                         SEQUENCE LISTING

<110>  Academia Sinica
 
<120>  ANTI-EPITHELIAL CELL ADHESION MOLECULE (EpCAM) ANTIBODIES AND 
       METHODS OF USE THEREOF

<130>  10011-00024

<150>  61/606,220
<151>  2012-03-02

<160>  63    

<170>  PatentIn version 3.5

<210>  1
<211>  314
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Pro Pro Gln Val Leu Ala Phe Gly Leu Leu Leu Ala Ala Ala 
1               5                   10                  15      


Thr Ala Thr Phe Ala Ala Ala Gln Glu Glu Cys Val Cys Glu Asn Tyr 
            20                  25                  30          


Lys Leu Ala Val Asn Cys Phe Val Asn Asn Asn Arg Gln Cys Gln Cys 
        35                  40                  45              


Thr Ser Val Gly Ala Gln Asn Thr Val Ile Cys Ser Lys Leu Ala Ala 
    50                  55                  60                  


Lys Cys Leu Val Met Lys Ala Glu Met Asn Gly Ser Lys Leu Gly Arg 
65                  70                  75                  80  


Arg Ala Lys Pro Glu Gly Ala Leu Gln Asn Asn Asp Gly Leu Tyr Asp 
                85                  90                  95      


Pro Asp Cys Asp Glu Ser Gly Leu Phe Lys Ala Lys Gln Cys Asn Gly 
            100                 105                 110         


Thr Ser Met Cys Trp Cys Val Asn Thr Ala Gly Val Arg Arg Thr Asp 
        115                 120                 125             


Lys Asp Thr Glu Ile Thr Cys Ser Glu Arg Val Arg Thr Tyr Trp Ile 
    130                 135                 140                 


Ile Ile Glu Leu Lys His Lys Ala Arg Glu Lys Pro Tyr Asp Ser Lys 
145                 150                 155                 160 


Ser Leu Arg Thr Ala Leu Gln Lys Glu Ile Thr Thr Arg Tyr Gln Leu 
                165                 170                 175     


Asp Pro Lys Phe Ile Thr Ser Ile Leu Tyr Glu Asn Asn Val Ile Thr 
            180                 185                 190         


Ile Asp Leu Val Gln Asn Ser Ser Gln Lys Thr Gln Asn Asp Val Asp 
        195                 200                 205             


Ile Ala Asp Val Ala Tyr Tyr Phe Glu Lys Asp Val Lys Gly Glu Ser 
    210                 215                 220                 


Leu Phe His Ser Lys Lys Met Asp Leu Thr Val Asn Gly Glu Gln Leu 
225                 230                 235                 240 


Asp Leu Asp Pro Gly Gln Thr Leu Ile Tyr Tyr Val Asp Glu Lys Ala 
                245                 250                 255     


Pro Glu Phe Ser Met Gln Gly Leu Lys Ala Gly Val Ile Ala Val Ile 
            260                 265                 270         


Val Val Val Val Ile Ala Val Val Ala Gly Ile Val Val Leu Val Ile 
        275                 280                 285             


Ser Arg Lys Lys Arg Met Ala Lys Tyr Glu Lys Ala Glu Ile Lys Glu 
    290                 295                 300                 


Met Gly Glu Met His Arg Glu Leu Asn Ala 
305                 310                 


<210>  2
<211>  113
<212>  PRT
<213>  Homo sapiens

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Ser Met His Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Phe Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Arg Thr Thr Tyr 
65                  70                  75                  80  


Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Thr Ala Val Tyr Trp Gly Gln Gly Thr Ser Leu Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  3
<211>  116
<212>  PRT
<213>  Homo sapiens

<400>  3

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys Arg Ala Ser Gln Glu Ile Ser Val Ser 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Asp Gly Thr Ile Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Thr Ser Thr Leu Asp Ser Gly Val Pro Lys Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Leu Gln Tyr Ala Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser 
        115     


<210>  4
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  4

Gly Tyr Thr Phe Thr Asp Tyr Ser Met His 
1               5                   10  


<210>  5
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  5

Ile Asn Thr Glu Thr Gly Glu Pro 
1               5               


<210>  6
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  6

Thr Ala Val Tyr 
1               


<210>  7
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  7

Arg Ala Ser Gln Glu Ile Ser Val Ser Leu Ser 
1               5                   10      


<210>  8
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  8

Ala Thr Ser Thr Leu Asp Ser 
1               5           


<210>  9
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  9

Leu Gln Tyr Ala Ser Tyr Pro Trp Thr 
1               5                   


<210>  10
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  10

Glu Val Gln Leu Val Glu Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser 
            20                  25  


<210>  11
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  11

Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met Gly Trp 
1               5                   10                  15  


<210>  12
<211>  40
<212>  PRT
<213>  Homo sapiens

<400>  12

Thr Phe Ala Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr 
1               5                   10                  15      


Ser Ala Arg Thr Thr Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp 
            20                  25                  30          


Thr Ala Thr Tyr Phe Cys Ala Arg 
        35                  40  


<210>  13
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  13

Trp Gly Gln Gly Thr Ser Leu Thr Val Ser Ser 
1               5                   10      


<210>  14
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  14

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys 
            20              


<210>  15
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  15

Trp Leu Gln Gln Lys Pro Asp Gly Thr Ile Lys Arg Leu Ile Tyr 
1               5                   10                  15  


<210>  16
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  16

Gly Val Pro Lys Arg Phe Ser Gly Ser Arg Ser Gly Ser Asp Tyr Ser 
1               5                   10                  15      


Leu Thr Ile Ser Ser Leu Glu Ser Glu Asp Phe Ala Asp Tyr Tyr Cys 
            20                  25                  30          


<210>  17
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  17

Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro 
1               5                   10                  15      


