SEQUENCE LISTING

<110>  Evonik Industries AG
 
<120>  A cell with reduced ppGppase activity


<130>  201100331

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  2109
<212>  DNA
<213>  Escherichia coli str. K12 substr. MG1655


<220>
<221>  CDS
<222>  (1)..(2106)
<223>  spoT(b3650, ECK3640)

       Accession NP_418107; bifunctional (p)ppGpp synthetase II/ 
       guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase 
       [Escherichia coli str. K-12 substr. MG1655].

<400>  1
ttg tat ctg ttt gaa agc ctg aat caa ctg att caa acc tac ctg ccg         48
Leu Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro           
1               5                   10                  15                

gaa gac caa atc aag cgt ctg cgg cag gcg tat ctc gtt gca cgt gat         96
Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp           
            20                  25                  30                    

gct cac gag ggg caa aca cgt tca agc ggt gaa ccc tat atc acg cac        144
Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His           
        35                  40                  45                        

ccg gta gcg gtt gcc tgc att ctg gcc gag atg aaa ctc gac tat gaa        192
Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu           
    50                  55                  60                            

acg ctg atg gcg gcg ctg ctg cat gac gtg att gaa gat act ccc gcc        240
Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala           
65                  70                  75                  80            

acc tac cag gat atg gaa cag ctt ttt ggt aaa agc gtc gcc gag ctg        288
Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu           
                85                  90                  95                

gta gag ggg gtg tcg aaa ctt gat aaa ctc aag ttc cgc gat aag aaa        336
Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp Lys Lys           
            100                 105                 110                   

gag gcg cag gcc gaa aac ttt cgc aag atg att atg gcg atg gtg cag        384
Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln           
        115                 120                 125                       

gat atc cgc gtc atc ctc atc aaa ctt gcc gac cgt acc cac aac atg        432
Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met           
    130                 135                 140                           

cgc acg ctg ggc tca ctt cgc ccg gac aaa cgt cgc cgc atc gcc cgt        480
Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg           
145                 150                 155                 160           

gaa act ctc gaa att tat agc ccg ctg gcg cac cgt tta ggt atc cac        528
Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His           
                165                 170                 175               

cac att aaa acc gaa ctc gaa gag ctg ggt ttt gag gcg ctg tat ccc        576
His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro           
            180                 185                 190                   

aac cgt tat cgc gta atc aaa gaa gtg gtg aaa gcc gcg cgc ggc aac        624
Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn           
        195                 200                 205                       

cgt aaa gag atg atc cag aag att ctt tct gaa atc gaa ggg cgt ttg        672
Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu           
    210                 215                 220                           

cag gaa gcg gga ata ccg tgc cgc gtc agt ggt cgc gag aag cat ctt        720
Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu           
225                 230                 235                 240           

tat tcg att tac tgc aaa atg gtg ctc aaa gag cag cgt ttt cac tcg        768
Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe His Ser           
                245                 250                 255               

atc atg gac atc tac gct ttc cgc gtg atc gtc aat gat tct gac acc        816
Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Asn Asp Ser Asp Thr           
            260                 265                 270                   

tgt tat cgc gtg ctg ggc cag atg cac agc ctg tac aag ccg cgt ccg        864
Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro Arg Pro           
        275                 280                 285                       

ggc cgc gtg aaa gac tat atc gcc att cca aaa gcg aac ggc tat cag        912
Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln           
    290                 295                 300                           

tct ttg cac acc tcg atg atc ggc ccg cac ggt gtg ccg gtt gag gtc        960
Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val Glu Val           
305                 310                 315                 320           

cag atc cgt acc gaa gat atg gac cag atg gcg gag atg ggt gtt gcc       1008
Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala           
                325                 330                 335               

gcg cac tgg gct tat aaa gag cac ggc gaa acc agt act acc gca caa       1056
Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr Ala Gln           
            340                 345                 350                   

atc cgc gcc cag cgc tgg atg caa agc ctg ctg gag ctg caa cag agc       1104
Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln Gln Ser           
        355                 360                 365                       

gcc ggt agt tcg ttt gaa ttt atc gag agc gtt aaa tcc gat ctc ttc       1152
Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp Leu Phe           
    370                 375                 380                           

ccg gat gag att tac gtt ttc aca ccg gaa ggg cgc att gtc gag ctg       1200
Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val Glu Leu           
385                 390                 395                 400           

cct gcc ggt gca acg ccc gtc gac ttc gct tat gca gtg cat acc gat       1248
Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His Thr Asp           
                405                 410                 415               

atc ggt cat gcc tgc gtg ggc gca cgc gtt gac cgc cag cct tac ccg       1296
Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro Tyr Pro           
            420                 425                 430                   

ctg tcg cag ccg ctt acc agc ggt caa acc gtt gaa atc att acc gct       1344
Leu Ser Gln Pro Leu Thr Ser Gly Gln Thr Val Glu Ile Ile Thr Ala           
        435                 440                 445                       

ccg ggc gct cgc ccg aat gcc gct tgg ctg aac ttt gtc gtt agc tcg       1392
Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val Ser Ser           
    450                 455                 460                           

aaa gcg cgc gcc aaa att cgt cag ttg ctg aaa aac ctc aag cgt gat       1440
Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys Arg Asp           
465                 470                 475                 480           

gat tct gta agc ctg ggc cgt cgt ctg ctc aac cat gct ttg ggt ggt       1488
Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu Gly Gly           
                485                 490                 495               

agc cgt aag ctg aat gaa atc ccg cag gaa aat att cag cgc gag ctg       1536
Ser Arg Lys Leu Asn Glu Ile Pro Gln Glu Asn Ile Gln Arg Glu Leu           
            500                 505                 510                   

gat cgc atg aag ctg gca acg ctt gac gat ctg ctg gca gaa atc gga       1584
Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu Ile Gly           
        515                 520                 525                       

