                         SEQUENCE LISTING

<110>  THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
       MONTEFIORE MEDICAL CENTER
       KITASATO UNIVERSITY
       GENSURE, Robert C.
       PONNAPAKKAM, Tulasi
       KATIKANENI, Ranjitha
       PHILOMINATHAN, Sagaya Theresa Leena
       KOIDE, Takaki
       MATSUSHITA, Osamu
       SAKON, Joshua
 
<120>  DELIVERY OF THERAPEUTIC AGENTS BY A COLLAGEN BINDING PROTEIN 

<130>  5965-00036

<150>  US 61/596,869
<151>  2012-02-09

<150>  WO PCT/US2012/069831
<151>  2012-12-14

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic fusion protein containing parathyroid hormone segment 
       and collagen-binding domain

<400>  1

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Gly Ile Asn Ser Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro 
        35                  40                  45              


Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro 
    50                  55                  60                  


Val Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe 
65                  70                  75                  80  


Asp Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly 
                85                  90                  95      


Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val 
            100                 105                 110         


Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala 
        115                 120                 125             


Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn Gly Ser 
    130                 135                 140                 


Tyr Met Pro Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
145                 150                 155             


<210>  2
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic fusion protein containing parathyroid hormone fragment 
       and collagen-binding domain and polycystic kidney disease domain 
       of ColH.

<400>  2

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Gly Ile Pro Glu Ile Lys Asp Leu Ser Glu Asn Lys Leu Pro Val 
        35                  40                  45              


Ile Tyr Met His Val Pro Lys Ser Gly Ala Leu Asn Gln Lys Val Val 
    50                  55                  60                  


Phe Tyr Gly Lys Gly Thr Tyr Asp Pro Asp Gly Ser Ile Ala Gly Tyr 
65                  70                  75                  80  


Gln Trp Asp Phe Gly Asp Gly Ser Asp Phe Ser Ser Glu Gln Asn Pro 
                85                  90                  95      


Ser His Val Tyr Thr Lys Lys Gly Glu Tyr Thr Val Thr Leu Arg Val 
            100                 105                 110         


Met Asp Ser Ser Gly Gln Met Ser Glu Lys Thr Met Lys Ile Lys Ile 
        115                 120                 125             


Thr Asp Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser 
    130                 135                 140                 


Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val Ser Gly 
145                 150                 155                 160 


Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile 
                165                 170                 175     


Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly 
            180                 185                 190         


Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala 
        195                 200                 205             


Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro 
    210                 215                 220                 


Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro 
225                 230                 235                 240 


Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
                245                 250     


<210>  3
<211>  5464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic expression vector

<400>  3
agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg       60

gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      120

tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc      180

tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca      240

cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc      300

aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc      360

gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc      420

ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata      480

tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc      540

ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact      600

ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag      660

atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt      720

tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa      780

aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat      840

ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc      900

atcctccaaa atcggatctg atcgaaggtc gttctgtgag tgaaatacag cttatgcata      960

acctgggaaa acatctgaac tcgatggaga gagtagaatg gctgcgtaag aagctgcagg     1020

atgtgcacaa tggaattaat tccccggtat atccaatagg cactgaaaaa gaaccaaata     1080

acagtaaaga aactgcaagt ggtccaatag taccaggtat acctgttagt ggaaccatag     1140

aaaatacaag tgatcaagat tatttctatt ttgatgttat aacaccagga gaagtaaaaa     1200

tagatataaa taaattaggg tacggaggag ctacttgggt agtatatgat gaaaataata     1260

atgcagtatc ttatgccact gatgatgggc aaaatttaag tggaaagttt aaggcagata     1320

aaccaggtag atattacatc catctttaca tgtttaatgg tagttatatg ccatatagaa     1380

ttaatataga aggttcagta ggaagataat attttattag ttgaggtaac tccactcgaa     1440

ttggtcgact cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg     1500

atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag     1560

cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg     1620

gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg     1680

gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt     1740

caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac     1800

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     1860

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat     1920

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc     1980

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga     2040

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg     2100

cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc     2160

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag     2220

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc     2280

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg     2340

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg     2400

acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac     2460

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac     2520

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg     2580

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg     2640

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg     2700

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     2760

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     2820

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     2880

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     2940

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     3000

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     3060

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     3120

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     3180

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     3240

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     3300

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     3360

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     3420

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     3480

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     3540

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     3600

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     3660

aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac     3720

accgcataaa ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc     3780

gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg     3840

cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg     3900

tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca     3960

accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca     4020

gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac     4080

tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg     4140

cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg     4200

accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg     4260

tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg     4320

gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa     4380

gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa     4440

ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca     4500

tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg     4560

cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg     4620

tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca     4680

aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg     4740

gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc     4800

tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg     4860

cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag     4920

ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga     4980

attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cggattcact     5040

ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct     5100

tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc     5160

ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga     5220

agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc     5280

ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat     5340

tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt     5400

taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg     5460

aatt                                                                  5464


<210>  4
<211>  383
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic GST-PTH-CBD fusion protein

<400>  4

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      


Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          


Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              


Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  


Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  


Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      


Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         


Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             


Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 


Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 


Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     


Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         


Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             


Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Ile Glu Gly 
    210                 215                 220                 


Arg Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu 
225                 230                 235                 240 


Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val 
                245                 250                 255     


His Asn Gly Ile Asn Ser Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu 
            260                 265                 270         


Pro Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile 
        275                 280                 285             


Pro Val Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr 
    290                 295                 300                 


Phe Asp Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu 
305                 310                 315                 320 


Gly Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala 
                325                 330                 335     


Val Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys 
            340                 345                 350         


Ala Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn Gly 
        355                 360                 365             


Ser Tyr Met Pro Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
    370                 375                 380             


