                         SEQUENCE LISTING

<110>  GREEN, Roland
       GLASER, Bryan
       MEYVANTSSON, Ivar
       KAUFMAN, Kimberly
       GREEN, Madison
 
<120>  HIGH THROUGHPUT SCREEN FOR BIOLOGICALLY ACTIVE POLYPEPTIDES

<130>  INVR.P0002WO

<140>  Unknown
<141>  2013-02-01

<150>  61/594,149
<151>  2012-02-02

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  PRT
<213>  Apis mellifera

<400>  1

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 
            20                  25      


<210>  2
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  2

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Thr Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Ser Lys Arg Gln Lys 
            20                  25      


<210>  3
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  3

Gly Ile Gly Ala Leu Ile Lys Val Leu Thr Thr Gly Leu Pro Met Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Asn Lys 
            20                  25      


<210>  4
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  4

Gly Ile Gly Ala Trp Thr Lys Val Leu Thr Thr Gly Leu Pro Gly Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Leu Arg 
            20                  25      


<210>  5
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  5

Gly Ile Gly Ala Thr Val Lys Val Leu Ser Thr Gly Leu Arg Phe Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Lys Tyr 
            20                  25      


<210>  6
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  6

Gly Ile Gly Ala Ile Ala Lys Val Leu Ser Thr Gly Leu Pro Arg Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Gly Lys Arg Ile Arg 
            20                  25      


<210>  7
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  7

Gly Ile Gly Ala Val Leu Lys Val Leu Gly Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Phe Lys Arg Phe Pro 
            20                  25      


<210>  8
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  8

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Val Arg 
1               5                   10                  15      


Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala 
            20                  25                  30          


Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn 
        35                  40                  45              


Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val 
    50                  55                  60                  


Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly 
65                  70                  75                  80  


Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg 
                85                  90                  95      


Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys 
            100                 105                 110         


Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp 
        115                 120                 125             


Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp 
    130                 135                 140                 


Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu 
145                 150                 155                 160 


Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
                165                 170     


<210>  9
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  9

Met His His His His His His Gly Ser Gly Gly Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro 
            20                  25                  30          


Val Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp 
        35                  40                  45              


Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg 
    50                  55                  60                  


Asp Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser 
65                  70                  75                  80  


Gln Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu 
                85                  90                  95      


Thr His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn 
            100                 105                 110         


Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly 
        115                 120                 125             


Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe 
    130                 135                 140                 


Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp 
145                 150                 155                 160 


Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala 
                165                 170                 175     


Leu 
    


<210>  10
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  10

Met His His His His His His Gly Ser Gly Gly Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro 
            20                  25                  30          


Val Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp 
        35                  40                  45              


Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg 
    50                  55                  60                  


Asp Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser 
65                  70                  75                  80  


Gln Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu 
                85                  90                  95      


Thr His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn 
            100                 105                 110         


Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly 
        115                 120                 125             


Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe 
    130                 135                 140                 


Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp 
145                 150                 155                 160 


Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala 
                165                 170                 175     


Leu 
    


<210>  11
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  11

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Ser Ala 
1               5                   10                  15      


Gly Gln Ser Ser Gly Arg Ala Thr Ala Leu Thr Glu Gly Ala Lys Leu 
            20                  25                  30          


Phe Glu Lys Glu Ile Pro Tyr Ile Thr Glu Leu Glu Gly Asp Val Glu 
        35                  40                  45              


Gly Met Lys Phe Ile Ile Lys Gly Glu Gly Thr Gly Asp Ala Thr Thr 
    50                  55                  60                  


Gly Thr Ile Lys Ala Lys Tyr Ile Cys Thr Thr Gly Asp Leu Pro Val 
65                  70                  75                  80  


Pro Trp Ala Thr Leu Val Ser Thr Leu Ser Tyr Gly Val Gln Cys Phe 
                85                  90                  95      


Ala Lys Tyr Pro Ser His Ile Lys Asp Phe Phe Lys Ser Ala Met Pro 
            100                 105                 110         


Glu Gly Tyr Thr Gln Glu Arg Thr Ile Ser Phe Glu Gly Asp Gly Val 
        115                 120                 125             


Tyr Lys Thr Arg Ala Met Val Thr Tyr Glu Arg Gly Ser Ile Tyr Asn 
    130                 135                 140                 


Arg Val Thr Leu Thr Gly Glu Asn Phe Lys Lys Asp Gly His Ile Leu 
145                 150                 155                 160 


Arg Lys Asn Val Ala Phe Gln Cys Pro Pro Ser Ile Leu Tyr Ile Leu 
                165                 170                 175     


Pro Asp Thr Val Asn Asn Gly Ile Arg Val Glu Phe Asn Gln Ala Tyr 
            180                 185                 190         


Asp Ile Glu Gly Val Thr Glu Lys Leu Val Thr Lys Cys Ser Gln Met 
        195                 200                 205             


Asn Arg Pro Leu Ala Gly Ser Ala Ala Val His Ile Pro Arg Tyr His 
    210                 215                 220                 


His Ile Thr Tyr His Thr Lys Leu Ser Lys Asp Arg Asp Glu Arg Arg 
225                 230                 235                 240 


Asp His Met Cys Leu Val Glu Val Val Lys Ala Val Asp Leu Asp Thr 
                245                 250                 255     


Tyr Gln Ala Gly Ala Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
            260                 265                 270         