ctt ggt aac gca atg agc gtg gtg gtc gcg aaa aat ctg caa cat ggg       1632
Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln His Gly           
    530                 535                 540                           

gac gcc tcc att cca ccg gca acc caa agc cac gga cat ctg ccc att       1680
Asp Ala Ser Ile Pro Pro Ala Thr Gln Ser His Gly His Leu Pro Ile           
545                 550                 555                 560           

aaa ggt gcc gat ggc gtg ctg atc acc ttt gcg aaa tgc tgc cgc cct       1728
Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg Pro           
                565                 570                 575               

att cct ggc gac ccg att atc gcc cac gtc agc ccc ggt aaa ggt ctg       1776
Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly Leu           
            580                 585                 590                   

gtg atc cac cat gaa tcc tgc cgt aat atc cgt ggc tac cag aaa gag       1824
Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys Glu           
        595                 600                 605                       

cca gag aag ttt atg gct gtg gaa tgg gat aaa gag acg gcg cag gag       1872
Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Ala Gln Glu           
    610                 615                 620                           

ttc atc acc gaa atc aag gtg gag atg ttc aat cat cag ggt gcg ctg       1920
Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly Ala Leu           
625                 630                 635                 640           

gca aac ctg acg gcg gca att aac acc acg act tcg aat att caa agt       1968
Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile Gln Ser           
                645                 650                 655               

ttg aat acg gaa gag aaa gat ggt cgc gtc tac agc gcc ttt att cgt       2016
Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe Ile Arg           
            660                 665                 670                   

ctg acc gct cgt gac cgt gtg cat ctg gcg aat atc atg cgc aaa atc       2064
Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg Lys Ile           
        675                 680                 685                       

cgc gtg atg cca gac gtg att aaa gtc acc cga aac cga aat taa           2109
Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn                   
    690                 695                 700                           


<210>  2
<211>  702
<212>  PRT
<213>  Escherichia coli str. K12 substr. MG1655

<400>  2

Leu Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro 
1               5                   10                  15      


Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp 
            20                  25                  30          


Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His 
        35                  40                  45              


Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu 
    50                  55                  60                  


Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala 
65                  70                  75                  80  


Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu 
                85                  90                  95      


Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp Lys Lys 
            100                 105                 110         


Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln 
        115                 120                 125             


Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met 
    130                 135                 140                 


Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg 
145                 150                 155                 160 


Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His 
                165                 170                 175     


His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro 
            180                 185                 190         


Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn 
        195                 200                 205             


Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu 
    210                 215                 220                 


Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu 
225                 230                 235                 240 


Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe His Ser 
                245                 250                 255     


Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Asn Asp Ser Asp Thr 
            260                 265                 270         


Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro Arg Pro 
        275                 280                 285             


Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln 
    290                 295                 300                 


Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val Glu Val 
305                 310                 315                 320 


Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala 
                325                 330                 335     


Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr Ala Gln 
            340                 345                 350         


Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln Gln Ser 
        355                 360                 365             


Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp Leu Phe 
    370                 375                 380                 


Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val Glu Leu 
385                 390                 395                 400 


Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His Thr Asp 
                405                 410                 415     


Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro Tyr Pro 
            420                 425                 430         


Leu Ser Gln Pro Leu Thr Ser Gly Gln Thr Val Glu Ile Ile Thr Ala 
        435                 440                 445             


Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val Ser Ser 
    450                 455                 460                 


Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys Arg Asp 
465                 470                 475                 480 


Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu Gly Gly 
                485                 490                 495     


Ser Arg Lys Leu Asn Glu Ile Pro Gln Glu Asn Ile Gln Arg Glu Leu 
            500                 505                 510         


Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu Ile Gly 
        515                 520                 525             


Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln His Gly 
    530                 535                 540                 


Asp Ala Ser Ile Pro Pro Ala Thr Gln Ser His Gly His Leu Pro Ile 
545                 550                 555                 560 


Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg Pro 
                565                 570                 575     


Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly Leu 
            580                 585                 590         


Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys Glu 
        595                 600                 605             


Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Ala Gln Glu 
    610                 615                 620                 


Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly Ala Leu 
625                 630                 635                 640 


Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile Gln Ser 
                645                 650                 655     


Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe Ile Arg 
            660                 665                 670         


Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg Lys Ile 
        675                 680                 685             


Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn 
    690                 695                 700         


<210>  3
<211>  2112
<212>  DNA
<213>  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2


<220>
<221>  CDS
<222>  (1)..(2109)
<223>  spoT_St (Accession  NC_003197 REGION: 3934070..3936181; locus 
       tag: STM3742)

<400>  3
ttg tat ctg ttt gaa agc ctg aat caa ctg att caa acc tac ctg ccg         48
Leu Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro           
1               5                   10                  15                

gaa gac cag att aag cgt ctt cgg cag gcg tat ctc gtt gca cgt gac         96
Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp           
            20                  25                  30                    

gct cac gag ggc cag aca cgt tca agc ggt gaa ccc tat atc acg cac        144
Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His           
        35                  40                  45                        

ccg gtg gcg gtg gcc tgt att ctg gcc gag atg aaa ctc gac tac gaa        192
Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu           
    50                  55                  60                            

acg ctg atg gcc gct ctg ctg cat gac gtg att gaa gat acc ccc gcc        240
Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala           
65                  70                  75                  80            

acc tat cag gac atg gaa cag ctt ttc ggt aaa agc gtt gcc gag ctg        288
Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu           
                85                  90                  95                

gta gag ggg gtg tcg aaa ctt gat aag ctc aag ttt cgc gat aag aaa        336
Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp Lys Lys           
            100                 105                 110                   

gag gcg cag gcc gaa aac ttt cgc aaa atg att atg gcg atg gtg cag        384
Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln           
        115                 120                 125                       

gat atc cgc gtc atc ctc att aag ctt gct gac cgt acc cat aac atg        432
Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met           
    130                 135                 140                           

cgc acg ctg ggc tcg tta cgc ccg gat aaa cgt cgt cgt att gcc cgt        480
Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg           
145                 150                 155                 160           