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide: Factor Xa recognition sequence

<400>  5

Ile Glu Gly Arg 
1               


<210>  6
<211>  1021
<212>  PRT
<213>  Clostridium histolyticum

<400>  6

Met Lys Arg Lys Cys Leu Ser Lys Arg Leu Met Leu Ala Ile Thr Met 
1               5                   10                  15      


Ala Thr Ile Phe Thr Val Asn Ser Thr Leu Pro Ile Tyr Ala Ala Val 
            20                  25                  30          


Asp Lys Asn Asn Ala Thr Ala Ala Val Gln Asn Glu Ser Lys Arg Tyr 
        35                  40                  45              


Thr Val Ser Tyr Leu Lys Thr Leu Asn Tyr Tyr Asp Leu Val Asp Leu 
    50                  55                  60                  


Leu Val Lys Thr Glu Ile Glu Asn Leu Pro Asp Leu Phe Gln Tyr Ser 
65                  70                  75                  80  


Ser Asp Ala Lys Glu Phe Tyr Gly Asn Lys Thr Arg Met Ser Phe Ile 
                85                  90                  95      


Met Asp Glu Ile Gly Arg Arg Ala Pro Gln Tyr Thr Glu Ile Asp His 
            100                 105                 110         


Lys Gly Ile Pro Thr Leu Val Glu Val Val Arg Ala Gly Phe Tyr Leu 
        115                 120                 125             


Gly Phe His Asn Lys Glu Leu Asn Glu Ile Asn Lys Arg Ser Phe Lys 
    130                 135                 140                 


Glu Arg Val Ile Pro Ser Ile Leu Ala Ile Gln Lys Asn Pro Asn Phe 
145                 150                 155                 160 


Lys Leu Gly Thr Glu Val Gln Asp Lys Ile Val Ser Ala Thr Gly Leu 
                165                 170                 175     


Leu Ala Gly Asn Glu Thr Ala Pro Pro Glu Val Val Asn Asn Phe Thr 
            180                 185                 190         


Pro Ile Leu Gln Asp Cys Ile Lys Asn Ile Asp Arg Tyr Ala Leu Asp 
        195                 200                 205             


Asp Leu Lys Ser Lys Ala Leu Phe Asn Val Leu Ala Ala Pro Thr Tyr 
    210                 215                 220                 


Asp Ile Thr Glu Tyr Leu Arg Ala Thr Lys Glu Lys Pro Glu Asn Thr 
225                 230                 235                 240 


Pro Trp Tyr Gly Lys Ile Asp Gly Phe Ile Asn Glu Leu Lys Lys Leu 
                245                 250                 255     


Ala Leu Tyr Gly Lys Ile Asn Asp Asn Asn Ser Trp Ile Ile Asp Asn 
            260                 265                 270         


Gly Ile Tyr His Ile Ala Pro Leu Gly Lys Leu His Ser Asn Asn Lys 
        275                 280                 285             


Ile Gly Ile Glu Thr Leu Thr Glu Val Met Lys Val Tyr Pro Tyr Leu 
    290                 295                 300                 


Ser Met Gln His Leu Gln Ser Ala Asp Gln Ile Lys Arg His Tyr Asp 
305                 310                 315                 320 


Ser Lys Asp Ala Glu Gly Asn Lys Ile Pro Leu Asp Lys Phe Lys Lys 
                325                 330                 335     


Glu Gly Lys Glu Lys Tyr Cys Pro Lys Thr Tyr Thr Phe Asp Asp Gly 
            340                 345                 350         


Lys Val Ile Ile Lys Ala Gly Ala Arg Val Glu Glu Glu Lys Val Lys 
        355                 360                 365             


Arg Leu Tyr Trp Ala Ser Lys Glu Val Asn Ser Gln Phe Phe Arg Val 
    370                 375                 380                 


Tyr Gly Ile Asp Lys Pro Leu Glu Glu Gly Asn Pro Asp Asp Ile Leu 
385                 390                 395                 400 


Thr Met Val Ile Tyr Asn Ser Pro Glu Glu Tyr Lys Leu Asn Ser Val 
                405                 410                 415     


Leu Tyr Gly Tyr Asp Thr Asn Asn Gly Gly Met Tyr Ile Glu Pro Glu 
            420                 425                 430         


Gly Thr Phe Phe Thr Tyr Glu Arg Glu Ala Gln Glu Ser Thr Tyr Thr 
        435                 440                 445             


Leu Glu Glu Leu Phe Arg His Glu Tyr Thr His Tyr Leu Gln Gly Arg 
    450                 455                 460                 


Tyr Ala Val Pro Gly Gln Trp Gly Arg Thr Lys Leu Tyr Asp Asn Asp 
465                 470                 475                 480 


Arg Leu Thr Trp Tyr Glu Glu Gly Gly Ala Glu Leu Phe Ala Gly Ser 
                485                 490                 495     


Thr Arg Thr Ser Gly Ile Leu Pro Arg Lys Ser Ile Val Ser Asn Ile 
            500                 505                 510         


His Asn Thr Thr Arg Asn Asn Arg Tyr Lys Leu Ser Asp Thr Val His 
        515                 520                 525             


Ser Lys Tyr Gly Ala Ser Phe Glu Phe Tyr Asn Tyr Ala Cys Met Phe 
    530                 535                 540                 


Met Asp Tyr Met Tyr Asn Lys Asp Met Gly Ile Leu Asn Lys Leu Asn 
545                 550                 555                 560 


Asp Leu Ala Lys Asn Asn Asp Val Asp Gly Tyr Asp Asn Tyr Ile Arg 
                565                 570                 575     


Asp Leu Ser Ser Asn Tyr Ala Leu Asn Asp Lys Tyr Gln Asp His Met 
            580                 585                 590         


