                         SEQUENCE LISTING

<110>  Wisconsin Alumni Research Foundation
       Wu, Cheng-Hsien
       Smith, Lloyd L.
 
<120>  RNA-MEDIATED GENE ASSEMBLY FROM DNA OLIGONUCLEOTIDES

<130>  960296.01507

<150>  61/597,428
<151>  2012-02-10

<160>  78    

<170>  PatentIn version 3.5

<210>  1
<211>  706
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence (ZsGreen1, adapted from Clontech's pZsGreen1-C1 
       vector)

<400>  1
ggtcgccacc atggcccagt ccaagcacgg cctgaccaag gagatgacca tgaagtaccg       60

catggagggc tgcgtggacg gccacaagtt cgtgatcacc ggcgagggca tcggctaccc      120

cttcaagggc aagcaggcca tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc      180

cgaggacatc ttgtccgccg ccttcatgta cggcaaccgc gtgttcaccg agtaccccca      240

ggacatcgtc gactacttca agaactcctg ccccgccggc tacacctggg accgctcctt      300

cctgttcgag gacggcgccg tgtgcatctg caacgccgac atcaccgtga gcgtggagga      360

gaactgcatg taccacgagt ccaagttcta cggcgtgaac ttccccgccg acggccccgt      420

gatgaagaag atgaccgaca actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa      480

gcagggcatc ttgaagggcg acgtgagcat gtacctgctg ctgaaggacg gtggccgctt      540

gcgctgccag ttcgacaccg tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg      600

gcacttcatc cagcacaagc tgacccgcga ggaccgcagc gacgccaaga accagaagtg      660

gcacctgacc gagcacgcca tcgcctccgg ctccgccttg ccctga                     706


<210>  2
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #1 Target segment RNAs for ZsGreen1 assembly (5' to 3')

<400>  2
ggucgccacc auggcccagu ccaagcacgg ccugaccaa                              39


<210>  3
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #2 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  3
ggagaugacc augaaguacc gcauggaggg cugcgu                                 36


<210>  4
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #3 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  4
ggacggccac aaguucguga ucaccggcga                                        30


<210>  5
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #4 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  5
gggcaucggc uaccccuuca agggcaagca                                        30


<210>  6
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #5 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  6
ggccaucaac cugugcgugg uggagggcgg ccccuugccc uucgccga                    48


<210>  7
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #6 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  7
ggacaucuug uccgccgccu ucauguacgg caaccgcgug uucaccgagu accccca          57


<210>  8
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #7 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  8
ggacaucguc gacuacuuca agaacuccug ccccgcc                                37


<210>  9
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #8 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  9
ggcuacaccu gggaccgcuc cuuccuguuc ga                                     32


<210>  10
<211>  45
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #9 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  10
ggacggcgcc gugugcaucu gcaacgccga caucaccgug agcgu                       45


<210>  11
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #10 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  11
ggaggagaac ugcauguacc acgaguccaa guucuac                                37


<210>  12
<211>  52
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #11 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  12
ggcgugaacu uccccgccga cggccccgug augaagaaga ugaccgacaa cu               52


<210>  13
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #12 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  13
gggagcccuc cugcgagaag aucauccccg ugcccaagca                             40


<210>  14
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #13 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  14
gggcaucuug aagggcgacg ugagcaugua ccugcugcug aa                          42


<210>  15
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #14 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  15
ggacgguggc cgcuugcgcu gccaguucga caccguguac aa                          42


<210>  16
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #15 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  16
ggccaagucc gugccccgca agaugcccga cu                                     32


<210>  17
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #16 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  17
ggcacuucau ccagcacaag cugacccgcg a                                      31


<210>  18
<211>  58
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #17 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  18
ggaccgcagc gacgccaaga accagaagug gcaccugacc gagcacgcca ucgccucc         58


<210>  19
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #18 Target segment-RNAs for ZsGreen1 assembly (5'to 3')

<400>  19
ggcuccgccu ugcccuga                                                     18


<210>  20
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #1 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  20
gggcacggcc ugaccaagga gaugaccaug aa                                     32


<210>  21
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #2 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  21
ggcauggagg gcugcgugga cggccacaag uu                                     32


<210>  22
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #3 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  22
ggcgugauca ccggcgaggg caucggcuac cc                                     32


<210>  23
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #4 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  23
ggcuucaagg gcaagcaggc caucaaccug ug                                     32


<210>  24
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #5 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  24
ggcuugcccu ucgccgagga caucuugucc gc                                     32


<210>  25
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #6 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  25
ggcaccgagu acccccagga caucgucgac ua                                     32