Thr Val Ser 
            


<210>  18
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized VH domain FW1

<400>  18

Val Lys Leu Gln Glu Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Ala Ser 
            20                  


<210>  19
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VH domain FW3

<400>  19

Thr Tyr Ala Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr 
1               5                   10                  15      


Ser Ala Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp 
            20                  25                  30          


Thr Ala Thr Tyr Phe Cys Ala Arg 
        35                  40  


<210>  20
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VH domain FW4

<400>  20

Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
1               5                   10      


<210>  21
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VL domain FW1

<400>  21

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys 
            20              


<210>  22
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VL domain FW2

<400>  22

Trp Leu Gln Gln Glu Pro Asp Gly Thr Ile Lys Arg Leu Ile Tyr 
1               5                   10                  15  


<210>  23
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VL domain FW3

<400>  23

Gly Val Pro Lys Arg Phe Ser Gly Ser Arg Ser Gly Ser Asp Tyr Ser 
1               5                   10                  15      


Leu Thr Ile Ser Ser Leu Glu Ser Glu Asp Phe Val Asp Tyr Tyr Cys 
            20                  25                  30          


<210>  24
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VH domain

<400>  24

Val Lys Leu Gln Glu Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Ser 
            20                  25                  30          


Met His Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met Gly 
        35                  40                  45              


Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe Lys 
    50                  55                  60                  


Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr Leu 
65                  70                  75                  80  


Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys Ala 
                85                  90                  95      


Arg Thr Ala Val Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
            100                 105                 110         


<210>  25
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized VL domain

<400>  25

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys Arg Ala Ser Gln Glu Ile Ser Val Ser 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Glu Pro Asp Gly Thr Ile Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Thr Ser Thr Leu Asp Ser Gly Val Pro Lys Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Val Asp Tyr Tyr Cys Leu Gln Tyr Ala Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser 
        115     


<210>  26
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EpCAM F Primer

<400>  26
ctccacgtgc tggtgtgt                                                     18


<210>  27
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EpCAM R Primer

<400>  27
tgttttagtt caatgatgat ccagta                                            26


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  c-Myc F Primer

<400>  28
aaacacaaac ttgaacagct ac                                                22


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  c-Myc R Primer

<400>  29
atttgaggca gtttacatta tgg                                               23


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nanog F Primer

<400>  30
atgcctcaca cggagactgt                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nanog R Primer

<400>  31
agggctgtcc tgaataagca                                                   20


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sox2 F Primer

<400>  32
tatttgaatc agtctgccga g                                                 21


<210>  33
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sox2 R Primer

<400>  33
atgtacctgt tataaggatg atattagt                                          28


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4 F Primer

<400>  34
agcaaaaccc ggaggagt                                                     18


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oct4 R Primer

<400>  35
ccacatcggc ctgtgtatat c                                                 21


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E-cadherin F Primer

<400>  36
ggaactatga aaagtgggct tg                                                22


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E-cadherin R Primer

<400>  37
aaattgccag gctcaatgac                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vimentin F Primer

<400>  38
gtttccccta aaccgctagg                                                   20


<210>  39
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vimentin R Primer

<400>  39
agcgagagtg gcagagga                                                     18


<210>  40
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Snail F Primer

<400>  40
cttcggctcc aggagagtc                                                    19


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Snail R Primer

<400>  41
ttcccactgt cctcatctga c                                                 21


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Slug F Primer

<400>  42
tggttgcttc aaggacacat                                                   20


<210>  43
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Slug R Primer

<400>  43
gttgcagtga gggcaagaa                                                    19


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH F Primer

<400>  44
cttcaccacc atggaggagg c                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH R Primer

<400>  45
ggcatggact gtggtcatga g                                                 21


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18s rRNA F Primer

<400>  46
gcaattattc cccatgaacg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18s rRNA R Primer

<400>  47
gggacttaat caacgcaagc                                                   20


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primers c-Myc F

<400>  48
gcctgcgatg atttatactc ac                                                22


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primers c-Myc R

<400>  49
aaacagagta agagagccg                                                    19


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primers Nanog F

<400>  50
tcttcaggtt ctgttgctcg                                                   20


<210>  51
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primers Nanog R

<400>  51
gttaatcccg tctaccagtc tc                                                22


<210>  52
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP Primers Sox2 F

<400>  52
ggataacatt gtactgggaa gggaca                                            26


<210>  53
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primers Sox2 R

<400>  53
caaagtttct tttattcgta tgtgtgagca                                        30


<210>  54
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primer Oct F

<400>  54
cgcctcgagt ggggaacctg gaggatggca ag                                     32


<210>  55
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primer Oct4 R

<400>  55
tataagcttg gggaaggaag gcgccccaag                                        30


<210>  56
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP primers GAPDH F Primer

<400>  56
tccaagcgtg taagggt                                                      17


<210>  57
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP Primers GAPDH R Primer

<400>  57
gaagggactg agattggc                                                     18


<210>  58
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning Primers EpEX F

<400>  58
tataagctta ccatggcgcc cccgc                                             25


<210>  59
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning primers EpEX R

<400>  59
cgcctcgaga ataaccagca caa                                               23


<210>  60
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning primers EpCAM F

<400>  60
gataagctta tggcgccccc gcaggtc                                           27


<210>  61
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning primers EpCAM R

<400>  61
gatctcgagt gcattgagtt ccctatgcat ctcacc                                 36


<210>  62
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  62

Val Gly Ala Gln Asn Thr Val Ile Cys 
1               5                   


<210>  63
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  63

Lys Pro Glu Gly Ala Leu Gln Asn Asn Asp Gly Leu Tyr Asp Pro Asp 
1               5                   10                  15      


Cys Asp Glu 
            