Gln Glu Arg Ile Asp Asn Tyr Glu Asn Leu Thr Val Pro Phe Val Ala 
        595                 600                 605             


Asp Asp Tyr Leu Val Arg His Ala Tyr Lys Asn Pro Asn Glu Ile Tyr 
    610                 615                 620                 


Ser Glu Ile Ser Glu Val Ala Lys Leu Lys Asp Ala Lys Ser Glu Val 
625                 630                 635                 640 


Lys Lys Ser Gln Tyr Phe Ser Thr Phe Thr Leu Arg Gly Ser Tyr Thr 
                645                 650                 655     


Gly Gly Ala Ser Lys Gly Lys Leu Glu Asp Gln Lys Ala Met Asn Lys 
            660                 665                 670         


Phe Ile Asp Asp Ser Leu Lys Lys Leu Asp Thr Tyr Ser Trp Ser Gly 
        675                 680                 685             


Tyr Lys Thr Leu Thr Ala Tyr Phe Thr Asn Tyr Lys Val Asp Ser Ser 
    690                 695                 700                 


Asn Arg Val Thr Tyr Asp Val Val Phe His Gly Tyr Leu Pro Asn Glu 
705                 710                 715                 720 


Gly Asp Ser Lys Asn Ser Leu Pro Tyr Gly Lys Ile Asn Gly Thr Tyr 
                725                 730                 735     


Lys Gly Thr Glu Lys Glu Lys Ile Lys Phe Ser Ser Glu Gly Ser Phe 
            740                 745                 750         


Asp Pro Asp Gly Lys Ile Val Ser Tyr Glu Trp Asp Phe Gly Asp Gly 
        755                 760                 765             


Asn Lys Ser Asn Glu Glu Asn Pro Glu His Ser Tyr Asp Lys Val Gly 
    770                 775                 780                 


Thr Tyr Thr Val Lys Leu Lys Val Thr Asp Asp Lys Gly Glu Ser Ser 
785                 790                 795                 800 


Val Ser Thr Thr Thr Ala Glu Ile Lys Asp Leu Ser Glu Asn Lys Leu 
                805                 810                 815     


Pro Val Ile Tyr Met His Val Pro Lys Ser Gly Ala Leu Asn Gln Lys 
            820                 825                 830         


Val Val Phe Tyr Gly Lys Gly Thr Tyr Asp Pro Asp Gly Ser Ile Ala 
        835                 840                 845             


Gly Tyr Gln Trp Asp Phe Gly Asp Gly Ser Asp Phe Ser Ser Glu Gln 
    850                 855                 860                 


Asn Pro Ser His Val Tyr Thr Lys Lys Gly Glu Tyr Thr Val Thr Leu 
865                 870                 875                 880 


Arg Val Met Asp Ser Ser Gly Gln Met Ser Glu Lys Thr Met Lys Ile 
                885                 890                 895     


Lys Ile Thr Asp Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn 
            900                 905                 910         


Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val 
        915                 920                 925             


Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp 
    930                 935                 940                 


Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr 
945                 950                 955                 960 


Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser 
                965                 970                 975     


Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp 
            980                 985                 990         


Lys Pro Gly Arg Tyr Tyr Ile His  Leu Tyr Met Phe Asn  Gly Ser Tyr 
        995                 1000                 1005             


Met Pro  Tyr Arg Ile Asn Ile  Glu Gly Ser Val Gly  Arg 
    1010                 1015                 1020     


<210>  7
<211>  84
<212>  PRT
<213>  Homo sapiens

<400>  7

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Phe Val Ala Leu Gly Ala Pro Leu Ala Pro Arg Asp Ala Gly Ser 
        35                  40                  45              


Gln Arg Pro Arg Lys Lys Glu Asp Asn Val Leu Val Glu Ser His Glu 
    50                  55                  60                  


Lys Ser Leu Gly Glu Ala Asp Lys Ala Asp Val Asn Val Leu Thr Lys 
65                  70                  75                  80  


Ala Lys Ser Gln 
                


<210>  8
<211>  141
<212>  PRT
<213>  Homo sapiens

<400>  8

Ala Val Ser Glu His Gln Leu Leu His Asp Lys Gly Lys Ser Ile Gln 
1               5                   10                  15      


Asp Leu Arg Arg Arg Phe Phe Leu His His Leu Ile Ala Glu Ile His 
            20                  25                  30          


Thr Ala Glu Ile Arg Ala Thr Ser Glu Val Ser Pro Asn Ser Lys Pro 
        35                  40                  45              


Ser Pro Asn Thr Lys Asn His Pro Val Arg Phe Gly Ser Asp Asp Glu 
    50                  55                  60                  


Gly Arg Tyr Leu Thr Gln Glu Thr Asn Lys Val Glu Thr Tyr Lys Glu 
65                  70                  75                  80  


Gln Pro Leu Lys Thr Pro Gly Lys Lys Lys Lys Gly Lys Pro Gly Lys 
                85                  90                  95      


Arg Lys Glu Gln Glu Lys Lys Lys Arg Arg Thr Arg Ser Ala Trp Leu 
            100                 105                 110         


Asp Ser Gly Val Thr Gly Ser Gly Leu Glu Gly Asp His Leu Ser Asp 
        115                 120                 125             


Thr Ser Thr Thr Ser Leu Glu Leu Asp Ser Arg Arg His 
    130                 135                 140     


<210>  9
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Gly-Ser-PTH(1-33)-CBD fusion protein

<400>  9

Gly Ser Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His 
1               5                   10                  15      


Leu Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp 
            20                  25                  30          


Val His Asn Gly Ile Asn Ser Pro Val Tyr Pro Ile Gly Thr Glu Lys 
        35                  40                  45              