<210>  26
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #7 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  26
ggaacuccug ccccgccggc uacaccuggg ac                                     32


<210>  27
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #8 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  27
ggcuccuucc uguucgagga cggcgccgug ug                                     32


<210>  28
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #9 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  28
ggcaucaccg ugagcgugga ggagaacugc au                                     32


<210>  29
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #10 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  29
gggaguccaa guucuacggc gugaacuucc cc                                     32


<210>  30
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #11 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  30
ggagaugacc gacaacuggg agcccuccug cg                                     32


<210>  31
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #12 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  31
ggccccgugc ccaagcaggg caucuugaag gg                                     32


<210>  32
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #13 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  32
ggguaccugc ugcugaagga cgguggccgc uu                                     32


<210>  33
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #14 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  33
ggcgacaccg uguacaaggc caaguccgug cc                                     32


<210>  34
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #15 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  34
gggcaagaug cccgacuggc acuucaucca gc                                     32


<210>  35
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #16 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  35
ggcaagcuga cccgcgagga ccgcagcgac gc                                     32


<210>  36
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  #17 Target splint-RNAs for ZsGreen1 assembly (5'to 3')

<400>  36
ggcacgccau cgccuccggc uccgccuugc cc                                     32


<210>  37
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZsG-F RT-PCR primer

<400>  37
atggcccagt ccaagcac                                                     18


<210>  38
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZsG-R-w-6His RT-PCR Primer

<400>  38
ctagtggtga tggtgatgat ggggcaaggc ggagc                                  35


<210>  39
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7P-ZsG-F RT-PCR primer

<400>  39
cagtaatacg actcactata ggtcgccacc atggcccagt ccaagcacg                   49


<210>  40
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZsG-R RT-PCR primer

<400>  40
tcagggcaag gcggagc                                                      17


<210>  41
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FAM(6-carboxyfluorescein) RT-PCR primer

<400>  41
cagtaatacg actcactata gg                                                22


<210>  42
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #1 Sequences on the microarray (5' to 3')

<400>  42
ccactgttgc aaagttatac tcttgcaggt catcggcctt tttttttt                    48


<210>  43
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #2 Sequences on the microarray (5' to 3')

<400>  43
actcttgcag gtcacggccc actgttgcaa agttatcctt tttttttt                    48


<210>  44
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #3 Sequences on the microarray (5' to 3')

<400>  44
ttggtcaggc cgtgcttgga ctgggccatg gtggcgacct atagtgagtc gtattactgt       60

ttttttttt                                                               69


<210>  45
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #4 Sequences on the microarray (5' to 3')

<400>  45
acgcagccct ccatgcggtc ttcatggtca tctcctatag tgagtcgtat tactgttttt       60

ttttt                                                                   65


<210>  46
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #5 Sequences on the microarray (5' to 3')

<400>  46
tcgccggtga tcacgaactt gtggccgtcc tatagtgagt cgtattactg tttttttttt       60


<210>  47
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #6 Sequences on the microarray (5' to 3')

<400>  47
tgcttgccct tgaaggggta gccgatgccc tatagtgagt cgtattactg tttttttttt       60


<210>  48
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #7 Sequences on the microarray (5' to 3')

<400>  48
tcggcgaagg gcaaggggcc gccctccacc acgcacaggt tgatggccta tagtgagtcg       60

tattactgtt tttttttt                                                     78


<210>  49
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #8 Sequences on the microarray (5' to 3')

<400>  49
tgggggtact cggtgaacac gcggttgccg tacatgaagg cggcggacaa gatgtcctat       60

agtgagtcgt attactgttt ttttttt                                           87


<210>  50
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #9 Sequences on the microarray (5' to 3')

<400>  50
ggcggggcag gagttcttga agtagtcgac gatgtcctat agtgagtcgt attactgttt       60

ttttttt                                                                 67


<210>  51
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #10 Sequences on the microarray (5' to 3')

<400>  51
tcgaacagga aggagcggtc ccaggtgtag cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  52
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #11 Sequences on the miccorarray (5' to 3')

<400>  52
acgctcacgg tgatgtcggc gttgcagatg cacacggcgc cgtcctatag tgagtcgtat       60

tactgttttt ttttt                                                        75


<210>  53
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #12 Sequences on the miccorarray (5' to 3')

<400>  53
gtagaacttg gactcgtggt acatgcagtt ctcctcctat agtgagtcgt attactgttt       60

ttttttt                                                                 67


<210>  54
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #13 Sequences on the microarray (5' to 3')