gaa acg ctg gaa atc tac agt cct ctg gcg cac cgt tta ggt att cat        528
Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His           
                165                 170                 175               

cac atc aaa acc gag ctg gaa gag ctg ggt ttt gaa gcg ctg tat ccc        576
His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro           
            180                 185                 190                   

aat cgt tac cgc gtc atc aaa gaa gtg gta aaa gcg gcg cgc ggc aac        624
Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn           
        195                 200                 205                       

cgt aag gag atg atc caa aaa atc ctc tct gaa atc gaa gga cgt ttg        672
Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu           
    210                 215                 220                           

caa gag gcg gga att ccg tgt cgc gtt agc ggt cgc gaa aaa cat ctt        720
Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu           
225                 230                 235                 240           

tac tcg atc tac tgc aaa atg gtg ctc aaa gag cag cgt ttt cac tcg        768
Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe His Ser           
                245                 250                 255               

atc atg gac att tac gct ttc cgc gtc atc gtt cat gac tcc gat acc        816
Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val His Asp Ser Asp Thr           
            260                 265                 270                   

tgc tat cgc gta ctc ggc cag atg cac agt ctc tat aag ccg cgt ccg        864
Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro Arg Pro           
        275                 280                 285                       

gga cgg gtg aaa gac tat att gcc att ccc aaa gcg aac ggc tat cag        912
Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln           
    290                 295                 300                           

tct ttg cac acc tca atg atc ggc ccg cac ggc gtt cct gtt gaa gtc        960
Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val Glu Val           
305                 310                 315                 320           

cag atc cgt acc gaa gat atg gat cag atg gcg gaa atg ggg gtc gcg       1008
Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala           
                325                 330                 335               

gcg cac tgg gcg tat aaa gaa cac ggt gag acc agc acc acg gcg cag       1056
Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr Ala Gln           
            340                 345                 350                   

atc cgc gcc cag cgc tgg atg cag agc ctg ctg gag cta caa cag agc       1104
Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln Gln Ser           
        355                 360                 365                       

gcc ggt agt tcg ttt gaa ttt atc gaa agc gta aaa tcc gat ctc ttc       1152
Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp Leu Phe           
    370                 375                 380                           

ccg gat gag att tac gtt ttc acc ccg gaa ggg cgc att gtc gaa ctg       1200
Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val Glu Leu           
385                 390                 395                 400           

ccc gct ggc gct acg ccg gtg gat ttt gcc tat gca gtg cat acc gac       1248
Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His Thr Asp           
                405                 410                 415               

atc ggc cac gcc tgc gtc ggc gcg cgt gtc gac cgc cag cct tat ccg       1296
Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro Tyr Pro           
            420                 425                 430                   

ctg tcg cag ccg ctt agc agc ggt cag acc gtc gaa att att acc gcg       1344
Leu Ser Gln Pro Leu Ser Ser Gly Gln Thr Val Glu Ile Ile Thr Ala           
        435                 440                 445                       

ccg ggc gcg cgt ccc aac gcc gcc tgg ctg aac ttt gtc gtc agc tct       1392
Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val Ser Ser           
    450                 455                 460                           

aaa gcg cgc gct aaa att cgt cag ttg ctg aaa aac ctc aaa cgt gat       1440
Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys Arg Asp           
465                 470                 475                 480           

gac tcc gta agc ctg ggc cgt cgt ctg ctt aac cat gcc tta ggc ggt       1488
Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu Gly Gly           
                485                 490                 495               

agt cgt aag ctg gcg gaa att ccg cag gaa aat att cag cgc gaa ttg       1536
Ser Arg Lys Leu Ala Glu Ile Pro Gln Glu Asn Ile Gln Arg Glu Leu           
            500                 505                 510                   

gat cgt atg aag ctg gca acg ctt gac gat ctg ctg gcg gaa atc ggt       1584
Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu Ile Gly           
        515                 520                 525                       

ctc ggc aac gcg atg agc gta gtg gtc gcg aaa aat ctg cag caa ggc       1632
Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln Gln Gly           
    530                 535                 540                           

gaa gcc gtg gtg ccg acc gtt gcg caa tcg aat cac ggc cac ctg ccg       1680
Glu Ala Val Val Pro Thr Val Ala Gln Ser Asn His Gly His Leu Pro           
545                 550                 555                 560           

att aaa ggc gcg gat ggc gtg ctt atc acc ttt gcg aag tgt tgt cgt       1728
Ile Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg           
                565                 570                 575               

ccg atc cca ggc gac ccg atc atc gct cac gtc agc cca ggt aaa gga       1776
Pro Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly           
            580                 585                 590                   

ctg gtg atc cac cac gaa tcc tgc cgt aat atc cgt gga tac cag aaa       1824
Leu Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys           
        595                 600                 605                       

gag cca gag aaa ttt atg gcg gtc gaa tgg gac aaa gag acg gag cag       1872
Glu Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Glu Gln           
    610                 615                 620                           

gaa ttc att acc gaa atc aag gtg gaa atg ttt aac cat cag ggc gcg       1920
Glu Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly Ala           
625                 630                 635                 640           

ctg gct aac ctg acg gcg gcg att aat acc acc acc tcc aat att caa       1968
Leu Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile Gln           
                645                 650                 655               

agc ctg aat act gaa gag aaa gat ggt cgc gtc tat agt acc ttt att       2016
Ser Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Thr Phe Ile           
            660                 665                 670                   

cgc ctt acc gca cgc gat cgc gta cat ctg gcg aat atc atg cgc aaa       2064
Arg Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg Lys           
        675                 680                 685                       

atc cgc gtg atg cca gac gtc att aaa gtc acc cgt aac cga aac tag       2112
Ile Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn               
    690                 695                 700                           


<210>  4
<211>  703
<212>  PRT
<213>  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

<400>  4

Leu Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro 
1               5                   10                  15      


Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp 
            20                  25                  30          


Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His 
        35                  40                  45              


Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu 
    50                  55                  60                  


Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala 
65                  70                  75                  80  


Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu 
                85                  90                  95      


Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp Lys Lys 
            100                 105                 110         


Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln 
        115                 120                 125             


Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met 
    130                 135                 140                 


Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg 
145                 150                 155                 160 


Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His 
                165                 170                 175     


His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro 
            180                 185                 190         


Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn 
        195                 200                 205             


Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu 
    210                 215                 220                 


Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu 
225                 230                 235                 240 


Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe His Ser 
                245                 250                 255     


Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val His Asp Ser Asp Thr 
            260                 265                 270         


Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro Arg Pro 
        275                 280                 285             


Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln 
    290                 295                 300                 


Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val Glu Val 
305                 310                 315                 320 


Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala 
                325                 330                 335     


Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr Ala Gln 
            340                 345                 350         


Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln Gln Ser 
        355                 360                 365             


Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp Leu Phe 
    370                 375                 380                 


Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val Glu Leu 
385                 390                 395                 400 


Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His Thr Asp 
                405                 410                 415     


Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro Tyr Pro 
            420                 425                 430         


Leu Ser Gln Pro Leu Ser Ser Gly Gln Thr Val Glu Ile Ile Thr Ala 
        435                 440                 445             


Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val Ser Ser 
    450                 455                 460                 


Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys Arg Asp 
465                 470                 475                 480 


Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu Gly Gly 
                485                 490                 495     


Ser Arg Lys Leu Ala Glu Ile Pro Gln Glu Asn Ile Gln Arg Glu Leu 
            500                 505                 510         


Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu Ile Gly 
        515                 520                 525             


Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln Gln Gly 
    530                 535                 540                 


Glu Ala Val Val Pro Thr Val Ala Gln Ser Asn His Gly His Leu Pro 
545                 550                 555                 560 


Ile Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg 
                565                 570                 575     


Pro Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly 
            580                 585                 590         


Leu Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys 
        595                 600                 605             


Glu Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Glu Gln 
    610                 615                 620                 


Glu Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly Ala 
625                 630                 635                 640 


Leu Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile Gln 
                645                 650                 655     


Ser Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Thr Phe Ile 
            660                 665                 670         


Arg Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg Lys 
        675                 680                 685             


Ile Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn 
    690                 695                 700             


<210>  5
<211>  2112
<212>  DNA
<213>  Serratia proteamaculans 568


<220>
<221>  CDS
<222>  (1)..(2109)
<223>  locus tag Spro_4869

<400>  5
ttg tac ctg ttt gaa agc ctg aat ctg ctg att caa cgt tac ctg cct         48
Leu Tyr Leu Phe Glu Ser Leu Asn Leu Leu Ile Gln Arg Tyr Leu Pro           
1               5                   10                  15                

gag gag cag att aag cgc ctc aaa cag gca tac ctc gtt gca cgt gat         96
Glu Glu Gln Ile Lys Arg Leu Lys Gln Ala Tyr Leu Val Ala Arg Asp           
            20                  25                  30                    

gct cac gag gga cag aca cgc tcc agc ggt gag ccc tac att act cac        144
Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His           
        35                  40                  45                        

ccg gtt gcc gtg gcc tgc att ctg gcg gaa atg cgt ctc gat cat gag        192
Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Arg Leu Asp His Glu           
    50                  55                  60                            

acg ctg atg gca gca ctg ctg cac gac gtc atc gaa gat acc ccg gcc        240
Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala           
65                  70                  75                  80            

acc tac cag gat atg gaa cag cta ttc ggc aaa agc gtt gcc gaa ctg        288
Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu           
                85                  90                  95                

gtt gaa ggc gta tcc aag ctc gac aag ctg aag ttc cag gat aaa aaa        336
Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Gln Asp Lys Lys           
            100                 105                 110                   

gaa gct cag gcg gaa aac ttc cgc aag atg atc atg gcg atg gtg cag        384
Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln           
        115                 120                 125                       

gat atc cgc gtc gtg ctg atc aag ctg gcc gac cgt acg cac aac atg        432
Asp Ile Arg Val Val Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met           
    130                 135                 140                           

cgc act ctc ggt tcg ctg cgt cct gac aaa cgg cgg cgt att gca cgt        480
Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg           
145                 150                 155                 160           

gaa acc ctg gaa atc tac agc ccc ctg gcc cat cgc ctg ggt att cac        528
Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His           
                165                 170                 175               

cac ctg aaa acc gag ctg gaa gag ctg ggt ttt gag gcg ttg tac ccg        576
His Leu Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro           
            180                 185                 190                   

aac cgc tat cgc gta atc aaa gaa gtg gtg aaa gcc gca cgc ggt aac        624
Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn           
        195                 200                 205                       

cgt aaa gag atg atc cag aag att ctc tcc gag atc gaa ggg cga ctg        672
Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu           
    210                 215                 220                           

acc gag gcc ggc att ccc tgc cgc gtc agc gga cgg gaa aaa cat ctg        720
Thr Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu           
225                 230                 235                 240           

tat tcc atc tac ctc aag atg cac ctg aaa gaa cag cgt ttc cat tcg        768
Tyr Ser Ile Tyr Leu Lys Met His Leu Lys Glu Gln Arg Phe His Ser           
                245                 250                 255               

att atg gat atc tac gcg ttc cgg gtg atc gtg aag gaa gtc gac acc        816
Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Lys Glu Val Asp Thr           
            260                 265                 270                   

tgc tac cgc gtg ctg ggc cag gct cac agc ctg tac aaa ccg cgt ccg        864
Cys Tyr Arg Val Leu Gly Gln Ala His Ser Leu Tyr Lys Pro Arg Pro           
        275                 280                 285                       

ggc agg gtc aaa gac tat atc gcc att ccc aag gcc aac ggc tat caa        912
Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln           
    290                 295                 300                           