Glu Pro Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly 
    50                  55                  60                  


Ile Pro Val Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe 
65                  70                  75                  80  


Tyr Phe Asp Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys 
                85                  90                  95      


Leu Gly Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn 
            100                 105                 110         


Ala Val Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe 
        115                 120                 125             


Lys Ala Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu Tyr Met Phe Asn 
    130                 135                 140                 


Gly Ser Tyr Met Pro Tyr Arg Ile Asn Ile Glu Gly Ser Val Gly Arg 
145                 150                 155                 160 


<210>  10
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PTH(7-33)-CBD fusion protein

<400>  10

Leu Met His Asn Leu Gly Lys His Leu Asn Ser Met Glu Arg Val Glu 
1               5                   10                  15      


Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn Gly Ile Asn Ser Pro 
            20                  25                  30          


Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser Lys Glu Thr 
        35                  40                  45              


Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val Ser Gly Thr Ile Glu 
    50                  55                  60                  


Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile Thr Pro Gly 
65                  70                  75                  80  


Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly Ala Thr Trp 
                85                  90                  95      


Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala Thr Asp Asp 
            100                 105                 110         


Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro Gly Arg Tyr 
        115                 120                 125             


Tyr Ile His Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro Tyr Arg Ile 
    130                 135                 140                 


Asn Ile Glu Gly Ser Val Gly Arg 
145                 150         


<210>  11
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PTH(1-33) with Gly-Ser amino terminal extension

<400>  11

Gly Ser Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His 
1               5                   10                  15      


Leu Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp 
            20                  25                  30          


Val His Asn 
        35  


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic thrombin cleavage sequence

<400>  12

Leu Val Pro Arg Gly Ser 
1               5       


<210>  13
<211>  111
<212>  PRT
<213>  Clostridium histolyticum

<400>  13

Leu Lys Glu Lys Glu Asn Asn Asp Ser Ser Asp Lys Ala Thr Val Ile 
1               5                   10                  15      


Pro Asn Phe Asn Thr Thr Met Gln Gly Ser Leu Leu Gly Asp Asp Ser 
            20                  25                  30          


Arg Asp Tyr Tyr Ser Phe Glu Val Lys Glu Glu Gly Glu Val Asn Ile 
        35                  40                  45              


Glu Leu Asp Lys Lys Asp Glu Phe Gly Val Thr Trp Thr Leu His Pro 
    50                  55                  60                  


Glu Ser Asn Ile Asn Asp Arg Ile Thr Tyr Gly Gln Val Asp Gly Asn 
65                  70                  75                  80  


Lys Val Ser Asn Lys Val Lys Leu Arg Pro Gly Lys Tyr Tyr Leu Leu 
                85                  90                  95      


Val Tyr Lys Tyr Ser Gly Ser Gly Asn Tyr Glu Leu Arg Val Asn 
            100                 105                 110     


<210>  14
<211>  111
<212>  PRT
<213>  C. sporogenes

<400>  14

Ile His Glu Lys Glu Asn Asn Asp Ser Phe Glu Ser Ala Asn Lys Ile 
1               5                   10                  15      


Val Leu Asn Ala Pro Ile Leu Gly Ser Leu Asn Gly Glu Asp Leu Arg 
            20                  25                  30          


Asp Ile Tyr Ser Phe Glu Ile Lys Glu Thr Lys Asp Leu Asn Ile Lys 
        35                  40                  45              


Leu Thr Asn Leu Asn Asn Leu Gly Leu Thr Trp Thr Leu Tyr Lys Glu 
    50                  55                  60                  


Ser Asp Leu Asn Asn Tyr Ile Ala Tyr Gly Ser Lys Leu Gly Ser Thr 
65                  70                  75                  80  


Ile Val Gly Asn Cys His Val Thr Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Tyr Ser Gly Asn Asn Gly Asn Tyr Ser Leu Ile Ile Lys 
            100                 105                 110     


<210>  15
<211>  111
<212>  PRT
<213>  C. botulinum

<400>  15

Ile Tyr Glu Lys Glu Asn Asn Asp Ser Phe Glu Thr Ala Asn Lys Ile 
1               5                   10                  15      


Met Leu Asn Thr Thr Val Leu Gly Asn Leu Asn Gly Lys Asp Val Arg 
            20                  25                  30          


Asp Ile Tyr Ser Phe Asp Ile Lys Glu Ala Lys Asp Leu Asp Ile Lys 
        35                  40                  45              


Leu Asn Asn Leu Asn Asn Leu Gly Leu Ala Trp Asn Leu Tyr Lys Glu 
    50                  55                  60                  


Ser Asp Leu Asn Asn Tyr Ile Ala Tyr Gly Ser Val Ser Gly Asn Thr 
65                  70                  75                  80  


Ile Lys Gly Lys Cys Asn Val Ala Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Tyr Ser Gly Asp Asn Gly Asn Tyr Ser Leu Ala Ile Lys 
            100                 105                 110     


<210>  16
<211>  111
<212>  PRT
<213>  B. cereus

<400>  16

Leu Thr Glu Ser Glu Pro Asn Asn Arg Pro Glu Glu Ala Asn Arg Ile 
1               5                   10                  15      


Gly Leu Asn Thr Thr Ile Lys Gly Ser Leu Ile Gly Gly Asp His Thr 
            20                  25                  30          


Asp Val Tyr Thr Phe Asn Val Ala Ser Ala Lys Asn Ile Asn Ile Ser 
        35                  40                  45              


Val Leu Asn Glu Tyr Gly Ile Gly Met Thr Trp Val Leu His His Glu 
    50                  55                  60                  


Ser Asp Met Gln Asn Tyr Ala Ala Tyr Gly Gln Val Asn Gly Asn His 
65                  70                  75                  80  


