccgcc                                  35