tcg ctg cac acc tca ctg atc ggc cca cat ggc gta ccg gtt gaa gtg        960
Ser Leu His Thr Ser Leu Ile Gly Pro His Gly Val Pro Val Glu Val           
305                 310                 315                 320           

cag atc cgt acc gaa gat atg gat cag atg gcc gaa atg ggg gtc gcc       1008
Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala           
                325                 330                 335               

gcg cac tgg gct tat aaa gaa aaa gaa cag ggc gaa acc ggc acc acc       1056
Ala His Trp Ala Tyr Lys Glu Lys Glu Gln Gly Glu Thr Gly Thr Thr           
            340                 345                 350                   

gca caa atc cgc gct cag cgg tgg atg cag agc ttg ctg gag ctg caa       1104
Ala Gln Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln           
        355                 360                 365                       

caa agc gcc ggc agt tcg ttt gaa ttt atc gag agc gta aaa tcc gat       1152
Gln Ser Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp           
    370                 375                 380                           

ctg ttc ccg gat gag att tac gtt ttc acc ccg gaa ggc cgc att gtt       1200
Leu Phe Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val           
385                 390                 395                 400           

gaa ttg cct gcg ggc gca acg ccg gtc gac ttc gcc tac gcg gtg cac       1248
Glu Leu Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His           
                405                 410                 415               

acc gat atc ggc cac gcc tgc gtt ggc gca cgt gtt gat cgc cag cca       1296
Thr Asp Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro           
            420                 425                 430                   

tac ccg ctg tca cag tcc ttg acc agc ggg caa acg gtc gaa atc atc       1344
Tyr Pro Leu Ser Gln Ser Leu Thr Ser Gly Gln Thr Val Glu Ile Ile           
        435                 440                 445                       

acg gct cct ggt gca cgg cct aac gcc gcc tgg ctg aac ttt gtc gtc       1392
Thr Ala Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val           
    450                 455                 460                           

agc tca aaa gcg cgc gca aaa atc cgc caa atg ctg aaa aac ctc aag       1440
Ser Ser Lys Ala Arg Ala Lys Ile Arg Gln Met Leu Lys Asn Leu Lys           
465                 470                 475                 480           

cgc gat gat tca gtc ggc ctc ggc cgc cgc ttg tta aat cat gca ttg       1488
Arg Asp Asp Ser Val Gly Leu Gly Arg Arg Leu Leu Asn His Ala Leu           
                485                 490                 495               

ggc ggc agt cgc aaa ctg gca gag gtt ccg gca gaa aat atc caa cac       1536
Gly Gly Ser Arg Lys Leu Ala Glu Val Pro Ala Glu Asn Ile Gln His           
            500                 505                 510                   

gag ttg gat cgc atg aaa ctg gcg acg ctg gac gac ctg ttg gct gaa       1584
Glu Leu Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu           
        515                 520                 525                       

atc ggc ttg ggc aat gcc atg agc gtg gtg gta gcg aaa aac ctg caa       1632
Ile Gly Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln           
    530                 535                 540                           

ggt gac cag tct aat ctg ggg gcc tcc tct ggg gtt cgt aat ctg gcg       1680
Gly Asp Gln Ser Asn Leu Gly Ala Ser Ser Gly Val Arg Asn Leu Ala           
545                 550                 555                 560           

atc aag ggc tca gac ggc gtg ctg atc acc ttc gct aaa tgc tgc cga       1728
Ile Lys Gly Ser Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg           
                565                 570                 575               

cct att cca ggc gat ccg atc atc gcc cac gtc agc cca ggc aaa ggc       1776
Pro Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly           
            580                 585                 590                   

ctg gtg atc cac cat gag tcc tgc cga aat atc cgc ggc tac cag aaa       1824
Leu Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys           
        595                 600                 605                       

gaa cct gaa aaa ttc atg gcg gta gag tgg gat caa gac atc gaa cag       1872
Glu Pro Glu Lys Phe Met Ala Val Glu Trp Asp Gln Asp Ile Glu Gln           
    610                 615                 620                           

gaa ttc atc gcc gag atc aaa gtg gac atg ttt aat cat cag ggg gca       1920
Glu Phe Ile Ala Glu Ile Lys Val Asp Met Phe Asn His Gln Gly Ala           
625                 630                 635                 640           

ctg gcc aac ctg acg gca gcc atc aat gcg gct gag tcc aat att caa       1968
Leu Ala Asn Leu Thr Ala Ala Ile Asn Ala Ala Glu Ser Asn Ile Gln           
                645                 650                 655               

agc ctg aat acc gaa gaa aaa gac ggc cgg gtt tat agt gca ttt atc       2016
Ser Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe Ile           
            660                 665                 670                   

cgt ttg acc acc cgc gat cgc atc cat ctg gca aat att atg cgt aaa       2064
Arg Leu Thr Thr Arg Asp Arg Ile His Leu Ala Asn Ile Met Arg Lys           
        675                 680                 685                       

atc cgt atc atg ccg gat gtg att aaa gtt aac cgt aac cga aat tag       2112
Ile Arg Ile Met Pro Asp Val Ile Lys Val Asn Arg Asn Arg Asn               
    690                 695                 700                           


<210>  6
<211>  703
<212>  PRT
<213>  Serratia proteamaculans 568

<400>  6

Leu Tyr Leu Phe Glu Ser Leu Asn Leu Leu Ile Gln Arg Tyr Leu Pro 
1               5                   10                  15      


Glu Glu Gln Ile Lys Arg Leu Lys Gln Ala Tyr Leu Val Ala Arg Asp 
            20                  25                  30          


Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His 
        35                  40                  45              


Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Arg Leu Asp His Glu 
    50                  55                  60                  


Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala 
65                  70                  75                  80  


Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu 
                85                  90                  95      


Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Gln Asp Lys Lys 
            100                 105                 110         


Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln 
        115                 120                 125             


Asp Ile Arg Val Val Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met 
    130                 135                 140                 


Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg 
145                 150                 155                 160 


Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His 
                165                 170                 175     


His Leu Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro 
            180                 185                 190         


Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn 
        195                 200                 205             


Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu 
    210                 215                 220                 


Thr Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu 
225                 230                 235                 240 


Tyr Ser Ile Tyr Leu Lys Met His Leu Lys Glu Gln Arg Phe His Ser 
                245                 250                 255     


Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Lys Glu Val Asp Thr 
            260                 265                 270         


Cys Tyr Arg Val Leu Gly Gln Ala His Ser Leu Tyr Lys Pro Arg Pro 
        275                 280                 285             


Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln 
    290                 295                 300                 


Ser Leu His Thr Ser Leu Ile Gly Pro His Gly Val Pro Val Glu Val 
305                 310                 315                 320 


Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala 
                325                 330                 335     


Ala His Trp Ala Tyr Lys Glu Lys Glu Gln Gly Glu Thr Gly Thr Thr 
            340                 345                 350         


Ala Gln Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln 
        355                 360                 365             


Gln Ser Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp 
    370                 375                 380                 


Leu Phe Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val 
385                 390                 395                 400 


Glu Leu Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His 
                405                 410                 415     


Thr Asp Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro 
            420                 425                 430         


Tyr Pro Leu Ser Gln Ser Leu Thr Ser Gly Gln Thr Val Glu Ile Ile 
        435                 440                 445             


Thr Ala Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val 
    450                 455                 460                 


Ser Ser Lys Ala Arg Ala Lys Ile Arg Gln Met Leu Lys Asn Leu Lys 
465                 470                 475                 480 


Arg Asp Asp Ser Val Gly Leu Gly Arg Arg Leu Leu Asn His Ala Leu 
                485                 490                 495     


Gly Gly Ser Arg Lys Leu Ala Glu Val Pro Ala Glu Asn Ile Gln His 
            500                 505                 510         


Glu Leu Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu 
        515                 520                 525             


Ile Gly Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln 
    530                 535                 540                 


Gly Asp Gln Ser Asn Leu Gly Ala Ser Ser Gly Val Arg Asn Leu Ala 
545                 550                 555                 560 


Ile Lys Gly Ser Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg 
                565                 570                 575     


Pro Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly 
            580                 585                 590         


Leu Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys 
        595                 600                 605             


Glu Pro Glu Lys Phe Met Ala Val Glu Trp Asp Gln Asp Ile Glu Gln 
    610                 615                 620                 


Glu Phe Ile Ala Glu Ile Lys Val Asp Met Phe Asn His Gln Gly Ala 
625                 630                 635                 640 


Leu Ala Asn Leu Thr Ala Ala Ile Asn Ala Ala Glu Ser Asn Ile Gln 
                645                 650                 655     


Ser Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe Ile 
            660                 665                 670         


Arg Leu Thr Thr Arg Asp Arg Ile His Leu Ala Asn Ile Met Arg Lys 
        675                 680                 685             


Ile Arg Ile Met Pro Asp Val Ile Lys Val Asn Arg Asn Arg Asn 
    690                 695                 700             


<210>  7
<211>  2115
<212>  DNA
<213>  Escherichia coli str. K12 substr. MG442


<220>
<221>  CDS
<222>  (1)..(2112)
<223>  spoT_allel

<220>
<221>  mutation
<222>  (253)..(258)
<223>  Insertion of catgat causes an HD-Insertion after amino acid 
       position 84 in the SpoT_allel protein

<220>
<221>  mutation
<222>  (526)..(526)
<223>  SNP c520t  (position referred to spoT_MG1655) causes the amino 
       acid exchange G174C in the SpoT_allel protein (position referred 
       to SpoT_MG1655)

<220>
<221>  mutation
<222>  (1591)..(1591)
<223>  SNP c1585t (position referred to spoT_MG1655) causes the amino 
       acid exchange L529F in the SpoT_allel protein (position referred 
       to SpoT_MG1655)

<400>  7
ttg tat ctg ttt gaa agc ctg aat caa ctg att caa acc tac ctg ccg         48
Leu Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro           
1               5                   10                  15                

gaa gac caa atc aag cgt ctg cgg cag gcg tat ctc gtt gca cgt gat         96
Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp           
            20                  25                  30                    

gct cac gag ggg caa aca cgt tca agc ggt gaa ccc tat atc acg cac        144
Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His           
        35                  40                  45                        

ccg gta gcg gtt gcc tgc att ctg gcc gag atg aaa ctc gac tat gaa        192
Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu           
    50                  55                  60                            

acg ctg atg gcg gcg ctg ctg cat gac gtg att gaa gat act ccc gcc        240
Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala           
65                  70                  75                  80            

acc tac cag gat cat gat atg gaa cag ctt ttt ggt aaa agc gtc gcc        288
Thr Tyr Gln Asp His Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala           
                85                  90                  95                

gag ctg gta gag ggg gtg tcg aaa ctt gat aaa ctc aag ttc cgc gat        336
Glu Leu Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp           
            100                 105                 110                   

aag aaa gag gcg cag gcc gaa aac ttt cgc aag atg att atg gcg atg        384
Lys Lys Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met           
        115                 120                 125                       

gtg cag gat atc cgc gtc atc ctc atc aaa ctt gcc gac cgt acc cac        432
Val Gln Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His           
    130                 135                 140                           

aac atg cgc acg ctg ggc tca ctt cgc ccg gac aaa cgt cgc cgc atc        480
Asn Met Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile           
145                 150                 155                 160           

gcc cgt gaa act ctc gaa att tat agc ccg ctg gcg cac cgt tta tgt        528
Ala Arg Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Cys           
                165                 170                 175               

atc cac cac att aaa acc gaa ctc gaa gag ctg ggt ttt gag gcg ctg        576
Ile His His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu           
            180                 185                 190                   

tat ccc aac cgt tat cgc gta atc aaa gaa gtg gtg aaa gcc gcg cgc        624
Tyr Pro Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg           
        195                 200                 205                       