Ile Glu Ala Asn Phe Asn Ala Lys Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Tyr Asp Asn Gly Asp Gly Thr Tyr Glu Leu Ser Val Lys 
            100                 105                 110     


<210>  17
<211>  111
<212>  PRT
<213>  B. anthracis

<400>  17

Leu Thr Glu Ser Glu Pro Asn Asn Arg Pro Glu Glu Ala Asn Arg Ile 
1               5                   10                  15      


Gly Leu Asn Thr Thr Ile Lys Gly Ser Leu Ile Gly Gly Asp His Thr 
            20                  25                  30          


Asp Val Tyr Thr Phe Asn Val Ala Ser Ala Lys Asn Ile Asp Ile Ser 
        35                  40                  45              


Val Leu Asn Glu Tyr Gly Ile Gly Met Thr Trp Val Leu His His Glu 
    50                  55                  60                  


Ser Asp Met Gln Asn Tyr Ala Ala Tyr Gly Gln Ala Asn Gly Asn His 
65                  70                  75                  80  


Ile Glu Ala Asn Phe Asn Ala Lys Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Tyr Asp Asn Gly Asp Gly Thr Tyr Glu Leu Ser Val Lys 
            100                 105                 110     


<210>  18
<211>  111
<212>  PRT
<213>  Bacillus sp.

<400>  18

Lys Thr Glu Ile Glu Pro Asn Asn Arg Pro Glu Glu Ala Thr Met Leu 
1               5                   10                  15      


Pro Phe Asn Thr Pro Leu Ser Gly Ser Leu Met Glu Asp Asp His Thr 
            20                  25                  30          


Asp Val Tyr Glu Phe Asn Val Thr Ser Pro Lys Glu Ile Asp Ile Ser 
        35                  40                  45              


Val Leu Asn Glu Asn Gln Ile Gly Met Thr Trp Val Leu Tyr His Glu 
    50                  55                  60                  


Ser Asp Ser Gln Asn Tyr Ala Ser Phe Gly Gln Glu Asp Gly Asn Met 
65                  70                  75                  80  


Ile Asn Gly Lys Trp Asn Ala Lys Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Phe Glu Asn Glu Asn Gly Thr Tyr Thr Val His Val Gln 
            100                 105                 110     


<210>  19
<211>  111
<212>  PRT
<213>  L. sphaericus

<400>  19

Lys Ala Glu Ile Glu Pro Asn Asn Arg Pro Glu Glu Ala Thr Ile Leu 
1               5                   10                  15      


Pro Phe Asn Thr Pro Leu Lys Gly Arg Leu Met Asp Asp Asp His Thr 
            20                  25                  30          


Asp Val Tyr Glu Phe Asn Val Thr Ser Pro Lys Glu Leu Asp Ile Ser 
        35                  40                  45              


Val Leu Asn Glu Asn Arg Ile Gly Met Thr Trp Val Leu Tyr His Glu 
    50                  55                  60                  


Ser Asp Ser Gln Asn Tyr Ala Ser Phe Gly Gln Glu Glu Gly Asn Met 
65                  70                  75                  80  


Ile Asn Gly Lys Leu His Ala Glu Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Phe Glu Asn Glu Asn Gly Thr Tyr Thr Val Gln Val Gln 
            100                 105                 110     


<210>  20
<211>  111
<212>  PRT
<213>  C. cerus

<400>  20

Val Thr Glu Asn Glu Pro Asn Asn Glu Pro Arg Gln Ala Asn Lys Val 
1               5                   10                  15      


Asn Phe His Thr Pro Val Lys Gly Thr Leu His Asn Ser Asp Arg Val 
            20                  25                  30          


Asp Val Phe Thr Phe Gln Ile Asp Ser Pro Glu Asn Ile Asn Ile Ser 
        35                  40                  45              


Leu Leu Asn Glu Gln Asn Ile Gly Met Thr Trp Val Leu His His Glu 
    50                  55                  60                  


Ser Asp Leu Asn Asn Tyr Val Ala Tyr Gly Glu Asn Glu Gly Asn Val 
65                  70                  75                  80  


Val Lys Gly Thr Tyr Asn Ala Lys Pro Gly Lys Tyr Tyr Leu Tyr Val 
                85                  90                  95      


Tyr Lys Tyr Glu Asn Lys Asp Gly Ser Tyr Val Leu Asn Ile Lys 
            100                 105                 110     


<210>  21
<211>  111
<212>  PRT
<213>  B. mycoides

<400>  21

Ser Val Glu Lys Glu Pro Asn Asn Ser Phe Gln Thr Ala Asn Lys Leu 
1               5                   10                  15      


Gln Leu Asn Gln Leu Leu Arg Ala Ser Leu Gly Asn Gly Asp Thr Ser 
            20                  25                  30          


Asp Tyr Phe Glu Ile Asn Val Glu Thr Ala Arg Asn Leu Gln Ile Asn 
        35                  40                  45              


Val Thr Lys Glu Asn Asn Ile Gly Val Asn Trp Val Leu Tyr Ser Ala 
    50                  55                  60                  


Ala Asp Leu Asn Asn Tyr Val Thr Tyr Ala Gln Thr Gln Gly Asn Lys 
65                  70                  75                  80  


Leu Val Gly Ser Tyr Asn Ala His Pro Gly Lys Tyr Tyr Leu His Val 
                85                  90                  95      


Tyr Gln Tyr Gly Gly Gly Thr Gly Asn Tyr Thr Val Glu Val Lys 
            100                 105                 110     


<210>  22
<211>  112
<212>  PRT
<213>  B. weihensteph

<400>  22

Ala Val Glu Lys Glu Pro Asn Asn Ser Phe Asp Ala Ala Asn Pro Leu 
1               5                   10                  15      