<210>  12
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  12

Met His His His His His His Gly Ser Gly Gly Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Gly Ser Gly Gly Ser Gly Asp Ile Gln Met Thr Gln 
            20                  25                  30          


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
        35                  40                  45              


Cys Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp Tyr Gln Gln 
    50                  55                  60                  


Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile Tyr Ser Ala Ser Phe Leu 
65                  70                  75                  80  


Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu Thr Phe Gly Gln Gly Thr 
        115                 120                 125             


Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser 
145                 150                 155                 160 


Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala 
                165                 170                 175     


Ala Ser Gly Tyr Val Phe Thr Asp Tyr Gly Met Asn Trp Val Arg Gln 
            180                 185                 190         


Ala Pro Gly Lys Gly Leu Glu Trp Met Gly Trp Ile Asn Thr Tyr Ile 
        195                 200                 205             


Gly Glu Pro Ile Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Phe Ser 
    210                 215                 220                 


Leu Asp Thr Ser Lys Ser Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg 
225                 230                 235                 240 


Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Tyr Arg Ser Tyr 
                245                 250                 255     


Ala Met Asp Tyr Trp Gln Gln Gly Thr Leu Val Thr Val Ser Ser 
            260                 265                 270     


<210>  13
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  13

Met His His His His His His Gly Ser Gly Gly Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Asp Phe Lys Cys Pro Ser Glu Trp Tyr Ala Tyr Asp 
            20                  25                  30          


Gln His Cys Tyr Arg Ile Ile Asn 
        35                  40  


<210>  14
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  14

Met His His His His His His Gly Ser Gly Gly Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Gly Ser Gly Gly Ser Gly Val Arg Ser Ser Ser Arg 
            20                  25                  30          


Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala 
        35                  40                  45              


Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala 
    50                  55                  60                  


Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly 
65                  70                  75                  80  


Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
                85                  90                  95      


Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Ser 
            100                 105                 110         


Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln 
        115                 120                 125             


Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile 
    130                 135                 140                 


Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala 
145                 150                 155                 160 


Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val 
                165                 170                 175     


Tyr Phe Gly Ile Ile Ala Leu 
            180             


<210>  15
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  15

Met His His His His His His Gly Ser Gly Gly Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Gly Ser Gly Gly Ser Gly Asp Ile Gln Met Thr Gln 
            20                  25                  30          


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
        35                  40                  45              


Cys Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp Tyr Gln Gln 
    50                  55                  60                  


Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile Tyr Ser Ala Ser Phe Leu 
65                  70                  75                  80  


Tyr Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Gln Gln Tyr Asn Ile Tyr Pro Leu Thr Phe Gly Gln Gly Thr 
        115                 120                 125             


Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser 
145                 150                 155                 160 


Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala 
                165                 170                 175     


Ala Ser Gly Tyr Val Phe Thr Asp Tyr Gly Met Asn Trp Val Arg Gln 
            180                 185                 190         


Ala Pro Gly Lys Gly Leu Glu Trp Met Gly Trp Ile Asn Thr Tyr Ile 
        195                 200                 205             


Gly Glu Pro Ile Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Phe Ser 
    210                 215                 220                 


Leu Asp Thr Ser Lys Ser Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg 
225                 230                 235                 240 


Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Tyr Arg Ser Tyr 
                245                 250                 255     


Ala Met Asp Tyr Trp Gln Gln Gly Thr Leu Val Thr Val Ser Ser 
            260                 265                 270     


<210>  16
<211>  20
<212>  PRT
<213>  Apis mellifera

<400>  16

Glu Val Lys His Met His His His His His His Glu Asn Leu Tyr Phe 
1               5                   10                  15      


Gln Gly Gly Ile 
            20  


<210>  17
<211>  60
<212>  DNA
<213>  Apis mellifera

<400>  17
gaggtaaaac atatgcatca ccaccaccat cacgagaatc tgtagtttca aggcggtatc       60


<210>  18
<211>  20
<212>  PRT
<213>  Apis mellifera

<400>  18

Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu Ile Ser 
1               5                   10                  15      


Trp Ile Lys Arg 
            20  


<210>  19
<211>  60
<212>  DNA
<213>  Apis mellifera

<400>  19
ggcgcagttc tgaaagtgct gaccacgggt ttgccggctc tgattagctg gatcaaacgt       60


<210>  20
<211>  6
<212>  PRT
<213>  Apis mellifera

<400>  20

Lys Arg Gln Gln Leu Glu 
1               5       


<210>  21
<211>  24
<212>  DNA
<213>  Apis mellifera

<400>  21
aagcgccasc agtgataact cgag                                              24


<210>  22
<211>  7
<212>  PRT
<213>  Apis mellifera

<400>  22

Val Leu Thr Pro Ala Gln Gln 
1               5           


<210>  23
<211>  24
<212>  DNA
<213>  Apis mellifera

<400>  23
gttctgaccc cggctcgtca gcag                                              24


<210>  24
<211>  26
<212>  PRT
<213>  Apis mellifera

<400>  24

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 
            20                  25      


<210>  25
<211>  26
<212>  PRT
<213>  Apis mellifera


<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  X is either Pro or Arg

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Xaa can be any naturally occurring amino acid

<400>  25

Gly Ile Gly Ala Xaa Xaa Lys Val Leu Xaa Thr Gly Leu Cys Cys Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Xaa Lys Arg Lys Lys 
            20                  25      


<210>  26
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  26

His His His His His His 
1               5       