<400>  54
agttgtcggt catcttcttc atcacggggc cgtcggcggg gaagttcacg cctatagtga       60

gtcgtattac tgtttttttt tt                                                82


<210>  55
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #14 Sequences on the microarray (5' to 3')

<400>  55
tgcttgggca cggggatgat cttctcgcag gagggctccc tatagtgagt cgtattactg       60

tttttttttt                                                              70


<210>  56
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #15 Sequences on the microarray (5' to 3')

<400>  56
ttcagcagca ggtacatgct cacgtcgccc ttcaagatgc cctatagtga gtcgtattac       60

tgtttttttt tt                                                           72


<210>  57
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #16 Sequences on the microarray (5' to 3')

<400>  57
ttgtacacgg tgtcgaactg gcagcgcaag cggccaccgt cctatagtga gtcgtattac       60

tgtttttttt tt                                                           72


<210>  58
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #17 Sequences on the microarray (5' to 3')

<400>  58
agtcgggcat cttgcggggc acggacttgg cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  59
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #18 Sequences on the microarray (5' to 3')

<400>  59
tcgcgggtca gcttgtgctg gatgaagtgc ctatagtgag tcgtattact gttttttttt       60

t                                                                       61


<210>  60
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #19 Sequences on the microarray (5' to 3')

<400>  60
ggaggcgatg gcgtgctcgg tcaggtgcca cttctggttc ttggcgtcgc tgcggtccta       60

tagtgagtcg tattactgtt tttttttt                                          88


<210>  61
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #20 Sequences on the microarray (5' to 3')

<400>  61
tcagggcaag gcggagccta tagtgagtcg tattactgtt tttttttt                    48


<210>  62
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #21 Sequences on the microarray (5' to 3')

<400>  62
gcacggcctg accaaggaga tgaccatgaa cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  63
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #22 Sequences on the microarray (5' to 3')

<400>  63
catggagggc tgcgtggacg gccacaagtt cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  64
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #23 Sequences on the microarray (5' to 3')

<400>  64
cgtgatcacc ggcgagggca tcggctaccc tatagtgagt cgtattactg tttttttttt       60


<210>  65
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #24  Sequences on the microarray (5' to 3')

<400>  65
cttcaagggc aagcaggcca tcaacctgtg cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  66
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #25 Sequences on the microarray (5' to 3')

<400>  66
cttgcccttc gccgaggaca tcttgtccgc ctatagtgag tcgtattact gttttttttt       60

t                                                                       61


<210>  67
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #26 Sequences on the microarray (5' to 3')

<400>  67
caccgagtac ccccaggaca tcgtcgacta cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  68
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #27 Sequences on the microarray (5' to 3')

<400>  68
aactcctgcc ccgccggcta cacctgggac ctatagtgag tcgtattact gttttttttt       60

t                                                                       61


<210>  69
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #28 Sequences on the microarray (5' to 3')

<400>  69
ctccttcctg ttcgaggacg gcgccgtgtg cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  70
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #29 Sequences on the microarray (5' to 3')

<400>  70
catcaccgtg agcgtggagg agaactgcat cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  71
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #30 Sequences on the microarray (5' to 3')

<400>  71
gagtccaagt tctacggcgt gaacttcccc tatagtgagt cgtattactg tttttttttt       60


<210>  72
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #31 Sequences on the microarray (5' to 3')

<400>  72
agatgaccga caactgggag ccctcctgcg cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  73
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #32 Sequences on the microarray (5' to 3')

<400>  73
ccccgtgccc aagcagggca tcttgaaggg cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  74
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #33 Sequences on the microarray (5' to 3')

<400>  74
gtacctgctg ctgaaggacg gtggccgctt cctatagtga gtcgtattac tgtttttttt       60

tt                                                                      62


<210>  75
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #34 Sequences on the microarray (5' to 3')

<400>  75
cgacaccgtg tacaaggcca agtccgtgcc tatagtgagt cgtattactg tttttttttt       60


<210>  76
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #35 Sequences on the microarray (5' to 3')

<400>  76
gcaagatgcc cgactggcac ttcatccagc ctatagtgag tcgtattact gttttttttt       60

t                                                                       61


<210>  77
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #36 Sequences on the microarray (5' to 3')

<400>  77
caagctgacc cgcgaggacc gcagcgacgc ctatagtgag tcgtattact gttttttttt       60

t                                                                       61


<210>  78
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  #37 Sequences on the microarray (5' to 3')

<400>  78
cacgccatcg cctccggctc cgccttgccc tatagtgagt cgtattactg tttttttttt       60