Ser Leu Asn Ala Leu Leu Arg Gly Asn Leu Ser Asp Gln Asp Gln Val 
            20                  25                  30          


Asp Arg Phe Val Ile Asp Val Lys Asp Pro Lys Asp Leu Gln Ile Thr 
        35                  40                  45              


Val Thr Asn Glu Gln Asn Leu Gly Leu Asn Trp Val Leu Tyr Ser Glu 
    50                  55                  60                  


Ser Asp Leu Asn Asn Tyr Val Thr Tyr Ala Thr Lys Arg Asp Gly Asn 
65                  70                  75                  80  


Lys Leu Leu Gly Asn Tyr Asn Ala Lys Pro Gly Lys Tyr Tyr Leu Ser 
                85                  90                  95      


Val Tyr Lys Tyr Gly Gly Gly Thr Gly Asn Phe Thr Val Glu Val Lys 
            100                 105                 110         


<210>  23
<211>  111
<212>  PRT
<213>  B. brevis

<400>  23

Glu Lys Glu Gln Glu Pro Asn Asn Ser Phe Ser Glu Ala Asn Pro Leu 
1               5                   10                  15      


Lys Ser Asn Val Glu Leu Ser Gly Gln Thr Ser Lys Gln Asp Asp Lys 
            20                  25                  30          


Asp Ile Phe Ala Leu Lys Val Leu Gly Asn Gly Thr Val Lys Ile Asn 
        35                  40                  45              


Val Thr Ser Glu His Asp Thr Gly Leu Asn Trp Val Val His His Glu 
    50                  55                  60                  


Asp Asp Leu Asn Asn Tyr Leu Ala Tyr Pro Lys Thr Ser Gly Lys Thr 
65                  70                  75                  80  


Leu Ser Gly Glu Phe Glu Ala Thr Pro Gly Thr Tyr Tyr Leu Ser Val 
                85                  90                  95      


Tyr Asn Phe Asn Gly Glu Thr Ile Pro Tyr Lys Val Thr Ala Glu 
            100                 105                 110     


<210>  24
<211>  111
<212>  PRT
<213>  B. brevis

<400>  24

Pro Thr Glu Val Glu Pro Asn Asn Ser Phe Asp Asp Ala Asn Thr Leu 
1               5                   10                  15      


Gln Leu Gly Lys Glu Ile Ser Gly Gln Thr Asp Arg Thr Asp Asp Lys 
            20                  25                  30          


Asp Thr Tyr Met Ile Gln Val Glu Glu Glu Gly Val Ile Gln Val Thr 
        35                  40                  45              


Val Ser Ser Glu Lys Asp Glu Gly Leu Asn Trp Val Val Phe His Glu 
    50                  55                  60                  


Asp Asp Leu Lys Thr Tyr Phe Ala Tyr Pro Lys Thr Thr Gly Lys Lys 
65                  70                  75                  80  


Leu Thr Gly Glu Phe Glu Ala Lys Pro Gly Lys Tyr Tyr Leu Leu Val 
                85                  90                  95      


Tyr Asn Thr Asn Asn Thr Lys Ile Pro Tyr Lys Ala Ile Val Asn 
            100                 105                 110     


<210>  25
<211>  112
<212>  PRT
<213>  C. perfringens

<400>  25

Ile Lys Glu Val Glu Asn Asn Asn Asp Phe Asp Lys Ala Met Lys Val 
1               5                   10                  15      


Asp Ser Asn Ser Lys Ile Val Gly Thr Leu Ser Asn Asp Asp Leu Lys 
            20                  25                  30          


Asp Ile Tyr Ser Ile Asp Ile Lys Asn Pro Ser Asp Leu Asn Ile Val 
        35                  40                  45              


Val Glu Asn Leu Asp Asn Ile Lys Met Asn Trp Leu Leu Tyr Ser Ala 
    50                  55                  60                  


Asp Asp Leu Ser Asn Tyr Val Asp Tyr Ala Asn Ala Asp Gly Asn Lys 
65                  70                  75                  80  


Leu Ser Asn Thr Cys Lys Leu Asn Pro Gly Lys Tyr Tyr Leu Cys Val 
                85                  90                  95      


Tyr Gln Phe Glu Asn Ser Gly Thr Gly Asn Tyr Thr Val Asn Leu Gln 
            100                 105                 110         


<210>  26
<211>  115
<212>  PRT
<213>  C. sporogenes

<400>  26

Ile Ser Glu Lys Glu Asp Asn Asp Ser Phe Asp Lys Ala Asn Arg Val 
1               5                   10                  15      


Gly Lys Asn Gln Thr Val Leu Ala Thr Leu Asp Thr Lys Asp Asn Arg 
            20                  25                  30          


Asp Thr Tyr Tyr Phe Asp Ala Leu Ala Ala Arg Thr Ile Asp Ile Val 
        35                  40                  45              


Met Glu Asn Thr Asp Asn Asn Ser Thr Ile Phe Asn Trp Leu Ala Tyr 
    50                  55                  60                  


Ser Ser Asp Asn Thr Asn Asn Tyr Ile Gly Tyr Pro Thr Lys Lys Glu 
65                  70                  75                  80  


Gly Asn Lys Leu Met Gly Ser Phe Lys Val Pro Lys Pro Gly Arg Tyr 
                85                  90                  95      


Tyr Ile Leu Ala Tyr Lys Asn Ser Ser Asn Lys Ile Asn Tyr Lys Leu 
            100                 105                 110         


Thr Ile Asn 
        115 


<210>  27
<211>  115
<212>  PRT
<213>  C. botulinum

<400>  27

Ile Ser Glu Lys Glu Asp Asn Asn Ser Phe Asp Lys Ala Asn Arg Val 
1               5                   10                  15      