ggc aac cgt aaa gag atg atc cag aag att ctt tct gaa atc gaa ggg        672
Gly Asn Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly           
    210                 215                 220                           

cgt ttg cag gaa gcg gga ata ccg tgc cgc gtc agt ggt cgc gag aag        720
Arg Leu Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys           
225                 230                 235                 240           

cat ctt tat tcg att tac tgc aaa atg gtg ctc aaa gag cag cgt ttt        768
His Leu Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe           
                245                 250                 255               

cac tcg atc atg gac atc tac gct ttc cgc gtg atc gtc aat gat tct        816
His Ser Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Asn Asp Ser           
            260                 265                 270                   

gac acc tgt tat cgc gtg ctg ggc cag atg cac agc ctg tac aag ccg        864
Asp Thr Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro           
        275                 280                 285                       

cgt ccg ggc cgc gtg aaa gac tat atc gcc att cca aaa gcg aac ggc        912
Arg Pro Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly           
    290                 295                 300                           

tat cag tct ttg cac acc tcg atg atc ggc ccg cac ggt gtg ccg gtt        960
Tyr Gln Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val           
305                 310                 315                 320           

gag gtc cag atc cgt acc gaa gat atg gac cag atg gcg gag atg ggt       1008
Glu Val Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly           
                325                 330                 335               

gtt gcc gcg cac tgg gct tat aaa gag cac ggc gaa acc agt act acc       1056
Val Ala Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr           
            340                 345                 350                   

gca caa atc cgc gcc cag cgc tgg atg caa agc ctg ctg gag ctg caa       1104
Ala Gln Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln           
        355                 360                 365                       

cag agc gcc ggt agt tcg ttt gaa ttt atc gag agc gtt aaa tcc gat       1152
Gln Ser Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp           
    370                 375                 380                           

ctc ttc ccg gat gag att tac gtt ttc aca ccg gaa ggg cgc att gtc       1200
Leu Phe Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val           
385                 390                 395                 400           

gag ctg cct gcc ggt gca acg ccc gtc gac ttc gct tat gca gtg cat       1248
Glu Leu Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His           
                405                 410                 415               

acc gat atc ggt cat gcc tgc gtg ggc gca cgc gtt gac cgc cag cct       1296
Thr Asp Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro           
            420                 425                 430                   

tac ccg ctg tcg cag ccg ctt acc agc ggt caa acc gtt gaa atc att       1344
Tyr Pro Leu Ser Gln Pro Leu Thr Ser Gly Gln Thr Val Glu Ile Ile           
        435                 440                 445                       

acc gct ccg ggc gct cgc ccg aat gcc gct tgg ctg aac ttt gtc gtt       1392
Thr Ala Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val           
    450                 455                 460                           

agc tcg aaa gcg cgc gcc aaa att cgt cag ttg ctg aaa aac ctc aag       1440
Ser Ser Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys           
465                 470                 475                 480           

cgt gat gat tct gta agc ctg ggc cgt cgt ctg ctc aac cat gct ttg       1488
Arg Asp Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu           
                485                 490                 495               

ggt ggt agc cgt aag ctg aat gaa atc ccg cag gaa aat att cag cgc       1536
Gly Gly Ser Arg Lys Leu Asn Glu Ile Pro Gln Glu Asn Ile Gln Arg           
            500                 505                 510                   

gag ctg gat cgc atg aag ctg gca acg ctt gac gat ctg ctg gca gaa       1584
Glu Leu Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu           
        515                 520                 525                       

atc gga ttt ggt aac gca atg agc gtg gtg gtc gcg aaa aat ctg caa       1632
Ile Gly Phe Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln           
    530                 535                 540                           

cat ggg gac gcc tcc att cca ccg gca acc caa agc cac gga cat ctg       1680
His Gly Asp Ala Ser Ile Pro Pro Ala Thr Gln Ser His Gly His Leu           
545                 550                 555                 560           

ccc att aaa ggt gcc gat ggc gtg ctg atc acc ttt gcg aaa tgc tgc       1728
Pro Ile Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys           
                565                 570                 575               

cgc cct att cct ggc gac ccg att atc gcc cac gtc agc ccc ggt aaa       1776
Arg Pro Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys           
            580                 585                 590                   

ggt ctg gtg atc cac cat gaa tcc tgc cgt aat atc cgt ggc tac cag       1824
Gly Leu Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln           
        595                 600                 605                       

aaa gag cca gag aag ttt atg gct gtg gaa tgg gat aaa gag acg gcg       1872
Lys Glu Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Ala           
    610                 615                 620                           

cag gag ttc atc acc gaa atc aag gtg gag atg ttc aat cat cag ggt       1920
Gln Glu Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly           
625                 630                 635                 640           

gcg ctg gca aac ctg acg gcg gca att aac acc acg act tcg aat att       1968
Ala Leu Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile           
                645                 650                 655               

caa agt ttg aat acg gaa gag aaa gat ggt cgc gtc tac agc gcc ttt       2016
Gln Ser Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe           
            660                 665                 670                   

att cgt ctg acc gct cgt gac cgt gtg cat ctg gcg aat atc atg cgc       2064
Ile Arg Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg           
        675                 680                 685                       

aaa atc cgc gtg atg cca gac gtg att aaa gtc acc cga aac cga aat       2112
Lys Ile Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn           
    690                 695                 700                           

taa                                                                   2115


<210>  8
<211>  704
<212>  PRT
<213>  Escherichia coli str. K12 substr. MG442

<400>  8

Leu Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro 
1               5                   10                  15      


Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp 
            20                  25                  30          


Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His 
        35                  40                  45              


Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu 
    50                  55                  60                  


Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala 
65                  70                  75                  80  


Thr Tyr Gln Asp His Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala 
                85                  90                  95      


Glu Leu Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp 
            100                 105                 110         


Lys Lys Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met 
        115                 120                 125             


Val Gln Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His 
    130                 135                 140                 