Cys Lys Asn Gln Ser Val Ile Ala Thr Leu Asp Thr Asn Asp Pro Arg 
            20                  25                  30          


Asp Thr Tyr Tyr Phe Asp Ala Leu Thr Ala Gly Asn Ile Glu Val Thr 
        35                  40                  45              


Met Gly Asn Thr Asp Asn Ser Ser Asn Glu Phe Asn Trp Leu Ala Tyr 
    50                  55                  60                  


Ser Ser Asp Asn Thr Asn Asn Tyr Ile Gly Tyr Ala Thr Lys Arg Glu 
65                  70                  75                  80  


Gly Asn Lys Ile Thr Gly Asn Phe Lys Val Asp Lys Pro Gly Arg Tyr 
                85                  90                  95      


Tyr Ile Val Ala Tyr Lys Thr Ser Ser Asn Lys Ile Asn Tyr Lys Leu 
            100                 105                 110         


Asn Ile Lys 
        115 


<210>  28
<211>  115
<212>  PRT
<213>  C. sporogenes

<400>  28

Val Ser Glu Lys Glu Asp Asn Asn Asp Phe Thr Thr Ala Asn Pro Val 
1               5                   10                  15      


Tyr Tyr Lys Asp Leu Val Asn Gly Ser Val Ser Ser Ser Asp Asn Lys 
            20                  25                  30          


Asp Thr Phe Tyr Phe Thr Val Thr Lys Pro Ser Asp Ile Thr Ile Thr 
        35                  40                  45              


Val Glu Lys Thr Asn Asn Asp Lys Ser Glu Phe Asn Trp Leu Leu Phe 
    50                  55                  60                  


Ser Asp Glu Asp Lys Ser Asn Tyr Met Ala Phe Pro Asn Lys Glu Leu 
65                  70                  75                  80  


Gly Asn Gln Leu Ser Asn Thr Val Lys Ile Asn Lys Pro Gly Lys Tyr 
                85                  90                  95      


Tyr Leu Val Ile Tyr Lys Thr Leu Gly Glu Lys Val Asp Tyr Lys Phe 
            100                 105                 110         


Ser Ile Glu 
        115 


<210>  29
<211>  115
<212>  PRT
<213>  C. botulinum

<400>  29

Val Ser Glu Lys Glu Asn Asn Asn Asp Tyr Val Asn Ala Asn Pro Val 
1               5                   10                  15      


Tyr Ser Lys Asp Leu Val Asn Gly Ser Val Ser Ser Ser Asp Asp Arg 
            20                  25                  30          


Asp Ile Phe Tyr Phe Asn Val Thr Lys Pro Ser Asp Ile Thr Ile Asn 
        35                  40                  45              


Val Glu Lys Ile Asn Lys Asp Lys Ser Glu Phe Ser Trp Leu Leu Phe 
    50                  55                  60                  


Ser Glu Glu Asp Lys Ser Asn Tyr Ile Thr Tyr Pro Asn Lys Glu Leu 
65                  70                  75                  80  


Glu Asn Leu Phe Tyr Ser Thr Val Lys Ile Asp Lys Pro Gly Lys Tyr 
                85                  90                  95      


Tyr Leu Val Ile Tyr Lys Val Ser Gly Glu Lys Ser Asp Tyr Arg Phe 
            100                 105                 110         


Asn Ile Glu 
        115 


<210>  30
<211>  113
<212>  PRT
<213>  C. sordellii

<400>  30

Gly Val Glu Gln Glu Asp Asn Asn Ser Phe Glu Lys Ala Asn Pro Phe 
1               5                   10                  15      


Ser Ile Asn Gln Leu Val Lys Gly Glu Leu Asp Asn Asn Lys Asp Thr 
            20                  25                  30          


Ser Asp Tyr Phe Lys Phe Glu Val Lys Glu Asp Ala Gln Leu Asn Ile 
        35                  40                  45              


Ser Leu Glu Lys Thr Glu Gly Asp Gly Val Asn Trp Leu Leu Phe Lys 
    50                  55                  60                  


Asp Ser Asp Leu Glu Asn Tyr Ile Ala Ser Pro Thr Glu Ser Ile Asp 
65                  70                  75                  80  


Asn Lys Leu Asn Gly Lys Val Asp Leu Lys Val Gly Thr Tyr Tyr Leu 
                85                  90                  95      


Glu Val Tyr Gly Tyr Gly Ser Ser Pro Val Lys Tyr Asn Phe Lys Val 
            100                 105                 110         


Thr 
    


<210>  31
<211>  113
<212>  PRT
<213>  Clostridium histolyticum

<400>  31

Thr Lys Glu Met Glu Pro Asn Asp Asp Ile Lys Glu Ala Asn Gly Pro 
1               5                   10                  15      


Ile Val Glu Gly Val Thr Val Lys Gly Asp Leu Asn Gly Ser Asp Asp 
            20                  25                  30          


Ala Asp Thr Phe Tyr Phe Asp Val Lys Glu Asp Gly Asp Val Thr Ile 
        35                  40                  45              


Glu Leu Pro Tyr Ser Gly Ser Ser Asn Phe Thr Trp Leu Val Tyr Lys 
    50                  55                  60                  


Glu Gly Asp Asp Gln Asn His Ile Ala Ser Gly Ile Asp Lys Asn Asn 
65                  70                  75                  80  


Ser Lys Val Gly Thr Phe Lys Ser Thr Lys Gly Arg His Tyr Val Phe 
                85                  90                  95      


Ile Tyr Lys His Asp Ser Ala Ser Asn Ile Ser Tyr Ser Leu Asn Ile 
            100                 105                 110         


Lys 
    


<210>  32
<211>  113
<212>  PRT
<213>  C. perfringens

<400>  32

Ile Asn Glu Ser Glu Pro Asn Asn Asp Phe Glu Lys Ala Asn Gln Ile 
1               5                   10                  15      


Ala Lys Ser Asn Met Leu Val Lys Gly Thr Leu Ser Glu Glu Asp Tyr 
            20                  25                  30          


Ser Asp Lys Tyr Tyr Phe Asp Val Ala Lys Lys Gly Asn Val Lys Ile 
        35                  40                  45              


Thr Leu Asn Asn Leu Asn Ser Val Gly Ile Thr Trp Thr Leu Tyr Lys 
    50                  55                  60                  


Glu Gly Asp Leu Asn Asn Tyr Val Leu Tyr Ala Thr Gly Asn Glu Gly 
65                  70                  75                  80  


Thr Val Leu Lys Gly Glu Lys Thr Leu Glu Pro Gly Arg Tyr Tyr Leu 
                85                  90                  95      


Ser Val Tyr Thr Tyr Asp Asn Gln Ser Gly Ala Tyr Thr Val Asn Val 
            100                 105                 110         


Lys 
    


<210>  33
<211>  113
<212>  PRT
<213>  C. sordellii

<400>  33

Ser Gln Glu Val Gly Asn Asp Asp Thr Phe Glu Thr Ala Asn Gly Pro 
1               5                   10                  15      


Ile Lys Ile Asn Thr Asn Tyr Ser Gly Asp Leu Ser Asp Thr Asp Asn 
            20                  25                  30          


Lys Asp Tyr Tyr Tyr Phe Asn Leu Asp Asn Pro Ser Asn Ile Asn Ile 
        35                  40                  45              


Thr Leu Glu Asn Leu Asp Asn Lys Gly Ile Ser Trp Gln Leu Phe His 
    50                  55                  60                  


Glu Ser Asp Leu Asn Asn Tyr Val Ala Tyr Pro Thr Thr Ser Gly Ala 
65                  70                  75                  80  


Ile Leu Asn Gly Asp Tyr Asn Ala Thr Lys Pro Gly Lys Tyr Tyr Ile 
                85                  90                  95      


Leu Val Tyr Asn His Asp Lys Ser Ile Ala Asn Tyr Asn Leu Lys Val 
            100                 105                 110         


Asn 
    


<210>  34
<211>  111
<212>  PRT
<213>  Clostridium histolyticum

<400>  34

Gly Thr Glu Lys Glu Pro Asn Asn Ser Lys Glu Thr Ala Ser Gly Pro 
1               5                   10                  15      


Ile Val Pro Gly Ile Pro Val Ser Gly Thr Ile Glu Asn Thr Ser Asp 
            20                  25                  30          


Gln Asp Tyr Phe Tyr Phe Asp Val Ile Thr Pro Gly Glu Val Lys Ile 
        35                  40                  45              


Asp Ile Asn Lys Leu Gly Tyr Gly Gly Ala Thr Trp Val Val Tyr Asp 
    50                  55                  60                  


Glu Asn Asn Asn Ala Val Ser Tyr Ala Thr Asp Asp Gly Gln Asn Leu 
65                  70                  75                  80  


Ser Gly Lys Phe Lys Ala Asp Lys Pro Gly Arg Tyr Tyr Ile His Leu 
                85                  90                  95      


Tyr Met Phe Asn Gly Ser Tyr Met Pro Tyr Arg Ile Asn Ile Glu 
            100                 105                 110     


<210>  35
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Any Xaa is hydroxyproline

<400>  35

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro 
1               5                   10                  15      


Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
            20                  25                  30  


<210>  36
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Any Xaa is hydroxyproline

<400>  36

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro 
1               5                   10                  15      


Xaa Gly Pro Xaa Ala Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
            20                  25                  30  


<210>  37
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Any Xaa is hydroxyproline

<400>  37

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro 
1               5                   10                  15      


Xaa Ala Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
            20                  25                  30  


<210>  38
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Any Xaa is hydroxyproline

<400>  38

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Ala Pro 
1               5                   10                  15      


Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
            20                  25                  30  


<210>  39
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Any Xaa is hydroxyproline

<400>  39

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Ala Pro Xaa Gly Pro 
1               5                   10                  15      


Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
            20                  25                  30  


<210>  40
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Any Xaa is hydroxyproline

<400>  40

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Cys Gly Pro Xaa Gly Pro 
1               5                   10                  15      


Xaa Gly Pro Xaa Gly Pro Xaa Gly 
            20                  


<210>  41
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  Any Xaa is hydroxyproline

<400>  41

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Ala Pro 
1               5                   10                  15      


Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Cys 
            20                  25                  30      


<210>  42
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  Any Xaa is hydroxyproline

<400>  42

Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
1               5                   10                  15      


Pro Xaa Gly Pro Xaa Gly 
            20          


<210>  43
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(31)
<223>  Any Xaa is hydroxyproline

<400>  43

Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
1               5                   10                  15      


Pro Xaa Gly Pro Xaa Gly Pro Arg Gly Pro Arg Gly Pro Arg Gly 
            20                  25                  30      


<210>  44
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  Any Xaa is hydroxyproline

<400>  44

Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
1               5                   


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  Any Xaa is hydroxyproline

<400>  45

Pro Xaa Gly Pro Xaa Gly Pro Xaa Ala 
1               5                   


<210>  46
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (1)..(26)
<223>  Any Xaa is hydroxyproline

<400>  46

Gly Pro Arg Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly 
1               5                   10                  15      


Pro Xaa Gly Pro Xaa Gly Pro Xaa Gly Cys 
            20                  25      


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide: GST Tag

<400>  47

Gly Ser Pro Gly Ile Pro Gly 
1               5           

 