Asn Met Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile 
145                 150                 155                 160 


Ala Arg Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Cys 
                165                 170                 175     


Ile His His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu 
            180                 185                 190         


Tyr Pro Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg 
        195                 200                 205             


Gly Asn Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly 
    210                 215                 220                 


Arg Leu Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys 
225                 230                 235                 240 


His Leu Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe 
                245                 250                 255     


His Ser Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Asn Asp Ser 
            260                 265                 270         


Asp Thr Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro 
        275                 280                 285             


Arg Pro Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly 
    290                 295                 300                 


Tyr Gln Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val 
305                 310                 315                 320 


Glu Val Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly 
                325                 330                 335     


Val Ala Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr 
            340                 345                 350         


Ala Gln Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln 
        355                 360                 365             


Gln Ser Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp 
    370                 375                 380                 


Leu Phe Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val 
385                 390                 395                 400 


Glu Leu Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His 
                405                 410                 415     


Thr Asp Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro 
            420                 425                 430         


Tyr Pro Leu Ser Gln Pro Leu Thr Ser Gly Gln Thr Val Glu Ile Ile 
        435                 440                 445             


Thr Ala Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val 
    450                 455                 460                 


Ser Ser Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys 
465                 470                 475                 480 


Arg Asp Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu 
                485                 490                 495     


Gly Gly Ser Arg Lys Leu Asn Glu Ile Pro Gln Glu Asn Ile Gln Arg 
            500                 505                 510         


Glu Leu Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu 
        515                 520                 525             


Ile Gly Phe Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln 
    530                 535                 540                 


His Gly Asp Ala Ser Ile Pro Pro Ala Thr Gln Ser His Gly His Leu 
545                 550                 555                 560 


Pro Ile Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys 
                565                 570                 575     


Arg Pro Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys 
            580                 585                 590         


Gly Leu Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln 
        595                 600                 605             


Lys Glu Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Ala 
    610                 615                 620                 


Gln Glu Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly 
625                 630                 635                 640 


Ala Leu Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile 
                645                 650                 655     


Gln Ser Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe 
            660                 665                 670         


Ile Arg Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg 
        675                 680                 685             


Lys Ile Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn 
    690                 695                 700                 


<210>  9
<211>  32
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  Primer ligB-up

<400>  9
cagtactcta gaagccacga aggacactaa gg                                     32


<210>  10
<211>  32
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  Primer ligB-down

<400>  10
ttagttggta cccggatgga ccgcagttaa tg                                     32


<210>  11
<211>  1045
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(1045)
<223>  PCR product ligB

<400>  11
cagtactcta gaagccacga aggacactaa ggttcaaaac ctgtgatctg ctgggcagcc       60

agccaactgc ccagcttctt gatttgcgca ttttccttcc attcaataac ctgtctggcg      120

cgtcccgatc cagtccccgg cagctgctgc cagaactgct ccgtgctaaa taaaagttgc      180

gaccaggacc gttcatcact ggcattaagc gccgcccggg ttagcggtat tcccattgcc      240

atcacccagc gagtaaaagg ctgcttacga gccagattaa actgatgcca tagctgcgca      300

cttttacttt tcgcgatccc cggcgtgttc tgtaattgct ctggcgttaa taaaagccag      360

gaaaagatat gttcaaagcg atgagtctga tgcagcgcgc gccaaccggc ctcaccaatg      420

ccatccagcc caagaacctg ttttgccccc agccagacta agcgtgaaat gaactgttcc      480

tgacaaacat cagaagcaaa gtagcaggtc aacgagttaa agcggttttc tggcggtgtc      540

ggttttgtac gttctgcacc gcgccacacc acatcatcaa tgcgaggaat accctgaccg      600

gcaaggctga cgagaatctg atcaccaggc gcaatatccc actcctgcca gcgcctgacg      660

gaaccaatat tcacccgctg gactttttta tcatccagca tgacaggtgc gagtgacgca      720

accaccgata ttttaccgct cttacccacc gcaaactgaa ttgccttcac ttcggcaacc      780

tgagctacag gttgatattt ccaggccacc agccactctg cctggcccgg tagccaatgg      840

cgggattctg gctctttcgc cgctcgtaca actacgccat cggtgacgaa gggtaattcc      900

gctttccacc actcattgcg tacgcgcgca acttcatcag catttttcac cgcacgggta      960

tacgtctgcg ttagagtaaa acctgcggta gccagctctt ttaaacgatc agacattaac     1020

tgcggtccat ccgggtacca actaa                                           1045


<210>  12
<211>  38
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  primer serCF(XbaI)

<400>  12
aggtgctcta gagtccgcgc tgtgcaaatc cagaatgg                               38


<210>  13
<211>  37
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(37)
<223>  primer serCR(HindIII)

<400>  13
tacaccaagc ttaactctct acaacagaaa taaaaac                                37


<210>  14
<211>  33
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  primer serAF(XbaI)

<400>  14
ctgtagtcta gattagtaca gcagacgggc gcg                                    33


<210>  15
<211>  68
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(68)
<223>  primer serAR(SHSSNB)

<400>  15
caagagctca agcttgcatg cgattcccgg gcggccgcaa taagatctcc gtcagggcgt       60

ggtgaccg                                                                68


<210>  16
<211>  34
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(34)
<223>  primer serB(SphI)

<400>  16
ccatgcgcat gcccaccctt tgaaaatttg agac                                   34


<210>  17
<211>  75
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  primer serB(SmaI)

<400>  17
ccgcatgtcg acatcccggg gcagaaaggc ccacccgaag gtgagccagt gtgattactt       60

ctgattcagg ctgcc                                                        75


<210>  18
<211>  35
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  primer glyA-downstream

<400>  18
atctaaagat ctgttacgac agatttgatg gcgcg                                  35


<210>  19
<211>  33
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  primer glyA-upstream

<400>  19
ttcatcgcgg ccgcgaaaga atgtgatgaa gtg                                    33


2
201100331

